Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018469.01.T01 | AES86647.1 | 95.902 | 122 | 5 | 0 | 1 | 122 | 1 | 122 | 1.07E-70 | 217 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018469.01.T01 | G7JJB1 | 95.902 | 122 | 5 | 0 | 1 | 122 | 1 | 122 | 5.13e-71 | 217 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480018469.01 | MsG0680031628.01 | 0.825589 | 4.316036e-54 | 3.950980e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018469.01.T01 | MTR_4g012510 | 95.902 | 122 | 5 | 0 | 1 | 122 | 1 | 122 | 1.30e-74 | 217 |
| MsG0480018469.01.T01 | MTR_4g012390 | 65.854 | 123 | 39 | 3 | 1 | 122 | 11 | 131 | 9.80e-53 | 164 |
| MsG0480018469.01.T01 | MTR_4g012350 | 72.973 | 111 | 28 | 2 | 1 | 110 | 1 | 110 | 3.51e-51 | 158 |
| MsG0480018469.01.T01 | MTR_4g012490 | 73.984 | 123 | 31 | 1 | 1 | 122 | 1 | 123 | 2.33e-50 | 156 |
| MsG0480018469.01.T01 | MTR_4g012460 | 72.034 | 118 | 31 | 2 | 1 | 116 | 1 | 118 | 9.17e-47 | 147 |
| MsG0480018469.01.T01 | MTR_4g012330 | 62.903 | 124 | 31 | 3 | 1 | 122 | 8 | 118 | 8.77e-43 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGATCTTGAAACATTATC+AGG | 0.352452 | 4:+5368411 | None:intergenic |
| AATTCATTGACACATACAAT+AGG | 0.384648 | 4:+5368585 | None:intergenic |
| GAAGAAATATTTGAGCTAAA+TGG | 0.409187 | 4:-5368327 | MsG0480018469.01.T01:CDS |
| AAATTCTGTAAGTAAGAGTT+AGG | 0.427288 | 4:+5368456 | None:intergenic |
| TAGCAAGCTTGAAATCTTTG+TGG | 0.460073 | 4:-5368545 | MsG0480018469.01.T01:CDS |
| TACTTACAGAATTTCGATGA+TGG | 0.477555 | 4:-5368447 | MsG0480018469.01.T01:CDS |
| ATTCATTGACACATACAATA+GGG | 0.488536 | 4:+5368586 | None:intergenic |
| TGAGAAGAATATTGAAGTGA+TGG | 0.543983 | 4:-5368355 | MsG0480018469.01.T01:CDS |
| TTGTCAAGGTATGAGAGACT+TGG | 0.570815 | 4:-5368615 | MsG0480018469.01.T01:CDS |
| TGAAACATTATCAGGATCAG+TGG | 0.593398 | 4:+5368419 | None:intergenic |
| CAAGCTTGAAATCTTTGTGG+AGG | 0.605933 | 4:-5368542 | MsG0480018469.01.T01:CDS |
| TTAGGATCATATATAAACAC+AGG | 0.655201 | 4:+5368474 | None:intergenic |
| CATACCTTGACAAAATGCTG+TGG | 0.660621 | 4:+5368625 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATTCTTCTCAAAAATCT+TGG | + | Chr4:5368618-5368637 | None:intergenic | 20.0% |
| ! | AAATTCTGTAAGTAAGAGTT+AGG | + | Chr4:5368527-5368546 | None:intergenic | 25.0% |
| ! | AATGATCTTGAAACATTATC+AGG | + | Chr4:5368572-5368591 | None:intergenic | 25.0% |
| ! | AATTCATTGACACATACAAT+AGG | + | Chr4:5368398-5368417 | None:intergenic | 25.0% |
| ! | ATTCATTGACACATACAATA+GGG | + | Chr4:5368397-5368416 | None:intergenic | 25.0% |
| ! | GAAGAAATATTTGAGCTAAA+TGG | - | Chr4:5368653-5368672 | MsG0480018469.01.T01:CDS | 25.0% |
| ! | TTAGGATCATATATAAACAC+AGG | + | Chr4:5368509-5368528 | None:intergenic | 25.0% |
| AGATCAAGAGAGAAAAAAAG+AGG | - | Chr4:5368462-5368481 | MsG0480018469.01.T01:CDS | 30.0% | |
| TACTTACAGAATTTCGATGA+TGG | - | Chr4:5368533-5368552 | MsG0480018469.01.T01:CDS | 30.0% | |
| TGAGAAGAATATTGAAGTGA+TGG | - | Chr4:5368625-5368644 | MsG0480018469.01.T01:CDS | 30.0% | |
| TTCTTCTCAAAAATCTTGGA+CGG | + | Chr4:5368614-5368633 | None:intergenic | 30.0% | |
| TAGCAAGCTTGAAATCTTTG+TGG | - | Chr4:5368435-5368454 | MsG0480018469.01.T01:CDS | 35.0% | |
| TGAAACATTATCAGGATCAG+TGG | + | Chr4:5368564-5368583 | None:intergenic | 35.0% | |
| CAAGCTTGAAATCTTTGTGG+AGG | - | Chr4:5368438-5368457 | MsG0480018469.01.T01:CDS | 40.0% | |
| CATACCTTGACAAAATGCTG+TGG | + | Chr4:5368358-5368377 | None:intergenic | 40.0% | |
| TTGTCAAGGTATGAGAGACT+TGG | - | Chr4:5368365-5368384 | MsG0480018469.01.T01:CDS | 40.0% | |
| ! | GAAACCACAGCATTTTGTCA+AGG | - | Chr4:5368351-5368370 | MsG0480018469.01.T01:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 5368317 | 5368685 | 5368317 | ID=MsG0480018469.01;Name=MsG0480018469.01 |
| Chr4 | mRNA | 5368317 | 5368685 | 5368317 | ID=MsG0480018469.01.T01;Parent=MsG0480018469.01;Name=MsG0480018469.01.T01;_AED=0.19;_eAED=0.19;_QI=0|-1|0|1|-1|1|1|0|122 |
| Chr4 | exon | 5368317 | 5368685 | 5368317 | ID=MsG0480018469.01.T01:exon:626;Parent=MsG0480018469.01.T01 |
| Chr4 | CDS | 5368317 | 5368685 | 5368317 | ID=MsG0480018469.01.T01:cds;Parent=MsG0480018469.01.T01 |
| Gene Sequence |
| Protein sequence |
