Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019509.01.T01 | XP_013455259.2 | 93.664 | 363 | 23 | 0 | 1 | 363 | 1 | 363 | 0 | 681 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019509.01.T01 | O24305 | 75.207 | 363 | 87 | 2 | 1 | 363 | 1 | 360 | 0 | 570 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019509.01.T01 | Q06YR0 | 93.664 | 363 | 23 | 0 | 1 | 363 | 1 | 363 | 0.0 | 681 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480019106.01 | MsG0480019509.01 | 0.803061 | 4.171726e-49 | 2.076130e-46 |
| MsG0480019509.01 | MsG0480019527.01 | 0.834212 | 3.414820e-56 | 4.024856e-53 |
| MsG0480019509.01 | MsG0480019533.01 | 0.810351 | 1.203575e-50 | 7.238290e-48 |
| MsG0480019509.01 | MsG0680031386.01 | 0.810659 | 1.032691e-50 | 6.261833e-48 |
| MsG0480019509.01 | MsG0680031875.01 | 0.813649 | 2.299644e-51 | 1.510409e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019509.01.T01 | MTR_4g033200 | 93.388 | 363 | 24 | 0 | 1 | 363 | 1 | 363 | 0.0 | 679 |
| MsG0480019509.01.T01 | MTR_4g033015 | 93.113 | 363 | 25 | 0 | 1 | 363 | 1 | 363 | 0.0 | 676 |
| MsG0480019509.01.T01 | MTR_4g033150 | 89.256 | 363 | 39 | 0 | 1 | 363 | 1 | 363 | 0.0 | 655 |
| MsG0480019509.01.T01 | MTR_4g088200 | 73.278 | 363 | 95 | 1 | 1 | 363 | 1 | 361 | 0.0 | 562 |
| MsG0480019509.01.T01 | MTR_4g033170 | 77.410 | 363 | 76 | 1 | 1 | 363 | 1 | 357 | 0.0 | 552 |
| MsG0480019509.01.T01 | MTR_4g088190 | 74.725 | 364 | 91 | 1 | 1 | 363 | 1 | 364 | 0.0 | 538 |
| MsG0480019509.01.T01 | MTR_4g033085 | 76.584 | 363 | 79 | 1 | 1 | 363 | 1 | 357 | 0.0 | 526 |
| MsG0480019509.01.T01 | MTR_0794s0020 | 88.353 | 249 | 29 | 0 | 1 | 249 | 1 | 249 | 7.56e-166 | 463 |
| MsG0480019509.01.T01 | MTR_7g014570 | 52.066 | 363 | 163 | 4 | 1 | 363 | 1 | 352 | 1.78e-131 | 380 |
| MsG0480019509.01.T01 | MTR_5g074680 | 51.515 | 363 | 166 | 6 | 4 | 362 | 3 | 359 | 2.29e-127 | 370 |
| MsG0480019509.01.T01 | MTR_5g074600 | 50.141 | 355 | 169 | 5 | 11 | 363 | 11 | 359 | 1.93e-125 | 365 |
| MsG0480019509.01.T01 | MTR_4g088345 | 51.977 | 354 | 161 | 4 | 11 | 363 | 11 | 356 | 1.02e-122 | 358 |
| MsG0480019509.01.T01 | MTR_7g014510 | 47.383 | 363 | 185 | 4 | 1 | 363 | 1 | 357 | 1.14e-122 | 358 |
| MsG0480019509.01.T01 | MTR_5g074580 | 50.141 | 355 | 169 | 5 | 11 | 363 | 12 | 360 | 3.19e-122 | 357 |
| MsG0480019509.01.T01 | MTR_1g076940 | 48.907 | 366 | 179 | 6 | 1 | 363 | 1 | 361 | 2.46e-119 | 350 |
| MsG0480019509.01.T01 | MTR_7g024500 | 48.045 | 358 | 174 | 4 | 4 | 361 | 3 | 348 | 5.53e-119 | 348 |
| MsG0480019509.01.T01 | MTR_5g074610 | 49.292 | 353 | 146 | 3 | 11 | 363 | 7 | 326 | 5.76e-118 | 345 |
| MsG0480019509.01.T01 | MTR_7g024520 | 48.603 | 358 | 172 | 4 | 4 | 361 | 3 | 348 | 1.25e-115 | 340 |
| MsG0480019509.01.T01 | MTR_4g088340 | 50.440 | 341 | 160 | 4 | 24 | 363 | 1 | 333 | 6.60e-112 | 330 |
| MsG0480019509.01.T01 | MTR_4g079160 | 46.610 | 354 | 180 | 3 | 11 | 363 | 10 | 355 | 3.88e-111 | 328 |
| MsG0480019509.01.T01 | MTR_5g074540 | 46.479 | 355 | 164 | 6 | 11 | 363 | 11 | 341 | 3.30e-110 | 325 |
| MsG0480019509.01.T01 | MTR_3g083620 | 37.609 | 343 | 199 | 9 | 20 | 359 | 27 | 357 | 4.77e-64 | 208 |
| MsG0480019509.01.T01 | MTR_1g076930 | 45.492 | 244 | 122 | 6 | 24 | 263 | 1 | 237 | 2.92e-62 | 199 |
| MsG0480019509.01.T01 | MTR_4g038440 | 34.795 | 365 | 224 | 8 | 3 | 363 | 14 | 368 | 1.45e-60 | 199 |
| MsG0480019509.01.T01 | MTR_0017s0100 | 34.795 | 365 | 224 | 8 | 3 | 363 | 14 | 368 | 2.50e-59 | 196 |
| MsG0480019509.01.T01 | MTR_0079s0070 | 35.380 | 342 | 207 | 8 | 26 | 363 | 3 | 334 | 4.85e-57 | 189 |
| MsG0480019509.01.T01 | MTR_8g068870 | 33.526 | 346 | 214 | 10 | 18 | 355 | 19 | 356 | 1.78e-48 | 167 |
| MsG0480019509.01.T01 | MTR_7g012090 | 32.845 | 341 | 208 | 10 | 32 | 361 | 40 | 370 | 2.04e-46 | 162 |
| MsG0480019509.01.T01 | MTR_7g012070 | 32.258 | 341 | 210 | 11 | 32 | 361 | 40 | 370 | 1.97e-45 | 160 |
| MsG0480019509.01.T01 | MTR_7g011900 | 31.953 | 338 | 215 | 9 | 32 | 362 | 40 | 369 | 6.15e-45 | 158 |
| MsG0480019509.01.T01 | MTR_1g096310 | 32.209 | 326 | 207 | 7 | 32 | 350 | 40 | 358 | 6.23e-45 | 158 |
| MsG0480019509.01.T01 | MTR_7g011990 | 30.904 | 343 | 209 | 10 | 32 | 362 | 40 | 366 | 1.20e-44 | 157 |
| MsG0480019509.01.T01 | MTR_1g036460 | 30.931 | 333 | 216 | 7 | 25 | 350 | 30 | 355 | 1.87e-44 | 157 |
| MsG0480019509.01.T01 | MTR_1g036490 | 30.259 | 347 | 226 | 8 | 25 | 362 | 30 | 369 | 1.28e-43 | 155 |
| MsG0480019509.01.T01 | MTR_8g068750 | 32.938 | 337 | 212 | 9 | 26 | 355 | 41 | 370 | 2.55e-43 | 154 |
| MsG0480019509.01.T01 | MTR_5g098170 | 34.132 | 334 | 199 | 12 | 31 | 355 | 33 | 354 | 3.35e-43 | 154 |
| MsG0480019509.01.T01 | MTR_7g012040 | 32.036 | 334 | 214 | 8 | 32 | 361 | 39 | 363 | 4.50e-43 | 153 |
| MsG0480019509.01.T01 | MTR_8g068690 | 33.231 | 325 | 204 | 8 | 26 | 344 | 40 | 357 | 1.76e-42 | 152 |
| MsG0480019509.01.T01 | MTR_0591s0010 | 32.923 | 325 | 205 | 8 | 26 | 344 | 41 | 358 | 6.82e-42 | 150 |
| MsG0480019509.01.T01 | MTR_7g012020 | 32.239 | 335 | 213 | 8 | 32 | 361 | 39 | 364 | 7.87e-42 | 150 |
| MsG0480019509.01.T01 | MTR_7g118300 | 31.751 | 337 | 206 | 9 | 26 | 353 | 42 | 363 | 5.42e-41 | 148 |
| MsG0480019509.01.T01 | MTR_2g055940 | 31.065 | 338 | 212 | 11 | 32 | 361 | 34 | 358 | 8.21e-41 | 147 |
| MsG0480019509.01.T01 | MTR_7g118320 | 31.611 | 329 | 212 | 8 | 26 | 348 | 26 | 347 | 1.32e-40 | 147 |
| MsG0480019509.01.T01 | MTR_2g072790 | 32.344 | 337 | 209 | 8 | 31 | 360 | 27 | 351 | 1.01e-39 | 144 |
| MsG0480019509.01.T01 | MTR_3g092900 | 32.203 | 354 | 206 | 11 | 9 | 349 | 17 | 349 | 3.30e-39 | 143 |
| MsG0480019509.01.T01 | MTR_1g036510 | 29.217 | 332 | 223 | 5 | 25 | 350 | 44 | 369 | 1.52e-38 | 142 |
| MsG0480019509.01.T01 | MTR_3g021440 | 31.804 | 327 | 211 | 8 | 28 | 350 | 38 | 356 | 1.09e-37 | 139 |
| MsG0480019509.01.T01 | MTR_8g024160 | 33.333 | 255 | 161 | 5 | 98 | 350 | 68 | 315 | 7.23e-35 | 130 |
| MsG0480019509.01.T01 | MTR_3g021430 | 30.031 | 323 | 214 | 8 | 32 | 350 | 29 | 343 | 2.08e-34 | 130 |
| MsG0480019509.01.T01 | MTR_3g096050 | 29.885 | 348 | 222 | 11 | 23 | 362 | 24 | 357 | 2.86e-34 | 129 |
| MsG0480019509.01.T01 | MTR_8g068570 | 31.511 | 311 | 173 | 9 | 26 | 330 | 41 | 317 | 4.74e-34 | 128 |
| MsG0480019509.01.T01 | MTR_4g038440 | 32.576 | 264 | 168 | 5 | 3 | 263 | 14 | 270 | 6.70e-34 | 126 |
| MsG0480019509.01.T01 | MTR_8g068730 | 29.586 | 338 | 190 | 10 | 26 | 355 | 41 | 338 | 3.17e-31 | 121 |
| MsG0480019509.01.T01 | MTR_6g078850 | 27.586 | 348 | 232 | 9 | 9 | 348 | 33 | 368 | 9.21e-31 | 122 |
| MsG0480019509.01.T01 | MTR_3g096070 | 27.907 | 344 | 207 | 10 | 23 | 360 | 24 | 332 | 1.17e-29 | 117 |
| MsG0480019509.01.T01 | MTR_7g012020 | 31.120 | 241 | 157 | 6 | 32 | 267 | 39 | 275 | 1.78e-27 | 110 |
| MsG0480019509.01.T01 | MTR_1g050220 | 33.117 | 154 | 90 | 2 | 194 | 347 | 147 | 287 | 5.12e-22 | 95.1 |
| MsG0480019509.01.T01 | MTR_8g068690 | 29.268 | 246 | 165 | 6 | 26 | 266 | 40 | 281 | 4.42e-21 | 92.4 |
| MsG0480019509.01.T01 | MTR_1g036510 | 28.862 | 246 | 164 | 6 | 25 | 263 | 44 | 285 | 4.33e-20 | 89.7 |
| MsG0480019509.01.T01 | MTR_3g096050 | 31.174 | 247 | 153 | 9 | 23 | 263 | 24 | 259 | 9.84e-20 | 88.2 |
| MsG0480019509.01.T01 | MTR_7g012020 | 31.429 | 210 | 135 | 6 | 32 | 236 | 39 | 244 | 2.32e-19 | 86.7 |
| MsG0480019509.01.T01 | MTR_8g068740 | 28.000 | 275 | 153 | 8 | 26 | 292 | 41 | 278 | 5.77e-19 | 86.3 |
| MsG0480019509.01.T01 | MTR_8g068600 | 28.448 | 232 | 157 | 6 | 38 | 264 | 27 | 254 | 2.74e-18 | 84.3 |
| MsG0480019509.01.T01 | MTR_8g068620 | 35.088 | 114 | 72 | 1 | 153 | 266 | 2 | 113 | 7.85e-15 | 70.5 |
| MsG0480019509.01.T01 | MTR_4g025570 | 41.748 | 103 | 50 | 4 | 6 | 108 | 16 | 108 | 2.66e-14 | 71.6 |
| MsG0480019509.01.T01 | MTR_4g025570 | 41.667 | 96 | 52 | 3 | 265 | 359 | 122 | 214 | 3.53e-13 | 68.6 |
| MsG0480019509.01.T01 | MTR_8g068760 | 40.000 | 95 | 52 | 3 | 263 | 355 | 135 | 226 | 9.23e-13 | 67.4 |
| MsG0480019509.01.T01 | MTR_1g036510 | 27.397 | 219 | 148 | 6 | 25 | 236 | 44 | 258 | 1.63e-12 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019509.01.T01 | AT4G35150 | 30.447 | 358 | 189 | 10 | 16 | 362 | 15 | 323 | 2.73e-48 | 166 |
| MsG0480019509.01.T01 | AT4G35160 | 33.151 | 365 | 213 | 12 | 16 | 362 | 29 | 380 | 1.67e-47 | 166 |
| MsG0480019509.01.T01 | AT5G54160 | 33.233 | 331 | 194 | 9 | 31 | 350 | 34 | 348 | 5.26e-43 | 153 |
| MsG0480019509.01.T01 | AT1G77520 | 30.728 | 371 | 232 | 11 | 8 | 362 | 19 | 380 | 1.32e-42 | 152 |
| MsG0480019509.01.T01 | AT1G51990 | 32.749 | 342 | 208 | 11 | 33 | 363 | 32 | 362 | 6.47e-42 | 150 |
| MsG0480019509.01.T01 | AT1G51990 | 32.749 | 342 | 208 | 11 | 33 | 363 | 32 | 362 | 3.40e-41 | 148 |
| MsG0480019509.01.T01 | AT3G53140 | 33.430 | 344 | 201 | 13 | 31 | 360 | 27 | 356 | 5.02e-39 | 142 |
| MsG0480019509.01.T01 | AT1G33030 | 29.518 | 332 | 215 | 10 | 31 | 353 | 21 | 342 | 3.05e-36 | 135 |
| MsG0480019509.01.T01 | AT5G53810 | 31.124 | 347 | 219 | 9 | 29 | 362 | 38 | 377 | 6.43e-36 | 134 |
| MsG0480019509.01.T01 | AT1G77530 | 31.536 | 371 | 229 | 11 | 8 | 362 | 19 | 380 | 1.67e-34 | 131 |
| MsG0480019509.01.T01 | AT1G63140 | 29.730 | 370 | 236 | 11 | 9 | 362 | 19 | 380 | 5.35e-34 | 129 |
| MsG0480019509.01.T01 | AT1G63140 | 29.730 | 370 | 236 | 11 | 9 | 362 | 39 | 400 | 6.83e-34 | 129 |
| MsG0480019509.01.T01 | AT1G76790 | 28.032 | 371 | 217 | 16 | 7 | 355 | 9 | 351 | 1.63e-26 | 108 |
| MsG0480019509.01.T01 | AT1G62900 | 35.938 | 192 | 118 | 3 | 173 | 362 | 16 | 204 | 8.59e-26 | 103 |
| MsG0480019509.01.T01 | AT1G21130 | 27.219 | 338 | 211 | 12 | 31 | 350 | 40 | 360 | 1.22e-24 | 103 |
| MsG0480019509.01.T01 | AT1G21100 | 27.083 | 336 | 214 | 11 | 31 | 350 | 40 | 360 | 4.93e-24 | 102 |
| MsG0480019509.01.T01 | AT1G21110 | 26.284 | 331 | 213 | 11 | 31 | 345 | 40 | 355 | 1.87e-23 | 100 |
| MsG0480019509.01.T01 | AT5G37170 | 24.033 | 362 | 221 | 10 | 8 | 362 | 19 | 333 | 1.01e-22 | 97.8 |
| MsG0480019509.01.T01 | AT5G37170 | 30.928 | 194 | 128 | 4 | 172 | 362 | 43 | 233 | 1.27e-22 | 95.5 |
| MsG0480019509.01.T01 | AT1G21120 | 25.893 | 336 | 218 | 11 | 31 | 350 | 40 | 360 | 2.55e-22 | 97.4 |
| MsG0480019509.01.T01 | AT1G21120 | 25.893 | 336 | 218 | 11 | 31 | 350 | 88 | 408 | 4.99e-22 | 97.1 |
| MsG0480019509.01.T01 | AT5G37170 | 33.526 | 173 | 106 | 5 | 194 | 360 | 14 | 183 | 8.47e-22 | 92.0 |
| MsG0480019509.01.T01 | AT1G63140 | 27.881 | 269 | 175 | 8 | 9 | 263 | 39 | 302 | 2.05e-16 | 79.3 |
| MsG0480019509.01.T01 | AT1G33030 | 26.778 | 239 | 162 | 7 | 31 | 263 | 21 | 252 | 3.41e-15 | 75.1 |
| MsG0480019509.01.T01 | AT1G33030 | 26.778 | 239 | 162 | 7 | 31 | 263 | 21 | 252 | 6.62e-15 | 74.7 |
Find 62 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGCAAGAGGAGAGACATTT+TGG | 0.134700 | 4:-21450678 | MsG0480019509.01.T01:CDS |
| ATGATGATGACTTTGTTAAA+TGG | 0.247172 | 4:-21449733 | MsG0480019509.01.T01:CDS |
| TGAAACAAATGAGTTGAAAT+TGG | 0.279186 | 4:-21449767 | MsG0480019509.01.T01:CDS |
| TGTATATATGTTTGTATAGA+TGG | 0.289789 | 4:+21451079 | None:intergenic |
| TGAATTGTCCTTAAGGATAT+TGG | 0.289913 | 4:-21449878 | MsG0480019509.01.T01:CDS |
| CTCAGTGAGCCATTTGTTAG+AGG | 0.332609 | 4:+21450726 | None:intergenic |
| CTTAACAAAGATTCTGAATC+TGG | 0.352374 | 4:-21450649 | MsG0480019509.01.T01:CDS |
| GCTAATTCAGTAAGAGTCAT+TGG | 0.368956 | 4:+21450982 | None:intergenic |
| TAGCCATGTGTTTGAAGGTT+TGG | 0.381600 | 4:-21450557 | MsG0480019509.01.T01:CDS |
| AAGAAAGAGAGAAGAAAGAA+TGG | 0.382447 | 4:-21449708 | MsG0480019509.01.T01:CDS |
| AACGAAAATTTGAAATTTGT+TGG | 0.401585 | 4:-21450415 | MsG0480019509.01.T01:CDS |
| TGAGGTGAGGAAAAGTTTCA+TGG | 0.410589 | 4:+21450485 | None:intergenic |
| CTGAGTTTCTTCCCTGCCAT+TGG | 0.421198 | 4:+21451119 | None:intergenic |
| GAGTGTAGCCATGTGTTTGA+AGG | 0.425506 | 4:-21450562 | MsG0480019509.01.T01:CDS |
| GTTTGAAGGTTTGGAGTCTC+TGG | 0.426025 | 4:-21450548 | MsG0480019509.01.T01:CDS |
| GGAATGATGAATTGTCCTTA+AGG | 0.441769 | 4:-21449885 | MsG0480019509.01.T01:CDS |
| CCACTGATGAGAAGCATTGA+AGG | 0.442792 | 4:+21450817 | None:intergenic |
| GCAGTTCTCCAATATCCTTA+AGG | 0.450113 | 4:+21449870 | None:intergenic |
| GTTGATGTTGGAGGTGGCAC+AGG | 0.461040 | 4:-21450526 | MsG0480019509.01.T01:CDS |
| CTCAAATCAGCCGTGGAACT+AGG | 0.484791 | 4:-21451036 | MsG0480019509.01.T01:CDS |
| AGAAAGAGAGAAGAAAGAAT+GGG | 0.494469 | 4:-21449707 | MsG0480019509.01.T01:CDS |
| TTGGAGTCTCTGGTTGATGT+TGG | 0.495110 | 4:-21450538 | MsG0480019509.01.T01:CDS |
| GAAAATTTGAAATTTGTTGG+TGG | 0.495217 | 4:-21450412 | MsG0480019509.01.T01:CDS |
| GGTCAAATACTATGCATTTG+AGG | 0.496242 | 4:+21450467 | None:intergenic |
| GAGTCTCTGGTTGATGTTGG+AGG | 0.503583 | 4:-21450535 | MsG0480019509.01.T01:CDS |
| GCAAACGCAAGAGACGATAA+AGG | 0.507273 | 4:+21450923 | None:intergenic |
| AGTCTCTTTGAGAGCGCAAG+AGG | 0.511386 | 4:-21450691 | MsG0480019509.01.T01:CDS |
| TGAAGCACTCCGCTAACAAA+TGG | 0.512286 | 4:+21450775 | None:intergenic |
| ACACTGATGAATGGCAAAGA+AGG | 0.516027 | 4:-21450871 | MsG0480019509.01.T01:CDS |
| CACGGCTGATTTGAGTGCCA+TGG | 0.517659 | 4:+21451044 | None:intergenic |
| TGTGGCACTCCTCTAACAAA+TGG | 0.518573 | 4:-21450735 | MsG0480019509.01.T01:CDS |
| GATGTTGGAGGTGGCACAGG+AGG | 0.520874 | 4:-21450523 | MsG0480019509.01.T01:CDS |
| ATCTGCTATGCCTAGTTCCA+CGG | 0.523313 | 4:+21451026 | None:intergenic |
| TGGATTTCAGTACCAATGGC+AGG | 0.523941 | 4:-21451131 | MsG0480019509.01.T01:CDS |
| TGTTTGTCACCATTTGTTAG+CGG | 0.537198 | 4:-21450784 | MsG0480019509.01.T01:CDS |
| ATAGACTTGAAACCGCATAT+AGG | 0.541344 | 4:+21449640 | None:intergenic |
| GCGAAAACAACACTGATGAA+TGG | 0.541866 | 4:-21450880 | MsG0480019509.01.T01:CDS |
| ATCCATGTCGTCTGAACGTG+TGG | 0.545741 | 4:-21450753 | MsG0480019509.01.T01:CDS |
| ATACAGCTTTATAAATTCCA+TGG | 0.553200 | 4:-21451061 | MsG0480019509.01.T01:CDS |
| TTGAGTAAAACAGCATCTGC+AGG | 0.557051 | 4:+21450367 | None:intergenic |
| AAAATGGATTTCAGTACCAA+TGG | 0.558143 | 4:-21451135 | MsG0480019509.01.T01:CDS |
| GAGTATGTTTCAAGATGCCA+TGG | 0.571374 | 4:-21450620 | MsG0480019509.01.T01:CDS |
| CCTTCAATGCTTCTCATCAG+TGG | 0.573536 | 4:-21450817 | MsG0480019509.01.T01:CDS |
| CAAATGGCTCACTGAGGACA+AGG | 0.577988 | 4:-21450719 | MsG0480019509.01.T01:CDS |
| GATTTGAGAATCAGCTGCCA+TGG | 0.583476 | 4:+21450603 | None:intergenic |
| TCTCTGGTTGATGTTGGAGG+TGG | 0.586831 | 4:-21450532 | MsG0480019509.01.T01:CDS |
| TGCCACACGTTCAGACGACA+TGG | 0.594397 | 4:+21450751 | None:intergenic |
| TAAAGGACACTAACTTTCGA+AGG | 0.602158 | 4:+21450940 | None:intergenic |
| TTGCGTTTGCTAACACACAA+TGG | 0.605432 | 4:-21450910 | MsG0480019509.01.T01:CDS |
| AATACTATGCATTTGAGGTG+AGG | 0.609052 | 4:+21450472 | None:intergenic |
| GGACACTAACTTTCGAAGGG+TGG | 0.614681 | 4:+21450944 | None:intergenic |
| AGTAAAACAGCATCTGCAGG+AGG | 0.624714 | 4:+21450370 | None:intergenic |
| GGATTTCAGTACCAATGGCA+GGG | 0.632276 | 4:-21451130 | MsG0480019509.01.T01:CDS |
| GAGAAACTCATATTTGACGC+AGG | 0.638948 | 4:-21449685 | MsG0480019509.01.T01:CDS |
| CATGGCACTCAAATCAGCCG+TGG | 0.640463 | 4:-21451043 | MsG0480019509.01.T01:CDS |
| GCAGATGCAATCCACAACCA+TGG | 0.642290 | 4:-21451009 | MsG0480019509.01.T01:CDS |
| TATAAGATTACACCTATATG+CGG | 0.657814 | 4:-21449652 | MsG0480019509.01.T01:CDS |
| CTGATGAGAAGCATTGAAGG+AGG | 0.666811 | 4:+21450820 | None:intergenic |
| AAAGGACACTAACTTTCGAA+GGG | 0.667819 | 4:+21450941 | None:intergenic |
| ACTCCAAACCTTCAAACACA+TGG | 0.677292 | 4:+21450554 | None:intergenic |
| GTAAAACAGCATCTGCAGGA+GGG | 0.681270 | 4:+21450371 | None:intergenic |
| CTCTAACAAATGGCTCACTG+AGG | 0.728882 | 4:-21450725 | MsG0480019509.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAAACATATTTCTTCTT+TGG | - | Chr4:21450432-21450451 | MsG0480019509.01.T01:CDS | 15.0% |
| !! | TTAAAACATATTTCTTCTTT+GGG | - | Chr4:21450433-21450452 | MsG0480019509.01.T01:CDS | 15.0% |
| !! | CTAATTAATCTTTGCAATAA+AGG | - | Chr4:21450738-21450757 | MsG0480019509.01.T01:CDS | 20.0% |
| !! | TGTATATATGTTTGTATAGA+TGG | + | Chr4:21449677-21449696 | None:intergenic | 20.0% |
| !!! | AACGAAAATTTGAAATTTGT+TGG | - | Chr4:21450338-21450357 | MsG0480019509.01.T01:intron | 20.0% |
| !!! | GAGTAATTTTCTTATGTTAT+TGG | - | Chr4:21450685-21450704 | MsG0480019509.01.T01:CDS | 20.0% |
| !!! | GATAAATTCTTTTTTTACAG+TGG | - | Chr4:21450832-21450851 | MsG0480019509.01.T01:CDS | 20.0% |
| ! | TAATTAGTCATCAACAAAAC+TGG | + | Chr4:21450725-21450744 | None:intergenic | 25.0% |
| ! | TATAAGATTACACCTATATG+CGG | - | Chr4:21451101-21451120 | MsG0480019509.01.T01:CDS | 25.0% |
| !! | AAAAAAGCTATTTCAGGAAA+AGG | - | Chr4:21450906-21450925 | MsG0480019509.01.T01:CDS | 25.0% |
| !! | AAAAAGCTATTTCAGGAAAA+GGG | - | Chr4:21450907-21450926 | MsG0480019509.01.T01:CDS | 25.0% |
| !! | ATACAGCTTTATAAATTCCA+TGG | - | Chr4:21449692-21449711 | MsG0480019509.01.T01:CDS | 25.0% |
| !! | ATGATGATGACTTTGTTAAA+TGG | - | Chr4:21451020-21451039 | MsG0480019509.01.T01:CDS | 25.0% |
| !! | TGAAACAAATGAGTTGAAAT+TGG | - | Chr4:21450986-21451005 | MsG0480019509.01.T01:CDS | 25.0% |
| !!! | GAAAATTTGAAATTTGTTGG+TGG | - | Chr4:21450341-21450360 | MsG0480019509.01.T01:intron | 25.0% |
| AAGAAAGAGAGAAGAAAGAA+TGG | - | Chr4:21451045-21451064 | MsG0480019509.01.T01:CDS | 30.0% | |
| AATTAATTGCCGCTGATATA+AGG | - | Chr4:21450637-21450656 | MsG0480019509.01.T01:CDS | 30.0% | |
| ACCAATCTTAAAATTGTCAG+TGG | - | Chr4:21450582-21450601 | MsG0480019509.01.T01:CDS | 30.0% | |
| AGAAAGAGAGAAGAAAGAAT+GGG | - | Chr4:21451046-21451065 | MsG0480019509.01.T01:CDS | 30.0% | |
| ATTTCAGGAAAAGGGAAAAA+AGG | - | Chr4:21450915-21450934 | MsG0480019509.01.T01:CDS | 30.0% | |
| CAAGTTGTAAAAAACATGTC+TGG | - | Chr4:21450314-21450333 | MsG0480019509.01.T01:intron | 30.0% | |
| CTTAACAAAGATTCTGAATC+TGG | - | Chr4:21450104-21450123 | MsG0480019509.01.T01:intron | 30.0% | |
| TATCGTTGAAAAATGTCAAG+CGG | + | Chr4:21450530-21450549 | None:intergenic | 30.0% | |
| TGAATTGTCCTTAAGGATAT+TGG | - | Chr4:21450875-21450894 | MsG0480019509.01.T01:CDS | 30.0% | |
| TGTTCATTACCTTATATCAG+CGG | + | Chr4:21450649-21450668 | None:intergenic | 30.0% | |
| ! | AACTGCAAAAAAGCTATTTC+AGG | - | Chr4:21450900-21450919 | MsG0480019509.01.T01:CDS | 30.0% |
| !!! | GACATGTTTTTTACAACTTG+TGG | + | Chr4:21450314-21450333 | None:intergenic | 30.0% |
| !!! | TGTTTTTTACAACTTGTGGT+TGG | + | Chr4:21450310-21450329 | None:intergenic | 30.0% |
| AAAGGACACTAACTTTCGAA+GGG | + | Chr4:21449815-21449834 | None:intergenic | 35.0% | |
| AGGAAAAGGGAAAAAAGGAA+AGG | - | Chr4:21450920-21450939 | MsG0480019509.01.T01:CDS | 35.0% | |
| ATAGACTTGAAACCGCATAT+AGG | + | Chr4:21451116-21451135 | None:intergenic | 35.0% | |
| GCTAATTCAGTAAGAGTCAT+TGG | + | Chr4:21449774-21449793 | None:intergenic | 35.0% | |
| GGAATGATGAATTGTCCTTA+AGG | - | Chr4:21450868-21450887 | MsG0480019509.01.T01:CDS | 35.0% | |
| GGTCAAATACTATGCATTTG+AGG | + | Chr4:21450289-21450308 | None:intergenic | 35.0% | |
| TAAAGGACACTAACTTTCGA+AGG | + | Chr4:21449816-21449835 | None:intergenic | 35.0% | |
| ! | AATACTATGCATTTGAGGTG+AGG | + | Chr4:21450284-21450303 | None:intergenic | 35.0% |
| ! | TGTTTGTCACCATTTGTTAG+CGG | - | Chr4:21449969-21449988 | MsG0480019509.01.T01:intron | 35.0% |
| !! | TACAGTGGATTTTGCATGAT+TGG | - | Chr4:21450847-21450866 | MsG0480019509.01.T01:CDS | 35.0% |
| !!! | GCCACTGACAATTTTAAGAT+TGG | + | Chr4:21450586-21450605 | None:intergenic | 35.0% |
| !!! | TAAGAGTCATTGGTTTTCCA+TGG | + | Chr4:21449764-21449783 | None:intergenic | 35.0% |
| ACACTGATGAATGGCAAAGA+AGG | - | Chr4:21449882-21449901 | MsG0480019509.01.T01:CDS | 40.0% | |
| ACTCCAAACCTTCAAACACA+TGG | + | Chr4:21450202-21450221 | None:intergenic | 40.0% | |
| GAGAAACTCATATTTGACGC+AGG | - | Chr4:21451068-21451087 | MsG0480019509.01.T01:CDS | 40.0% | |
| GAGTATGTTTCAAGATGCCA+TGG | - | Chr4:21450133-21450152 | MsG0480019509.01.T01:intron | 40.0% | |
| GCAGTTCTCCAATATCCTTA+AGG | + | Chr4:21450886-21450905 | None:intergenic | 40.0% | |
| GCGAAAACAACACTGATGAA+TGG | - | Chr4:21449873-21449892 | MsG0480019509.01.T01:CDS | 40.0% | |
| TAGCCATGTGTTTGAAGGTT+TGG | - | Chr4:21450196-21450215 | MsG0480019509.01.T01:intron | 40.0% | |
| TGAGGTGAGGAAAAGTTTCA+TGG | + | Chr4:21450271-21450290 | None:intergenic | 40.0% | |
| TGCTCATTCGCTTATATCAG+TGG | - | Chr4:21450494-21450513 | MsG0480019509.01.T01:CDS | 40.0% | |
| TTGAGTAAAACAGCATCTGC+AGG | + | Chr4:21450389-21450408 | None:intergenic | 40.0% | |
| ! | TTGCGTTTGCTAACACACAA+TGG | - | Chr4:21449843-21449862 | MsG0480019509.01.T01:CDS | 40.0% |
| !! | ACTGATCTTAAAAGCGTCAG+CGG | + | Chr4:21450559-21450578 | None:intergenic | 40.0% |
| !!! | TCATTGGTTTTCCATGGTTG+TGG | + | Chr4:21449758-21449777 | None:intergenic | 40.0% |
| !!! | TGCAGATGCTGTTTTACTCA+AGG | - | Chr4:21450388-21450407 | MsG0480019509.01.T01:CDS | 40.0% |
| AGCGGCAATTAATTGCCACT+CGG | + | Chr4:21450631-21450650 | None:intergenic | 45.0% | |
| AGTAAAACAGCATCTGCAGG+AGG | + | Chr4:21450386-21450405 | None:intergenic | 45.0% | |
| ATCTGCTATGCCTAGTTCCA+CGG | + | Chr4:21449730-21449749 | None:intergenic | 45.0% | |
| CCTTCAATGCTTCTCATCAG+TGG | - | Chr4:21449936-21449955 | MsG0480019509.01.T01:intron | 45.0% | |
| CTCTAACAAATGGCTCACTG+AGG | - | Chr4:21450028-21450047 | MsG0480019509.01.T01:intron | 45.0% | |
| GAGTGTAGCCATGTGTTTGA+AGG | - | Chr4:21450191-21450210 | MsG0480019509.01.T01:intron | 45.0% | |
| GATTTGAGAATCAGCTGCCA+TGG | + | Chr4:21450153-21450172 | None:intergenic | 45.0% | |
| GCAAACGCAAGAGACGATAA+AGG | + | Chr4:21449833-21449852 | None:intergenic | 45.0% | |
| GGATTTCAGTACCAATGGCA+GGG | - | Chr4:21449623-21449642 | MsG0480019509.01.T01:CDS | 45.0% | |
| GTAAAACAGCATCTGCAGGA+GGG | + | Chr4:21450385-21450404 | None:intergenic | 45.0% | |
| TGCCACTGAAAAATACCGAG+TGG | - | Chr4:21450613-21450632 | MsG0480019509.01.T01:CDS | 45.0% | |
| TGGATTTCAGTACCAATGGC+AGG | - | Chr4:21449622-21449641 | MsG0480019509.01.T01:CDS | 45.0% | |
| TGTGGCACTCCTCTAACAAA+TGG | - | Chr4:21450018-21450037 | MsG0480019509.01.T01:intron | 45.0% | |
| ! | CGCAAGAGGAGAGACATTTT+GGG | - | Chr4:21450076-21450095 | MsG0480019509.01.T01:intron | 45.0% |
| ! | CTCAGTGAGCCATTTGTTAG+AGG | + | Chr4:21450030-21450049 | None:intergenic | 45.0% |
| ! | GTTTGAAGGTTTGGAGTCTC+TGG | - | Chr4:21450205-21450224 | MsG0480019509.01.T01:intron | 45.0% |
| ! | TGAAGCACTCCGCTAACAAA+TGG | + | Chr4:21449981-21450000 | None:intergenic | 45.0% |
| ! | TTGGAGTCTCTGGTTGATGT+TGG | - | Chr4:21450215-21450234 | MsG0480019509.01.T01:intron | 45.0% |
| !! | CCACTGATGAGAAGCATTGA+AGG | + | Chr4:21449939-21449958 | None:intergenic | 45.0% |
| !! | CTGATGAGAAGCATTGAAGG+AGG | + | Chr4:21449936-21449955 | None:intergenic | 45.0% |
| !! | TGCCACTCGGTATTTTTCAG+TGG | + | Chr4:21450618-21450637 | None:intergenic | 45.0% |
| ATCCATGTCGTCTGAACGTG+TGG | - | Chr4:21450000-21450019 | MsG0480019509.01.T01:intron | 50.0% | |
| CAAATGGCTCACTGAGGACA+AGG | - | Chr4:21450034-21450053 | MsG0480019509.01.T01:intron | 50.0% | |
| CTCAAATCAGCCGTGGAACT+AGG | - | Chr4:21449717-21449736 | MsG0480019509.01.T01:CDS | 50.0% | |
| CTGAGTTTCTTCCCTGCCAT+TGG | + | Chr4:21449637-21449656 | None:intergenic | 50.0% | |
| GCAGATGCAATCCACAACCA+TGG | - | Chr4:21449744-21449763 | MsG0480019509.01.T01:CDS | 50.0% | |
| GGACACTAACTTTCGAAGGG+TGG | + | Chr4:21449812-21449831 | None:intergenic | 50.0% | |
| ! | AGTCTCTTTGAGAGCGCAAG+AGG | - | Chr4:21450062-21450081 | MsG0480019509.01.T01:intron | 50.0% |
| ! | GAGTCTCTGGTTGATGTTGG+AGG | - | Chr4:21450218-21450237 | MsG0480019509.01.T01:intron | 50.0% |
| ! | GCGCAAGAGGAGAGACATTT+TGG | - | Chr4:21450075-21450094 | MsG0480019509.01.T01:intron | 50.0% |
| ! | TCTCTGGTTGATGTTGGAGG+TGG | - | Chr4:21450221-21450240 | MsG0480019509.01.T01:intron | 50.0% |
| CATGGCACTCAAATCAGCCG+TGG | - | Chr4:21449710-21449729 | MsG0480019509.01.T01:CDS | 55.0% | |
| GTTGATGTTGGAGGTGGCAC+AGG | - | Chr4:21450227-21450246 | MsG0480019509.01.T01:intron | 55.0% | |
| TGCCACACGTTCAGACGACA+TGG | + | Chr4:21450005-21450024 | None:intergenic | 55.0% | |
| !! | CACGGCTGATTTGAGTGCCA+TGG | + | Chr4:21449712-21449731 | None:intergenic | 55.0% |
| GATGTTGGAGGTGGCACAGG+AGG | - | Chr4:21450230-21450249 | MsG0480019509.01.T01:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 21449621 | 21451154 | 21449621 | ID=MsG0480019509.01;Name=MsG0480019509.01 |
| Chr4 | mRNA | 21449621 | 21451154 | 21449621 | ID=MsG0480019509.01.T01;Parent=MsG0480019509.01;Name=MsG0480019509.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|363 |
| Chr4 | exon | 21450366 | 21451154 | 21450366 | ID=MsG0480019509.01.T01:exon:10343;Parent=MsG0480019509.01.T01 |
| Chr4 | exon | 21449621 | 21449923 | 21449621 | ID=MsG0480019509.01.T01:exon:10342;Parent=MsG0480019509.01.T01 |
| Chr4 | CDS | 21450366 | 21451154 | 21450366 | ID=MsG0480019509.01.T01:cds;Parent=MsG0480019509.01.T01 |
| Chr4 | CDS | 21449621 | 21449923 | 21449621 | ID=MsG0480019509.01.T01:cds;Parent=MsG0480019509.01.T01 |
| Gene Sequence |
| Protein sequence |
