AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019163.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019163.01.T01 MTR_8g099885 96.407 167 6 0 1 167 1 167 1.22e-113 338
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019163.01.T01 AT1G55090 76.647 167 39 0 1 167 1 167 1.38e-94 289

Find 45 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGACGGCGCCGGCTTGTT+TGG 0.281693 4:+15239615 None:intergenic
GTCTTTGATTTGTAGAAGAT+TGG 0.320727 4:+15239651 None:intergenic
GGAATGTTTGAAAGATATAT+TGG 0.334800 4:-15239429 MsG0480019163.01.T01:CDS
TTATTATTACGAATGGTTAA+TGG 0.342917 4:+15239528 None:intergenic
GGCGCCGGCTTGTTTGGATT+TGG 0.343154 4:+15239621 None:intergenic
TCCTAATGTTTCCGAGGCAT+TGG 0.369944 4:-15239198 MsG0480019163.01.T01:CDS
CTCTCAGAACCTTTGATAAT+AGG 0.381396 4:+15239365 None:intergenic
GTGGTCTTCACAGCCATAAC+CGG 0.383872 4:+15239567 None:intergenic
ATAACCGGGGATTTCGAGTT+CGG 0.402614 4:+15239582 None:intergenic
AAGATATATTGGTTGGTGAT+TGG 0.409668 4:-15239418 MsG0480019163.01.T01:CDS
AACTTCGATGGTTTACTGCT+TGG 0.416004 4:-15239256 MsG0480019163.01.T01:CDS
GTCGTATCATAATAATCTTT+CGG 0.425212 4:+15239315 None:intergenic
ATCATTTGCAAGGCACGTCT+TGG 0.435018 4:+15239292 None:intergenic
GAACTCGAAATCCCCGGTTA+TGG 0.442277 4:-15239580 MsG0480019163.01.T01:CDS
CCCAATGCCTCGGAAACATT+AGG 0.451735 4:+15239197 None:intergenic
CCTAATGTTTCCGAGGCATT+GGG 0.451754 4:-15239197 MsG0480019163.01.T01:CDS
TAACCGGGGATTTCGAGTTC+GGG 0.461618 4:+15239583 None:intergenic
CAATCTTAACCAATGGGCTA+TGG 0.462534 4:-15239683 MsG0480019163.01.T01:CDS
GATAACATAGTGTGCAGTAT+TGG 0.463634 4:-15239392 MsG0480019163.01.T01:CDS
TTCTGAGAGATACAATTGTC+AGG 0.465019 4:-15239351 MsG0480019163.01.T01:CDS
TCATTTGCAAGGCACGTCTT+GGG 0.484119 4:+15239293 None:intergenic
GGCTTGTTTGGATTTGGAGA+TGG 0.485111 4:+15239627 None:intergenic
TATAATTATCATCATTTGCA+AGG 0.504657 4:+15239282 None:intergenic
TACTTGCAATCTTAACCAAT+GGG 0.505809 4:-15239689 MsG0480019163.01.T01:CDS
GCCGGCGCCGTCATCCGTCT+TGG 0.507687 4:-15239607 MsG0480019163.01.T01:CDS
AATCACGATGAGGCTCTTGA+AGG 0.512546 4:-15239716 None:intergenic
GAAGTTGAAAATCATAAAGT+TGG 0.522375 4:+15239222 None:intergenic
GTTTACTGCTTGGAAACAAA+GGG 0.526848 4:-15239246 MsG0480019163.01.T01:CDS
ACCAAGACGGATGACGGCGC+CGG 0.530775 4:+15239606 None:intergenic
TGGTCTTCACAGCCATAACC+GGG 0.535392 4:+15239568 None:intergenic
TTAACAAAACCATAACCAAA+AGG 0.535794 4:+15239161 None:intergenic
CTACTTGCAATCTTAACCAA+TGG 0.542643 4:-15239690 MsG0480019163.01.T01:CDS
ATTGGAATGCCTATTATCAA+AGG 0.545628 4:-15239374 MsG0480019163.01.T01:CDS
AATCGAATTCCATAGCCCAT+TGG 0.548373 4:+15239674 None:intergenic
GGTTTACTGCTTGGAAACAA+AGG 0.548451 4:-15239247 MsG0480019163.01.T01:CDS
ATCTCCAAATCCAAACAAGC+CGG 0.565116 4:-15239625 MsG0480019163.01.T01:CDS
ATAATTATAGAGAACTTCGA+TGG 0.573265 4:-15239268 MsG0480019163.01.T01:CDS
TTCGGGACCAAGACGGATGA+CGG 0.577192 4:+15239600 None:intergenic
TGTTTGAAAGATATATTGGT+TGG 0.589747 4:-15239425 MsG0480019163.01.T01:CDS
TTTCGAGTTCGGGACCAAGA+CGG 0.601203 4:+15239593 None:intergenic
ACTTCCTCCTAATGTTTCCG+AGG 0.602942 4:-15239204 MsG0480019163.01.T01:CDS
TGGTGTCGAGTTCTAAGAAG+TGG 0.603974 4:+15239548 None:intergenic
GGTCCCGAACTCGAAATCCC+CGG 0.647848 4:-15239586 MsG0480019163.01.T01:CDS
AATGCCTCGGAAACATTAGG+AGG 0.652440 4:+15239200 None:intergenic
GGTCTTCACAGCCATAACCG+GGG 0.753301 4:+15239569 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATTATCATCATTTGCA+AGG + Chr4:15239575-15239594 None:intergenic 20.0%
!! TTATTATTACGAATGGTTAA+TGG + Chr4:15239329-15239348 None:intergenic 20.0%
!!! GACTGTTTTTCTATTTTAAT+TGG - Chr4:15239382-15239401 MsG0480019163.01.T01:CDS 20.0%
! ATAATTATAGAGAACTTCGA+TGG - Chr4:15239586-15239605 MsG0480019163.01.T01:CDS 25.0%
! GAAAGAGTTATTATTACGAA+TGG + Chr4:15239336-15239355 None:intergenic 25.0%
! GAAGTTGAAAATCATAAAGT+TGG + Chr4:15239635-15239654 None:intergenic 25.0%
! GGAATGTTTGAAAGATATAT+TGG - Chr4:15239425-15239444 MsG0480019163.01.T01:CDS 25.0%
! GTCGTATCATAATAATCTTT+CGG + Chr4:15239542-15239561 None:intergenic 25.0%
! TGTTTGAAAGATATATTGGT+TGG - Chr4:15239429-15239448 MsG0480019163.01.T01:CDS 25.0%
! TTAACAAAACCATAACCAAA+AGG + Chr4:15239696-15239715 None:intergenic 25.0%
ATTGGAATGCCTATTATCAA+AGG - Chr4:15239480-15239499 MsG0480019163.01.T01:intron 30.0%
TACTTGCAATCTTAACCAAT+GGG - Chr4:15239165-15239184 MsG0480019163.01.T01:CDS 30.0%
!! AAATCAGTTCCTTTTGGTTA+TGG - Chr4:15239684-15239703 MsG0480019163.01.T01:CDS 30.0%
!! AAGATATATTGGTTGGTGAT+TGG - Chr4:15239436-15239455 MsG0480019163.01.T01:intron 30.0%
!! GTCTTTGATTTGTAGAAGAT+TGG + Chr4:15239206-15239225 None:intergenic 30.0%
CTACTTGCAATCTTAACCAA+TGG - Chr4:15239164-15239183 MsG0480019163.01.T01:CDS 35.0%
GATAACATAGTGTGCAGTAT+TGG - Chr4:15239462-15239481 MsG0480019163.01.T01:intron 35.0%
GTTTACTGCTTGGAAACAAA+GGG - Chr4:15239608-15239627 MsG0480019163.01.T01:CDS 35.0%
! CTCTCAGAACCTTTGATAAT+AGG + Chr4:15239492-15239511 None:intergenic 35.0%
! GGACAAAAATCAGTTCCTTT+TGG - Chr4:15239678-15239697 MsG0480019163.01.T01:CDS 35.0%
! TTCTGAGAGATACAATTGTC+AGG - Chr4:15239503-15239522 MsG0480019163.01.T01:intron 35.0%
AACTTCGATGGTTTACTGCT+TGG - Chr4:15239598-15239617 MsG0480019163.01.T01:CDS 40.0%
AATCGAATTCCATAGCCCAT+TGG + Chr4:15239183-15239202 None:intergenic 40.0%
ATCTCCAAATCCAAACAAGC+CGG - Chr4:15239229-15239248 MsG0480019163.01.T01:CDS 40.0%
GGTTTACTGCTTGGAAACAA+AGG - Chr4:15239607-15239626 MsG0480019163.01.T01:CDS 40.0%
! TGATTTTTGTCCCAATGCCT+CGG + Chr4:15239670-15239689 None:intergenic 40.0%
!! CAATCTTAACCAATGGGCTA+TGG - Chr4:15239171-15239190 MsG0480019163.01.T01:CDS 40.0%
!! GTTTTGTTGCTACTGCAGAT+GGG - Chr4:15239404-15239423 MsG0480019163.01.T01:CDS 40.0%
AATGCCTCGGAAACATTAGG+AGG + Chr4:15239657-15239676 None:intergenic 45.0%
ACTTCCTCCTAATGTTTCCG+AGG - Chr4:15239650-15239669 MsG0480019163.01.T01:CDS 45.0%
ATCATTTGCAAGGCACGTCT+TGG + Chr4:15239565-15239584 None:intergenic 45.0%
TCATTTGCAAGGCACGTCTT+GGG + Chr4:15239564-15239583 None:intergenic 45.0%
TCCTAATGTTTCCGAGGCAT+TGG - Chr4:15239656-15239675 MsG0480019163.01.T01:CDS 45.0%
! CCTAATGTTTCCGAGGCATT+GGG - Chr4:15239657-15239676 MsG0480019163.01.T01:CDS 45.0%
! GGCTTGTTTGGATTTGGAGA+TGG + Chr4:15239230-15239249 None:intergenic 45.0%
!! ATAACCGGGGATTTCGAGTT+CGG + Chr4:15239275-15239294 None:intergenic 45.0%
!! TGGTGTCGAGTTCTAAGAAG+TGG + Chr4:15239309-15239328 None:intergenic 45.0%
!!! GGTTTTGTTGCTACTGCAGA+TGG - Chr4:15239403-15239422 MsG0480019163.01.T01:CDS 45.0%
CCCAATGCCTCGGAAACATT+AGG + Chr4:15239660-15239679 None:intergenic 50.0%
GAACTCGAAATCCCCGGTTA+TGG - Chr4:15239274-15239293 MsG0480019163.01.T01:CDS 50.0%
GTGGTCTTCACAGCCATAAC+CGG + Chr4:15239290-15239309 None:intergenic 50.0%
TGGTCTTCACAGCCATAACC+GGG + Chr4:15239289-15239308 None:intergenic 50.0%
!! TAACCGGGGATTTCGAGTTC+GGG + Chr4:15239274-15239293 None:intergenic 50.0%
!! TTTCGAGTTCGGGACCAAGA+CGG + Chr4:15239264-15239283 None:intergenic 50.0%
GGTCTTCACAGCCATAACCG+GGG + Chr4:15239288-15239307 None:intergenic 55.0%
TTCGGGACCAAGACGGATGA+CGG + Chr4:15239257-15239276 None:intergenic 55.0%
GGTCCCGAACTCGAAATCCC+CGG - Chr4:15239268-15239287 MsG0480019163.01.T01:CDS 60.0%
!! GGCGCCGGCTTGTTTGGATT+TGG + Chr4:15239236-15239255 None:intergenic 60.0%
! ACCAAGACGGATGACGGCGC+CGG + Chr4:15239251-15239270 None:intergenic 65.0%
!! GATGACGGCGCCGGCTTGTT+TGG + Chr4:15239242-15239261 None:intergenic 65.0%
GCCGGCGCCGTCATCCGTCT+TGG - Chr4:15239247-15239266 MsG0480019163.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 15239145 15239731 15239145 ID=MsG0480019163.01;Name=MsG0480019163.01
Chr4 mRNA 15239145 15239731 15239145 ID=MsG0480019163.01.T01;Parent=MsG0480019163.01;Name=MsG0480019163.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|167
Chr4 exon 15239538 15239731 15239538 ID=MsG0480019163.01.T01:exon:4947;Parent=MsG0480019163.01.T01
Chr4 exon 15239145 15239454 15239145 ID=MsG0480019163.01.T01:exon:4946;Parent=MsG0480019163.01.T01
Chr4 CDS 15239538 15239731 15239538 ID=MsG0480019163.01.T01:cds;Parent=MsG0480019163.01.T01
Chr4 CDS 15239145 15239454 15239145 ID=MsG0480019163.01.T01:cds;Parent=MsG0480019163.01.T01
Gene Sequence

>MsG0480019163.01.T01

ATGAGGCTCTTGAAGGTAGCTACTTGCAATCTTAACCAATGGGCTATGGAATTCGATTCCAATCTTCTACAAATCAAAGACTCCATCTCCAAATCCAAACAAGCCGGCGCCGTCATCCGTCTTGGTCCCGAACTCGAAATCCCCGGTTATGGCTGTGAAGACCACTTCTTAGAACTCGACACCATTAACCATTCATGGGAATGTTTGAAAGATATATTGGTTGGTGATTGGACTGATAACATAGTGTGCAGTATTGGAATGCCTATTATCAAAGGTTCTGAGAGATACAATTGTCAGGTTTTGTGTTTCAACCGAAAGATTATTATGATACGACCCAAGACGTGCCTTGCAAATGATGATAATTATAGAGAACTTCGATGGTTTACTGCTTGGAAACAAAGGGACCAACTTTATGATTTTCAACTTCCTCCTAATGTTTCCGAGGCATTGGGACAAAAATCAGTTCCTTTTGGTTATGGTTTTGTTAAGTTTCAAGACACGTAA

Protein sequence

>MsG0480019163.01.T01

MRLLKVATCNLNQWAMEFDSNLLQIKDSISKSKQAGAVIRLGPELEIPGYGCEDHFLELDTINHSWECLKDILVGDWTDNIVCSIGMPIIKGSERYNCQVLCFNRKIIMIRPKTCLANDDNYRELRWFTAWKQRDQLYDFQLPPNVSEALGQKSVPFGYGFVKFQDT*