AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019492.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019492.01.T01 MTR_1g115920 84.498 458 29 4 88 504 378 834 0.0 681
MsG0480019492.01.T01 MTR_4g094355 83.654 208 13 2 62 269 65 251 3.15e-105 322
MsG0480019492.01.T01 MTR_4g094355 83.516 182 12 1 341 504 259 440 7.31e-98 303
MsG0480019492.01.T01 MTR_5g039050 74.020 204 24 4 232 415 67 261 1.63e-77 249
MsG0480019492.01.T01 MTR_5g039050 82.222 45 7 1 94 137 6 50 1.39e-14 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019492.01.T01 AT1G28060 60.000 455 138 5 88 501 335 786 2.39e-175 513
MsG0480019492.01.T01 AT1G28060 60.000 455 138 5 88 501 335 786 2.39e-175 513
MsG0480019492.01.T01 AT1G28060 60.000 455 138 5 88 501 335 786 2.39e-175 513
MsG0480019492.01.T01 AT3G55930 51.481 270 89 5 269 498 3 270 3.60e-69 223

Find 130 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACACAAGAACACATTTAT+TGG 0.135860 4:-21222001 None:intergenic
ACAAGTTTGTCCAATGTATT+TGG 0.138799 4:-21221747 None:intergenic
ATAAAATTGAAGAGTAAATT+TGG 0.190886 4:+21221121 MsG0480019492.01.T01:CDS
TGCTGGGGTTCCTCATTATT+GGG 0.233213 4:+21222110 MsG0480019492.01.T01:CDS
ATGCTGGGGTTCCTCATTAT+TGG 0.242299 4:+21222109 MsG0480019492.01.T01:CDS
ACAAACTTGTCTCACTTTAC+AGG 0.244618 4:+21221760 MsG0480019492.01.T01:CDS
CTTCTGCATTCTGGAAATTA+TGG 0.253483 4:+21221304 MsG0480019492.01.T01:CDS
CCCTGTCTCTGCTTCCTTCT+CGG 0.262332 4:-21220443 None:intergenic
AGGAAGCAGAGACAGGGATA+TGG 0.287407 4:+21220449 MsG0480019492.01.T01:CDS
CTTTGCTAGCTGTGCTTGTT+TGG 0.291419 4:-21221170 None:intergenic
CTCGGTCTTCTCTGTGATTC+CGG 0.301582 4:-21220425 None:intergenic
ACTCATCTTGACCTTTGTTT+TGG 0.302345 4:-21221553 None:intergenic
TCCTGTTTAGTTGGCTTGAT+AGG 0.304342 4:-21221448 None:intergenic
TCACCTTAGCTATACGCCTT+TGG 0.310566 4:-21221489 None:intergenic
ATTAAGTTCGGATTGATATC+AGG 0.315138 4:-21221205 None:intergenic
CACCTTAGCTATACGCCTTT+GGG 0.317473 4:-21221488 None:intergenic
AAGCAACCTTAGCACATTAA+AGG 0.318390 4:+21220916 MsG0480019492.01.T01:CDS
TTCTTCTGCTCCTGTTTAGT+TGG 0.318787 4:-21221457 None:intergenic
GACGTGGCACTTCTGCATTC+TGG 0.335270 4:+21221295 MsG0480019492.01.T01:CDS
GTTCATCTATTTCACGTCCC+TGG 0.343803 4:-21220865 None:intergenic
GGTGGAGAGGCGGGCTCAGC+AGG 0.346160 4:-21221418 None:intergenic
TGAAATACTAAAACCTGTAT+TGG 0.355353 4:+21220970 MsG0480019492.01.T01:CDS
GAACCTATTGAAGCTCGATT+TGG 0.361063 4:-21222037 None:intergenic
AGATGAAGGCAATATGAAAA+TGG 0.363556 4:+21221351 MsG0480019492.01.T01:CDS
CATCAGAATCATGCCGGTTC+CGG 0.363965 4:-21220380 None:intergenic
TGCAGCTGCTGAGCGTGAGC+AGG 0.369144 4:+21221644 MsG0480019492.01.T01:CDS
AACGTAACCATCACCGGAAC+CGG 0.388029 4:+21220367 MsG0480019492.01.T01:CDS
TGTTTGTGGAGGAAGGAAAA+TGG 0.392737 4:+21221080 MsG0480019492.01.T01:CDS
TCTGTAATTTCTATTAAGTT+CGG 0.393587 4:-21221217 None:intergenic
AGCAAGTCAATTAAGAGGTA+TGG 0.404160 4:+21221904 MsG0480019492.01.T01:CDS
CAAGTTTGTCCAATGTATTT+GGG 0.405319 4:-21221746 None:intergenic
TTCTTCTCACGCAGCTCAGC+AGG 0.405655 4:-21221702 None:intergenic
TTGCCAAATCGAGCTTCAAT+AGG 0.406342 4:+21222034 MsG0480019492.01.T01:CDS
TCAAAATGAGGATTAGATTC+AGG 0.407433 4:-21221001 None:intergenic
ACCTATCAAGCCAACTAAAC+AGG 0.413749 4:+21221447 MsG0480019492.01.T01:CDS
TTCATCTATTTCACGTCCCT+GGG 0.414957 4:-21220864 None:intergenic
ATAAACTCTAGAGCGGGCCT+TGG 0.420863 4:-21221802 None:intergenic
GGTTACGTTGCGGTCATGTT+CGG 0.424139 4:-21220353 None:intergenic
ACCGAGAAGGAAGCAGAGAC+AGG 0.430015 4:+21220442 MsG0480019492.01.T01:CDS
TTTAATGTGCTAAGGTTGCT+TGG 0.430099 4:-21220914 None:intergenic
CACCACTCAATATCCGGAAT+CGG 0.431295 4:-21221271 None:intergenic
GGAAGCAGAGACAGGGATAT+GGG 0.431480 4:+21220450 MsG0480019492.01.T01:CDS
GGCTCAGCAGGCGGTTCAAT+AGG 0.433119 4:-21221406 None:intergenic
CAAAGCTCCTGCGGCCTAAG+AGG 0.436122 4:+21221047 MsG0480019492.01.T01:CDS
TGTTCTTCGTCTTGACGCCC+AGG 0.441585 4:+21220847 MsG0480019492.01.T01:CDS
AACAGAAAATTCATCCTCTT+AGG 0.449563 4:-21221061 None:intergenic
AAAACTGAAGGACCCGATTC+CGG 0.455391 4:+21221258 MsG0480019492.01.T01:CDS
ACGTCCCACCACTCAATATC+CGG 0.458176 4:-21221277 None:intergenic
TGCTGAGCTGCGTGAGAAGA+AGG 0.474449 4:+21221704 MsG0480019492.01.T01:CDS
CATTTGTATAGTTGACAGCT+TGG 0.476752 4:-21222134 None:intergenic
GGTTGAGGTGGTGGAGAGGC+GGG 0.476862 4:-21221427 None:intergenic
GTGAGCAGGCTCACATAGAT+AGG 0.485650 4:+21221658 MsG0480019492.01.T01:CDS
TTCAGAAGAAAGCACACAGT+TGG 0.492012 4:+21220778 MsG0480019492.01.T01:CDS
ATGTTCGGAGTGTCGCCTAT+CGG 0.492896 4:-21220338 None:intergenic
AATCATGCCGGTTCCGGTGA+TGG 0.495362 4:-21220374 None:intergenic
TGGGCGTCAAGACGAAGAAC+AGG 0.500740 4:-21220845 None:intergenic
AGATCCCACCAGGCTTGAAA+AGG 0.500964 4:+21221611 MsG0480019492.01.T01:intron
TCAGGATCGACATCCAATAC+AGG 0.501479 4:-21220983 None:intergenic
CAAACTTGTCTCACTTTACA+GGG 0.502069 4:+21221761 MsG0480019492.01.T01:CDS
AGGTTGAGGTGGTGGAGAGG+CGG 0.504638 4:-21221428 None:intergenic
GCCTTCATCTATAGTACCGT+TGG 0.504964 4:-21221338 None:intergenic
GGAGCCTTGGTTGGTTTCTG+AGG 0.506975 4:-21220824 None:intergenic
AACACATTTATTGGCATCGT+CGG 0.510492 4:-21221992 None:intergenic
ACCACTCAATATCCGGAATC+GGG 0.511345 4:-21221270 None:intergenic
GCAAGTCAATTAAGAGGTAT+GGG 0.512247 4:+21221905 MsG0480019492.01.T01:CDS
TCAGAAGAAAGCACACAGTT+GGG 0.515196 4:+21220779 MsG0480019492.01.T01:CDS
TAAACTCTAGAGCGGGCCTT+GGG 0.515382 4:-21221801 None:intergenic
CGAAGAACAGGAGCCTTGGT+TGG 0.517067 4:-21220833 None:intergenic
TTATGCTTAGACGTATCAAA+TGG 0.519358 4:+21221932 MsG0480019492.01.T01:CDS
GTGGAAGCAAGTCAATTAAG+AGG 0.522404 4:+21221899 MsG0480019492.01.T01:CDS
GGCTTGATAGGTTGAGGTGG+TGG 0.526396 4:-21221436 None:intergenic
GTTTGATGACCCAAATACAT+TGG 0.529532 4:+21221737 MsG0480019492.01.T01:CDS
ACTTTCATGTGGAACATCCT+CGG 0.530971 4:+21221383 MsG0480019492.01.T01:CDS
GACCGCAACGTAACCATCAC+CGG 0.531946 4:+21220361 MsG0480019492.01.T01:CDS
GAGCCTTGGTTGGTTTCTGA+GGG 0.533893 4:-21220823 None:intergenic
GACCCAAAGGCGTATAGCTA+AGG 0.534462 4:+21221486 MsG0480019492.01.T01:CDS
AGCTCAAGCAAAAGAGCAGA+AGG 0.535132 4:+21221147 MsG0480019492.01.T01:CDS
GGATATTGAGTGGTGGGACG+TGG 0.536150 4:+21221279 MsG0480019492.01.T01:CDS
TCCCAAACCCTAACATGGAC+GGG 0.540259 4:+21220305 None:intergenic
CCCATTCTCTCATCAAAATG+AGG 0.549338 4:-21221013 None:intergenic
CAAACAAGCACAGCTAGCAA+AGG 0.555686 4:+21221171 MsG0480019492.01.T01:CDS
TTCTGAGGGACAGTAACGTC+GGG 0.556866 4:-21220809 None:intergenic
AAGACGAAGAACAGGAGCCT+TGG 0.557281 4:-21220837 None:intergenic
TTCATCTATAGTACCGTTGG+CGG 0.558630 4:-21221335 None:intergenic
GACGAAAGAGAGGGTAGCAA+AGG 0.561832 4:+21220483 MsG0480019492.01.T01:CDS
GGAGAGGCGGGCTCAGCAGG+CGG 0.561936 4:-21221415 None:intergenic
TGTTGACCTTTAATGTGCTA+AGG 0.561959 4:-21220922 None:intergenic
GTTCTTCGTCTTGACGCCCA+GGG 0.562372 4:+21220848 MsG0480019492.01.T01:CDS
AATTCATCCTCTTAGGCCGC+AGG 0.563858 4:-21221054 None:intergenic
TTAGTTGGCTTGATAGGTTG+AGG 0.576255 4:-21221442 None:intergenic
GGAAGAATTCAACTTTCATG+TGG 0.576369 4:+21221372 MsG0480019492.01.T01:CDS
CTCCCAAACCCTAACATGGA+CGG 0.577911 4:+21220304 None:intergenic
GGTCAGATCGATGATGGCGG+TGG 0.582301 4:-21220404 None:intergenic
CCGAGAAGGAAGCAGAGACA+GGG 0.584656 4:+21220443 MsG0480019492.01.T01:CDS
GTTGGCTTGATAGGTTGAGG+TGG 0.586185 4:-21221439 None:intergenic
GCCAACGGTACTATAGATGA+AGG 0.586217 4:+21221337 MsG0480019492.01.T01:CDS
GATTCCGGTCAGATCGATGA+TGG 0.589596 4:-21220410 None:intergenic
ATTAACATAAACTCTAGAGC+GGG 0.591581 4:-21221808 None:intergenic
TGCTTAGACGTATCAAATGG+AGG 0.592616 4:+21221935 MsG0480019492.01.T01:CDS
TATGGTGACATTGCCGCCAA+CGG 0.595136 4:+21221322 MsG0480019492.01.T01:CDS
GACAGGGATATGGGATACGA+TGG 0.607091 4:+21220459 MsG0480019492.01.T01:CDS
TCATAGAACCGCCAAAACAA+AGG 0.609111 4:+21221542 MsG0480019492.01.T01:CDS
ACCGCCATCATCGATCTGAC+CGG 0.610844 4:+21220406 MsG0480019492.01.T01:CDS
GCTTAGACGTATCAAATGGA+GGG 0.611542 4:+21221936 MsG0480019492.01.T01:CDS
CGATTCCGGATATTGAGTGG+TGG 0.614030 4:+21221272 MsG0480019492.01.T01:CDS
AAATGACCTCTCTCATCCCA+AGG 0.616566 4:+21221785 MsG0480019492.01.T01:CDS
TGTAATGAAAGCAAAACTGA+AGG 0.617069 4:+21221246 MsG0480019492.01.T01:CDS
ACAGCTTGGTCCCAATAATG+AGG 0.617442 4:-21222120 None:intergenic
GAATTTGAGTTATTCAACTG+AGG 0.620565 4:-21220758 None:intergenic
ATACGATGGAGACGAAAGAG+AGG 0.628066 4:+21220473 MsG0480019492.01.T01:CDS
GCAGGCGGTTCAATAGGCCG+AGG 0.628346 4:-21221400 None:intergenic
TTCCGGTGATGGTTACGTTG+CGG 0.630451 4:-21220363 None:intergenic
CGACAGGAGATGATTAGACA+TGG 0.631416 4:+21221517 MsG0480019492.01.T01:CDS
GGTGGTCATCAGAATCATGC+CGG 0.631724 4:-21220386 None:intergenic
CCAATAAATGTGTTCTTGTG+TGG 0.635554 4:+21222001 MsG0480019492.01.T01:CDS
AAATGTGTTCTTGTGTGGCA+AGG 0.639668 4:+21222006 MsG0480019492.01.T01:CDS
GATAGGTTGAGGTGGTGGAG+AGG 0.641107 4:-21221431 None:intergenic
TTTCTGAGGGACAGTAACGT+CGG 0.642738 4:-21220810 None:intergenic
GATTCCGGATATTGAGTGGT+GGG 0.645660 4:+21221273 MsG0480019492.01.T01:CDS
ACCCGATTCCGGATATTGAG+TGG 0.647460 4:+21221269 MsG0480019492.01.T01:CDS
AGCGGGCCTTGGGATGAGAG+AGG 0.647591 4:-21221791 None:intergenic
AGAAGAAACTAAAGACCCAA+AGG 0.655642 4:+21221473 MsG0480019492.01.T01:CDS
GGACGTGAAATAGATGAACA+TGG 0.655823 4:+21220869 MsG0480019492.01.T01:CDS
ACGGGAGAGACAAATCCGAT+AGG 0.669354 4:+21220323 MsG0480019492.01.T01:CDS
AATCACAGAGAAGACCGAGA+AGG 0.670001 4:+21220429 MsG0480019492.01.T01:CDS
TACGATGGAGACGAAAGAGA+GGG 0.671943 4:+21220474 MsG0480019492.01.T01:CDS
CATTAACATAAACTCTAGAG+CGG 0.690364 4:-21221809 None:intergenic
TCGACAAAACAAAGCTCCTG+CGG 0.696622 4:+21221038 MsG0480019492.01.T01:CDS
TCCGGTCAGATCGATGATGG+CGG 0.696675 4:-21220407 None:intergenic
TGTCCCTCAGAAACCAACCA+AGG 0.741969 4:+21220820 MsG0480019492.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATTGAAGAGTAAATT+TGG + Chr4:21221121-21221140 MsG0480019492.01.T01:CDS 15.0%
!! TCTGTAATTTCTATTAAGTT+CGG - Chr4:21221220-21221239 None:intergenic 20.0%
! AATTGGATGATAGAGAATTA+GGG + Chr4:21220596-21220615 MsG0480019492.01.T01:intron 25.0%
! CAAGATGAGTAATTTACATA+AGG + Chr4:21221566-21221585 MsG0480019492.01.T01:intron 25.0%
! TGAAATACTAAAACCTGTAT+TGG + Chr4:21220970-21220989 MsG0480019492.01.T01:CDS 25.0%
AACAGAAAATTCATCCTCTT+AGG - Chr4:21221064-21221083 None:intergenic 30.0%
ACAAGTTTGTCCAATGTATT+TGG - Chr4:21221750-21221769 None:intergenic 30.0%
AGATGAAGGCAATATGAAAA+TGG + Chr4:21221351-21221370 MsG0480019492.01.T01:CDS 30.0%
ATGATAGAGAATTAGGGTTA+GGG + Chr4:21220602-21220621 MsG0480019492.01.T01:intron 30.0%
ATTAAGTTCGGATTGATATC+AGG - Chr4:21221208-21221227 None:intergenic 30.0%
CAAGTTTGTCCAATGTATTT+GGG - Chr4:21221749-21221768 None:intergenic 30.0%
GAATTGGATGATAGAGAATT+AGG + Chr4:21220595-21220614 MsG0480019492.01.T01:intron 30.0%
TCAAAATGAGGATTAGATTC+AGG - Chr4:21221004-21221023 None:intergenic 30.0%
TGTAATGAAAGCAAAACTGA+AGG + Chr4:21221246-21221265 MsG0480019492.01.T01:CDS 30.0%
TTATGCTTAGACGTATCAAA+TGG + Chr4:21221932-21221951 MsG0480019492.01.T01:CDS 30.0%
! AGTAATTTACATAAGGTGCT+AGG + Chr4:21221573-21221592 MsG0480019492.01.T01:intron 30.0%
! CATTAACATAAACTCTAGAG+CGG - Chr4:21221812-21221831 None:intergenic 30.0%
! GAATTTGAGTTATTCAACTG+AGG - Chr4:21220761-21220780 None:intergenic 30.0%
! GTAATTTACATAAGGTGCTA+GGG + Chr4:21221574-21221593 MsG0480019492.01.T01:intron 30.0%
!! ATTAACATAAACTCTAGAGC+GGG - Chr4:21221811-21221830 None:intergenic 30.0%
AACACATTTATTGGCATCGT+CGG - Chr4:21221995-21222014 None:intergenic 35.0%
AAGCAACCTTAGCACATTAA+AGG + Chr4:21220916-21220935 MsG0480019492.01.T01:CDS 35.0%
ACAAACTTGTCTCACTTTAC+AGG + Chr4:21221760-21221779 MsG0480019492.01.T01:CDS 35.0%
AGAAGAAACTAAAGACCCAA+AGG + Chr4:21221473-21221492 MsG0480019492.01.T01:CDS 35.0%
AGCAAGTCAATTAAGAGGTA+TGG + Chr4:21221904-21221923 MsG0480019492.01.T01:CDS 35.0%
CAAACTTGTCTCACTTTACA+GGG + Chr4:21221761-21221780 MsG0480019492.01.T01:CDS 35.0%
CATTTGTATAGTTGACAGCT+TGG - Chr4:21222137-21222156 None:intergenic 35.0%
CCAATAAATGTGTTCTTGTG+TGG + Chr4:21222001-21222020 MsG0480019492.01.T01:CDS 35.0%
CCACACAAGAACACATTTAT+TGG - Chr4:21222004-21222023 None:intergenic 35.0%
GATGATAGAGAATTAGGGTT+AGG + Chr4:21220601-21220620 MsG0480019492.01.T01:intron 35.0%
GGAAGAATTCAACTTTCATG+TGG + Chr4:21221372-21221391 MsG0480019492.01.T01:CDS 35.0%
TCTTATCTTTCTCTTATGCG+AGG - Chr4:21220556-21220575 None:intergenic 35.0%
TGTTGACCTTTAATGTGCTA+AGG - Chr4:21220925-21220944 None:intergenic 35.0%
TTTAATGTGCTAAGGTTGCT+TGG - Chr4:21220917-21220936 None:intergenic 35.0%
! AATTTTCTGTTTGTGGAGGA+AGG + Chr4:21221073-21221092 MsG0480019492.01.T01:CDS 35.0%
! CTTCTGCATTCTGGAAATTA+TGG + Chr4:21221304-21221323 MsG0480019492.01.T01:CDS 35.0%
! GAGGATGAATTTTCTGTTTG+TGG + Chr4:21221066-21221085 MsG0480019492.01.T01:CDS 35.0%
! GATGAATTTTCTGTTTGTGG+AGG + Chr4:21221069-21221088 MsG0480019492.01.T01:CDS 35.0%
! GCAAGTCAATTAAGAGGTAT+GGG + Chr4:21221905-21221924 MsG0480019492.01.T01:CDS 35.0%
! GTAAAGTTTTTGTGGATGCT+GGG + Chr4:21222094-21222113 MsG0480019492.01.T01:CDS 35.0%
! GTTTGATGACCCAAATACAT+TGG + Chr4:21221737-21221756 MsG0480019492.01.T01:CDS 35.0%
! TAAAGTTTTTGTGGATGCTG+GGG + Chr4:21222095-21222114 MsG0480019492.01.T01:CDS 35.0%
!! ACTCATCTTGACCTTTGTTT+TGG - Chr4:21221556-21221575 None:intergenic 35.0%
!! AGGAGATTTGGTGATAAACT+TGG + Chr4:21220648-21220667 MsG0480019492.01.T01:intron 35.0%
!! CCTCATTTTGATGAGAGAAT+GGG + Chr4:21221013-21221032 MsG0480019492.01.T01:CDS 35.0%
!! TCCTCATTTTGATGAGAGAA+TGG + Chr4:21221012-21221031 MsG0480019492.01.T01:CDS 35.0%
AAATCGTTTGACTGGATGTG+CGG + Chr4:21221839-21221858 MsG0480019492.01.T01:intron 40.0%
AAATGTGTTCTTGTGTGGCA+AGG + Chr4:21222006-21222025 MsG0480019492.01.T01:CDS 40.0%
AAGAAGGAACGTAGGAGATT+TGG + Chr4:21220636-21220655 MsG0480019492.01.T01:intron 40.0%
ACCTATCAAGCCAACTAAAC+AGG + Chr4:21221447-21221466 MsG0480019492.01.T01:CDS 40.0%
ACTTTCATGTGGAACATCCT+CGG + Chr4:21221383-21221402 MsG0480019492.01.T01:CDS 40.0%
AGAAGAATGTAGCGATGATC+AGG + Chr4:21220573-21220592 MsG0480019492.01.T01:intron 40.0%
ATGTAGCGATGATCAGGAAT+TGG + Chr4:21220579-21220598 MsG0480019492.01.T01:intron 40.0%
CCCATTCTCTCATCAAAATG+AGG - Chr4:21221016-21221035 None:intergenic 40.0%
GAACCTATTGAAGCTCGATT+TGG - Chr4:21222040-21222059 None:intergenic 40.0%
GCTCAACAAAATCGTTTGAC+TGG + Chr4:21221831-21221850 MsG0480019492.01.T01:intron 40.0%
GCTTAGACGTATCAAATGGA+GGG + Chr4:21221936-21221955 MsG0480019492.01.T01:CDS 40.0%
GGACGTGAAATAGATGAACA+TGG + Chr4:21220869-21220888 MsG0480019492.01.T01:CDS 40.0%
GTGGAAGCAAGTCAATTAAG+AGG + Chr4:21221899-21221918 MsG0480019492.01.T01:CDS 40.0%
TAGTAGTGATGCCTTAGCAA+AGG + Chr4:21220680-21220699 MsG0480019492.01.T01:intron 40.0%
TCATAGAACCGCCAAAACAA+AGG + Chr4:21221542-21221561 MsG0480019492.01.T01:CDS 40.0%
TGCTTAGACGTATCAAATGG+AGG + Chr4:21221935-21221954 MsG0480019492.01.T01:CDS 40.0%
TGTTTGTGGAGGAAGGAAAA+TGG + Chr4:21221080-21221099 MsG0480019492.01.T01:CDS 40.0%
TTCATCTATAGTACCGTTGG+CGG - Chr4:21221338-21221357 None:intergenic 40.0%
TTCATCTATTTCACGTCCCT+GGG - Chr4:21220867-21220886 None:intergenic 40.0%
TTCTTCTGCTCCTGTTTAGT+TGG - Chr4:21221460-21221479 None:intergenic 40.0%
TTGCCAAATCGAGCTTCAAT+AGG + Chr4:21222034-21222053 MsG0480019492.01.T01:CDS 40.0%
! AGCTGCTCGTAAAGTTTTTG+TGG + Chr4:21222086-21222105 MsG0480019492.01.T01:CDS 40.0%
! CGTAAAGTTTTTGTGGATGC+TGG + Chr4:21222093-21222112 MsG0480019492.01.T01:CDS 40.0%
! TCAGAAGAAAGCACACAGTT+GGG + Chr4:21220779-21220798 MsG0480019492.01.T01:CDS 40.0%
! TTCAGAAGAAAGCACACAGT+TGG + Chr4:21220778-21220797 MsG0480019492.01.T01:CDS 40.0%
!! TAGGGTTTCTGAAGCAAAGA+AGG + Chr4:21220620-21220639 MsG0480019492.01.T01:intron 40.0%
!! TCCTGTTTAGTTGGCTTGAT+AGG - Chr4:21221451-21221470 None:intergenic 40.0%
!! TTAGTTGGCTTGATAGGTTG+AGG - Chr4:21221445-21221464 None:intergenic 40.0%
!!! CATCTTGACCTTTGTTTTGG+CGG - Chr4:21221553-21221572 None:intergenic 40.0%
AAAACTGAAGGACCCGATTC+CGG + Chr4:21221258-21221277 MsG0480019492.01.T01:CDS 45.0%
AAAGAGAGGGTAGCAAAGGT+AGG + Chr4:21220487-21220506 MsG0480019492.01.T01:intron 45.0%
AAATGACCTCTCTCATCCCA+AGG + Chr4:21221785-21221804 MsG0480019492.01.T01:CDS 45.0%
AATCACAGAGAAGACCGAGA+AGG + Chr4:21220429-21220448 MsG0480019492.01.T01:CDS 45.0%
ACAGCTTGGTCCCAATAATG+AGG - Chr4:21222123-21222142 None:intergenic 45.0%
ACCACTCAATATCCGGAATC+GGG - Chr4:21221273-21221292 None:intergenic 45.0%
AGCTAAGGTGAAAAAGCGAC+AGG + Chr4:21221501-21221520 MsG0480019492.01.T01:CDS 45.0%
AGCTCAAGCAAAAGAGCAGA+AGG + Chr4:21221147-21221166 MsG0480019492.01.T01:CDS 45.0%
ATACGATGGAGACGAAAGAG+AGG + Chr4:21220473-21220492 MsG0480019492.01.T01:CDS 45.0%
ATGCTGGGGTTCCTCATTAT+TGG + Chr4:21222109-21222128 MsG0480019492.01.T01:CDS 45.0%
CAAACAAGCACAGCTAGCAA+AGG + Chr4:21221171-21221190 MsG0480019492.01.T01:CDS 45.0%
CACCACTCAATATCCGGAAT+CGG - Chr4:21221274-21221293 None:intergenic 45.0%
CACCTTAGCTATACGCCTTT+GGG - Chr4:21221491-21221510 None:intergenic 45.0%
CGACAGGAGATGATTAGACA+TGG + Chr4:21221517-21221536 MsG0480019492.01.T01:CDS 45.0%
CTGAAGCAAAGAAGGAACGT+AGG + Chr4:21220628-21220647 MsG0480019492.01.T01:intron 45.0%
CTTTGCTAGCTGTGCTTGTT+TGG - Chr4:21221173-21221192 None:intergenic 45.0%
GATTCCGGATATTGAGTGGT+GGG + Chr4:21221273-21221292 MsG0480019492.01.T01:CDS 45.0%
GCCAACGGTACTATAGATGA+AGG + Chr4:21221337-21221356 MsG0480019492.01.T01:CDS 45.0%
GCCTTCATCTATAGTACCGT+TGG - Chr4:21221341-21221360 None:intergenic 45.0%
GTTAGCGTTGTTGTGGTTGA+AGG + Chr4:21221877-21221896 MsG0480019492.01.T01:intron 45.0%
GTTCATCTATTTCACGTCCC+TGG - Chr4:21220868-21220887 None:intergenic 45.0%
TACGATGGAGACGAAAGAGA+GGG + Chr4:21220474-21220493 MsG0480019492.01.T01:CDS 45.0%
TCACCTTAGCTATACGCCTT+TGG - Chr4:21221492-21221511 None:intergenic 45.0%
TCAGGATCGACATCCAATAC+AGG - Chr4:21220986-21221005 None:intergenic 45.0%
TCGACAAAACAAAGCTCCTG+CGG + Chr4:21221038-21221057 MsG0480019492.01.T01:CDS 45.0%
TGCCTTAGCAAAGGCTAAGA+AGG + Chr4:21220689-21220708 MsG0480019492.01.T01:intron 45.0%
TGCTGGGGTTCCTCATTATT+GGG + Chr4:21222110-21222129 MsG0480019492.01.T01:CDS 45.0%
! AGCCTTCTTAGCCTTTGCTA+AGG - Chr4:21220694-21220713 None:intergenic 45.0%
! CTAAGGCATCACTACTAGAG+AGG - Chr4:21220677-21220696 None:intergenic 45.0%
! GATGTGCGGTGATTTGTGAT+GGG + Chr4:21221853-21221872 MsG0480019492.01.T01:intron 45.0%
! TTTCTGAGGGACAGTAACGT+CGG - Chr4:21220813-21220832 None:intergenic 45.0%
!! ACGTACTTCCTTTTCAAGCC+TGG - Chr4:21221622-21221641 None:intergenic 45.0%
!! ACTTCCTTTTCAAGCCTGGT+GGG - Chr4:21221618-21221637 None:intergenic 45.0%
!! TACTTCCTTTTCAAGCCTGG+TGG - Chr4:21221619-21221638 None:intergenic 45.0%
AACGTAACCATCACCGGAAC+CGG + Chr4:21220367-21220386 MsG0480019492.01.T01:CDS 50.0%
AAGACGAAGAACAGGAGCCT+TGG - Chr4:21220840-21220859 None:intergenic 50.0%
AAGCAACTCAAGATCCCACC+AGG + Chr4:21221601-21221620 MsG0480019492.01.T01:intron 50.0%
AATCGAAGCGCGATGAAGAG+AGG + Chr4:21220514-21220533 MsG0480019492.01.T01:intron 50.0%
AATTCATCCTCTTAGGCCGC+AGG - Chr4:21221057-21221076 None:intergenic 50.0%
ACCCGATTCCGGATATTGAG+TGG + Chr4:21221269-21221288 MsG0480019492.01.T01:CDS 50.0%
ACGGGAGAGACAAATCCGAT+AGG + Chr4:21220323-21220342 MsG0480019492.01.T01:CDS 50.0%
ACGTCCCACCACTCAATATC+CGG - Chr4:21221280-21221299 None:intergenic 50.0%
AGATCCCACCAGGCTTGAAA+AGG + Chr4:21221611-21221630 MsG0480019492.01.T01:intron 50.0%
AGCGTTGTTGTGGTTGAAGG+TGG + Chr4:21221880-21221899 MsG0480019492.01.T01:intron 50.0%
AGGAAGCAGAGACAGGGATA+TGG + Chr4:21220449-21220468 MsG0480019492.01.T01:CDS 50.0%
ATCGAAGCGCGATGAAGAGA+GGG + Chr4:21220515-21220534 MsG0480019492.01.T01:intron 50.0%
CGATTCCGGATATTGAGTGG+TGG + Chr4:21221272-21221291 MsG0480019492.01.T01:CDS 50.0%
CTCGGTCTTCTCTGTGATTC+CGG - Chr4:21220428-21220447 None:intergenic 50.0%
GACAGGGATATGGGATACGA+TGG + Chr4:21220459-21220478 MsG0480019492.01.T01:CDS 50.0%
GACGAAAGAGAGGGTAGCAA+AGG + Chr4:21220483-21220502 MsG0480019492.01.T01:CDS 50.0%
GATGGGTGTTAGCGTTGTTG+TGG + Chr4:21221870-21221889 MsG0480019492.01.T01:intron 50.0%
GATTCCGGTCAGATCGATGA+TGG - Chr4:21220413-21220432 None:intergenic 50.0%
GGAAGCAGAGACAGGGATAT+GGG + Chr4:21220450-21220469 MsG0480019492.01.T01:CDS 50.0%
GGTGGTCATCAGAATCATGC+CGG - Chr4:21220389-21220408 None:intergenic 50.0%
GGTTACGTTGCGGTCATGTT+CGG - Chr4:21220356-21220375 None:intergenic 50.0%
GTGAGCAGGCTCACATAGAT+AGG + Chr4:21221658-21221677 MsG0480019492.01.T01:CDS 50.0%
TATGGTGACATTGCCGCCAA+CGG + Chr4:21221322-21221341 MsG0480019492.01.T01:CDS 50.0%
TGTCCCTCAGAAACCAACCA+AGG + Chr4:21220820-21220839 MsG0480019492.01.T01:CDS 50.0%
! GACCCAAAGGCGTATAGCTA+AGG + Chr4:21221486-21221505 MsG0480019492.01.T01:CDS 50.0%
! GGATGTGCGGTGATTTGTGA+TGG + Chr4:21221852-21221871 MsG0480019492.01.T01:intron 50.0%
! TTCCGGTGATGGTTACGTTG+CGG - Chr4:21220366-21220385 None:intergenic 50.0%
! TTCTGAGGGACAGTAACGTC+GGG - Chr4:21220812-21220831 None:intergenic 50.0%
!! ATAAACTCTAGAGCGGGCCT+TGG - Chr4:21221805-21221824 None:intergenic 50.0%
!! ATGTTCGGAGTGTCGCCTAT+CGG - Chr4:21220341-21220360 None:intergenic 50.0%
!! CATCAGAATCATGCCGGTTC+CGG - Chr4:21220383-21220402 None:intergenic 50.0%
!! GAGCCTTGGTTGGTTTCTGA+GGG - Chr4:21220826-21220845 None:intergenic 50.0%
!! GTTGGCTTGATAGGTTGAGG+TGG - Chr4:21221442-21221461 None:intergenic 50.0%
!! TAAACTCTAGAGCGGGCCTT+GGG - Chr4:21221804-21221823 None:intergenic 50.0%
ACCGAGAAGGAAGCAGAGAC+AGG + Chr4:21220442-21220461 MsG0480019492.01.T01:CDS 55.0%
ACCGCCATCATCGATCTGAC+CGG + Chr4:21220406-21220425 MsG0480019492.01.T01:CDS 55.0%
CCCTGTCTCTGCTTCCTTCT+CGG - Chr4:21220446-21220465 None:intergenic 55.0%
CCGAGAAGGAAGCAGAGACA+GGG + Chr4:21220443-21220462 MsG0480019492.01.T01:CDS 55.0%
CGAAGAACAGGAGCCTTGGT+TGG - Chr4:21220836-21220855 None:intergenic 55.0%
GACCGCAACGTAACCATCAC+CGG + Chr4:21220361-21220380 MsG0480019492.01.T01:CDS 55.0%
GACGTGGCACTTCTGCATTC+TGG + Chr4:21221295-21221314 MsG0480019492.01.T01:CDS 55.0%
GTTCTTCGTCTTGACGCCCA+GGG + Chr4:21220848-21220867 MsG0480019492.01.T01:CDS 55.0%
TGCTGAGCTGCGTGAGAAGA+AGG + Chr4:21221704-21221723 MsG0480019492.01.T01:CDS 55.0%
TGGGCGTCAAGACGAAGAAC+AGG - Chr4:21220848-21220867 None:intergenic 55.0%
TGTTCTTCGTCTTGACGCCC+AGG + Chr4:21220847-21220866 MsG0480019492.01.T01:CDS 55.0%
TTCTTCTCACGCAGCTCAGC+AGG - Chr4:21221705-21221724 None:intergenic 55.0%
! GGATATTGAGTGGTGGGACG+TGG + Chr4:21221279-21221298 MsG0480019492.01.T01:CDS 55.0%
! GGCTTGATAGGTTGAGGTGG+TGG - Chr4:21221439-21221458 None:intergenic 55.0%
! TCCGGTCAGATCGATGATGG+CGG - Chr4:21220410-21220429 None:intergenic 55.0%
!! AATCATGCCGGTTCCGGTGA+TGG - Chr4:21220377-21220396 None:intergenic 55.0%
!! GATAGGTTGAGGTGGTGGAG+AGG - Chr4:21221434-21221453 None:intergenic 55.0%
!! GGAGCCTTGGTTGGTTTCTG+AGG - Chr4:21220827-21220846 None:intergenic 55.0%
CAAAGCTCCTGCGGCCTAAG+AGG + Chr4:21221047-21221066 MsG0480019492.01.T01:CDS 60.0%
GAAGCGCGATGAAGAGAGGG+AGG + Chr4:21220518-21220537 MsG0480019492.01.T01:intron 60.0%
GGCTCAGCAGGCGGTTCAAT+AGG - Chr4:21221409-21221428 None:intergenic 60.0%
!! AGGTTGAGGTGGTGGAGAGG+CGG - Chr4:21221431-21221450 None:intergenic 60.0%
!! GGTCAGATCGATGATGGCGG+TGG - Chr4:21220407-21220426 None:intergenic 60.0%
AGCGGGCCTTGGGATGAGAG+AGG - Chr4:21221794-21221813 None:intergenic 65.0%
GCAGGCGGTTCAATAGGCCG+AGG - Chr4:21221403-21221422 None:intergenic 65.0%
TGCAGCTGCTGAGCGTGAGC+AGG + Chr4:21221644-21221663 MsG0480019492.01.T01:CDS 65.0%
!! GGTTGAGGTGGTGGAGAGGC+GGG - Chr4:21221430-21221449 None:intergenic 65.0%
! GGAGAGGCGGGCTCAGCAGG+CGG - Chr4:21221418-21221437 None:intergenic 75.0%
! GGTGGAGAGGCGGGCTCAGC+AGG - Chr4:21221421-21221440 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 21220319 21222170 21220319 ID=MsG0480019492.01;Name=MsG0480019492.01
Chr4 mRNA 21220319 21222170 21220319 ID=MsG0480019492.01.T01;Parent=MsG0480019492.01;Name=MsG0480019492.01.T01;_AED=0.26;_eAED=0.33;_QI=0|0|0|1|1|1|4|0|504
Chr4 exon 21220319 21220504 21220319 ID=MsG0480019492.01.T01:exon:8089;Parent=MsG0480019492.01.T01
Chr4 exon 21220728 21221563 21220728 ID=MsG0480019492.01.T01:exon:8090;Parent=MsG0480019492.01.T01
Chr4 exon 21221623 21221843 21221623 ID=MsG0480019492.01.T01:exon:8091;Parent=MsG0480019492.01.T01
Chr4 exon 21221899 21222170 21221899 ID=MsG0480019492.01.T01:exon:8092;Parent=MsG0480019492.01.T01
Chr4 CDS 21220319 21220504 21220319 ID=MsG0480019492.01.T01:cds;Parent=MsG0480019492.01.T01
Chr4 CDS 21220728 21221563 21220728 ID=MsG0480019492.01.T01:cds;Parent=MsG0480019492.01.T01
Chr4 CDS 21221623 21221843 21221623 ID=MsG0480019492.01.T01:cds;Parent=MsG0480019492.01.T01
Chr4 CDS 21221899 21222170 21221899 ID=MsG0480019492.01.T01:cds;Parent=MsG0480019492.01.T01
Gene Sequence

>MsG0480019492.01.T01

ATGGACGGGAGAGACAAATCCGATAGGCGACACTCCGAACATGACCGCAACGTAACCATCACCGGAACCGGCATGATTCTGATGACCACCGCCATCATCGATCTGACCGGAATCACAGAGAAGACCGAGAAGGAAGCAGAGACAGGGATATGGGATACGATGGAGACGAAAGAGAGGGTAGCAAAGAAATTGTTGTCAGAGAAGCTGAAGAAAATTCCTCAGTTGAATAACTCAAATTCAGAAGAAAGCACACAGTTGGGTGCTACACCCGACGTTACTGTCCCTCAGAAACCAACCAAGGCTCCTGTTCTTCGTCTTGACGCCCAGGGACGTGAAATAGATGAACATGGAAATGTTGTTAATGTAACTAAGCCAAGCAACCTTAGCACATTAAAGGTCAACATTAATAAACACAAGAGAGATGCATTTGAAATACTAAAACCTGTATTGGATGTCGATCCTGAATCTAATCCTCATTTTGATGAGAGAATGGGTATCGACAAAACAAAGCTCCTGCGGCCTAAGAGGATGAATTTTCTGTTTGTGGAGGAAGGAAAATGGTCAAGAGACGCTGAATCAATAAAATTGAAGAGTAAATTTGGAGAAGCTCAAGCAAAAGAGCAGAAGGCCAAACAAGCACAGCTAGCAAAGGCAAATGCTTCTCCTGATATCAATCCGAACTTAATAGAAATTACAGAGAGAGTTGTAATGAAAGCAAAACTGAAGGACCCGATTCCGGATATTGAGTGGTGGGACGTGGCACTTCTGCATTCTGGAAATTATGGTGACATTGCCGCCAACGGTACTATAGATGAAGGCAATATGAAAATGGAAGAATTCAACTTTCATGTGGAACATCCTCGGCCTATTGAACCGCCTGCTGAGCCCGCCTCTCCACCACCTCAACCTATCAAGCCAACTAAACAGGAGCAGAAGAAACTAAAGACCCAAAGGCGTATAGCTAAGGTGAAAAAGCGACAGGAGATGATTAGACATGGTGTCATAGAACCGCCAAAACAAAGGCTTGAAAAGGAAGTACGTCATGCAGCTGCTGAGCGTGAGCAGGCTCACATAGATAGGAATATTGCACGCAAGCTTACTCCTGCTGAGCTGCGTGAGAAGAAGGAAAGAAAACTGTTTGATGACCCAAATACATTGGACAAACTTGTCTCACTTTACAGGGTAAATGACCTCTCTCATCCCAAGGCCCGCTCTAGAGTTTATGTTAATGCTCAACAAAATCGTGGAAGCAAGTCAATTAAGAGGTATGGGAAAGTTATGCTTAGACGTATCAAATGGAGGGATGTTTCAAAAGAGAAAGAAGATAATGAAGATTCCGACGATGCCAATAAATGTGTTCTTGTGTGGCAAGGAAGTGTTGCCAAATCGAGCTTCAATAGGTTCAGTGTTCATGACTGCATCACTGAAGCAGCTGCTCGTAAAGTTTTTGTGGATGCTGGGGTTCCTCATTATTGGGACCAAGCTGTCAACTATACAAATGATGATGCTGTTTGA
Protein sequence

>MsG0480019492.01.T01

MDGRDKSDRRHSEHDRNVTITGTGMILMTTAIIDLTGITEKTEKEAETGIWDTMETKERVAKKLLSEKLKKIPQLNNSNSEESTQLGATPDVTVPQKPTKAPVLRLDAQGREIDEHGNVVNVTKPSNLSTLKVNINKHKRDAFEILKPVLDVDPESNPHFDERMGIDKTKLLRPKRMNFLFVEEGKWSRDAESIKLKSKFGEAQAKEQKAKQAQLAKANASPDINPNLIEITERVVMKAKLKDPIPDIEWWDVALLHSGNYGDIAANGTIDEGNMKMEEFNFHVEHPRPIEPPAEPASPPPQPIKPTKQEQKKLKTQRRIAKVKKRQEMIRHGVIEPPKQRLEKEVRHAAAEREQAHIDRNIARKLTPAELREKKERKLFDDPNTLDKLVSLYRVNDLSHPKARSRVYVNAQQNRGSKSIKRYGKVMLRRIKWRDVSKEKEDNEDSDDANKCVLVWQGSVAKSSFNRFSVHDCITEAAARKVFVDAGVPHYWDQAVNYTNDDAV*