Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019892.01.T01 | XP_013443636.1 | 75.475 | 1211 | 109 | 12 | 1 | 1026 | 1 | 1208 | 0 | 1646 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019892.01.T01 | A0A072TKD3 | 75.475 | 1211 | 109 | 12 | 1 | 1026 | 1 | 1208 | 0.0 | 1646 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280008759.01 | MsG0480019892.01 | 0.847245 | 1.306753e-59 | 2.308387e-56 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019892.01.T01 | MTR_0003s0460 | 75.475 | 1211 | 109 | 12 | 1 | 1026 | 1 | 1208 | 0.0 | 1646 |
| MsG0480019892.01.T01 | MTR_1g069910 | 67.208 | 924 | 232 | 10 | 2 | 858 | 6 | 925 | 0.0 | 1056 |
| MsG0480019892.01.T01 | MTR_1g069910 | 57.209 | 215 | 38 | 2 | 866 | 1026 | 1002 | 1216 | 1.43e-61 | 229 |
| MsG0480019892.01.T01 | MTR_1g058610 | 61.056 | 303 | 83 | 4 | 302 | 570 | 306 | 607 | 6.63e-101 | 331 |
| MsG0480019892.01.T01 | MTR_1g059520 | 52.312 | 346 | 81 | 6 | 574 | 886 | 1 | 295 | 1.32e-67 | 229 |
| MsG0480019892.01.T01 | MTR_8g072890 | 23.775 | 959 | 601 | 28 | 6 | 873 | 16 | 935 | 1.51e-34 | 144 |
| MsG0480019892.01.T01 | MTR_8g037325 | 24.086 | 930 | 571 | 33 | 6 | 852 | 3 | 880 | 1.27e-32 | 137 |
| MsG0480019892.01.T01 | MTR_7g091420 | 32.278 | 158 | 99 | 2 | 6 | 155 | 11 | 168 | 1.16e-19 | 93.2 |
| MsG0480019892.01.T01 | MTR_1g051825 | 23.670 | 752 | 445 | 28 | 6 | 678 | 27 | 728 | 3.39e-18 | 90.9 |
| MsG0480019892.01.T01 | MTR_1g051825 | 23.670 | 752 | 445 | 28 | 6 | 678 | 3 | 704 | 3.57e-18 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019892.01.T01 | AT3G54670 | 54.555 | 922 | 349 | 9 | 4 | 858 | 9 | 927 | 0.0 | 883 |
| MsG0480019892.01.T01 | AT3G54670 | 47.082 | 257 | 75 | 4 | 824 | 1026 | 969 | 1218 | 2.35e-54 | 207 |
| MsG0480019892.01.T01 | AT3G47460 | 23.693 | 899 | 572 | 29 | 6 | 833 | 3 | 858 | 4.65e-35 | 145 |
| MsG0480019892.01.T01 | AT3G47460 | 23.614 | 902 | 572 | 29 | 6 | 833 | 3 | 861 | 2.15e-34 | 143 |
| MsG0480019892.01.T01 | AT5G62410 | 24.459 | 924 | 537 | 34 | 6 | 833 | 3 | 861 | 1.85e-31 | 134 |
| MsG0480019892.01.T01 | AT5G48600 | 27.221 | 349 | 209 | 8 | 6 | 321 | 26 | 362 | 2.05e-23 | 108 |
| MsG0480019892.01.T01 | AT5G48600 | 27.221 | 349 | 209 | 8 | 6 | 321 | 26 | 362 | 2.21e-23 | 107 |
Find 180 sgRNAs with CRISPR-Local
Find 268 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAAGCTTTCAAGGTGT+TGG | 0.472045 | 4:-29064445 | MsG0480019892.01.T01:CDS |
| AAAAGAGATGAATCAGTTGA+AGG | 0.483918 | 4:-29062612 | MsG0480019892.01.T01:CDS |
| AAAAGGCAGAGGAACATCTT+CGG | 0.545741 | 4:-29064570 | MsG0480019892.01.T01:CDS |
| AAAAGTAAAGTAAGTTCCTT+TGG | 0.351545 | 4:+29064739 | None:intergenic |
| AAAATCTTGTGAAGGAGAGA+GGG | 0.602787 | 4:-29061515 | MsG0480019892.01.T01:CDS |
| AAAGAGAATTGCCCTCTCCA+AGG | 0.611260 | 4:+29063395 | None:intergenic |
| AAAGAGATGAATCAGTTGAA+GGG | 0.537967 | 4:-29062611 | MsG0480019892.01.T01:CDS |
| AAAGATCAATCATGCGACCA+TGG | 0.625528 | 4:+29063661 | None:intergenic |
| AAAGGGGCTACAGAACAAAG+AGG | 0.694287 | 4:-29062666 | MsG0480019892.01.T01:CDS |
| AAAGTGGAAATCCAAGTTAG+AGG | 0.483116 | 4:-29062579 | MsG0480019892.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAGAGATGAATCAGTTGA+AGG | - | Chr4:29063937-29063956 | MsG0480019892.01.T01:CDS | 30.0% | |
| AAAGAGATGAATCAGTTGAA+GGG | - | Chr4:29063938-29063957 | MsG0480019892.01.T01:CDS | 30.0% | |
| AGATTATACTTCTTCTGTGT+TGG | + | Chr4:29062919-29062938 | None:intergenic | 30.0% | |
| ATTCGTTCAAAATCTTGTGA+AGG | - | Chr4:29065026-29065045 | MsG0480019892.01.T01:CDS | 30.0% | |
| CATTAATTTACCCATAGCAA+CGG | + | Chr4:29062950-29062969 | None:intergenic | 30.0% | |
| CTACTTCAGACACTTTAATA+TGG | + | Chr4:29063431-29063450 | None:intergenic | 30.0% | |
| GGAAGATTCTTCAAATTTCT+TGG | + | Chr4:29063349-29063368 | None:intergenic | 30.0% | |
| GGCTTATAACTGAAAAGATA+AGG | + | Chr4:29064933-29064952 | None:intergenic | 30.0% | |
| GTAGAATAAAAGAGGAAGTT+AGG | - | Chr4:29062485-29062504 | MsG0480019892.01.T01:CDS | 30.0% | |
| GTATGAAGAAAATCAACTCA+AGG | - | Chr4:29063715-29063734 | MsG0480019892.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 29061351 | 29065220 | 29061351 | ID=MsG0480019892.01;Name=MsG0480019892.01 |
| Chr4 | mRNA | 29061351 | 29065220 | 29061351 | ID=MsG0480019892.01.T01;Parent=MsG0480019892.01;Name=MsG0480019892.01.T01;_AED=0.17;_eAED=0.17;_QI=0|0|0|1|1|1|5|0|1026 |
| Chr4 | exon | 29064233 | 29065220 | 29064233 | ID=MsG0480019892.01.T01:exon:9622;Parent=MsG0480019892.01.T01 |
| Chr4 | exon | 29062979 | 29064146 | 29062979 | ID=MsG0480019892.01.T01:exon:9621;Parent=MsG0480019892.01.T01 |
| Chr4 | exon | 29062439 | 29062877 | 29062439 | ID=MsG0480019892.01.T01:exon:9620;Parent=MsG0480019892.01.T01 |
| Chr4 | exon | 29062026 | 29062234 | 29062026 | ID=MsG0480019892.01.T01:exon:9619;Parent=MsG0480019892.01.T01 |
| Chr4 | exon | 29061351 | 29061627 | 29061351 | ID=MsG0480019892.01.T01:exon:9618;Parent=MsG0480019892.01.T01 |
| Chr4 | CDS | 29064233 | 29065220 | 29064233 | ID=MsG0480019892.01.T01:cds;Parent=MsG0480019892.01.T01 |
| Chr4 | CDS | 29062979 | 29064146 | 29062979 | ID=MsG0480019892.01.T01:cds;Parent=MsG0480019892.01.T01 |
| Chr4 | CDS | 29062439 | 29062877 | 29062439 | ID=MsG0480019892.01.T01:cds;Parent=MsG0480019892.01.T01 |
| Chr4 | CDS | 29062026 | 29062234 | 29062026 | ID=MsG0480019892.01.T01:cds;Parent=MsG0480019892.01.T01 |
| Chr4 | CDS | 29061351 | 29061627 | 29061351 | ID=MsG0480019892.01.T01:cds;Parent=MsG0480019892.01.T01 |
| Gene Sequence |
>MsG0480019892.01.T01 ATGTCACTGGGGAAAATTCACCGTCTCGAGATACAGAATTTCAAGTCATACAAAGGTTCCCAAATCATCGGTCCCTTCTCCGGCTTCACAGCCATCATCGGCCCTAATGGCGCTGGTAAATCAAACCTAATGGACGCTATCTGCTTTGTTCTTGGCGTTAGAACCATTCACCTCCGTGGTTCTCAATTAAAGGAACTTATCTACACTGCCTTCGATGACAGCGAGAAACAGAAGAATGAACGTTCTGCCTTGGTTCGCTTGGTTTATCAACTCGCAGACAAATCTGAGATTCAATTTGCAAGAACCATCACTGCCACAGGCGCAAGTCAGTACTTCATCGACGGTAATGTGGTTACCTGGGATGTCTACAATGCTAAACTCATATCCCTTGACATTCTTGTCAAAGCTCGCAATTTCTTGGTCTTCCAGGGTGATGTTGAATCCATTGCATCGAAAAATCCAAAGGAACTTACTTTACTTTTCGAGCAGATATCTGGCTCGGATCAATTCAAGAGAGAGTACGAGAAGTTGGAAGAAGACAAAAATTCTGCTGAGGAGAAATTGAAACTTGTTTATCAGAAGAAAAAGACTATTTTGATGGAGAAAAAGAAGAAGAAAGAACAAAAGAAAAAGGCAGAGGAACATCTTCGGTTGCAAGACGAATTGAAATCTATGAAAAAGGAACATTTCTTGTGGAAGTTGTTCAATATAGAGAATGATTTTGCTAAAACCACTGAGGAACTTGAAGTTGATAAAACAAGCTTTCAAGGTGTTGGTAAAGAGGTAGAAAAATTTGAACATGAAGCCAATGAAAAGGAAAAGGAGCTAACCAGATGTGTTGAAAAGATTAAGCTACTCGAGAAGAAAATAACTGAGATAAGCAACAAACTTGATGACAAGACTCGACCCGATGTTATCAAGTTAAAGGAGGAAATATCTCGTATCAATTTGAAAATTGAAAAAACGAAGAAGGAGCTCAGCAAAAAAGGAAAAGGGTCGAAATGTTGGGTCGAAAAGGGTCGAGATGGCAATGACTTGGAAGAATATAGTAGAATAAAAGAGGAAGTTAGGATAAAAACTGCAAATCTTAGAGAGAAAAAAGAATTTCTTGATGGGCAGCAACGTGCTGATAGTGAAGCTCAAACTAATTTGGAAGAAACTCTTCAACAATTAAAAAACCGGGAGTCAGAGCTGGATTCAAAGGAAAAACAGATGCGAGAAAGGCTTGATAGTTTTGCAAAGAATAAGGATGACCTGGAAAACGCGAAGAAAGAGCTGTGTGTGTTGCAGGAAAAACAAAGAGATTTCAAGCGAGAATATGATGTCTTGAAGAAAAAAATTGGTGATGTAGTAAATGAACTACATGAATTGAAATCTGATAGATATGAAAACGAGAGAGATGCTAAGTTTGCTGATACTGTGGCGACTCTAAAACGCTTGTTTCAAGGAGTCCATGGTCGCATGATTGATCTTTGTAGACCAACACAGAAGAAGTATAATCTAGCCGTTACCGTTGCTATGGGTAAATTAATGGATGCAATTGTTGTTGAGGATGAAAATACTGCGAAGGAATGCATCAGGCATTTAAAAGACCTGCGGATTCCTCCCCAGACATTTATTCCTCTACGGTCTATTTGTGTGCAGCTAATAATCGAAAGATTACGCTCATTAGGGGGTACCACAAAATTGGTTTTTGATGTGATTCAGTTTGATCCTTCCTTGGAGAGGGCAATTCTCTTTGCTGTTGGGAACACTCTTGTTTGTGAAGATCTTGAGGAGGCAAAAATTTTAAGCTGGACCGGTGAGAGGTTAAAAGTTGTAACTGTGGACGGAATTCTGCTGACACAATCTGGCACAATGACTGGTGGCACTAGTGGTGGAATGGAAGCAAGGTCAAAAAAGTGGGATGCCAAGAAATTTGAAGAATCTTCCCAGAAGAAAGAAAAGTATGAATCAAAGTTGGAAGAGCTAGGATCAATTAGAGATATGCACCATATTAAAGTGTCTGAAGTAGAGGAAAAAATTAGCGAACTCAAGAAGAAAGCTCAATATGCTGAGATTGAAAAGCAAGGCATTGAAAAGAAGCTTTCAACATTGAGCGATGAAAAGGGGACTATCAAAAAAGAAATTGAACGAGTTAGTCCAGAACTGATCAATTGGGTTGCAAACATTCGCGAGTATGAAGAAAATCAACTCAAGGCTTCTCAAAATGCAGCAGAGGAGAGACTGAACCTGAGTAGCCAACTTTCAAAATTAAAATATCAGTTGGAGTATGAGCAAAACCGGGACATGAGTTCTCGAATTCAAGAATTGGAATCTTCGCTGAGTGCTTTAAAGAATGACTTAAAGGGGCTACAGAACAAAGAGGCCGAAGCAAAGTTAGCAGCGGAGAATGCTACAAAAGAGATGAATCAGTTGAAGGGTGAATATGAAAAGTGGAAATCCAAGTTAGAGGATTGTGAAAAGGAAATTCAGGAACGGAAGAAGAGGGCTTCTGCAGCCAAAACAAACTTATCCAAACTTAATCATCAGATTAAGTCAAAGGAGGAACGGATTGAGAAGTTAATGGGGAAAAACAGGAAATACAAGAAAAGTGCATTGAAACAGTATGATTCCCTGGTTGAAAAAGAAAGACTTATAACTGAAGAGTTTGAAGCTGTCATGAAAGAAGAGAAGGAAAAAACAGATAGATTCAATGCGGTTAAGCAGGAGAGATATAATTTGTTCATGGGTGCTTTCAACCGTATATCTGGTAACATAGATAAAATCTACAAACAACTTACAAAGAGCAACACACCTTTTTGTTATAAGCCTTCACCATTTTTCATACTTGATGAAGTTGATGCTGCATTGGACAACTTGAATGTTGCGAAAGTTGCTAGCTTTATTCGTTCAAAATCTTGTGAAGGAGAGAGGGCTAATCAAGACACCGATGATGGAAAGGGTTTTCAGATAATTCAAATATCACAGAAAGATCACTTCTATAACAAAGCTGACGTGTTAGTTGGGGTTTGCAGAGACTCTAAAAGTGGCAGTTCGAGAACTCTCTCGCTTGATCTCACTAAATACCGGGAGTCCTAA |
| Protein sequence |
>MsG0480019892.01.T01 MSLGKIHRLEIQNFKSYKGSQIIGPFSGFTAIIGPNGAGKSNLMDAICFVLGVRTIHLRGSQLKELIYTAFDDSEKQKNERSALVRLVYQLADKSEIQFARTITATGASQYFIDGNVVTWDVYNAKLISLDILVKARNFLVFQGDVESIASKNPKELTLLFEQISGSDQFKREYEKLEEDKNSAEEKLKLVYQKKKTILMEKKKKKEQKKKAEEHLRLQDELKSMKKEHFLWKLFNIENDFAKTTEELEVDKTSFQGVGKEVEKFEHEANEKEKELTRCVEKIKLLEKKITEISNKLDDKTRPDVIKLKEEISRINLKIEKTKKELSKKGKGSKCWVEKGRDGNDLEEYSRIKEEVRIKTANLREKKEFLDGQQRADSEAQTNLEETLQQLKNRESELDSKEKQMRERLDSFAKNKDDLENAKKELCVLQEKQRDFKREYDVLKKKIGDVVNELHELKSDRYENERDAKFADTVATLKRLFQGVHGRMIDLCRPTQKKYNLAVTVAMGKLMDAIVVEDENTAKECIRHLKDLRIPPQTFIPLRSICVQLIIERLRSLGGTTKLVFDVIQFDPSLERAILFAVGNTLVCEDLEEAKILSWTGERLKVVTVDGILLTQSGTMTGGTSGGMEARSKKWDAKKFEESSQKKEKYESKLEELGSIRDMHHIKVSEVEEKISELKKKAQYAEIEKQGIEKKLSTLSDEKGTIKKEIERVSPELINWVANIREYEENQLKASQNAAEERLNLSSQLSKLKYQLEYEQNRDMSSRIQELESSLSALKNDLKGLQNKEAEAKLAAENATKEMNQLKGEYEKWKSKLEDCEKEIQERKKRASAAKTNLSKLNHQIKSKEERIEKLMGKNRKYKKSALKQYDSLVEKERLITEEFEAVMKEEKEKTDRFNAVKQERYNLFMGAFNRISGNIDKIYKQLTKSNTPFCYKPSPFFILDEVDAALDNLNVAKVASFIRSKSCEGERANQDTDDGKGFQIIQISQKDHFYNKADVLVGVCRDSKSGSSRTLSLDLTKYRES* |
