AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031628.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031628.01.T01 MTR_7g098290 95.604 91 4 0 40 130 11 101 3.67e-59 180
MsG0680031628.01.T01 MTR_1g069585 95.604 91 4 0 40 130 11 101 3.67e-59 180
MsG0680031628.01.T01 MTR_7g098290 95.349 86 4 0 45 130 1 86 4.06e-55 169
MsG0680031628.01.T01 MTR_1g026090 74.725 91 8 1 40 130 51 126 4.29e-40 133
MsG0680031628.01.T01 MTR_5g464620 85.714 49 7 0 43 91 16 64 1.10e-23 88.2
MsG0680031628.01.T01 MTR_5g054560 54.651 86 15 1 45 130 1 62 2.47e-22 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031628.01.T01 AT3G04400 94.505 91 5 0 40 130 11 101 2.50e-58 178
MsG0680031628.01.T01 AT2G33370 94.505 91 5 0 40 130 11 101 2.50e-58 178
MsG0680031628.01.T01 AT1G04480 94.505 91 5 0 40 130 11 101 2.50e-58 178
MsG0680031628.01.T01 AT3G04400 94.186 86 5 0 45 130 1 86 2.45e-54 167
MsG0680031628.01.T01 AT2G33370 94.186 86 5 0 45 130 1 86 2.45e-54 167

Find 44 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACAAGTTCCGTATGTCAC+TGG 0.413902 6:-23540734 MsG0680031628.01.T01:CDS
ACATGAAGACACCGTCCTTT+CGG 0.413999 6:+23540500 None:intergenic
CCTACCCTTGATTCCCTTCA+CGG 0.419579 6:+23540645 None:intergenic
AAAACATTCTTCCTGAGATC+AGG 0.427356 6:+23540559 None:intergenic
CGTCTTCCTTCAGCTTGTGC+TGG 0.450748 6:-23540616 MsG0680031628.01.T01:CDS
AATCAAATTTGATTGTGTAA+TGG 0.474907 6:-23540211 MsG0680031628.01.T01:three_prime_UTR
GTCGTCATCAAAATCAGATT+CGG 0.481524 6:-23540934 MsG0680031628.01.T01:CDS
CATCTCCGTGAAGGGAATCA+AGG 0.491257 6:-23540650 MsG0680031628.01.T01:CDS
TCATTGTGCTACCTGTCTGC+TGG 0.492839 6:-23540315 MsG0680031628.01.T01:three_prime_UTR
GCATGTACAGTTCAAGTTGA+CGG 0.495697 6:-23540912 MsG0680031628.01.T01:CDS
GGTGTCTTCATGTACTTTGA+AGG 0.499615 6:-23540490 MsG0680031628.01.T01:CDS
CAGTTCACCGTCGCTGCCAC+TGG 0.502403 6:+23540706 None:intergenic
CACATCATGCTGTTTAAGAC+TGG 0.504076 6:+23540348 None:intergenic
TTGTGCTGGTGACGTGGTGA+TGG 0.519017 6:-23540602 MsG0680031628.01.T01:CDS
AGAAGGGAAAGCCTGATCTC+AGG 0.525390 6:-23540570 MsG0680031628.01.T01:CDS
TGCTGACGAACGATGACAGC+TGG 0.538722 6:+23540535 None:intergenic
TTAGGTCGTGGTGGTTCCGC+GGG 0.540678 6:-23540756 MsG0680031628.01.T01:intron
ACACCGTCCTTTCGGCGCCA+TGG 0.546165 6:+23540508 None:intergenic
AACAAGTTCCGTATGTCACT+GGG 0.554811 6:-23540733 MsG0680031628.01.T01:CDS
AAGCCATGGCGCCGAAAGGA+CGG 0.558400 6:-23540511 MsG0680031628.01.T01:CDS
ATTAGGTCGTGGTGGTTCCG+CGG 0.565472 6:-23540757 MsG0680031628.01.T01:intron
AACGAGATTTACCAGCAGAC+AGG 0.573906 6:+23540304 None:intergenic
CTGGTAGACCCAGTGACATA+CGG 0.574663 6:+23540725 None:intergenic
TGAGTGATCTGTGTTCTGAT+TGG 0.576927 6:-23540407 MsG0680031628.01.T01:three_prime_UTR
GGTGATGGCTACTGTGAAGA+AGG 0.577572 6:-23540587 MsG0680031628.01.T01:CDS
TTCAGCTTGTGCTGGTGACG+TGG 0.580455 6:-23540608 MsG0680031628.01.T01:CDS
GGGTCTACCAGTGGCAGCGA+CGG 0.583652 6:-23540713 MsG0680031628.01.T01:CDS
CTGTACATCATCTCCGTGAA+GGG 0.589573 6:-23540658 MsG0680031628.01.T01:CDS
GATGTACAGATTCTTTGCTC+CGG 0.589715 6:+23540672 None:intergenic
ATCTCCGTGAAGGGAATCAA+GGG 0.592804 6:-23540649 MsG0680031628.01.T01:CDS
TCTGTACATCATCTCCGTGA+AGG 0.600106 6:-23540659 MsG0680031628.01.T01:CDS
GCATAAGCCATGGCGCCGAA+AGG 0.608624 6:-23540515 MsG0680031628.01.T01:CDS
ACGTCACCAGCACAAGCTGA+AGG 0.618315 6:+23540610 None:intergenic
AACGCAACCATGTCGAAGAG+AGG 0.636545 6:-23540857 MsG0680031628.01.T01:intron
TCGTTCGTCAGCATAAGCCA+TGG 0.638983 6:-23540525 MsG0680031628.01.T01:CDS
CCGTGAAGGGAATCAAGGGT+AGG 0.651559 6:-23540645 MsG0680031628.01.T01:CDS
TCTTGTTCGATTGAGAGCGG+TGG 0.651829 6:-23540888 MsG0680031628.01.T01:CDS
GGTTCTTGTTCGATTGAGAG+CGG 0.656937 6:-23540891 MsG0680031628.01.T01:CDS
TTCTGATTGGTCATTCACAA+TGG 0.659883 6:-23540394 MsG0680031628.01.T01:three_prime_UTR
TATGTCACTGGGTCTACCAG+TGG 0.667978 6:-23540722 MsG0680031628.01.T01:CDS
GTGATGGCTACTGTGAAGAA+GGG 0.669945 6:-23540586 MsG0680031628.01.T01:CDS
GTACATACCTCTCTTCGACA+TGG 0.681454 6:+23540850 None:intergenic
ACATACGGAACTTGTTCCCG+CGG 0.684378 6:+23540740 None:intergenic
GTGAACTGTGCTGATAACAC+CGG 0.699857 6:-23540691 MsG0680031628.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAAATTTGATTGTGTAA+TGG - Chr6:23540936-23540955 MsG0680031628.01.T01:CDS 20.0%
! AATACAATTGAATCTGAGAA+AGG + Chr6:23540336-23540355 None:intergenic 25.0%
! TGTATCCCTATTTTCTGATT+AGG - Chr6:23540373-23540392 MsG0680031628.01.T01:three_prime_UTR 30.0%
AAAACATTCTTCCTGAGATC+AGG + Chr6:23540591-23540610 None:intergenic 35.0%
CACGACCTAATCAGAAAATA+GGG + Chr6:23540381-23540400 None:intergenic 35.0%
GTCGTCATCAAAATCAGATT+CGG - Chr6:23540213-23540232 MsG0680031628.01.T01:three_prime_UTR 35.0%
! TTCTGATTGGTCATTCACAA+TGG - Chr6:23540753-23540772 MsG0680031628.01.T01:CDS 35.0%
AACAAGTTCCGTATGTCACT+GGG - Chr6:23540414-23540433 MsG0680031628.01.T01:three_prime_UTR 40.0%
CCACGACCTAATCAGAAAAT+AGG + Chr6:23540382-23540401 None:intergenic 40.0%
GATGTACAGATTCTTTGCTC+CGG + Chr6:23540478-23540497 None:intergenic 40.0%
GGTGTCTTCATGTACTTTGA+AGG - Chr6:23540657-23540676 MsG0680031628.01.T01:CDS 40.0%
TGAGTGATCTGTGTTCTGAT+TGG - Chr6:23540740-23540759 MsG0680031628.01.T01:CDS 40.0%
! ATTTTCTGATTAGGTCGTGG+TGG - Chr6:23540382-23540401 MsG0680031628.01.T01:three_prime_UTR 40.0%
! CACATCATGCTGTTTAAGAC+TGG + Chr6:23540802-23540821 None:intergenic 40.0%
! CCTATTTTCTGATTAGGTCG+TGG - Chr6:23540379-23540398 MsG0680031628.01.T01:three_prime_UTR 40.0%
!! GCATGTACAGTTCAAGTTGA+CGG - Chr6:23540235-23540254 MsG0680031628.01.T01:three_prime_UTR 40.0%
AACGAGATTTACCAGCAGAC+AGG + Chr6:23540846-23540865 None:intergenic 45.0%
ACATGAAGACACCGTCCTTT+CGG + Chr6:23540650-23540669 None:intergenic 45.0%
ATCTCCGTGAAGGGAATCAA+GGG - Chr6:23540498-23540517 MsG0680031628.01.T01:CDS 45.0%
CTGTACATCATCTCCGTGAA+GGG - Chr6:23540489-23540508 MsG0680031628.01.T01:CDS 45.0%
GAACAAGTTCCGTATGTCAC+TGG - Chr6:23540413-23540432 MsG0680031628.01.T01:three_prime_UTR 45.0%
GGTTCTTGTTCGATTGAGAG+CGG - Chr6:23540256-23540275 MsG0680031628.01.T01:three_prime_UTR 45.0%
GTACATACCTCTCTTCGACA+TGG + Chr6:23540300-23540319 None:intergenic 45.0%
GTGATGGCTACTGTGAAGAA+GGG - Chr6:23540561-23540580 MsG0680031628.01.T01:CDS 45.0%
TCTGTACATCATCTCCGTGA+AGG - Chr6:23540488-23540507 MsG0680031628.01.T01:CDS 45.0%
! GTGAACTGTGCTGATAACAC+CGG - Chr6:23540456-23540475 MsG0680031628.01.T01:CDS 45.0%
AACGCAACCATGTCGAAGAG+AGG - Chr6:23540290-23540309 MsG0680031628.01.T01:three_prime_UTR 50.0%
AGAAGGGAAAGCCTGATCTC+AGG - Chr6:23540577-23540596 MsG0680031628.01.T01:CDS 50.0%
CATCTCCGTGAAGGGAATCA+AGG - Chr6:23540497-23540516 MsG0680031628.01.T01:CDS 50.0%
CCTACCCTTGATTCCCTTCA+CGG + Chr6:23540505-23540524 None:intergenic 50.0%
CTGGTAGACCCAGTGACATA+CGG + Chr6:23540425-23540444 None:intergenic 50.0%
TATGTCACTGGGTCTACCAG+TGG - Chr6:23540425-23540444 MsG0680031628.01.T01:exon 50.0%
TCATTGTGCTACCTGTCTGC+TGG - Chr6:23540832-23540851 MsG0680031628.01.T01:intron 50.0%
TCGTTCGTCAGCATAAGCCA+TGG - Chr6:23540622-23540641 MsG0680031628.01.T01:CDS 50.0%
TCTTGTTCGATTGAGAGCGG+TGG - Chr6:23540259-23540278 MsG0680031628.01.T01:three_prime_UTR 50.0%
! ACATACGGAACTTGTTCCCG+CGG + Chr6:23540410-23540429 None:intergenic 50.0%
! GGTGATGGCTACTGTGAAGA+AGG - Chr6:23540560-23540579 MsG0680031628.01.T01:CDS 50.0%
ACGTCACCAGCACAAGCTGA+AGG + Chr6:23540540-23540559 None:intergenic 55.0%
ATTAGGTCGTGGTGGTTCCG+CGG - Chr6:23540390-23540409 MsG0680031628.01.T01:three_prime_UTR 55.0%
CCGTGAAGGGAATCAAGGGT+AGG - Chr6:23540502-23540521 MsG0680031628.01.T01:CDS 55.0%
CGTCTTCCTTCAGCTTGTGC+TGG - Chr6:23540531-23540550 MsG0680031628.01.T01:CDS 55.0%
TGCTGACGAACGATGACAGC+TGG + Chr6:23540615-23540634 None:intergenic 55.0%
! TTCAGCTTGTGCTGGTGACG+TGG - Chr6:23540539-23540558 MsG0680031628.01.T01:CDS 55.0%
! TTGTGCTGGTGACGTGGTGA+TGG - Chr6:23540545-23540564 MsG0680031628.01.T01:CDS 55.0%
TTAGGTCGTGGTGGTTCCGC+GGG - Chr6:23540391-23540410 MsG0680031628.01.T01:three_prime_UTR 60.0%
! AAGCCATGGCGCCGAAAGGA+CGG - Chr6:23540636-23540655 MsG0680031628.01.T01:CDS 60.0%
! GCATAAGCCATGGCGCCGAA+AGG - Chr6:23540632-23540651 MsG0680031628.01.T01:CDS 60.0%
ACACCGTCCTTTCGGCGCCA+TGG + Chr6:23540642-23540661 None:intergenic 65.0%
CAGTTCACCGTCGCTGCCAC+TGG + Chr6:23540444-23540463 None:intergenic 65.0%
GGGTCTACCAGTGGCAGCGA+CGG - Chr6:23540434-23540453 MsG0680031628.01.T01:exon 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 23540211 23540958 23540211 ID=MsG0680031628.01;Name=MsG0680031628.01
Chr6 mRNA 23540211 23540958 23540211 ID=MsG0680031628.01.T01;Parent=MsG0680031628.01;Name=MsG0680031628.01.T01;_AED=0.05;_eAED=0.05;_QI=0|1|0.5|1|1|1|2|227|145
Chr6 exon 23540211 23540774 23540211 ID=MsG0680031628.01.T01:exon:14646;Parent=MsG0680031628.01.T01
Chr6 exon 23540858 23540958 23540858 ID=MsG0680031628.01.T01:exon:14645;Parent=MsG0680031628.01.T01
Chr6 CDS 23540858 23540958 23540858 ID=MsG0680031628.01.T01:cds;Parent=MsG0680031628.01.T01
Chr6 CDS 23540438 23540774 23540438 ID=MsG0680031628.01.T01:cds;Parent=MsG0680031628.01.T01
Chr6 three_prime_UTR 23540211 23540437 23540211 ID=MsG0680031628.01.T01:three_prime_utr;Parent=MsG0680031628.01.T01
Gene Sequence

>MsG0680031628.01.T01

ATGTCGTCATCAAAATCAGATTCGGCATGTACAGTTCAAGTTGACGGTTCTTGTTCGATTGAGAGCGGTGGTAACAAAGAACGCAACCATGTCGAAGAGAGGTCGTGGTGGTTCCGCGGGAACAAGTTCCGTATGTCACTGGGTCTACCAGTGGCAGCGACGGTGAACTGTGCTGATAACACCGGAGCAAAGAATCTGTACATCATCTCCGTGAAGGGAATCAAGGGTAGGTTGAATCGTCTTCCTTCAGCTTGTGCTGGTGACGTGGTGATGGCTACTGTGAAGAAGGGAAAGCCTGATCTCAGGAAGAATGTTTTGCCAGCTGTCATCGTTCGTCAGCATAAGCCATGGCGCCGAAAGGACGGTGTCTTCATGTACTTTGAAGGTAACAAACGTTGTTTTGATTCATGTTTTGATTTTGAAATGTTGCTTCTGTAA

Protein sequence

>MsG0680031628.01.T01

MSSSKSDSACTVQVDGSCSIESGGNKERNHVEERSWWFRGNKFRMSLGLPVAATVNCADNTGAKNLYIISVKGIKGRLNRLPSACAGDVVMATVKKGKPDLRKNVLPAVIVRQHKPWRRKDGVFMYFEGNKRCFDSCFDFEMLLL*