AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018180.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018180.01.T01 MTR_3g464580 83.333 48 8 0 3 50 31 78 1.79e-21 90.1
MsG0480018180.01.T01 MTR_3g464580 83.333 48 8 0 3 50 31 78 1.82e-21 90.5
MsG0480018180.01.T01 MTR_3g464580 83.333 48 8 0 3 50 31 78 1.95e-21 90.1
MsG0480018180.01.T01 MTR_5g071360 79.167 48 10 0 3 50 31 78 3.21e-20 87.0
MsG0480018180.01.T01 MTR_5g071360 79.167 48 10 0 3 50 31 78 3.49e-20 86.7
MsG0480018180.01.T01 MTR_3g087220 68.750 48 15 0 3 50 31 78 3.18e-16 75.5
MsG0480018180.01.T01 MTR_5g087870 62.500 64 21 1 36 99 404 464 6.03e-15 72.0
MsG0480018180.01.T01 MTR_5g087870 62.500 64 21 1 36 99 404 464 6.62e-15 71.6
MsG0480018180.01.T01 MTR_3g078647 59.375 64 23 1 36 99 379 439 1.30e-14 70.9
MsG0480018180.01.T01 MTR_5g087890 46.429 84 34 2 16 99 342 414 6.75e-12 63.2
MsG0480018180.01.T01 MTR_3g083300 72.500 40 11 0 60 99 382 421 5.89e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018180.01.T01 AT3G47340 70.833 48 14 0 3 50 31 78 6.07e-19 83.2
MsG0480018180.01.T01 AT3G47340 70.833 48 14 0 3 50 31 78 7.75e-19 83.2
MsG0480018180.01.T01 AT3G47340 70.833 48 14 0 3 50 31 78 8.03e-19 82.8
MsG0480018180.01.T01 AT5G10240 66.667 48 16 0 3 50 31 78 3.64e-15 72.4
MsG0480018180.01.T01 AT5G10240 66.667 48 16 0 3 50 31 78 3.71e-15 72.4
MsG0480018180.01.T01 AT5G65010 62.500 48 18 0 3 50 31 78 1.21e-14 70.9
MsG0480018180.01.T01 AT5G65010 62.500 48 18 0 3 50 31 78 1.21e-14 70.9
MsG0480018180.01.T01 AT2G27040 53.968 63 26 1 36 98 381 440 1.34e-11 62.4
MsG0480018180.01.T01 AT2G27040 53.968 63 26 1 36 98 381 440 1.34e-11 62.4

Find 60 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCATTGTTGATTCTGCTTC+TGG 0.237345 4:-1258222 MsG0480018180.01.T01:CDS
AGACAAATCAATTATTGTTC+TGG 0.260232 4:-1258178 MsG0480018180.01.T01:CDS
CCAACATGGTGATGACTATT+TGG 0.278820 4:-1258259 MsG0480018180.01.T01:CDS
TGGTGGAGCCCACTCCAGTC+TGG 0.295762 4:+1258279 None:intergenic
TTGGGGAGAGAGAGAAAATT+GGG 0.315927 4:-1254985 MsG0480018180.01.T01:CDS
TTGGAGTGTGGGCAGGGCTT+TGG 0.335047 4:+1257292 None:intergenic
AAAGAGGAATTGACGGATCT+TGG 0.339190 4:-1255004 MsG0480018180.01.T01:CDS
AGGACCTTTGGAGTGTGGGC+AGG 0.341217 4:+1257285 None:intergenic
CTTGGGGAGAGAGAGAAAAT+TGG 0.341385 4:-1254986 MsG0480018180.01.T01:CDS
TCTTCACAAGGGAGGACCTT+TGG 0.355434 4:+1257273 None:intergenic
GACAAATCAATTATTGTTCT+GGG 0.365959 4:-1258177 MsG0480018180.01.T01:CDS
AGAAAATTGGGGAGAGAGAA+TGG 0.370972 4:-1254973 MsG0480018180.01.T01:CDS
AAGAGGAATTGACGGATCTT+GGG 0.371737 4:-1255003 MsG0480018180.01.T01:CDS
GGACCTTTGGAGTGTGGGCA+GGG 0.402108 4:+1257286 None:intergenic
TCATAGATAAAGATGGAATC+TGG 0.410910 4:+1255106 None:intergenic
GAGAATGGAAAAGTAGTAAT+GGG 0.419805 4:-1254958 MsG0480018180.01.T01:CDS
GGTGGAGCCCACTCCAGTCT+GGG 0.459596 4:+1258280 None:intergenic
AGATTCCATCTTTATCTATG+AGG 0.461675 4:-1255104 MsG0480018180.01.T01:CDS
GAAGTGTCTCGGTGGCCATT+CGG 0.464702 4:+1255128 None:intergenic
AGAGAATGGAAAAGTAGTAA+TGG 0.478460 4:-1254959 MsG0480018180.01.T01:CDS
GGGCTTTGGTGTAACATTGA+AGG 0.483853 4:+1257306 None:intergenic
GAATGGACCGTGGCCCAGAC+TGG 0.486813 4:-1258293 MsG0480018180.01.T01:CDS
AGAAGCCTCAAGAGCAGATG+AGG 0.488444 4:-1257241 MsG0480018180.01.T01:CDS
GGAGAAGAAAGAGGAATTGA+CGG 0.503111 4:-1255011 MsG0480018180.01.T01:CDS
CCTCCCTTGTGAAGAAGTCT+AGG 0.515464 4:-1257265 MsG0480018180.01.T01:CDS
GCCCACTCCAGTCTGGGCCA+CGG 0.525119 4:+1258286 None:intergenic
GGCTTTGGTGTAACATTGAA+GGG 0.530378 4:+1257307 None:intergenic
ACCGTGGCCCAGACTGGAGT+GGG 0.540984 4:-1258287 MsG0480018180.01.T01:CDS
AAGACCCTCATCTGCTCTTG+AGG 0.541011 4:+1257236 None:intergenic
AATAGTCATCACCATGTTGG+TGG 0.545301 4:+1258262 None:intergenic
TCGTTCCTCATAGATAAAGA+TGG 0.546006 4:+1255099 None:intergenic
ATGACTATTTGGCTCATCAA+AGG 0.546458 4:-1258248 MsG0480018180.01.T01:CDS
AGGGAGGACCTTTGGAGTGT+GGG 0.550613 4:+1257281 None:intergenic
CCACATCAAATATGCAGCAC+AGG 0.553723 4:-1255065 MsG0480018180.01.T01:CDS
AGAGCAGATGAGGGTCTTGA+CGG 0.560421 4:-1257231 MsG0480018180.01.T01:CDS
GACCGTGGCCCAGACTGGAG+TGG 0.571259 4:-1258288 MsG0480018180.01.T01:CDS
AAGGGAGGACCTTTGGAGTG+TGG 0.573278 4:+1257280 None:intergenic
TCACCAGAAACGCCGCCGAA+TGG 0.575604 4:-1255143 MsG0480018180.01.T01:intron
AGAGGAATTGACGGATCTTG+GGG 0.581809 4:-1255002 MsG0480018180.01.T01:CDS
TGGGGAGAGAGAGAAAATTG+GGG 0.586510 4:-1254984 MsG0480018180.01.T01:CDS
AAGCCCTGCCCACACTCCAA+AGG 0.597838 4:-1257289 MsG0480018180.01.T01:CDS
CGACGGTGTGGAGAAGAAAG+AGG 0.598705 4:-1255020 MsG0480018180.01.T01:CDS
GATTTGTCTTCACTGAAGAG+AGG 0.600995 4:+1258192 None:intergenic
GTGTCTCGGTGGCCATTCGG+CGG 0.602622 4:+1255131 None:intergenic
CCAAATAGTCATCACCATGT+TGG 0.608389 4:+1258259 None:intergenic
GATGGAATCTGGAAGTGTCT+CGG 0.609927 4:+1255117 None:intergenic
GAAGCCTCAAGAGCAGATGA+GGG 0.640454 4:-1257240 MsG0480018180.01.T01:CDS
TGGAGTGGGCTCCACCAACA+TGG 0.641715 4:-1258273 MsG0480018180.01.T01:CDS
TGATCTCAGTTTCGCAACTG+AGG 0.642571 4:-1258133 MsG0480018180.01.T01:intron
TGACTCTGACAACGACGGTG+TGG 0.648622 4:-1255032 MsG0480018180.01.T01:CDS
TGCATATTTGATGTGGTGAG+CGG 0.650305 4:+1255072 None:intergenic
ACCAGAAGCAGAATCAACAA+TGG 0.683453 4:+1258221 None:intergenic
CCTAGACTTCTTCACAAGGG+AGG 0.685125 4:+1257265 None:intergenic
TCTGCCTAGACTTCTTCACA+AGG 0.686387 4:+1257261 None:intergenic
ATATTTGATGTGGTGAGCGG+AGG 0.692738 4:+1255075 None:intergenic
TGCTGCCGTTGAATGGACCG+TGG 0.694251 4:-1258303 None:intergenic
CTGCCTAGACTTCTTCACAA+GGG 0.694383 4:+1257262 None:intergenic
GAAAATGACTCTGACAACGA+CGG 0.699509 4:-1255037 MsG0480018180.01.T01:CDS
CCTGTGCTGCATATTTGATG+TGG 0.706971 4:+1255065 None:intergenic
GGAATCTGGAAGTGTCTCGG+TGG 0.718363 4:+1255120 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAAAGAAAAAAAAAAC+AGG + Chr4:1256181-1256200 None:intergenic 15.0%
!!! AATAGTATTGATAACAAATT+AGG + Chr4:1256256-1256275 None:intergenic 15.0%
!!! TGTTTTTTTTAAAGCTTAAA+AGG - Chr4:1256335-1256354 MsG0480018180.01.T01:intron 15.0%
!!! TTTTTCTTTTTTTCTTTTGA+GGG - Chr4:1256187-1256206 MsG0480018180.01.T01:intron 15.0%
!!! TTTTTTCTTTTTTTCTTTTG+AGG - Chr4:1256186-1256205 MsG0480018180.01.T01:intron 15.0%
!! AATGTTCCTTATTAATTGAT+TGG - Chr4:1256300-1256319 MsG0480018180.01.T01:intron 20.0%
!! ACTAACTAATACACATTATT+AGG + Chr4:1255615-1255634 None:intergenic 20.0%
!! ATATTCAACTATAATATACG+AGG + Chr4:1257214-1257233 None:intergenic 20.0%
!! GTTCCTATTTAATGTATATA+AGG - Chr4:1255759-1255778 MsG0480018180.01.T01:intron 20.0%
!! TCAGCTATTATGTATTATTT+AGG - Chr4:1255669-1255688 MsG0480018180.01.T01:intron 20.0%
!! TTGATTATGAATAAGGATTT+AGG - Chr4:1255243-1255262 MsG0480018180.01.T01:intron 20.0%
!!! AAGTTTGTTAATTCATTCAT+TGG + Chr4:1255811-1255830 None:intergenic 20.0%
!!! AGTAGATTTTATGTATTGAT+TGG + Chr4:1257107-1257126 None:intergenic 20.0%
!!! CTATTTTACTTTACAATCTA+TGG + Chr4:1255412-1255431 None:intergenic 20.0%
! AAAATTGCTATGATAAGACA+TGG - Chr4:1256464-1256483 MsG0480018180.01.T01:intron 25.0%
! AACTTGAGACAATCATTAAT+TGG - Chr4:1256888-1256907 MsG0480018180.01.T01:intron 25.0%
! AAGGAAAAATATAACACTTC+AGG - Chr4:1257328-1257347 MsG0480018180.01.T01:intron 25.0%
! AAGTAATTCAACATTAAGAG+AGG + Chr4:1256225-1256244 None:intergenic 25.0%
! ACAAGTAAAACAAAAAGGAA+AGG + Chr4:1257664-1257683 None:intergenic 25.0%
! ACTTGAGACAATCATTAATT+GGG - Chr4:1256889-1256908 MsG0480018180.01.T01:intron 25.0%
! AGACAAATCAATTATTGTTC+TGG - Chr4:1255068-1255087 MsG0480018180.01.T01:CDS 25.0%
! ATTAAATTCATCAACTCAAG+TGG + Chr4:1257445-1257464 None:intergenic 25.0%
! CAATCTAATATCAATCTAAG+AGG - Chr4:1255148-1255167 MsG0480018180.01.T01:intron 25.0%
! CTCTTAGATTGATATTAGAT+TGG + Chr4:1255150-1255169 None:intergenic 25.0%
! CTTTACTGTTAAGATTAACA+AGG - Chr4:1255719-1255738 MsG0480018180.01.T01:intron 25.0%
! GACAAATCAATTATTGTTCT+GGG - Chr4:1255069-1255088 MsG0480018180.01.T01:CDS 25.0%
! GCACCTTATATACATTAAAT+AGG + Chr4:1255765-1255784 None:intergenic 25.0%
! GGATTAACTAAATATTTGCT+AGG - Chr4:1256530-1256549 MsG0480018180.01.T01:intron 25.0%
! TGTGTGATTGATTATGAATA+AGG - Chr4:1255236-1255255 MsG0480018180.01.T01:intron 25.0%
! TTAAATTCATCAACTCAAGT+GGG + Chr4:1257444-1257463 None:intergenic 25.0%
! TTGTCACAAGTAAAACAAAA+AGG + Chr4:1257669-1257688 None:intergenic 25.0%
! TTTAATGAATCCTAGCAATT+AGG + Chr4:1256362-1256381 None:intergenic 25.0%
!! AGGTAGTAGAGATTTTTAAA+TGG - Chr4:1255689-1255708 MsG0480018180.01.T01:intron 25.0%
!! AGTGTTATATTTTTCCTTAG+AGG + Chr4:1257326-1257345 None:intergenic 25.0%
!! ATATATTAGCTTAGTGTTCA+TGG - Chr4:1257012-1257031 MsG0480018180.01.T01:intron 25.0%
!! GTGTTATATTTTTCCTTAGA+GGG + Chr4:1257325-1257344 None:intergenic 25.0%
!! TGTTATATTTTTCCTTAGAG+GGG + Chr4:1257324-1257343 None:intergenic 25.0%
!! TTTAATGTATATAAGGTGCT+TGG - Chr4:1255766-1255785 MsG0480018180.01.T01:intron 25.0%
!!! AATATCATTTGCTGATTTAC+AGG - Chr4:1256097-1256116 MsG0480018180.01.T01:intron 25.0%
!!! ACTTGAGTTGATGAATTTAA+TGG - Chr4:1257444-1257463 MsG0480018180.01.T01:intron 25.0%
!!! TGAGTTATTTTGACTTCTTA+AGG - Chr4:1257536-1257555 MsG0480018180.01.T01:intron 25.0%
!!! TTTATTCACTTTTCTTGAAG+TGG + Chr4:1257717-1257736 None:intergenic 25.0%
AAGGAAAAGTCTAACTTGAT+TGG - Chr4:1256746-1256765 MsG0480018180.01.T01:intron 30.0%
AATCATCATCCTTCTTTAAG+AGG + Chr4:1257774-1257793 None:intergenic 30.0%
AGAGAATGGAAAAGTAGTAA+TGG - Chr4:1258287-1258306 MsG0480018180.01.T01:CDS 30.0%
AGATGTAGTGTGCTATAATA+GGG + Chr4:1256979-1256998 None:intergenic 30.0%
AGATTCCATCTTTATCTATG+AGG - Chr4:1258142-1258161 MsG0480018180.01.T01:CDS 30.0%
ATAATATACGAGGATGTGTT+CGG + Chr4:1257204-1257223 None:intergenic 30.0%
GACAATGAATTGAAGAAGAT+CGG + Chr4:1258008-1258027 None:intergenic 30.0%
GAGAATGGAAAAGTAGTAAT+GGG - Chr4:1258288-1258307 MsG0480018180.01.T01:CDS 30.0%
GAGTTGTTGAGTGATAATAA+TGG - Chr4:1257558-1257577 MsG0480018180.01.T01:intron 30.0%
GATAAGACTGAGAAGAATAT+TGG + Chr4:1257904-1257923 None:intergenic 30.0%
GGGAATTATTCTTCATTGTT+TGG + Chr4:1257935-1257954 None:intergenic 30.0%
GGGTACTTAATTCACTTTAA+AGG + Chr4:1257303-1257322 None:intergenic 30.0%
TAGATGTAGTGTGCTATAAT+AGG + Chr4:1256980-1256999 None:intergenic 30.0%
TCACTGCCAATCAATTAATA+AGG + Chr4:1256309-1256328 None:intergenic 30.0%
TCATAGATAAAGATGGAATC+TGG + Chr4:1258143-1258162 None:intergenic 30.0%
! AATCAAGTTAGACTTTTCCT+TGG + Chr4:1256747-1256766 None:intergenic 30.0%
! GTTATATTTTTCCTTAGAGG+GGG + Chr4:1257323-1257342 None:intergenic 30.0%
!! ATTAAAGATTTGTTGAGTGG+TGG - Chr4:1257418-1257437 MsG0480018180.01.T01:intron 30.0%
!! ATTGAGAGACAAATTTGCTA+GGG + Chr4:1256596-1256615 None:intergenic 30.0%
!! GACATTAAAGATTTGTTGAG+TGG - Chr4:1257415-1257434 MsG0480018180.01.T01:intron 30.0%
!!! GACTTTTGTTTTACTTGCTA+TGG - Chr4:1256552-1256571 MsG0480018180.01.T01:intron 30.0%
AACCTTACAGAAAATCAGTC+AGG + Chr4:1255310-1255329 None:intergenic 35.0%
AAGCAACTAATCTACCTCAA+CGG + Chr4:1255573-1255592 None:intergenic 35.0%
AGTTTCCGATGAATTATCGA+TGG + Chr4:1258054-1258073 None:intergenic 35.0%
ATAGAACAAGTTGTCACTGT+TGG - Chr4:1255428-1255447 MsG0480018180.01.T01:intron 35.0%
ATTCGACCAGTATATTTCGA+AGG + Chr4:1256774-1256793 None:intergenic 35.0%
CAATCGTCAGATGAATCTAA+AGG - Chr4:1257839-1257858 MsG0480018180.01.T01:intron 35.0%
CAGTTTCCGATGAATTATCT+TGG + Chr4:1258082-1258101 None:intergenic 35.0%
CTACTCCATCGATAATTCAT+CGG - Chr4:1258046-1258065 MsG0480018180.01.T01:intron 35.0%
CTTAAAAGGTCCTAATTGCT+AGG - Chr4:1256349-1256368 MsG0480018180.01.T01:intron 35.0%
CTTAACATAAGCACACTCTA+AGG + Chr4:1257483-1257502 None:intergenic 35.0%
CTTAGAGTGTGCTTATGTTA+AGG - Chr4:1257481-1257500 MsG0480018180.01.T01:intron 35.0%
GCCTTCAAAACAAGTGAATA+TGG - Chr4:1256119-1256138 MsG0480018180.01.T01:intron 35.0%
TCGTTCCTCATAGATAAAGA+TGG + Chr4:1258150-1258169 None:intergenic 35.0%
TGACCACCAAGATAATTCAT+CGG - Chr4:1258073-1258092 MsG0480018180.01.T01:intron 35.0%
TTCGACCAGTATATTTCGAA+GGG + Chr4:1256773-1256792 None:intergenic 35.0%
TTTCCGATGAATTATCTTGG+TGG + Chr4:1258079-1258098 None:intergenic 35.0%
! AAGTTAGACTTTTCCTTGGT+GGG + Chr4:1256743-1256762 None:intergenic 35.0%
! ACTGCAGAGAAATTTTTCTG+CGG + Chr4:1255493-1255512 None:intergenic 35.0%
! AGATTAGCTAGAGATTTGCT+AGG + Chr4:1256648-1256667 None:intergenic 35.0%
! ATGACTATTTGGCTCATCAA+AGG - Chr4:1254998-1255017 MsG0480018180.01.T01:CDS 35.0%
! CCTTCTTTAAGAGGTAATGT+CGG + Chr4:1257765-1257784 None:intergenic 35.0%
! CTAGGGAATACCTATTGATT+TGG + Chr4:1256630-1256649 None:intergenic 35.0%
! GATTGAGAGACAAATTTGCT+AGG + Chr4:1256597-1256616 None:intergenic 35.0%
! GTTTAGCTTATACTTCACCA+AGG - Chr4:1256857-1256876 MsG0480018180.01.T01:intron 35.0%
! TCTAGCTAATCTTGTTTGCT+AGG - Chr4:1256656-1256675 MsG0480018180.01.T01:intron 35.0%
! TCTCAAGTTAGACTTTTCCT+TGG + Chr4:1256877-1256896 None:intergenic 35.0%
! TTTTGAATGCTCTTTTGCTG+CGG - Chr4:1256509-1256528 MsG0480018180.01.T01:intron 35.0%
!! AACCTGACTGATTTTCTGTA+AGG - Chr4:1255305-1255324 MsG0480018180.01.T01:intron 35.0%
!! AAGACATGGTTATTTTGCTG+CGG - Chr4:1256478-1256497 MsG0480018180.01.T01:intron 35.0%
!! GATTAGCTAGAGATTTGCTA+GGG + Chr4:1256647-1256666 None:intergenic 35.0%
!! GCTTGGTAATTCGTTTTCTT+TGG - Chr4:1255783-1255802 MsG0480018180.01.T01:intron 35.0%
!! TCAATCTAAGAGGTATCTAC+TGG - Chr4:1255158-1255177 MsG0480018180.01.T01:intron 35.0%
!! TTTCTGCTGTAGATTTGCTA+TGG - Chr4:1256397-1256416 MsG0480018180.01.T01:intron 35.0%
!!! GCCATATTCACTTGTTTTGA+AGG + Chr4:1256123-1256142 None:intergenic 35.0%
!!! TTTTACTTAGCTTTGCTCGT+TGG - Chr4:1255913-1255932 MsG0480018180.01.T01:intron 35.0%
AAATTCCCGTTACGTAGCAT+GGG + Chr4:1256152-1256171 None:intergenic 40.0%
AATAGTCATCACCATGTTGG+TGG + Chr4:1254987-1255006 None:intergenic 40.0%
AATTAGCTGCGAATTAGCTC+CGG - Chr4:1256679-1256698 MsG0480018180.01.T01:intron 40.0%
ACACCGAAAGAATCGAACAT+AGG + Chr4:1257507-1257526 None:intergenic 40.0%
ACCAGAAGCAGAATCAACAA+TGG + Chr4:1255028-1255047 None:intergenic 40.0%
AGAAAATTGGGGAGAGAGAA+TGG - Chr4:1258273-1258292 MsG0480018180.01.T01:CDS 40.0%
AGATCGGAATCAGAGAAGAA+CGG + Chr4:1257992-1258011 None:intergenic 40.0%
AGCAACTAATCTACCTCAAC+GGG + Chr4:1255572-1255591 None:intergenic 40.0%
AGCTGAATGCTTGAGAAACT+AGG + Chr4:1255655-1255674 None:intergenic 40.0%
AGCTTGAGACGGACATTAAT+TGG - Chr4:1256834-1256853 MsG0480018180.01.T01:intron 40.0%
AGGCCTATGTTCGATTCTTT+CGG - Chr4:1257501-1257520 MsG0480018180.01.T01:intron 40.0%
CATGCTACAGCCAAATCAAT+AGG - Chr4:1256617-1256636 MsG0480018180.01.T01:intron 40.0%
CCAAATAGTCATCACCATGT+TGG + Chr4:1254990-1255009 None:intergenic 40.0%
CCGACATTACCTCTTAAAGA+AGG - Chr4:1257762-1257781 MsG0480018180.01.T01:intron 40.0%
GAAAATGACTCTGACAACGA+CGG - Chr4:1258209-1258228 MsG0480018180.01.T01:CDS 40.0%
GAAAGTGAGTAGAGAGAAAC+AGG + Chr4:1257172-1257191 None:intergenic 40.0%
GAACAAACACAACATGCTTG+TGG + Chr4:1257393-1257412 None:intergenic 40.0%
GATCTGCCATGTATTAATGC+CGG - Chr4:1255521-1255540 MsG0480018180.01.T01:intron 40.0%
GATTTGTCTTCACTGAAGAG+AGG + Chr4:1255057-1255076 None:intergenic 40.0%
GCTTGAGACGGACATTAATT+GGG - Chr4:1256835-1256854 MsG0480018180.01.T01:intron 40.0%
GGAGAAGAAAGAGGAATTGA+CGG - Chr4:1258235-1258254 MsG0480018180.01.T01:CDS 40.0%
GTAGAATAGATCGTTGTCGT+CGG + Chr4:1258030-1258049 None:intergenic 40.0%
TCTCACATCGTGTTCAGTTT+GGG - Chr4:1256808-1256827 MsG0480018180.01.T01:intron 40.0%
TGAATTAAGTACCCCCTCTA+AGG - Chr4:1257309-1257328 MsG0480018180.01.T01:CDS 40.0%
TGCATATTTGATGTGGTGAG+CGG + Chr4:1258177-1258196 None:intergenic 40.0%
TGTTCATGTTTCTTCTCCCT+CGG - Chr4:1257586-1257605 MsG0480018180.01.T01:intron 40.0%
TTCTCACATCGTGTTCAGTT+TGG - Chr4:1256807-1256826 MsG0480018180.01.T01:intron 40.0%
TTGGGGAGAGAGAGAAAATT+GGG - Chr4:1258261-1258280 MsG0480018180.01.T01:CDS 40.0%
TTGGTCCCTTCGAAATATAC+TGG - Chr4:1256765-1256784 MsG0480018180.01.T01:intron 40.0%
! AAAGAGGAATTGACGGATCT+TGG - Chr4:1258242-1258261 MsG0480018180.01.T01:CDS 40.0%
! AAGAGGAATTGACGGATCTT+GGG - Chr4:1258243-1258262 MsG0480018180.01.T01:CDS 40.0%
! CAAGTTAGACTTTTCCTTGG+TGG + Chr4:1256744-1256763 None:intergenic 40.0%
! CCAACATGGTGATGACTATT+TGG - Chr4:1254987-1255006 MsG0480018180.01.T01:CDS 40.0%
! GGCTTTGGTGTAACATTGAA+GGG + Chr4:1255942-1255961 None:intergenic 40.0%
!! AGTGTTCATGGTTCTGATGT+AGG - Chr4:1257024-1257043 MsG0480018180.01.T01:intron 40.0%
!! AGTTTGGGATTAGCTTGAGA+CGG - Chr4:1256823-1256842 MsG0480018180.01.T01:intron 40.0%
!! ATGCTTGTGGAACTTGTTAG+AGG + Chr4:1257380-1257399 None:intergenic 40.0%
!! CTATTGATTTGGCTGTAGCA+TGG + Chr4:1256619-1256638 None:intergenic 40.0%
!! TGATTTGCTATGCCCATTTC+CGG + Chr4:1256701-1256720 None:intergenic 40.0%
!! TGCTTGTGGAACTTGTTAGA+GGG + Chr4:1257379-1257398 None:intergenic 40.0%
!!! CGTTTTGTTGTTGCACTGTT+TGG + Chr4:1256933-1256952 None:intergenic 40.0%
AAAGATGTTCCTTAGACCCG+AGG + Chr4:1257606-1257625 None:intergenic 45.0%
AAGATGTTCCTTAGACCCGA+GGG + Chr4:1257605-1257624 None:intergenic 45.0%
ATATACGAGGATGTGTTCGG+TGG + Chr4:1257201-1257220 None:intergenic 45.0%
ATATTTGATGTGGTGAGCGG+AGG + Chr4:1258174-1258193 None:intergenic 45.0%
CCACATCAAATATGCAGCAC+AGG - Chr4:1258181-1258200 MsG0480018180.01.T01:CDS 45.0%
CCGATCCATCTCTGATTCAT+CGG - Chr4:1257953-1257972 MsG0480018180.01.T01:intron 45.0%
CCGATGAATCAGAGATGGAT+CGG + Chr4:1257956-1257975 None:intergenic 45.0%
CCTGTGCTGCATATTTGATG+TGG + Chr4:1258184-1258203 None:intergenic 45.0%
CGATGAATCAGAGATGGATC+GGG + Chr4:1257955-1257974 None:intergenic 45.0%
CGTTTCCGATGAATCAGAGA+TGG + Chr4:1257961-1257980 None:intergenic 45.0%
CTGCCTAGACTTCTTCACAA+GGG + Chr4:1255987-1256006 None:intergenic 45.0%
CTTGGGGAGAGAGAGAAAAT+TGG - Chr4:1258260-1258279 MsG0480018180.01.T01:CDS 45.0%
GAAATTCCCGTTACGTAGCA+TGG + Chr4:1256153-1256172 None:intergenic 45.0%
GCAACTAATCTACCTCAACG+GGG + Chr4:1255571-1255590 None:intergenic 45.0%
GGCTTTCCGGCATTAATACA+TGG + Chr4:1255530-1255549 None:intergenic 45.0%
GTTCATGTTTCTTCTCCCTC+GGG - Chr4:1257587-1257606 MsG0480018180.01.T01:intron 45.0%
TCTGCCTAGACTTCTTCACA+AGG + Chr4:1255988-1256007 None:intergenic 45.0%
TGATCTCAGTTTCGCAACTG+AGG - Chr4:1255113-1255132 MsG0480018180.01.T01:CDS 45.0%
TGCGAATTAGCTCCGGAAAT+GGG - Chr4:1256686-1256705 MsG0480018180.01.T01:intron 45.0%
TGGGGAGAGAGAGAAAATTG+GGG - Chr4:1258262-1258281 MsG0480018180.01.T01:CDS 45.0%
TTCTTGTAGTGAACCCACCA+AGG - Chr4:1256727-1256746 MsG0480018180.01.T01:intron 45.0%
! AGAGGAATTGACGGATCTTG+GGG - Chr4:1258244-1258263 MsG0480018180.01.T01:CDS 45.0%
! GGGCTTTGGTGTAACATTGA+AGG + Chr4:1255943-1255962 None:intergenic 45.0%
!! GATGGAATCTGGAAGTGTCT+CGG + Chr4:1258132-1258151 None:intergenic 45.0%
!! GCCATTGTTGATTCTGCTTC+TGG - Chr4:1255024-1255043 MsG0480018180.01.T01:CDS 45.0%
AAGACCCTCATCTGCTCTTG+AGG + Chr4:1256013-1256032 None:intergenic 50.0%
ACCTACATTTGTCCCCGTTG+AGG - Chr4:1255556-1255575 MsG0480018180.01.T01:intron 50.0%
ACCTCAACGGGGACAAATGT+AGG + Chr4:1255560-1255579 None:intergenic 50.0%
AGAAGCCTCAAGAGCAGATG+AGG - Chr4:1256005-1256024 MsG0480018180.01.T01:intron 50.0%
AGAGCAGATGAGGGTCTTGA+CGG - Chr4:1256015-1256034 MsG0480018180.01.T01:intron 50.0%
CCTAGACTTCTTCACAAGGG+AGG + Chr4:1255984-1256003 None:intergenic 50.0%
CCTCCCTTGTGAAGAAGTCT+AGG - Chr4:1255981-1256000 MsG0480018180.01.T01:intron 50.0%
CTGCGAATTAGCTCCGGAAA+TGG - Chr4:1256685-1256704 MsG0480018180.01.T01:intron 50.0%
GAAGCCTCAAGAGCAGATGA+GGG - Chr4:1256006-1256025 MsG0480018180.01.T01:intron 50.0%
GGGACAAATGTAGGTCGCTT+TGG + Chr4:1255551-1255570 None:intergenic 50.0%
TCTGAGCCCATGCTACGTAA+CGG - Chr4:1256143-1256162 MsG0480018180.01.T01:intron 50.0%
TCTTCACAAGGGAGGACCTT+TGG + Chr4:1255976-1255995 None:intergenic 50.0%
TTTCTTCTCCCTCGGGTCTA+AGG - Chr4:1257594-1257613 MsG0480018180.01.T01:intron 50.0%
!! TGTAGGTCGCTTTGGCTTTC+CGG + Chr4:1255543-1255562 None:intergenic 50.0%
CGACGGTGTGGAGAAGAAAG+AGG - Chr4:1258226-1258245 MsG0480018180.01.T01:CDS 55.0%
CTGAGCCCATGCTACGTAAC+GGG - Chr4:1256144-1256163 MsG0480018180.01.T01:intron 55.0%
TGACTCTGACAACGACGGTG+TGG - Chr4:1258214-1258233 MsG0480018180.01.T01:CDS 55.0%
! GAAGTGTCTCGGTGGCCATT+CGG + Chr4:1258121-1258140 None:intergenic 55.0%
!! AAGGGAGGACCTTTGGAGTG+TGG + Chr4:1255969-1255988 None:intergenic 55.0%
!! AGGGAGGACCTTTGGAGTGT+GGG + Chr4:1255968-1255987 None:intergenic 55.0%
!! GGAATCTGGAAGTGTCTCGG+TGG + Chr4:1258129-1258148 None:intergenic 55.0%
AAGCCCTGCCCACACTCCAA+AGG - Chr4:1255957-1255976 MsG0480018180.01.T01:intron 60.0%
TCACCAGAAACGCCGCCGAA+TGG - Chr4:1258103-1258122 MsG0480018180.01.T01:intron 60.0%
TTGGAGTGTGGGCAGGGCTT+TGG + Chr4:1255957-1255976 None:intergenic 60.0%
! TGGAGTGGGCTCCACCAACA+TGG - Chr4:1254973-1254992 MsG0480018180.01.T01:CDS 60.0%
!! AGGACCTTTGGAGTGTGGGC+AGG + Chr4:1255964-1255983 None:intergenic 60.0%
!! GGACCTTTGGAGTGTGGGCA+GGG + Chr4:1255963-1255982 None:intergenic 60.0%
ACCGTGGCCCAGACTGGAGT+GGG - Chr4:1254959-1254978 MsG0480018180.01.T01:CDS 65.0%
GGTGGAGCCCACTCCAGTCT+GGG + Chr4:1254969-1254988 None:intergenic 65.0%
GTGTCTCGGTGGCCATTCGG+CGG + Chr4:1258118-1258137 None:intergenic 65.0%
TGGCCATTCGGCGGCGTTTC+TGG + Chr4:1258109-1258128 None:intergenic 65.0%
TGGTGGAGCCCACTCCAGTC+TGG + Chr4:1254970-1254989 None:intergenic 65.0%
GACCGTGGCCCAGACTGGAG+TGG - Chr4:1254958-1254977 MsG0480018180.01.T01:CDS 70.0%
GCCCACTCCAGTCTGGGCCA+CGG + Chr4:1254963-1254982 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 1254955 1258313 1254955 ID=MsG0480018180.01;Name=MsG0480018180.01
Chr4 mRNA 1254955 1258313 1254955 ID=MsG0480018180.01.T01;Parent=MsG0480018180.01;Name=MsG0480018180.01.T01;_AED=0.67;_eAED=0.87;_QI=0|0|0.33|0.66|1|1|3|0|166
Chr4 exon 1258134 1258313 1258134 ID=MsG0480018180.01.T01:exon:856;Parent=MsG0480018180.01.T01
Chr4 exon 1257229 1257345 1257229 ID=MsG0480018180.01.T01:exon:855;Parent=MsG0480018180.01.T01
Chr4 exon 1254955 1255158 1254955 ID=MsG0480018180.01.T01:exon:854;Parent=MsG0480018180.01.T01
Chr4 CDS 1258134 1258313 1258134 ID=MsG0480018180.01.T01:cds;Parent=MsG0480018180.01.T01
Chr4 CDS 1257229 1257345 1257229 ID=MsG0480018180.01.T01:cds;Parent=MsG0480018180.01.T01
Chr4 CDS 1254955 1255158 1254955 ID=MsG0480018180.01.T01:cds;Parent=MsG0480018180.01.T01
Gene Sequence

>MsG0480018180.01.T01

ATGGACCGTGGCCCAGACTGGAGTGGGCTCCACCAACATGGTGATGACTATTTGGCTCATCAAAGGTTAGCCATTGTTGATTCTGCTTCTGGTGATCAACCTCTCTTCAGTGAAGACAAATCAATTATTGTTCTGGGAAGTTCTGAATTGTTAATGTATGATCTCAGTTTCGCAACTGAGCTTTGCTCGTTGGTATCCCTTCAATGTTACACCAAAGCCCTGCCCACACTCCAAAGGTCCTCCCTTGTGAAGAAGTCTAGGCAGAAGCCTCAAGAGCAGATGAGGGTCTTGACGGATAAACGCCGCCGAATGGCCACCGAGACACTTCCAGATTCCATCTTTATCTATGAGGAACGATCCTCCGCTCACCACATCAAATATGCAGCACAGGTTTATGAAAATGACTCTGACAACGACGGTGTGGAGAAGAAAGAGGAATTGACGGATCTTGGGGAGAGAGAGAAAATTGGGGAGAGAGAATGGAAAAGTAGTAATGGGTAA
Protein sequence

>MsG0480018180.01.T01

MDRGPDWSGLHQHGDDYLAHQRLAIVDSASGDQPLFSEDKSIIVLGSSELLMYDLSFATELCSLVSLQCYTKALPTLQRSSLVKKSRQKPQEQMRVLTDKRRRMATETLPDSIFIYEERSSAHHIKYAAQVYENDSDNDGVEKKEELTDLGEREKIGEREWKSSNG*