Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018652.01.T01 | RHN61350.1 | 87.77 | 139 | 4 | 1 | 1 | 139 | 106 | 231 | 3.22E-77 | 238 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018652.01.T01 | Q8L794 | 64.964 | 137 | 35 | 2 | 1 | 137 | 101 | 224 | 8.73E-54 | 178 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018652.01.T01 | A0A396I6Y1 | 87.770 | 139 | 4 | 1 | 1 | 139 | 106 | 231 | 1.54e-77 | 238 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048909.01 | MsG0480018652.01 | 0.819730 | 9.961609e-53 | 7.728597e-50 |
| MsG0280006451.01 | MsG0480018652.01 | 0.810718 | 1.002721e-50 | 6.089856e-48 |
| MsG0480018651.01 | MsG0480018652.01 | 0.990983 | 2.678507e-185 | 1.957981e-178 |
| MsG0480018652.01 | MsG0480021308.01 | 0.827471 | 1.535877e-54 | 1.484240e-51 |
| MsG0480018652.01 | MsG0480023469.01 | 0.800151 | 1.648980e-48 | 7.622230e-46 |
| MsG0480018652.01 | MsG0780039879.01 | 0.809845 | 1.546751e-50 | 9.180021e-48 |
| MsG0480018652.01 | MsG0780040321.01 | 0.800448 | 1.434555e-48 | 6.681225e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018652.01.T01 | MTR_4g071230 | 84.672 | 137 | 8 | 1 | 1 | 137 | 106 | 229 | 3.62e-75 | 231 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018652.01.T01 | AT2G21370 | 64.964 | 137 | 35 | 2 | 1 | 137 | 8 | 131 | 1.24e-55 | 176 |
| MsG0480018652.01.T01 | AT2G21370 | 64.964 | 137 | 35 | 2 | 1 | 137 | 8 | 131 | 2.97e-55 | 177 |
| MsG0480018652.01.T01 | AT2G21370 | 64.964 | 137 | 35 | 2 | 1 | 137 | 101 | 224 | 3.97e-55 | 178 |
| MsG0480018652.01.T01 | AT2G21370 | 64.964 | 137 | 35 | 2 | 1 | 137 | 101 | 224 | 8.90e-55 | 178 |
Find 22 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGTATTTGCGAAGGTTAAA+TGG | 0.181039 | 4:+8046583 | None:intergenic |
| ATTGTGTCGATTTCTATTAA+TGG | 0.281116 | 4:-8046562 | MsG0480018651.01.T01:intron |
| TGGCTTCTTCATGGAAAGCT+TGG | 0.398484 | 4:-8046034 | MsG0480018651.01.T01:CDS |
| CAACTACTATCATTGTTGAT+AGG | 0.411174 | 4:-8046531 | MsG0480018651.01.T01:intron |
| GCAGAGCATACTGTATGATT+TGG | 0.422912 | 4:+8046151 | None:intergenic |
| TGTATTTGCGAAGGTTAAAT+GGG | 0.444524 | 4:+8046584 | None:intergenic |
| TTTACAGCAGGTAGTGCATC+AGG | 0.446244 | 4:+8046187 | None:intergenic |
| TGTTATTGCACCAAGCAGAT+TGG | 0.463073 | 4:-8046063 | MsG0480018651.01.T01:CDS |
| GCCACAAAAGCCAATCTGCT+TGG | 0.490266 | 4:+8046053 | None:intergenic |
| CAACTCTCATTGTAAAGTAA+AGG | 0.495280 | 4:+8046211 | None:intergenic |
| ATTGTATTTGAATTCTGAAG+TGG | 0.515976 | 4:-8046275 | MsG0480018651.01.T01:intron |
| TTGTATTTGAATTCTGAAGT+GGG | 0.540591 | 4:-8046274 | MsG0480018651.01.T01:intron |
| AGATTATAATAATGCTCTGA+AGG | 0.544841 | 4:-8046005 | MsG0480018651.01.T01:intron |
| CTCTGTGTAAGCTCGTCTCA+TGG | 0.548998 | 4:-8046120 | MsG0480018651.01.T01:CDS |
| TGTATGATTTGGAGGTGCAA+TGG | 0.586369 | 4:+8046162 | None:intergenic |
| TCGACACAATGTATTTGCGA+AGG | 0.590674 | 4:+8046575 | None:intergenic |
| GAGCATACTGTATGATTTGG+AGG | 0.597300 | 4:+8046154 | None:intergenic |
| GTCTCATGGTGGAATCGTGA+CGG | 0.610761 | 4:-8046106 | MsG0480018651.01.T01:CDS |
| GTGACACAGGAGAACCGTTG+TGG | 0.635099 | 4:-8046237 | MsG0480018651.01.T01:CDS |
| TGAGACGAGCTTACACAGAG+TGG | 0.671797 | 4:+8046123 | None:intergenic |
| TAAAGTAAAGGTCTCCACAA+CGG | 0.675201 | 4:+8046223 | None:intergenic |
| TGTGTAAGCTCGTCTCATGG+TGG | 0.679967 | 4:-8046117 | MsG0480018651.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACAAAATATTGTCAAATTG+CGG | - | Chr4:8046206-8046225 | MsG0480018651.01.T01:CDS | 20.0% |
| ! | AGATTATAATAATGCTCTGA+AGG | - | Chr4:8046596-8046615 | MsG0480018651.01.T01:intron | 25.0% |
| !! | ATTGTATTTGAATTCTGAAG+TGG | - | Chr4:8046326-8046345 | MsG0480018651.01.T01:intron | 25.0% |
| !!! | ATTGTGTCGATTTCTATTAA+TGG | - | Chr4:8046039-8046058 | MsG0480018651.01.T01:CDS | 25.0% |
| !!! | TTGTATTTGAATTCTGAAGT+GGG | - | Chr4:8046327-8046346 | MsG0480018651.01.T01:intron | 25.0% |
| ATGTATTTGCGAAGGTTAAA+TGG | + | Chr4:8046021-8046040 | None:intergenic | 30.0% | |
| CAACTACTATCATTGTTGAT+AGG | - | Chr4:8046070-8046089 | MsG0480018651.01.T01:CDS | 30.0% | |
| CAACTCTCATTGTAAAGTAA+AGG | + | Chr4:8046393-8046412 | None:intergenic | 30.0% | |
| CACTATTTAACAACACTGAT+TGG | + | Chr4:8046120-8046139 | None:intergenic | 30.0% | |
| TGTATTTGCGAAGGTTAAAT+GGG | + | Chr4:8046020-8046039 | None:intergenic | 30.0% | |
| ! | CAATCAGTGTTGTTAAATAG+TGG | - | Chr4:8046118-8046137 | MsG0480018651.01.T01:CDS | 30.0% |
| TAAAGTAAAGGTCTCCACAA+CGG | + | Chr4:8046381-8046400 | None:intergenic | 35.0% | |
| ! | GTGTTGTTAAATAGTGGCTA+TGG | - | Chr4:8046124-8046143 | MsG0480018651.01.T01:CDS | 35.0% |
| GCAGAGCATACTGTATGATT+TGG | + | Chr4:8046453-8046472 | None:intergenic | 40.0% | |
| TCGACACAATGTATTTGCGA+AGG | + | Chr4:8046029-8046048 | None:intergenic | 40.0% | |
| TGTTATTGCACCAAGCAGAT+TGG | - | Chr4:8046538-8046557 | MsG0480018651.01.T01:intron | 40.0% | |
| ! | ATTTTGTACTGCAGTGACAC+AGG | - | Chr4:8046351-8046370 | MsG0480018651.01.T01:intron | 40.0% |
| ! | GAGCATACTGTATGATTTGG+AGG | + | Chr4:8046450-8046469 | None:intergenic | 40.0% |
| ! | TGTATGATTTGGAGGTGCAA+TGG | + | Chr4:8046442-8046461 | None:intergenic | 40.0% |
| TGGCTTCTTCATGGAAAGCT+TGG | - | Chr4:8046567-8046586 | MsG0480018651.01.T01:intron | 45.0% | |
| TTTACAGCAGGTAGTGCATC+AGG | + | Chr4:8046417-8046436 | None:intergenic | 45.0% | |
| ! | TGGCTTTTGTGGCTTCTTCA+TGG | - | Chr4:8046558-8046577 | MsG0480018651.01.T01:intron | 45.0% |
| !! | GGTGCAATGGATTTTACAGC+AGG | + | Chr4:8046429-8046448 | None:intergenic | 45.0% |
| !!! | ACCAAGCAGATTGGCTTTTG+TGG | - | Chr4:8046547-8046566 | MsG0480018651.01.T01:intron | 45.0% |
| AGTGCTGCAATCGCAGATCA+TGG | + | Chr4:8046287-8046306 | None:intergenic | 50.0% | |
| CTCTGTGTAAGCTCGTCTCA+TGG | - | Chr4:8046481-8046500 | MsG0480018651.01.T01:intron | 50.0% | |
| GCCACAAAAGCCAATCTGCT+TGG | + | Chr4:8046551-8046570 | None:intergenic | 50.0% | |
| TGAGACGAGCTTACACAGAG+TGG | + | Chr4:8046481-8046500 | None:intergenic | 50.0% | |
| TGTGTAAGCTCGTCTCATGG+TGG | - | Chr4:8046484-8046503 | MsG0480018651.01.T01:intron | 50.0% | |
| ! | GTCTCATGGTGGAATCGTGA+CGG | - | Chr4:8046495-8046514 | MsG0480018651.01.T01:intron | 50.0% |
| GTGACACAGGAGAACCGTTG+TGG | - | Chr4:8046364-8046383 | MsG0480018651.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 8045985 | 8046638 | 8045985 | ID=MsG0480018652.01;Name=MsG0480018652.01 |
| Chr4 | mRNA | 8045985 | 8046638 | 8045985 | ID=MsG0480018652.01.T01;Parent=MsG0480018652.01;Name=MsG0480018652.01.T01;_AED=0.25;_eAED=0.25;_QI=0|0|0|1|0|0|2|0|139 |
| Chr4 | exon | 8046532 | 8046638 | 8046532 | ID=MsG0480018652.01.T01:exon:1983;Parent=MsG0480018652.01.T01 |
| Chr4 | exon | 8045985 | 8046297 | 8045985 | ID=MsG0480018652.01.T01:exon:1982;Parent=MsG0480018652.01.T01 |
| Chr4 | CDS | 8046532 | 8046638 | 8046532 | ID=MsG0480018652.01.T01:cds;Parent=MsG0480018652.01.T01 |
| Chr4 | CDS | 8045985 | 8046297 | 8045985 | ID=MsG0480018652.01.T01:cds;Parent=MsG0480018652.01.T01 |
| Gene Sequence |
| Protein sequence |
