AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018177.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018177.01.T01 MTR_3g464580 83.333 48 8 0 3 50 31 78 1.79e-21 90.1
MsG0480018177.01.T01 MTR_3g464580 83.333 48 8 0 3 50 31 78 1.82e-21 90.5
MsG0480018177.01.T01 MTR_3g464580 83.333 48 8 0 3 50 31 78 1.95e-21 90.1
MsG0480018177.01.T01 MTR_5g071360 79.167 48 10 0 3 50 31 78 3.21e-20 87.0
MsG0480018177.01.T01 MTR_5g071360 79.167 48 10 0 3 50 31 78 3.49e-20 86.7
MsG0480018177.01.T01 MTR_3g087220 68.750 48 15 0 3 50 31 78 3.18e-16 75.5
MsG0480018177.01.T01 MTR_5g087870 62.500 64 21 1 36 99 404 464 6.03e-15 72.0
MsG0480018177.01.T01 MTR_5g087870 62.500 64 21 1 36 99 404 464 6.62e-15 71.6
MsG0480018177.01.T01 MTR_3g078647 59.375 64 23 1 36 99 379 439 1.30e-14 70.9
MsG0480018177.01.T01 MTR_5g087890 46.429 84 34 2 16 99 342 414 6.75e-12 63.2
MsG0480018177.01.T01 MTR_3g083300 72.500 40 11 0 60 99 382 421 5.89e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018177.01.T01 AT3G47340 70.833 48 14 0 3 50 31 78 6.07e-19 83.2
MsG0480018177.01.T01 AT3G47340 70.833 48 14 0 3 50 31 78 7.75e-19 83.2
MsG0480018177.01.T01 AT3G47340 70.833 48 14 0 3 50 31 78 8.03e-19 82.8
MsG0480018177.01.T01 AT5G10240 66.667 48 16 0 3 50 31 78 3.64e-15 72.4
MsG0480018177.01.T01 AT5G10240 66.667 48 16 0 3 50 31 78 3.71e-15 72.4
MsG0480018177.01.T01 AT5G65010 62.500 48 18 0 3 50 31 78 1.21e-14 70.9
MsG0480018177.01.T01 AT5G65010 62.500 48 18 0 3 50 31 78 1.21e-14 70.9
MsG0480018177.01.T01 AT2G27040 53.968 63 26 1 36 98 381 440 1.34e-11 62.4
MsG0480018177.01.T01 AT2G27040 53.968 63 26 1 36 98 381 440 1.34e-11 62.4

Find 60 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCATTGTTGATTCTGCTTC+TGG 0.237345 4:-1234018 MsG0480018177.01.T01:CDS
AGACAAATCAATTATTGTTC+TGG 0.260232 4:-1233974 MsG0480018177.01.T01:CDS
CCAACATGGTGATGACTATT+TGG 0.278820 4:-1234055 MsG0480018177.01.T01:CDS
TGGTGGAGCCCACTCCAGTC+TGG 0.295762 4:+1234075 None:intergenic
TTGGGGAGAGAGAGAAAATT+GGG 0.315927 4:-1230782 MsG0480018177.01.T01:CDS
TTGGAGTGTGGGCAGGGCTT+TGG 0.335047 4:+1233088 None:intergenic
AAAGAGGAATTGACGGATCT+TGG 0.339190 4:-1230801 MsG0480018177.01.T01:CDS
AGGACCTTTGGAGTGTGGGC+AGG 0.341217 4:+1233081 None:intergenic
CTTGGGGAGAGAGAGAAAAT+TGG 0.341385 4:-1230783 MsG0480018177.01.T01:CDS
TCTTCACAAGGGAGGACCTT+TGG 0.355434 4:+1233069 None:intergenic
GACAAATCAATTATTGTTCT+GGG 0.365959 4:-1233973 MsG0480018177.01.T01:CDS
AGAAAATTGGGGAGAGAGAA+TGG 0.370972 4:-1230770 MsG0480018177.01.T01:CDS
AAGAGGAATTGACGGATCTT+GGG 0.371737 4:-1230800 MsG0480018177.01.T01:CDS
GGACCTTTGGAGTGTGGGCA+GGG 0.402108 4:+1233082 None:intergenic
TCATAGATAAAGATGGAATC+TGG 0.410910 4:+1230903 None:intergenic
GAGAATGGAAAAGTAGTAAT+GGG 0.419805 4:-1230755 MsG0480018177.01.T01:CDS
GGTGGAGCCCACTCCAGTCT+GGG 0.459596 4:+1234076 None:intergenic
AGATTCCATCTTTATCTATG+AGG 0.461675 4:-1230901 MsG0480018177.01.T01:CDS
GAAGTGTCTCGGTGGCCATT+CGG 0.464702 4:+1230925 None:intergenic
AGAGAATGGAAAAGTAGTAA+TGG 0.478460 4:-1230756 MsG0480018177.01.T01:CDS
GGGCTTTGGTGTAACATTGA+AGG 0.483853 4:+1233102 None:intergenic
GAATGGACCGTGGCCCAGAC+TGG 0.486813 4:-1234089 MsG0480018177.01.T01:CDS
AGAAGCCTCAAGAGCAGATG+AGG 0.488444 4:-1233037 MsG0480018177.01.T01:CDS
GGAGAAGAAAGAGGAATTGA+CGG 0.503111 4:-1230808 MsG0480018177.01.T01:CDS
CCTCCCTTGTGAAGAAGTCT+AGG 0.515464 4:-1233061 MsG0480018177.01.T01:CDS
GCCCACTCCAGTCTGGGCCA+CGG 0.525119 4:+1234082 None:intergenic
GGCTTTGGTGTAACATTGAA+GGG 0.530378 4:+1233103 None:intergenic
ACCGTGGCCCAGACTGGAGT+GGG 0.540984 4:-1234083 MsG0480018177.01.T01:CDS
AAGACCCTCATCTGCTCTTG+AGG 0.541011 4:+1233032 None:intergenic
AATAGTCATCACCATGTTGG+TGG 0.545301 4:+1234058 None:intergenic
TCGTTCCTCATAGATAAAGA+TGG 0.546006 4:+1230896 None:intergenic
ATGACTATTTGGCTCATCAA+AGG 0.546458 4:-1234044 MsG0480018177.01.T01:CDS
AGGGAGGACCTTTGGAGTGT+GGG 0.550613 4:+1233077 None:intergenic
CCACATCAAATATGCAGCAC+AGG 0.553723 4:-1230862 MsG0480018177.01.T01:CDS
AGAGCAGATGAGGGTCTTGA+CGG 0.560421 4:-1233027 MsG0480018177.01.T01:CDS
GACCGTGGCCCAGACTGGAG+TGG 0.571259 4:-1234084 MsG0480018177.01.T01:CDS
AAGGGAGGACCTTTGGAGTG+TGG 0.573278 4:+1233076 None:intergenic
TCACCAGAAACGCCGCCGAA+TGG 0.575604 4:-1230940 MsG0480018177.01.T01:intron
AGAGGAATTGACGGATCTTG+GGG 0.581809 4:-1230799 MsG0480018177.01.T01:CDS
TGGGGAGAGAGAGAAAATTG+GGG 0.586510 4:-1230781 MsG0480018177.01.T01:CDS
AAGCCCTGCCCACACTCCAA+AGG 0.597838 4:-1233085 MsG0480018177.01.T01:CDS
CGACGGTGTGGAGAAGAAAG+AGG 0.598705 4:-1230817 MsG0480018177.01.T01:CDS
GATTTGTCTTCACTGAAGAG+AGG 0.600995 4:+1233988 None:intergenic
GTGTCTCGGTGGCCATTCGG+CGG 0.602622 4:+1230928 None:intergenic
CCAAATAGTCATCACCATGT+TGG 0.608389 4:+1234055 None:intergenic
GATGGAATCTGGAAGTGTCT+CGG 0.609927 4:+1230914 None:intergenic
GAAGCCTCAAGAGCAGATGA+GGG 0.640454 4:-1233036 MsG0480018177.01.T01:CDS
TGGAGTGGGCTCCACCAACA+TGG 0.641715 4:-1234069 MsG0480018177.01.T01:CDS
TGATCTCAGTTTCGCAACTG+AGG 0.642571 4:-1233929 MsG0480018177.01.T01:intron
TGACTCTGACAACGACGGTG+TGG 0.648622 4:-1230829 MsG0480018177.01.T01:CDS
TGCATATTTGATGTGGTGAG+CGG 0.650305 4:+1230869 None:intergenic
ACCAGAAGCAGAATCAACAA+TGG 0.683453 4:+1234017 None:intergenic
CCTAGACTTCTTCACAAGGG+AGG 0.685125 4:+1233061 None:intergenic
TCTGCCTAGACTTCTTCACA+AGG 0.686387 4:+1233057 None:intergenic
ATATTTGATGTGGTGAGCGG+AGG 0.692738 4:+1230872 None:intergenic
TGCTGCCGTTGAATGGACCG+TGG 0.694251 4:-1234099 None:intergenic
CTGCCTAGACTTCTTCACAA+GGG 0.694383 4:+1233058 None:intergenic
GAAAATGACTCTGACAACGA+CGG 0.699509 4:-1230834 MsG0480018177.01.T01:CDS
CCTGTGCTGCATATTTGATG+TGG 0.706971 4:+1230862 None:intergenic
GGAATCTGGAAGTGTCTCGG+TGG 0.718363 4:+1230917 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAAAAGAAAAAAAAAC+AGG + Chr4:1231978-1231997 None:intergenic 15.0%
!!! AATAGTATTGATAACAAATT+AGG + Chr4:1232052-1232071 None:intergenic 15.0%
!!! ATGTTTTTTTAAAGCTTAAA+AGG - Chr4:1232130-1232149 MsG0480018177.01.T01:intron 15.0%
!!! TTTTTCTTTTTTTCTTTTGA+GGG - Chr4:1231983-1232002 MsG0480018177.01.T01:intron 15.0%
!!! TTTTTTCTTTTTTTCTTTTG+AGG - Chr4:1231982-1232001 MsG0480018177.01.T01:intron 15.0%
!! AATGTTCCTTATTAATTGAT+TGG - Chr4:1232096-1232115 MsG0480018177.01.T01:intron 20.0%
!! ACTAACTAATACACATTATT+AGG + Chr4:1231412-1231431 None:intergenic 20.0%
!! ATATTCAACTATAATATACG+AGG + Chr4:1233009-1233028 None:intergenic 20.0%
!! GTTCCTATTTAATGTATATA+AGG - Chr4:1231556-1231575 MsG0480018177.01.T01:intron 20.0%
!! TCAGCTATTATGTATTATTT+AGG - Chr4:1231466-1231485 MsG0480018177.01.T01:intron 20.0%
!! TTGATTATGAATAAGGATTT+AGG - Chr4:1231040-1231059 MsG0480018177.01.T01:intron 20.0%
!!! AAGTTTGTTAATTCATTCAT+TGG + Chr4:1231608-1231627 None:intergenic 20.0%
!!! AGTAGATTTTATGTATTGAT+TGG + Chr4:1232902-1232921 None:intergenic 20.0%
!!! CTATTTTACTTTACAATCTA+TGG + Chr4:1231209-1231228 None:intergenic 20.0%
! AAAATTGCTATGATAAGACA+TGG - Chr4:1232259-1232278 MsG0480018177.01.T01:intron 25.0%
! AACTTGAGACAATCATTAAT+TGG - Chr4:1232683-1232702 MsG0480018177.01.T01:intron 25.0%
! AAGGAAAAATATAACACTTC+AGG - Chr4:1233123-1233142 MsG0480018177.01.T01:intron 25.0%
! AAGTAATTCAACATTAAGAG+AGG + Chr4:1232021-1232040 None:intergenic 25.0%
! ACAAGTAAAACAAAAAGGAA+AGG + Chr4:1233459-1233478 None:intergenic 25.0%
! ACTTGAGACAATCATTAATT+GGG - Chr4:1232684-1232703 MsG0480018177.01.T01:intron 25.0%
! AGACAAATCAATTATTGTTC+TGG - Chr4:1230865-1230884 MsG0480018177.01.T01:CDS 25.0%
! ATTAAATTCATCAACTCAAG+TGG + Chr4:1233240-1233259 None:intergenic 25.0%
! CAATCTAATATCAATCTAAG+AGG - Chr4:1230945-1230964 MsG0480018177.01.T01:intron 25.0%
! CTCTTAGATTGATATTAGAT+TGG + Chr4:1230947-1230966 None:intergenic 25.0%
! CTTTACTGTTAAGATTAACA+AGG - Chr4:1231516-1231535 MsG0480018177.01.T01:intron 25.0%
! GACAAATCAATTATTGTTCT+GGG - Chr4:1230866-1230885 MsG0480018177.01.T01:CDS 25.0%
! GCACCTTATATACATTAAAT+AGG + Chr4:1231562-1231581 None:intergenic 25.0%
! GGATTAACTAAATATTTGCT+AGG - Chr4:1232325-1232344 MsG0480018177.01.T01:intron 25.0%
! TGTGTGATTGATTATGAATA+AGG - Chr4:1231033-1231052 MsG0480018177.01.T01:intron 25.0%
! TTAAATTCATCAACTCAAGT+GGG + Chr4:1233239-1233258 None:intergenic 25.0%
! TTGTCACAAGTAAAACAAAA+AGG + Chr4:1233464-1233483 None:intergenic 25.0%
! TTTAATGAATCCTAGCAATT+AGG + Chr4:1232157-1232176 None:intergenic 25.0%
!! AGGTAGTAGAGATTTTTAAA+TGG - Chr4:1231486-1231505 MsG0480018177.01.T01:intron 25.0%
!! AGTGTTATATTTTTCCTTAG+AGG + Chr4:1233121-1233140 None:intergenic 25.0%
!! ATATATTAGCTTAGTGTTCA+TGG - Chr4:1232807-1232826 MsG0480018177.01.T01:intron 25.0%
!! GTGTTATATTTTTCCTTAGA+GGG + Chr4:1233120-1233139 None:intergenic 25.0%
!! TGTTATATTTTTCCTTAGAG+GGG + Chr4:1233119-1233138 None:intergenic 25.0%
!! TTTAATGTATATAAGGTGCT+TGG - Chr4:1231563-1231582 MsG0480018177.01.T01:intron 25.0%
!!! AATATCATTTGCTGATTTAC+AGG - Chr4:1231894-1231913 MsG0480018177.01.T01:intron 25.0%
!!! ACTTGAGTTGATGAATTTAA+TGG - Chr4:1233239-1233258 MsG0480018177.01.T01:intron 25.0%
!!! TGAGTTATTTTGACTTCTTA+AGG - Chr4:1233331-1233350 MsG0480018177.01.T01:intron 25.0%
!!! TTTATTCACTTTTCTTGAAG+TGG + Chr4:1233512-1233531 None:intergenic 25.0%
AAGGAAAAGTCTAACTTGAT+TGG - Chr4:1232541-1232560 MsG0480018177.01.T01:intron 30.0%
AATCATCATCCTTCTTTAAG+AGG + Chr4:1233569-1233588 None:intergenic 30.0%
AGAGAATGGAAAAGTAGTAA+TGG - Chr4:1234083-1234102 MsG0480018177.01.T01:CDS 30.0%
AGATGTAGTGTGCTATAATA+GGG + Chr4:1232774-1232793 None:intergenic 30.0%
AGATTCCATCTTTATCTATG+AGG - Chr4:1233938-1233957 MsG0480018177.01.T01:CDS 30.0%
ATAATATACGAGGATGTGTT+CGG + Chr4:1232999-1233018 None:intergenic 30.0%
GACAATGAATTGAAGAAGAT+CGG + Chr4:1233804-1233823 None:intergenic 30.0%
GAGAATGGAAAAGTAGTAAT+GGG - Chr4:1234084-1234103 MsG0480018177.01.T01:CDS 30.0%
GAGTTGTTGAGTGATAATAA+TGG - Chr4:1233353-1233372 MsG0480018177.01.T01:intron 30.0%
GATAAGACTGAGAAGAATAT+TGG + Chr4:1233700-1233719 None:intergenic 30.0%
GGGAATTATTCTTCATTGTT+TGG + Chr4:1233731-1233750 None:intergenic 30.0%
GGGTACTTAATTCACTTTAA+AGG + Chr4:1233098-1233117 None:intergenic 30.0%
TAGATGTAGTGTGCTATAAT+AGG + Chr4:1232775-1232794 None:intergenic 30.0%
TCACTGCCAATCAATTAATA+AGG + Chr4:1232105-1232124 None:intergenic 30.0%
TCATAGATAAAGATGGAATC+TGG + Chr4:1233939-1233958 None:intergenic 30.0%
! AATCAAGTTAGACTTTTCCT+TGG + Chr4:1232542-1232561 None:intergenic 30.0%
! GTTATATTTTTCCTTAGAGG+GGG + Chr4:1233118-1233137 None:intergenic 30.0%
!! ATTAAAGATTTGTTGAGTGG+TGG - Chr4:1233213-1233232 MsG0480018177.01.T01:intron 30.0%
!! ATTGAGAGACAAATTTGCTA+GGG + Chr4:1232391-1232410 None:intergenic 30.0%
!! GACATTAAAGATTTGTTGAG+TGG - Chr4:1233210-1233229 MsG0480018177.01.T01:intron 30.0%
!!! GACTTTTGTTTTACTTGCTA+TGG - Chr4:1232347-1232366 MsG0480018177.01.T01:intron 30.0%
AACCTTACAGAAAATCAGTC+AGG + Chr4:1231107-1231126 None:intergenic 35.0%
AAGCAACTAATCTACCTCAA+CGG + Chr4:1231370-1231389 None:intergenic 35.0%
AGTTTCCGATGAATTATCGA+TGG + Chr4:1233850-1233869 None:intergenic 35.0%
ATAGAACAAGTTGTCACTGT+TGG - Chr4:1231225-1231244 MsG0480018177.01.T01:intron 35.0%
ATTCGACCAGTATATTTCGA+AGG + Chr4:1232569-1232588 None:intergenic 35.0%
CAATCGTCAGATGAATCTAA+AGG - Chr4:1233634-1233653 MsG0480018177.01.T01:intron 35.0%
CAGTTTCCGATGAATTATCT+TGG + Chr4:1233878-1233897 None:intergenic 35.0%
CTACTCCATCGATAATTCAT+CGG - Chr4:1233842-1233861 MsG0480018177.01.T01:intron 35.0%
CTTAAAAGGTCCTAATTGCT+AGG - Chr4:1232144-1232163 MsG0480018177.01.T01:intron 35.0%
CTTAACATAAGCACACTCTA+AGG + Chr4:1233278-1233297 None:intergenic 35.0%
CTTAGAGTGTGCTTATGTTA+AGG - Chr4:1233276-1233295 MsG0480018177.01.T01:intron 35.0%
GCCTTCAAAACAAGTGAATA+TGG - Chr4:1231916-1231935 MsG0480018177.01.T01:intron 35.0%
TCGTTCCTCATAGATAAAGA+TGG + Chr4:1233946-1233965 None:intergenic 35.0%
TGACCACCAAGATAATTCAT+CGG - Chr4:1233869-1233888 MsG0480018177.01.T01:intron 35.0%
TTCGACCAGTATATTTCGAA+GGG + Chr4:1232568-1232587 None:intergenic 35.0%
TTTCCGATGAATTATCTTGG+TGG + Chr4:1233875-1233894 None:intergenic 35.0%
! AAGTTAGACTTTTCCTTGGT+GGG + Chr4:1232538-1232557 None:intergenic 35.0%
! ACTGCAGAGAAATTTTTCTG+CGG + Chr4:1231290-1231309 None:intergenic 35.0%
! AGATTAGCTAGAGATTTGCT+AGG + Chr4:1232443-1232462 None:intergenic 35.0%
! ATGACTATTTGGCTCATCAA+AGG - Chr4:1230795-1230814 MsG0480018177.01.T01:CDS 35.0%
! CCTTCTTTAAGAGGTAATGT+CGG + Chr4:1233560-1233579 None:intergenic 35.0%
! CTAGGGAATACCTATTGATT+TGG + Chr4:1232425-1232444 None:intergenic 35.0%
! GATTGAGAGACAAATTTGCT+AGG + Chr4:1232392-1232411 None:intergenic 35.0%
! GTTTAGCTTATACTTCACCA+AGG - Chr4:1232652-1232671 MsG0480018177.01.T01:intron 35.0%
! TCTAGCTAATCTTGTTTGCT+AGG - Chr4:1232451-1232470 MsG0480018177.01.T01:intron 35.0%
! TCTCAAGTTAGACTTTTCCT+TGG + Chr4:1232672-1232691 None:intergenic 35.0%
! TTTTGAATGCTCTTTTGCTG+CGG - Chr4:1232304-1232323 MsG0480018177.01.T01:intron 35.0%
!! AACCTGACTGATTTTCTGTA+AGG - Chr4:1231102-1231121 MsG0480018177.01.T01:intron 35.0%
!! AAGACATGGTTATTTTGCTG+CGG - Chr4:1232273-1232292 MsG0480018177.01.T01:intron 35.0%
!! GATTAGCTAGAGATTTGCTA+GGG + Chr4:1232442-1232461 None:intergenic 35.0%
!! GCTTGGTAATTCGTTTTCTT+TGG - Chr4:1231580-1231599 MsG0480018177.01.T01:intron 35.0%
!! TCAATCTAAGAGGTATCTAC+TGG - Chr4:1230955-1230974 MsG0480018177.01.T01:intron 35.0%
!! TTTCTGCTGTAGATTTGCTA+TGG - Chr4:1232192-1232211 MsG0480018177.01.T01:intron 35.0%
!!! GCCATATTCACTTGTTTTGA+AGG + Chr4:1231920-1231939 None:intergenic 35.0%
!!! TTTTACTTAGCTTTGCTCGT+TGG - Chr4:1231710-1231729 MsG0480018177.01.T01:intron 35.0%
AAATTCCCGTTACGTAGCAT+GGG + Chr4:1231949-1231968 None:intergenic 40.0%
AATAGTCATCACCATGTTGG+TGG + Chr4:1230784-1230803 None:intergenic 40.0%
AATTAGCTGCGAATTAGCTC+CGG - Chr4:1232474-1232493 MsG0480018177.01.T01:intron 40.0%
ACACCGAAAGAATCGAACAT+AGG + Chr4:1233302-1233321 None:intergenic 40.0%
ACCAGAAGCAGAATCAACAA+TGG + Chr4:1230825-1230844 None:intergenic 40.0%
AGAAAATTGGGGAGAGAGAA+TGG - Chr4:1234069-1234088 MsG0480018177.01.T01:CDS 40.0%
AGATCGGAATCAGAGAAGAA+CGG + Chr4:1233788-1233807 None:intergenic 40.0%
AGCAACTAATCTACCTCAAC+GGG + Chr4:1231369-1231388 None:intergenic 40.0%
AGCTGAATGCTTGAGAAACT+AGG + Chr4:1231452-1231471 None:intergenic 40.0%
AGCTTGAGACGGACATTAAT+TGG - Chr4:1232629-1232648 MsG0480018177.01.T01:intron 40.0%
AGGCCTATGTTCGATTCTTT+CGG - Chr4:1233296-1233315 MsG0480018177.01.T01:intron 40.0%
CATGCTACAGCCAAATCAAT+AGG - Chr4:1232412-1232431 MsG0480018177.01.T01:intron 40.0%
CCAAATAGTCATCACCATGT+TGG + Chr4:1230787-1230806 None:intergenic 40.0%
CCGACATTACCTCTTAAAGA+AGG - Chr4:1233557-1233576 MsG0480018177.01.T01:intron 40.0%
GAAAATGACTCTGACAACGA+CGG - Chr4:1234005-1234024 MsG0480018177.01.T01:CDS 40.0%
GAAAGTGAGTAGAGAGAAAC+AGG + Chr4:1232967-1232986 None:intergenic 40.0%
GAACAAACACAACATGCTTG+TGG + Chr4:1233188-1233207 None:intergenic 40.0%
GATCTGCCATGTATTAATGC+CGG - Chr4:1231318-1231337 MsG0480018177.01.T01:intron 40.0%
GATTTGTCTTCACTGAAGAG+AGG + Chr4:1230854-1230873 None:intergenic 40.0%
GCTTGAGACGGACATTAATT+GGG - Chr4:1232630-1232649 MsG0480018177.01.T01:intron 40.0%
GGAGAAGAAAGAGGAATTGA+CGG - Chr4:1234031-1234050 MsG0480018177.01.T01:CDS 40.0%
GTAGAATAGATCGTTGTCGT+CGG + Chr4:1233826-1233845 None:intergenic 40.0%
TCTCACATCGTGTTCAGTTT+GGG - Chr4:1232603-1232622 MsG0480018177.01.T01:intron 40.0%
TGAATTAAGTACCCCCTCTA+AGG - Chr4:1233104-1233123 MsG0480018177.01.T01:CDS 40.0%
TGCATATTTGATGTGGTGAG+CGG + Chr4:1233973-1233992 None:intergenic 40.0%
TGTTCATGTTTCTTCTCCCT+CGG - Chr4:1233381-1233400 MsG0480018177.01.T01:intron 40.0%
TTCTCACATCGTGTTCAGTT+TGG - Chr4:1232602-1232621 MsG0480018177.01.T01:intron 40.0%
TTGGGGAGAGAGAGAAAATT+GGG - Chr4:1234057-1234076 MsG0480018177.01.T01:CDS 40.0%
TTGGTCCCTTCGAAATATAC+TGG - Chr4:1232560-1232579 MsG0480018177.01.T01:intron 40.0%
! AAAGAGGAATTGACGGATCT+TGG - Chr4:1234038-1234057 MsG0480018177.01.T01:CDS 40.0%
! AAGAGGAATTGACGGATCTT+GGG - Chr4:1234039-1234058 MsG0480018177.01.T01:CDS 40.0%
! CAAGTTAGACTTTTCCTTGG+TGG + Chr4:1232539-1232558 None:intergenic 40.0%
! CCAACATGGTGATGACTATT+TGG - Chr4:1230784-1230803 MsG0480018177.01.T01:CDS 40.0%
! GGCTTTGGTGTAACATTGAA+GGG + Chr4:1231739-1231758 None:intergenic 40.0%
!! AGTGTTCATGGTTCTGATGT+AGG - Chr4:1232819-1232838 MsG0480018177.01.T01:intron 40.0%
!! AGTTTGGGATTAGCTTGAGA+CGG - Chr4:1232618-1232637 MsG0480018177.01.T01:intron 40.0%
!! ATGCTTGTGGAACTTGTTAG+AGG + Chr4:1233175-1233194 None:intergenic 40.0%
!! CTATTGATTTGGCTGTAGCA+TGG + Chr4:1232414-1232433 None:intergenic 40.0%
!! TGATTTGCTATGCCCATTTC+CGG + Chr4:1232496-1232515 None:intergenic 40.0%
!! TGCTTGTGGAACTTGTTAGA+GGG + Chr4:1233174-1233193 None:intergenic 40.0%
!!! CGTTTTGTTGTTGCACTGTT+TGG + Chr4:1232728-1232747 None:intergenic 40.0%
AAAGATGTTCCTTAGACCCG+AGG + Chr4:1233401-1233420 None:intergenic 45.0%
AAGATGTTCCTTAGACCCGA+GGG + Chr4:1233400-1233419 None:intergenic 45.0%
ATATACGAGGATGTGTTCGG+TGG + Chr4:1232996-1233015 None:intergenic 45.0%
ATATTTGATGTGGTGAGCGG+AGG + Chr4:1233970-1233989 None:intergenic 45.0%
CCACATCAAATATGCAGCAC+AGG - Chr4:1233977-1233996 MsG0480018177.01.T01:CDS 45.0%
CCGATCCATCTCTGATTCAT+CGG - Chr4:1233749-1233768 MsG0480018177.01.T01:intron 45.0%
CCGATGAATCAGAGATGGAT+CGG + Chr4:1233752-1233771 None:intergenic 45.0%
CCTGTGCTGCATATTTGATG+TGG + Chr4:1233980-1233999 None:intergenic 45.0%
CGATGAATCAGAGATGGATC+GGG + Chr4:1233751-1233770 None:intergenic 45.0%
CGTTTCCGATGAATCAGAGA+TGG + Chr4:1233757-1233776 None:intergenic 45.0%
CTGCCTAGACTTCTTCACAA+GGG + Chr4:1231784-1231803 None:intergenic 45.0%
CTTGGGGAGAGAGAGAAAAT+TGG - Chr4:1234056-1234075 MsG0480018177.01.T01:CDS 45.0%
GAAATTCCCGTTACGTAGCA+TGG + Chr4:1231950-1231969 None:intergenic 45.0%
GCAACTAATCTACCTCAACG+GGG + Chr4:1231368-1231387 None:intergenic 45.0%
GGCTTTCCGGCATTAATACA+TGG + Chr4:1231327-1231346 None:intergenic 45.0%
GTTCATGTTTCTTCTCCCTC+GGG - Chr4:1233382-1233401 MsG0480018177.01.T01:intron 45.0%
TCTGCCTAGACTTCTTCACA+AGG + Chr4:1231785-1231804 None:intergenic 45.0%
TGATCTCAGTTTCGCAACTG+AGG - Chr4:1230910-1230929 MsG0480018177.01.T01:CDS 45.0%
TGCGAATTAGCTCCGGAAAT+GGG - Chr4:1232481-1232500 MsG0480018177.01.T01:intron 45.0%
TGGGGAGAGAGAGAAAATTG+GGG - Chr4:1234058-1234077 MsG0480018177.01.T01:CDS 45.0%
TTCTTGTAGTGAACCCACCA+AGG - Chr4:1232522-1232541 MsG0480018177.01.T01:intron 45.0%
! AGAGGAATTGACGGATCTTG+GGG - Chr4:1234040-1234059 MsG0480018177.01.T01:CDS 45.0%
! GGGCTTTGGTGTAACATTGA+AGG + Chr4:1231740-1231759 None:intergenic 45.0%
!! GATGGAATCTGGAAGTGTCT+CGG + Chr4:1233928-1233947 None:intergenic 45.0%
!! GCCATTGTTGATTCTGCTTC+TGG - Chr4:1230821-1230840 MsG0480018177.01.T01:CDS 45.0%
AAGACCCTCATCTGCTCTTG+AGG + Chr4:1231810-1231829 None:intergenic 50.0%
ACCTACATTTGTCCCCGTTG+AGG - Chr4:1231353-1231372 MsG0480018177.01.T01:intron 50.0%
ACCTCAACGGGGACAAATGT+AGG + Chr4:1231357-1231376 None:intergenic 50.0%
AGAAGCCTCAAGAGCAGATG+AGG - Chr4:1231802-1231821 MsG0480018177.01.T01:intron 50.0%
AGAGCAGATGAGGGTCTTGA+CGG - Chr4:1231812-1231831 MsG0480018177.01.T01:intron 50.0%
CCTAGACTTCTTCACAAGGG+AGG + Chr4:1231781-1231800 None:intergenic 50.0%
CCTCCCTTGTGAAGAAGTCT+AGG - Chr4:1231778-1231797 MsG0480018177.01.T01:intron 50.0%
CTGCGAATTAGCTCCGGAAA+TGG - Chr4:1232480-1232499 MsG0480018177.01.T01:intron 50.0%
GAAGCCTCAAGAGCAGATGA+GGG - Chr4:1231803-1231822 MsG0480018177.01.T01:intron 50.0%
GGGACAAATGTAGGTCGCTT+TGG + Chr4:1231348-1231367 None:intergenic 50.0%
TCTGAGCCCATGCTACGTAA+CGG - Chr4:1231940-1231959 MsG0480018177.01.T01:intron 50.0%
TCTTCACAAGGGAGGACCTT+TGG + Chr4:1231773-1231792 None:intergenic 50.0%
TTTCTTCTCCCTCGGGTCTA+AGG - Chr4:1233389-1233408 MsG0480018177.01.T01:intron 50.0%
!! TGTAGGTCGCTTTGGCTTTC+CGG + Chr4:1231340-1231359 None:intergenic 50.0%
CGACGGTGTGGAGAAGAAAG+AGG - Chr4:1234022-1234041 MsG0480018177.01.T01:CDS 55.0%
CTGAGCCCATGCTACGTAAC+GGG - Chr4:1231941-1231960 MsG0480018177.01.T01:intron 55.0%
TGACTCTGACAACGACGGTG+TGG - Chr4:1234010-1234029 MsG0480018177.01.T01:CDS 55.0%
! GAAGTGTCTCGGTGGCCATT+CGG + Chr4:1233917-1233936 None:intergenic 55.0%
!! AAGGGAGGACCTTTGGAGTG+TGG + Chr4:1231766-1231785 None:intergenic 55.0%
!! AGGGAGGACCTTTGGAGTGT+GGG + Chr4:1231765-1231784 None:intergenic 55.0%
!! GGAATCTGGAAGTGTCTCGG+TGG + Chr4:1233925-1233944 None:intergenic 55.0%
AAGCCCTGCCCACACTCCAA+AGG - Chr4:1231754-1231773 MsG0480018177.01.T01:intron 60.0%
TCACCAGAAACGCCGCCGAA+TGG - Chr4:1233899-1233918 MsG0480018177.01.T01:intron 60.0%
TTGGAGTGTGGGCAGGGCTT+TGG + Chr4:1231754-1231773 None:intergenic 60.0%
! TGGAGTGGGCTCCACCAACA+TGG - Chr4:1230770-1230789 MsG0480018177.01.T01:CDS 60.0%
!! AGGACCTTTGGAGTGTGGGC+AGG + Chr4:1231761-1231780 None:intergenic 60.0%
!! GGACCTTTGGAGTGTGGGCA+GGG + Chr4:1231760-1231779 None:intergenic 60.0%
ACCGTGGCCCAGACTGGAGT+GGG - Chr4:1230756-1230775 MsG0480018177.01.T01:CDS 65.0%
GGTGGAGCCCACTCCAGTCT+GGG + Chr4:1230766-1230785 None:intergenic 65.0%
GTGTCTCGGTGGCCATTCGG+CGG + Chr4:1233914-1233933 None:intergenic 65.0%
TGGCCATTCGGCGGCGTTTC+TGG + Chr4:1233905-1233924 None:intergenic 65.0%
TGGTGGAGCCCACTCCAGTC+TGG + Chr4:1230767-1230786 None:intergenic 65.0%
GACCGTGGCCCAGACTGGAG+TGG - Chr4:1230755-1230774 MsG0480018177.01.T01:CDS 70.0%
GCCCACTCCAGTCTGGGCCA+CGG + Chr4:1230760-1230779 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 1230752 1234109 1230752 ID=MsG0480018177.01;Name=MsG0480018177.01
Chr4 mRNA 1230752 1234109 1230752 ID=MsG0480018177.01.T01;Parent=MsG0480018177.01;Name=MsG0480018177.01.T01;_AED=0.67;_eAED=0.87;_QI=0|0|0.33|0.66|1|1|3|0|166
Chr4 exon 1233930 1234109 1233930 ID=MsG0480018177.01.T01:exon:850;Parent=MsG0480018177.01.T01
Chr4 exon 1233025 1233141 1233025 ID=MsG0480018177.01.T01:exon:849;Parent=MsG0480018177.01.T01
Chr4 exon 1230752 1230955 1230752 ID=MsG0480018177.01.T01:exon:848;Parent=MsG0480018177.01.T01
Chr4 CDS 1233930 1234109 1233930 ID=MsG0480018177.01.T01:cds;Parent=MsG0480018177.01.T01
Chr4 CDS 1233025 1233141 1233025 ID=MsG0480018177.01.T01:cds;Parent=MsG0480018177.01.T01
Chr4 CDS 1230752 1230955 1230752 ID=MsG0480018177.01.T01:cds;Parent=MsG0480018177.01.T01
Gene Sequence

>MsG0480018177.01.T01

ATGGACCGTGGCCCAGACTGGAGTGGGCTCCACCAACATGGTGATGACTATTTGGCTCATCAAAGGTTAGCCATTGTTGATTCTGCTTCTGGTGATCAACCTCTCTTCAGTGAAGACAAATCAATTATTGTTCTGGGAAGTTCTGAATTGTTAATGTATGATCTCAGTTTCGCAACTGAGCTTTGCTCGTTGGTATCCCTTCAATGTTACACCAAAGCCCTGCCCACACTCCAAAGGTCCTCCCTTGTGAAGAAGTCTAGGCAGAAGCCTCAAGAGCAGATGAGGGTCTTGACGGATAAACGCCGCCGAATGGCCACCGAGACACTTCCAGATTCCATCTTTATCTATGAGGAACGATCCTCCGCTCACCACATCAAATATGCAGCACAGGTTTATGAAAATGACTCTGACAACGACGGTGTGGAGAAGAAAGAGGAATTGACGGATCTTGGGGAGAGAGAGAAAATTGGGGAGAGAGAATGGAAAAGTAGTAATGGGTAA
Protein sequence

>MsG0480018177.01.T01

MDRGPDWSGLHQHGDDYLAHQRLAIVDSASGDQPLFSEDKSIIVLGSSELLMYDLSFATELCSLVSLQCYTKALPTLQRSSLVKKSRQKPQEQMRVLTDKRRRMATETLPDSIFIYEERSSAHHIKYAAQVYENDSDNDGVEKKEELTDLGEREKIGEREWKSSNG*