AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018248.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018248.01.T01 MTR_4g008070 78.378 111 9 1 93 203 1 96 4.76e-59 181
MsG0480018248.01.T01 MTR_4g008070 93.182 44 3 0 1 44 1 44 1.35e-20 83.2
MsG0480018248.01.T01 MTR_3g100050 51.408 142 53 4 35 171 104 234 2.34e-33 122
MsG0480018248.01.T01 MTR_5g066510 52.846 123 44 4 52 171 126 237 2.35e-30 113
MsG0480018248.01.T01 MTR_3g100040 51.923 104 41 3 70 171 36 132 8.06e-28 106
MsG0480018248.01.T01 MTR_3g100040 51.923 104 41 3 70 171 140 236 1.70e-27 107
MsG0480018248.01.T01 MTR_4g093730 51.961 102 42 2 70 171 117 211 6.41e-26 102
MsG0480018248.01.T01 MTR_5g066510 50.000 80 29 3 52 131 126 194 7.51e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018248.01.T01 AT1G51600 65.686 102 35 0 70 171 156 257 1.59e-42 145
MsG0480018248.01.T01 AT1G51600 65.686 102 35 0 70 171 156 257 1.59e-42 145
MsG0480018248.01.T01 AT3G21175 59.804 102 41 0 70 171 152 253 1.65e-38 135
MsG0480018248.01.T01 AT3G21175 59.804 102 41 0 70 171 150 251 1.74e-38 135
MsG0480018248.01.T01 AT4G24470 55.660 106 30 4 72 171 154 248 8.32e-30 112
MsG0480018248.01.T01 AT4G24470 55.660 106 30 4 72 171 154 248 8.49e-30 112
MsG0480018248.01.T01 AT4G24470 55.660 106 30 4 72 171 154 248 8.49e-30 112
MsG0480018248.01.T01 AT3G21175 59.615 52 21 0 70 121 150 201 3.30e-13 66.6

Find 56 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAGGGTTCTTGGGCCTTC+AGG 0.235694 4:+2197405 None:intergenic
TGGACTTCTGTGGGCAAATA+AGG 0.252080 4:-2197373 MsG0480018248.01.T01:intron
CAAGCATTGCAGAGGGTTCT+TGG 0.293230 4:+2197396 None:intergenic
TTCTGTTCGTAGCCATTGAC+TGG 0.328675 4:-2196814 MsG0480018248.01.T01:intron
TTTAGGATGCAAAGAAATAA+AGG 0.333460 4:-2197771 MsG0480018248.01.T01:intron
TTTAGGATGCAAAGAAATAA+AGG 0.333460 4:-2200187 None:intergenic
TATATGCAAAAGTTGTTAGT+TGG 0.361179 4:-2196714 MsG0480018248.01.T01:CDS
CGGAACAAGTGAAGGTTTGA+TGG 0.376343 4:-2197691 MsG0480018248.01.T01:CDS
CGGAACAAGTGAAGGTTTGA+TGG 0.376343 4:-2200107 MsG0480018248.01.T01:CDS
ATATGCAAAAGTTGTTAGTT+GGG 0.418267 4:-2196713 MsG0480018248.01.T01:CDS
AAGCATTGCAGAGGGTTCTT+GGG 0.424963 4:+2197397 None:intergenic
TTACACATTATCTTGCTGTT+GGG 0.431240 4:+2200069 None:intergenic
TTCCTGCAAATCTGAAAAGA+AGG 0.433348 4:+2196777 None:intergenic
CTTACACATTATCCTGCTGT+TGG 0.456856 4:+2197652 None:intergenic
CTTACACATTATCTTGCTGT+TGG 0.457624 4:+2200068 None:intergenic
AGACTCATCATGGTTGGACT+TGG 0.463495 4:+2197734 None:intergenic
TGGACTTGGCAGATGTAAAT+CGG 0.465549 4:+2197748 None:intergenic
TGGACTTGGCAGATGTAAAT+CGG 0.465549 4:+2200164 None:intergenic
TTACACATTATCCTGCTGTT+GGG 0.481883 4:+2197653 None:intergenic
AGACTCGTCAAGGTTGGACT+TGG 0.483851 4:+2199024 None:intergenic
AGACTCGTCATGGTTGGACT+TGG 0.484402 4:+2200150 None:intergenic
TCAGGTCCACGCCGCATCGT+TGG 0.497989 4:+2197423 None:intergenic
CAGCCGATGCAGACTCATCA+TGG 0.498772 4:+2197724 None:intergenic
TGGACTTGGAAGATGTAAAT+CGG 0.500050 4:+2199038 None:intergenic
TTTAGGATGCAAAGAAGTAA+AGG 0.501385 4:-2199061 MsG0480018248.01.T01:intron
AATGGGAGGTAGTAGAAGAC+TGG 0.501501 4:-2196753 MsG0480018248.01.T01:CDS
CAATGCTTGTGGACTTCTGT+GGG 0.512002 4:-2197382 MsG0480018248.01.T01:CDS
GCAATGCTTGTGGACTTCTG+TGG 0.515681 4:-2197383 MsG0480018248.01.T01:CDS
TTCGTAGAGAGGTAGCATTA+AGG 0.536977 4:-2198934 MsG0480018248.01.T01:intron
AGAACCCTCTGCAATGCTTG+TGG 0.537519 4:-2197393 MsG0480018248.01.T01:CDS
AAGACTGGGCGTTCAACCTA+AGG 0.539804 4:-2196738 MsG0480018248.01.T01:CDS
ATGAATTGCGGAACAAGTGA+AGG 0.546961 4:-2197699 MsG0480018248.01.T01:CDS
ATGAATTGCGGAACAAGTGA+AGG 0.546961 4:-2200115 MsG0480018248.01.T01:CDS
AATGAGTTGCGAAACGCGGA+AGG 0.558566 4:-2198990 MsG0480018248.01.T01:CDS
CAGCCGATGCAGACTCGTCA+TGG 0.558934 4:+2200140 None:intergenic
TCTGTTCGTAGCCATTGACT+GGG 0.562926 4:-2196813 MsG0480018248.01.T01:intron
TGAAACATAAGCCCAGTCAA+TGG 0.568944 4:+2196802 None:intergenic
TGATGCAGACTCGTCAAGGT+TGG 0.569658 4:+2199018 None:intergenic
CAGCTGATGCAGACTCGTCA+AGG 0.577397 4:+2199014 None:intergenic
ACGATGCGGCGTGGACCTGA+AGG 0.583947 4:-2197420 MsG0480018248.01.T01:CDS
CAACGATGGATCCCAACAGC+AGG 0.584413 4:-2197664 MsG0480018248.01.T01:CDS
CGATGCAGACTCATCATGGT+TGG 0.584481 4:+2197728 None:intergenic
CAACCATGACGAGTCTGCAT+CGG 0.589195 4:-2200143 MsG0480018248.01.T01:CDS
CGATGCAGACTCGTCATGGT+TGG 0.592233 4:+2200144 None:intergenic
CTGAAATGAGTTGCGAAACG+CGG 0.599299 4:-2198994 MsG0480018248.01.T01:CDS
CAACCATGATGAGTCTGCAT+CGG 0.601385 4:-2197727 MsG0480018248.01.T01:CDS
TCGGTATAATGTTCGTAGAG+AGG 0.616329 4:-2198945 MsG0480018248.01.T01:CDS
GGTTTGATGGCAGACAATGA+TGG 0.622019 4:-2200094 MsG0480018248.01.T01:CDS
ATGGGAGGTAGTAGAAGACT+GGG 0.636419 4:-2196752 MsG0480018248.01.T01:CDS
GCATCGGCTGAAATGAATTG+CGG 0.643791 4:-2197711 MsG0480018248.01.T01:CDS
GCATCGGCTGAAATGAATTG+CGG 0.643791 4:-2200127 MsG0480018248.01.T01:CDS
TGCACGCCAACGATGCGGCG+TGG 0.646226 4:-2197429 MsG0480018248.01.T01:CDS
GAAGTCCACAAGCATTGCAG+AGG 0.650799 4:+2197388 None:intergenic
AGAAGTGCACGCCAACGATG+CGG 0.657729 4:-2197434 MsG0480018248.01.T01:CDS
AAGTCCACAAGCATTGCAGA+GGG 0.669436 4:+2197389 None:intergenic
GGTTTGATGGCAGACAACGA+TGG 0.718774 4:-2197678 MsG0480018248.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAATTGTATAAGAAAA+TGG - Chr4:2198769-2198788 MsG0480018248.01.T01:intron 10.0%
!! CACATATAATAAAATAACTT+CGG + Chr4:2197754-2197773 None:intergenic 15.0%
!! CCATTTATCAAATAATATAA+AGG + Chr4:2199366-2199385 None:intergenic 15.0%
!! CCTTTATATTATTTGATAAA+TGG - Chr4:2199363-2199382 MsG0480018248.01.T01:intron 15.0%
!! GTATTCTAATCTATATATTA+TGG - Chr4:2199278-2199297 MsG0480018248.01.T01:intron 15.0%
!!! AAAATTTTGACAAAAAAGTT+CGG - Chr4:2197901-2197920 MsG0480018248.01.T01:intron 15.0%
!!! TAAAAACTAAAAGTGTTTTT+AGG + Chr4:2198578-2198597 None:intergenic 15.0%
!! ACTATATATTGTTTCAGTTT+CGG - Chr4:2198397-2198416 MsG0480018248.01.T01:intron 20.0%
!! ATAACTTTGCAAATTTAAAG+TGG - Chr4:2197285-2197304 MsG0480018248.01.T01:intron 20.0%
!! ATATCATTCTTTATGGAATA+AGG + Chr4:2197267-2197286 None:intergenic 20.0%
!! CAAAGTTATATCATTCTTTA+TGG + Chr4:2197274-2197293 None:intergenic 20.0%
!! CACATAAAACAAAATAATGT+CGG + Chr4:2199044-2199063 None:intergenic 20.0%
!! CATAAAGAATGATATAACTT+TGG - Chr4:2197272-2197291 MsG0480018248.01.T01:intron 20.0%
!! TAAGAAATTGTAAACGAAAA+TGG - Chr4:2197472-2197491 MsG0480018248.01.T01:intron 20.0%
!! TAGAAGATTATCATTACATA+TGG + Chr4:2198899-2198918 None:intergenic 20.0%
!!! ATAACTTTGGAAATTTAAAG+TGG - Chr4:2198520-2198539 MsG0480018248.01.T01:intron 20.0%
!!! ATAGATATTTGTTTCTTTTG+GGG - Chr4:2198342-2198361 MsG0480018248.01.T01:intron 20.0%
!!! CTTGAAAATAAAGTCTTATT+TGG - Chr4:2199313-2199332 MsG0480018248.01.T01:intron 20.0%
!!! GATAGATATTTGCTTTTAAA+AGG - Chr4:2197081-2197100 MsG0480018248.01.T01:intron 20.0%
!!! GATAGATATTTGTTTCTTTT+GGG - Chr4:2198341-2198360 MsG0480018248.01.T01:intron 20.0%
!!! TAACTTTGCAAATTTAAAGT+GGG - Chr4:2197286-2197305 MsG0480018248.01.T01:intron 20.0%
!!! TAACTTTGGAAATTTAAAGT+GGG - Chr4:2198521-2198540 MsG0480018248.01.T01:intron 20.0%
!!! TGATAGATATTTGTTTCTTT+TGG - Chr4:2198340-2198359 MsG0480018248.01.T01:intron 20.0%
!!! TTAATTTTGAATTCCTAACA+AGG - Chr4:2197347-2197366 MsG0480018248.01.T01:intron 20.0%
! AAAAATAAGCCAATCCAAAA+GGG + Chr4:2198099-2198118 None:intergenic 25.0%
! AAACAAATATCTATCATTGC+AGG + Chr4:2198336-2198355 None:intergenic 25.0%
! AATTGTATAAGAAAATGGCA+AGG - Chr4:2198774-2198793 MsG0480018248.01.T01:intron 25.0%
! ATAAAGGACAATTTCAGTAT+AGG + Chr4:2199350-2199369 None:intergenic 25.0%
! ATAATTGTATGAAGCAACAT+TGG + Chr4:2199569-2199588 None:intergenic 25.0%
! ATATCATTCTTTATGGCATA+AGG + Chr4:2198502-2198521 None:intergenic 25.0%
! ATATGCAAAAGTTGTTAGTT+GGG - Chr4:2200152-2200171 MsG0480018248.01.T01:CDS 25.0%
! ATTTCAGTTCATATTAGTCA+AGG - Chr4:2197177-2197196 MsG0480018248.01.T01:intron 25.0%
! CAAATACAAGTAACTTCTTT+AGG - Chr4:2199077-2199096 MsG0480018248.01.T01:intron 25.0%
! GAGAAGATAAAAAACAAACT+TGG - Chr4:2197406-2197425 MsG0480018248.01.T01:CDS 25.0%
! GAGAAGATTATCATTACATA+CGG + Chr4:2197612-2197631 None:intergenic 25.0%
! TATATGCAAAAGTTGTTAGT+TGG - Chr4:2200151-2200170 MsG0480018248.01.T01:CDS 25.0%
! TTGCTTCATACAATTATTAC+AGG - Chr4:2199572-2199591 MsG0480018248.01.T01:intron 25.0%
!! AACTTTGCAAATTTAAAGTG+GGG - Chr4:2197287-2197306 MsG0480018248.01.T01:intron 25.0%
!! AACTTTGGAAATTTAAAGTG+GGG - Chr4:2198522-2198541 MsG0480018248.01.T01:intron 25.0%
!! TATCATTACATATGGTGTTA+AGG + Chr4:2198891-2198910 None:intergenic 25.0%
!! TTACAGGTTAATCATGATTT+TGG - Chr4:2199588-2199607 MsG0480018248.01.T01:intron 25.0%
!! TTTAGGATGCAAAGAAATAA+AGG - Chr4:2199094-2199113 MsG0480018248.01.T01:intron 25.0%
!! TTTGAAGTAAAAATGTAAGC+AGG - Chr4:2197534-2197553 MsG0480018248.01.T01:intron 25.0%
!!! ACTGGTTTATTTTTGAACAA+GGG - Chr4:2196823-2196842 MsG0480018248.01.T01:intron 25.0%
!!! AGATATTTGCTTTTAAAAGG+AGG - Chr4:2197084-2197103 MsG0480018248.01.T01:intron 25.0%
!!! ATGTTTTTATTAATTCCACC+TGG - Chr4:2199862-2199881 MsG0480018248.01.T01:intron 25.0%
!!! TATATCAGTTAAGCTTTAGA+TGG + Chr4:2197228-2197247 None:intergenic 25.0%
AAATATCTATCATTGCAGAC+TGG + Chr4:2197072-2197091 None:intergenic 30.0%
AAATGTGAAGATGCATAAAC+AGG + Chr4:2199966-2199985 None:intergenic 30.0%
AATGTGAAGATGCATAAACA+GGG + Chr4:2199965-2199984 None:intergenic 30.0%
AATTGTAAACGAAAATGGTG+AGG - Chr4:2197477-2197496 MsG0480018248.01.T01:intron 30.0%
ACAATGACAGATAAATGAAG+GGG + Chr4:2199688-2199707 None:intergenic 30.0%
AGAGAAGATAGAAACAAACT+AGG - Chr4:2198672-2198691 MsG0480018248.01.T01:intron 30.0%
AGCAACATTGGAAAAACAAA+AGG + Chr4:2199557-2199576 None:intergenic 30.0%
AGCATTAAGGTATGTTTAGA+GGG - Chr4:2197944-2197963 MsG0480018248.01.T01:intron 30.0%
ATTGTAAACGAAAATGGTGA+GGG - Chr4:2197478-2197497 MsG0480018248.01.T01:intron 30.0%
CAAAAATAAGCCAATCCAAA+AGG + Chr4:2198100-2198119 None:intergenic 30.0%
CAAATACAAGCAACTTCTTT+AGG - Chr4:2197787-2197806 MsG0480018248.01.T01:intron 30.0%
CACAATGACAGATAAATGAA+GGG + Chr4:2199689-2199708 None:intergenic 30.0%
CCTGTAATATTAAGCCAATT+CGG - Chr4:2199613-2199632 MsG0480018248.01.T01:intron 30.0%
GTTCATATTAGTTAAGGACT+TGG - Chr4:2198419-2198438 MsG0480018248.01.T01:intron 30.0%
GTTGAAGTAAAAATGAAAGC+AGG - Chr4:2198831-2198850 MsG0480018248.01.T01:intron 30.0%
GTTTCGGTTCATATTAGTTA+AGG - Chr4:2198413-2198432 MsG0480018248.01.T01:intron 30.0%
TAGCATTAAGGTATGTTTAG+AGG - Chr4:2197943-2197962 MsG0480018248.01.T01:intron 30.0%
TATGATTCATAAGCTTGTCA+AGG - Chr4:2196893-2196912 MsG0480018248.01.T01:intron 30.0%
TCACAATGACAGATAAATGA+AGG + Chr4:2199690-2199709 None:intergenic 30.0%
TCATTGTGAATGTGTTAAAG+TGG - Chr4:2199701-2199720 MsG0480018248.01.T01:intron 30.0%
TTCAGATTTGCAGGAAAAAT+GGG - Chr4:2200095-2200114 MsG0480018248.01.T01:CDS 30.0%
TTTCAACTCATATCAGTTGT+AGG - Chr4:2199392-2199411 MsG0480018248.01.T01:intron 30.0%
TTTCAGATTTGCAGGAAAAA+TGG - Chr4:2200094-2200113 MsG0480018248.01.T01:CDS 30.0%
! AAGATAATGTGTAAGTTGAC+TGG - Chr4:2196805-2196824 MsG0480018248.01.T01:CDS 30.0%
! ATATTAAGCCAATTCGGTTT+TGG - Chr4:2199619-2199638 MsG0480018248.01.T01:intron 30.0%
! CAACTTTTGCATATAACCTT+AGG + Chr4:2200146-2200165 None:intergenic 30.0%
! CGAATTGGCTTAATATTACA+GGG + Chr4:2199615-2199634 None:intergenic 30.0%
! TTTAGGATGCAAAGAAGTAA+AGG - Chr4:2197804-2197823 MsG0480018248.01.T01:intron 30.0%
!! ACTATTACACTGCTTTAAGA+AGG + Chr4:2197642-2197661 None:intergenic 30.0%
!! CTATTACACTGCTTTAAGAA+GGG + Chr4:2197641-2197660 None:intergenic 30.0%
!! TATATCAGCTAAGCTTTAGA+TGG + Chr4:2198463-2198482 None:intergenic 30.0%
!! TCTTTTGGGAAATAGTATTC+AGG - Chr4:2197120-2197139 MsG0480018248.01.T01:intron 30.0%
!! TTACACATTATCTTGCTGTT+GGG + Chr4:2196799-2196818 None:intergenic 30.0%
!!! GACTGGTTTATTTTTGAACA+AGG - Chr4:2196822-2196841 MsG0480018248.01.T01:intron 30.0%
AAAAGGGTCAATGTTCTGTT+TGG + Chr4:2198083-2198102 None:intergenic 35.0%
AAATAATGTCGGTCAGAACA+TGG + Chr4:2199033-2199052 None:intergenic 35.0%
AATCCAAACAAGTCCATAGT+TGG - Chr4:2197035-2197054 MsG0480018248.01.T01:intron 35.0%
ACAATGAAGGAGAAGATGAA+TGG + Chr4:2199920-2199939 None:intergenic 35.0%
ACTCAGCATATAAACTTGAC+AGG + Chr4:2198975-2198994 None:intergenic 35.0%
ATCACATATGTTTGCGACAT+AGG + Chr4:2198650-2198669 None:intergenic 35.0%
CTATTACACTGCCTTAAGAA+AGG + Chr4:2198928-2198947 None:intergenic 35.0%
GAAAATGGCAAGGAAAATGT+TGG - Chr4:2198784-2198803 MsG0480018248.01.T01:intron 35.0%
GAAAATGGTGAGGGAAATAT+TGG - Chr4:2197487-2197506 MsG0480018248.01.T01:intron 35.0%
GATAAATGAAGGGGAAAAAG+TGG + Chr4:2199679-2199698 None:intergenic 35.0%
GGGGATTTGATGAATACATT+TGG - Chr4:2197306-2197325 MsG0480018248.01.T01:intron 35.0%
GTTCATATTAGTCAAGGACT+TGG - Chr4:2197183-2197202 MsG0480018248.01.T01:intron 35.0%
TCATCTTCTACTGAATCGTA+TGG - Chr4:2200014-2200033 MsG0480018248.01.T01:intron 35.0%
TGGACTTGGAAGATGTAAAT+CGG + Chr4:2197830-2197849 None:intergenic 35.0%
TGTGGGCAAATAAGGTAAAT+GGG - Chr4:2199500-2199519 MsG0480018248.01.T01:intron 35.0%
TTACACATTATCCTGCTGTT+GGG + Chr4:2199215-2199234 None:intergenic 35.0%
TTCCTGCAAATCTGAAAAGA+AGG + Chr4:2200091-2200110 None:intergenic 35.0%
TTGAATTCCTAACAAGGAGA+TGG - Chr4:2197353-2197372 MsG0480018248.01.T01:intron 35.0%
! AAACAGAACATTGACCCTTT+TGG - Chr4:2198082-2198101 MsG0480018248.01.T01:intron 35.0%
! AACTATGGACTTGTTTGGAT+TGG + Chr4:2197036-2197055 None:intergenic 35.0%
! ATAAGTTTTGCGAGACTCTT+TGG + Chr4:2198032-2198051 None:intergenic 35.0%
! CCGAATTGGCTTAATATTAC+AGG + Chr4:2199616-2199635 None:intergenic 35.0%
! CTTACACATTATCTTGCTGT+TGG + Chr4:2196800-2196819 None:intergenic 35.0%
! GTTAAAGTGGCATACTGATT+TGG - Chr4:2199714-2199733 MsG0480018248.01.T01:intron 35.0%
AAGTCTTCCATCTCCTTGTT+AGG + Chr4:2197363-2197382 None:intergenic 40.0%
ACTCAGCAGATAAACTTGAC+AGG + Chr4:2197688-2197707 None:intergenic 40.0%
AGATTTGCAGGAAAAATGGG+AGG - Chr4:2200098-2200117 MsG0480018248.01.T01:CDS 40.0%
AGGATAATGTGTAAGCAGAC+TGG - Chr4:2199221-2199240 MsG0480018248.01.T01:intron 40.0%
ATGAATTGCGGAACAAGTGA+AGG - Chr4:2196750-2196769 MsG0480018248.01.T01:CDS 40.0%
ATGGTCAAACCTAAACCAAG+AGG + Chr4:2199901-2199920 None:intergenic 40.0%
CAATCCAAACGAGTTCATAG+TGG - Chr4:2198299-2198318 MsG0480018248.01.T01:intron 40.0%
CTGTGGGCAAATAAGGTAAA+TGG - Chr4:2199499-2199518 MsG0480018248.01.T01:intron 40.0%
CTTACACATTATCCTGCTGT+TGG + Chr4:2199216-2199235 None:intergenic 40.0%
CTTCTTCTTCCTCTTGGTTT+AGG - Chr4:2199889-2199908 MsG0480018248.01.T01:intron 40.0%
GAGATGCGAAGAAACAATGA+AGG + Chr4:2199933-2199952 None:intergenic 40.0%
TATACGGACCAAAACCGAAT+TGG + Chr4:2199630-2199649 None:intergenic 40.0%
TCGGTATAATGTTCGTAGAG+AGG - Chr4:2197920-2197939 MsG0480018248.01.T01:intron 40.0%
TGAAACATAAGCCCAGTCAA+TGG + Chr4:2200066-2200085 None:intergenic 40.0%
TGGACTTGGCAGATGTAAAT+CGG + Chr4:2196704-2196723 None:intergenic 40.0%
TTCGTAGAGAGGTAGCATTA+AGG - Chr4:2197931-2197950 MsG0480018248.01.T01:intron 40.0%
! AGAACTCCATTGGAGTGTTA+GGG + Chr4:2197580-2197599 None:intergenic 40.0%
! GAACATTGACCCTTTTGGAT+TGG - Chr4:2198087-2198106 MsG0480018248.01.T01:intron 40.0%
! GCTCACTCAGTGTTTCTTTT+GGG - Chr4:2197106-2197125 MsG0480018248.01.T01:intron 40.0%
! TGCCTTCTTTTCAGATTTGC+AGG - Chr4:2200086-2200105 MsG0480018248.01.T01:CDS 40.0%
!! CTTTTGGGGAAATAGTGTTC+TGG - Chr4:2198356-2198375 MsG0480018248.01.T01:intron 40.0%
!! TTCTAGCTCTGCCTTTCTTA+AGG - Chr4:2198914-2198933 MsG0480018248.01.T01:intron 40.0%
AACTGACCCTAACACTCCAA+TGG - Chr4:2197571-2197590 MsG0480018248.01.T01:intron 45.0%
AATGGGAGGTAGTAGAAGAC+TGG - Chr4:2200112-2200131 MsG0480018248.01.T01:CDS 45.0%
ACTGCCACTATGAACTCGTT+TGG + Chr4:2198306-2198325 None:intergenic 45.0%
AGACTCATCATGGTTGGACT+TGG + Chr4:2199134-2199153 None:intergenic 45.0%
AGAGGAAGAAGAAGAATGCC+AGG + Chr4:2199883-2199902 None:intergenic 45.0%
ATGGGAGGTAGTAGAAGACT+GGG - Chr4:2200113-2200132 MsG0480018248.01.T01:CDS 45.0%
CAACCATGATGAGTCTGCAT+CGG - Chr4:2199138-2199157 MsG0480018248.01.T01:intron 45.0%
CAATGCTTGTGGACTTCTGT+GGG - Chr4:2199483-2199502 MsG0480018248.01.T01:intron 45.0%
CGGAACAAGTGAAGGTTTGA+TGG - Chr4:2196758-2196777 MsG0480018248.01.T01:CDS 45.0%
CTGAAATGAGTTGCGAAACG+CGG - Chr4:2197871-2197890 MsG0480018248.01.T01:intron 45.0%
GAGCTTATGCGAAGCAACTT+AGG - Chr4:2198119-2198138 MsG0480018248.01.T01:intron 45.0%
GGCATTCTTCTTCTTCCTCT+TGG - Chr4:2199883-2199902 MsG0480018248.01.T01:intron 45.0%
GGTCAATGAACTGCCAACTA+TGG + Chr4:2197051-2197070 None:intergenic 45.0%
GTGTTAAGGCAGCAGTGTTA+GGG + Chr4:2198877-2198896 None:intergenic 45.0%
GTGTTCAGACAGAACTCCAT+TGG + Chr4:2197590-2197609 None:intergenic 45.0%
TGGACTTCTGTGGGCAAATA+AGG - Chr4:2199492-2199511 MsG0480018248.01.T01:intron 45.0%
TTCTGTTCGTAGCCATTGAC+TGG - Chr4:2200051-2200070 MsG0480018248.01.T01:intron 45.0%
! AAGCATTGCAGAGGGTTCTT+GGG + Chr4:2199471-2199490 None:intergenic 45.0%
! AAGTCCACAAGCATTGCAGA+GGG + Chr4:2199479-2199498 None:intergenic 45.0%
! CAGAACTCCATTGGAGTGTT+AGG + Chr4:2197581-2197600 None:intergenic 45.0%
! CTGCCAACTATGGACTTGTT+TGG + Chr4:2197041-2197060 None:intergenic 45.0%
! GGCTCACTCAGTGTTTCTTT+TGG - Chr4:2197105-2197124 MsG0480018248.01.T01:intron 45.0%
! GGTTTGATGGCAGACAATGA+TGG - Chr4:2196771-2196790 MsG0480018248.01.T01:CDS 45.0%
! TCTGTTCGTAGCCATTGACT+GGG - Chr4:2200052-2200071 MsG0480018248.01.T01:intron 45.0%
!! GCATCGGCTGAAATGAATTG+CGG - Chr4:2196738-2196757 MsG0480018248.01.T01:CDS 45.0%
!!! TCTAAATTTTTTATTTTATA+CGG + Chr4:2199646-2199665 None:intergenic 5.0%
!!! TTAATTTTAAATTACTAATA+AGG - Chr4:2198614-2198633 MsG0480018248.01.T01:intron 5.0%
AAGACTGGGCGTTCAACCTA+AGG - Chr4:2200127-2200146 MsG0480018248.01.T01:CDS 50.0%
AATGAGTTGCGAAACGCGGA+AGG - Chr4:2197875-2197894 MsG0480018248.01.T01:intron 50.0%
AGAACCCTCTGCAATGCTTG+TGG - Chr4:2199472-2199491 MsG0480018248.01.T01:intron 50.0%
AGACTCGTCAAGGTTGGACT+TGG + Chr4:2197844-2197863 None:intergenic 50.0%
AGACTCGTCATGGTTGGACT+TGG + Chr4:2196718-2196737 None:intergenic 50.0%
CAACCATGACGAGTCTGCAT+CGG - Chr4:2196722-2196741 MsG0480018248.01.T01:CDS 50.0%
CGATGCAGACTCATCATGGT+TGG + Chr4:2199140-2199159 None:intergenic 50.0%
GCAATGCTTGTGGACTTCTG+TGG - Chr4:2199482-2199501 MsG0480018248.01.T01:intron 50.0%
GGAAGAAGAAGAATGCCAGG+TGG + Chr4:2199880-2199899 None:intergenic 50.0%
GGTGTTAAGGCAGCAGTGTT+AGG + Chr4:2198878-2198897 None:intergenic 50.0%
TGATGCAGACTCGTCAAGGT+TGG + Chr4:2197850-2197869 None:intergenic 50.0%
! CAAGCATTGCAGAGGGTTCT+TGG + Chr4:2199472-2199491 None:intergenic 50.0%
! GAAGTCCACAAGCATTGCAG+AGG + Chr4:2199480-2199499 None:intergenic 50.0%
! GGTTTGATGGCAGACAACGA+TGG - Chr4:2199187-2199206 MsG0480018248.01.T01:intron 50.0%
CAACGATGGATCCCAACAGC+AGG - Chr4:2199201-2199220 MsG0480018248.01.T01:intron 55.0%
CAGCCGATGCAGACTCATCA+TGG + Chr4:2199144-2199163 None:intergenic 55.0%
CAGCTGATGCAGACTCGTCA+AGG + Chr4:2197854-2197873 None:intergenic 55.0%
CGATGCAGACTCGTCATGGT+TGG + Chr4:2196724-2196743 None:intergenic 55.0%
! AGAAGTGCACGCCAACGATG+CGG - Chr4:2199431-2199450 MsG0480018248.01.T01:intron 55.0%
CAGAGGGTTCTTGGGCCTTC+AGG + Chr4:2199463-2199482 None:intergenic 60.0%
CAGCCGATGCAGACTCGTCA+TGG + Chr4:2196728-2196747 None:intergenic 60.0%
ACGATGCGGCGTGGACCTGA+AGG - Chr4:2199445-2199464 MsG0480018248.01.T01:intron 65.0%
TCAGGTCCACGCCGCATCGT+TGG + Chr4:2199445-2199464 None:intergenic 65.0%
TGCACGCCAACGATGCGGCG+TGG - Chr4:2199436-2199455 MsG0480018248.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 2196684 2200203 2196684 ID=MsG0480018248.01;Name=MsG0480018248.01
Chr4 mRNA 2196684 2200203 2196684 ID=MsG0480018248.01.T01;Parent=MsG0480018248.01;Name=MsG0480018248.01.T01;_AED=0.58;_eAED=0.61;_QI=0|0|0|0.8|1|1|5|0|217
Chr4 exon 2200073 2200203 2200073 ID=MsG0480018248.01.T01:exon:1130;Parent=MsG0480018248.01.T01
Chr4 exon 2198935 2199078 2198935 ID=MsG0480018248.01.T01:exon:1129;Parent=MsG0480018248.01.T01
Chr4 exon 2197657 2197788 2197657 ID=MsG0480018248.01.T01:exon:1128;Parent=MsG0480018248.01.T01
Chr4 exon 2197374 2197479 2197374 ID=MsG0480018248.01.T01:exon:1127;Parent=MsG0480018248.01.T01
Chr4 exon 2196684 2196824 2196684 ID=MsG0480018248.01.T01:exon:1126;Parent=MsG0480018248.01.T01
Chr4 CDS 2200073 2200203 2200073 ID=MsG0480018248.01.T01:cds;Parent=MsG0480018248.01.T01
Chr4 CDS 2198935 2199078 2198935 ID=MsG0480018248.01.T01:cds;Parent=MsG0480018248.01.T01
Chr4 CDS 2197657 2197788 2197657 ID=MsG0480018248.01.T01:cds;Parent=MsG0480018248.01.T01
Chr4 CDS 2197374 2197479 2197374 ID=MsG0480018248.01.T01:cds;Parent=MsG0480018248.01.T01
Chr4 CDS 2196684 2196824 2196684 ID=MsG0480018248.01.T01:cds;Parent=MsG0480018248.01.T01
Gene Sequence

>MsG0480018248.01.T01

ATGCAAAGAAATAAAGGCCGATTTACATCTGCCAAGTCCAACCATGACGAGTCTGCATCGGCTGAAATGAATTGCGGAACAAGTGAAGGTTTGATGGCAGACAATGATGGATCCCAACAGCAAGATAATGTGATGCAAAGAAGTAAAGGCCGATTTACATCTTCCAAGTCCAACCTTGACGAGTCTGCATCAGCTGAAATGAGTTGCGAAACGCGGAAGGAACAAAATTTTGACAAAAAAGTTCGGTATAATGTTCGTAGAGAGGTAGCATTAAGGATGCAAAGAAATAAAGGCCGATTTACATCTGCCAAGTCCAACCATGATGAGTCTGCATCGGCTGAAATGAATTGCGGAACAAGTGAAGGTTTGATGGCAGACAACGATGGATCCCAACAGCAGGATAATGTTTGTAGGCATTGTAACATCAGTGAGAAGTGCACGCCAACGATGCGGCGTGGACCTGAAGGCCCAAGAACCCTCTGCAATGCTTGTGGACTTCTGTGGGCAAATAAGCCATTGACTGGGCTTATGTTTCATGCCTTCTTTTCAGATTTGCAGGAAAAATGGGAGGTAGTAGAAGACTGGGCGTTCAACCTAAGGTTATATGCAAAAGTTGTTAGTTGGGATGATGAATTTGACGTTGTTGATGAATAA
Protein sequence

>MsG0480018248.01.T01

MQRNKGRFTSAKSNHDESASAEMNCGTSEGLMADNDGSQQQDNVMQRSKGRFTSSKSNLDESASAEMSCETRKEQNFDKKVRYNVRREVALRMQRNKGRFTSAKSNHDESASAEMNCGTSEGLMADNDGSQQQDNVCRHCNISEKCTPTMRRGPEGPRTLCNACGLLWANKPLTGLMFHAFFSDLQEKWEVVEDWAFNLRLYAKVVSWDDEFDVVDE*