AlfalfaGEDB | MsG0480023013.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023013.01

KEGG: -
GO: -
PFAM: PF05498

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023013.01.T01	KEH20648.1	83.721	43	7	0	1	43	55	97	1.02E-15	77.4

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023013.01.T01	A0A072TUW6	83.721	43	7	0	1	43	55	97	4.86e-16	77.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047814.01	MsG0480023013.01	0.866189	3.418947e-65	1.142238e-61
MsG0080048058.01	MsG0480023013.01	0.809832	1.557362e-50	9.239747e-48
MsG0180000001.01	MsG0480023013.01	0.835865	1.308469e-56	1.620630e-53
MsG0180000197.01	MsG0480023013.01	0.835815	1.346797e-56	1.665575e-53
MsG0180000446.01	MsG0480023013.01	0.802060	6.711304e-49	3.255996e-46
MsG0180001006.01	MsG0480023013.01	0.804989	1.657394e-49	8.668011e-47
MsG0180001117.01	MsG0480023013.01	0.829896	3.982219e-55	4.130836e-52
MsG0180001237.01	MsG0480023013.01	0.824261	8.882267e-54	7.827411e-51
MsG0180001294.01	MsG0480023013.01	0.855142	7.691170e-62	1.758144e-58
MsG0180003337.01	MsG0480023013.01	0.802637	5.104184e-49	2.512959e-46
MsG0180003409.01	MsG0480023013.01	0.805371	1.378507e-49	7.280568e-47
MsG0180004147.01	MsG0480023013.01	0.802358	5.827701e-49	2.849017e-46
MsG0180004991.01	MsG0480023013.01	0.801085	1.063607e-48	5.034606e-46
MsG0180005125.01	MsG0480023013.01	0.800387	1.476335e-48	6.865365e-46
MsG0180006023.01	MsG0480023013.01	0.803971	2.702628e-49	1.376870e-46
MsG0180006226.01	MsG0480023013.01	-0.803899	2.797044e-49	1.422395e-46
MsG0180006235.01	MsG0480023013.01	0.816225	6.170120e-52	4.346781e-49
MsG0280010633.01	MsG0480023013.01	0.819326	1.231690e-52	9.448116e-50
MsG0280010868.01	MsG0480023013.01	0.815430	9.280922e-52	6.397416e-49
MsG0280011282.01	MsG0480023013.01	0.805341	1.399103e-49	7.383386e-47
MsG0380012471.01	MsG0480023013.01	0.803437	3.488209e-49	1.752844e-46
MsG0480018462.01	MsG0480023013.01	0.807780	4.276532e-50	2.403253e-47
MsG0480018641.01	MsG0480023013.01	0.802782	4.764834e-49	2.354689e-46
MsG0480018846.01	MsG0480023013.01	0.801938	7.109835e-49	3.438617e-46
MsG0480019461.01	MsG0480023013.01	0.813895	2.029983e-51	1.342242e-48
MsG0480020242.01	MsG0480023013.01	0.800974	1.120559e-48	5.289328e-46
MsG0480020591.01	MsG0480023013.01	0.823654	1.232991e-53	1.068251e-50
MsG0480021156.01	MsG0480023013.01	0.801785	7.643434e-49	3.682374e-46
MsG0480021812.01	MsG0480023013.01	0.835249	1.872787e-56	2.277423e-53
MsG0480022167.01	MsG0480023013.01	0.847116	1.417341e-59	2.493568e-56
MsG0480022325.01	MsG0480023013.01	0.800847	1.189340e-48	5.595790e-46
MsG0480022620.01	MsG0480023013.01	0.818737	1.677088e-52	1.265737e-49
MsG0480022717.01	MsG0480023013.01	0.809635	1.716769e-50	1.013336e-47
MsG0480023013.01	MsG0480023105.01	0.846410	2.210570e-59	3.801723e-56
MsG0480023013.01	MsG0480023245.01	0.802964	4.369203e-49	2.169093e-46
MsG0480023013.01	MsG0480023421.01	0.823546	1.306875e-53	1.128653e-50
MsG0480023013.01	MsG0480023447.01	0.805592	1.239373e-49	6.582867e-47
MsG0480023013.01	MsG0480023500.01	0.812676	3.760100e-51	2.405799e-48
MsG0480023013.01	MsG0480023869.01	-0.807427	5.080630e-50	2.829084e-47
MsG0480023013.01	MsG0580024035.01	0.801193	1.010585e-48	4.796506e-46
MsG0480023013.01	MsG0580024478.01	0.814297	1.655358e-51	1.106530e-48
MsG0480023013.01	MsG0580024618.01	0.817368	3.418858e-52	2.485046e-49
MsG0480023013.01	MsG0580024721.01	0.808809	2.581030e-50	1.490090e-47
MsG0480023013.01	MsG0580024793.01	0.802023	6.829243e-49	3.310072e-46
MsG0480023013.01	MsG0580024819.01	0.800442	1.438575e-48	6.699123e-46
MsG0480023013.01	MsG0580025072.01	0.810579	1.074776e-50	6.502888e-48
MsG0480023013.01	MsG0580025352.01	0.802763	4.808179e-49	2.374952e-46
MsG0480023013.01	MsG0580026157.01	0.802562	5.290674e-49	2.599725e-46
MsG0480023013.01	MsG0580028060.01	0.821179	4.633128e-53	3.742458e-50
MsG0480023013.01	MsG0580029148.01	0.825046	5.801741e-54	5.229985e-51
MsG0480023013.01	MsG0580029640.01	0.810740	9.916426e-51	6.026095e-48
MsG0480023013.01	MsG0580030182.01	0.827549	1.471003e-54	1.424659e-51
MsG0480023013.01	MsG0580030235.01	0.821669	3.570348e-53	2.923601e-50
MsG0480023013.01	MsG0680030436.01	0.821723	3.469202e-53	2.845106e-50
MsG0480023013.01	MsG0680031651.01	0.830285	3.200031e-55	3.356574e-52
MsG0480023013.01	MsG0680034061.01	0.808080	3.691467e-50	2.090909e-47
MsG0480023013.01	MsG0780038837.01	0.847472	1.131460e-59	2.013385e-56
MsG0480023013.01	MsG0780038937.01	0.800480	1.413091e-48	6.586599e-46
MsG0480023013.01	MsG0780039137.01	0.817111	3.907013e-52	2.819846e-49
MsG0480023013.01	MsG0780039622.01	-0.802219	6.225100e-49	3.032297e-46
MsG0480023013.01	MsG0780040030.01	0.800820	1.204346e-48	5.662273e-46
MsG0480023013.01	MsG0780040482.01	0.808747	2.660335e-50	1.533402e-47
MsG0480023013.01	MsG0780040861.01	0.818161	2.264127e-52	1.681876e-49
MsG0480023013.01	MsG0780041469.01	0.808341	3.247802e-50	1.852393e-47
MsG0480023013.01	MsG0880041883.01	0.814514	1.482098e-51	9.965691e-49
MsG0480023013.01	MsG0880042543.01	0.811180	7.963056e-51	4.895798e-48
MsG0480023013.01	MsG0880042552.01	0.826199	3.093269e-54	2.881325e-51
MsG0480023013.01	MsG0880042583.01	0.804062	2.586418e-49	1.320828e-46
MsG0480023013.01	MsG0880042811.01	0.815650	8.292382e-52	5.750825e-49
MsG0480023013.01	MsG0880042829.01	0.805997	1.018803e-49	5.468166e-47
MsG0480023013.01	MsG0880044510.01	0.803813	2.914266e-49	1.478686e-46
MsG0480023013.01	MsG0880046027.01	0.801185	1.014219e-48	4.812790e-46
MsG0480023013.01	MsG0880046113.01	0.803881	2.820938e-49	1.433876e-46
MsG0480023013.01	MsG0880046165.01	0.817794	2.740481e-52	2.015418e-49
MsG0480023013.01	MsG0880046762.01	0.846845	1.681588e-59	2.932542e-56
MsG0480023013.01	MsG0880046933.01	0.835787	1.369189e-56	1.691921e-53
MsG0480023013.01	MsG0880047016.01	0.814153	1.781312e-51	1.186021e-48
MsG0480023013.01	MsG0880047192.01	0.822764	1.989297e-53	1.680650e-50
MsG0480023013.01	MsG0880047590.01	0.806653	7.408385e-50	4.043034e-47
MsG0380015793.01	MsG0480023013.01	-0.853528	2.251292e-61	4.879238e-58
MsG0380015831.01	MsG0480023013.01	0.806898	6.577614e-50	3.612544e-47
MsG0380016227.01	MsG0480023013.01	0.809126	2.207188e-50	1.285201e-47
MsG0380016389.01	MsG0480023013.01	0.803346	3.642852e-49	1.826203e-46
MsG0380016483.01	MsG0480023013.01	0.801043	1.084670e-48	5.128958e-46
MsG0380016765.01	MsG0480023013.01	0.800310	1.530094e-48	7.101853e-46
MsG0380016971.01	MsG0480023013.01	0.806831	6.794593e-50	3.725338e-47
MsG0380016985.01	MsG0480023013.01	0.813099	3.037918e-51	1.965527e-48
MsG0380016994.01	MsG0480023013.01	0.808026	3.791035e-50	2.144211e-47
MsG0380017195.01	MsG0480023013.01	0.811069	8.417240e-51	5.159959e-48
MsG0380017404.01	MsG0480023013.01	0.816433	5.544278e-52	3.927879e-49
MsG0380017998.01	MsG0480023013.01	0.808514	2.983597e-50	1.709351e-47
MsG0280007071.01	MsG0480023013.01	0.809476	1.856978e-50	1.091371e-47
MsG0280007089.01	MsG0480023013.01	0.818102	2.335615e-52	1.732095e-49
MsG0280007609.01	MsG0480023013.01	0.861491	9.884101e-64	2.801863e-60
MsG0280007764.01	MsG0480023013.01	0.807044	6.127280e-50	3.377940e-47
MsG0280007790.01	MsG0480023013.01	0.843768	1.143396e-58	1.807517e-55
MsG0280007876.01	MsG0480023013.01	0.841221	5.420474e-58	7.910228e-55
MsG0280008327.01	MsG0480023013.01	0.842690	2.216667e-58	3.387366e-55
MsG0280008853.01	MsG0480023013.01	0.813960	1.964158e-51	1.300945e-48
MsG0280008885.01	MsG0480023013.01	0.815233	1.026832e-51	7.039461e-49
MsG0280009514.01	MsG0480023013.01	0.813781	2.151691e-51	1.418231e-48
MsG0280010002.01	MsG0480023013.01	0.833855	4.194940e-56	4.892311e-53
MsG0280010389.01	MsG0480023013.01	0.829328	5.474617e-55	5.585941e-52

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023013.01.T01	MTR_8g083150	83.721	43	7	0	1	43	55	97	1.23e-19	77.4
MsG0480023013.01.T01	MTR_8g083150	83.721	43	7	0	1	43	55	97	1.28e-19	77.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 13 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACACAGTCATTAAATTTAA+TGG	0.139040	4:-79692389	None:intergenic
TTAGAACATCCTCTATTATA+AGG	0.298490	4:-79692297	None:intergenic
TAAATTTAATGACTGTGTAT+TGG	0.345553	4:+79692393	MsG0480023013.01.T01:CDS
GTTCTAAGTATTATCGTTGT+AGG	0.352031	4:+79692313	MsG0480023013.01.T01:CDS
CTGCACCACCATTACAAAAC+TGG	0.383759	4:-79692225	None:intergenic
CCTCTATTATAAGGATTTGA+AGG	0.425611	4:-79692288	None:intergenic
CCTTCAAATCCTTATAATAG+AGG	0.483889	4:+79692288	MsG0480023013.01.T01:CDS
TGAAGCTTATAGTAATCTGC+AGG	0.496612	4:+79692252	MsG0480023013.01.T01:CDS
TTATAAGGATTTGAAGGTGG+AGG	0.542122	4:-79692282	None:intergenic
CAAAGGAGATACATTTCTGA+AGG	0.595659	4:+79692184	MsG0480023013.01.T01:CDS
CTATTATAAGGATTTGAAGG+TGG	0.614457	4:-79692285	None:intergenic
TGGAGTCAGAAATAAGCAGA+AGG	0.637756	4:+79692152	MsG0480023013.01.T01:CDS
GAAGCTTATAGTAATCTGCA+GGG	0.675948	4:+79692253	MsG0480023013.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAATTTAATGACTGTGTAT+TGG	+	Chr4:79692393-79692412	MsG0480023013.01.T01:CDS	20.0%
!!	TACACAGTCATTAAATTTAA+TGG	-	Chr4:79692392-79692411	None:intergenic	20.0%
!	TTAGAACATCCTCTATTATA+AGG	-	Chr4:79692300-79692319	None:intergenic	25.0%
	CCTCTATTATAAGGATTTGA+AGG	-	Chr4:79692291-79692310	None:intergenic	30.0%
	CCTTCAAATCCTTATAATAG+AGG	+	Chr4:79692288-79692307	MsG0480023013.01.T01:CDS	30.0%
	CTATTATAAGGATTTGAAGG+TGG	-	Chr4:79692288-79692307	None:intergenic	30.0%
!	AGAAATAAGCAGAAGGTTTT+TGG	+	Chr4:79692159-79692178	MsG0480023013.01.T01:CDS	30.0%
!	GTTCTAAGTATTATCGTTGT+AGG	+	Chr4:79692313-79692332	MsG0480023013.01.T01:CDS	30.0%
!!	AGAGATAACCAGTTTTGTAA+TGG	+	Chr4:79692217-79692236	MsG0480023013.01.T01:CDS	30.0%
	CAAAGGAGATACATTTCTGA+AGG	+	Chr4:79692184-79692203	MsG0480023013.01.T01:CDS	35.0%
	GAAGCTTATAGTAATCTGCA+GGG	+	Chr4:79692253-79692272	MsG0480023013.01.T01:CDS	35.0%
	TGAAGCTTATAGTAATCTGC+AGG	+	Chr4:79692252-79692271	MsG0480023013.01.T01:CDS	35.0%
	TTATAAGGATTTGAAGGTGG+AGG	-	Chr4:79692285-79692304	None:intergenic	35.0%
!!	GATAACCAGTTTTGTAATGG+TGG	+	Chr4:79692220-79692239	MsG0480023013.01.T01:CDS	35.0%
	TGGAGTCAGAAATAAGCAGA+AGG	+	Chr4:79692152-79692171	MsG0480023013.01.T01:CDS	40.0%
	CTGCACCACCATTACAAAAC+TGG	-	Chr4:79692228-79692247	None:intergenic	45.0%
!!	CAGTTTTGTAATGGTGGTGC+AGG	+	Chr4:79692226-79692245	MsG0480023013.01.T01:CDS	45.0%
!!	TTTTGTAATGGTGGTGCAGG+TGG	+	Chr4:79692229-79692248	MsG0480023013.01.T01:CDS	45.0%
!!!	GCAGAAGGTTTTTGGAGCAA+AGG	+	Chr4:79692167-79692186	MsG0480023013.01.T01:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	79692151	79692441	79692151	ID=MsG0480023013.01;Name=MsG0480023013.01
Chr4	mRNA	79692151	79692441	79692151	ID=MsG0480023013.01.T01;Parent=MsG0480023013.01;Name=MsG0480023013.01.T01;_AED=0.25;_eAED=0.25;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|96
Chr4	exon	79692151	79692441	79692151	ID=MsG0480023013.01.T01:exon:6156;Parent=MsG0480023013.01.T01
Chr4	CDS	79692151	79692441	79692151	ID=MsG0480023013.01.T01:cds;Parent=MsG0480023013.01.T01

Gene Sequence	>MsG0480023013.01.T01 ATGGAGTCAGAAATAAGCAGAAGGTTTTTGGAGCAAAGGAGATACATTTCTGAAGGAGCTCTTAAAAGAGATAACCAGTTTTGTAATGGTGGTGCAGGTGGTGAAGCTTATAGTAATCTGCAGGGTGTATTCCTCCACCTTCAAATCCTTATAATAGAGGATGTTCTAAGTATTATCGTTGTAGGTCTGATTCTTGAATTAATGAACAAGCTTTTATGCAAACAACTTTTTTTGTTTTCCATTAAATTTAATGACTGTGTATTGGAAATTGTAAGAAATTTTACAAAATAA
Protein sequence	>MsG0480023013.01.T01 MESEISRRFLEQRRYISEGALKRDNQFCNGGAGGEAYSNLQGVFLHLQILIIEDVLSIIVVGLILELMNKLLCKQLFLFSIKFNDCVLEIVRNFTK*