Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018398.01.T01 | XP_013454680.1 | 93.939 | 165 | 9 | 1 | 1 | 165 | 1 | 164 | 1.27E-94 | 281 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018398.01.T01 | O49816 | 72.571 | 175 | 25 | 2 | 1 | 153 | 1 | 174 | 4.39E-74 | 222 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018398.01.T01 | A0A072UH01 | 93.939 | 165 | 9 | 1 | 1 | 165 | 1 | 164 | 6.04e-95 | 281 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380017515.01 | MsG0480018398.01 | 0.802996 | 4.303137e-49 | 2.137945e-46 |
| MsG0480018398.01 | MsG0880047296.01 | 0.804074 | 2.572618e-49 | 1.314139e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018398.01.T01 | MTR_4g011230 | 93.939 | 165 | 9 | 1 | 1 | 165 | 1 | 164 | 1.53e-98 | 281 |
| MsG0480018398.01.T01 | MTR_4g011250 | 93.939 | 165 | 9 | 1 | 1 | 165 | 1 | 164 | 5.41e-98 | 280 |
| MsG0480018398.01.T01 | MTR_4g011270 | 70.186 | 161 | 20 | 3 | 1 | 161 | 1 | 133 | 6.34e-67 | 200 |
| MsG0480018398.01.T01 | MTR_1g045950 | 32.584 | 178 | 79 | 5 | 1 | 141 | 1 | 174 | 3.50e-14 | 67.4 |
| MsG0480018398.01.T01 | MTR_1g045980 | 34.266 | 143 | 69 | 3 | 6 | 141 | 10 | 134 | 1.29e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018398.01.T01 | AT1G52690 | 51.266 | 158 | 67 | 5 | 1 | 153 | 1 | 153 | 1.02e-38 | 130 |
| MsG0480018398.01.T01 | AT1G52690 | 51.266 | 158 | 67 | 5 | 1 | 153 | 1 | 153 | 1.02e-38 | 130 |
| MsG0480018398.01.T01 | AT3G15670 | 40.099 | 202 | 69 | 3 | 1 | 151 | 1 | 201 | 7.73e-33 | 116 |
| MsG0480018398.01.T01 | AT4G13560 | 46.392 | 97 | 48 | 1 | 46 | 142 | 15 | 107 | 6.01e-17 | 72.4 |
| MsG0480018398.01.T01 | AT4G13560 | 46.392 | 97 | 48 | 1 | 46 | 142 | 15 | 107 | 6.01e-17 | 72.4 |
Find 52 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTTGCTTGGGCTGTTTGC+TGG | 0.217726 | 4:-4361715 | None:intergenic |
| TCCTGATTGGGCAGTTTCTT+TGG | 0.242344 | 4:-4361801 | None:intergenic |
| TTACTGCTTGGGCTGTTTGC+TGG | 0.254500 | 4:-4361682 | None:intergenic |
| CTGAGTTGTCCTTTCCTGAT+TGG | 0.261169 | 4:-4361814 | None:intergenic |
| GAGGCAGTTAAGAACACTTT+AGG | 0.335169 | 4:+4361893 | MsG0480018398.01.T01:CDS |
| TACTGCTTGGGCTGTTTGCT+GGG | 0.347910 | 4:-4361681 | None:intergenic |
| TGCTTGACCAGTTGTGTCTT+GGG | 0.350466 | 4:-4361747 | None:intergenic |
| TTGCTTGACCAGTTGTGTCT+TGG | 0.350857 | 4:-4361748 | None:intergenic |
| CTGTCTTCTCCTTAGCTGCT+TGG | 0.355767 | 4:-4361628 | None:intergenic |
| TGAGTTGTCCTTTCCTGATT+GGG | 0.358541 | 4:-4361813 | None:intergenic |
| GGGCAGTTTCTTTGGTAGCC+TGG | 0.369272 | 4:-4361793 | None:intergenic |
| CACCCACATTGCCCATCATC+TGG | 0.369966 | 4:-4361565 | None:intergenic |
| TGTCTTCTCCTTAGCTGCTT+GGG | 0.380418 | 4:-4361627 | None:intergenic |
| AAGAGACAAGGGTTATGAAA+TGG | 0.391210 | 4:+4361769 | MsG0480018398.01.T01:CDS |
| TGTTGCTTGGGCTGTTTGCT+GGG | 0.405323 | 4:-4361714 | None:intergenic |
| ACAGACAGGTGAAAAGGTTA+AGG | 0.409547 | 4:+4361850 | MsG0480018398.01.T01:CDS |
| ACCAAAGAAACTGCCCAATC+AGG | 0.426421 | 4:+4361800 | MsG0480018398.01.T01:CDS |
| TCAGGAAAGGACAACTCAGC+TGG | 0.445316 | 4:+4361818 | MsG0480018398.01.T01:CDS |
| GCAAACAGCCCAAGCAGTAA+AGG | 0.474093 | 4:+4361685 | MsG0480018398.01.T01:CDS |
| AGAGACAAGGGTTATGAAAT+GGG | 0.483639 | 4:+4361770 | MsG0480018398.01.T01:CDS |
| GGCACAAGCTGCAAAGGAAA+AGG | 0.490001 | 4:+4361592 | MsG0480018398.01.T01:CDS |
| TTTGCAACAGACAGGTGAAA+AGG | 0.493254 | 4:+4361844 | MsG0480018398.01.T01:CDS |
| GGAAAAGACCAACCAGATGA+TGG | 0.497629 | 4:+4361553 | MsG0480018398.01.T01:intron |
| CAGACAGGTGAAAAGGTTAA+GGG | 0.501139 | 4:+4361851 | MsG0480018398.01.T01:CDS |
| GATAAAACCCAAGACACAAC+TGG | 0.501444 | 4:+4361740 | MsG0480018398.01.T01:CDS |
| GCAAACAGCCCAAGCAGCTA+AGG | 0.503103 | 4:+4361619 | MsG0480018398.01.T01:CDS |
| ACAGACAGCCCAAGCTGCAA+AGG | 0.520144 | 4:+4361652 | MsG0480018398.01.T01:CDS |
| AGGTGAAAAGGTTAAGGGTA+TGG | 0.530822 | 4:+4361856 | MsG0480018398.01.T01:CDS |
| CACATTGCCCATCATCTGGT+TGG | 0.536586 | 4:-4361561 | None:intergenic |
| CAAGCAGCTAAGGAGAAGAC+AGG | 0.539522 | 4:+4361629 | MsG0480018398.01.T01:CDS |
| CTACAGAGCTGGTGAGACCA+AGG | 0.542707 | 4:+4360913 | MsG0480018398.01.T01:CDS |
| TGACAAGGCACAAGCTGCAA+AGG | 0.548765 | 4:+4361586 | MsG0480018398.01.T01:CDS |
| GCAAACAGCCCAAGCAACAA+AGG | 0.560474 | 4:+4361718 | MsG0480018398.01.T01:CDS |
| GAAAAGACCAACCAGATGAT+GGG | 0.562394 | 4:+4361554 | MsG0480018398.01.T01:CDS |
| CAAGGGTTATGAAATGGGCC+AGG | 0.569377 | 4:+4361775 | MsG0480018398.01.T01:CDS |
| AGCTCTGTAGCTCTGATCGT+GGG | 0.573218 | 4:-4360900 | None:intergenic |
| CACGATCAGAGCTACAGAGC+TGG | 0.584941 | 4:+4360902 | MsG0480018398.01.T01:CDS |
| TCAATCAACAACGTTACGAC+GGG | 0.588754 | 4:-4361975 | None:intergenic |
| CAGCTCTGTAGCTCTGATCG+TGG | 0.597097 | 4:-4360901 | None:intergenic |
| CTCAATCAACAACGTTACGA+CGG | 0.597111 | 4:-4361976 | None:intergenic |
| AACTGGTCAAGCAAGAGACA+AGG | 0.604533 | 4:+4361757 | MsG0480018398.01.T01:CDS |
| CAGGAAAGGACAACTCAGCT+GGG | 0.615458 | 4:+4361819 | MsG0480018398.01.T01:CDS |
| AGAAACTGCCCAATCAGGAA+AGG | 0.617206 | 4:+4361805 | MsG0480018398.01.T01:CDS |
| AACCAGATGATGGGCAATGT+GGG | 0.622463 | 4:+4361563 | MsG0480018398.01.T01:CDS |
| TACAGAGCTGGTGAGACCAA+GGG | 0.628142 | 4:+4360914 | MsG0480018398.01.T01:CDS |
| AAGGTTAAGGGTATGGCACA+AGG | 0.628466 | 4:+4361863 | MsG0480018398.01.T01:CDS |
| TGAAAATCAAGATGAAGACA+AGG | 0.635276 | 4:+4361922 | MsG0480018398.01.T01:CDS |
| GATGGGCAATGTGGGTGACA+AGG | 0.639566 | 4:+4361571 | MsG0480018398.01.T01:CDS |
| TATGGCACAAGGTGCAACTG+AGG | 0.655239 | 4:+4361874 | MsG0480018398.01.T01:CDS |
| CAACCAGATGATGGGCAATG+TGG | 0.657668 | 4:+4361562 | MsG0480018398.01.T01:CDS |
| ACTGGTCAAGCAAGAGACAA+GGG | 0.675515 | 4:+4361758 | MsG0480018398.01.T01:CDS |
| TGAGACCAAGGGTCGAACTG+AGG | 0.699004 | 4:+4360925 | MsG0480018398.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATATATATTTTTTACAG+AGG | + | Chr4:4361203-4361222 | MsG0480018398.01.T01:intron | 10.0% |
| !!! | TTTTTTACATTTTTATTGAT+AGG | + | Chr4:4361031-4361050 | MsG0480018398.01.T01:intron | 10.0% |
| !! | AAATAAAATAAAGACTCTAT+AGG | - | Chr4:4361011-4361030 | None:intergenic | 15.0% |
| !!! | ATTTTTTTTATTAATCACCA+AGG | - | Chr4:4361466-4361485 | None:intergenic | 15.0% |
| !! | TTACTTGTATATTTACATAC+AGG | - | Chr4:4361107-4361126 | None:intergenic | 20.0% |
| !! | TTTAAATTGTTGTGACTATA+TGG | + | Chr4:4361316-4361335 | MsG0480018398.01.T01:intron | 20.0% |
| !!! | AAATATCATTTCATTTTCCT+TGG | + | Chr4:4361446-4361465 | MsG0480018398.01.T01:intron | 20.0% |
| !!! | AATTTGTTATTTGTTGGATT+AGG | + | Chr4:4361532-4361551 | MsG0480018398.01.T01:intron | 20.0% |
| !!! | ATTGTGAATTTGTTATTTGT+TGG | + | Chr4:4361526-4361545 | MsG0480018398.01.T01:intron | 20.0% |
| ! | CTATAACATAGTAGTGATTA+TGG | + | Chr4:4361362-4361381 | MsG0480018398.01.T01:intron | 25.0% |
| !!! | TTGTTGTTTTTGTTGTGAAA+TGG | - | Chr4:4361949-4361968 | None:intergenic | 25.0% |
| TGAAAATCAAGATGAAGACA+AGG | + | Chr4:4361922-4361941 | MsG0480018398.01.T01:CDS | 30.0% | |
| AAGAGACAAGGGTTATGAAA+TGG | + | Chr4:4361769-4361788 | MsG0480018398.01.T01:CDS | 35.0% | |
| AGAGACAAGGGTTATGAAAT+GGG | + | Chr4:4361770-4361789 | MsG0480018398.01.T01:CDS | 35.0% | |
| !! | AGTAGTGATTATGGTGTCAT+AGG | + | Chr4:4361371-4361390 | MsG0480018398.01.T01:intron | 35.0% |
| !! | GGTTTTATCCTTTGTTGCTT+GGG | - | Chr4:4361729-4361748 | None:intergenic | 35.0% |
| ACAGACAGGTGAAAAGGTTA+AGG | + | Chr4:4361850-4361869 | MsG0480018398.01.T01:CDS | 40.0% | |
| AGGTGAAAAGGTTAAGGGTA+TGG | + | Chr4:4361856-4361875 | MsG0480018398.01.T01:CDS | 40.0% | |
| CAGACAGGTGAAAAGGTTAA+GGG | + | Chr4:4361851-4361870 | MsG0480018398.01.T01:CDS | 40.0% | |
| GAAAAGACCAACCAGATGAT+GGG | + | Chr4:4361554-4361573 | MsG0480018398.01.T01:CDS | 40.0% | |
| GAGGCAGTTAAGAACACTTT+AGG | + | Chr4:4361893-4361912 | MsG0480018398.01.T01:CDS | 40.0% | |
| GATAAAACCCAAGACACAAC+TGG | + | Chr4:4361740-4361759 | MsG0480018398.01.T01:CDS | 40.0% | |
| TCAATCAACAACGTTACGAC+GGG | - | Chr4:4361978-4361997 | None:intergenic | 40.0% | |
| TGAGTTGTCCTTTCCTGATT+GGG | - | Chr4:4361816-4361835 | None:intergenic | 40.0% | |
| TTTGCAACAGACAGGTGAAA+AGG | + | Chr4:4361844-4361863 | MsG0480018398.01.T01:CDS | 40.0% | |
| !! | GGGTTTTATCCTTTGTTGCT+TGG | - | Chr4:4361730-4361749 | None:intergenic | 40.0% |
| !! | GGTTTTGTCCTTTACTGCTT+GGG | - | Chr4:4361696-4361715 | None:intergenic | 40.0% |
| AACTGGTCAAGCAAGAGACA+AGG | + | Chr4:4361757-4361776 | MsG0480018398.01.T01:CDS | 45.0% | |
| ACCAAAGAAACTGCCCAATC+AGG | + | Chr4:4361800-4361819 | MsG0480018398.01.T01:CDS | 45.0% | |
| ACTGGTCAAGCAAGAGACAA+GGG | + | Chr4:4361758-4361777 | MsG0480018398.01.T01:CDS | 45.0% | |
| AGAAACTGCCCAATCAGGAA+AGG | + | Chr4:4361805-4361824 | MsG0480018398.01.T01:CDS | 45.0% | |
| CTGAGTTGTCCTTTCCTGAT+TGG | - | Chr4:4361817-4361836 | None:intergenic | 45.0% | |
| GGAAAAGACCAACCAGATGA+TGG | + | Chr4:4361553-4361572 | MsG0480018398.01.T01:intron | 45.0% | |
| TCCTGATTGGGCAGTTTCTT+TGG | - | Chr4:4361804-4361823 | None:intergenic | 45.0% | |
| TGCTTGACCAGTTGTGTCTT+GGG | - | Chr4:4361750-4361769 | None:intergenic | 45.0% | |
| TGTCTTCTCCTTAGCTGCTT+GGG | - | Chr4:4361630-4361649 | None:intergenic | 45.0% | |
| TTGCTTGACCAGTTGTGTCT+TGG | - | Chr4:4361751-4361770 | None:intergenic | 45.0% | |
| ! | AAGGTTAAGGGTATGGCACA+AGG | + | Chr4:4361863-4361882 | MsG0480018398.01.T01:CDS | 45.0% |
| ! | GGTTTTCTCCTTTGCAGCTT+GGG | - | Chr4:4361663-4361682 | None:intergenic | 45.0% |
| ! | TTTTACCTCAGTTCGACCCT+TGG | - | Chr4:4360933-4360952 | None:intergenic | 45.0% |
| !! | AACCAGATGATGGGCAATGT+GGG | + | Chr4:4361563-4361582 | MsG0480018398.01.T01:CDS | 45.0% |
| !! | GGGTTTTGTCCTTTACTGCT+TGG | - | Chr4:4361697-4361716 | None:intergenic | 45.0% |
| !! | GTAAAAAATATATATAATAA+AGG | - | Chr4:4361201-4361220 | None:intergenic | 5.0% |
| CAAGCAGCTAAGGAGAAGAC+AGG | + | Chr4:4361629-4361648 | MsG0480018398.01.T01:CDS | 50.0% | |
| CAAGGGTTATGAAATGGGCC+AGG | + | Chr4:4361775-4361794 | MsG0480018398.01.T01:CDS | 50.0% | |
| CACATTGCCCATCATCTGGT+TGG | - | Chr4:4361564-4361583 | None:intergenic | 50.0% | |
| CAGGAAAGGACAACTCAGCT+GGG | + | Chr4:4361819-4361838 | MsG0480018398.01.T01:CDS | 50.0% | |
| CTGTCTTCTCCTTAGCTGCT+TGG | - | Chr4:4361631-4361650 | None:intergenic | 50.0% | |
| GCAAACAGCCCAAGCAACAA+AGG | + | Chr4:4361718-4361737 | MsG0480018398.01.T01:CDS | 50.0% | |
| GCAAACAGCCCAAGCAGTAA+AGG | + | Chr4:4361685-4361704 | MsG0480018398.01.T01:CDS | 50.0% | |
| GGCACAAGCTGCAAAGGAAA+AGG | + | Chr4:4361592-4361611 | MsG0480018398.01.T01:CDS | 50.0% | |
| TACAGAGCTGGTGAGACCAA+GGG | + | Chr4:4360914-4360933 | MsG0480018398.01.T01:CDS | 50.0% | |
| TACTGCTTGGGCTGTTTGCT+GGG | - | Chr4:4361684-4361703 | None:intergenic | 50.0% | |
| TATGGCACAAGGTGCAACTG+AGG | + | Chr4:4361874-4361893 | MsG0480018398.01.T01:CDS | 50.0% | |
| TCAGGAAAGGACAACTCAGC+TGG | + | Chr4:4361818-4361837 | MsG0480018398.01.T01:CDS | 50.0% | |
| TGACAAGGCACAAGCTGCAA+AGG | + | Chr4:4361586-4361605 | MsG0480018398.01.T01:CDS | 50.0% | |
| TTACTGCTTGGGCTGTTTGC+TGG | - | Chr4:4361685-4361704 | None:intergenic | 50.0% | |
| ! | GCTGGGTTTTTGCAACAGAC+AGG | + | Chr4:4361836-4361855 | MsG0480018398.01.T01:CDS | 50.0% |
| ! | GGGTTTTCTCCTTTGCAGCT+TGG | - | Chr4:4361664-4361683 | None:intergenic | 50.0% |
| ! | TGTTGCTTGGGCTGTTTGCT+GGG | - | Chr4:4361717-4361736 | None:intergenic | 50.0% |
| ! | TTGTTGCTTGGGCTGTTTGC+TGG | - | Chr4:4361718-4361737 | None:intergenic | 50.0% |
| !! | AGCTCTGTAGCTCTGATCGT+GGG | - | Chr4:4360903-4360922 | None:intergenic | 50.0% |
| !! | CAACCAGATGATGGGCAATG+TGG | + | Chr4:4361562-4361581 | MsG0480018398.01.T01:CDS | 50.0% |
| ACAGACAGCCCAAGCTGCAA+AGG | + | Chr4:4361652-4361671 | MsG0480018398.01.T01:CDS | 55.0% | |
| CACCCACATTGCCCATCATC+TGG | - | Chr4:4361568-4361587 | None:intergenic | 55.0% | |
| CACGATCAGAGCTACAGAGC+TGG | + | Chr4:4360902-4360921 | MsG0480018398.01.T01:CDS | 55.0% | |
| CTACAGAGCTGGTGAGACCA+AGG | + | Chr4:4360913-4360932 | MsG0480018398.01.T01:CDS | 55.0% | |
| GATGGGCAATGTGGGTGACA+AGG | + | Chr4:4361571-4361590 | MsG0480018398.01.T01:CDS | 55.0% | |
| GCAAACAGCCCAAGCAGCTA+AGG | + | Chr4:4361619-4361638 | MsG0480018398.01.T01:CDS | 55.0% | |
| TGAGACCAAGGGTCGAACTG+AGG | + | Chr4:4360925-4360944 | MsG0480018398.01.T01:CDS | 55.0% | |
| ! | GGGCAGTTTCTTTGGTAGCC+TGG | - | Chr4:4361796-4361815 | None:intergenic | 55.0% |
| !! | CAGCTCTGTAGCTCTGATCG+TGG | - | Chr4:4360904-4360923 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 4360893 | 4361997 | 4360893 | ID=MsG0480018398.01;Name=MsG0480018398.01 |
| Chr4 | mRNA | 4360893 | 4361997 | 4360893 | ID=MsG0480018398.01.T01;Parent=MsG0480018398.01;Name=MsG0480018398.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|165 |
| Chr4 | exon | 4360893 | 4360946 | 4360893 | ID=MsG0480018398.01.T01:exon:1176;Parent=MsG0480018398.01.T01 |
| Chr4 | exon | 4361554 | 4361997 | 4361554 | ID=MsG0480018398.01.T01:exon:1177;Parent=MsG0480018398.01.T01 |
| Chr4 | CDS | 4360893 | 4360946 | 4360893 | ID=MsG0480018398.01.T01:cds;Parent=MsG0480018398.01.T01 |
| Chr4 | CDS | 4361554 | 4361997 | 4361554 | ID=MsG0480018398.01.T01:cds;Parent=MsG0480018398.01.T01 |
| Gene Sequence |
| Protein sequence |
