AlfalfaGEDB | MsG0480018522.01

M. sativa cultivar ZhongmuNo.1 / MsG0480018522.01

KEGG: -
GO: -
PFAM: PF03018

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018522.01.T01	AES86702.1	97.396	192	5	0	1	192	1	192	2.64E-133	381

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018522.01.T01	Q9C523	53.476	187	74	4	10	191	7	185	5.33E-64	198

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018522.01.T01	G7JKJ6	97.396	192	5	0	1	192	1	192	1.26e-133	381

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048036.01	MsG0480018522.01	0.821678	3.552841e-53	2.910005e-50
MsG0080048301.01	MsG0480018522.01	0.836648	8.272262e-57	1.048951e-53
MsG0080049154.01	MsG0480018522.01	0.800473	1.417701e-48	6.606865e-46
MsG0180000399.01	MsG0480018522.01	0.831514	1.599030e-55	1.739197e-52
MsG0180001084.01	MsG0480018522.01	0.826784	2.242638e-54	2.124055e-51
MsG0180001463.01	MsG0480018522.01	0.826292	2.938882e-54	2.744673e-51
MsG0180001533.01	MsG0480018522.01	0.867475	1.331939e-65	4.656427e-62
MsG0180001940.01	MsG0480018522.01	0.818984	1.473361e-52	1.119769e-49
MsG0180003235.01	MsG0480018522.01	0.822194	2.699022e-53	2.243278e-50
MsG0180003237.01	MsG0480018522.01	0.852200	5.396922e-61	1.119259e-57
MsG0180003320.01	MsG0480018522.01	0.821173	4.648596e-53	3.754208e-50
MsG0180003322.01	MsG0480018522.01	0.821985	3.017244e-53	2.492919e-50
MsG0180003604.01	MsG0480018522.01	0.811435	7.012746e-51	4.340662e-48
MsG0180004302.01	MsG0480018522.01	-0.820805	5.648437e-53	4.515287e-50
MsG0180004328.01	MsG0480018522.01	0.801518	8.671268e-49	4.149468e-46
MsG0180004424.01	MsG0480018522.01	0.847843	8.944820e-60	1.610549e-56
MsG0180004937.01	MsG0480018522.01	-0.826286	2.948739e-54	2.753444e-51
MsG0180004942.01	MsG0480018522.01	0.839817	1.262851e-57	1.764493e-54
MsG0180005358.01	MsG0480018522.01	0.802280	6.046982e-49	2.950248e-46
MsG0180005648.01	MsG0480018522.01	0.871902	4.794748e-67	1.966565e-63
MsG0280006442.01	MsG0480018522.01	0.858948	5.802267e-63	1.506712e-59
MsG0280010802.01	MsG0480018522.01	0.846959	1.564435e-59	2.738394e-56
MsG0280011238.01	MsG0480018522.01	0.801663	8.097782e-49	3.889100e-46
MsG0280011449.01	MsG0480018522.01	-0.804262	2.350688e-49	1.206511e-46
MsG0380011635.01	MsG0480018522.01	0.824773	6.729660e-54	6.019337e-51
MsG0380012254.01	MsG0480018522.01	0.829461	5.080796e-55	5.203597e-52
MsG0380012654.01	MsG0480018522.01	0.851417	8.994687e-61	1.819518e-57
MsG0480018521.01	MsG0480018522.01	0.929518	6.193181e-93	3.474987e-88
MsG0480018522.01	MsG0480018621.01	0.801908	7.210485e-49	3.484767e-46
MsG0480018522.01	MsG0480019217.01	0.834442	2.990606e-56	3.549474e-53
MsG0480018522.01	MsG0480019348.01	0.801417	9.093146e-49	4.340235e-46
MsG0480018522.01	MsG0480022300.01	-0.818998	1.462756e-52	1.112121e-49
MsG0480018522.01	MsG0480022308.01	0.800503	1.398123e-48	6.520620e-46
MsG0480018522.01	MsG0480022309.01	0.867763	1.076849e-65	3.803009e-62
MsG0480018522.01	MsG0480022310.01	0.830921	2.236772e-55	2.390456e-52
MsG0480018522.01	MsG0480022311.01	0.857830	1.249624e-62	3.124922e-59
MsG0480018522.01	MsG0480022638.01	0.830100	3.551592e-55	3.705363e-52
MsG0480018522.01	MsG0480022833.01	0.850731	1.404367e-60	2.777404e-57
MsG0480018522.01	MsG0480022880.01	0.826692	2.359696e-54	2.229018e-51
MsG0480018522.01	MsG0480023006.01	-0.802155	6.415254e-49	3.119953e-46
MsG0480018522.01	MsG0480023102.01	0.858665	7.048491e-63	1.812664e-59
MsG0480018522.01	MsG0480023107.01	0.801448	8.963307e-49	4.281589e-46
MsG0480018522.01	MsG0480023349.01	0.816385	5.684332e-52	4.021936e-49
MsG0480018522.01	MsG0480023693.01	0.850181	2.004742e-60	3.893962e-57
MsG0480018522.01	MsG0480023921.01	0.821702	3.507591e-53	2.874972e-50
MsG0480018522.01	MsG0580024086.01	0.858728	6.748858e-63	1.739430e-59
MsG0480018522.01	MsG0580024138.01	0.803759	2.990048e-49	1.515059e-46
MsG0480018522.01	MsG0580024176.01	0.808530	2.960326e-50	1.696729e-47
MsG0480018522.01	MsG0580024202.01	0.903155	4.643258e-79	6.786375e-75
MsG0480018522.01	MsG0580024207.01	0.888343	6.424475e-73	4.958544e-69
MsG0480018522.01	MsG0580024217.01	0.882094	1.389013e-70	8.349229e-67
MsG0480018522.01	MsG0580024218.01	0.824485	7.866789e-54	6.977465e-51
MsG0480018522.01	MsG0580024219.01	0.867910	9.656523e-66	3.427881e-62
MsG0480018522.01	MsG0580024220.01	0.826082	3.296974e-54	3.060715e-51
MsG0480018522.01	MsG0580024408.01	0.857900	1.190938e-62	2.985348e-59
MsG0480018522.01	MsG0580024986.01	0.926778	2.933724e-91	1.410785e-86
MsG0480018522.01	MsG0580024990.01	0.924019	1.231713e-89	5.087814e-85
MsG0480018522.01	MsG0580025965.01	0.916840	1.096314e-85	3.071140e-81
MsG0480018522.01	MsG0580026492.01	0.810938	8.988588e-51	5.491421e-48
MsG0480018522.01	MsG0580026521.01	0.800826	1.201307e-48	5.648813e-46
MsG0480018522.01	MsG0580026925.01	0.822863	1.886069e-53	1.597896e-50
MsG0480018522.01	MsG0580027751.01	0.811821	5.779314e-51	3.613796e-48
MsG0480018522.01	MsG0580029291.01	0.826758	2.276123e-54	2.154078e-51
MsG0480018522.01	MsG0580029664.01	0.841254	5.313530e-58	7.761976e-55
MsG0480018522.01	MsG0580029706.01	-0.814381	1.586068e-51	1.062638e-48
MsG0480018522.01	MsG0580029820.01	-0.821874	3.201300e-53	2.636814e-50
MsG0480018522.01	MsG0580030133.01	0.839361	1.659445e-57	2.286310e-54
MsG0480018522.01	MsG0580030135.01	0.881014	3.412552e-70	1.966540e-66
MsG0480018522.01	MsG0580030145.01	0.833684	4.628729e-56	5.369767e-53
MsG0480018522.01	MsG0680031009.01	0.880764	4.195216e-70	2.395491e-66
MsG0480018522.01	MsG0680031200.01	0.806391	8.414713e-50	4.561685e-47
MsG0480018522.01	MsG0680032026.01	0.841833	3.738267e-58	5.560853e-55
MsG0480018522.01	MsG0680032461.01	0.829106	6.196245e-55	6.279998e-52
MsG0480018522.01	MsG0680033754.01	0.805497	1.297232e-49	6.873360e-47
MsG0480018522.01	MsG0680034255.01	0.834738	2.519684e-56	3.016751e-53
MsG0480018522.01	MsG0680034316.01	0.824991	5.977870e-54	5.380232e-51
MsG0480018522.01	MsG0680034317.01	0.834476	2.931737e-56	3.483308e-53
MsG0480018522.01	MsG0680034649.01	0.802632	5.117617e-49	2.519252e-46
MsG0480018522.01	MsG0680034974.01	0.800835	1.195836e-48	5.624616e-46
MsG0480018522.01	MsG0780035932.01	0.809048	2.293311e-50	1.332526e-47
MsG0480018522.01	MsG0780036370.01	0.805141	1.540470e-49	8.088113e-47
MsG0480018522.01	MsG0780036376.01	0.801686	8.010997e-49	3.849619e-46
MsG0480018522.01	MsG0780036472.01	0.825507	4.514697e-54	4.123012e-51
MsG0480018522.01	MsG0780036661.01	0.827770	1.302440e-54	1.269511e-51
MsG0480018522.01	MsG0780036925.01	-0.833392	5.476212e-56	6.297924e-53
MsG0480018522.01	MsG0780036960.01	0.822643	2.122633e-53	1.787178e-50
MsG0480018522.01	MsG0780037917.01	0.817190	3.750621e-52	2.712786e-49
MsG0480018522.01	MsG0780038860.01	0.810529	1.101617e-50	6.656278e-48
MsG0480018522.01	MsG0780038922.01	0.805035	1.621317e-49	8.489168e-47
MsG0480018522.01	MsG0780038963.01	0.922374	1.067838e-88	4.024756e-84
MsG0480018522.01	MsG0780039576.01	0.837334	5.526649e-57	7.155679e-54
MsG0480018522.01	MsG0780039968.01	0.807057	6.088566e-50	3.357752e-47
MsG0480018522.01	MsG0780040615.01	0.904911	7.478703e-80	1.181582e-75
MsG0480018522.01	MsG0780040991.01	0.801010	1.101626e-48	5.204846e-46
MsG0480018522.01	MsG0780041447.01	0.858115	1.027811e-62	2.594940e-59
MsG0480018522.01	MsG0880042261.01	0.803477	3.420888e-49	1.720832e-46
MsG0480018522.01	MsG0880042895.01	-0.809832	1.556869e-50	9.237011e-48
MsG0480018522.01	MsG0880042921.01	-0.808123	3.615002e-50	2.049906e-47
MsG0480018522.01	MsG0880043481.01	0.805417	1.348428e-49	7.130049e-47
MsG0480018522.01	MsG0880045677.01	0.812216	4.740071e-51	2.995952e-48
MsG0480018522.01	MsG0880046429.01	0.810591	1.068134e-50	6.464889e-48
MsG0480018522.01	MsG0880046814.01	-0.822117	2.812634e-53	2.332602e-50
MsG0480018522.01	MsG0880047251.01	-0.801161	1.025807e-48	4.864958e-46
MsG0480018522.01	MsG0880047398.01	0.803728	3.035173e-49	1.536666e-46
MsG0480018522.01	MsG0880047542.01	0.873868	1.053331e-67	4.642085e-64
MsG0480018522.01	MsG0880047665.01	0.826063	3.332559e-54	3.092052e-51
MsG0380015080.01	MsG0480018522.01	0.814540	1.462908e-51	9.843002e-49
MsG0380015110.01	MsG0480018522.01	0.827633	1.404571e-54	1.363549e-51
MsG0380015541.01	MsG0480018522.01	0.812506	4.097586e-51	2.610028e-48
MsG0380016045.01	MsG0480018522.01	0.821341	4.250608e-53	3.448833e-50
MsG0380016086.01	MsG0480018522.01	0.887372	1.511861e-72	1.121384e-68
MsG0380016409.01	MsG0480018522.01	0.811877	5.620443e-51	3.519630e-48
MsG0380017151.01	MsG0480018522.01	0.852125	5.668163e-61	1.172696e-57
MsG0380017754.01	MsG0480018522.01	0.834908	2.282573e-56	2.747234e-53
MsG0380017860.01	MsG0480018522.01	0.818327	2.077139e-52	1.550051e-49
MsG0380018070.01	MsG0480018522.01	0.845268	4.514959e-59	7.483533e-56
MsG0480018439.01	MsG0480018522.01	0.809791	1.588890e-50	9.416991e-48
MsG0480018440.01	MsG0480018522.01	0.811186	7.939996e-51	4.882367e-48
MsG0280006964.01	MsG0480018522.01	-0.813577	2.385300e-51	1.563618e-48
MsG0280007716.01	MsG0480018522.01	0.800445	1.436595e-48	6.690330e-46
MsG0280007869.01	MsG0480018522.01	0.813404	2.604012e-51	1.699029e-48
MsG0280007906.01	MsG0480018522.01	0.910945	1.060537e-82	2.221347e-78
MsG0280007908.01	MsG0480018522.01	0.939304	1.592865e-99	1.623498e-94
MsG0280007909.01	MsG0480018522.01	0.834427	3.016159e-56	3.578391e-53
MsG0280007911.01	MsG0480018522.01	0.893147	8.235191e-75	7.753739e-71
MsG0280009667.01	MsG0480018522.01	0.844765	6.174046e-59	1.007084e-55
MsG0280009673.01	MsG0480018522.01	0.803894	2.803787e-49	1.425658e-46
MsG0280010005.01	MsG0480018522.01	0.817255	3.626512e-52	2.627686e-49
MsG0280010410.01	MsG0480018522.01	0.815030	1.139195e-51	7.767109e-49
MsG0280010417.01	MsG0480018522.01	0.801176	1.018833e-48	4.833570e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018522.01.T01	MTR_4g013770	97.396	192	5	0	1	192	1	192	3.19e-137	381
MsG0480018522.01.T01	MTR_4g013385	68.817	186	56	1	6	191	7	190	2.37e-89	260
MsG0480018522.01.T01	MTR_0433s0040	68.817	186	56	1	6	191	7	190	2.37e-89	260
MsG0480018522.01.T01	MTR_4g013355	66.667	186	51	2	6	191	5	179	6.40e-84	246
MsG0480018522.01.T01	MTR_4g013350	61.413	184	65	3	13	191	15	197	1.12e-70	213
MsG0480018522.01.T01	MTR_4g013345	60.109	183	68	3	13	191	15	196	2.90e-68	207
MsG0480018522.01.T01	MTR_4g013330	59.218	179	69	3	17	191	21	199	3.06e-68	207
MsG0480018522.01.T01	MTR_4g013335	61.765	170	60	2	24	191	30	196	7.03e-68	206
MsG0480018522.01.T01	MTR_7g070390	57.447	188	75	2	7	191	7	192	1.13e-66	202
MsG0480018522.01.T01	MTR_4g013320	56.497	177	74	2	17	191	24	199	5.42e-65	199
MsG0480018522.01.T01	MTR_4g013325	63.699	146	52	1	46	191	55	199	4.04e-64	196
MsG0480018522.01.T01	MTR_4g013310	57.143	168	66	4	24	188	30	194	4.82e-62	191
MsG0480018522.01.T01	MTR_1g054525	47.668	193	91	5	6	191	3	192	5.04e-54	171
MsG0480018522.01.T01	MTR_7g093790	42.077	183	99	5	11	191	14	191	3.26e-47	153
MsG0480018522.01.T01	MTR_7g093850	40.909	176	101	3	18	191	20	194	1.76e-44	146
MsG0480018522.01.T01	MTR_7g093820	39.773	176	103	3	18	191	20	194	1.09e-43	144
MsG0480018522.01.T01	MTR_4g073950	42.105	152	88	0	40	191	41	192	7.23e-43	142
MsG0480018522.01.T01	MTR_7g093830	38.068	176	106	3	18	191	19	193	1.51e-42	141
MsG0480018522.01.T01	MTR_1g056370	45.395	152	80	3	40	190	36	185	2.99e-42	140
MsG0480018522.01.T01	MTR_7g093870	42.857	168	91	3	29	191	26	193	2.97e-40	136
MsG0480018522.01.T01	MTR_3g105640	40.373	161	93	2	32	191	30	188	1.13e-34	121
MsG0480018522.01.T01	MTR_5g096120	39.267	191	102	5	7	191	7	189	3.88e-33	117
MsG0480018522.01.T01	MTR_3g105630	36.269	193	112	4	4	191	3	189	3.92e-33	117
MsG0480018522.01.T01	MTR_4g078885	36.735	147	90	1	45	191	42	185	7.87e-30	108
MsG0480018522.01.T01	MTR_3g034030	34.254	181	115	2	12	191	8	185	9.76e-30	108
MsG0480018522.01.T01	MTR_4g049550	34.483	145	88	2	45	186	33	173	1.50e-22	89.7
MsG0480018522.01.T01	MTR_4g122130	37.500	144	87	2	49	191	39	180	3.45e-22	89.0
MsG0480018522.01.T01	MTR_4g049570	33.793	145	89	2	45	186	33	173	1.88e-21	87.0
MsG0480018522.01.T01	MTR_4g122110	32.515	163	106	2	29	191	22	180	5.81e-21	85.5
MsG0480018522.01.T01	MTR_8g099135	32.298	161	83	5	30	174	18	168	1.27e-13	66.2
MsG0480018522.01.T01	MTR_1g046490	38.298	94	57	1	78	171	121	213	7.36e-13	65.1
MsG0480018522.01.T01	MTR_1g046800	39.362	94	56	1	78	171	135	227	2.49e-12	63.9
MsG0480018522.01.T01	MTR_1g046500	39.362	94	56	1	78	171	135	227	2.70e-12	63.9
MsG0480018522.01.T01	MTR_8g099115	33.333	135	78	4	49	174	41	172	1.79e-11	60.8
MsG0480018522.01.T01	MTR_8g073770	26.056	142	103	1	46	187	42	181	9.13e-11	58.9
MsG0480018522.01.T01	MTR_8g073850	26.056	142	103	1	46	187	42	181	1.00e-10	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018522.01.T01	AT1G58170	53.476	187	74	4	10	191	7	185	5.43e-65	198
MsG0480018522.01.T01	AT1G55210	51.323	189	85	5	6	191	3	187	4.04e-57	178
MsG0480018522.01.T01	AT1G55210	51.323	189	85	5	6	191	3	187	4.04e-57	178
MsG0480018522.01.T01	AT5G49040	50.847	177	84	2	17	191	16	191	9.08e-56	175
MsG0480018522.01.T01	AT3G13650	51.445	173	79	4	20	191	18	186	8.04e-53	167
MsG0480018522.01.T01	AT3G13662	49.425	174	78	5	7	178	7	172	5.28e-47	152
MsG0480018522.01.T01	AT1G65870	47.260	146	76	1	45	190	45	189	2.95e-45	148
MsG0480018522.01.T01	AT5G49030	50.602	166	79	2	17	180	16	180	3.49e-44	157
MsG0480018522.01.T01	AT3G13660	57.143	119	51	0	73	191	7	125	2.63e-42	139
MsG0480018522.01.T01	AT5G42500	41.818	165	93	2	26	190	24	185	1.80e-40	136
MsG0480018522.01.T01	AT1G22900	46.575	146	75	2	45	190	51	193	4.38e-39	132
MsG0480018522.01.T01	AT5G42510	41.875	160	90	2	31	190	26	182	5.06e-39	132
MsG0480018522.01.T01	AT2G21100	41.447	152	88	1	39	190	37	187	6.59e-38	129
MsG0480018522.01.T01	AT2G21100	41.447	152	88	1	39	190	37	187	6.59e-38	129
MsG0480018522.01.T01	AT3G58090	46.364	110	59	0	81	190	162	271	2.17e-32	117
MsG0480018522.01.T01	AT4G38700	34.694	196	117	5	1	191	1	190	1.99e-28	105
MsG0480018522.01.T01	AT2G21110	32.432	185	116	4	11	191	7	186	5.73e-28	103
MsG0480018522.01.T01	AT4G23690	36.029	136	77	5	36	166	32	162	2.24e-15	71.2
MsG0480018522.01.T01	AT4G11210	31.579	171	103	6	12	178	7	167	4.17e-14	67.8
MsG0480018522.01.T01	AT4G11190	30.935	139	85	4	44	178	36	167	1.42e-12	63.5

Find 48 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTAAAAGAGTTGTTAAATT+TGG	0.090030	4:+6108273	None:intergenic
AAGTTTGGTGTCAAGGTTAA+AGG	0.229203	4:+6108220	None:intergenic
AAGTAGCTGCTAGCCACTTT+AGG	0.300872	4:-6108360	MsG0480018522.01.T01:CDS
CTAAAAGAGTTGTTAAATTT+GGG	0.308915	4:+6108274	None:intergenic
TTGGGAAGTGGTTGAATGAT+TGG	0.327500	4:+6108292	None:intergenic
TTGTTTGAAGGAAAGTATAA+TGG	0.337558	4:-6108109	MsG0480018522.01.T01:CDS
GGGTGTAGAAAGAGAGTAGA+AGG	0.394648	4:+6108452	None:intergenic
GGGAGTACCATTACTATCTC+TGG	0.407901	4:-6108088	MsG0480018522.01.T01:CDS
ATGAACTTTGCCTTGTTTGA+AGG	0.417944	4:-6108121	MsG0480018522.01.T01:CDS
TGTTTGAAGGAAAGTATAAT+GGG	0.433452	4:-6108108	MsG0480018522.01.T01:CDS
CAGCATATCCCTTAGCAAAT+CGG	0.436333	4:+6108005	None:intergenic
ATTTATTGAGCTTACAAAAC+CGG	0.438981	4:+6108402	None:intergenic
AGTTTGGTGTCAAGGTTAAA+GGG	0.445162	4:+6108221	None:intergenic
TACCAGTGATCGGTGGTAGC+GGG	0.446390	4:-6108033	MsG0480018522.01.T01:CDS
CTAGCCACTTTAGGTTCTAC+TGG	0.448964	4:-6108351	MsG0480018522.01.T01:CDS
AACTTGACCTCAAAATTGTT+AGG	0.452723	4:-6108202	MsG0480018522.01.T01:CDS
TACTTTCTTTGTAAGCAACT+TGG	0.452780	4:+6108378	None:intergenic
AAGGAACGTCGCAAACGATA+AGG	0.481824	4:-6108065	MsG0480018522.01.T01:CDS
ACTTTCTTTGTAAGCAACTT+GGG	0.483229	4:+6108379	None:intergenic
TGACCTCAAAATTGTTAGGT+AGG	0.497868	4:-6108198	MsG0480018522.01.T01:CDS
TACACCCTCACAATTGTCAC+TGG	0.498118	4:-6108436	MsG0480018522.01.T01:CDS
AAATTGTTAGGTAGGGCTCA+AGG	0.500966	4:-6108190	MsG0480018522.01.T01:CDS
TGAGGTCAAGTTTGGTGTCA+AGG	0.506209	4:+6108213	None:intergenic
GAGTTGTTAAATTTGGGAAG+TGG	0.508093	4:+6108280	None:intergenic
GTTCTACTGGCAAGACATAG+TGG	0.508556	4:-6108338	MsG0480018522.01.T01:CDS
ACCCCGCTACCACCGATCAC+TGG	0.532338	4:+6108031	None:intergenic
TTACCAGTGATCGGTGGTAG+CGG	0.542224	4:-6108034	MsG0480018522.01.T01:CDS
AATTGTTAGGTAGGGCTCAA+GGG	0.543701	4:-6108189	MsG0480018522.01.T01:CDS
TGGTTGAATGATTGGAATGG+AGG	0.546721	4:+6108300	None:intergenic
GTATTCAACCACGGTATCCC+CGG	0.549581	4:+6107952	None:intergenic
CTTGCCAGTAGAACCTAAAG+TGG	0.555578	4:+6108347	None:intergenic
GACCTCAAAATTGTTAGGTA+GGG	0.562175	4:-6108197	MsG0480018522.01.T01:CDS
ACCAGTGATCGGTGGTAGCG+GGG	0.577667	4:-6108032	MsG0480018522.01.T01:CDS
GTCACTGGACAAGATGAGAC+CGG	0.584277	4:-6108421	MsG0480018522.01.T01:CDS
AAGTGGTTGAATGATTGGAA+TGG	0.586361	4:+6108297	None:intergenic
ATTATACTTTCCTTCAAACA+AGG	0.587601	4:+6108111	None:intergenic
AACCATAAGAAAATCAAGCT+CGG	0.592742	4:+6108144	None:intergenic
GTTAGAGAGTTACCAGTGAT+CGG	0.602234	4:-6108043	MsG0480018522.01.T01:CDS
TTCTACTGGCAAGACATAGT+GGG	0.624056	4:-6108337	MsG0480018522.01.T01:CDS
TTTCATAACCGGGGATACCG+TGG	0.641517	4:-6107960	MsG0480018522.01.T01:CDS
GTACCATTACTATCTCTGGA+AGG	0.647934	4:-6108084	MsG0480018522.01.T01:CDS
ATAAACATTGTATTCAACCA+CGG	0.650255	4:+6107943	None:intergenic
GTTCCTTCCAGAGATAGTAA+TGG	0.662479	4:+6108081	None:intergenic
AAGCTCGGTTTGCGATGTAG+CGG	0.665030	4:+6108159	None:intergenic
TACTGGCAAGACATAGTGGG+AGG	0.669570	4:-6108334	MsG0480018522.01.T01:CDS
CTTGTCCAGTGACAATTGTG+AGG	0.674513	4:+6108431	None:intergenic
TTGTCCAGTGACAATTGTGA+GGG	0.677874	4:+6108432	None:intergenic
AGAGAGTTACCAGTGATCGG+TGG	0.703961	4:-6108040	MsG0480018522.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACTAAAAGAGTTGTTAAATT+TGG	+	Chr4:6108136-6108155	None:intergenic	20.0%
!!	CTAAAAGAGTTGTTAAATTT+GGG	+	Chr4:6108135-6108154	None:intergenic	20.0%
!!!	AAGGAAGAAAAAAAGTTTTT+GGG	+	Chr4:6107938-6107957	None:intergenic	20.0%
!	ATAAACATTGTATTCAACCA+CGG	+	Chr4:6108466-6108485	None:intergenic	25.0%
!	ATTATACTTTCCTTCAAACA+AGG	+	Chr4:6108298-6108317	None:intergenic	25.0%
!	ATTTATTGAGCTTACAAAAC+CGG	+	Chr4:6108007-6108026	None:intergenic	25.0%
!!	GAAGGAAGAAAAAAAGTTTT+TGG	+	Chr4:6107939-6107958	None:intergenic	25.0%
!!	TGTTTGAAGGAAAGTATAAT+GGG	-	Chr4:6108298-6108317	MsG0480018522.01.T01:CDS	25.0%
!!	TTGTTTGAAGGAAAGTATAA+TGG	-	Chr4:6108297-6108316	MsG0480018522.01.T01:CDS	25.0%
!!!	ATCTCTTTTGATTTCATAAC+CGG	-	Chr4:6108435-6108454	MsG0480018522.01.T01:CDS	25.0%
	AACCATAAGAAAATCAAGCT+CGG	+	Chr4:6108265-6108284	None:intergenic	30.0%
	AACTTGACCTCAAAATTGTT+AGG	-	Chr4:6108204-6108223	MsG0480018522.01.T01:CDS	30.0%
	ACTTTCTTTGTAAGCAACTT+GGG	+	Chr4:6108030-6108049	None:intergenic	30.0%
	TACTTTCTTTGTAAGCAACT+TGG	+	Chr4:6108031-6108050	None:intergenic	30.0%
!	AACAACTCTTTTAGTGCTTT+TGG	-	Chr4:6108141-6108160	MsG0480018522.01.T01:CDS	30.0%
!!!	AACAATTTTGAGGTCAAGTT+TGG	+	Chr4:6108204-6108223	None:intergenic	30.0%
!!!	TCTCTTTTGATTTCATAACC+GGG	-	Chr4:6108436-6108455	MsG0480018522.01.T01:CDS	30.0%
	AAGTGGTTGAATGATTGGAA+TGG	+	Chr4:6108112-6108131	None:intergenic	35.0%
	GACCTCAAAATTGTTAGGTA+GGG	-	Chr4:6108209-6108228	MsG0480018522.01.T01:CDS	35.0%
	GAGTTGTTAAATTTGGGAAG+TGG	+	Chr4:6108129-6108148	None:intergenic	35.0%
	TGACCTCAAAATTGTTAGGT+AGG	-	Chr4:6108208-6108227	MsG0480018522.01.T01:CDS	35.0%
!	AACCGAGCTTGATTTTCTTA+TGG	-	Chr4:6108260-6108279	MsG0480018522.01.T01:CDS	35.0%
!!	AAGTTTGGTGTCAAGGTTAA+AGG	+	Chr4:6108189-6108208	None:intergenic	35.0%
!!	AGTTTGGTGTCAAGGTTAAA+GGG	+	Chr4:6108188-6108207	None:intergenic	35.0%
!!	ATGAACTTTGCCTTGTTTGA+AGG	-	Chr4:6108285-6108304	MsG0480018522.01.T01:CDS	35.0%
!!	CTCTTTTAGTGCTTTTGGTT+TGG	-	Chr4:6108146-6108165	MsG0480018522.01.T01:CDS	35.0%
!!!	CTCTTTTGATTTCATAACCG+GGG	-	Chr4:6108437-6108456	MsG0480018522.01.T01:CDS	35.0%
	AAATTGTTAGGTAGGGCTCA+AGG	-	Chr4:6108216-6108235	MsG0480018522.01.T01:CDS	40.0%
	AATTGTTAGGTAGGGCTCAA+GGG	-	Chr4:6108217-6108236	MsG0480018522.01.T01:CDS	40.0%
	CAGCATATCCCTTAGCAAAT+CGG	+	Chr4:6108404-6108423	None:intergenic	40.0%
	GTACCATTACTATCTCTGGA+AGG	-	Chr4:6108322-6108341	MsG0480018522.01.T01:CDS	40.0%
	GTTAGAGAGTTACCAGTGAT+CGG	-	Chr4:6108363-6108382	MsG0480018522.01.T01:CDS	40.0%
	GTTCCTTCCAGAGATAGTAA+TGG	+	Chr4:6108328-6108347	None:intergenic	40.0%
	TGGTTGAATGATTGGAATGG+AGG	+	Chr4:6108109-6108128	None:intergenic	40.0%
	TTCTACTGGCAAGACATAGT+GGG	-	Chr4:6108069-6108088	MsG0480018522.01.T01:CDS	40.0%
	TTGGGAAGTGGTTGAATGAT+TGG	+	Chr4:6108117-6108136	None:intergenic	40.0%
	TTGTCCAGTGACAATTGTGA+GGG	+	Chr4:6107977-6107996	None:intergenic	40.0%
!	AGCCCTACCTAACAATTTTG+AGG	+	Chr4:6108214-6108233	None:intergenic	40.0%
!!!	TTAGTGCTTTTGGTTTGGTG+AGG	-	Chr4:6108151-6108170	MsG0480018522.01.T01:CDS	40.0%
	AAGGAACGTCGCAAACGATA+AGG	-	Chr4:6108341-6108360	MsG0480018522.01.T01:CDS	45.0%
	CTTGCCAGTAGAACCTAAAG+TGG	+	Chr4:6108062-6108081	None:intergenic	45.0%
	CTTGTCCAGTGACAATTGTG+AGG	+	Chr4:6107978-6107997	None:intergenic	45.0%
	GGGTGTAGAAAGAGAGTAGA+AGG	+	Chr4:6107957-6107976	None:intergenic	45.0%
	GTTCTACTGGCAAGACATAG+TGG	-	Chr4:6108068-6108087	MsG0480018522.01.T01:CDS	45.0%
	TACACCCTCACAATTGTCAC+TGG	-	Chr4:6107970-6107989	MsG0480018522.01.T01:CDS	45.0%
!	AAGTAGCTGCTAGCCACTTT+AGG	-	Chr4:6108046-6108065	MsG0480018522.01.T01:CDS	45.0%
!	CTAGCCACTTTAGGTTCTAC+TGG	-	Chr4:6108055-6108074	MsG0480018522.01.T01:CDS	45.0%
!!	GGGAGTACCATTACTATCTC+TGG	-	Chr4:6108318-6108337	MsG0480018522.01.T01:CDS	45.0%
!!	TGAGGTCAAGTTTGGTGTCA+AGG	+	Chr4:6108196-6108215	None:intergenic	45.0%
!!!	GGGGTTTTCCGATTTGCTAA+GGG	-	Chr4:6108393-6108412	MsG0480018522.01.T01:CDS	45.0%
	AGAGAGTTACCAGTGATCGG+TGG	-	Chr4:6108366-6108385	MsG0480018522.01.T01:CDS	50.0%
	GTATTCAACCACGGTATCCC+CGG	+	Chr4:6108457-6108476	None:intergenic	50.0%
	GTCACTGGACAAGATGAGAC+CGG	-	Chr4:6107985-6108004	MsG0480018522.01.T01:CDS	50.0%
	TTACCAGTGATCGGTGGTAG+CGG	-	Chr4:6108372-6108391	MsG0480018522.01.T01:CDS	50.0%
	TTTCATAACCGGGGATACCG+TGG	-	Chr4:6108446-6108465	MsG0480018522.01.T01:CDS	50.0%
!	AAGCTCGGTTTGCGATGTAG+CGG	+	Chr4:6108250-6108269	None:intergenic	50.0%
!	TACTGGCAAGACATAGTGGG+AGG	-	Chr4:6108072-6108091	MsG0480018522.01.T01:CDS	50.0%
!!!	CGGGGTTTTCCGATTTGCTA+AGG	-	Chr4:6108392-6108411	MsG0480018522.01.T01:CDS	50.0%
	TACCAGTGATCGGTGGTAGC+GGG	-	Chr4:6108373-6108392	MsG0480018522.01.T01:CDS	55.0%
!	ACCAGTGATCGGTGGTAGCG+GGG	-	Chr4:6108374-6108393	MsG0480018522.01.T01:CDS	60.0%
	ACCCCGCTACCACCGATCAC+TGG	+	Chr4:6108378-6108397	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	6107925	6108503	6107925	ID=MsG0480018522.01;Name=MsG0480018522.01
Chr4	mRNA	6107925	6108503	6107925	ID=MsG0480018522.01.T01;Parent=MsG0480018522.01;Name=MsG0480018522.01.T01;_AED=0.41;_eAED=0.41;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|192
Chr4	exon	6107925	6108503	6107925	ID=MsG0480018522.01.T01:exon:4188;Parent=MsG0480018522.01.T01
Chr4	CDS	6107925	6108503	6107925	ID=MsG0480018522.01.T01:cds;Parent=MsG0480018522.01.T01

Gene Sequence

>MsG0480018522.01.T01

ATGAATACTTCCCAAAAACTTTTTTTCTTCCTTCTACTCTCTTTCTACACCCTCACAATTGTCACTGGACAAGATGAGACCGGTTTTGTAAGCTCAATAAATCCCAAGTTGCTTACAAAGAAAGTAGCTGCTAGCCACTTTAGGTTCTACTGGCAAGACATAGTGGGAGGAGCAAACGCAACCTCCATTCCAATCATTCAACCACTTCCCAAATTTAACAACTCTTTTAGTGCTTTTGGTTTGGTGAGGATAATCGACAACCCTTTAACCTTGACACCAAACTTGACCTCAAAATTGTTAGGTAGGGCTCAAGGGTTTTATGCCGCTACATCGCAAACCGAGCTTGATTTTCTTATGGTTATGAACTTTGCCTTGTTTGAAGGAAAGTATAATGGGAGTACCATTACTATCTCTGGAAGGAACGTCGCAAACGATAAGGTTAGAGAGTTACCAGTGATCGGTGGTAGCGGGGTTTTCCGATTTGCTAAGGGATATGCTGAAGCTAGAACAATCTCTTTTGATTTCATAACCGGGGATACCGTGGTTGAATACAATGTTTATGTTTCTCATCATAAATGA

Protein sequence

>MsG0480018522.01.T01

MNTSQKLFFFLLLSFYTLTIVTGQDETGFVSSINPKLLTKKVAASHFRFYWQDIVGGANATSIPIIQPLPKFNNSFSAFGLVRIIDNPLTLTPNLTSKLLGRAQGFYAATSQTELDFLMVMNFALFEGKYNGSTITISGRNVANDKVRELPVIGGSGVFRFAKGYAEARTISFDFITGDTVVEYNVYVSHHK*