AlfalfaGEDB | MsG0480019461.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019461.01

KEGG: -
GO: -
PFAM: PF05498

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019461.01.T01	XP_003629616.1	86	100	8	2	1	94	20	119	3.47E-51	167
MsG0480019461.01.T02	XP_003629616.1	89.231	65	2	1	1	60	55	119	1.28E-30	113

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019461.01.T01	O23262	42.553	94	41	4	12	94	26	117	6.88E-15	67.4

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019461.01.T01	B7FMN1	86.000	100	8	2	1	94	20	119	1.66e-51	167
MsG0480019461.01.T02	B7FMN1	89.231	65	2	1	1	60	55	119	6.13e-31	113

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480019461.01	MsG0480023013.01	0.813895	2.029983e-51	1.342242e-48
MsG0280007609.01	MsG0480019461.01	0.815192	1.048581e-51	7.180372e-49

PPI

Gene1	Gene2	Type
MsG0480019461.01	MsG0880047048.01	PPI
MsG0480019461.01	MsG0880047048.01	PPI
MsG0880047048.01	MsG0480019461.01	PPI
MsG0880047048.01	MsG0480019461.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019461.01.T01	MTR_8g083150	86.000	100	8	2	1	94	20	119	4.21e-55	167
MsG0480019461.01.T01	MTR_8g083150	85.567	97	8	2	1	91	20	116	3.63e-53	162
MsG0480019461.01.T02	MTR_8g083150	89.231	65	2	1	1	60	55	119	1.55e-34	113
MsG0480019461.01.T02	MTR_8g083150	88.710	62	2	1	1	57	55	116	1.42e-32	108

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019461.01.T01	AT4G14010	42.553	94	41	4	12	94	26	117	7.02e-16	67.4
MsG0480019461.01.T01	AT1G02900	46.154	78	41	1	16	92	43	120	2.06e-11	56.2
MsG0480019461.01.T01	AT4G15800	44.737	76	38	2	20	91	40	115	9.60e-11	54.3
MsG0480019461.01.T02	AT4G14010	46.970	66	27	3	1	60	54	117	3.47e-11	54.3

Find 15 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTCTAAGTATTATCGTTGT+AGG	0.352031	4:+20672186	MsG0480019461.01.T01:CDS
TTAGAACATCCTCCATTATA+AGG	0.354741	4:-20672170	None:intergenic
TCAAGAAGATGAACAATTGA+TGG	0.375070	4:+20672019	MsG0480019461.01.T01:CDS
GCAGGGTGTATTCCTTATAA+TGG	0.377661	4:+20672158	MsG0480019461.01.T01:CDS
TTCTTGATTGCATTCAGCTA+TGG	0.463317	4:-20672003	None:intergenic
TCTGCACCACCATTACAAAC+TGG	0.470502	4:-20672113	None:intergenic
GAAGCTTATAGTAAATCTGC+AGG	0.482571	4:+20672140	MsG0480019461.01.T01:CDS
GATAAACCAGTTTGTAATGG+TGG	0.534773	4:+20672107	MsG0480019461.01.T01:CDS
AGAGATAAACCAGTTTGTAA+TGG	0.541924	4:+20672104	MsG0480019461.01.T01:CDS
CAAAGGAGATACATTTCTGA+AGG	0.595659	4:+20672071	MsG0480019461.01.T01:CDS
GTTTGTAATGGTGGTGCAGA+TGG	0.603398	4:+20672116	MsG0480019461.01.T01:CDS
TGGAGTCTGAAATAAGCAGA+AGG	0.635317	4:+20672039	MsG0480019461.01.T01:CDS
GGGTGTATTCCTTATAATGG+AGG	0.638963	4:+20672161	MsG0480019461.01.T01:CDS
CATCATGCAAGCATATGCAG+TGG	0.670844	4:+20671978	MsG0480019461.01.T01:CDS
AAGCTTATAGTAAATCTGCA+GGG	0.695265	4:+20672141	MsG0480019461.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGCTTATAGTAAATCTGCA+GGG	+	Chr4:20672141-20672160	MsG0480019461.01.T01:CDS	30.0%
	AGAGATAAACCAGTTTGTAA+TGG	+	Chr4:20672104-20672123	MsG0480019461.01.T01:CDS	30.0%
	TCAAGAAGATGAACAATTGA+TGG	+	Chr4:20672019-20672038	MsG0480019461.01.T01:CDS	30.0%
	TTAGAACATCCTCCATTATA+AGG	-	Chr4:20672173-20672192	None:intergenic	30.0%
!	GTTCTAAGTATTATCGTTGT+AGG	+	Chr4:20672186-20672205	MsG0480019461.01.T01:CDS	30.0%
!	TGAAATAAGCAGAAGGTTTT+TGG	+	Chr4:20672046-20672065	MsG0480019461.01.T01:CDS	30.0%
	CAAAGGAGATACATTTCTGA+AGG	+	Chr4:20672071-20672090	MsG0480019461.01.T01:CDS	35.0%
	GAAGCTTATAGTAAATCTGC+AGG	+	Chr4:20672140-20672159	MsG0480019461.01.T01:CDS	35.0%
	GATAAACCAGTTTGTAATGG+TGG	+	Chr4:20672107-20672126	MsG0480019461.01.T01:CDS	35.0%
	TTCTTGATTGCATTCAGCTA+TGG	-	Chr4:20672006-20672025	None:intergenic	35.0%
	GCAGGGTGTATTCCTTATAA+TGG	+	Chr4:20672158-20672177	MsG0480019461.01.T01:CDS	40.0%
	GGGTGTATTCCTTATAATGG+AGG	+	Chr4:20672161-20672180	MsG0480019461.01.T01:CDS	40.0%
!	TGGAGTCTGAAATAAGCAGA+AGG	+	Chr4:20672039-20672058	MsG0480019461.01.T01:CDS	40.0%
	CATCATGCAAGCATATGCAG+TGG	+	Chr4:20671978-20671997	MsG0480019461.01.T01:CDS	45.0%
	TCTGCACCACCATTACAAAC+TGG	-	Chr4:20672116-20672135	None:intergenic	45.0%
!	GTTTGTAATGGTGGTGCAGA+TGG	+	Chr4:20672116-20672135	MsG0480019461.01.T01:CDS	45.0%
!!!	GCAGAAGGTTTTTGGAGCAA+AGG	+	Chr4:20672054-20672073	MsG0480019461.01.T01:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	20671936	20672220	20671936	ID=MsG0480019461.01;Name=MsG0480019461.01
Chr4	mRNA	20671936	20672220	20671936	ID=MsG0480019461.01.T01;Parent=MsG0480019461.01;Name=MsG0480019461.01.T01;_AED=0.34;_eAED=0.34;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|94
Chr4	exon	20671936	20672220	20671936	ID=MsG0480019461.01.T01:exon:10037;Parent=MsG0480019461.01.T01
Chr4	CDS	20671936	20672220	20671936	ID=MsG0480019461.01.T01:cds;Parent=MsG0480019461.01.T01
Chr4	mRNA	20672038	20672220	20672038	ID=MsG0480019461.01.T02;Parent=MsG0480019461.01;Name=MsG0480019461.01.T02;_AED=0.40;_eAED=0.40;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|60
Chr4	exon	20672038	20672220	20672038	ID=MsG0480019461.01.T02:exon:10038;Parent=MsG0480019461.01.T02
Chr4	CDS	20672038	20672220	20672038	ID=MsG0480019461.01.T02:cds;Parent=MsG0480019461.01.T02

Gene Sequence

>MsG0480019461.01.T01

TATACTCATTTTAGCAGCACTGTAATATCATTGAGTCGTAATCATCATGCAAGCATATGCAGTGGTTCCATAGCTGAATGCAATCAAGAAGATGAACAATTGATGGAGTCTGAAATAAGCAGAAGGTTTTTGGAGCAAAGGAGATACATTTCTGAAGGAGCTCTTAAGAGAGATAAACCAGTTTGTAATGGTGGTGCAGATGGTGAAGCTTATAGTAAATCTGCAGGGTGTATTCCTTATAATGGAGGATGTTCTAAGTATTATCGTTGTAGGTCTGACTCTTGA

>MsG0480019461.01.T02

ATGGAGTCTGAAATAAGCAGAAGGTTTTTGGAGCAAAGGAGATACATTTCTGAAGGAGCTCTTAAGAGAGATAAACCAGTTTGTAATGGTGGTGCAGATGGTGAAGCTTATAGTAAATCTGCAGGGTGTATTCCTTATAATGGAGGATGTTCTAAGTATTATCGTTGTAGGTCTGACTCTTGA

Protein sequence

>MsG0480019461.01.T01

YTHFSSTVISLSRNHHASICSGSIAECNQEDEQLMESEISRRFLEQRRYISEGALKRDKPVCNGGADGEAYSKSAGCIPYNGGCSKYYRCRSDS*

>MsG0480019461.01.T02

MESEISRRFLEQRRYISEGALKRDKPVCNGGADGEAYSKSAGCIPYNGGCSKYYRCRSDS*