AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019160.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019160.01.T01 MTR_8g099885 79.279 333 35 3 1 306 1 326 0.0 518
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019160.01.T01 AT1G55090 63.830 329 85 2 1 302 1 322 1.67e-147 432

Find 68 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCATCATTTAAAGAAAAT+TGG 0.129442 4:+15233568 None:intergenic
CATGCTGAGCTTGCATTAAA+TGG 0.156645 4:-15233990 MsG0480019160.01.T01:CDS
ACGCCTTTCTGTTCCTCTTA+AGG 0.304612 4:-15233303 MsG0480019160.01.T01:CDS
TGTTCCAGTGTTGTGGTTAA+TGG 0.316625 4:-15233612 MsG0480019160.01.T01:intron
TGTTAGGTTGCAAGCCTTAG+AGG 0.329786 4:-15233422 MsG0480019160.01.T01:intron
GGAATGTTTGAAAGATATAT+TGG 0.334800 4:-15235240 MsG0480019160.01.T01:CDS
GTCTTTGATTTGTTGAAGAT+TGG 0.361625 4:+15235468 None:intergenic
CATCGAGCTTTCTAAGTTGA+TGG 0.366686 4:+15233934 None:intergenic
ATGCTGAGCTTGCATTAAAT+GGG 0.371833 4:-15233989 MsG0480019160.01.T01:CDS
GACGAACGAAATTCTGTTAT+GGG 0.376097 4:+15233801 None:intergenic
AGACGAACGAAATTCTGTTA+TGG 0.378509 4:+15233800 None:intergenic
ATCATTTGCAAGCCACATCT+TGG 0.378781 4:+15235103 None:intergenic
CTCTCAGAACCTTTGATAAT+AGG 0.381396 4:+15235176 None:intergenic
GACAATAAACTAACCTTAAG+AGG 0.386044 4:+15233290 None:intergenic
AGCATCATTTAAAGAAAATT+GGG 0.387395 4:+15233569 None:intergenic
AAGATATATTGGTTGGTGAT+TGG 0.409668 4:-15235229 MsG0480019160.01.T01:CDS
CTTTACAACTTGCTTGCTCT+TGG 0.410098 4:+15233384 None:intergenic
AGCTACTTAAAGATCCTCTA+AGG 0.411025 4:+15233408 None:intergenic
AACTTCGATGGTTTACTGCT+TGG 0.416004 4:-15235067 MsG0480019160.01.T01:CDS
GATGGTGGTCGCCTTTACTA+TGG 0.421906 4:-15233837 MsG0480019160.01.T01:CDS
GTCGTATCATAATAATCTTT+CGG 0.427232 4:+15235126 None:intergenic
TCCTAATGTTTCCAAGGCAT+TGG 0.435328 4:-15235009 MsG0480019160.01.T01:CDS
CAATCTTAACCAATGGGCTA+TGG 0.462534 4:-15235499 MsG0480019160.01.T01:CDS
GATAACATAGTGTGCAGTAT+TGG 0.463634 4:-15235203 MsG0480019160.01.T01:CDS
GGCTTGTTTAGATTTGTAGA+TGG 0.463676 4:+15235444 None:intergenic
TTCTGAGAGATACAATTGTC+AGG 0.465019 4:-15235162 MsG0480019160.01.T01:CDS
GCCAATGCCTTGGAAACATT+AGG 0.470477 4:+15235008 None:intergenic
TTTAAATGATGCTGAGGCTG+TGG 0.476417 4:-15233559 MsG0480019160.01.T01:CDS
TCATTTGCAAGCCACATCTT+GGG 0.476765 4:+15235104 None:intergenic
TAATGGTGATGTTGTTGCCC+AGG 0.493960 4:-15233595 MsG0480019160.01.T01:CDS
TACTTGCAATCTTAACCAAT+GGG 0.505809 4:-15235505 MsG0480019160.01.T01:CDS
AATCACGATGAGGCTCTTGA+AGG 0.512546 4:-15235532 None:intergenic
TTAAAGAAAATTGGGAACCC+TGG 0.521943 4:+15233577 None:intergenic
TAAAGAAAATTGGGAACCCT+GGG 0.523401 4:+15233578 None:intergenic
GTTTACTGCTTGGAAACAAA+GGG 0.526848 4:-15235057 MsG0480019160.01.T01:CDS
AATGGTGATGTTGTTGCCCA+GGG 0.530890 4:-15233594 MsG0480019160.01.T01:CDS
ACTTCCTCCTAATGTTTCCA+AGG 0.533159 4:-15235015 MsG0480019160.01.T01:CDS
TTAACAAAACCATAACCAAA+AGG 0.535794 4:+15234972 None:intergenic
CTACTTGCAATCTTAACCAA+TGG 0.542643 4:-15235506 MsG0480019160.01.T01:CDS
GCAAGCAAGTTGTAAAGTCA+AGG 0.543230 4:-15233378 MsG0480019160.01.T01:CDS
GAATGAATTCCATAGCCCAT+TGG 0.543742 4:+15235490 None:intergenic
ATTGGAATGCCTATTATCAA+AGG 0.545628 4:-15235185 MsG0480019160.01.T01:CDS
GGTTTACTGCTTGGAAACAA+AGG 0.548451 4:-15235058 MsG0480019160.01.T01:CDS
ACCAAGACGGACGACGACGC+CGG 0.556438 4:+15235423 None:intergenic
GAAGTTGAAAATCATCAAGT+TGG 0.562415 4:+15235033 None:intergenic
GTAATTATAGAGAACTTCGA+TGG 0.573265 4:-15235079 MsG0480019160.01.T01:CDS
ATCTACAAATCTAAACAAGC+CGG 0.574227 4:-15235442 MsG0480019160.01.T01:CDS
GGCGACCACCATCACAACCT+TGG 0.577207 4:+15233847 None:intergenic
AGTAATCACCAAGGTTGTGA+TGG 0.582286 4:-15233855 MsG0480019160.01.T01:CDS
TGTTTGAAAGATATATTGGT+TGG 0.589747 4:-15235236 MsG0480019160.01.T01:CDS
AATCACCAAGGTTGTGATGG+TGG 0.592875 4:-15233852 MsG0480019160.01.T01:CDS
AAGATGTGGCTTGCAAATGA+TGG 0.599672 4:-15235101 MsG0480019160.01.T01:CDS
CTATATAGTACATCTTCTGA+AGG 0.600349 4:+15233353 None:intergenic
TAATGCAAGCTCAGCATGAG+GGG 0.602155 4:+15233995 None:intergenic
ACATGGATGTTCCAGTGTTG+TGG 0.607422 4:-15233619 MsG0480019160.01.T01:intron
TTTAATGCAAGCTCAGCATG+AGG 0.609788 4:+15233993 None:intergenic
TTATGATACGACCCAAGATG+TGG 0.618671 4:-15235115 MsG0480019160.01.T01:CDS
TAACCTTAAGAGGAACAGAA+AGG 0.625303 4:+15233300 None:intergenic
TTAATGCAAGCTCAGCATGA+GGG 0.627773 4:+15233994 None:intergenic
ATAGGTGCTACTCATACTCG+TGG 0.632910 4:-15233894 MsG0480019160.01.T01:CDS
TGCTGAGCTTGCATTAAATG+GGG 0.634241 4:-15233988 MsG0480019160.01.T01:CDS
AATGCCTTGGAAACATTAGG+AGG 0.647704 4:+15235011 None:intergenic
GTGGTCGCCTTTACTATGGT+AGG 0.651504 4:-15233833 MsG0480019160.01.T01:CDS
AATGCAAGCTCAGCATGAGG+GGG 0.672892 4:+15233996 None:intergenic
CATTCTTAGAGATATTGCAG+TGG 0.690113 4:-15234048 MsG0480019160.01.T01:intron
GGTGCTACTCATACTCGTGG+AGG 0.717392 4:-15233891 MsG0480019160.01.T01:CDS
TGCTCTGATAGATCTTGACG+TGG 0.727441 4:-15233535 MsG0480019160.01.T01:intron
TATATGTACAGTAATCACCA+AGG 0.733722 4:-15233864 MsG0480019160.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTTTCTATTATTATTGTT+TGG - Chr4:15234475-15234494 MsG0480019160.01.T01:intron 15.0%
!! ACAAAAATAAAGTGTGAATT+TGG + Chr4:15233881-15233900 None:intergenic 20.0%
!! AGCATCATTTAAAGAAAATT+GGG + Chr4:15235242-15235261 None:intergenic 20.0%
!! ATAAACATATGAGTATAAGT+TGG + Chr4:15234711-15234730 None:intergenic 20.0%
!! GGAAAATAAATATCCTTATA+CGG + Chr4:15235097-15235116 None:intergenic 20.0%
!! TACAAACAATAATTATGTCT+TGG - Chr4:15234147-15234166 MsG0480019160.01.T01:intron 20.0%
!! TGACATTTAAACAATCTAAA+TGG + Chr4:15235118-15235137 None:intergenic 20.0%
!! TGTAAATGCTTTAATGTTAA+TGG + Chr4:15235348-15235367 None:intergenic 20.0%
!! TGTTTAAATGTCAATTATGT+GGG - Chr4:15235125-15235144 MsG0480019160.01.T01:CDS 20.0%
!! TTGTTTAAATGTCAATTATG+TGG - Chr4:15235124-15235143 MsG0480019160.01.T01:CDS 20.0%
!! TTTCTATTATTATTGTTTGG+CGG - Chr4:15234478-15234497 MsG0480019160.01.T01:intron 20.0%
!!! ATGTGTTTCATTTATGTTTT+TGG - Chr4:15234679-15234698 MsG0480019160.01.T01:intron 20.0%
!!! TGTGTTTCATTTATGTTTTT+GGG - Chr4:15234680-15234699 MsG0480019160.01.T01:intron 20.0%
!!! TTTTTCAAGAACATATGTTA+AGG - Chr4:15234120-15234139 MsG0480019160.01.T01:intron 20.0%
! AAAGAAAGAGTTATTACGAA+TGG + Chr4:15233473-15233492 None:intergenic 25.0%
! CAGCATCATTTAAAGAAAAT+TGG + Chr4:15235243-15235262 None:intergenic 25.0%
! GACGTAACATTAATATTTGA+TGG + Chr4:15234437-15234456 None:intergenic 25.0%
! GGAATGTTTGAAAGATATAT+TGG - Chr4:15233568-15233587 MsG0480019160.01.T01:CDS 25.0%
! GTCGTATCATAATAATCTTT+CGG + Chr4:15233685-15233704 None:intergenic 25.0%
! TGACATAAGGTAAAAAGAAA+TGG - Chr4:15234342-15234361 MsG0480019160.01.T01:intron 25.0%
! TGTTTGAAAGATATATTGGT+TGG - Chr4:15233572-15233591 MsG0480019160.01.T01:CDS 25.0%
! TTAACAAAACCATAACCAAA+AGG + Chr4:15233839-15233858 None:intergenic 25.0%
! TTAATATCCATTGTGTGTTT+GGG - Chr4:15235041-15235060 MsG0480019160.01.T01:CDS 25.0%
! TTGATTTCTCAAATCATTAC+TGG + Chr4:15233978-15233997 None:intergenic 25.0%
! TTTAATATCCATTGTGTGTT+TGG - Chr4:15235040-15235059 MsG0480019160.01.T01:CDS 25.0%
!! ATATTTTCCTACCATAGTAA+AGG + Chr4:15234985-15235004 None:intergenic 25.0%
!! ATTTTCTTTAAATGATGCTG+AGG - Chr4:15235243-15235262 MsG0480019160.01.T01:CDS 25.0%
!!! ATTGTGTCATATTTTAACCT+AGG + Chr4:15234283-15234302 None:intergenic 25.0%
!!! GTGTTTCATTTATGTTTTTG+GGG - Chr4:15234681-15234700 MsG0480019160.01.T01:intron 25.0%
!!! TTGTGTCATATTTTAACCTA+GGG + Chr4:15234282-15234301 None:intergenic 25.0%
!!! TTTGTTTTTGTCACTGAAAT+TGG - Chr4:15233528-15233547 MsG0480019160.01.T01:intron 25.0%
ATAATAGAAAAAGAGAGCCA+AGG + Chr4:15234468-15234487 None:intergenic 30.0%
ATAGTTTGAACACACTACTT+AGG + Chr4:15234650-15234669 None:intergenic 30.0%
ATATTTGATGGAGAACAGAA+AGG + Chr4:15234425-15234444 None:intergenic 30.0%
ATCTACAAATCTAAACAAGC+CGG - Chr4:15233366-15233385 MsG0480019160.01.T01:CDS 30.0%
ATTGGAATGCCTATTATCAA+AGG - Chr4:15233623-15233642 MsG0480019160.01.T01:intron 30.0%
CTATATAGTACATCTTCTGA+AGG + Chr4:15235458-15235477 None:intergenic 30.0%
GAAGTTGAAAATCATCAAGT+TGG + Chr4:15233778-15233797 None:intergenic 30.0%
GACAATAAACTAACCTTAAG+AGG + Chr4:15235521-15235540 None:intergenic 30.0%
GAGTTATTACGAATGGTTAA+TGG + Chr4:15233466-15233485 None:intergenic 30.0%
GCATTGCAAGCATTTAAATA+TGG + Chr4:15235312-15235331 None:intergenic 30.0%
GTAATTATAGAGAACTTCGA+TGG - Chr4:15233729-15233748 MsG0480019160.01.T01:intron 30.0%
TACTTGCAATCTTAACCAAT+GGG - Chr4:15233303-15233322 MsG0480019160.01.T01:CDS 30.0%
TATATGTACAGTAATCACCA+AGG - Chr4:15234944-15234963 MsG0480019160.01.T01:intron 30.0%
TTCATCTCATTTGTAACACA+TGG - Chr4:15235172-15235191 MsG0480019160.01.T01:CDS 30.0%
! ACTGCAATATCTCTAAGAAT+GGG + Chr4:15234761-15234780 None:intergenic 30.0%
! AGATTTGTTCCTTTACTGTT+AGG - Chr4:15235370-15235389 MsG0480019160.01.T01:intron 30.0%
! GAAGTTTTTATGAATGCAAG+CGG - Chr4:15234845-15234864 MsG0480019160.01.T01:intron 30.0%
!! AAATCAGTTCCTTTTGGTTA+TGG - Chr4:15233827-15233846 MsG0480019160.01.T01:CDS 30.0%
!! AAGATATATTGGTTGGTGAT+TGG - Chr4:15233579-15233598 MsG0480019160.01.T01:CDS 30.0%
!! GTCTTTGATTTGTTGAAGAT+TGG + Chr4:15233343-15233362 None:intergenic 30.0%
!! TAGTATTTGGTTTGATGCAT+AGG - Chr4:15234079-15234098 MsG0480019160.01.T01:intron 30.0%
!!! GTATTTTGTTTTGCTATCTC+TGG + Chr4:15234388-15234407 None:intergenic 30.0%
ACATTCAGGAAATCCGTATA+AGG - Chr4:15235081-15235100 MsG0480019160.01.T01:CDS 35.0%
AGACGAACGAAATTCTGTTA+TGG + Chr4:15235011-15235030 None:intergenic 35.0%
AGCTACTTAAAGATCCTCTA+AGG + Chr4:15235403-15235422 None:intergenic 35.0%
ATGCTGAGCTTGCATTAAAT+GGG - Chr4:15234819-15234838 MsG0480019160.01.T01:intron 35.0%
CATTCTTAGAGATATTGCAG+TGG - Chr4:15234760-15234779 MsG0480019160.01.T01:intron 35.0%
CGTCATGTTATACTTTCAAC+AGG + Chr4:15234322-15234341 None:intergenic 35.0%
CTACTTGCAATCTTAACCAA+TGG - Chr4:15233302-15233321 MsG0480019160.01.T01:CDS 35.0%
GACGAACGAAATTCTGTTAT+GGG + Chr4:15235010-15235029 None:intergenic 35.0%
GATAACATAGTGTGCAGTAT+TGG - Chr4:15233605-15233624 MsG0480019160.01.T01:CDS 35.0%
GGTAAAAAGAAATGGTACAC+TGG - Chr4:15234350-15234369 MsG0480019160.01.T01:intron 35.0%
GGTTTGATGCATAGGTTAAA+GGG - Chr4:15234087-15234106 MsG0480019160.01.T01:intron 35.0%
GTATAACATGACGTGACATA+AGG - Chr4:15234329-15234348 MsG0480019160.01.T01:intron 35.0%
GTTTACTGCTTGGAAACAAA+GGG - Chr4:15233751-15233770 MsG0480019160.01.T01:intron 35.0%
TAAAGAAAATTGGGAACCCT+GGG + Chr4:15235233-15235252 None:intergenic 35.0%
TAACCTTAAGAGGAACAGAA+AGG + Chr4:15235511-15235530 None:intergenic 35.0%
TCAATCACAAGATTGTTGCA+TGG + Chr4:15234603-15234622 None:intergenic 35.0%
TGGTTTGATGCATAGGTTAA+AGG - Chr4:15234086-15234105 MsG0480019160.01.T01:intron 35.0%
TTAAAGAAAATTGGGAACCC+TGG + Chr4:15235234-15235253 None:intergenic 35.0%
TTATTATTGTTTGGCGGTGT+CGG - Chr4:15234484-15234503 MsG0480019160.01.T01:intron 35.0%
! CACTGCAATATCTCTAAGAA+TGG + Chr4:15234762-15234781 None:intergenic 35.0%
! CTCTCAGAACCTTTGATAAT+AGG + Chr4:15233635-15233654 None:intergenic 35.0%
! GCACAAAAATCAGTTCCTTT+TGG - Chr4:15233821-15233840 MsG0480019160.01.T01:CDS 35.0%
! GGCTTGTTTAGATTTGTAGA+TGG + Chr4:15233367-15233386 None:intergenic 35.0%
! TTCTGAGAGATACAATTGTC+AGG - Chr4:15233646-15233665 MsG0480019160.01.T01:intron 35.0%
!! AGGCGTATTTTAAGGACAAA+AGG + Chr4:15235491-15235510 None:intergenic 35.0%
!! GAACAGAAAGGCGTATTTTA+AGG + Chr4:15235499-15235518 None:intergenic 35.0%
!! TGTTGGTGCTATGTAGTATT+TGG - Chr4:15234066-15234085 MsG0480019160.01.T01:intron 35.0%
AAAGAGAGCCAAGGAAAGTA+AGG + Chr4:15234459-15234478 None:intergenic 40.0%
AACTTCGATGGTTTACTGCT+TGG - Chr4:15233741-15233760 MsG0480019160.01.T01:intron 40.0%
AATGCCTTGGAAACATTAGG+AGG + Chr4:15233800-15233819 None:intergenic 40.0%
ACTTCCTCCTAATGTTTCCA+AGG - Chr4:15233793-15233812 MsG0480019160.01.T01:intron 40.0%
AGTAATCACCAAGGTTGTGA+TGG - Chr4:15234953-15234972 MsG0480019160.01.T01:intron 40.0%
ATATCCATTGTGTGTTTGGG+AGG - Chr4:15235044-15235063 MsG0480019160.01.T01:CDS 40.0%
ATCACCATTAACCACAACAC+TGG + Chr4:15235203-15235222 None:intergenic 40.0%
ATCATTTGCAAGCCACATCT+TGG + Chr4:15233708-15233727 None:intergenic 40.0%
CAACATTTCCGTTGAAGGTA+AGG + Chr4:15234051-15234070 None:intergenic 40.0%
CATGCTGAGCTTGCATTAAA+TGG - Chr4:15234818-15234837 MsG0480019160.01.T01:intron 40.0%
CTTACCTTCAACGGAAATGT+TGG - Chr4:15234049-15234068 MsG0480019160.01.T01:intron 40.0%
GAATGAATTCCATAGCCCAT+TGG + Chr4:15233321-15233340 None:intergenic 40.0%
GACACTTACCTTACCTTCAA+CGG - Chr4:15234040-15234059 MsG0480019160.01.T01:CDS 40.0%
GCAAGCAAGTTGTAAAGTCA+AGG - Chr4:15235430-15235449 MsG0480019160.01.T01:intron 40.0%
GCGACGTAAATTCAACATTC+AGG - Chr4:15235067-15235086 MsG0480019160.01.T01:CDS 40.0%
GGTTTACTGCTTGGAAACAA+AGG - Chr4:15233750-15233769 MsG0480019160.01.T01:intron 40.0%
GTACATGTTAGCATTTCCCT+AGG - Chr4:15234263-15234282 MsG0480019160.01.T01:intron 40.0%
TATCCATTGTGTGTTTGGGA+GGG - Chr4:15235045-15235064 MsG0480019160.01.T01:CDS 40.0%
TCATTTGCAAGCCACATCTT+GGG + Chr4:15233707-15233726 None:intergenic 40.0%
TCCTAATGTTTCCAAGGCAT+TGG - Chr4:15233799-15233818 MsG0480019160.01.T01:intron 40.0%
TGCTGAGCTTGCATTAAATG+GGG - Chr4:15234820-15234839 MsG0480019160.01.T01:intron 40.0%
TGTTACGTCCTTACTTTCCT+TGG - Chr4:15234448-15234467 MsG0480019160.01.T01:intron 40.0%
TGTTCCAGTGTTGTGGTTAA+TGG - Chr4:15235196-15235215 MsG0480019160.01.T01:CDS 40.0%
TTAATGCAAGCTCAGCATGA+GGG + Chr4:15234817-15234836 None:intergenic 40.0%
TTAGAATGCATGAGCGTAGA+AGG + Chr4:15234557-15234576 None:intergenic 40.0%
TTATGATACGACCCAAGATG+TGG - Chr4:15233693-15233712 MsG0480019160.01.T01:intron 40.0%
TTTAAATGATGCTGAGGCTG+TGG - Chr4:15235249-15235268 MsG0480019160.01.T01:intron 40.0%
TTTAATGCAAGCTCAGCATG+AGG + Chr4:15234818-15234837 None:intergenic 40.0%
! AAGATGTGGCTTGCAAATGA+TGG - Chr4:15233707-15233726 MsG0480019160.01.T01:intron 40.0%
! AATCACGTGTCACTGTCATT+TGG - Chr4:15234012-15234031 MsG0480019160.01.T01:CDS 40.0%
! AGAAATGGTACACTGGTTGT+AGG - Chr4:15234357-15234376 MsG0480019160.01.T01:intron 40.0%
! CATCGAGCTTTCTAAGTTGA+TGG + Chr4:15234877-15234896 None:intergenic 40.0%
! CTTTACAACTTGCTTGCTCT+TGG + Chr4:15235427-15235446 None:intergenic 40.0%
! GTTCGTCTTCGTGCTTTTAT+AGG - Chr4:15234896-15234915 MsG0480019160.01.T01:intron 40.0%
! TGATTTTTGTGCCAATGCCT+TGG + Chr4:15233813-15233832 None:intergenic 40.0%
!! ATAACTGGGGATTTCGAGTT+CGG + Chr4:15233412-15233431 None:intergenic 40.0%
!! CAATCTTAACCAATGGGCTA+TGG - Chr4:15233309-15233328 MsG0480019160.01.T01:CDS 40.0%
!!! ATTGGTTTTGTAGCTGCAGA+TGG - Chr4:15233546-15233565 MsG0480019160.01.T01:CDS 40.0%
!!! TTGGTTTTGTAGCTGCAGAT+GGG - Chr4:15233547-15233566 MsG0480019160.01.T01:CDS 40.0%
AATCACCAAGGTTGTGATGG+TGG - Chr4:15234956-15234975 MsG0480019160.01.T01:intron 45.0%
ACATGGATGTTCCAGTGTTG+TGG - Chr4:15235189-15235208 MsG0480019160.01.T01:CDS 45.0%
ACGCCTTTCTGTTCCTCTTA+AGG - Chr4:15235505-15235524 MsG0480019160.01.T01:CDS 45.0%
ACTTACACTGACACGAACAC+GGG - Chr4:15233930-15233949 MsG0480019160.01.T01:CDS 45.0%
AGCACCAACATTTCCGTTGA+AGG + Chr4:15234056-15234075 None:intergenic 45.0%
CACTTACACTGACACGAACA+CGG - Chr4:15233929-15233948 MsG0480019160.01.T01:CDS 45.0%
GAACTCGAAATCCCCAGTTA+TGG - Chr4:15233411-15233430 MsG0480019160.01.T01:CDS 45.0%
GCCAATGCCTTGGAAACATT+AGG + Chr4:15233803-15233822 None:intergenic 45.0%
GGCTTGCAACCTAACAGTAA+AGG + Chr4:15235382-15235401 None:intergenic 45.0%
TAATGCAAGCTCAGCATGAG+GGG + Chr4:15234816-15234835 None:intergenic 45.0%
TGCTCTGATAGATCTTGACG+TGG - Chr4:15235273-15235292 MsG0480019160.01.T01:intron 45.0%
TGGTCTTCACAGCCATAACT+GGG + Chr4:15233426-15233445 None:intergenic 45.0%
! AATGGTGATGTTGTTGCCCA+GGG - Chr4:15235214-15235233 MsG0480019160.01.T01:CDS 45.0%
! GGAGAACAGAAAGGTACAAC+AGG + Chr4:15234416-15234435 None:intergenic 45.0%
! GGTTAATGGTGTCGAGTTCT+AGG + Chr4:15233452-15233471 None:intergenic 45.0%
! TAATGGTGATGTTGTTGCCC+AGG - Chr4:15235213-15235232 MsG0480019160.01.T01:CDS 45.0%
! TGTTAGGTTGCAAGCCTTAG+AGG - Chr4:15235386-15235405 MsG0480019160.01.T01:intron 45.0%
!! ATAGGTGCTACTCATACTCG+TGG - Chr4:15234914-15234933 MsG0480019160.01.T01:intron 45.0%
!! TAACTGGGGATTTCGAGTTC+GGG + Chr4:15233411-15233430 None:intergenic 45.0%
AATGCAAGCTCAGCATGAGG+GGG + Chr4:15234815-15234834 None:intergenic 50.0%
GGTCTTCACAGCCATAACTG+GGG + Chr4:15233425-15233444 None:intergenic 50.0%
GTGGTCGCCTTTACTATGGT+AGG - Chr4:15234975-15234994 MsG0480019160.01.T01:CDS 50.0%
GTGGTCTTCACAGCCATAAC+TGG + Chr4:15233427-15233446 None:intergenic 50.0%
! GATGGTGGTCGCCTTTACTA+TGG - Chr4:15234971-15234990 MsG0480019160.01.T01:CDS 50.0%
! GTGTCGGCGATGTGTTTACT+CGG - Chr4:15234500-15234519 MsG0480019160.01.T01:intron 50.0%
!! TGGTGTCGAGTTCTAGGAAG+TGG + Chr4:15233446-15233465 None:intergenic 50.0%
!! TTTCGAGTTCGGGACCAAGA+CGG + Chr4:15233401-15233420 None:intergenic 50.0%
TCGCCCTCCCAAACACACAA+TGG + Chr4:15235051-15235070 None:intergenic 55.0%
!! GGTGCTACTCATACTCGTGG+AGG - Chr4:15234917-15234936 MsG0480019160.01.T01:intron 55.0%
AGCTCAGCATGAGGGGGAGA+TGG + Chr4:15234809-15234828 None:intergenic 60.0%
GGCGACCACCATCACAACCT+TGG + Chr4:15234964-15234983 None:intergenic 60.0%
ACCAAGACGGACGACGACGC+CGG + Chr4:15233388-15233407 None:intergenic 65.0%
GCCGGCGTCGTCGTCCGTCT+TGG - Chr4:15233384-15233403 MsG0480019160.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 15233283 15235547 15233283 ID=MsG0480019160.01;Name=MsG0480019160.01
Chr4 mRNA 15233283 15235547 15233283 ID=MsG0480019160.01.T01;Parent=MsG0480019160.01;Name=MsG0480019160.01.T01;_AED=0.49;_eAED=0.55;_QI=0|0|0|1|0.75|0.8|5|0|307
Chr4 exon 15235435 15235547 15235435 ID=MsG0480019160.01.T01:exon:4940;Parent=MsG0480019160.01.T01
Chr4 exon 15234960 15235265 15234960 ID=MsG0480019160.01.T01:exon:4939;Parent=MsG0480019160.01.T01
Chr4 exon 15233804 15234061 15233804 ID=MsG0480019160.01.T01:exon:4938;Parent=MsG0480019160.01.T01
Chr4 exon 15233536 15233626 15233536 ID=MsG0480019160.01.T01:exon:4937;Parent=MsG0480019160.01.T01
Chr4 exon 15233283 15233438 15233283 ID=MsG0480019160.01.T01:exon:4936;Parent=MsG0480019160.01.T01
Chr4 CDS 15235435 15235547 15235435 ID=MsG0480019160.01.T01:cds;Parent=MsG0480019160.01.T01
Chr4 CDS 15234960 15235265 15234960 ID=MsG0480019160.01.T01:cds;Parent=MsG0480019160.01.T01
Chr4 CDS 15233804 15234061 15233804 ID=MsG0480019160.01.T01:cds;Parent=MsG0480019160.01.T01
Chr4 CDS 15233536 15233626 15233536 ID=MsG0480019160.01.T01:cds;Parent=MsG0480019160.01.T01
Chr4 CDS 15233283 15233438 15233283 ID=MsG0480019160.01.T01:cds;Parent=MsG0480019160.01.T01
Gene Sequence

>MsG0480019160.01.T01

ATGAGGCTCTTGAAGGTAGCTACTTGCAATCTTAACCAATGGGCTATGGAATTCATTCCAATCTTCAACAAATCAAAGACTCCATCTACAAATCTAAACAAGCCGGCGTCGTCATGGGAATGTTTGAAAGATATATTGGTTGGTGATTGGACTGATAACATAGTGTGCAGTATTGGAATGCCTATTATCAAAGGTTCTGAGAGATACAATTGTCAGGTTTTGTGTTTTAACCGAAAGATTATTATGATACGACCCAAGATGTGGCTTGCAAATGATGGTAATTATAGAGAACTTCGATGGTTTACTGCTTGGAAACAAAGGGACCAACTTGATGATTTTCAACTTCCTCCTAATGTTTCCAAGGCATTGGCACAAAAATCAGTTCCTTTTGGTTATGGTTTTGTTAAGTTTCAAGACACAGATATTGCAGTGGAAGTTTGTGAAAAGCTTTTTACTCCATCTCCCCCTCATGCTGAGCTTGCATTAAATGGGGTTGAAGTTTTTATGAATGCAAGCGGAAGTCACCATCAACTTAGAAAGCTCGATGTTCGTCTTCGTGCTTTTATAGGTGCTACTCATACTCGTGGAGGTGTCTATATGTACAGTAATCACCAAGGTTGTGATGGTGGTCGCCTTTACTATGGTAGGAAAATATTTCCCATAACAGAATTTCGTTCTGTTGTGGTTAATGGTGATGTTGTTGCCCAGGGTTCCCAATTTTCTTTAAATGATGCTGAGGCTGTGGTTGCTCTGATAGATCTTGACGTGGTTGCAAGCCTTAGAGGATCTTTAAGTAGCTTCCAAGAGCAAGCAAGTTGTAAAGTCAAGGTTCCTTCAGAAGATGTACTATATAGTCTTTGTCTTCCTTTTGTCCTTAAAATACGCCTTTCTGTTCCTCTTAAGGTTAGTTTATTGTCATTTTGA
Protein sequence

>MsG0480019160.01.T01

MRLLKVATCNLNQWAMEFIPIFNKSKTPSTNLNKPASSWECLKDILVGDWTDNIVCSIGMPIIKGSERYNCQVLCFNRKIIMIRPKMWLANDGNYRELRWFTAWKQRDQLDDFQLPPNVSKALAQKSVPFGYGFVKFQDTDIAVEVCEKLFTPSPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIGATHTRGGVYMYSNHQGCDGGRLYYGRKIFPITEFRSVVVNGDVVAQGSQFSLNDAEAVVALIDLDVVASLRGSLSSFQEQASCKVKVPSEDVLYSLCLPFVLKIRLSVPLKVSLLSF*