AlfalfaGEDB | MsG0480019747.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019747.01

KEGG: K02925
GO: GO:0003735 GO:0005622 GO:0005840 GO:0006412
PFAM: PF00297

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019747.01.T03	WP_152306008.1	92.453	53	4	0	1	53	43	95	1.01E-24	97.4
MsG0480019747.01.T02	ADJ67187.1	51.351	74	36	0	1	74	17	90	1.65E-15	76.6
MsG0480019747.01.T04	ABK92574.1	87.5	40	5	0	1	40	24	63	4.70E-16	73.9
MsG0480019747.01.T01	XP_024642001.1	63.281	128	40	2	1	128	265	385	7.11E-44	159

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019747.01.T01	P22738	53.788	132	54	2	1	132	265	389	2.45E-42	147
MsG0480019747.01.T02	P22738	38.824	85	45	2	1	85	312	389	3.51E-11	61.6
MsG0480019747.01.T03	P35684	84.906	53	8	0	1	53	337	389	8.30E-24	94
MsG0480019747.01.T04	P35684	82.5	40	7	0	1	40	350	389	1.28E-15	70.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019747.01.T01	G7IBY1	63.281	128	40	2	1	128	265	385	3.40e-44	159
MsG0480019747.01.T02	E2CXH0	51.351	74	36	0	1	74	17	90	7.89e-16	76.6
MsG0480019747.01.T03	A9P871	88.679	53	6	0	1	53	11	63	5.18e-25	96.3
MsG0480019747.01.T03	G7IBY1	96.226	53	2	0	1	53	337	389	2.52e-24	102
MsG0480019747.01.T04	A9P871	87.500	40	5	0	1	40	24	63	2.24e-16	73.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180005110.01	MsG0480019747.01	0.827397	1.600432e-54	1.543412e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019747.01.T03	MTR_1g098540	96.226	53	2	0	1	53	337	389	6.38e-28	102
MsG0480019747.01.T03	MTR_4g094398	92.453	53	4	0	1	53	337	389	4.35e-27	100
MsG0480019747.01.T02	MTR_1g098540	43.210	81	39	2	1	81	312	385	5.66e-13	63.9
MsG0480019747.01.T04	MTR_1g098540	95.000	40	2	0	1	40	350	389	2.23e-19	78.6
MsG0480019747.01.T04	MTR_4g094398	92.500	40	3	0	1	40	350	389	7.39e-19	77.0
MsG0480019747.01.T01	MTR_1g098540	63.281	128	40	2	1	128	265	385	8.61e-48	159
MsG0480019747.01.T01	MTR_4g094398	55.469	128	50	2	1	128	265	385	8.33e-41	140

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019747.01.T03	AT1G61580	76.000	50	12	0	1	50	337	386	1.84e-20	82.0
MsG0480019747.01.T03	AT1G43170	74.510	51	13	0	1	51	254	304	7.58e-20	79.7
MsG0480019747.01.T03	AT1G43170	74.510	51	13	0	1	51	337	387	1.20e-19	79.7
MsG0480019747.01.T03	AT1G43170	74.510	51	13	0	1	51	337	387	1.20e-19	79.7
MsG0480019747.01.T03	AT1G43170	74.510	51	13	0	1	51	337	387	1.20e-19	79.7
MsG0480019747.01.T03	AT1G43170	74.510	51	13	0	1	51	337	387	1.20e-19	79.7
MsG0480019747.01.T03	AT1G43170	74.510	51	13	0	1	51	337	387	1.20e-19	79.7
MsG0480019747.01.T03	AT1G43170	74.510	51	13	0	1	51	337	387	1.20e-19	79.7
MsG0480019747.01.T03	AT1G43170	74.510	51	13	0	1	51	337	387	1.20e-19	79.7
MsG0480019747.01.T03	AT1G43170	74.510	51	13	0	1	51	337	387	1.20e-19	79.7
MsG0480019747.01.T02	AT1G61580	38.824	85	45	2	1	85	312	389	3.58e-12	61.6
MsG0480019747.01.T04	AT1G43170	71.053	38	11	0	1	38	267	304	1.46e-12	59.3
MsG0480019747.01.T04	AT1G43170	71.053	38	11	0	1	38	350	387	1.62e-12	59.3
MsG0480019747.01.T04	AT1G43170	71.053	38	11	0	1	38	350	387	1.62e-12	59.3
MsG0480019747.01.T04	AT1G43170	71.053	38	11	0	1	38	350	387	1.62e-12	59.3
MsG0480019747.01.T04	AT1G43170	71.053	38	11	0	1	38	350	387	1.62e-12	59.3
MsG0480019747.01.T04	AT1G43170	71.053	38	11	0	1	38	350	387	1.62e-12	59.3
MsG0480019747.01.T04	AT1G43170	71.053	38	11	0	1	38	350	387	1.62e-12	59.3
MsG0480019747.01.T04	AT1G43170	71.053	38	11	0	1	38	350	387	1.62e-12	59.3
MsG0480019747.01.T04	AT1G43170	71.053	38	11	0	1	38	350	387	1.62e-12	59.3
MsG0480019747.01.T04	AT1G61580	72.973	37	10	0	1	37	350	386	8.90e-11	54.3
MsG0480019747.01.T01	AT1G61580	53.788	132	54	2	1	132	265	389	2.50e-43	147
MsG0480019747.01.T01	AT1G43170	57.025	121	46	1	1	121	182	296	2.06e-42	143
MsG0480019747.01.T01	AT1G43170	57.025	121	46	1	1	121	265	379	3.58e-41	142
MsG0480019747.01.T01	AT1G43170	57.025	121	46	1	1	121	265	379	3.58e-41	142
MsG0480019747.01.T01	AT1G43170	57.025	121	46	1	1	121	265	379	3.58e-41	142
MsG0480019747.01.T01	AT1G43170	57.025	121	46	1	1	121	265	379	3.58e-41	142
MsG0480019747.01.T01	AT1G43170	57.025	121	46	1	1	121	265	379	3.58e-41	142
MsG0480019747.01.T01	AT1G43170	57.025	121	46	1	1	121	265	379	3.58e-41	142
MsG0480019747.01.T01	AT1G43170	57.025	121	46	1	1	121	265	379	3.58e-41	142
MsG0480019747.01.T01	AT1G43170	57.025	121	46	1	1	121	265	379	3.58e-41	142

Find 35 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGAGTAATAACCCTCTTCT+TGG	0.292723	4:+26309670	None:intergenic
ATCGATTCCTCTTCCAAGTT+TGG	0.294110	4:-26309587	MsG0480019747.01.T01:CDS
TCAGAGAAGCAAAAGTTCTA+TGG	0.364679	4:-26309542	MsG0480019747.01.T01:CDS
GACGCCTCAAAGCTTAATTA+TGG	0.367105	4:-26309520	MsG0480019747.01.T01:CDS
TTTGCTTCTCTGATGTTGTC+TGG	0.376238	4:+26309552	None:intergenic
GAAACGACCACGTCCAAACT+TGG	0.422838	4:+26309574	None:intergenic
TCTTTCACTGTTGCAAGAGC+TGG	0.431726	4:-26309883	MsG0480019747.01.T01:CDS
ATTTACAAGCTCGGCAAAGT+TGG	0.433680	4:-26309820	MsG0480019747.01.T01:CDS
TGAGAAAGACATTACACCTA+TGG	0.467448	4:-26309758	MsG0480019747.01.T01:CDS
ACTACCATAATTAAGCTTTG+AGG	0.473296	4:+26309516	None:intergenic
GACTTCTCGTCTTGCTCTTG+AGG	0.480840	4:-26309627	MsG0480019747.01.T01:CDS
TGTTCAGCTCAGTTCTGTGA+TGG	0.485951	4:+26309848	None:intergenic
AACAAGAAAATTTACAAGCT+CGG	0.491496	4:-26309829	MsG0480019747.01.T01:CDS
GACGAGAAGTCTGCTTAAGA+AGG	0.502167	4:+26309639	None:intergenic
TCCTCTTCCAAGTTTGGACG+TGG	0.513926	4:-26309581	MsG0480019747.01.T01:CDS
ATGATCAAGGGAGGCTGTGT+TGG	0.526401	4:-26309694	MsG0480019747.01.T01:CDS
CGAACTCAGTCTGAGCGCCA+TGG	0.544176	4:+26309788	None:intergenic
GGCTGTGTTGGTCCAAGAAG+AGG	0.556207	4:-26309682	MsG0480019747.01.T01:CDS
AGTACATAGTTTAAAACAGA+TGG	0.571470	4:+26309454	None:intergenic
GAACTCAGTCTGAGCGCCAT+GGG	0.574470	4:+26309789	None:intergenic
ACCACGTCCAAACTTGGAAG+AGG	0.581288	4:+26309580	None:intergenic
GCGCTCAGACTGAGTTCGAT+AGG	0.584674	4:-26309783	MsG0480019747.01.T01:CDS
GAGAAAGACATTACACCTAT+GGG	0.596080	4:-26309757	MsG0480019747.01.T01:CDS
ATGGGTGGTTTCCCACACTA+CGG	0.607609	4:-26309739	MsG0480019747.01.T01:CDS
ACGAGAAGTCTGCTTAAGAA+GGG	0.612938	4:+26309640	None:intergenic
CCTTGACAATACCGTAGTGT+GGG	0.616873	4:+26309728	None:intergenic
GTTGCAAGAGCTGGTCAGAA+CGG	0.617265	4:-26309874	MsG0480019747.01.T01:CDS
CAAGCTCGGCAAAGTTGGAG+AGG	0.619055	4:-26309815	MsG0480019747.01.T01:CDS
CCCACACTACGGTATTGTCA+AGG	0.620478	4:-26309728	MsG0480019747.01.T01:CDS
GCTGTGTTGGTCCAAGAAGA+GGG	0.623426	4:-26309681	MsG0480019747.01.T01:CDS
TAGTGTGGGAAACCACCCAT+AGG	0.626093	4:+26309742	None:intergenic
AAAGTTGGAGAGGAGACCCA+TGG	0.636220	4:-26309805	MsG0480019747.01.T01:CDS
TCCTTGACAATACCGTAGTG+TGG	0.648748	4:+26309727	None:intergenic
AAAGACATTACACCTATGGG+TGG	0.665025	4:-26309754	MsG0480019747.01.T01:CDS
ACACTACGGTATTGTCAAGG+AGG	0.722752	4:-26309725	MsG0480019747.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AACAAGAAAATTTACAAGCT+CGG	-	Chr4:26309477-26309496	MsG0480019747.01.T01:CDS	25.0%
!!	AGTACATAGTTTAAAACAGA+TGG	+	Chr4:26309855-26309874	None:intergenic	25.0%
	ACTACCATAATTAAGCTTTG+AGG	+	Chr4:26309793-26309812	None:intergenic	30.0%
!!!	GGTAGTAGTTTTGAAATGAT+GGG	-	Chr4:26309807-26309826	MsG0480019747.01.T01:CDS	30.0%
!!!	TGGTAGTAGTTTTGAAATGA+TGG	-	Chr4:26309806-26309825	MsG0480019747.01.T01:CDS	30.0%
	GAGAAAGACATTACACCTAT+GGG	-	Chr4:26309549-26309568	MsG0480019747.01.T01:CDS	35.0%
	TCAGAGAAGCAAAAGTTCTA+TGG	-	Chr4:26309764-26309783	MsG0480019747.01.T01:CDS	35.0%
	TGAGAAAGACATTACACCTA+TGG	-	Chr4:26309548-26309567	MsG0480019747.01.T01:CDS	35.0%
!	GAGGATTTTCTGATGATCAA+GGG	-	Chr4:26309600-26309619	MsG0480019747.01.T01:CDS	35.0%
	AAAGACATTACACCTATGGG+TGG	-	Chr4:26309552-26309571	MsG0480019747.01.T01:CDS	40.0%
	ACGAGAAGTCTGCTTAAGAA+GGG	+	Chr4:26309669-26309688	None:intergenic	40.0%
	ATTTACAAGCTCGGCAAAGT+TGG	-	Chr4:26309486-26309505	MsG0480019747.01.T01:CDS	40.0%
	CAGAGTAATAACCCTCTTCT+TGG	+	Chr4:26309639-26309658	None:intergenic	40.0%
	GACGCCTCAAAGCTTAATTA+TGG	-	Chr4:26309786-26309805	MsG0480019747.01.T01:CDS	40.0%
	TTTGCTTCTCTGATGTTGTC+TGG	+	Chr4:26309757-26309776	None:intergenic	40.0%
!	ATCGATTCCTCTTCCAAGTT+TGG	-	Chr4:26309719-26309738	MsG0480019747.01.T01:CDS	40.0%
!	GATTTTCTGATGATCAAGGG+AGG	-	Chr4:26309603-26309622	MsG0480019747.01.T01:CDS	40.0%
!	GGAGGATTTTCTGATGATCA+AGG	-	Chr4:26309599-26309618	MsG0480019747.01.T01:CDS	40.0%
	ACACTACGGTATTGTCAAGG+AGG	-	Chr4:26309581-26309600	MsG0480019747.01.T01:CDS	45.0%
	CCTTGACAATACCGTAGTGT+GGG	+	Chr4:26309581-26309600	None:intergenic	45.0%
	GACGAGAAGTCTGCTTAAGA+AGG	+	Chr4:26309670-26309689	None:intergenic	45.0%
	TCCTTGACAATACCGTAGTG+TGG	+	Chr4:26309582-26309601	None:intergenic	45.0%
	TGTTCAGCTCAGTTCTGTGA+TGG	+	Chr4:26309461-26309480	None:intergenic	45.0%
	AAAGTTGGAGAGGAGACCCA+TGG	-	Chr4:26309501-26309520	MsG0480019747.01.T01:CDS	50.0%
	ACCACGTCCAAACTTGGAAG+AGG	+	Chr4:26309729-26309748	None:intergenic	50.0%
	ATGATCAAGGGAGGCTGTGT+TGG	-	Chr4:26309612-26309631	MsG0480019747.01.T01:CDS	50.0%
	ATGGGTGGTTTCCCACACTA+CGG	-	Chr4:26309567-26309586	MsG0480019747.01.T01:CDS	50.0%
	CCCACACTACGGTATTGTCA+AGG	-	Chr4:26309578-26309597	MsG0480019747.01.T01:CDS	50.0%
	GAAACGACCACGTCCAAACT+TGG	+	Chr4:26309735-26309754	None:intergenic	50.0%
	GACTTCTCGTCTTGCTCTTG+AGG	-	Chr4:26309679-26309698	MsG0480019747.01.T01:CDS	50.0%
	GTTGCAAGAGCTGGTCAGAA+CGG	-	Chr4:26309432-26309451	MsG0480019747.01.T01:CDS	50.0%
	TAGTGTGGGAAACCACCCAT+AGG	+	Chr4:26309567-26309586	None:intergenic	50.0%
	TCCTCTTCCAAGTTTGGACG+TGG	-	Chr4:26309725-26309744	MsG0480019747.01.T01:CDS	50.0%
!	GCTGTGTTGGTCCAAGAAGA+GGG	-	Chr4:26309625-26309644	MsG0480019747.01.T01:CDS	50.0%
	CAAGCTCGGCAAAGTTGGAG+AGG	-	Chr4:26309491-26309510	MsG0480019747.01.T01:CDS	55.0%
	GAACTCAGTCTGAGCGCCAT+GGG	+	Chr4:26309520-26309539	None:intergenic	55.0%
	GCGCTCAGACTGAGTTCGAT+AGG	-	Chr4:26309523-26309542	MsG0480019747.01.T01:CDS	55.0%
!	GGCTGTGTTGGTCCAAGAAG+AGG	-	Chr4:26309624-26309643	MsG0480019747.01.T01:CDS	55.0%
	CGAACTCAGTCTGAGCGCCA+TGG	+	Chr4:26309521-26309540	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	26309426	26309902	26309426	ID=MsG0480019747.01;Name=MsG0480019747.01
Chr4	mRNA	26309426	26309902	26309426	ID=MsG0480019747.01.T01;Parent=MsG0480019747.01;Name=MsG0480019747.01.T01;_AED=0.33;_eAED=0.33;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|158
Chr4	exon	26309426	26309902	26309426	ID=MsG0480019747.01.T01:exon:9082;Parent=MsG0480019747.01.T01
Chr4	CDS	26309426	26309902	26309426	ID=MsG0480019747.01.T01:cds;Parent=MsG0480019747.01.T01
Chr4	mRNA	26309426	26309761	26309426	ID=MsG0480019747.01.T02;Parent=MsG0480019747.01;Name=MsG0480019747.01.T02;_AED=0.38;_eAED=0.38;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|111
Chr4	exon	26309426	26309761	26309426	ID=MsG0480019747.01.T02:exon:9083;Parent=MsG0480019747.01.T02
Chr4	CDS	26309426	26309761	26309426	ID=MsG0480019747.01.T02:cds;Parent=MsG0480019747.01.T02
Chr4	mRNA	26309526	26309687	26309526	ID=MsG0480019747.01.T03;Parent=MsG0480019747.01;Name=MsG0480019747.01.T03;_AED=0.44;_eAED=0.44;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|53
Chr4	exon	26309526	26309687	26309526	ID=MsG0480019747.01.T03:exon:9084;Parent=MsG0480019747.01.T03
Chr4	CDS	26309526	26309687	26309526	ID=MsG0480019747.01.T03:cds;Parent=MsG0480019747.01.T03
Chr4	mRNA	26309526	26309648	26309526	ID=MsG0480019747.01.T04;Parent=MsG0480019747.01;Name=MsG0480019747.01.T04;_AED=0.46;_eAED=0.46;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|40
Chr4	exon	26309526	26309648	26309526	ID=MsG0480019747.01.T04:exon:9085;Parent=MsG0480019747.01.T04
Chr4	CDS	26309526	26309648	26309526	ID=MsG0480019747.01.T04:cds;Parent=MsG0480019747.01.T04

Gene Sequence

>MsG0480019747.01.T03

AAGAGGGTTATTACTCTGCGTCAGTCCCTTCTTAAGCAGACTTCTCGTCTTGCTCTTGAGGATATCAAACTCAAGTTTATCGATTCCTCTTCCAAGTTTGGACGTGGTCGTTTCCAGACAACATCAGAGAAGCAAAAGTTCTATGGACGCCTCAAAGCTTAA

>MsG0480019747.01.T02

ATGGGTGGTTTCCCACACTACGGTATTGTCAAGGAGGATTTTCTGATGATCAAGGGAGGCTGTGTTGGTCCAAGAAGAGGGTTATTACTCTGCGTCAGTCCCTTCTTAAGCAGACTTCTCGTCTTGCTCTTGAGGATATCAAACTCAAGTTTATCGATTCCTCTTCCAAGTTTGGACGTGGTCGTTTCCAGACAACATCAGAGAAGCAAAAGTTCTATGGACGCCTCAAAGCTTAATTATGGTAGTAGTTTTGAAATGATGGGAACTTGCTTTTCATGTTTGTTACCATCTGTTTTAAACTATGTACTGTCTACTCTACATCTGACATTTTATTAA

>MsG0480019747.01.T04

ACTTCTCGTCTTGCTCTTGAGGATATCAAACTCAAGTTTATCGATTCCTCTTCCAAGTTTGGACGTGGTCGTTTCCAGACAACATCAGAGAAGCAAAAGTTCTATGGACGCCTCAAAGCTTAA

>MsG0480019747.01.T01

TTCACTGTTGCAAGAGCTGGTCAGAACGGATACCATCACAGAACTGAGCTGAACAAGAAAATTTACAAGCTCGGCAAAGTTGGAGAGGAGACCCATGGCGCTCAGACTGAGTTCGATAGGACTGAGAAAGACATTACACCTATGGGTGGTTTCCCACACTACGGTATTGTCAAGGAGGATTTTCTGATGATCAAGGGAGGCTGTGTTGGTCCAAGAAGAGGGTTATTACTCTGCGTCAGTCCCTTCTTAAGCAGACTTCTCGTCTTGCTCTTGAGGATATCAAACTCAAGTTTATCGATTCCTCTTCCAAGTTTGGACGTGGTCGTTTCCAGACAACATCAGAGAAGCAAAAGTTCTATGGACGCCTCAAAGCTTAATTATGGTAGTAGTTTTGAAATGATGGGAACTTGCTTTTCATGTTTGTTACCATCTGTTTTAAACTATGTACTGTCTACTCTACATCTGACATTTTATTAA

Protein sequence

>MsG0480019747.01.T03

KRVITLRQSLLKQTSRLALEDIKLKFIDSSSKFGRGRFQTTSEKQKFYGRLKA*

>MsG0480019747.01.T02

MGGFPHYGIVKEDFLMIKGGCVGPRRGLLLCVSPFLSRLLVLLLRISNSSLSIPLPSLDVVVSRQHQRSKSSMDASKLNYGSSFEMMGTCFSCLLPSVLNYVLSTLHLTFY*

>MsG0480019747.01.T04

TSRLALEDIKLKFIDSSSKFGRGRFQTTSEKQKFYGRLKA*

>MsG0480019747.01.T01

FTVARAGQNGYHHRTELNKKIYKLGKVGEETHGAQTEFDRTEKDITPMGGFPHYGIVKEDFLMIKGGCVGPRRGLLLCVSPFLSRLLVLLLRISNSSLSIPLPSLDVVVSRQHQRSKSSMDASKLNYGSSFEMMGTCFSCLLPSVLNYVLSTLHLTFY*