Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019280.01.T01 | XP_039689730.1 | 76.86 | 121 | 28 | 0 | 1 | 121 | 1 | 121 | 6.91E-58 | 186 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019280.01.T01 | Q39950 | 47.917 | 96 | 48 | 2 | 18 | 113 | 15 | 108 | 4.39E-25 | 95.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019280.01.T01 | A0A396I196 | 76.860 | 121 | 28 | 0 | 1 | 121 | 1 | 121 | 3.30e-58 | 186 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480019280.01 | MsG0680031468.01 | 0.801207 | 1.003872e-48 | 4.766270e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019280.01.T01 | MTR_4g028190 | 86.667 | 105 | 14 | 0 | 1 | 105 | 1 | 105 | 1.29e-61 | 185 |
| MsG0480019280.01.T01 | MTR_7g072930 | 48.571 | 105 | 52 | 2 | 1 | 105 | 1 | 103 | 1.41e-29 | 105 |
| MsG0480019280.01.T01 | MTR_7g073170 | 46.226 | 106 | 54 | 3 | 1 | 106 | 1 | 103 | 2.72e-27 | 98.2 |
| MsG0480019280.01.T01 | MTR_7g073150 | 44.762 | 105 | 56 | 2 | 1 | 105 | 1 | 103 | 9.77e-27 | 97.4 |
| MsG0480019280.01.T01 | MTR_7g072830 | 41.228 | 114 | 64 | 3 | 1 | 113 | 1 | 112 | 2.09e-25 | 93.6 |
| MsG0480019280.01.T01 | MTR_4g027840 | 46.078 | 102 | 53 | 2 | 5 | 106 | 1 | 100 | 2.93e-24 | 91.3 |
| MsG0480019280.01.T01 | MTR_7g073120 | 42.991 | 107 | 59 | 2 | 1 | 106 | 1 | 106 | 4.13e-24 | 91.3 |
| MsG0480019280.01.T01 | MTR_4g027800 | 45.098 | 102 | 54 | 2 | 5 | 106 | 1 | 100 | 5.10e-24 | 89.7 |
| MsG0480019280.01.T01 | MTR_4g428370 | 44.118 | 102 | 55 | 2 | 5 | 106 | 58 | 157 | 2.69e-23 | 89.7 |
| MsG0480019280.01.T01 | MTR_7g072730 | 45.192 | 104 | 54 | 3 | 1 | 103 | 1 | 102 | 6.52e-22 | 85.9 |
| MsG0480019280.01.T01 | MTR_7g072900 | 43.810 | 105 | 57 | 2 | 1 | 105 | 1 | 103 | 1.81e-21 | 83.6 |
| MsG0480019280.01.T01 | MTR_7g073130 | 43.810 | 105 | 56 | 3 | 1 | 105 | 1 | 102 | 3.09e-21 | 83.2 |
| MsG0480019280.01.T01 | MTR_7g072980 | 43.651 | 126 | 67 | 4 | 1 | 124 | 1 | 124 | 3.13e-21 | 83.6 |
| MsG0480019280.01.T01 | MTR_7g072993 | 40.945 | 127 | 70 | 4 | 1 | 124 | 1 | 125 | 1.02e-20 | 82.0 |
| MsG0480019280.01.T01 | MTR_4g029210 | 40.625 | 96 | 55 | 2 | 8 | 103 | 4 | 97 | 3.07e-18 | 75.1 |
| MsG0480019280.01.T01 | MTR_4g029350 | 38.614 | 101 | 61 | 1 | 5 | 105 | 1 | 100 | 2.27e-17 | 72.8 |
| MsG0480019280.01.T01 | MTR_1g040335 | 43.396 | 106 | 56 | 4 | 1 | 105 | 1 | 103 | 3.22e-17 | 72.4 |
| MsG0480019280.01.T01 | MTR_7g073100 | 41.346 | 104 | 59 | 2 | 1 | 104 | 1 | 102 | 4.01e-17 | 73.2 |
| MsG0480019280.01.T01 | MTR_7g073060 | 43.269 | 104 | 57 | 2 | 1 | 104 | 1 | 102 | 6.71e-17 | 71.6 |
| MsG0480019280.01.T01 | MTR_7g451500 | 37.931 | 87 | 49 | 1 | 19 | 105 | 2 | 83 | 7.47e-16 | 68.6 |
| MsG0480019280.01.T01 | MTR_4g029390 | 38.235 | 102 | 62 | 1 | 5 | 106 | 1 | 101 | 8.63e-16 | 68.9 |
| MsG0480019280.01.T01 | MTR_7g072960 | 37.736 | 106 | 50 | 3 | 1 | 106 | 1 | 90 | 1.11e-15 | 68.9 |
| MsG0480019280.01.T01 | MTR_4g028180 | 33.333 | 111 | 69 | 2 | 1 | 106 | 1 | 111 | 9.85e-15 | 66.2 |
| MsG0480019280.01.T01 | MTR_7g073030 | 35.294 | 102 | 65 | 1 | 3 | 104 | 2 | 102 | 3.46e-14 | 65.9 |
| MsG0480019280.01.T01 | MTR_7g072987 | 40.244 | 82 | 48 | 1 | 40 | 120 | 67 | 148 | 1.61e-13 | 63.9 |
| MsG0480019280.01.T01 | MTR_2g062600 | 30.693 | 101 | 69 | 1 | 13 | 113 | 8 | 107 | 1.23e-12 | 60.5 |
| MsG0480019280.01.T01 | MTR_1g040330 | 43.396 | 106 | 56 | 4 | 1 | 105 | 1 | 103 | 2.02e-12 | 60.1 |
| MsG0480019280.01.T01 | MTR_7g072810 | 37.383 | 107 | 58 | 4 | 1 | 106 | 1 | 99 | 3.81e-12 | 59.7 |
| MsG0480019280.01.T01 | MTR_7g072760 | 40.000 | 85 | 46 | 4 | 1 | 82 | 1 | 83 | 9.93e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019280.01.T01 | AT5G59320 | 40.777 | 103 | 57 | 2 | 4 | 106 | 2 | 100 | 7.52e-21 | 81.6 |
| MsG0480019280.01.T01 | AT2G15050 | 44.660 | 103 | 51 | 4 | 7 | 105 | 4 | 104 | 4.20e-20 | 79.7 |
| MsG0480019280.01.T01 | AT2G15050 | 44.660 | 103 | 51 | 4 | 7 | 105 | 4 | 104 | 5.15e-20 | 79.7 |
| MsG0480019280.01.T01 | AT2G38540 | 46.078 | 102 | 51 | 4 | 7 | 106 | 4 | 103 | 5.29e-20 | 79.7 |
| MsG0480019280.01.T01 | AT2G15050 | 44.660 | 103 | 51 | 4 | 7 | 105 | 4 | 104 | 7.55e-20 | 79.3 |
| MsG0480019280.01.T01 | AT5G59310 | 38.462 | 104 | 55 | 4 | 4 | 106 | 2 | 97 | 3.23e-17 | 72.4 |
| MsG0480019280.01.T01 | AT5G01870 | 39.241 | 79 | 45 | 2 | 29 | 105 | 23 | 100 | 2.79e-15 | 67.4 |
| MsG0480019280.01.T01 | AT3G51600 | 40.404 | 99 | 55 | 4 | 7 | 103 | 4 | 100 | 8.65e-15 | 66.2 |
| MsG0480019280.01.T01 | AT2G38530 | 39.394 | 99 | 56 | 4 | 7 | 103 | 4 | 100 | 8.84e-15 | 66.2 |
| MsG0480019280.01.T01 | AT4G33355 | 34.043 | 94 | 61 | 1 | 13 | 106 | 12 | 104 | 1.70e-14 | 65.5 |
| MsG0480019280.01.T01 | AT4G33355 | 34.043 | 94 | 61 | 1 | 13 | 106 | 12 | 104 | 1.72e-14 | 65.5 |
| MsG0480019280.01.T01 | AT3G51590 | 37.113 | 97 | 58 | 3 | 10 | 106 | 8 | 101 | 2.33e-14 | 65.1 |
| MsG0480019280.01.T01 | AT3G08770 | 46.341 | 82 | 35 | 4 | 29 | 105 | 20 | 97 | 1.39e-12 | 60.5 |
| MsG0480019280.01.T01 | AT3G08770 | 46.341 | 82 | 35 | 4 | 29 | 105 | 20 | 97 | 1.57e-12 | 60.5 |
| MsG0480019280.01.T01 | AT2G18370 | 36.709 | 79 | 48 | 2 | 12 | 88 | 6 | 84 | 1.45e-11 | 57.8 |
Find 41 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCGATCCATGGACTGAATA+TGG | 0.258365 | 4:-17660302 | MsG0480019280.01.T01:CDS |
| TCTTCAAGTAGAAACAAAAT+TGG | 0.372504 | 4:-17660464 | MsG0480019280.01.T01:CDS |
| GTGGAGGGACCAAGAGTATA+AGG | 0.378943 | 4:+17660239 | None:intergenic |
| GTTAACTTGCCTTATACTCT+TGG | 0.381008 | 4:-17660248 | MsG0480019280.01.T01:CDS |
| GGCGTCCTGACGATCAGATT+TGG | 0.403991 | 4:+17660351 | None:intergenic |
| GAGCAGCAGCTACAGCTTAG+AGG | 0.404948 | 4:-17659651 | MsG0480019280.01.T01:CDS |
| GGTCACGGCTTGGTCGTGTA+TGG | 0.419973 | 4:+17660372 | None:intergenic |
| GTCGGGTATGTGACGGGAAA+TGG | 0.442263 | 4:-17660433 | MsG0480019280.01.T01:CDS |
| GCTAACATCCCTGGCAGATG+TGG | 0.442530 | 4:-17660271 | MsG0480019280.01.T01:CDS |
| ACCGCTGCTTCCGCGATCCA+TGG | 0.463522 | 4:-17660313 | MsG0480019280.01.T01:CDS |
| AGCAGCAGCTACAGCTTAGA+GGG | 0.465447 | 4:-17659650 | MsG0480019280.01.T01:CDS |
| CGTGACCAAATCTGATCGTC+AGG | 0.473248 | 4:-17660356 | MsG0480019280.01.T01:CDS |
| CAAGTTAACCCACATCTGCC+AGG | 0.476525 | 4:+17660262 | None:intergenic |
| GTCCCTCAAGCGTGCTGCGA+TGG | 0.482531 | 4:-17660403 | MsG0480019280.01.T01:CDS |
| CTGAGAGCATCAAGAGTTTG+TGG | 0.484983 | 4:+17660490 | None:intergenic |
| AGCGTGCTGCGATGGTGTTA+AGG | 0.499638 | 4:-17660395 | MsG0480019280.01.T01:CDS |
| ATTCAATATGTCTAGCTCAA+TGG | 0.509668 | 4:-17660566 | None:intergenic |
| CAAAATTGGTACCATGCGTC+GGG | 0.511654 | 4:-17660450 | MsG0480019280.01.T01:CDS |
| CACCATCGCAGCACGCTTGA+GGG | 0.512663 | 4:+17660401 | None:intergenic |
| GATGTTGTTTATCAAGTTTG+AGG | 0.521818 | 4:-17659621 | None:intergenic |
| ACACCATCGCAGCACGCTTG+AGG | 0.527998 | 4:+17660400 | None:intergenic |
| CCATGCGTCGGGTATGTGAC+GGG | 0.528316 | 4:-17660439 | MsG0480019280.01.T01:CDS |
| GATCAGATTTGGTCACGGCT+TGG | 0.540631 | 4:+17660362 | None:intergenic |
| GAGTGTCCATATTCAGTCCA+TGG | 0.541841 | 4:+17660296 | None:intergenic |
| GACACTCTTGCTAACATCCC+TGG | 0.549472 | 4:-17660280 | MsG0480019280.01.T01:CDS |
| ACAAAATTGGTACCATGCGT+CGG | 0.556839 | 4:-17660451 | MsG0480019280.01.T01:CDS |
| AGCGGTCTTGATACACCTAC+AGG | 0.560408 | 4:+17660330 | None:intergenic |
| CTTGACAATGATGATGTGCA+TGG | 0.561594 | 4:-17660524 | MsG0480019280.01.T01:CDS |
| TCCATGGATCGCGGAAGCAG+CGG | 0.577186 | 4:+17660312 | None:intergenic |
| ACCATGCGTCGGGTATGTGA+CGG | 0.581360 | 4:-17660440 | MsG0480019280.01.T01:CDS |
| CTGATCGTCAGGACGCCTGT+AGG | 0.587938 | 4:-17660345 | MsG0480019280.01.T01:CDS |
| TAGCTCAATGGTGTTGGTGA+AGG | 0.594904 | 4:-17660554 | MsG0480019280.01.T01:CDS |
| CTAACATCCCTGGCAGATGT+GGG | 0.595510 | 4:-17660270 | MsG0480019280.01.T01:CDS |
| CCCGTCACATACCCGACGCA+TGG | 0.612944 | 4:+17660439 | None:intergenic |
| TATGTCTAGCTCAATGGTGT+TGG | 0.617955 | 4:-17660560 | MsG0480019280.01.T01:CDS |
| AAGTTAACCCACATCTGCCA+GGG | 0.625369 | 4:+17660263 | None:intergenic |
| GGGTATGTGACGGGAAATGG+TGG | 0.645108 | 4:-17660430 | MsG0480019280.01.T01:CDS |
| CATATTCAGTCCATGGATCG+CGG | 0.645471 | 4:+17660303 | None:intergenic |
| CTGACGATCAGATTTGGTCA+CGG | 0.647791 | 4:+17660357 | None:intergenic |
| CAGCAGCTACAGCTTAGAGG+GGG | 0.702353 | 4:-17659648 | MsG0480019280.01.T01:CDS |
| GCAGCAGCTACAGCTTAGAG+GGG | 0.718284 | 4:-17659649 | MsG0480019280.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAAAAGTTATCTTAATA+AGG | + | Chr4:17660492-17660511 | None:intergenic | 10.0% |
| !! | TAAAAGAAAAAAAAAAAAAG+AGG | - | Chr4:17660337-17660356 | MsG0480019280.01.T01:CDS | 10.0% |
| !! | GTAACTTTATCATAATCAAT+GGG | + | Chr4:17659995-17660014 | None:intergenic | 20.0% |
| !! | TAACTTTATCATAATCAATG+GGG | + | Chr4:17659994-17660013 | None:intergenic | 20.0% |
| !! | TGTAACTTTATCATAATCAA+TGG | + | Chr4:17659996-17660015 | None:intergenic | 20.0% |
| ! | AAAAAAATGAAATGCGTTGA+TGG | - | Chr4:17660111-17660130 | MsG0480019280.01.T01:intron | 25.0% |
| ! | TCTTCAAGTAGAAACAAAAT+TGG | - | Chr4:17659718-17659737 | MsG0480019280.01.T01:intron | 25.0% |
| !! | AACTTTTTATTGTCAAATGC+AGG | - | Chr4:17660501-17660520 | MsG0480019280.01.T01:CDS | 25.0% |
| ATCTCTTATGTGATTTGAGT+TGG | + | Chr4:17660385-17660404 | None:intergenic | 30.0% | |
| !! | ATGATGATGTGCATGGTTTT+AGG | - | Chr4:17659665-17659684 | MsG0480019280.01.T01:intron | 35.0% |
| !! | GTTAACTTGCCTTATACTCT+TGG | - | Chr4:17659934-17659953 | MsG0480019280.01.T01:intron | 35.0% |
| !! | TGATGATGTGCATGGTTTTA+GGG | - | Chr4:17659666-17659685 | MsG0480019280.01.T01:intron | 35.0% |
| ACAAAATTGGTACCATGCGT+CGG | - | Chr4:17659731-17659750 | MsG0480019280.01.T01:intron | 40.0% | |
| ! | CTTGACAATGATGATGTGCA+TGG | - | Chr4:17659658-17659677 | MsG0480019280.01.T01:CDS | 40.0% |
| AAGTTAACCCACATCTGCCA+GGG | + | Chr4:17659922-17659941 | None:intergenic | 45.0% | |
| CAAAATTGGTACCATGCGTC+GGG | - | Chr4:17659732-17659751 | MsG0480019280.01.T01:intron | 45.0% | |
| CATATTCAGTCCATGGATCG+CGG | + | Chr4:17659882-17659901 | None:intergenic | 45.0% | |
| GAGTGTCCATATTCAGTCCA+TGG | + | Chr4:17659889-17659908 | None:intergenic | 45.0% | |
| ! | ATACTTTTCGCAGCTTGTGG+AGG | + | Chr4:17659962-17659981 | None:intergenic | 45.0% |
| ! | CTGACGATCAGATTTGGTCA+CGG | + | Chr4:17659828-17659847 | None:intergenic | 45.0% |
| ! | GACATACTTTTCGCAGCTTG+TGG | + | Chr4:17659965-17659984 | None:intergenic | 45.0% |
| ! | TACTTTTCGCAGCTTGTGGA+GGG | + | Chr4:17659961-17659980 | None:intergenic | 45.0% |
| !! | CTGAGAGCATCAAGAGTTTG+TGG | + | Chr4:17659695-17659714 | None:intergenic | 45.0% |
| !! | TAGCTCAATGGTGTTGGTGA+AGG | - | Chr4:17659628-17659647 | MsG0480019280.01.T01:CDS | 45.0% |
| AGCAGCAGCTACAGCTTAGA+GGG | - | Chr4:17660532-17660551 | MsG0480019280.01.T01:CDS | 50.0% | |
| AGCGGTCTTGATACACCTAC+AGG | + | Chr4:17659855-17659874 | None:intergenic | 50.0% | |
| CAAGTTAACCCACATCTGCC+AGG | + | Chr4:17659923-17659942 | None:intergenic | 50.0% | |
| CGTGACCAAATCTGATCGTC+AGG | - | Chr4:17659826-17659845 | MsG0480019280.01.T01:intron | 50.0% | |
| CTAACATCCCTGGCAGATGT+GGG | - | Chr4:17659912-17659931 | MsG0480019280.01.T01:intron | 50.0% | |
| GACACTCTTGCTAACATCCC+TGG | - | Chr4:17659902-17659921 | MsG0480019280.01.T01:intron | 50.0% | |
| ! | CGCGATCCATGGACTGAATA+TGG | - | Chr4:17659880-17659899 | MsG0480019280.01.T01:intron | 50.0% |
| ! | GATCAGATTTGGTCACGGCT+TGG | + | Chr4:17659823-17659842 | None:intergenic | 50.0% |
| ! | GTGGAGGGACCAAGAGTATA+AGG | + | Chr4:17659946-17659965 | None:intergenic | 50.0% |
| ACCATGCGTCGGGTATGTGA+CGG | - | Chr4:17659742-17659761 | MsG0480019280.01.T01:intron | 55.0% | |
| CAGCAGCTACAGCTTAGAGG+GGG | - | Chr4:17660534-17660553 | MsG0480019280.01.T01:CDS | 55.0% | |
| GAGCAGCAGCTACAGCTTAG+AGG | - | Chr4:17660531-17660550 | MsG0480019280.01.T01:CDS | 55.0% | |
| GCAGCAGCTACAGCTTAGAG+GGG | - | Chr4:17660533-17660552 | MsG0480019280.01.T01:CDS | 55.0% | |
| GCTAACATCCCTGGCAGATG+TGG | - | Chr4:17659911-17659930 | MsG0480019280.01.T01:intron | 55.0% | |
| GGCGTCCTGACGATCAGATT+TGG | + | Chr4:17659834-17659853 | None:intergenic | 55.0% | |
| ! | AGCGTGCTGCGATGGTGTTA+AGG | - | Chr4:17659787-17659806 | MsG0480019280.01.T01:intron | 55.0% |
| ! | GGGTATGTGACGGGAAATGG+TGG | - | Chr4:17659752-17659771 | MsG0480019280.01.T01:intron | 55.0% |
| ! | GTCGGGTATGTGACGGGAAA+TGG | - | Chr4:17659749-17659768 | MsG0480019280.01.T01:intron | 55.0% |
| ACACCATCGCAGCACGCTTG+AGG | + | Chr4:17659785-17659804 | None:intergenic | 60.0% | |
| CACCATCGCAGCACGCTTGA+GGG | + | Chr4:17659784-17659803 | None:intergenic | 60.0% | |
| CCATGCGTCGGGTATGTGAC+GGG | - | Chr4:17659743-17659762 | MsG0480019280.01.T01:intron | 60.0% | |
| CTGATCGTCAGGACGCCTGT+AGG | - | Chr4:17659837-17659856 | MsG0480019280.01.T01:intron | 60.0% | |
| TCCATGGATCGCGGAAGCAG+CGG | + | Chr4:17659873-17659892 | None:intergenic | 60.0% | |
| !! | GGTCACGGCTTGGTCGTGTA+TGG | + | Chr4:17659813-17659832 | None:intergenic | 60.0% |
| ACCGCTGCTTCCGCGATCCA+TGG | - | Chr4:17659869-17659888 | MsG0480019280.01.T01:intron | 65.0% | |
| CCCGTCACATACCCGACGCA+TGG | + | Chr4:17659746-17659765 | None:intergenic | 65.0% | |
| GTCCCTCAAGCGTGCTGCGA+TGG | - | Chr4:17659779-17659798 | MsG0480019280.01.T01:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 17659623 | 17660581 | 17659623 | ID=MsG0480019280.01;Name=MsG0480019280.01 |
| Chr4 | mRNA | 17659623 | 17660581 | 17659623 | ID=MsG0480019280.01.T01;Parent=MsG0480019280.01;Name=MsG0480019280.01.T01;_AED=0.50;_eAED=0.57;_QI=0|0|0|1|1|1|2|0|137 |
| Chr4 | exon | 17660227 | 17660581 | 17660227 | ID=MsG0480019280.01.T01:exon:5483;Parent=MsG0480019280.01.T01 |
| Chr4 | exon | 17659623 | 17659681 | 17659623 | ID=MsG0480019280.01.T01:exon:5482;Parent=MsG0480019280.01.T01 |
| Chr4 | CDS | 17660227 | 17660581 | 17660227 | ID=MsG0480019280.01.T01:cds;Parent=MsG0480019280.01.T01 |
| Chr4 | CDS | 17659623 | 17659681 | 17659623 | ID=MsG0480019280.01.T01:cds;Parent=MsG0480019280.01.T01 |
| Gene Sequence |
| Protein sequence |
