AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031468.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031468.01.T01 MTR_6g023910 64.444 270 24 2 1 198 1 270 2.32e-111 319
MsG0680031468.01.T01 MTR_6g023610 60.595 269 34 2 1 197 1 269 3.17e-99 288
MsG0680031468.01.T01 MTR_6g023600 53.668 259 54 2 1 193 1 259 1.97e-84 250
MsG0680031468.01.T01 MTR_6g023600 52.453 265 54 2 1 193 1 265 1.60e-83 248
MsG0680031468.01.T01 MTR_6g023590 52.075 265 55 2 1 193 1 265 9.49e-83 246
MsG0680031468.01.T01 MTR_6g023950 52.490 261 52 2 1 189 1 261 2.96e-81 243
MsG0680031468.01.T01 MTR_6g023990 51.880 266 55 3 1 193 1 266 4.23e-79 237
MsG0680031468.01.T01 MTR_6g023940 65.806 155 37 3 1 142 1 152 2.58e-58 180
MsG0680031468.01.T01 MTR_7g110160 38.961 231 87 3 12 189 5 234 9.05e-45 149
MsG0680031468.01.T01 MTR_2g090165 37.349 249 84 3 12 189 5 252 2.87e-44 148
MsG0680031468.01.T01 MTR_7g110130 36.800 250 85 3 12 189 5 253 2.87e-42 143
MsG0680031468.01.T01 MTR_7g110140 36.653 251 87 4 10 189 3 252 4.44e-42 142
MsG0680031468.01.T01 MTR_6g035395 38.136 236 87 3 12 189 4 238 1.46e-40 138
MsG0680031468.01.T01 MTR_7g105460 32.692 260 103 4 1 189 1 259 3.53e-36 127
MsG0680031468.01.T01 MTR_4g052300 32.549 255 89 6 8 187 3 249 5.69e-29 108
MsG0680031468.01.T01 MTR_7g105580 32.328 232 101 4 12 189 7 236 7.41e-29 108
MsG0680031468.01.T01 MTR_4g052320 31.496 254 98 5 8 189 3 252 1.78e-27 105
MsG0680031468.01.T01 MTR_4g052350 30.980 255 99 5 12 194 13 262 1.93e-27 105
MsG0680031468.01.T01 MTR_4g078630 33.083 266 95 6 12 198 18 279 4.10e-27 104
MsG0680031468.01.T01 MTR_7g071680 32.246 276 89 9 12 194 28 298 5.23e-27 104
MsG0680031468.01.T01 MTR_4g052340 32.143 252 88 6 12 188 13 256 3.53e-26 101
MsG0680031468.01.T01 MTR_7g105570 32.487 197 112 4 13 189 21 216 6.44e-23 92.4
MsG0680031468.01.T01 MTR_7g107670 26.792 265 114 6 7 194 27 288 1.15e-20 87.4
MsG0680031468.01.T01 MTR_4g084800 56.944 72 30 1 1 72 1 71 1.42e-20 84.0
MsG0680031468.01.T01 MTR_3g020670 28.137 263 110 5 12 196 15 276 1.51e-20 87.0
MsG0680031468.01.T01 MTR_7g105560 59.701 67 26 1 1 67 1 66 2.81e-18 78.2
MsG0680031468.01.T01 MTR_7g083870 27.308 260 110 8 12 194 34 291 7.58e-18 80.1
MsG0680031468.01.T01 MTR_7g105590 30.159 189 58 5 12 128 7 193 2.31e-16 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031468.01.T01 AT2G47140 38.956 249 80 3 12 189 5 252 6.46e-45 150
MsG0680031468.01.T01 AT3G29250 34.646 254 94 4 7 189 36 288 2.57e-36 129
MsG0680031468.01.T01 AT3G29250 34.127 252 94 3 9 189 2 252 2.62e-36 127
MsG0680031468.01.T01 AT2G47130 35.547 256 84 6 9 189 2 251 1.91e-35 125
MsG0680031468.01.T01 AT2G47120 35.857 251 89 6 9 189 2 250 6.41e-35 124
MsG0680031468.01.T01 AT3G29250 34.375 256 94 5 7 189 36 290 1.84e-34 124
MsG0680031468.01.T01 AT2G47120 35.887 248 87 6 12 189 6 251 3.40e-34 122
MsG0680031468.01.T01 AT3G29260 35.743 249 87 4 12 189 5 251 7.21e-33 119
MsG0680031468.01.T01 AT3G26760 29.478 268 108 7 6 194 29 294 3.93e-28 107
MsG0680031468.01.T01 AT3G42960 30.769 273 105 9 6 198 1 269 2.11e-23 94.4
MsG0680031468.01.T01 AT3G26770 28.626 262 102 7 12 193 40 296 2.65e-21 89.4
MsG0680031468.01.T01 AT1G52340 29.615 260 99 7 12 189 17 274 1.79e-20 87.0
MsG0680031468.01.T01 AT4G03140 28.077 260 107 6 12 194 77 333 2.30e-20 87.4
MsG0680031468.01.T01 AT3G51680 32.903 155 70 3 71 194 148 299 8.51e-17 77.0

Find 52 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGATGGTAGCTATGTTTC+TGG 0.186717 6:-20872591 MsG0680031468.01.T01:CDS
CATTCACTAACAACAATCTT+AGG 0.241211 6:-20873624 MsG0680031468.01.T01:CDS
TACCATGCGTTCACCTTGTT+CGG 0.342845 6:+20873531 None:intergenic
GCCAATGCCGCTGGCACCTC+CGG 0.364087 6:+20873576 None:intergenic
AGTGAATGTAGGTGCATCTA+TGG 0.374597 6:+20873452 None:intergenic
GGCAGATGATGTGTTGTTCT+TGG 0.390117 6:-20872655 MsG0680031468.01.T01:CDS
CTTGATTTCCCAACTCGTCT+TGG 0.391032 6:+20873494 None:intergenic
CACTGACCTTGATCTTCTTG+TGG 0.403730 6:-20872610 MsG0680031468.01.T01:CDS
CGTCTCTTTGCCAATGCCGC+TGG 0.405832 6:+20873567 None:intergenic
ACCGGAGGTGCCAGCGGCAT+TGG 0.425693 6:-20873577 MsG0680031468.01.T01:CDS
AACTTGCAAAGCATGGGATA+AGG 0.445636 6:-20872812 MsG0680031468.01.T01:CDS
AACAACAATCTTAGGTTAGC+AGG 0.450218 6:-20873616 MsG0680031468.01.T01:CDS
TTGTTCTCAGTTAACATTCG+AGG 0.461567 6:-20873000 MsG0680031468.01.T01:intron
GTGTGCACGGCTAGCGTCGC+CGG 0.471191 6:-20872913 MsG0680031468.01.T01:intron
GCCGACATCCAAGACGAGTT+GGG 0.474113 6:-20873502 MsG0680031468.01.T01:CDS
CAATGGTGGACGGGTGCGTG+AGG 0.474303 6:-20872945 MsG0680031468.01.T01:CDS
GTGAATTGTGTATCACCAAA+TGG 0.474979 6:-20872789 MsG0680031468.01.T01:CDS
GGAGAATACAAGAGGTTGGA+AGG 0.477377 6:-20872702 MsG0680031468.01.T01:CDS
TCCCAACTCGTCTTGGATGT+CGG 0.482522 6:+20873501 None:intergenic
CTCAGTTAACATTCGAGGAA+TGG 0.485569 6:-20872995 MsG0680031468.01.T01:intron
CGCCGACATCCAAGACGAGT+TGG 0.490614 6:-20873503 MsG0680031468.01.T01:CDS
ACTTGCAAAGCATGGGATAA+GGG 0.492055 6:-20872811 MsG0680031468.01.T01:CDS
ACATGTAAATTGCTAGATGC+AGG 0.492615 6:-20872750 MsG0680031468.01.T01:CDS
TGGATGGTAGCTATGTTTCT+GGG 0.493936 6:-20872590 MsG0680031468.01.T01:CDS
GGCAAAATAGCCATCGTCAC+CGG 0.494254 6:-20873595 MsG0680031468.01.T01:CDS
AATGGTGTTGCTAATCCATT+TGG 0.497446 6:+20872774 None:intergenic
ATGGTGGACGGGTGCGTGAG+GGG 0.498110 6:-20872943 MsG0680031468.01.T01:CDS
AATGGTGGACGGGTGCGTGA+GGG 0.501756 6:-20872944 MsG0680031468.01.T01:CDS
AATCAAGTTGCTGCATCCAT+TGG 0.502662 6:-20873478 MsG0680031468.01.T01:CDS
TCTAGCAATTTACATGTCAA+TGG 0.504616 6:+20872756 None:intergenic
AATAATAGGGTTGATGCGTT+CGG 0.520347 6:-20872844 MsG0680031468.01.T01:intron
GAGGGGTAGCATTGTGTGCA+CGG 0.524508 6:-20872926 MsG0680031468.01.T01:CDS
GACCTTGATCTTCTTGTGGA+TGG 0.528900 6:-20872606 MsG0680031468.01.T01:CDS
AGTGTGCAACTTGCAAAGCA+TGG 0.534452 6:-20872819 MsG0680031468.01.T01:CDS
GTGCATGTCTTTGCCGAACA+AGG 0.542837 6:-20873544 MsG0680031468.01.T01:CDS
CAAGTTGCTGCATCCATTGG+AGG 0.543473 6:-20873475 MsG0680031468.01.T01:CDS
TGGCACGTGCAATGGTGGAC+GGG 0.544118 6:-20872954 MsG0680031468.01.T01:CDS
GTGGCACGTGCAATGGTGGA+CGG 0.549228 6:-20872955 MsG0680031468.01.T01:CDS
CAGCGGCATTGGCAAAGAGA+CGG 0.557214 6:-20873566 MsG0680031468.01.T01:CDS
ATACAAGAGGTTGGAAGGAG+TGG 0.561860 6:-20872697 MsG0680031468.01.T01:CDS
GAAACATGTGGCACGTGCAA+TGG 0.568899 6:-20872962 MsG0680031468.01.T01:CDS
GTGTGCAACTTGCAAAGCAT+GGG 0.574953 6:-20872818 MsG0680031468.01.T01:CDS
GCTGGCACCTCCGGTGACGA+TGG 0.584463 6:+20873585 None:intergenic
ACATGTGGCACGTGCAATGG+TGG 0.588988 6:-20872959 MsG0680031468.01.T01:CDS
ATCGTCACCGGAGGTGCCAG+CGG 0.590387 6:-20873583 MsG0680031468.01.T01:CDS
TACCATCCACAAGAAGATCA+AGG 0.590935 6:+20872604 None:intergenic
GGTGCATCTATGGCCTCCAA+TGG 0.599803 6:+20873462 None:intergenic
TGCCGAACAAGGTGAACGCA+TGG 0.615018 6:-20873533 MsG0680031468.01.T01:CDS
TGCTACGTCACAGTGAATGT+AGG 0.629129 6:+20873441 None:intergenic
AAAATAGCCATCGTCACCGG+AGG 0.676812 6:-20873592 MsG0680031468.01.T01:CDS
GGCATTGTGCGTGAAACATG+TGG 0.681076 6:-20872974 MsG0680031468.01.T01:CDS
GGTTCTCAACACTAAACATG+TGG 0.686817 6:-20872676 MsG0680031468.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATATTATGTTTAGCAATGCT+GGG - Chr6:20873125-20873144 MsG0680031468.01.T01:intron 25.0%
! CACAAATGATTCATGATATT+AGG - Chr6:20872955-20872974 MsG0680031468.01.T01:CDS 25.0%
!!! AAGACAGTTGATTTGATTTT+TGG - Chr6:20873483-20873502 MsG0680031468.01.T01:CDS 25.0%
AACATAATATCTACCTGTTG+CGG + Chr6:20873115-20873134 None:intergenic 30.0%
CATTCACTAACAACAATCTT+AGG - Chr6:20872582-20872601 MsG0680031468.01.T01:CDS 30.0%
GAATGTTAACTGAGAACAAA+TGG + Chr6:20873205-20873224 None:intergenic 30.0%
GATATTATGTTTAGCAATGC+TGG - Chr6:20873124-20873143 MsG0680031468.01.T01:intron 30.0%
TCTAGCAATTTACATGTCAA+TGG + Chr6:20873453-20873472 None:intergenic 30.0%
!! TGATTTTTGGAGAATACAAG+AGG - Chr6:20873496-20873515 MsG0680031468.01.T01:CDS 30.0%
AACAACAATCTTAGGTTAGC+AGG - Chr6:20872590-20872609 MsG0680031468.01.T01:CDS 35.0%
AATGTCGAAGCGCAATAATA+GGG - Chr6:20873349-20873368 MsG0680031468.01.T01:intron 35.0%
ACATGTAAATTGCTAGATGC+AGG - Chr6:20873456-20873475 MsG0680031468.01.T01:CDS 35.0%
CAATCGACAGTCAATACTTA+TGG - Chr6:20872815-20872834 MsG0680031468.01.T01:CDS 35.0%
GTGAATTGTGTATCACCAAA+TGG - Chr6:20873417-20873436 MsG0680031468.01.T01:intron 35.0%
TAATGTCGAAGCGCAATAAT+AGG - Chr6:20873348-20873367 MsG0680031468.01.T01:intron 35.0%
TCACACAACATATCTATCTC+TGG + Chr6:20873059-20873078 None:intergenic 35.0%
TTGTTCTCAGTTAACATTCG+AGG - Chr6:20873206-20873225 MsG0680031468.01.T01:intron 35.0%
!! AATAATAGGGTTGATGCGTT+CGG - Chr6:20873362-20873381 MsG0680031468.01.T01:intron 35.0%
!! AATGGTGTTGCTAATCCATT+TGG + Chr6:20873435-20873454 None:intergenic 35.0%
!! TTTTGGAGAATACAAGAGGT+TGG - Chr6:20873500-20873519 MsG0680031468.01.T01:CDS 35.0%
!!! AGTTATTTCTTTTCCGCAAC+AGG - Chr6:20873099-20873118 MsG0680031468.01.T01:intron 35.0%
AACTTGCAAAGCATGGGATA+AGG - Chr6:20873394-20873413 MsG0680031468.01.T01:intron 40.0%
ACTTGCAAAGCATGGGATAA+GGG - Chr6:20873395-20873414 MsG0680031468.01.T01:intron 40.0%
AGTGAATGTAGGTGCATCTA+TGG + Chr6:20872757-20872776 None:intergenic 40.0%
CTCAGTTAACATTCGAGGAA+TGG - Chr6:20873211-20873230 MsG0680031468.01.T01:intron 40.0%
GCGCTTCGACATTATGTAAT+CGG + Chr6:20873342-20873361 None:intergenic 40.0%
GGTTCTCAACACTAAACATG+TGG - Chr6:20873530-20873549 MsG0680031468.01.T01:CDS 40.0%
TACCATCCACAAGAAGATCA+AGG + Chr6:20873605-20873624 None:intergenic 40.0%
TCCAACACAGTTTGATCAGA+TGG + Chr6:20873160-20873179 None:intergenic 40.0%
TCCATCTGATCAAACTGTGT+TGG - Chr6:20873156-20873175 MsG0680031468.01.T01:intron 40.0%
TGGATGGTAGCTATGTTTCT+GGG - Chr6:20873616-20873635 MsG0680031468.01.T01:CDS 40.0%
! AATCAAGTTGCTGCATCCAT+TGG - Chr6:20872728-20872747 MsG0680031468.01.T01:CDS 40.0%
AGTGTGCAACTTGCAAAGCA+TGG - Chr6:20873387-20873406 MsG0680031468.01.T01:intron 45.0%
ATACAAGAGGTTGGAAGGAG+TGG - Chr6:20873509-20873528 MsG0680031468.01.T01:CDS 45.0%
CACTGACCTTGATCTTCTTG+TGG - Chr6:20873596-20873615 MsG0680031468.01.T01:CDS 45.0%
CTTGATTTCCCAACTCGTCT+TGG + Chr6:20872715-20872734 None:intergenic 45.0%
GACCTTGATCTTCTTGTGGA+TGG - Chr6:20873600-20873619 MsG0680031468.01.T01:CDS 45.0%
GGAGAATACAAGAGGTTGGA+AGG - Chr6:20873504-20873523 MsG0680031468.01.T01:CDS 45.0%
GTGGATGGTAGCTATGTTTC+TGG - Chr6:20873615-20873634 MsG0680031468.01.T01:CDS 45.0%
GTGTGCAACTTGCAAAGCAT+GGG - Chr6:20873388-20873407 MsG0680031468.01.T01:intron 45.0%
TACCATGCGTTCACCTTGTT+CGG + Chr6:20872678-20872697 None:intergenic 45.0%
TGCTACGTCACAGTGAATGT+AGG + Chr6:20872768-20872787 None:intergenic 45.0%
! GGCAGATGATGTGTTGTTCT+TGG - Chr6:20873551-20873570 MsG0680031468.01.T01:CDS 45.0%
!!! ATTAACTAATTTTAAATATT+AGG - Chr6:20872848-20872867 MsG0680031468.01.T01:intron 5.0%
AAAATAGCCATCGTCACCGG+AGG - Chr6:20872614-20872633 MsG0680031468.01.T01:CDS 50.0%
CAAGTTGCTGCATCCATTGG+AGG - Chr6:20872731-20872750 MsG0680031468.01.T01:CDS 50.0%
CGGTTAACTTCAAGCCACCA+TGG + Chr6:20873322-20873341 None:intergenic 50.0%
GAAACATGTGGCACGTGCAA+TGG - Chr6:20873244-20873263 MsG0680031468.01.T01:intron 50.0%
GGCAAAATAGCCATCGTCAC+CGG - Chr6:20872611-20872630 MsG0680031468.01.T01:CDS 50.0%
GGCATTGTGCGTGAAACATG+TGG - Chr6:20873232-20873251 MsG0680031468.01.T01:intron 50.0%
GTGCATGTCTTTGCCGAACA+AGG - Chr6:20872662-20872681 MsG0680031468.01.T01:CDS 50.0%
TCCCAACTCGTCTTGGATGT+CGG + Chr6:20872708-20872727 None:intergenic 50.0%
ACATGTGGCACGTGCAATGG+TGG - Chr6:20873247-20873266 MsG0680031468.01.T01:intron 55.0%
CTTCAAGCCACCATGGCTAC+CGG + Chr6:20873315-20873334 None:intergenic 55.0%
GAGGGGTAGCATTGTGTGCA+CGG - Chr6:20873280-20873299 MsG0680031468.01.T01:intron 55.0%
GCCGACATCCAAGACGAGTT+GGG - Chr6:20872704-20872723 MsG0680031468.01.T01:CDS 55.0%
GGTGCATCTATGGCCTCCAA+TGG + Chr6:20872747-20872766 None:intergenic 55.0%
TGCCGAACAAGGTGAACGCA+TGG - Chr6:20872673-20872692 MsG0680031468.01.T01:CDS 55.0%
!! CAGCGGCATTGGCAAAGAGA+CGG - Chr6:20872640-20872659 MsG0680031468.01.T01:CDS 55.0%
CGCCGACATCCAAGACGAGT+TGG - Chr6:20872703-20872722 MsG0680031468.01.T01:CDS 60.0%
CGTCTCTTTGCCAATGCCGC+TGG + Chr6:20872642-20872661 None:intergenic 60.0%
! GTGGCACGTGCAATGGTGGA+CGG - Chr6:20873251-20873270 MsG0680031468.01.T01:intron 60.0%
!! AATGGTGGACGGGTGCGTGA+GGG - Chr6:20873262-20873281 MsG0680031468.01.T01:intron 60.0%
!! TGGCACGTGCAATGGTGGAC+GGG - Chr6:20873252-20873271 MsG0680031468.01.T01:intron 60.0%
!! ATCGTCACCGGAGGTGCCAG+CGG - Chr6:20872623-20872642 MsG0680031468.01.T01:CDS 65.0%
!! ATGGTGGACGGGTGCGTGAG+GGG - Chr6:20873263-20873282 MsG0680031468.01.T01:intron 65.0%
!! CAATGGTGGACGGGTGCGTG+AGG - Chr6:20873261-20873280 MsG0680031468.01.T01:intron 65.0%
AGCGTCGCCGGTAGCCATGG+TGG - Chr6:20873305-20873324 MsG0680031468.01.T01:intron 70.0%
GCCAATGCCGCTGGCACCTC+CGG + Chr6:20872633-20872652 None:intergenic 70.0%
GCTAGCGTCGCCGGTAGCCA+TGG - Chr6:20873302-20873321 MsG0680031468.01.T01:intron 70.0%
GCTGGCACCTCCGGTGACGA+TGG + Chr6:20872624-20872643 None:intergenic 70.0%
GTGTGCACGGCTAGCGTCGC+CGG - Chr6:20873293-20873312 MsG0680031468.01.T01:intron 70.0%
!! ACCGGAGGTGCCAGCGGCAT+TGG - Chr6:20872629-20872648 MsG0680031468.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr6 gene 20872569 20873659 20872569 ID=MsG0680031468.01;Name=MsG0680031468.01
Chr6 mRNA 20872569 20873659 20872569 ID=MsG0680031468.01.T01;Parent=MsG0680031468.01;Name=MsG0680031468.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|3|0|198
Chr6 exon 20873452 20873659 20873452 ID=MsG0680031468.01.T01:exon:13250;Parent=MsG0680031468.01.T01
Chr6 exon 20872914 20873012 20872914 ID=MsG0680031468.01.T01:exon:13249;Parent=MsG0680031468.01.T01
Chr6 exon 20872569 20872858 20872569 ID=MsG0680031468.01.T01:exon:13248;Parent=MsG0680031468.01.T01
Chr6 CDS 20873452 20873659 20873452 ID=MsG0680031468.01.T01:cds;Parent=MsG0680031468.01.T01
Chr6 CDS 20872914 20873012 20872914 ID=MsG0680031468.01.T01:cds;Parent=MsG0680031468.01.T01
Chr6 CDS 20872569 20872858 20872569 ID=MsG0680031468.01.T01:cds;Parent=MsG0680031468.01.T01
Gene Sequence

>MsG0680031468.01.T01

ATGGCAGAAGCTTCATTCACTAACAACAATCTTAGGTTAGCAGGCAAAATAGCCATCGTCACCGGAGGTGCCAGCGGCATTGGCAAAGAGACGGTGCATGTCTTTGCCGAACAAGGTGAACGCATGGTAGTGATCGCCGACATCCAAGACGAGTTGGGAAATCAAGTTGCTGCATCCATTGGAGGCCATAGATGCACCTACATTCACTTTAACATTCGAGGAATGGCATTGTGCGTGAAACATGTGGCACGTGCAATGGTGGACGGGTGCGTGAGGGGTAGCATTGTGTGCACGGCTAGCGTCGCCGGGTTGATGCGTTCGGCTAGTGTGCAACTTGCAAAGCATGGGATAAGGGTGAATTGTGTATCACCAAATGGATTAGCAACACCATTGACATGTAAATTGCTAGATGCAGGTTCAAAGACAGTTGATTTGATTTTTGGAGAATACAAGAGGTTGGAAGGAGTGGTTCTCAACACTAAACATGTGGCAGATGATGTGTTGTTCTTGGTATCTAATGAATCTGACTTTGTCACTGACCTTGATCTTCTTGTGGATGGTAGCTATGTTTCTGGGAAATCTGAATTAATATTTTAA

Protein sequence

>MsG0680031468.01.T01

MAEASFTNNNLRLAGKIAIVTGGASGIGKETVHVFAEQGERMVVIADIQDELGNQVAASIGGHRCTYIHFNIRGMALCVKHVARAMVDGCVRGSIVCTASVAGLMRSASVQLAKHGIRVNCVSPNGLATPLTCKLLDAGSKTVDLIFGEYKRLEGVVLNTKHVADDVLFLVSNESDFVTDLDLLVDGSYVSGKSELIF*