Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019527.01.T01 | XP_013455259.2 | 94.49 | 363 | 20 | 0 | 1 | 363 | 1 | 363 | 0 | 686 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019527.01.T01 | O24305 | 75.207 | 363 | 87 | 2 | 1 | 363 | 1 | 360 | 0 | 568 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019527.01.T01 | Q06YR0 | 94.490 | 363 | 20 | 0 | 1 | 363 | 1 | 363 | 0.0 | 686 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480019509.01 | MsG0480019527.01 | 0.834212 | 3.414820e-56 | 4.024856e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019527.01.T01 | MTR_4g033200 | 94.490 | 363 | 20 | 0 | 1 | 363 | 1 | 363 | 0.0 | 685 |
| MsG0480019527.01.T01 | MTR_4g033015 | 94.215 | 363 | 21 | 0 | 1 | 363 | 1 | 363 | 0.0 | 683 |
| MsG0480019527.01.T01 | MTR_4g033150 | 90.083 | 363 | 36 | 0 | 1 | 363 | 1 | 363 | 0.0 | 663 |
| MsG0480019527.01.T01 | MTR_4g088200 | 73.554 | 363 | 94 | 2 | 1 | 363 | 1 | 361 | 0.0 | 561 |
| MsG0480019527.01.T01 | MTR_4g088190 | 75.549 | 364 | 88 | 1 | 1 | 363 | 1 | 364 | 0.0 | 543 |
| MsG0480019527.01.T01 | MTR_4g033170 | 76.033 | 363 | 81 | 1 | 1 | 363 | 1 | 357 | 0.0 | 538 |
| MsG0480019527.01.T01 | MTR_4g033085 | 75.758 | 363 | 82 | 1 | 1 | 363 | 1 | 357 | 0.0 | 515 |
| MsG0480019527.01.T01 | MTR_0794s0020 | 90.361 | 249 | 24 | 0 | 1 | 249 | 1 | 249 | 2.67e-170 | 474 |
| MsG0480019527.01.T01 | MTR_7g014570 | 50.964 | 363 | 167 | 4 | 1 | 363 | 1 | 352 | 8.35e-126 | 365 |
| MsG0480019527.01.T01 | MTR_5g074680 | 51.240 | 363 | 167 | 6 | 4 | 362 | 3 | 359 | 2.68e-125 | 365 |
| MsG0480019527.01.T01 | MTR_5g074600 | 50.704 | 355 | 167 | 6 | 11 | 363 | 11 | 359 | 3.46e-124 | 362 |
| MsG0480019527.01.T01 | MTR_5g074580 | 50.141 | 355 | 169 | 5 | 11 | 363 | 12 | 360 | 6.55e-122 | 356 |
| MsG0480019527.01.T01 | MTR_4g088345 | 52.260 | 354 | 160 | 4 | 11 | 363 | 11 | 356 | 9.54e-121 | 353 |
| MsG0480019527.01.T01 | MTR_7g014510 | 47.383 | 363 | 185 | 4 | 1 | 363 | 1 | 357 | 1.43e-118 | 347 |
| MsG0480019527.01.T01 | MTR_1g076940 | 48.361 | 366 | 181 | 6 | 1 | 363 | 1 | 361 | 4.02e-117 | 344 |
| MsG0480019527.01.T01 | MTR_5g074610 | 49.292 | 353 | 146 | 3 | 11 | 363 | 7 | 326 | 8.13e-117 | 342 |
| MsG0480019527.01.T01 | MTR_7g024500 | 46.089 | 358 | 181 | 4 | 4 | 361 | 3 | 348 | 9.22e-114 | 335 |
| MsG0480019527.01.T01 | MTR_7g024520 | 47.207 | 358 | 177 | 4 | 4 | 361 | 3 | 348 | 3.67e-111 | 328 |
| MsG0480019527.01.T01 | MTR_4g079160 | 46.328 | 354 | 181 | 3 | 11 | 363 | 10 | 355 | 9.97e-109 | 322 |
| MsG0480019527.01.T01 | MTR_5g074540 | 46.197 | 355 | 165 | 6 | 11 | 363 | 11 | 341 | 6.90e-108 | 320 |
| MsG0480019527.01.T01 | MTR_4g088340 | 49.853 | 341 | 162 | 4 | 24 | 363 | 1 | 333 | 8.47e-108 | 319 |
| MsG0480019527.01.T01 | MTR_3g083620 | 37.901 | 343 | 198 | 10 | 20 | 359 | 27 | 357 | 4.77e-64 | 208 |
| MsG0480019527.01.T01 | MTR_4g038440 | 34.795 | 365 | 224 | 8 | 3 | 363 | 14 | 368 | 5.00e-60 | 198 |
| MsG0480019527.01.T01 | MTR_1g076930 | 44.033 | 243 | 127 | 5 | 24 | 263 | 1 | 237 | 7.08e-60 | 193 |
| MsG0480019527.01.T01 | MTR_0017s0100 | 35.068 | 365 | 223 | 8 | 3 | 363 | 14 | 368 | 9.19e-59 | 194 |
| MsG0480019527.01.T01 | MTR_0079s0070 | 35.484 | 341 | 206 | 8 | 27 | 363 | 4 | 334 | 2.05e-56 | 187 |
| MsG0480019527.01.T01 | MTR_7g012090 | 32.258 | 341 | 210 | 10 | 32 | 361 | 40 | 370 | 1.34e-45 | 160 |
| MsG0480019527.01.T01 | MTR_7g012070 | 31.672 | 341 | 212 | 11 | 32 | 361 | 40 | 370 | 1.52e-44 | 157 |
| MsG0480019527.01.T01 | MTR_7g011900 | 31.361 | 338 | 217 | 9 | 32 | 362 | 40 | 369 | 2.33e-44 | 157 |
| MsG0480019527.01.T01 | MTR_7g011990 | 30.321 | 343 | 211 | 10 | 32 | 362 | 40 | 366 | 4.09e-44 | 156 |
| MsG0480019527.01.T01 | MTR_8g068870 | 32.033 | 359 | 228 | 10 | 5 | 355 | 6 | 356 | 2.24e-43 | 154 |
| MsG0480019527.01.T01 | MTR_1g036490 | 31.124 | 347 | 223 | 8 | 25 | 362 | 30 | 369 | 1.05e-42 | 152 |
| MsG0480019527.01.T01 | MTR_1g096310 | 29.745 | 353 | 230 | 8 | 4 | 349 | 16 | 357 | 3.05e-42 | 151 |
| MsG0480019527.01.T01 | MTR_1g036460 | 30.931 | 333 | 216 | 7 | 25 | 350 | 30 | 355 | 6.13e-42 | 150 |
| MsG0480019527.01.T01 | MTR_7g012040 | 31.138 | 334 | 217 | 8 | 32 | 361 | 39 | 363 | 1.97e-41 | 149 |
| MsG0480019527.01.T01 | MTR_7g012020 | 31.642 | 335 | 215 | 8 | 32 | 361 | 39 | 364 | 2.48e-40 | 146 |
| MsG0480019527.01.T01 | MTR_2g055940 | 30.448 | 335 | 218 | 9 | 32 | 361 | 34 | 358 | 3.83e-40 | 145 |
| MsG0480019527.01.T01 | MTR_5g098170 | 32.733 | 333 | 205 | 11 | 31 | 355 | 33 | 354 | 4.34e-40 | 145 |
| MsG0480019527.01.T01 | MTR_7g118320 | 31.214 | 346 | 220 | 9 | 9 | 348 | 14 | 347 | 3.13e-39 | 143 |
| MsG0480019527.01.T01 | MTR_8g068690 | 32.000 | 325 | 208 | 8 | 26 | 344 | 40 | 357 | 1.79e-38 | 141 |
| MsG0480019527.01.T01 | MTR_7g118300 | 30.931 | 333 | 214 | 8 | 26 | 353 | 42 | 363 | 2.86e-38 | 140 |
| MsG0480019527.01.T01 | MTR_8g068750 | 31.157 | 337 | 218 | 9 | 26 | 355 | 41 | 370 | 3.07e-38 | 140 |
| MsG0480019527.01.T01 | MTR_0591s0010 | 31.385 | 325 | 210 | 8 | 26 | 344 | 41 | 358 | 4.83e-38 | 140 |
| MsG0480019527.01.T01 | MTR_2g072790 | 31.454 | 337 | 212 | 8 | 31 | 360 | 27 | 351 | 1.25e-37 | 139 |
| MsG0480019527.01.T01 | MTR_3g092900 | 31.173 | 324 | 210 | 8 | 31 | 349 | 34 | 349 | 3.18e-36 | 135 |
| MsG0480019527.01.T01 | MTR_1g036510 | 29.429 | 333 | 221 | 7 | 25 | 350 | 44 | 369 | 4.36e-36 | 135 |
| MsG0480019527.01.T01 | MTR_3g021440 | 31.818 | 330 | 207 | 9 | 28 | 350 | 38 | 356 | 7.50e-36 | 134 |
| MsG0480019527.01.T01 | MTR_8g024160 | 34.400 | 250 | 155 | 5 | 101 | 348 | 71 | 313 | 5.59e-34 | 128 |
| MsG0480019527.01.T01 | MTR_3g096050 | 29.885 | 348 | 222 | 11 | 23 | 362 | 24 | 357 | 4.56e-33 | 126 |
| MsG0480019527.01.T01 | MTR_4g038440 | 32.197 | 264 | 169 | 5 | 3 | 263 | 14 | 270 | 8.20e-33 | 124 |
| MsG0480019527.01.T01 | MTR_3g021430 | 30.395 | 329 | 205 | 9 | 32 | 350 | 29 | 343 | 5.53e-32 | 123 |
| MsG0480019527.01.T01 | MTR_8g068570 | 29.582 | 311 | 179 | 9 | 26 | 330 | 41 | 317 | 2.95e-31 | 120 |
| MsG0480019527.01.T01 | MTR_8g068730 | 29.882 | 338 | 189 | 10 | 26 | 355 | 41 | 338 | 4.34e-31 | 120 |
| MsG0480019527.01.T01 | MTR_6g078850 | 27.874 | 348 | 231 | 9 | 9 | 348 | 33 | 368 | 4.99e-30 | 120 |
| MsG0480019527.01.T01 | MTR_3g096070 | 27.616 | 344 | 208 | 10 | 23 | 360 | 24 | 332 | 1.97e-27 | 110 |
| MsG0480019527.01.T01 | MTR_7g012020 | 29.876 | 241 | 160 | 6 | 32 | 267 | 39 | 275 | 1.58e-25 | 104 |
| MsG0480019527.01.T01 | MTR_1g050220 | 33.766 | 154 | 89 | 2 | 194 | 347 | 147 | 287 | 2.78e-21 | 93.2 |
| MsG0480019527.01.T01 | MTR_8g068740 | 28.000 | 275 | 153 | 8 | 26 | 292 | 41 | 278 | 6.75e-19 | 85.9 |
| MsG0480019527.01.T01 | MTR_3g096050 | 30.364 | 247 | 155 | 9 | 23 | 263 | 24 | 259 | 2.16e-18 | 84.3 |
| MsG0480019527.01.T01 | MTR_8g068690 | 27.236 | 246 | 170 | 6 | 26 | 266 | 40 | 281 | 2.53e-17 | 81.6 |
| MsG0480019527.01.T01 | MTR_1g036510 | 27.642 | 246 | 167 | 6 | 25 | 263 | 44 | 285 | 5.32e-17 | 80.9 |
| MsG0480019527.01.T01 | MTR_7g012020 | 29.524 | 210 | 139 | 6 | 32 | 236 | 39 | 244 | 9.49e-17 | 79.3 |
| MsG0480019527.01.T01 | MTR_8g068600 | 27.155 | 232 | 160 | 6 | 38 | 264 | 27 | 254 | 6.78e-16 | 77.4 |
| MsG0480019527.01.T01 | MTR_4g025570 | 43.750 | 96 | 50 | 3 | 265 | 359 | 122 | 214 | 1.53e-14 | 72.4 |
| MsG0480019527.01.T01 | MTR_4g025570 | 44.304 | 79 | 38 | 2 | 6 | 84 | 16 | 88 | 6.67e-14 | 70.5 |
| MsG0480019527.01.T01 | MTR_8g068620 | 37.363 | 91 | 57 | 0 | 173 | 263 | 20 | 110 | 9.59e-14 | 67.4 |
| MsG0480019527.01.T01 | MTR_8g068760 | 41.053 | 95 | 51 | 3 | 263 | 355 | 135 | 226 | 8.89e-13 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019527.01.T01 | AT4G35160 | 32.877 | 365 | 214 | 12 | 16 | 362 | 29 | 380 | 1.54e-46 | 163 |
| MsG0480019527.01.T01 | AT4G35150 | 29.888 | 358 | 191 | 10 | 16 | 362 | 15 | 323 | 1.43e-45 | 159 |
| MsG0480019527.01.T01 | AT5G54160 | 32.508 | 323 | 207 | 7 | 31 | 350 | 34 | 348 | 2.58e-42 | 151 |
| MsG0480019527.01.T01 | AT1G51990 | 31.871 | 342 | 211 | 11 | 33 | 363 | 32 | 362 | 1.72e-39 | 144 |
| MsG0480019527.01.T01 | AT1G51990 | 32.174 | 345 | 206 | 12 | 33 | 363 | 32 | 362 | 1.32e-38 | 141 |
| MsG0480019527.01.T01 | AT1G77520 | 30.857 | 350 | 211 | 10 | 31 | 362 | 44 | 380 | 1.35e-38 | 142 |
| MsG0480019527.01.T01 | AT3G53140 | 31.579 | 342 | 210 | 11 | 31 | 360 | 27 | 356 | 1.50e-38 | 141 |
| MsG0480019527.01.T01 | AT1G33030 | 29.518 | 332 | 215 | 10 | 31 | 353 | 21 | 342 | 4.15e-35 | 132 |
| MsG0480019527.01.T01 | AT5G53810 | 30.259 | 347 | 222 | 9 | 29 | 362 | 38 | 377 | 1.41e-33 | 128 |
| MsG0480019527.01.T01 | AT1G77530 | 31.977 | 344 | 215 | 9 | 31 | 362 | 44 | 380 | 6.56e-32 | 124 |
| MsG0480019527.01.T01 | AT1G63140 | 28.649 | 370 | 240 | 11 | 9 | 362 | 19 | 380 | 7.19e-32 | 124 |
| MsG0480019527.01.T01 | AT1G63140 | 28.649 | 370 | 240 | 11 | 9 | 362 | 39 | 400 | 7.27e-32 | 124 |
| MsG0480019527.01.T01 | AT1G76790 | 27.419 | 372 | 220 | 16 | 6 | 355 | 8 | 351 | 2.73e-26 | 108 |
| MsG0480019527.01.T01 | AT1G21130 | 25.893 | 336 | 218 | 11 | 31 | 350 | 40 | 360 | 6.56e-23 | 99.0 |
| MsG0480019527.01.T01 | AT1G62900 | 33.333 | 192 | 123 | 3 | 173 | 362 | 16 | 204 | 1.46e-22 | 94.7 |
| MsG0480019527.01.T01 | AT1G21100 | 25.893 | 336 | 218 | 11 | 31 | 350 | 40 | 360 | 3.76e-22 | 96.7 |
| MsG0480019527.01.T01 | AT1G21110 | 25.378 | 331 | 216 | 11 | 31 | 345 | 40 | 355 | 9.01e-22 | 95.9 |
| MsG0480019527.01.T01 | AT5G37170 | 29.897 | 194 | 130 | 4 | 172 | 362 | 43 | 233 | 6.81e-21 | 90.9 |
| MsG0480019527.01.T01 | AT5G37170 | 29.897 | 194 | 130 | 4 | 172 | 362 | 143 | 333 | 9.21e-21 | 92.4 |
| MsG0480019527.01.T01 | AT1G21120 | 25.000 | 336 | 221 | 11 | 31 | 350 | 40 | 360 | 1.19e-20 | 92.4 |
| MsG0480019527.01.T01 | AT1G21120 | 25.000 | 336 | 221 | 11 | 31 | 350 | 88 | 408 | 1.81e-20 | 92.4 |
| MsG0480019527.01.T01 | AT5G37170 | 32.571 | 175 | 109 | 5 | 194 | 362 | 14 | 185 | 5.01e-20 | 87.0 |
| MsG0480019527.01.T01 | AT1G33030 | 26.360 | 239 | 163 | 7 | 31 | 263 | 21 | 252 | 2.94e-14 | 72.4 |
| MsG0480019527.01.T01 | AT1G63140 | 26.022 | 269 | 180 | 8 | 9 | 263 | 39 | 302 | 5.67e-14 | 72.4 |
| MsG0480019527.01.T01 | AT1G33030 | 26.360 | 239 | 163 | 7 | 31 | 263 | 21 | 252 | 6.32e-14 | 71.6 |
Find 68 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGCAAGAGGAGAGTCATTT+TGG | 0.126492 | 4:+21690550 | MsG0480019527.01.T01:CDS |
| GCAGTTCTCCAATATCTTTA+AGG | 0.188779 | 4:-21691292 | None:intergenic |
| ATGATGATGACTTTGTTAAA+TGG | 0.247172 | 4:+21691429 | MsG0480019527.01.T01:CDS |
| TGAATTATCCTTAAAGATAT+TGG | 0.263747 | 4:+21691284 | MsG0480019527.01.T01:CDS |
| TAAAACAAAGGAGTTGAAAT+TGG | 0.284468 | 4:+21691395 | MsG0480019527.01.T01:CDS |
| TGTATATATGTTTGTATAGA+TGG | 0.289789 | 4:-21690149 | None:intergenic |
| CTCCTCTTGCGCTCTCAAAA+AGG | 0.323697 | 4:-21690539 | None:intergenic |
| TTCAGTGAGCCATTTCTTAG+AGG | 0.326776 | 4:-21690502 | None:intergenic |
| TACAGTGGATTCTGCATGAT+TGG | 0.329305 | 4:+21691256 | MsG0480019527.01.T01:intron |
| TAAAGGACACTAACTTTAGA+AGG | 0.352110 | 4:-21690288 | None:intergenic |
| CTTAACAAAGATTCTGAATC+TGG | 0.352374 | 4:+21690579 | MsG0480019527.01.T01:CDS |
| TAGCCATGTGTTTGAAGGTT+TGG | 0.381600 | 4:+21690671 | MsG0480019527.01.T01:CDS |
| AAGAAAGAGAGAAGAAAGAA+TGG | 0.382447 | 4:+21691454 | MsG0480019527.01.T01:CDS |
| GTTGATGTTGGAGGTGGGAG+AGG | 0.383792 | 4:+21690702 | MsG0480019527.01.T01:CDS |
| GCTAATTCAGTAAGAGTCAT+TGG | 0.387374 | 4:-21690246 | None:intergenic |
| CCACTTATGAGAAGCATAGA+AGG | 0.388574 | 4:-21690411 | None:intergenic |
| GATGAAAATTTGAAATTTGT+TGG | 0.393974 | 4:+21690813 | MsG0480019527.01.T01:CDS |
| GTTTGAAGGTTTGGAGTCTC+TGG | 0.426025 | 4:+21690680 | MsG0480019527.01.T01:CDS |
| TGAGGTCAGGAAAAGCTTCA+TGG | 0.428682 | 4:-21690743 | None:intergenic |
| CAAACACATGGCTACACTCT+TGG | 0.432380 | 4:-21690662 | None:intergenic |
| GAGTGTAGCCATGTGTTTGA+AGG | 0.433859 | 4:+21690666 | MsG0480019527.01.T01:CDS |
| GAGTATGTTTCAAGAGGCCT+TGG | 0.441053 | 4:+21690608 | MsG0480019527.01.T01:CDS |
| GATGTTGGAGGTGGGAGAGG+AGG | 0.449491 | 4:+21690705 | MsG0480019527.01.T01:CDS |
| TCTCTGGTTGATGTTGGAGG+TGG | 0.463565 | 4:+21690696 | MsG0480019527.01.T01:CDS |
| CCTTCTATGCTTCTCATAAG+TGG | 0.466077 | 4:+21690411 | MsG0480019527.01.T01:CDS |
| AATACTGTGCATTTGAGGTC+AGG | 0.467274 | 4:-21690756 | None:intergenic |
| TGTGGCACTCCTCTAAGAAA+TGG | 0.479752 | 4:+21690493 | MsG0480019527.01.T01:CDS |
| GGTCAAATACTGTGCATTTG+AGG | 0.486795 | 4:-21690761 | None:intergenic |
| AGAAAGAGAGAAGAAAGAAT+GGG | 0.494469 | 4:+21691455 | MsG0480019527.01.T01:CDS |
| TTGGAGTCTCTGGTTGATGT+TGG | 0.495110 | 4:+21690690 | MsG0480019527.01.T01:CDS |
| ATCAGCTATGCCTAGTTCTA+CGG | 0.496591 | 4:-21690202 | None:intergenic |
| GCAATCGCAAGAGACGATAA+AGG | 0.501675 | 4:-21690305 | None:intergenic |
| CAGGTTGTAAGTAACTTGTC+TGG | 0.502887 | 4:+21690789 | MsG0480019527.01.T01:CDS |
| GAGTCTCTGGTTGATGTTGG+AGG | 0.503583 | 4:+21690693 | MsG0480019527.01.T01:CDS |
| CTCAAATCAGCCGTAGAACT+AGG | 0.506388 | 4:+21690192 | MsG0480019527.01.T01:CDS |
| TGAAGCGATCCGCTAACAAA+TGG | 0.526003 | 4:-21690453 | None:intergenic |
| ATAGACTTGAAGCCACATAT+AGG | 0.526187 | 4:-21691522 | None:intergenic |
| CTCTGGTTGATGTTGGAGGT+GGG | 0.527669 | 4:+21690697 | MsG0480019527.01.T01:CDS |
| CTTCTATGCTTCTCATAAGT+GGG | 0.530784 | 4:+21690412 | MsG0480019527.01.T01:CDS |
| GAAAATTTGAAATTTGTTGG+TGG | 0.531638 | 4:+21690816 | MsG0480019527.01.T01:CDS |
| TGTTTGTCACCATTTGTTAG+CGG | 0.533547 | 4:+21690444 | MsG0480019527.01.T01:CDS |
| GGCTACACTCTTGGAGTGCA+AGG | 0.538863 | 4:-21690653 | None:intergenic |
| ATCCATGTCGGCTGAACATG+TGG | 0.544800 | 4:+21690475 | MsG0480019527.01.T01:CDS |
| TTGAGTAAAACAACATCTGC+AGG | 0.545132 | 4:-21690861 | None:intergenic |
| TGCCACATGTTCAGCCGACA+TGG | 0.546045 | 4:-21690477 | None:intergenic |
| CATCTGCAGGAGGAATAGAT+TGG | 0.556296 | 4:-21690848 | None:intergenic |
| TACAAGATTACACCTATATG+TGG | 0.556787 | 4:+21691510 | MsG0480019527.01.T01:CDS |
| TAAATGGATTTCAGTAGCAA+TGG | 0.570025 | 4:+21690093 | None:intergenic |
| GACAAGTTACTTACAACCTG+TGG | 0.570330 | 4:-21690786 | None:intergenic |
| ATACAGCTTTATAAATTCGA+TGG | 0.577327 | 4:+21690167 | MsG0480019527.01.T01:CDS |
| CACAGTATTTGACCAACCAC+AGG | 0.579462 | 4:+21690770 | MsG0480019527.01.T01:CDS |
| GATCTGAGAATCAGCAGCCA+AGG | 0.594205 | 4:-21690625 | None:intergenic |
| TGAATTGAGTATGTTTCAAG+AGG | 0.602192 | 4:+21690602 | MsG0480019527.01.T01:CDS |
| TGTATAGATGGATTTGAGCA+TGG | 0.604592 | 4:-21690137 | None:intergenic |
| TTGCGATTGCTAACACACAA+TGG | 0.607452 | 4:+21690318 | MsG0480019527.01.T01:CDS |
| GGACACTAACTTTAGAAGGG+TGG | 0.612811 | 4:-21690284 | None:intergenic |
| AGGGTGGAGTTTCAAAGATG+AGG | 0.627418 | 4:-21690268 | None:intergenic |
| AGTTACTTACAACCTGTGGT+TGG | 0.628024 | 4:-21690782 | None:intergenic |
| GCGGATCGCTTCATCCATGT+CGG | 0.632994 | 4:+21690463 | MsG0480019527.01.T01:CDS |
| AAAGGACACTAACTTTAGAA+GGG | 0.634971 | 4:-21690287 | None:intergenic |
| ACACTGATGAATGGCAACGA+AGG | 0.635528 | 4:+21690357 | MsG0480019527.01.T01:CDS |
| GAAATGGCTCACTGAAGACA+AGG | 0.641674 | 4:+21690509 | MsG0480019527.01.T01:CDS |
| GCTGATGCAATCCACAACCA+TGG | 0.643784 | 4:+21690219 | MsG0480019527.01.T01:CDS |
| AAGTGACAATCATAAAACAA+AGG | 0.662807 | 4:+21691383 | MsG0480019527.01.T01:CDS |
| AGTAAAACAACATCTGCAGG+AGG | 0.666155 | 4:-21690858 | None:intergenic |
| GAGAAACTCATATATGACGC+AGG | 0.669510 | 4:+21691477 | MsG0480019527.01.T01:CDS |
| ACTCCAAACCTTCAAACACA+TGG | 0.677292 | 4:-21690674 | None:intergenic |
| CTTATGAGAAGCATAGAAGG+AGG | 0.680160 | 4:-21690408 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTAAAAGATATATTTTTTT+TGG | + | Chr4:21690906-21690925 | MsG0480019527.01.T01:intron | 10.0% |
| !!! | ATAAAATCATTTTTTTACAG+TGG | + | Chr4:21691241-21691260 | MsG0480019527.01.T01:intron | 15.0% |
| !! | TAATTAGTCATCAACAAAAT+TGG | - | Chr4:21691133-21691152 | None:intergenic | 20.0% |
| !! | TGAATTATCCTTAAAGATAT+TGG | + | Chr4:21691284-21691303 | MsG0480019527.01.T01:CDS | 20.0% |
| !! | TGTATATATGTTTGTATAGA+TGG | - | Chr4:21690152-21690171 | None:intergenic | 20.0% |
| !!! | GAGTAATTTTCTTATGTTAT+TGG | + | Chr4:21691093-21691112 | MsG0480019527.01.T01:intron | 20.0% |
| !!! | GATGAAAATTTGAAATTTGT+TGG | + | Chr4:21690813-21690832 | MsG0480019527.01.T01:CDS | 20.0% |
| !!! | TATCTTTTACTCATAACATT+TGG | - | Chr4:21690897-21690916 | None:intergenic | 20.0% |
| ! | AAGTGACAATCATAAAACAA+AGG | + | Chr4:21691383-21691402 | MsG0480019527.01.T01:CDS | 25.0% |
| ! | TAAAACAAAGGAGTTGAAAT+TGG | + | Chr4:21691395-21691414 | MsG0480019527.01.T01:CDS | 25.0% |
| !! | AAAAAAGCTATTTCAGGAAA+AGG | + | Chr4:21691315-21691334 | MsG0480019527.01.T01:CDS | 25.0% |
| !! | AAAAAGCTATTTCAGGAAAA+GGG | + | Chr4:21691316-21691335 | MsG0480019527.01.T01:CDS | 25.0% |
| !! | ATACAGCTTTATAAATTCGA+TGG | + | Chr4:21690167-21690186 | MsG0480019527.01.T01:CDS | 25.0% |
| !! | ATGATGATGACTTTGTTAAA+TGG | + | Chr4:21691429-21691448 | MsG0480019527.01.T01:CDS | 25.0% |
| !!! | GAAAATTTGAAATTTGTTGG+TGG | + | Chr4:21690816-21690835 | MsG0480019527.01.T01:CDS | 25.0% |
| !!! | TGTTGATGACTAATTAATCT+TGG | + | Chr4:21691137-21691156 | MsG0480019527.01.T01:intron | 25.0% |
| !!! | TTAAATGTTAAAAGTGTCAG+CGG | - | Chr4:21690992-21691011 | None:intergenic | 25.0% |
| AAGAAAGAGAGAAGAAAGAA+TGG | + | Chr4:21691454-21691473 | MsG0480019527.01.T01:CDS | 30.0% | |
| AGAAAGAGAGAAGAAAGAAT+GGG | + | Chr4:21691455-21691474 | MsG0480019527.01.T01:CDS | 30.0% | |
| ATGTCGCTGATATAAAAGAA+TGG | - | Chr4:21690956-21690975 | None:intergenic | 30.0% | |
| ATTTCAGGAAAAGGGAAAAA+AGG | + | Chr4:21691324-21691343 | MsG0480019527.01.T01:CDS | 30.0% | |
| CAGAACTGTCACTAATATAA+GGG | + | Chr4:21691046-21691065 | MsG0480019527.01.T01:intron | 30.0% | |
| CTTAACAAAGATTCTGAATC+TGG | + | Chr4:21690579-21690598 | MsG0480019527.01.T01:CDS | 30.0% | |
| TAAAGGACACTAACTTTAGA+AGG | - | Chr4:21690291-21690310 | None:intergenic | 30.0% | |
| TACAAGATTACACCTATATG+TGG | + | Chr4:21691510-21691529 | MsG0480019527.01.T01:CDS | 30.0% | |
| TGAATTGAGTATGTTTCAAG+AGG | + | Chr4:21690602-21690621 | MsG0480019527.01.T01:CDS | 30.0% | |
| TTAGAAATTTCGTAACTGAG+AGG | + | Chr4:21691071-21691090 | MsG0480019527.01.T01:intron | 30.0% | |
| ! | AAAGGACACTAACTTTAGAA+GGG | - | Chr4:21690290-21690309 | None:intergenic | 30.0% |
| ! | AACTGCAAAAAAGCTATTTC+AGG | + | Chr4:21691309-21691328 | MsG0480019527.01.T01:CDS | 30.0% |
| ! | TTCTTTTATATCAGCGACAT+CGG | + | Chr4:21690956-21690975 | MsG0480019527.01.T01:intron | 30.0% |
| AGGAAAAGGGAAAAAAGGAA+AGG | + | Chr4:21691329-21691348 | MsG0480019527.01.T01:CDS | 35.0% | |
| ATAGACTTGAAGCCACATAT+AGG | - | Chr4:21691525-21691544 | None:intergenic | 35.0% | |
| CTTCTATGCTTCTCATAAGT+GGG | + | Chr4:21690412-21690431 | MsG0480019527.01.T01:CDS | 35.0% | |
| GCAAAAACAACACTGATGAA+TGG | + | Chr4:21690348-21690367 | MsG0480019527.01.T01:CDS | 35.0% | |
| GCAGAACTGTCACTAATATA+AGG | + | Chr4:21691045-21691064 | MsG0480019527.01.T01:intron | 35.0% | |
| GCAGTTCTCCAATATCTTTA+AGG | - | Chr4:21691295-21691314 | None:intergenic | 35.0% | |
| GCTAATTCAGTAAGAGTCAT+TGG | - | Chr4:21690249-21690268 | None:intergenic | 35.0% | |
| TTGAGTAAAACAACATCTGC+AGG | - | Chr4:21690864-21690883 | None:intergenic | 35.0% | |
| ! | TGTATAGATGGATTTGAGCA+TGG | - | Chr4:21690140-21690159 | None:intergenic | 35.0% |
| ! | TGTTTGTCACCATTTGTTAG+CGG | + | Chr4:21690444-21690463 | MsG0480019527.01.T01:CDS | 35.0% |
| !!! | TAAGAGTCATTGGTTTTCCA+TGG | - | Chr4:21690239-21690258 | None:intergenic | 35.0% |
| !!! | TGCAGATGTTGTTTTACTCA+AGG | + | Chr4:21690863-21690882 | MsG0480019527.01.T01:CDS | 35.0% |
| ACTCCAAACCTTCAAACACA+TGG | - | Chr4:21690677-21690696 | None:intergenic | 40.0% | |
| AGTAAAACAACATCTGCAGG+AGG | - | Chr4:21690861-21690880 | None:intergenic | 40.0% | |
| AGTTACTTACAACCTGTGGT+TGG | - | Chr4:21690785-21690804 | None:intergenic | 40.0% | |
| CCTTCTATGCTTCTCATAAG+TGG | + | Chr4:21690411-21690430 | MsG0480019527.01.T01:CDS | 40.0% | |
| GACAAGTTACTTACAACCTG+TGG | - | Chr4:21690789-21690808 | None:intergenic | 40.0% | |
| GAGAAACTCATATATGACGC+AGG | + | Chr4:21691477-21691496 | MsG0480019527.01.T01:CDS | 40.0% | |
| GGTCAAATACTGTGCATTTG+AGG | - | Chr4:21690764-21690783 | None:intergenic | 40.0% | |
| TAGCCATGTGTTTGAAGGTT+TGG | + | Chr4:21690671-21690690 | MsG0480019527.01.T01:CDS | 40.0% | |
| TTCAGTGAGCCATTTCTTAG+AGG | - | Chr4:21690505-21690524 | None:intergenic | 40.0% | |
| TTGCGATTGCTAACACACAA+TGG | + | Chr4:21690318-21690337 | MsG0480019527.01.T01:CDS | 40.0% | |
| ! | AATACTGTGCATTTGAGGTC+AGG | - | Chr4:21690759-21690778 | None:intergenic | 40.0% |
| ! | CAGGTTGTAAGTAACTTGTC+TGG | + | Chr4:21690789-21690808 | MsG0480019527.01.T01:CDS | 40.0% |
| ! | CCACTTATGAGAAGCATAGA+AGG | - | Chr4:21690414-21690433 | None:intergenic | 40.0% |
| ! | CTTATGAGAAGCATAGAAGG+AGG | - | Chr4:21690411-21690430 | None:intergenic | 40.0% |
| ! | TACAGTGGATTCTGCATGAT+TGG | + | Chr4:21691256-21691275 | MsG0480019527.01.T01:intron | 40.0% |
| !! | ACCGATCTTAAAAGTGTCAG+CGG | + | Chr4:21691023-21691042 | MsG0480019527.01.T01:intron | 40.0% |
| !! | ATCAGCTATGCCTAGTTCTA+CGG | - | Chr4:21690205-21690224 | None:intergenic | 40.0% |
| !!! | TCATTGGTTTTCCATGGTTG+TGG | - | Chr4:21690233-21690252 | None:intergenic | 40.0% |
| ACACTGATGAATGGCAACGA+AGG | + | Chr4:21690357-21690376 | MsG0480019527.01.T01:CDS | 45.0% | |
| AGGGTGGAGTTTCAAAGATG+AGG | - | Chr4:21690271-21690290 | None:intergenic | 45.0% | |
| CAAACACATGGCTACACTCT+TGG | - | Chr4:21690665-21690684 | None:intergenic | 45.0% | |
| CACAGTATTTGACCAACCAC+AGG | + | Chr4:21690770-21690789 | MsG0480019527.01.T01:CDS | 45.0% | |
| CATCTGCAGGAGGAATAGAT+TGG | - | Chr4:21690851-21690870 | None:intergenic | 45.0% | |
| CTCAAATCAGCCGTAGAACT+AGG | + | Chr4:21690192-21690211 | MsG0480019527.01.T01:CDS | 45.0% | |
| GAAATGGCTCACTGAAGACA+AGG | + | Chr4:21690509-21690528 | MsG0480019527.01.T01:CDS | 45.0% | |
| GAGTATGTTTCAAGAGGCCT+TGG | + | Chr4:21690608-21690627 | MsG0480019527.01.T01:CDS | 45.0% | |
| GAGTGTAGCCATGTGTTTGA+AGG | + | Chr4:21690666-21690685 | MsG0480019527.01.T01:CDS | 45.0% | |
| GCAATCGCAAGAGACGATAA+AGG | - | Chr4:21690308-21690327 | None:intergenic | 45.0% | |
| TGAAGCGATCCGCTAACAAA+TGG | - | Chr4:21690456-21690475 | None:intergenic | 45.0% | |
| TGAGGTCAGGAAAAGCTTCA+TGG | - | Chr4:21690746-21690765 | None:intergenic | 45.0% | |
| ! | GGACACTAACTTTAGAAGGG+TGG | - | Chr4:21690287-21690306 | None:intergenic | 45.0% |
| ! | GTTTGAAGGTTTGGAGTCTC+TGG | + | Chr4:21690680-21690699 | MsG0480019527.01.T01:CDS | 45.0% |
| ! | TGTGGCACTCCTCTAAGAAA+TGG | + | Chr4:21690493-21690512 | MsG0480019527.01.T01:CDS | 45.0% |
| ! | TTGGAGTCTCTGGTTGATGT+TGG | + | Chr4:21690690-21690709 | MsG0480019527.01.T01:CDS | 45.0% |
| !! | CGCAAGAGGAGAGTCATTTT+GGG | + | Chr4:21690551-21690570 | MsG0480019527.01.T01:CDS | 45.0% |
| !!! | GCCGCTGACACTTTTAAGAT+CGG | - | Chr4:21691027-21691046 | None:intergenic | 45.0% |
| CTCCTCTTGCGCTCTCAAAA+AGG | - | Chr4:21690542-21690561 | None:intergenic | 50.0% | |
| GATCTGAGAATCAGCAGCCA+AGG | - | Chr4:21690628-21690647 | None:intergenic | 50.0% | |
| GCTGATGCAATCCACAACCA+TGG | + | Chr4:21690219-21690238 | MsG0480019527.01.T01:CDS | 50.0% | |
| ! | ATCCATGTCGGCTGAACATG+TGG | + | Chr4:21690475-21690494 | MsG0480019527.01.T01:CDS | 50.0% |
| ! | CTCTGGTTGATGTTGGAGGT+GGG | + | Chr4:21690697-21690716 | MsG0480019527.01.T01:CDS | 50.0% |
| ! | GAGTCTCTGGTTGATGTTGG+AGG | + | Chr4:21690693-21690712 | MsG0480019527.01.T01:CDS | 50.0% |
| ! | GCGCAAGAGGAGAGTCATTT+TGG | + | Chr4:21690550-21690569 | MsG0480019527.01.T01:CDS | 50.0% |
| ! | TCTCTGGTTGATGTTGGAGG+TGG | + | Chr4:21690696-21690715 | MsG0480019527.01.T01:CDS | 50.0% |
| !! | AGCCTTTTTGAGAGCGCAAG+AGG | + | Chr4:21690537-21690556 | MsG0480019527.01.T01:CDS | 50.0% |
| GCGGATCGCTTCATCCATGT+CGG | + | Chr4:21690463-21690482 | MsG0480019527.01.T01:CDS | 55.0% | |
| GGCTACACTCTTGGAGTGCA+AGG | - | Chr4:21690656-21690675 | None:intergenic | 55.0% | |
| GTTGATGTTGGAGGTGGGAG+AGG | + | Chr4:21690702-21690721 | MsG0480019527.01.T01:CDS | 55.0% | |
| TGCCACATGTTCAGCCGACA+TGG | - | Chr4:21690480-21690499 | None:intergenic | 55.0% | |
| GATGTTGGAGGTGGGAGAGG+AGG | + | Chr4:21690705-21690724 | MsG0480019527.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 21690096 | 21691563 | 21690096 | ID=MsG0480019527.01;Name=MsG0480019527.01 |
| Chr4 | mRNA | 21690096 | 21691563 | 21690096 | ID=MsG0480019527.01.T01;Parent=MsG0480019527.01;Name=MsG0480019527.01.T01;_AED=0.21;_eAED=0.21;_QI=0|0|0|1|1|1|2|0|363 |
| Chr4 | exon | 21690096 | 21690884 | 21690096 | ID=MsG0480019527.01.T01:exon:8250;Parent=MsG0480019527.01.T01 |
| Chr4 | exon | 21691261 | 21691563 | 21691261 | ID=MsG0480019527.01.T01:exon:8251;Parent=MsG0480019527.01.T01 |
| Chr4 | CDS | 21690096 | 21690884 | 21690096 | ID=MsG0480019527.01.T01:cds;Parent=MsG0480019527.01.T01 |
| Chr4 | CDS | 21691261 | 21691563 | 21691261 | ID=MsG0480019527.01.T01:cds;Parent=MsG0480019527.01.T01 |
| Gene Sequence |
| Protein sequence |
