AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005110.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005110.01.T01 MTR_1g098540 64.804 358 33 4 27 313 37 372 6.13e-139 399
MsG0180005110.01.T01 MTR_4g094398 58.939 358 54 4 27 313 37 372 3.39e-125 364
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005110.01.T01 AT1G43170 59.587 339 47 3 43 313 56 372 6.92e-122 356
MsG0180005110.01.T01 AT1G43170 59.587 339 47 3 43 313 56 372 6.92e-122 356
MsG0180005110.01.T01 AT1G43170 59.587 339 47 3 43 313 56 372 6.92e-122 356
MsG0180005110.01.T01 AT1G43170 59.587 339 47 3 43 313 56 372 6.92e-122 356
MsG0180005110.01.T01 AT1G43170 59.587 339 47 3 43 313 56 372 6.92e-122 356
MsG0180005110.01.T01 AT1G43170 59.587 339 47 3 43 313 56 372 6.92e-122 356
MsG0180005110.01.T01 AT1G43170 59.587 339 47 3 43 313 56 372 6.92e-122 356
MsG0180005110.01.T01 AT1G43170 59.587 339 47 3 43 313 56 372 6.92e-122 356
MsG0180005110.01.T01 AT1G61580 56.425 358 63 4 27 313 37 372 5.09e-119 349
MsG0180005110.01.T01 AT1G43170 58.472 301 35 3 81 313 11 289 4.23e-112 328

Find 95 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATCAAACACAAGGAATT+TGG 0.166951 1:+87111173 MsG0180005110.01.T01:CDS
TAACTTGAATCTCCATGATA+TGG 0.252209 1:-87113770 None:intergenic
ACCTCTTCCAAGCTTAATTA+TGG 0.263224 1:+87114489 MsG0180005110.01.T01:CDS
AACAAAAGGTTGGATAAGTT+TGG 0.304502 1:-87114280 None:intergenic
AGTTTGAAGTACCGGTAAAT+TGG 0.321449 1:-87111232 None:intergenic
CAGAGTAATAACCATCTTCT+TGG 0.338520 1:-87114400 None:intergenic
TTGAGGAAGGTTGCATGTAT+TGG 0.358668 1:+87113977 MsG0180005110.01.T01:CDS
TAATCAAACACAAGGAATTT+GGG 0.376820 1:+87111174 MsG0180005110.01.T01:CDS
CGAAGGTGTTGTTACCCGTT+GGG 0.392699 1:+87113919 MsG0180005110.01.T01:CDS
CTCACCTATCGAATTCAGTC+TGG 0.395456 1:-87114126 None:intergenic
TCTTTCACTGTTGCCAGAGC+TGG 0.398591 1:+87114022 MsG0180005110.01.T01:CDS
GAATCTAATATCCAATTTAC+CGG 0.404584 1:+87111221 MsG0180005110.01.T01:CDS
ATATTAGATTCAGGAGTTGT+AGG 0.412423 1:-87111209 None:intergenic
GTAAATTGGATATTAGATTC+AGG 0.416938 1:-87111218 None:intergenic
CGAATTCAGTCTGGGCGTCA+TGG 0.430732 1:-87114117 None:intergenic
ATTTACAAGCTCGGCAAAGT+TGG 0.433680 1:+87114085 MsG0180005110.01.T01:CDS
AACTTGAATCTCCATGATAT+GGG 0.434515 1:-87113769 None:intergenic
TTCTCAGTCCTGTTAACAAA+AGG 0.439973 1:-87114294 None:intergenic
ATGGTTGTTGTCGGTGTTGT+GGG 0.442307 1:+87112833 MsG0180005110.01.T01:CDS
TTGTGTTTGATTATATCAAA+TGG 0.443710 1:-87111164 None:intergenic
GTAAGACTCACAGGGGTTTG+AGG 0.444999 1:+87113960 MsG0180005110.01.T01:CDS
GACTTCTTGTCTTGCTCTTG+AGG 0.449539 1:+87114443 MsG0180005110.01.T01:CDS
ATCTCAGTTTAGGAAAATGA+AGG 0.456653 1:+87113728 MsG0180005110.01.T01:intron
GATGAAATGATTGACATCAT+TGG 0.465546 1:+87113875 MsG0180005110.01.T01:CDS
TGAGAAAGACATTACACCTA+TGG 0.467448 1:+87114311 MsG0180005110.01.T01:CDS
GTCTTCCTTGTAAGACTCAC+AGG 0.467790 1:+87113951 MsG0180005110.01.T01:CDS
CAGTCCTGTTAACAAAAGGT+TGG 0.479299 1:-87114290 None:intergenic
CATGGTTGTTGTCGGTGTTG+TGG 0.479589 1:+87112832 MsG0180005110.01.T01:CDS
AAGACGAGTAACACCCCAAC+GGG 0.480535 1:-87113933 None:intergenic
ACGAAGGTGTTGTTACCCGT+TGG 0.482649 1:+87113918 MsG0180005110.01.T01:CDS
TGTTCAGCTCAGTTCTGTGA+TGG 0.485951 1:-87114057 None:intergenic
AGGGGTCTCAATGATTGTCT+CGG 0.487731 1:-87112807 None:intergenic
TATTTCAGAGCTTCACAAGA+AGG 0.489034 1:+87112772 MsG0180005110.01.T01:intron
GACAAGAAGTCTGCTTAAGA+AGG 0.489085 1:-87114431 None:intergenic
AACAAGAAAATTTACAAGCT+CGG 0.491496 1:+87114076 MsG0180005110.01.T01:CDS
ATCATGGAGATTCAAGTTAA+CGG 0.493946 1:+87113774 MsG0180005110.01.T01:CDS
AGAGTAATAACCATCTTCTT+GGG 0.500017 1:-87114399 None:intergenic
GGGGTCTCAATGATTGTCTC+GGG 0.500684 1:-87112806 None:intergenic
ACTACTACCATAATTAAGCT+TGG 0.501617 1:-87114496 None:intergenic
CATTGGAGGGACCAATGGTA+AGG 0.503304 1:+87113892 MsG0180005110.01.T01:CDS
ACCCCTCCCATGGTTGTTGT+CGG 0.508755 1:+87112824 MsG0180005110.01.T01:CDS
GTATCCGTTCTGACCAGCTC+TGG 0.518044 1:-87114035 None:intergenic
ATGATCAAGGGAGGCTGTGT+TGG 0.526401 1:+87114375 MsG0180005110.01.T01:CDS
GCAGAAGAAAGCCCATATCA+TGG 0.526839 1:+87113758 MsG0180005110.01.T01:CDS
GTTCAATGTTCTGAGACCAC+TGG 0.535547 1:-87112870 None:intergenic
CTGTGTTGGTCCCAAGAAGA+TGG 0.542037 1:+87114389 MsG0180005110.01.T01:CDS
CACAACACCGACAACAACCA+TGG 0.549447 1:-87112831 None:intergenic
AAACCCCTGTGAGTCTTACA+AGG 0.550558 1:-87113956 None:intergenic
ACCATAATTAAGCTTGGAAG+AGG 0.552858 1:-87114490 None:intergenic
GAAATGATTGACATCATTGG+AGG 0.556115 1:+87113878 MsG0180005110.01.T01:CDS
ACCGACAACAACCATGGGAG+GGG 0.558376 1:-87112825 None:intergenic
ACCTTCGTAACCCTTACCAT+TGG 0.560755 1:-87113903 None:intergenic
GACATCATTGGAGGGACCAA+TGG 0.560845 1:+87113887 MsG0180005110.01.T01:CDS
GAATTCAGTCTGGGCGTCAT+GGG 0.561506 1:-87114116 None:intergenic
TTCAATGTTCTGAGACCACT+GGG 0.562697 1:-87112869 None:intergenic
TCTTCCTTGTAAGACTCACA+GGG 0.563383 1:+87113952 MsG0180005110.01.T01:CDS
AGGTTGCATGTATTGGAGCT+TGG 0.565352 1:+87113984 MsG0180005110.01.T01:CDS
GACTCACAGGGGTTTGAGGA+AGG 0.568790 1:+87113964 MsG0180005110.01.T01:CDS
AGTACATAGTTTAAAACAGA+TGG 0.571470 1:-87114556 None:intergenic
GTACAAGGAGTTTGAAGTAC+CGG 0.574791 1:-87111240 None:intergenic
CGGTGGTACAATCGCACAGA+AGG 0.576143 1:+87113794 MsG0180005110.01.T01:CDS
AAGTCTGCTTAAGAAGGGAC+TGG 0.577488 1:-87114425 None:intergenic
TCTCAGTTTAGGAAAATGAA+GGG 0.582656 1:+87113729 MsG0180005110.01.T01:intron
ACAAGAAGTCTGCTTAAGAA+GGG 0.586428 1:-87114430 None:intergenic
GAAGGTGTTGTTACCCGTTG+GGG 0.586487 1:+87113920 MsG0180005110.01.T01:CDS
CACCGACAACAACCATGGGA+GGG 0.590277 1:-87112826 None:intergenic
GAGAAAGACATTACACCTAT+GGG 0.596080 1:+87114312 MsG0180005110.01.T01:CDS
ACAACACCGACAACAACCAT+GGG 0.596534 1:-87112830 None:intergenic
CGAGTTAATAAAATGTCAGA+CGG 0.600499 1:-87114583 None:intergenic
TCACCTATCGAATTCAGTCT+GGG 0.605778 1:-87114125 None:intergenic
ATGGGTGGTTTCCCACACTA+CGG 0.607609 1:+87114330 MsG0180005110.01.T01:CDS
ACAGTGAAAGAAACTCTAGC+AGG 0.609701 1:-87114010 None:intergenic
CCTTGACAATACCGTAGTGT+GGG 0.616873 1:-87114341 None:intergenic
ACGCCCAGACTGAATTCGAT+AGG 0.617003 1:+87114122 MsG0180005110.01.T01:CDS
GTTGCCAGAGCTGGTCAGAA+CGG 0.617265 1:+87114031 MsG0180005110.01.T01:CDS
CAAGCTCGGCAAAGTTGGAG+AGG 0.619055 1:+87114090 MsG0180005110.01.T01:CDS
CCCACACTACGGTATTGTCA+AGG 0.620478 1:+87114341 MsG0180005110.01.T01:CDS
AATCATTGAGACCCCTCCCA+TGG 0.622470 1:+87112814 MsG0180005110.01.T01:CDS
AAATGATTGACATCATTGGA+GGG 0.625317 1:+87113879 MsG0180005110.01.T01:CDS
TAGTGTGGGAAACCACCCAT+AGG 0.626093 1:-87114327 None:intergenic
ACACCGACAACAACCATGGG+AGG 0.629529 1:-87112827 None:intergenic
ATTGGAGGGACCAATGGTAA+GGG 0.630038 1:+87113893 MsG0180005110.01.T01:CDS
AGATTCAGGAGTTGTAGGCA+AGG 0.633513 1:-87111204 None:intergenic
TCCTTGACAATACCGTAGTG+TGG 0.648748 1:-87114342 None:intergenic
TCAGAACATTGAACACTGTG+TGG 0.651391 1:+87112879 MsG0180005110.01.T01:CDS
ATTTGATATAATCAAACACA+AGG 0.654528 1:+87111166 MsG0180005110.01.T01:CDS
ACCAATGGTAAGGGTTACGA+AGG 0.655109 1:+87113902 MsG0180005110.01.T01:CDS
AAAGACATTACACCTATGGG+TGG 0.665025 1:+87114315 MsG0180005110.01.T01:CDS
GAAGACGAGTAACACCCCAA+CGG 0.699527 1:-87113934 None:intergenic
CTTCCTTGTAAGACTCACAG+GGG 0.719058 1:+87113953 MsG0180005110.01.T01:CDS
ACACTACGGTATTGTCAAGG+AGG 0.722752 1:+87114344 MsG0180005110.01.T01:CDS
GTGGACTCAGCATTTGAGTG+AGG 0.740363 1:+87112898 MsG0180005110.01.T01:CDS
ATGGAGATTCAAGTTAACGG+TGG 0.740732 1:+87113777 MsG0180005110.01.T01:CDS
GGTTATGTGAAAACTCCCAG+TGG 0.779360 1:+87112854 MsG0180005110.01.T01:CDS
TATCAGCAGTAACTACTACG+TGG 0.812610 1:+87111141 MsG0180005110.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTTTTAAAAAAATT+TGG + Chr1:87113253-87113272 MsG0180005110.01.T01:intron 0.0%
!!! TTGTACTAATTTTTTTTTTT+CGG - Chr1:87111814-87111833 None:intergenic 10.0%
!!! AAATATATTTCTGAAAATCA+GGG - Chr1:87112741-87112760 None:intergenic 15.0%
!!! TAAATATATTTCTGAAAATC+AGG - Chr1:87112742-87112761 None:intergenic 15.0%
!!! TAATTTGGAATATATTTTGA+AGG + Chr1:87113084-87113103 MsG0180005110.01.T01:intron 15.0%
!! AAAACAGAAATCATAACAAA+AGG - Chr1:87113406-87113425 None:intergenic 20.0%
!! AAACAGAAATCATAACAAAA+GGG - Chr1:87113405-87113424 None:intergenic 20.0%
!! AAATTACAACTACTTTGAAT+GGG - Chr1:87113070-87113089 None:intergenic 20.0%
!! ACAAATATAAACAACATTCA+AGG - Chr1:87111947-87111966 None:intergenic 20.0%
!! ATATTACAATTCAGTGTTTA+TGG + Chr1:87114176-87114195 MsG0180005110.01.T01:intron 20.0%
!! ATTTGATATAATCAAACACA+AGG + Chr1:87111166-87111185 MsG0180005110.01.T01:CDS 20.0%
!! CAAATATAAACAACATTCAA+GGG - Chr1:87111946-87111965 None:intergenic 20.0%
!! TAAAAAATAAACAGCACATT+GGG - Chr1:87113034-87113053 None:intergenic 20.0%
!! TCAAAATAAAGTTCAAGAAA+CGG - Chr1:87113330-87113349 None:intergenic 20.0%
!! TTGTGTTTGATTATATCAAA+TGG - Chr1:87111167-87111186 None:intergenic 20.0%
!!! AAATCAAAAACTTGTTATCA+AGG - Chr1:87112096-87112115 None:intergenic 20.0%
!!! AATATATTTCTGAAAATCAG+GGG - Chr1:87112740-87112759 None:intergenic 20.0%
!!! AATGTCATAATTTTCACAAT+AGG + Chr1:87113602-87113621 MsG0180005110.01.T01:intron 20.0%
!!! TTTCTTGAACTTTATTTTGA+AGG + Chr1:87113330-87113349 MsG0180005110.01.T01:intron 20.0%
! AAAACATTAAAGCAGATATC+AGG + Chr1:87111469-87111488 MsG0180005110.01.T01:intron 25.0%
! AACAAGAAAATTTACAAGCT+CGG + Chr1:87114076-87114095 MsG0180005110.01.T01:CDS 25.0%
! ATAATCAAACACAAGGAATT+TGG + Chr1:87111173-87111192 MsG0180005110.01.T01:CDS 25.0%
! ATATTAGGATCTGATGAAAT+TGG - Chr1:87111706-87111725 None:intergenic 25.0%
! CAAATTACAACTACTTTGAA+TGG - Chr1:87113071-87113090 None:intergenic 25.0%
! CATTCAAAGTAGTTGTAATT+TGG + Chr1:87113069-87113088 MsG0180005110.01.T01:intron 25.0%
! GAATCTAATATCCAATTTAC+CGG + Chr1:87111221-87111240 MsG0180005110.01.T01:CDS 25.0%
! GTAAAAAATAAACAGCACAT+TGG - Chr1:87113035-87113054 None:intergenic 25.0%
! GTAAATTGGATATTAGATTC+AGG - Chr1:87111221-87111240 None:intergenic 25.0%
! GTATGTAGATTAAGTATTCT+TGG - Chr1:87111407-87111426 None:intergenic 25.0%
! GTGTTATCTCAATTGTAATT+TGG + Chr1:87112134-87112153 MsG0180005110.01.T01:intron 25.0%
! TAATCAAACACAAGGAATTT+GGG + Chr1:87111174-87111193 MsG0180005110.01.T01:CDS 25.0%
! TGTTTGATTATATCTTGACA+TGG + Chr1:87112454-87112473 MsG0180005110.01.T01:intron 25.0%
! TTCATGCTATATCTAGAAAA+TGG + Chr1:87111366-87111385 MsG0180005110.01.T01:intron 25.0%
!! AGTACATAGTTTAAAACAGA+TGG - Chr1:87114559-87114578 None:intergenic 25.0%
!! CATATTTTTCTATAACAGAG+AGG + Chr1:87111904-87111923 MsG0180005110.01.T01:intron 25.0%
!!! TTTGGAATATATTTTGAAGG+TGG + Chr1:87113087-87113106 MsG0180005110.01.T01:intron 25.0%
AAAAATGTAGTCACCGTTAA+TGG + Chr1:87113291-87113310 MsG0180005110.01.T01:intron 30.0%
AAAGTGTATATGCACTCTTA+CGG - Chr1:87111289-87111308 None:intergenic 30.0%
AAATGATTGACATCATTGGA+GGG + Chr1:87113879-87113898 MsG0180005110.01.T01:CDS 30.0%
AACAAAAGGTTGGATAAGTT+TGG - Chr1:87114283-87114302 None:intergenic 30.0%
AACTTGAATCTCCATGATAT+GGG - Chr1:87113772-87113791 None:intergenic 30.0%
AATCTAATGCCTTCTTATTG+AGG + Chr1:87112696-87112715 MsG0180005110.01.T01:intron 30.0%
ACTACTACCATAATTAAGCT+TGG - Chr1:87114499-87114518 None:intergenic 30.0%
AGAGTAATAACCATCTTCTT+GGG - Chr1:87114402-87114421 None:intergenic 30.0%
AGATTCCATGTAAAAGTAAG+AGG - Chr1:87112681-87112700 None:intergenic 30.0%
AGCTCAAATGACATATTCAT+AGG - Chr1:87112036-87112055 None:intergenic 30.0%
AGTGACAAAACTATACAGTT+AGG - Chr1:87112535-87112554 None:intergenic 30.0%
ATATTAGATTCAGGAGTTGT+AGG - Chr1:87111212-87111231 None:intergenic 30.0%
ATCTCAGTTTAGGAAAATGA+AGG + Chr1:87113728-87113747 MsG0180005110.01.T01:intron 30.0%
CTCAAATGACATATTCATAG+GGG - Chr1:87112034-87112053 None:intergenic 30.0%
GATGAAATGATTGACATCAT+TGG + Chr1:87113875-87113894 MsG0180005110.01.T01:CDS 30.0%
GCTATATCTAGAAAATGGTA+AGG + Chr1:87111371-87111390 MsG0180005110.01.T01:intron 30.0%
GCTCAAATGACATATTCATA+GGG - Chr1:87112035-87112054 None:intergenic 30.0%
GTTAACCGTTGAGATATAAA+AGG - Chr1:87113375-87113394 None:intergenic 30.0%
GTTTATCCTTATCTCAGTTT+AGG + Chr1:87113718-87113737 MsG0180005110.01.T01:intron 30.0%
TAACTTGAATCTCCATGATA+TGG - Chr1:87113773-87113792 None:intergenic 30.0%
TCTCAGTTTAGGAAAATGAA+GGG + Chr1:87113729-87113748 MsG0180005110.01.T01:intron 30.0%
TTGACAAGAATGATACTTAC+TGG - Chr1:87112292-87112311 None:intergenic 30.0%
TTGTAACTACCTCAATAAGA+AGG - Chr1:87112708-87112727 None:intergenic 30.0%
! ATCATGGAGATTCAAGTTAA+CGG + Chr1:87113774-87113793 MsG0180005110.01.T01:CDS 30.0%
! CATTTTCCTAAACTGAGATA+AGG - Chr1:87113727-87113746 None:intergenic 30.0%
! TACTGCCTTTTATATCTCAA+CGG + Chr1:87113367-87113386 MsG0180005110.01.T01:intron 30.0%
! TGCTTTAATGTTTTCGATTC+AGG - Chr1:87111463-87111482 None:intergenic 30.0%
! TTATCCAACCTTTTGTTAAC+AGG + Chr1:87114286-87114305 MsG0180005110.01.T01:CDS 30.0%
! TTCAGCTGAAAACGAATTTT+AGG - Chr1:87112621-87112640 None:intergenic 30.0%
!! CAAGAGATTTTGTCATTTCA+CGG - Chr1:87111265-87111284 None:intergenic 30.0%
!!! ATTTTCGTTTTTTAACGTCG+TGG + Chr1:87112354-87112373 MsG0180005110.01.T01:intron 30.0%
!!! GGAAGCATCTTTTGAATATT+AGG - Chr1:87111721-87111740 None:intergenic 30.0%
!!! GGTAGTAGTTTTGAAATGTT+GGG + Chr1:87114510-87114529 MsG0180005110.01.T01:CDS 30.0%
!!! TGGTAGTAGTTTTGAAATGT+TGG + Chr1:87114509-87114528 MsG0180005110.01.T01:CDS 30.0%
AAGAGTTGTTGACCATACAT+AGG - Chr1:87113517-87113536 None:intergenic 35.0%
ACAAGAAGTCTGCTTAAGAA+GGG - Chr1:87114433-87114452 None:intergenic 35.0%
ACCATAATTAAGCTTGGAAG+AGG - Chr1:87114493-87114512 None:intergenic 35.0%
ACCTCTTCCAAGCTTAATTA+TGG + Chr1:87114489-87114508 MsG0180005110.01.T01:CDS 35.0%
AGATTTCTGACTCCTATGTA+TGG + Chr1:87113502-87113521 MsG0180005110.01.T01:intron 35.0%
ATGCAAACTCAGCACTAATT+AGG + Chr1:87113455-87113474 MsG0180005110.01.T01:intron 35.0%
CAAATGACTGAATGAAACAC+AGG - Chr1:87111973-87111992 None:intergenic 35.0%
CAGAGTAATAACCATCTTCT+TGG - Chr1:87114403-87114422 None:intergenic 35.0%
CGTACCATAAATAGTTGCTT+AGG - Chr1:87111571-87111590 None:intergenic 35.0%
CTTGCAGTAGATTTCAGTTT+AGG + Chr1:87114225-87114244 MsG0180005110.01.T01:intron 35.0%
GAAATGATTGACATCATTGG+AGG + Chr1:87113878-87113897 MsG0180005110.01.T01:CDS 35.0%
GAATTTGACTAATGCAGTGA+AGG + Chr1:87112166-87112185 MsG0180005110.01.T01:intron 35.0%
GAGAAAGACATTACACCTAT+GGG + Chr1:87114312-87114331 MsG0180005110.01.T01:CDS 35.0%
GCTTCCTAAGCAACTATTTA+TGG + Chr1:87111564-87111583 MsG0180005110.01.T01:intron 35.0%
TAAGCGTATGAAAGTGATGA+GGG + Chr1:87112925-87112944 MsG0180005110.01.T01:intron 35.0%
TATTTCAGAGCTTCACAAGA+AGG + Chr1:87112772-87112791 MsG0180005110.01.T01:intron 35.0%
TGAGAAAGACATTACACCTA+TGG + Chr1:87114311-87114330 MsG0180005110.01.T01:CDS 35.0%
TGTCAATCATTTCATCCTTC+TGG - Chr1:87113871-87113890 None:intergenic 35.0%
TTAAGCGTATGAAAGTGATG+AGG + Chr1:87112924-87112943 MsG0180005110.01.T01:intron 35.0%
TTCTCAGTCCTGTTAACAAA+AGG - Chr1:87114297-87114316 None:intergenic 35.0%
TTGAGAGAAATCATGAAAGC+TGG - Chr1:87111853-87111872 None:intergenic 35.0%
TTTACAAGCAAGAGACTTTC+TGG + Chr1:87111508-87111527 MsG0180005110.01.T01:intron 35.0%
TTTCATTCAGTCATTTGAGC+TGG + Chr1:87111976-87111995 MsG0180005110.01.T01:intron 35.0%
! AATTCGTTTTCAGCTGAATC+TGG + Chr1:87112623-87112642 MsG0180005110.01.T01:intron 35.0%
! ATACAGTTTCTTTGAGAAGC+AGG + Chr1:87113826-87113845 MsG0180005110.01.T01:intron 35.0%
! GACATATTCATAGGGGTTTT+AGG - Chr1:87112027-87112046 None:intergenic 35.0%
! GAGGATTTTCTGATGATCAA+GGG + Chr1:87114363-87114382 MsG0180005110.01.T01:CDS 35.0%
!! AGTTTGAAGTACCGGTAAAT+TGG - Chr1:87111235-87111254 None:intergenic 35.0%
!! TGTGTCCTCTTACTTTTACA+TGG + Chr1:87112673-87112692 MsG0180005110.01.T01:intron 35.0%
!! TTTTGTCATTTCACGGTACA+AGG - Chr1:87111258-87111277 None:intergenic 35.0%
AAAGACATTACACCTATGGG+TGG + Chr1:87114315-87114334 MsG0180005110.01.T01:CDS 40.0%
AAGCGTATGAAAGTGATGAG+GGG + Chr1:87112926-87112945 MsG0180005110.01.T01:intron 40.0%
AAGGGAAATCAGATGCACTA+CGG - Chr1:87111743-87111762 None:intergenic 40.0%
AATGTAGTCACCGTTAATGG+TGG + Chr1:87113294-87113313 MsG0180005110.01.T01:intron 40.0%
ACAGTGAAAGAAACTCTAGC+AGG - Chr1:87114013-87114032 None:intergenic 40.0%
ACTTCTTCAGTTTCTCAAGC+TGG - Chr1:87112969-87112988 None:intergenic 40.0%
ATACCAGCCTTAAACCAAGA+AGG - Chr1:87112234-87112253 None:intergenic 40.0%
ATCTGTGCAAACTCAGCATT+TGG + Chr1:87113173-87113192 MsG0180005110.01.T01:intron 40.0%
ATTTACAAGCTCGGCAAAGT+TGG + Chr1:87114085-87114104 MsG0180005110.01.T01:CDS 40.0%
CAGTCCTGTTAACAAAAGGT+TGG - Chr1:87114293-87114312 None:intergenic 40.0%
CTAACTGCCTTCTTGGTTTA+AGG + Chr1:87112224-87112243 MsG0180005110.01.T01:intron 40.0%
CTGTTCTCAAGCACTTTGAT+TGG - Chr1:87111770-87111789 None:intergenic 40.0%
CTTCTTCAGTTTCTCAAGCT+GGG - Chr1:87112968-87112987 None:intergenic 40.0%
GAATGATACTTACTGGATCC+TGG - Chr1:87112285-87112304 None:intergenic 40.0%
GACAAGAAGTCTGCTTAAGA+AGG - Chr1:87114434-87114453 None:intergenic 40.0%
GTAAGAGGACACAACATTGT+TGG - Chr1:87112666-87112685 None:intergenic 40.0%
TATCAGCAGTAACTACTACG+TGG + Chr1:87111141-87111160 MsG0180005110.01.T01:CDS 40.0%
TATTGATGCTGTGTTCCAGA+AGG + Chr1:87113853-87113872 MsG0180005110.01.T01:intron 40.0%
TCACCTATCGAATTCAGTCT+GGG - Chr1:87114128-87114147 None:intergenic 40.0%
TCAGAACATTGAACACTGTG+TGG + Chr1:87112879-87112898 MsG0180005110.01.T01:CDS 40.0%
TCTGTGCAAACTCAGCATTT+GGG + Chr1:87113174-87113193 MsG0180005110.01.T01:intron 40.0%
TCTTCCTTGTAAGACTCACA+GGG + Chr1:87113952-87113971 MsG0180005110.01.T01:CDS 40.0%
TGCCACATAACCACCATTAA+CGG - Chr1:87113307-87113326 None:intergenic 40.0%
TTGAGGAAGGTTGCATGTAT+TGG + Chr1:87113977-87113996 MsG0180005110.01.T01:CDS 40.0%
TTGCTTAGGAAGCTGAGATT+TGG - Chr1:87111557-87111576 None:intergenic 40.0%
! ATGGAGATTCAAGTTAACGG+TGG + Chr1:87113777-87113796 MsG0180005110.01.T01:CDS 40.0%
! CACTAATTAGGTGCTATGAG+CGG + Chr1:87113467-87113486 MsG0180005110.01.T01:intron 40.0%
! GATTTTCTGATGATCAAGGG+AGG + Chr1:87114366-87114385 MsG0180005110.01.T01:CDS 40.0%
! GGAGGATTTTCTGATGATCA+AGG + Chr1:87114362-87114381 MsG0180005110.01.T01:CDS 40.0%
! GTACAAGGAGTTTGAAGTAC+CGG - Chr1:87111243-87111262 None:intergenic 40.0%
! TTTGGGATCATCGGAGAATT+TGG + Chr1:87111668-87111687 MsG0180005110.01.T01:intron 40.0%
!! AGATTTGGTGGACATTCTCT+TGG - Chr1:87111542-87111561 None:intergenic 40.0%
!! GAGTAGCTCTGATTGTTCAT+TGG + Chr1:87114146-87114165 MsG0180005110.01.T01:intron 40.0%
!! TTCAATGTTCTGAGACCACT+GGG - Chr1:87112872-87112891 None:intergenic 40.0%
!! TTCTGGAACACAGCATCAAT+AGG - Chr1:87113854-87113873 None:intergenic 40.0%
AAACCCCTGTGAGTCTTACA+AGG - Chr1:87113959-87113978 None:intergenic 45.0%
AACCAAGAAGGCAGTTAGCT+TGG - Chr1:87112222-87112241 None:intergenic 45.0%
AAGTCTGCTTAAGAAGGGAC+TGG - Chr1:87114428-87114447 None:intergenic 45.0%
ACAACACCGACAACAACCAT+GGG - Chr1:87112833-87112852 None:intergenic 45.0%
ACACTACGGTATTGTCAAGG+AGG + Chr1:87114344-87114363 MsG0180005110.01.T01:CDS 45.0%
ACCAATGGTAAGGGTTACGA+AGG + Chr1:87113902-87113921 MsG0180005110.01.T01:CDS 45.0%
ACCTTCGTAACCCTTACCAT+TGG - Chr1:87113906-87113925 None:intergenic 45.0%
AGATTCAGGAGTTGTAGGCA+AGG - Chr1:87111207-87111226 None:intergenic 45.0%
AGGGAAATCAGATGCACTAC+GGG - Chr1:87111742-87111761 None:intergenic 45.0%
AGGGGTCTCAATGATTGTCT+CGG - Chr1:87112810-87112829 None:intergenic 45.0%
AGGTTGCATGTATTGGAGCT+TGG + Chr1:87113984-87114003 MsG0180005110.01.T01:CDS 45.0%
AGTGCATCTGATTTCCCTTC+AGG + Chr1:87111744-87111763 MsG0180005110.01.T01:intron 45.0%
ATTGGAGGGACCAATGGTAA+GGG + Chr1:87113893-87113912 MsG0180005110.01.T01:CDS 45.0%
CACCGTTAATGGTGGTTATG+TGG + Chr1:87113302-87113321 MsG0180005110.01.T01:intron 45.0%
CCTTGACAATACCGTAGTGT+GGG - Chr1:87114344-87114363 None:intergenic 45.0%
CTAGAGTCAGAAATCTCGTG+TGG - Chr1:87113218-87113237 None:intergenic 45.0%
CTCACCTATCGAATTCAGTC+TGG - Chr1:87114129-87114148 None:intergenic 45.0%
CTCGACTACTCAGACAATGT+GGG - Chr1:87112259-87112278 None:intergenic 45.0%
CTTAGGAAGCTGAGATTTGG+TGG - Chr1:87111554-87111573 None:intergenic 45.0%
CTTCCTTGTAAGACTCACAG+GGG + Chr1:87113953-87113972 MsG0180005110.01.T01:CDS 45.0%
GACTTCTTGTCTTGCTCTTG+AGG + Chr1:87114443-87114462 MsG0180005110.01.T01:CDS 45.0%
GCAGAAGAAAGCCCATATCA+TGG + Chr1:87113758-87113777 MsG0180005110.01.T01:CDS 45.0%
GGTTATGTGAAAACTCCCAG+TGG + Chr1:87112854-87112873 MsG0180005110.01.T01:CDS 45.0%
GTCTTCCTTGTAAGACTCAC+AGG + Chr1:87113951-87113970 MsG0180005110.01.T01:CDS 45.0%
TAGGAGTCAGAAATCTCGTG+TGG - Chr1:87113498-87113517 None:intergenic 45.0%
TCCTTGACAATACCGTAGTG+TGG - Chr1:87114345-87114364 None:intergenic 45.0%
TCTCGACTACTCAGACAATG+TGG - Chr1:87112260-87112279 None:intergenic 45.0%
TGCAACTGTTATCCGTGTGT+TGG + Chr1:87112990-87113009 MsG0180005110.01.T01:intron 45.0%
TGTTCAGCTCAGTTCTGTGA+TGG - Chr1:87114060-87114079 None:intergenic 45.0%
! ACCAAGAAGGCAGTTAGCTT+GGG - Chr1:87112221-87112240 None:intergenic 45.0%
!! AAGCACTTTGATTGGCCTGA+AGG - Chr1:87111762-87111781 None:intergenic 45.0%
!! AGCACTTTGATTGGCCTGAA+GGG - Chr1:87111761-87111780 None:intergenic 45.0%
!! ATGGTTGTTGTCGGTGTTGT+GGG + Chr1:87112833-87112852 MsG0180005110.01.T01:CDS 45.0%
!! GTTCAATGTTCTGAGACCAC+TGG - Chr1:87112873-87112892 None:intergenic 45.0%
!!! ATTTTTTTTTTTTGTTTAAT+TGG + Chr1:87112112-87112131 MsG0180005110.01.T01:intron 5.0%
AAGACGAGTAACACCCCAAC+GGG - Chr1:87113936-87113955 None:intergenic 50.0%
AATCATTGAGACCCCTCCCA+TGG + Chr1:87112814-87112833 MsG0180005110.01.T01:CDS 50.0%
ACCCAAGCTAACTGCCTTCT+TGG + Chr1:87112217-87112236 MsG0180005110.01.T01:intron 50.0%
ACGCCCAGACTGAATTCGAT+AGG + Chr1:87114122-87114141 MsG0180005110.01.T01:CDS 50.0%
ATGATCAAGGGAGGCTGTGT+TGG + Chr1:87114375-87114394 MsG0180005110.01.T01:CDS 50.0%
ATGGGTGGTTTCCCACACTA+CGG + Chr1:87114330-87114349 MsG0180005110.01.T01:CDS 50.0%
CACAACACCGACAACAACCA+TGG - Chr1:87112834-87112853 None:intergenic 50.0%
CATTGGAGGGACCAATGGTA+AGG + Chr1:87113892-87113911 MsG0180005110.01.T01:CDS 50.0%
CCCACACTACGGTATTGTCA+AGG + Chr1:87114341-87114360 MsG0180005110.01.T01:CDS 50.0%
CCTGAATGTGAGCCAACACA+CGG - Chr1:87113005-87113024 None:intergenic 50.0%
GAAGACGAGTAACACCCCAA+CGG - Chr1:87113937-87113956 None:intergenic 50.0%
GAATTCAGTCTGGGCGTCAT+GGG - Chr1:87114119-87114138 None:intergenic 50.0%
GACATCATTGGAGGGACCAA+TGG + Chr1:87113887-87113906 MsG0180005110.01.T01:CDS 50.0%
GCTTGGTTGGGTCATCCTTA+GGG - Chr1:87112196-87112215 None:intergenic 50.0%
GGGGTCTCAATGATTGTCTC+GGG - Chr1:87112809-87112828 None:intergenic 50.0%
GTAAGACTCACAGGGGTTTG+AGG + Chr1:87113960-87113979 MsG0180005110.01.T01:CDS 50.0%
GTGGACTCAGCATTTGAGTG+AGG + Chr1:87112898-87112917 MsG0180005110.01.T01:CDS 50.0%
TAGTGTGGGAAACCACCCAT+AGG - Chr1:87114330-87114349 None:intergenic 50.0%
TCTTTCACTGTTGCCAGAGC+TGG + Chr1:87114022-87114041 MsG0180005110.01.T01:CDS 50.0%
TGCAGTGAAGGCTTTCCCTA+AGG + Chr1:87112178-87112197 MsG0180005110.01.T01:intron 50.0%
TGGTACAATCGCACAGAAGG+TGG + Chr1:87113797-87113816 MsG0180005110.01.T01:intron 50.0%
! AAGAAGGCAGTTAGCTTGGG+TGG - Chr1:87112218-87112237 None:intergenic 50.0%
! CAGCATTTGGGTGCTATGAG+CGG + Chr1:87113186-87113205 MsG0180005110.01.T01:intron 50.0%
! CTGCCTTCTTGGTTTAAGGC+TGG + Chr1:87112228-87112247 MsG0180005110.01.T01:intron 50.0%
! CTGTGTTGGTCCCAAGAAGA+TGG + Chr1:87114389-87114408 MsG0180005110.01.T01:CDS 50.0%
! GCATGGGAGTTTGGGATCAT+CGG + Chr1:87111659-87111678 MsG0180005110.01.T01:intron 50.0%
!! ACGAAGGTGTTGTTACCCGT+TGG + Chr1:87113918-87113937 MsG0180005110.01.T01:CDS 50.0%
!! CATGGTTGTTGTCGGTGTTG+TGG + Chr1:87112832-87112851 MsG0180005110.01.T01:CDS 50.0%
!! CGAAGGTGTTGTTACCCGTT+GGG + Chr1:87113919-87113938 MsG0180005110.01.T01:CDS 50.0%
!! GAAGGTGTTGTTACCCGTTG+GGG + Chr1:87113920-87113939 MsG0180005110.01.T01:CDS 50.0%
!! TCTGCAAAGCATCGTCGCAT+GGG + Chr1:87111643-87111662 MsG0180005110.01.T01:intron 50.0%
!! TTAGCTTGGGTGGCTTGGTT+GGG - Chr1:87112208-87112227 None:intergenic 50.0%
!! TTCTGCAAAGCATCGTCGCA+TGG + Chr1:87111642-87111661 MsG0180005110.01.T01:intron 50.0%
ACACCGACAACAACCATGGG+AGG - Chr1:87112830-87112849 None:intergenic 55.0%
ACCCCTCCCATGGTTGTTGT+CGG + Chr1:87112824-87112843 MsG0180005110.01.T01:CDS 55.0%
ACCGACAACAACCATGGGAG+GGG - Chr1:87112828-87112847 None:intergenic 55.0%
AGCATCGTCGCATGGGAGTT+TGG + Chr1:87111650-87111669 MsG0180005110.01.T01:intron 55.0%
CAAGCTCGGCAAAGTTGGAG+AGG + Chr1:87114090-87114109 MsG0180005110.01.T01:CDS 55.0%
CACCGACAACAACCATGGGA+GGG - Chr1:87112829-87112848 None:intergenic 55.0%
CGAATTCAGTCTGGGCGTCA+TGG - Chr1:87114120-87114139 None:intergenic 55.0%
CGGTGGTACAATCGCACAGA+AGG + Chr1:87113794-87113813 MsG0180005110.01.T01:CDS 55.0%
GACTCACAGGGGTTTGAGGA+AGG + Chr1:87113964-87113983 MsG0180005110.01.T01:CDS 55.0%
GCATCGTCGCATGGGAGTTT+GGG + Chr1:87111651-87111670 MsG0180005110.01.T01:intron 55.0%
GTATCCGTTCTGACCAGCTC+TGG - Chr1:87114038-87114057 None:intergenic 55.0%
GTCTGAGTAGTCGAGAAGCC+AGG + Chr1:87112264-87112283 MsG0180005110.01.T01:intron 55.0%
GTTGCCAGAGCTGGTCAGAA+CGG + Chr1:87114031-87114050 MsG0180005110.01.T01:CDS 55.0%
! GGCTTGGTTGGGTCATCCTT+AGG - Chr1:87112197-87112216 None:intergenic 55.0%
!! CCGTGTGTTGGCTCACATTC+AGG + Chr1:87113002-87113021 MsG0180005110.01.T01:intron 55.0%
!! GTTAGCTTGGGTGGCTTGGT+TGG - Chr1:87112209-87112228 None:intergenic 55.0%
! GGCAGTTAGCTTGGGTGGCT+TGG - Chr1:87112213-87112232 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 87111131 87114590 87111131 ID=MsG0180005110.01;Name=MsG0180005110.01
Chr1 mRNA 87111131 87114590 87111131 ID=MsG0180005110.01.T01;Parent=MsG0180005110.01;Name=MsG0180005110.01.T01;_AED=0.34;_eAED=0.35;_QI=0|0|0|0.8|1|1|5|0|338
Chr1 exon 87111131 87111287 87111131 ID=MsG0180005110.01.T01:exon:16901;Parent=MsG0180005110.01.T01
Chr1 exon 87112780 87112937 87112780 ID=MsG0180005110.01.T01:exon:16902;Parent=MsG0180005110.01.T01
Chr1 exon 87113735 87113815 87113735 ID=MsG0180005110.01.T01:exon:16903;Parent=MsG0180005110.01.T01
Chr1 exon 87113872 87114143 87113872 ID=MsG0180005110.01.T01:exon:16904;Parent=MsG0180005110.01.T01
Chr1 exon 87114242 87114590 87114242 ID=MsG0180005110.01.T01:exon:16905;Parent=MsG0180005110.01.T01
Chr1 CDS 87111131 87111287 87111131 ID=MsG0180005110.01.T01:cds;Parent=MsG0180005110.01.T01
Chr1 CDS 87112780 87112937 87112780 ID=MsG0180005110.01.T01:cds;Parent=MsG0180005110.01.T01
Chr1 CDS 87113735 87113815 87113735 ID=MsG0180005110.01.T01:cds;Parent=MsG0180005110.01.T01
Chr1 CDS 87113872 87114143 87113872 ID=MsG0180005110.01.T01:cds;Parent=MsG0180005110.01.T01
Chr1 CDS 87114242 87114590 87114242 ID=MsG0180005110.01.T01:cds;Parent=MsG0180005110.01.T01
Gene Sequence

>MsG0180005110.01.T01

ATGGCGGAGTTATCAGCAGTAACTACTACGTGGCCATTTGATATAATCAAACACAAGGAATTTGGGAAGCATGCCTTGCCTACAACTCCTGAATCTAATATCCAATTTACCGGTACTTCAAACTCCTTGTACCGTGAAATGACAAAATCTCTTGTCCAGCTTCACAAGAAGGAGACTTGTGAGCCCGAGACAATCATTGAGACCCCTCCCATGGTTGTTGTCGGTGTTGTGGGTTATGTGAAAACTCCCAGTGGTCTCAGAACATTGAACACTGTGTGGACTCAGCATTTGAGTGAGGAAATTAAGCGTATGAAATTTAGGAAAATGAAGGGTTTGAAGCAGAAGAAAGCCCATATCATGGAGATTCAAGTTAACGGTGGTACAATCGCACAGAAGAAGGATGAAATGATTGACATCATTGGAGGGACCAATGGTAAGGGTTACGAAGGTGTTGTTACCCGTTGGGGTGTTACTCGTCTTCCTTGTAAGACTCACAGGGGTTTGAGGAAGGTTGCATGTATTGGAGCTTGGCATCCTGCTAGAGTTTCTTTCACTGTTGCCAGAGCTGGTCAGAACGGATACCATCACAGAACTGAGCTGAACAAGAAAATTTACAAGCTCGGCAAAGTTGGAGAGGAGACCCATGACGCCCAGACTGAATTCGATAGTTTAGGAAAAAATGTTGATTATTCTATCGAGATGAAAACCAAACTTATCCAACCTTTTGTTAACAGGACTGAGAAAGACATTACACCTATGGGTGGTTTCCCACACTACGGTATTGTCAAGGAGGATTTTCTGATGATCAAGGGAGGCTGTGTTGGTCCCAAGAAGATGGTTATTACTCTGCGCCAGTCCCTTCTTAAGCAGACTTCTTGTCTTGCTCTTGAGGATATCAAACTCATGTTTATCGATACCTCTTCCAAGCTTAATTATGGTAGTAGTTTTGAAATGTTGGGAACTTGTTGTTCATGTTTTGTTACCATCTGTTTTAAACTATGTACTGTCTCCGTCTGA
Protein sequence

>MsG0180005110.01.T01

MAELSAVTTTWPFDIIKHKEFGKHALPTTPESNIQFTGTSNSLYREMTKSLVQLHKKETCEPETIIETPPMVVVGVVGYVKTPSGLRTLNTVWTQHLSEEIKRMKFRKMKGLKQKKAHIMEIQVNGGTIAQKKDEMIDIIGGTNGKGYEGVVTRWGVTRLPCKTHRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTELNKKIYKLGKVGEETHDAQTEFDSLGKNVDYSIEMKTKLIQPFVNRTEKDITPMGGFPHYGIVKEDFLMIKGGCVGPKKMVITLRQSLLKQTSCLALEDIKLMFIDTSSKLNYGSSFEMLGTCCSCFVTICFKLCTVSV*