AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018227.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018227.01.T01 MTR_4g007710 60.965 228 50 4 45 233 102 329 4.06e-77 246
MsG0480018227.01.T01 MTR_4g007710 88.298 94 5 2 250 342 437 525 2.32e-43 157
MsG0480018227.01.T01 MTR_4g130310 37.654 162 62 8 50 172 100 261 4.15e-15 76.6
MsG0480018227.01.T01 MTR_4g130310 37.654 162 62 8 50 172 100 261 4.26e-15 76.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018227.01.T01 AT2G24100 28.534 382 166 15 46 336 100 465 1.08e-21 95.9
MsG0480018227.01.T01 AT4G30780 44.048 84 42 2 130 209 250 332 3.05e-11 65.1

Find 72 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAGAGTTGAAATCGAATT+TGG 0.132685 4:+1894015 None:intergenic
CAAGAGTAAAATGTTTGATT+TGG 0.243000 4:-1890648 MsG0480018227.01.T01:CDS
CAGAAGTGAACAAAATCTTA+TGG 0.288359 4:-1891960 MsG0480018227.01.T01:CDS
TTCTTCACAATTATCAACTA+TGG 0.296994 4:-1894136 MsG0480018227.01.T01:exon
CAATGCCCTCAAGGTCTGTT+AGG 0.323271 4:-1892199 MsG0480018227.01.T01:CDS
AAGAGTAAAATGTTTGATTT+GGG 0.335503 4:-1890647 MsG0480018227.01.T01:CDS
GGACTGCATGATGTGGAATC+AGG 0.352036 4:-1892007 MsG0480018227.01.T01:CDS
AGCTAGGTGAGGTAATTTCT+TGG 0.354560 4:+1891915 None:intergenic
AGAAGAAGTGAAGGGAAAGC+TGG 0.361169 4:-1892037 MsG0480018227.01.T01:CDS
CATTGAATTGAGTGATGGTT+TGG 0.363177 4:-1892062 MsG0480018227.01.T01:CDS
TGCCACAAGGTATGCTTCCT+TGG 0.393612 4:+1892545 None:intergenic
TTTCTGAAGAAGGCAGTATA+AGG 0.405521 4:+1890768 None:intergenic
CAATCAACAACAGACTTTAC+TGG 0.408931 4:-1892524 MsG0480018227.01.T01:CDS
GGAGAATCCAACACAAATTC+CGG 0.411628 4:+1892118 None:intergenic
GCAGTTCCAGTCTCAAAGTA+TGG 0.416139 4:+1892097 None:intergenic
CAAGATGATGATGATGCTTC+TGG 0.418720 4:-1890617 MsG0480018227.01.T01:CDS
TCAGAGTTGAAATCGAATTT+GGG 0.419622 4:+1894016 None:intergenic
CTATCAGAATCCTCGGACAT+AGG 0.423015 4:+1890497 None:intergenic
CCAACACAAATTCCGGTTGT+TGG 0.433853 4:+1892125 None:intergenic
GGTAGCCAGCAGCAAGGAAA+TGG 0.434094 4:-1890596 MsG0480018227.01.T01:CDS
AAGGTATGCTTCCTTGGTTG+TGG 0.434236 4:+1892551 None:intergenic
AGAAGTGAACAAAATCTTAT+GGG 0.452591 4:-1891959 MsG0480018227.01.T01:CDS
CAACTTTCTGAGCCAACAAC+CGG 0.458040 4:-1892137 MsG0480018227.01.T01:CDS
GATTCTCCATACTTTGAGAC+TGG 0.468589 4:-1892103 MsG0480018227.01.T01:CDS
TTATTTATACCTATGTATGC+TGG 0.472570 4:+1892493 None:intergenic
GAAGAAGTGAAGGGAAAGCT+GGG 0.473769 4:-1892036 MsG0480018227.01.T01:CDS
TGGTGTGTATTTGTTGTAGC+TGG 0.475721 4:-1892606 MsG0480018227.01.T01:CDS
ACAGACCATTTCCTTGCTGC+TGG 0.483280 4:+1890591 None:intergenic
CCAATCTTCAAAACAGTAGC+AGG 0.487934 4:+1893417 None:intergenic
TTTCAACTCTGATTCTCCTA+AGG 0.489774 4:-1894005 MsG0480018227.01.T01:intron
CCAACAACCGGAATTTGTGT+TGG 0.491090 4:-1892125 MsG0480018227.01.T01:CDS
GCCACTGTGCAATGCCCTCA+AGG 0.515589 4:-1892208 MsG0480018227.01.T01:CDS
ATTTGCTTCTGAATCTTCCA+AGG 0.519721 4:-1890547 MsG0480018227.01.T01:CDS
CACTCAATTCAATGTGGTCA+TGG 0.523533 4:+1892071 None:intergenic
TGTGGCAGAGAAGCAATCCT+TGG 0.523882 4:+1890530 None:intergenic
GAAATTACCTCACCTAGCTC+AGG 0.524612 4:-1891911 MsG0480018227.01.T01:intron
CATGATGTGGAATCAGGGTC+TGG 0.530089 4:-1892001 MsG0480018227.01.T01:CDS
GGAGAAGGTCTTTCTGAAGA+AGG 0.533229 4:+1890758 None:intergenic
TGCTGCTGATAGCAAGCTCA+AGG 0.539666 4:-1893451 MsG0480018227.01.T01:CDS
TTGGCTCAGAAAGTTGAGAC+GGG 0.539829 4:+1892144 None:intergenic
CTGTCTAGGAAAGAATCAGC+TGG 0.542082 4:-1890572 MsG0480018227.01.T01:CDS
GCCTCCCTAACAGACCTTGA+GGG 0.542641 4:+1892194 None:intergenic
AATGCCCTCAAGGTCTGTTA+GGG 0.546969 4:-1892198 MsG0480018227.01.T01:CDS
TGTATTTGTTGTAGCTGGCA+AGG 0.547557 4:-1892601 MsG0480018227.01.T01:CDS
GCTGGACATCGTAGAAAAGC+AGG 0.559213 4:-1894236 None:intergenic
GCCCTCAAGGTCTGTTAGGG+AGG 0.562789 4:-1892195 MsG0480018227.01.T01:CDS
GGTTTGGATAGAAGAAGTGA+AGG 0.566531 4:-1892046 MsG0480018227.01.T01:CDS
AACATGACTATCAGAATCCT+CGG 0.577952 4:+1890490 None:intergenic
TGCCTCCCTAACAGACCTTG+AGG 0.584503 4:+1892193 None:intergenic
GTGGACAAGCCAGCATACAT+AGG 0.584639 4:-1892502 MsG0480018227.01.T01:CDS
AGCAAGGAAATGGTCTGTCT+AGG 0.585074 4:-1890586 MsG0480018227.01.T01:CDS
CCATAGGAATTACCTGAGCT+AGG 0.591916 4:+1891899 None:intergenic
GACTGCATGATGTGGAATCA+GGG 0.597635 4:-1892006 MsG0480018227.01.T01:CDS
GACATAGGAAACAGAAAATG+TGG 0.600386 4:+1890512 None:intergenic
GTTGGCTCAGAAAGTTGAGA+CGG 0.604139 4:+1892143 None:intergenic
GAAGATAGAAAAGTTGATGA+GGG 0.606665 4:-1890686 MsG0480018227.01.T01:CDS
TTTGAAGATTGGTACTTGGG+AGG 0.609526 4:-1893406 MsG0480018227.01.T01:intron
GACCACATTGAATTGAGTGA+TGG 0.611625 4:-1892067 MsG0480018227.01.T01:CDS
TGAAGATAGAAAAGTTGATG+AGG 0.612436 4:-1890687 MsG0480018227.01.T01:CDS
GTCTGTTGTTGATTGCCACA+AGG 0.622596 4:+1892532 None:intergenic
TCAACAACAGACTTTACTGG+TGG 0.640598 4:-1892521 MsG0480018227.01.T01:CDS
GTTTGGATAGAAGAAGTGAA+GGG 0.651890 4:-1892045 MsG0480018227.01.T01:CDS
ATTTGTTGTAGCTGGCAAGG+AGG 0.658694 4:-1892598 MsG0480018227.01.T01:CDS
GGGAGATCAATCCACAACCA+AGG 0.673080 4:-1892562 MsG0480018227.01.T01:CDS
GCTTCTGGTAGCCAGCAGCA+AGG 0.673116 4:-1890602 MsG0480018227.01.T01:CDS
ATGAACAGAATGTCATGACA+AGG 0.678824 4:-1890798 MsG0480018227.01.T01:intron
GGAATTACCTGAGCTAGGTG+AGG 0.680636 4:+1891904 None:intergenic
TGGCTCAGAAAGTTGAGACG+GGG 0.681167 4:+1892145 None:intergenic
AACCATCACTCAATTCAATG+TGG 0.718712 4:+1892065 None:intergenic
ACCTTGAGGGCATTGCACAG+TGG 0.733071 4:+1892207 None:intergenic
TGAACAGAATGTCATGACAA+GGG 0.758366 4:-1890797 MsG0480018227.01.T01:intron
AACCAAGGAAGCATACCTTG+TGG 0.758682 4:-1892547 MsG0480018227.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTGTTAATTTCATAT+TGG - Chr4:1890853-1890872 MsG0480018227.01.T01:intron 10.0%
!!! TTTTAATTTCAAATTTATCA+TGG - Chr4:1892953-1892972 MsG0480018227.01.T01:intron 10.0%
!!! TTTTATACATTGTTTTAATT+TGG - Chr4:1892844-1892863 MsG0480018227.01.T01:intron 10.0%
!! AAATAAGTTATTTATCAAAC+CGG + Chr4:1891397-1891416 None:intergenic 15.0%
!! AGAGGAATAATATTATAAAA+TGG + Chr4:1893452-1893471 None:intergenic 15.0%
!! TAATCATTAGCATTATATAA+TGG - Chr4:1893508-1893527 MsG0480018227.01.T01:intron 15.0%
!! TGTATAAAAAAAATATCCAT+AGG + Chr4:1892833-1892852 None:intergenic 15.0%
!!! ATATGGAATTATATCATTTT+CGG + Chr4:1893624-1893643 None:intergenic 15.0%
!!! TTTTATTGCTTAATAGATTT+TGG - Chr4:1891820-1891839 MsG0480018227.01.T01:intron 15.0%
!! AAATAGCATATTGCAAAATA+TGG + Chr4:1891984-1892003 None:intergenic 20.0%
!! AATAAGTTATTTATCAAACC+GGG + Chr4:1891396-1891415 None:intergenic 20.0%
!! AATTTAATCATTTGATACTC+TGG + Chr4:1893040-1893059 None:intergenic 20.0%
!! TAATTTATTGTGGATATCTA+CGG - Chr4:1893334-1893353 MsG0480018227.01.T01:intron 20.0%
!! TATCTCAAGAAATACAAAAA+AGG + Chr4:1892897-1892916 None:intergenic 20.0%
!!! AAGAGTAAAATGTTTGATTT+GGG - Chr4:1894066-1894085 MsG0480018227.01.T01:CDS 20.0%
!!! AATTGTGTTTTTTGTTTGTT+TGG - Chr4:1891057-1891076 MsG0480018227.01.T01:intron 20.0%
!!! AGAGTATCAAATGATTAAAT+TGG - Chr4:1893039-1893058 MsG0480018227.01.T01:intron 20.0%
!!! ATCTTTTAAATTTTCCTGAA+TGG - Chr4:1892989-1893008 MsG0480018227.01.T01:intron 20.0%
!!! ATTCTGTATTTTGTTGTTAT+AGG - Chr4:1892481-1892500 MsG0480018227.01.T01:CDS 20.0%
!!! ATTGTGTTTTTTTGTTTGTT+TGG - Chr4:1890939-1890958 MsG0480018227.01.T01:intron 20.0%
!!! GTTTAATTTTCAGTTTGTTA+TGG - Chr4:1890878-1890897 MsG0480018227.01.T01:intron 20.0%
!!! TGTTGATTATGTTTTTGTTA+TGG - Chr4:1893380-1893399 MsG0480018227.01.T01:intron 20.0%
!!! TTTAGTTGTTTTCAATTCAT+TGG - Chr4:1892421-1892440 MsG0480018227.01.T01:intron 20.0%
!!! TTTTGTTAATTTCATATTGG+TGG - Chr4:1890856-1890875 MsG0480018227.01.T01:intron 20.0%
!!! TTTTGTTTGTAGAAATATGA+AGG - Chr4:1891370-1891389 MsG0480018227.01.T01:intron 20.0%
! AACAACTGATTGTATGAAAT+CGG + Chr4:1890994-1891013 None:intergenic 25.0%
! AATGCATTAAGATCTATTGA+GGG + Chr4:1893278-1893297 None:intergenic 25.0%
! AATTTATTGTGGATATCTAC+GGG - Chr4:1893335-1893354 MsG0480018227.01.T01:intron 25.0%
! AGAAGTGAACAAAATCTTAT+GGG - Chr4:1892754-1892773 MsG0480018227.01.T01:intron 25.0%
! ATACAAAAAAGGCTATAAAC+AGG + Chr4:1892886-1892905 None:intergenic 25.0%
! ATCACTTCAAAAAGTCAAAT+TGG - Chr4:1890630-1890649 MsG0480018227.01.T01:CDS 25.0%
! ATTAAATTGGCAGTATTGTT+TGG - Chr4:1893052-1893071 MsG0480018227.01.T01:intron 25.0%
! CAAGAGTAAAATGTTTGATT+TGG - Chr4:1894065-1894084 MsG0480018227.01.T01:CDS 25.0%
! CTGCAGATGATAATAAAATA+TGG + Chr4:1893641-1893660 None:intergenic 25.0%
! GTGTTAGATATATGCTATAA+TGG - Chr4:1891927-1891946 MsG0480018227.01.T01:CDS 25.0%
! TTATTTATACCTATGTATGC+TGG + Chr4:1892223-1892242 None:intergenic 25.0%
! TTCTTCACAATTATCAACTA+TGG - Chr4:1890577-1890596 MsG0480018227.01.T01:CDS 25.0%
! TTTCATACAATCAGTTGTTA+TGG - Chr4:1890995-1891014 MsG0480018227.01.T01:intron 25.0%
!! CATTACATGTGTCTAAAATA+TGG - Chr4:1893552-1893571 MsG0480018227.01.T01:intron 25.0%
!! GTTGTCTTCATAAGTTATTT+TGG - Chr4:1891510-1891529 MsG0480018227.01.T01:intron 25.0%
!! TTTAAATTTTCCTGAATGGT+CGG - Chr4:1892993-1893012 MsG0480018227.01.T01:intron 25.0%
!!! ATTGTTTTAATTTGGTCTGA+TGG - Chr4:1892852-1892871 MsG0480018227.01.T01:intron 25.0%
AAAGCAGGAGAAAAACAATT+TGG - Chr4:1890492-1890511 MsG0480018227.01.T01:CDS 30.0%
AATATTATTCCTCTGTTCTG+AGG - Chr4:1893458-1893477 MsG0480018227.01.T01:CDS 30.0%
AATCACATCATTAAGACATC+AGG + Chr4:1892464-1892483 None:intergenic 30.0%
ACCCAGAATGAAATTAATGA+TGG + Chr4:1890773-1890792 None:intergenic 30.0%
ATCAGAGTTGAAATCGAATT+TGG + Chr4:1890701-1890720 None:intergenic 30.0%
ATCCAAAGGATTGTATGAAT+CGG + Chr4:1891112-1891131 None:intergenic 30.0%
ATGCATTAAGATCTATTGAG+GGG + Chr4:1893277-1893296 None:intergenic 30.0%
ATTGACAACATATGCTAAGT+TGG - Chr4:1892087-1892106 MsG0480018227.01.T01:CDS 30.0%
CACCATATCAAAACTTATGA+TGG - Chr4:1891603-1891622 MsG0480018227.01.T01:intron 30.0%
CAGAAGTGAACAAAATCTTA+TGG - Chr4:1892753-1892772 MsG0480018227.01.T01:intron 30.0%
CCAGAATGAAATTAATGATG+GGG + Chr4:1890771-1890790 None:intergenic 30.0%
CCCAGAATGAAATTAATGAT+GGG + Chr4:1890772-1890791 None:intergenic 30.0%
CCCATCATTAATTTCATTCT+GGG - Chr4:1890769-1890788 MsG0480018227.01.T01:CDS 30.0%
GAAGAAAAACAGGTTCAAAA+TGG - Chr4:1893982-1894001 MsG0480018227.01.T01:intron 30.0%
GAATGCATTAAGATCTATTG+AGG + Chr4:1893279-1893298 None:intergenic 30.0%
TAATGATTAAAGACAGTAGC+AGG + Chr4:1893497-1893516 None:intergenic 30.0%
TAGATTGTACCTAGACTAAA+TGG + Chr4:1893162-1893181 None:intergenic 30.0%
TCAGAGTTGAAATCGAATTT+GGG + Chr4:1890700-1890719 None:intergenic 30.0%
TCGGTGATAATAAAATCACA+AGG + Chr4:1893605-1893624 None:intergenic 30.0%
TGAAAGAAAAAGAGAACCTT+AGG + Chr4:1890727-1890746 None:intergenic 30.0%
TTGATCAGTTAATTGTGTCA+TGG - Chr4:1891784-1891803 MsG0480018227.01.T01:intron 30.0%
TTGTCAAATAGCTTATGTAG+CGG - Chr4:1892014-1892033 MsG0480018227.01.T01:CDS 30.0%
TTTCTGATTAATCTGCAATC+CGG - Chr4:1893229-1893248 MsG0480018227.01.T01:intron 30.0%
! ATGAAATTAATGATGGGGTT+TGG + Chr4:1890766-1890785 None:intergenic 30.0%
! CATAAGATTTTGTTCACTTC+TGG + Chr4:1892755-1892774 None:intergenic 30.0%
! GTGATGATGTGATGTTATTT+AGG - Chr4:1891636-1891655 MsG0480018227.01.T01:intron 30.0%
!! CACCATCATAAGTTTTGATA+TGG + Chr4:1891608-1891627 None:intergenic 30.0%
!! GAAGATAGAAAAGTTGATGA+GGG - Chr4:1894027-1894046 MsG0480018227.01.T01:CDS 30.0%
!! TCAATTCATTGGTAATGAAG+TGG - Chr4:1892432-1892451 MsG0480018227.01.T01:intron 30.0%
!! TGAAGATAGAAAAGTTGATG+AGG - Chr4:1894026-1894045 MsG0480018227.01.T01:CDS 30.0%
!! TTTTGAACCTGTTTTTCTTC+AGG + Chr4:1893982-1894001 None:intergenic 30.0%
!!! TGTTTTGAAGATTGGTACTT+GGG - Chr4:1891304-1891323 MsG0480018227.01.T01:intron 30.0%
AAATCTCGACCATTTAGTCT+AGG - Chr4:1893150-1893169 MsG0480018227.01.T01:intron 35.0%
AACATGACTATCAGAATCCT+CGG + Chr4:1894226-1894245 None:intergenic 35.0%
AACCATCACTCAATTCAATG+TGG + Chr4:1892651-1892670 None:intergenic 35.0%
AATCAAAAACCTCAGAACAG+AGG + Chr4:1893470-1893489 None:intergenic 35.0%
ATGAACAGAATGTCATGACA+AGG - Chr4:1893915-1893934 MsG0480018227.01.T01:intron 35.0%
ATTGATCTCCCTGAAAAACA+GGG + Chr4:1892142-1892161 None:intergenic 35.0%
ATTTGCTTCTGAATCTTCCA+AGG - Chr4:1894166-1894185 MsG0480018227.01.T01:five_prime_UTR 35.0%
CAATCAACAACAGACTTTAC+TGG - Chr4:1892189-1892208 MsG0480018227.01.T01:CDS 35.0%
CATTGAATTGAGTGATGGTT+TGG - Chr4:1892651-1892670 MsG0480018227.01.T01:intron 35.0%
CCCCATCATTAATTTCATTC+TGG - Chr4:1890768-1890787 MsG0480018227.01.T01:CDS 35.0%
CTGAGAAACTTATCAGATTC+AGG + Chr4:1893704-1893723 None:intergenic 35.0%
GACATAGGAAACAGAAAATG+TGG + Chr4:1894204-1894223 None:intergenic 35.0%
GCAGTATAAGTCTAGATATG+AGG - Chr4:1891663-1891682 MsG0480018227.01.T01:intron 35.0%
GTTTGGATAGAAGAAGTGAA+GGG - Chr4:1892668-1892687 MsG0480018227.01.T01:intron 35.0%
TAAACAAATCTGTGCACTCT+TGG + Chr4:1892400-1892419 None:intergenic 35.0%
TAGACTAAATGGTCGAGATT+TGG + Chr4:1893151-1893170 None:intergenic 35.0%
TAGGTACAATCTACATCCAA+TGG - Chr4:1893169-1893188 MsG0480018227.01.T01:intron 35.0%
TCCAAGAAGAAAGATCAGAA+GGG - Chr4:1891216-1891235 MsG0480018227.01.T01:intron 35.0%
TCTAGATATGAGGAGATTTG+TGG - Chr4:1891673-1891692 MsG0480018227.01.T01:intron 35.0%
TGAACAGAATGTCATGACAA+GGG - Chr4:1893916-1893935 MsG0480018227.01.T01:intron 35.0%
TTCCAAGAAGAAAGATCAGA+AGG - Chr4:1891215-1891234 MsG0480018227.01.T01:intron 35.0%
TTCTGATTAATCTGCAATCC+GGG - Chr4:1893230-1893249 MsG0480018227.01.T01:intron 35.0%
TTTCAACTCTGATTCTCCTA+AGG - Chr4:1890708-1890727 MsG0480018227.01.T01:CDS 35.0%
TTTGGATCAGTTATGTGTCA+TGG - Chr4:1891838-1891857 MsG0480018227.01.T01:intron 35.0%
TTTGTAGAAATATGAAGGCC+CGG - Chr4:1891375-1891394 MsG0480018227.01.T01:intron 35.0%
! AAGATTTTGTTCACTTCTGG+AGG + Chr4:1892752-1892771 None:intergenic 35.0%
! TTAGTGAAGCTGGAATTTTC+AGG + Chr4:1893824-1893843 None:intergenic 35.0%
! TTTCTGAAGAAGGCAGTATA+AGG + Chr4:1893948-1893967 None:intergenic 35.0%
!! GATGATTTGGTTAACCACAT+CGG - Chr4:1893773-1893792 MsG0480018227.01.T01:intron 35.0%
!!! CTGTTTTGAAGATTGGTACT+TGG - Chr4:1891303-1891322 MsG0480018227.01.T01:intron 35.0%
AAATCATCCATCGACATCGA+AGG + Chr4:1893761-1893780 None:intergenic 40.0%
AAATCTGTGCACTCTTGGAT+TGG + Chr4:1892395-1892414 None:intergenic 40.0%
AAGAAGAGTCCTTCACTGTT+GGG - Chr4:1891158-1891177 MsG0480018227.01.T01:intron 40.0%
AATCATCCATCGACATCGAA+GGG + Chr4:1893760-1893779 None:intergenic 40.0%
ACCCTTCTGATCTTTCTTCT+TGG + Chr4:1891220-1891239 None:intergenic 40.0%
AGCTAGGTGAGGTAATTTCT+TGG + Chr4:1892801-1892820 None:intergenic 40.0%
ATAATCAACACTAGTGCAGC+TGG + Chr4:1893370-1893389 None:intergenic 40.0%
ATTTGCATGCACAGGTTCAA+CGG - Chr4:1893197-1893216 MsG0480018227.01.T01:intron 40.0%
CACTCAATTCAATGTGGTCA+TGG + Chr4:1892645-1892664 None:intergenic 40.0%
CCAATCTTCAAAACAGTAGC+AGG + Chr4:1891299-1891318 None:intergenic 40.0%
CCTGAAACCTTTGATTGCAT+GGG + Chr4:1893672-1893691 None:intergenic 40.0%
CTGAAACCTTTGATTGCATG+GGG + Chr4:1893671-1893690 None:intergenic 40.0%
GAATCAAATCCCAACAGTGA+AGG + Chr4:1891170-1891189 None:intergenic 40.0%
GAATGGCGGTAAAATCAGAA+CGG - Chr4:1892283-1892302 MsG0480018227.01.T01:intron 40.0%
GACCACATTGAATTGAGTGA+TGG - Chr4:1892646-1892665 MsG0480018227.01.T01:intron 40.0%
GATTAACCCCATGCAATCAA+AGG - Chr4:1893662-1893681 MsG0480018227.01.T01:intron 40.0%
GATTGATCTCCCTGAAAAAC+AGG + Chr4:1892143-1892162 None:intergenic 40.0%
GGAGAATCCAACACAAATTC+CGG + Chr4:1892598-1892617 None:intergenic 40.0%
GGCAGTATTGTTTGGTTGTT+TGG - Chr4:1893060-1893079 MsG0480018227.01.T01:intron 40.0%
GGTTTGGATAGAAGAAGTGA+AGG - Chr4:1892667-1892686 MsG0480018227.01.T01:intron 40.0%
GTCCGATTCATACAATCCTT+TGG - Chr4:1891107-1891126 MsG0480018227.01.T01:intron 40.0%
TCAACAACAGACTTTACTGG+TGG - Chr4:1892192-1892211 MsG0480018227.01.T01:CDS 40.0%
TCCTGAAACCTTTGATTGCA+TGG + Chr4:1893673-1893692 None:intergenic 40.0%
TCTCAAGTGCATGATTGGAA+TGG - Chr4:1892266-1892285 MsG0480018227.01.T01:intron 40.0%
TGCATGCAAATCTCAACCAT+TGG + Chr4:1893188-1893207 None:intergenic 40.0%
TGGGATCAGTTATGTGTCAT+GGG - Chr4:1891730-1891749 MsG0480018227.01.T01:intron 40.0%
TGTCATCTCAAGTGCATGAT+TGG - Chr4:1892261-1892280 MsG0480018227.01.T01:intron 40.0%
TGTGATTCAGGTGATGATTC+TGG - Chr4:1890967-1890986 MsG0480018227.01.T01:intron 40.0%
TTCGATGTCGATGGATGATT+TGG - Chr4:1893760-1893779 MsG0480018227.01.T01:intron 40.0%
TTCTCCACCTGAAGAAAAAC+AGG - Chr4:1893972-1893991 MsG0480018227.01.T01:intron 40.0%
TTCTGTTCATCATCAGAGAC+AGG + Chr4:1893905-1893924 None:intergenic 40.0%
TTGATCTCCCTGAAAAACAG+GGG + Chr4:1892141-1892160 None:intergenic 40.0%
TTGGGATCAGTTATGTGTCA+TGG - Chr4:1891729-1891748 MsG0480018227.01.T01:intron 40.0%
TTTAGATCAACCCTCAATGC+CGG - Chr4:1893097-1893116 MsG0480018227.01.T01:intron 40.0%
TTTGAAGATTGGTACTTGGG+AGG - Chr4:1891307-1891326 MsG0480018227.01.T01:intron 40.0%
! CAAGATGATGATGATGCTTC+TGG - Chr4:1894096-1894115 MsG0480018227.01.T01:CDS 40.0%
! CGAAGTGGCTACTAGATTTT+GGG - Chr4:1891711-1891730 MsG0480018227.01.T01:intron 40.0%
! TGTTTGGAACAGTGTGATTC+AGG - Chr4:1890955-1890974 MsG0480018227.01.T01:intron 40.0%
! TGTTTTTCTTCAGGTGGAGA+AGG + Chr4:1893973-1893992 None:intergenic 40.0%
! TTGAGTCAGATTAAGTTGCC+TGG - Chr4:1893728-1893747 MsG0480018227.01.T01:intron 40.0%
! TTTTCTGTTTCCTATGTCCG+AGG - Chr4:1894206-1894225 MsG0480018227.01.T01:five_prime_UTR 40.0%
!! CGAAATGTTACTTTGCGAAG+TGG - Chr4:1891696-1891715 MsG0480018227.01.T01:intron 40.0%
!! GATTCTCCATACTTTGAGAC+TGG - Chr4:1892610-1892629 MsG0480018227.01.T01:CDS 40.0%
!! GATTTTTGGACTGCATGATG+TGG - Chr4:1892699-1892718 MsG0480018227.01.T01:intron 40.0%
!! TGAACCTGTTTTTCTTCAGG+TGG + Chr4:1893979-1893998 None:intergenic 40.0%
!! TGGTGTGTATTTGTTGTAGC+TGG - Chr4:1892107-1892126 MsG0480018227.01.T01:CDS 40.0%
!! TGTATTTGTTGTAGCTGGCA+AGG - Chr4:1892112-1892131 MsG0480018227.01.T01:CDS 40.0%
!!! CCTGCTACTGTTTTGAAGAT+TGG - Chr4:1891296-1891315 MsG0480018227.01.T01:intron 40.0%
AAGGTATGCTTCCTTGGTTG+TGG + Chr4:1892165-1892184 None:intergenic 45.0%
AATGCCCTCAAGGTCTGTTA+GGG - Chr4:1892515-1892534 MsG0480018227.01.T01:CDS 45.0%
ACACTGCATTTCGGAACAGA+TGG - Chr4:1893796-1893815 MsG0480018227.01.T01:intron 45.0%
AGAAGAAGTGAAGGGAAAGC+TGG - Chr4:1892676-1892695 MsG0480018227.01.T01:intron 45.0%
AGCAAGGAAATGGTCTGTCT+AGG - Chr4:1894127-1894146 MsG0480018227.01.T01:exon 45.0%
AGCTCTGTCATTAGTGAAGC+TGG + Chr4:1893834-1893853 None:intergenic 45.0%
ATCAAAGGTTTCAGGAGCAG+AGG - Chr4:1893677-1893696 MsG0480018227.01.T01:intron 45.0%
ATTAATCTGCAATCCGGGTG+AGG - Chr4:1893235-1893254 MsG0480018227.01.T01:intron 45.0%
ATTTGGCTGCACCAGCTATA+TGG + Chr4:1893134-1893153 None:intergenic 45.0%
CAACTTTCTGAGCCAACAAC+CGG - Chr4:1892576-1892595 MsG0480018227.01.T01:CDS 45.0%
CAAGTGCATGATTGGAATGG+CGG - Chr4:1892269-1892288 MsG0480018227.01.T01:intron 45.0%
CACTAATGACAGAGCTGTGT+TGG - Chr4:1893838-1893857 MsG0480018227.01.T01:intron 45.0%
CACTGCATTTCGGAACAGAT+GGG - Chr4:1893797-1893816 MsG0480018227.01.T01:intron 45.0%
CCAACAACCGGAATTTGTGT+TGG - Chr4:1892588-1892607 MsG0480018227.01.T01:CDS 45.0%
CCAACACAAATTCCGGTTGT+TGG + Chr4:1892591-1892610 None:intergenic 45.0%
CCATAGGAATTACCTGAGCT+AGG + Chr4:1892817-1892836 None:intergenic 45.0%
CCCATGCAATCAAAGGTTTC+AGG - Chr4:1893669-1893688 MsG0480018227.01.T01:intron 45.0%
CCTAGCTCAGGTAATTCCTA+TGG - Chr4:1892814-1892833 MsG0480018227.01.T01:intron 45.0%
CTATCAGAATCCTCGGACAT+AGG + Chr4:1894219-1894238 None:intergenic 45.0%
CTGTCTAGGAAAGAATCAGC+TGG - Chr4:1894141-1894160 MsG0480018227.01.T01:five_prime_UTR 45.0%
GAAATTACCTCACCTAGCTC+AGG - Chr4:1892802-1892821 MsG0480018227.01.T01:intron 45.0%
GAAGAAGAGTCCTTCACTGT+TGG - Chr4:1891157-1891176 MsG0480018227.01.T01:intron 45.0%
GAAGAAGTGAAGGGAAAGCT+GGG - Chr4:1892677-1892696 MsG0480018227.01.T01:intron 45.0%
GACTGCATGATGTGGAATCA+GGG - Chr4:1892707-1892726 MsG0480018227.01.T01:intron 45.0%
GCAGTTCCAGTCTCAAAGTA+TGG + Chr4:1892619-1892638 None:intergenic 45.0%
TAATGACAGAGCTGTGTTGG+AGG - Chr4:1893841-1893860 MsG0480018227.01.T01:intron 45.0%
TACAGTTTCACCGACCATTC+AGG + Chr4:1893006-1893025 None:intergenic 45.0%
TGGTTGAGATTTGCATGCAC+AGG - Chr4:1893189-1893208 MsG0480018227.01.T01:intron 45.0%
TGTTCATCATCAGAGACAGG+TGG + Chr4:1893902-1893921 None:intergenic 45.0%
TTAATCTGCAATCCGGGTGA+GGG - Chr4:1893236-1893255 MsG0480018227.01.T01:intron 45.0%
TTGGATGAACCAAGTCCACT+TGG - Chr4:1891126-1891145 MsG0480018227.01.T01:intron 45.0%
TTGGCTCAGAAAGTTGAGAC+GGG + Chr4:1892572-1892591 None:intergenic 45.0%
! AACCAAGGAAGCATACCTTG+TGG - Chr4:1892166-1892185 MsG0480018227.01.T01:CDS 45.0%
! AGTGGACTTGGTTCATCCAA+AGG + Chr4:1891126-1891145 None:intergenic 45.0%
! GAAGGGAAAGCTGGGATTTT+TGG - Chr4:1892685-1892704 MsG0480018227.01.T01:intron 45.0%
! GCGAAGTGGCTACTAGATTT+TGG - Chr4:1891710-1891729 MsG0480018227.01.T01:intron 45.0%
! GGAGAAGGTCTTTCTGAAGA+AGG + Chr4:1893958-1893977 None:intergenic 45.0%
! GTCTGTTGTTGATTGCCACA+AGG + Chr4:1892184-1892203 None:intergenic 45.0%
! GTTGGCTCAGAAAGTTGAGA+CGG + Chr4:1892573-1892592 None:intergenic 45.0%
!! ATTTGTTGTAGCTGGCAAGG+AGG - Chr4:1892115-1892134 MsG0480018227.01.T01:CDS 45.0%
!!! ATAAATTTTTTAATTTATTG+TGG - Chr4:1893324-1893343 MsG0480018227.01.T01:intron 5.0%
ACAGACCATTTCCTTGCTGC+TGG + Chr4:1894125-1894144 None:intergenic 50.0%
CAATGCCCTCAAGGTCTGTT+AGG - Chr4:1892514-1892533 MsG0480018227.01.T01:CDS 50.0%
CACAGGTTCAACGGTTGTAG+AGG - Chr4:1893206-1893225 MsG0480018227.01.T01:intron 50.0%
CATGATGTGGAATCAGGGTC+TGG - Chr4:1892712-1892731 MsG0480018227.01.T01:intron 50.0%
CCACATCGGACACTGCATTT+CGG - Chr4:1893787-1893806 MsG0480018227.01.T01:intron 50.0%
CTTCTTCAGACGCAACCAAG+TGG + Chr4:1891144-1891163 None:intergenic 50.0%
GGAATTACCTGAGCTAGGTG+AGG + Chr4:1892812-1892831 None:intergenic 50.0%
GGACTGCATGATGTGGAATC+AGG - Chr4:1892706-1892725 MsG0480018227.01.T01:intron 50.0%
GGGAGATCAATCCACAACCA+AGG - Chr4:1892151-1892170 MsG0480018227.01.T01:CDS 50.0%
GTGGACAAGCCAGCATACAT+AGG - Chr4:1892211-1892230 MsG0480018227.01.T01:CDS 50.0%
TAAAACTTCCCTCCCTCACC+CGG + Chr4:1893251-1893270 None:intergenic 50.0%
TCTTCACCCTTCGATGTCGA+TGG - Chr4:1893751-1893770 MsG0480018227.01.T01:intron 50.0%
TGCCACAAGGTATGCTTCCT+TGG + Chr4:1892171-1892190 None:intergenic 50.0%
TGCTGCTGATAGCAAGCTCA+AGG - Chr4:1891262-1891281 MsG0480018227.01.T01:intron 50.0%
TGGCTCAGAAAGTTGAGACG+GGG + Chr4:1892571-1892590 None:intergenic 50.0%
TGTGGCAGAGAAGCAATCCT+TGG + Chr4:1894186-1894205 None:intergenic 50.0%
!! AAGGAGGCCCCTGTTTTTCA+GGG - Chr4:1892131-1892150 MsG0480018227.01.T01:CDS 50.0%
AACCCTCAATGCCGGATCCT+CGG - Chr4:1893105-1893124 MsG0480018227.01.T01:intron 55.0%
ATCCTCGGCAGCCATATAGC+TGG - Chr4:1893120-1893139 MsG0480018227.01.T01:intron 55.0%
CCGAAATGCAGTGTCCGATG+TGG + Chr4:1893790-1893809 None:intergenic 55.0%
CTATATGGCTGCCGAGGATC+CGG + Chr4:1893119-1893138 None:intergenic 55.0%
GACATCGAAGGGTGAAGACC+AGG + Chr4:1893749-1893768 None:intergenic 55.0%
GCCTCCCTAACAGACCTTGA+GGG + Chr4:1892522-1892541 None:intergenic 55.0%
GGTAGCCAGCAGCAAGGAAA+TGG - Chr4:1894117-1894136 MsG0480018227.01.T01:CDS 55.0%
TGCCTCCCTAACAGACCTTG+AGG + Chr4:1892523-1892542 None:intergenic 55.0%
! CAGACGCAACCAAGTGGACT+TGG + Chr4:1891138-1891157 None:intergenic 55.0%
!! ACCTTGAGGGCATTGCACAG+TGG + Chr4:1892509-1892528 None:intergenic 55.0%
!! CAAGGAGGCCCCTGTTTTTC+AGG - Chr4:1892130-1892149 MsG0480018227.01.T01:CDS 55.0%
ATCTGCAATCCGGGTGAGGG+AGG - Chr4:1893239-1893258 MsG0480018227.01.T01:intron 60.0%
CACCAGCTATATGGCTGCCG+AGG + Chr4:1893125-1893144 None:intergenic 60.0%
GCCACTGTGCAATGCCCTCA+AGG - Chr4:1892505-1892524 MsG0480018227.01.T01:CDS 60.0%
GCCCTCAAGGTCTGTTAGGG+AGG - Chr4:1892518-1892537 MsG0480018227.01.T01:CDS 60.0%
GCTTCTGGTAGCCAGCAGCA+AGG - Chr4:1894111-1894130 MsG0480018227.01.T01:CDS 60.0%
TCTGCAATCCGGGTGAGGGA+GGG - Chr4:1893240-1893259 MsG0480018227.01.T01:intron 60.0%
! TGCCGAGGATCCGGCATTGA+GGG + Chr4:1893110-1893129 None:intergenic 60.0%
CTGCCGAGGATCCGGCATTG+AGG + Chr4:1893111-1893130 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 1890484 1894251 1890484 ID=MsG0480018227.01;Name=MsG0480018227.01
Chr4 mRNA 1890484 1894251 1890484 ID=MsG0480018227.01.T01;Parent=MsG0480018227.01;Name=MsG0480018227.01.T01;_AED=0.34;_eAED=0.42;_QI=112|0|0.4|1|0.25|0.6|5|0|342
Chr4 exon 1890484 1890812 1890484 ID=MsG0480018227.01.T01:exon:1056;Parent=MsG0480018227.01.T01
Chr4 exon 1891912 1892232 1891912 ID=MsG0480018227.01.T01:exon:1055;Parent=MsG0480018227.01.T01
Chr4 exon 1892464 1892629 1892464 ID=MsG0480018227.01.T01:exon:1054;Parent=MsG0480018227.01.T01
Chr4 exon 1893407 1893485 1893407 ID=MsG0480018227.01.T01:exon:1053;Parent=MsG0480018227.01.T01
Chr4 exon 1894006 1894251 1894006 ID=MsG0480018227.01.T01:exon:1052;Parent=MsG0480018227.01.T01
Chr4 five_prime_UTR 1894140 1894251 1894140 ID=MsG0480018227.01.T01:five_prime_utr;Parent=MsG0480018227.01.T01
Chr4 CDS 1894006 1894139 1894006 ID=MsG0480018227.01.T01:cds;Parent=MsG0480018227.01.T01
Chr4 CDS 1893407 1893485 1893407 ID=MsG0480018227.01.T01:cds;Parent=MsG0480018227.01.T01
Chr4 CDS 1892464 1892629 1892464 ID=MsG0480018227.01.T01:cds;Parent=MsG0480018227.01.T01
Chr4 CDS 1891912 1892232 1891912 ID=MsG0480018227.01.T01:cds;Parent=MsG0480018227.01.T01
Chr4 CDS 1890484 1890812 1890484 ID=MsG0480018227.01.T01:cds;Parent=MsG0480018227.01.T01
Gene Sequence

>MsG0480018227.01.T01

ATGGTTCAACTCATGAGATCTGATTCTGATAATAATCACTTCAAAAAGTCAAATTGGAATCTCAACAAGATGATGATCATCAACTTCATTCTAACAAGCGTCCCAAATTCGATTTCAACTCTGATTCTCCTAAGCTTCGCTGCTGCTGCTGCTGATAGCAAGCTCAAGGCTTCAAATTTTCCTGCTACTGTTTTGAAGATTGGTACTTGGGAGTTGGTGTGTATTTGTTGTAGCTGGCAAGGAGGCCCCTGTTTTTCAGGGAGATCAATCCACAACCAAGGAAGCATACCTTGTGGCAATCAACAACAGACTTTACTGGTGGACAAGCCAGCATACATAGGTATAAATAAATATTTGTCGAAATTATCTGTCATCTCAAGCGCCACTGTGCAATGCCCTCAAGGTCTGTTAGGGAGGCATTTTGAGAAGCTCATACAGTGTGATCCCCGTCTCAACTTTCTGAGCCAACAACCGGAATTTGTGTTGGATTCTCCATACTTTGAGACTGGAACTGCAATCCATGACCACATTGAATTGAGTGATGGTTTGGATAGAAGAAGTGAAGGGAAAGCTGGGATTTTTGGACTGCATGATGTGGAATCAGGGTCTGGAGTTCAATCATCTTCCTCCAGAAGTGAACAAAATCTTATGGGTAAAGCTGTTGAAAACAATTTCCAAGAAATTACCTCACCTAGCTCAGAATGTCATGACAAGGGTGAACTCCTTATACTGCCTTCTTCAGAAAGACCTTCTCCACCTGAAGAAAAACAGGTTCAAAATGGTAACAATTTTTTCGATGCTGCTGAAGATAGAAAAGTTGATGAGGGCAATAGTGCTGAACGCAAGAGTAAAATGTTTGATTTGGGAATTCAACAAGATGATGATGATGCTTCTGGTAGCCAGCAGCAAGGAAATGGTCTGTCTAGGAAAGAATCAGCTGGAGATTTGCTTCTGAATCTTCCAAGGATTGCTTCTCTGCCACATTTTCTGTTTCCTATGTCCGAGGATTCTGATAGTCATGTTAGATAG
Protein sequence

>MsG0480018227.01.T01

MVQLMRSDSDNNHFKKSNWNLNKMMIINFILTSVPNSISTLILLSFAAAAADSKLKASNFPATVLKIGTWELVCICCSWQGGPCFSGRSIHNQGSIPCGNQQQTLLVDKPAYIGINKYLSKLSVISSATVQCPQGLLGRHFEKLIQCDPRLNFLSQQPEFVLDSPYFETGTAIHDHIELSDGLDRRSEGKAGIFGLHDVESGSGVQSSSSRSEQNLMGKAVENNFQEITSPSSECHDKGELLILPSSERPSPPEEKQVQNGNNFFDAAEDRKVDEGNSAERKSKMFDLGIQQDDDDASGSQQQGNGLSRKESAGDLLLNLPRIASLPHFLFPMSEDSDSHVR*