Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018222.01.T01 | KEH28564.1 | 83.333 | 132 | 16 | 3 | 1 | 127 | 1 | 131 | 2.62E-47 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018222.01.T01 | A0A072UFN5 | 83.333 | 132 | 16 | 3 | 1 | 127 | 1 | 131 | 1.25e-47 | 159 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280007272.01 | MsG0480018222.01 | 0.800562 | 1.360046e-48 | 6.352582e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018222.01.T01 | MTR_4g007570 | 83.333 | 132 | 16 | 3 | 1 | 127 | 1 | 131 | 3.17e-51 | 159 |
| MsG0480018222.01.T01 | MTR_4g007570 | 58.667 | 150 | 38 | 3 | 1 | 127 | 1 | 149 | 5.85e-45 | 143 |
| MsG0480018222.01.T01 | MTR_4g007580 | 86.719 | 128 | 16 | 1 | 1 | 127 | 1 | 128 | 1.14e-43 | 139 |
| MsG0480018222.01.T01 | MTR_4g007560 | 46.835 | 158 | 49 | 6 | 1 | 125 | 1 | 156 | 7.10e-18 | 75.1 |
| MsG0480018222.01.T01 | MTR_4g007590 | 38.621 | 145 | 66 | 4 | 1 | 126 | 1 | 141 | 3.32e-15 | 67.8 |
| MsG0480018222.01.T01 | MTR_4g007620 | 45.455 | 77 | 33 | 2 | 1 | 68 | 13 | 89 | 6.34e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACAAGCACATGAACTTAA+TGG | 0.248141 | 4:-1769554 | MsG0480018222.01.T01:CDS |
| ACTTCTTGCAACTTTCCTTC+TGG | 0.295526 | 4:-1769627 | MsG0480018222.01.T01:CDS |
| GCACATGAACTTAATGGAAA+TGG | 0.373587 | 4:-1769548 | MsG0480018222.01.T01:CDS |
| ATAAGAAAAGGAGGAGGAAT+AGG | 0.376818 | 4:-1769443 | MsG0480018222.01.T01:CDS |
| TAATGATGATGCAGAACTAT+TGG | 0.386268 | 4:+1769337 | None:intergenic |
| ATTATCGGTGCTGGAGTTAT+CGG | 0.395260 | 4:-1769389 | MsG0480018222.01.T01:CDS |
| TTTCCATCATCGCAACTTTG+TGG | 0.400584 | 4:+1769498 | None:intergenic |
| GGAGGAATAGGCAGAGGAAT+TGG | 0.409519 | 4:-1769431 | MsG0480018222.01.T01:CDS |
| GGAGCGGGTGCTGGAATTAT+CGG | 0.417075 | 4:-1769404 | MsG0480018222.01.T01:CDS |
| AGAGGAATTGGCAGAGGAGC+GGG | 0.433946 | 4:-1769419 | MsG0480018222.01.T01:CDS |
| ATTGGCAGAGGAGCGGGTGC+TGG | 0.442693 | 4:-1769413 | MsG0480018222.01.T01:CDS |
| GGAATGATGACTCCTGTCAT+AGG | 0.468039 | 4:+1769358 | None:intergenic |
| GAACCACAAAGTTGCGATGA+TGG | 0.489049 | 4:-1769501 | MsG0480018222.01.T01:CDS |
| CTATCTGTTACCATAAGAAA+AGG | 0.494904 | 4:-1769455 | MsG0480018222.01.T01:CDS |
| GGTGCTGGAATTATCGGTGC+TGG | 0.503051 | 4:-1769398 | MsG0480018222.01.T01:CDS |
| TCTGCATCATCATTATCTGC+AGG | 0.504126 | 4:-1769329 | MsG0480018222.01.T01:CDS |
| AATGTTGAGACACAAACATA+AGG | 0.523934 | 4:+1769305 | None:intergenic |
| AAAGGAGGAGGAATAGGCAG+AGG | 0.534351 | 4:-1769437 | MsG0480018222.01.T01:CDS |
| TCGGCGGGTCCGCCTATGAC+AGG | 0.543777 | 4:-1769370 | MsG0480018222.01.T01:CDS |
| ATAGGCAGAGGAATTGGCAG+AGG | 0.585765 | 4:-1769425 | MsG0480018222.01.T01:CDS |
| ATCGGTGCTGGAGTTATCGG+CGG | 0.587257 | 4:-1769386 | MsG0480018222.01.T01:CDS |
| CTGCATCATCATTATCTGCA+GGG | 0.603957 | 4:-1769328 | MsG0480018222.01.T01:CDS |
| CAGAGGAATTGGCAGAGGAG+CGG | 0.605437 | 4:-1769420 | MsG0480018222.01.T01:CDS |
| GAGCTCTTGAAATGACCAGA+AGG | 0.616315 | 4:+1769612 | None:intergenic |
| TCGGTGCTGGAGTTATCGGC+GGG | 0.625681 | 4:-1769385 | MsG0480018222.01.T01:CDS |
| TCTGTTACCATAAGAAAAGG+AGG | 0.631131 | 4:-1769452 | MsG0480018222.01.T01:CDS |
| ACTTAATGGAAATGGAAACA+TGG | 0.633060 | 4:-1769540 | MsG0480018222.01.T01:CDS |
| GTTACCATAAGAAAAGGAGG+AGG | 0.643572 | 4:-1769449 | MsG0480018222.01.T01:CDS |
| ATGATGACTCCTGTCATAGG+CGG | 0.670808 | 4:+1769361 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTTTATGTTTGAGCTTT+GGG | - | Chr4:1769632-1769651 | MsG0480018222.01.T01:CDS | 20.0% |
| !!! | TTTATTTTATGTTTGAGCTT+TGG | - | Chr4:1769631-1769650 | MsG0480018222.01.T01:CDS | 20.0% |
| AAACAAGCACATGAACTTAA+TGG | - | Chr4:1769361-1769380 | MsG0480018222.01.T01:CDS | 30.0% | |
| AATGTTGAGACACAAACATA+AGG | + | Chr4:1769613-1769632 | None:intergenic | 30.0% | |
| ACTTAATGGAAATGGAAACA+TGG | - | Chr4:1769375-1769394 | MsG0480018222.01.T01:CDS | 30.0% | |
| CTATCTGTTACCATAAGAAA+AGG | - | Chr4:1769460-1769479 | MsG0480018222.01.T01:CDS | 30.0% | |
| ! | TAATGATGATGCAGAACTAT+TGG | + | Chr4:1769581-1769600 | None:intergenic | 30.0% |
| ATAAGAAAAGGAGGAGGAAT+AGG | - | Chr4:1769472-1769491 | MsG0480018222.01.T01:CDS | 35.0% | |
| GCACATGAACTTAATGGAAA+TGG | - | Chr4:1769367-1769386 | MsG0480018222.01.T01:CDS | 35.0% | |
| TCTGTTACCATAAGAAAAGG+AGG | - | Chr4:1769463-1769482 | MsG0480018222.01.T01:CDS | 35.0% | |
| ! | TATTCCTCCTCCTTTTCTTA+TGG | + | Chr4:1769473-1769492 | None:intergenic | 35.0% |
| ACTTCTTGCAACTTTCCTTC+TGG | - | Chr4:1769288-1769307 | MsG0480018222.01.T01:CDS | 40.0% | |
| CTGCATCATCATTATCTGCA+GGG | - | Chr4:1769587-1769606 | MsG0480018222.01.T01:CDS | 40.0% | |
| GTTACCATAAGAAAAGGAGG+AGG | - | Chr4:1769466-1769485 | MsG0480018222.01.T01:CDS | 40.0% | |
| TCTGCATCATCATTATCTGC+AGG | - | Chr4:1769586-1769605 | MsG0480018222.01.T01:CDS | 40.0% | |
| TTTCCATCATCGCAACTTTG+TGG | + | Chr4:1769420-1769439 | None:intergenic | 40.0% | |
| !! | ATTATCGGTGCTGGAGTTAT+CGG | - | Chr4:1769526-1769545 | MsG0480018222.01.T01:CDS | 40.0% |
| ATGATGACTCCTGTCATAGG+CGG | + | Chr4:1769557-1769576 | None:intergenic | 45.0% | |
| GAACCACAAAGTTGCGATGA+TGG | - | Chr4:1769414-1769433 | MsG0480018222.01.T01:CDS | 45.0% | |
| GAGCTCTTGAAATGACCAGA+AGG | + | Chr4:1769306-1769325 | None:intergenic | 45.0% | |
| GGAATGATGACTCCTGTCAT+AGG | + | Chr4:1769560-1769579 | None:intergenic | 45.0% | |
| AAAGGAGGAGGAATAGGCAG+AGG | - | Chr4:1769478-1769497 | MsG0480018222.01.T01:CDS | 50.0% | |
| ATAGGCAGAGGAATTGGCAG+AGG | - | Chr4:1769490-1769509 | MsG0480018222.01.T01:CDS | 50.0% | |
| GGAGGAATAGGCAGAGGAAT+TGG | - | Chr4:1769484-1769503 | MsG0480018222.01.T01:CDS | 50.0% | |
| AGAGGAATTGGCAGAGGAGC+GGG | - | Chr4:1769496-1769515 | MsG0480018222.01.T01:CDS | 55.0% | |
| CAGAGGAATTGGCAGAGGAG+CGG | - | Chr4:1769495-1769514 | MsG0480018222.01.T01:CDS | 55.0% | |
| ! | GGTGCTGGAATTATCGGTGC+TGG | - | Chr4:1769517-1769536 | MsG0480018222.01.T01:CDS | 55.0% |
| !! | ATCGGTGCTGGAGTTATCGG+CGG | - | Chr4:1769529-1769548 | MsG0480018222.01.T01:CDS | 55.0% |
| !! | GGAGCGGGTGCTGGAATTAT+CGG | - | Chr4:1769511-1769530 | MsG0480018222.01.T01:CDS | 55.0% |
| !! | TCGGTGCTGGAGTTATCGGC+GGG | - | Chr4:1769530-1769549 | MsG0480018222.01.T01:CDS | 60.0% |
| ATTGGCAGAGGAGCGGGTGC+TGG | - | Chr4:1769502-1769521 | MsG0480018222.01.T01:CDS | 65.0% | |
| TCGGCGGGTCCGCCTATGAC+AGG | - | Chr4:1769545-1769564 | MsG0480018222.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 1769277 | 1769660 | 1769277 | ID=MsG0480018222.01;Name=MsG0480018222.01 |
| Chr4 | mRNA | 1769277 | 1769660 | 1769277 | ID=MsG0480018222.01.T01;Parent=MsG0480018222.01;Name=MsG0480018222.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|127 |
| Chr4 | exon | 1769277 | 1769660 | 1769277 | ID=MsG0480018222.01.T01:exon:1034;Parent=MsG0480018222.01.T01 |
| Chr4 | CDS | 1769277 | 1769660 | 1769277 | ID=MsG0480018222.01.T01:cds;Parent=MsG0480018222.01.T01 |
| Gene Sequence |
| Protein sequence |
