Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019985.01.T01 | XP_039689860.1 | 96.237 | 186 | 4 | 1 | 1 | 183 | 1 | 186 | 8.78E-129 | 369 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019985.01.T01 | Q9M8T3 | 59.777 | 179 | 65 | 1 | 11 | 182 | 5 | 183 | 6.18E-69 | 210 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019985.01.T01 | A0A396I8U0 | 96.237 | 186 | 4 | 1 | 1 | 183 | 1 | 186 | 4.19e-129 | 369 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480019985.01 | MsG0480022491.01 | 0.814398 | 1.572629e-51 | 1.054115e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019985.01.T01 | MTR_5g430420 | 44.578 | 166 | 85 | 2 | 13 | 177 | 2 | 161 | 1.24e-43 | 143 |
| MsG0480019985.01.T01 | MTR_5g030280 | 44.578 | 166 | 85 | 2 | 13 | 177 | 2 | 161 | 1.24e-43 | 143 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019985.01.T01 | AT3G02910 | 59.777 | 179 | 65 | 1 | 11 | 182 | 5 | 183 | 6.30e-70 | 210 |
| MsG0480019985.01.T01 | AT5G46720 | 50.000 | 170 | 85 | 0 | 13 | 182 | 2 | 171 | 1.46e-50 | 161 |
Find 72 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTTCCTCCTCAACATTCC+CGG | 0.280602 | 4:-31194860 | MsG0480019985.01.T01:CDS |
| ATGTTCCGTAGGTGAATATC+AGG | 0.291969 | 4:+31195005 | None:intergenic |
| CCTCCTCAACATTCCCGGTT+CGG | 0.294032 | 4:-31194855 | MsG0480019985.01.T01:CDS |
| CTCCTCAACATTCCCGGTTC+GGG | 0.301032 | 4:-31194854 | MsG0480019985.01.T01:CDS |
| TATCAGGGCGGCGGCGGCGG+CGG | 0.325520 | 4:+31195021 | None:intergenic |
| AAGCGTCGCCGGTGAGGATT+AGG | 0.331566 | 4:+31194945 | None:intergenic |
| TCAGCGGGAACGACCTTGAT+AGG | 0.341709 | 4:+31194734 | None:intergenic |
| AAAAGGTTTGAAGTGTTATA+CGG | 0.367755 | 4:-31194618 | MsG0480019985.01.T01:CDS |
| GGTTCGGGTCAACCCGTTTA+CGG | 0.373409 | 4:-31194839 | MsG0480019985.01.T01:CDS |
| TCTCCGAACACGGTCTTTCC+CGG | 0.379160 | 4:-31194802 | MsG0480019985.01.T01:CDS |
| TTCCTTCGAGTTCGTCCATC+CGG | 0.380538 | 4:+31194783 | None:intergenic |
| GGAGTAGAGTTCGCCGTAAA+CGG | 0.390773 | 4:+31194826 | None:intergenic |
| GAATATCAGGGCGGCGGCGG+CGG | 0.395114 | 4:+31195018 | None:intergenic |
| GAGTAGAGTTCGCCGTAAAC+GGG | 0.427775 | 4:+31194827 | None:intergenic |
| ACCGGCGACGCTTCTTTCAT+CGG | 0.439535 | 4:-31194935 | MsG0480019985.01.T01:CDS |
| TATACGGAGAAAGAAACAAT+TGG | 0.441550 | 4:-31194602 | MsG0480019985.01.T01:CDS |
| CATCCGGGAAAGACCGTGTT+CGG | 0.444092 | 4:+31194799 | None:intergenic |
| GTCTTGGAGAAGAGGGTGGT+TGG | 0.446887 | 4:+31194967 | None:intergenic |
| AGGAGGAAAGGAACGCGGTA+GGG | 0.453658 | 4:+31194872 | None:intergenic |
| GGTATGTGAAACGTAAAGAT+AGG | 0.454065 | 4:-31194580 | MsG0480019985.01.T01:CDS |
| CTCAATTACCCTCTCGTATG+TGG | 0.458676 | 4:-31194896 | MsG0480019985.01.T01:CDS |
| GTAAACGGGTTGACCCGAAC+CGG | 0.459975 | 4:+31194841 | None:intergenic |
| CGCCGGTGAGGATTAGGTCT+TGG | 0.460073 | 4:+31194951 | None:intergenic |
| GGCGGCGGCGGTGGTGGTAA+CGG | 0.469528 | 4:+31195036 | None:intergenic |
| CTCTACTCCGTCTCCGAACA+CGG | 0.492946 | 4:-31194812 | MsG0480019985.01.T01:CDS |
| CTACGAGCGTCTTCCTATCA+AGG | 0.499297 | 4:-31194747 | MsG0480019985.01.T01:CDS |
| AGGATTAGGTCTTGGAGAAG+AGG | 0.501398 | 4:+31194959 | None:intergenic |
| GGAGGAAAGGAACGCGGTAG+GGG | 0.501640 | 4:+31194873 | None:intergenic |
| GGTGAATATCAGGGCGGCGG+CGG | 0.503311 | 4:+31195015 | None:intergenic |
| TCGGCACCATCTTCTTCAGC+GGG | 0.508387 | 4:+31194719 | None:intergenic |
| GGATTAGGTCTTGGAGAAGA+GGG | 0.523117 | 4:+31194960 | None:intergenic |
| TCCTTCGAGTTCGTCCATCC+GGG | 0.535120 | 4:+31194784 | None:intergenic |
| TCCGATGAAAGAAGCGTCGC+CGG | 0.536059 | 4:+31194934 | None:intergenic |
| ATACGGAGAAAGAAACAATT+GGG | 0.536947 | 4:-31194601 | MsG0480019985.01.T01:CDS |
| CACATACGAGAGGGTAATTG+AGG | 0.542933 | 4:+31194897 | None:intergenic |
| TTCGGCACCATCTTCTTCAG+CGG | 0.547069 | 4:+31194718 | None:intergenic |
| GGAAAGACCGTGTTCGGAGA+CGG | 0.548945 | 4:+31194805 | None:intergenic |
| GTCGTTCCCGCTGAAGAAGA+TGG | 0.551863 | 4:-31194725 | MsG0480019985.01.T01:CDS |
| GAGGAGGAAAGGAACGCGGT+AGG | 0.553653 | 4:+31194871 | None:intergenic |
| GCACGTTATCTTCTCCCCTT+CGG | 0.558254 | 4:+31194700 | None:intergenic |
| CCGGGAATGTTGAGGAGGAA+AGG | 0.560533 | 4:+31194860 | None:intergenic |
| CTGAAGAAGATGGTGCCGAA+GGG | 0.565219 | 4:-31194715 | MsG0480019985.01.T01:CDS |
| CTCCAAGACCTAATCCTCAC+CGG | 0.567437 | 4:-31194953 | MsG0480019985.01.T01:CDS |
| GTTTCTCTTTAATGTTCCGT+AGG | 0.568157 | 4:+31194994 | None:intergenic |
| TAAACGGGTTGACCCGAACC+GGG | 0.570822 | 4:+31194842 | None:intergenic |
| GCTGAAGAAGATGGTGCCGA+AGG | 0.571713 | 4:-31194716 | MsG0480019985.01.T01:CDS |
| TGAATAATAATCAATCAGAA+AGG | 0.578539 | 4:+31194519 | None:intergenic |
| TTAGGTCTTGGAGAAGAGGG+TGG | 0.579922 | 4:+31194963 | None:intergenic |
| GTAATTGAGGAGGGTGCGGT+AGG | 0.582397 | 4:+31194910 | None:intergenic |
| CGAACACGGTCTTTCCCGGA+TGG | 0.585584 | 4:-31194798 | MsG0480019985.01.T01:CDS |
| ACGCTCGTAGTGAGCGCGTG+TGG | 0.589717 | 4:+31194760 | None:intergenic |
| CGCGCATGGTAGCTATGCGA+TGG | 0.592586 | 4:-31194663 | MsG0480019985.01.T01:CDS |
| GCCGCCCTGATATTCACCTA+CGG | 0.596124 | 4:-31195010 | MsG0480019985.01.T01:CDS |
| TACGAGAGGGTAATTGAGGA+GGG | 0.597902 | 4:+31194901 | None:intergenic |
| GACCCGAACCGGGAATGTTG+AGG | 0.600079 | 4:+31194852 | None:intergenic |
| GTAGGTGAATATCAGGGCGG+CGG | 0.602911 | 4:+31195012 | None:intergenic |
| GAGGGTAATTGAGGAGGGTG+CGG | 0.604436 | 4:+31194906 | None:intergenic |
| TCAAGAAAAGTCAAATGTTG+AGG | 0.606957 | 4:+31194557 | None:intergenic |
| GCAGAAGCGTATTTCGCGCA+TGG | 0.611765 | 4:-31194677 | MsG0480019985.01.T01:CDS |
| TCCCGGATGGACGAACTCGA+AGG | 0.612609 | 4:-31194785 | MsG0480019985.01.T01:CDS |
| ATGGAGATGTGGAAGAAGAA+TGG | 0.614326 | 4:-31194644 | MsG0480019985.01.T01:CDS |
| AATCCTCCTAAACATGGTAG+CGG | 0.617030 | 4:-31195071 | None:intergenic |
| ATACGAGAGGGTAATTGAGG+AGG | 0.617794 | 4:+31194900 | None:intergenic |
| TGAAAGAAGCGTCGCCGGTG+AGG | 0.622942 | 4:+31194939 | None:intergenic |
| TGAAGAAGATGGTGCCGAAG+GGG | 0.625426 | 4:-31194714 | MsG0480019985.01.T01:CDS |
| GTAGCTATGCGATGGAGATG+TGG | 0.642757 | 4:-31194655 | MsG0480019985.01.T01:CDS |
| CCGAACCGGGAATGTTGAGG+AGG | 0.645176 | 4:+31194855 | None:intergenic |
| CGGTAGGGGCCACATACGAG+AGG | 0.683309 | 4:+31194887 | None:intergenic |
| TGTTCCGTAGGTGAATATCA+GGG | 0.705714 | 4:+31195006 | None:intergenic |
| TGTTGAGGAGGAAAGGAACG+CGG | 0.732139 | 4:+31194867 | None:intergenic |
| GGTAGGGGCCACATACGAGA+GGG | 0.734601 | 4:+31194888 | None:intergenic |
| TCCGTAGGTGAATATCAGGG+CGG | 0.784785 | 4:+31195009 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GGCGGCGGCGGCGGCGGCGG+TGG | + | Chr4:31194563-31194582 | None:intergenic | 100.0% |
| !! | AAAAGGTTTGAAGTGTTATA+CGG | - | Chr4:31194969-31194988 | MsG0480019985.01.T01:CDS | 25.0% |
| ATACGGAGAAAGAAACAATT+GGG | - | Chr4:31194986-31195005 | MsG0480019985.01.T01:CDS | 30.0% | |
| TATACGGAGAAAGAAACAAT+TGG | - | Chr4:31194985-31195004 | MsG0480019985.01.T01:CDS | 30.0% | |
| TCAAGAAAAGTCAAATGTTG+AGG | + | Chr4:31195033-31195052 | None:intergenic | 30.0% | |
| TGGAAGAAGAATGGTAAAAA+AGG | - | Chr4:31194952-31194971 | MsG0480019985.01.T01:CDS | 30.0% | |
| GGTATGTGAAACGTAAAGAT+AGG | - | Chr4:31195007-31195026 | MsG0480019985.01.T01:CDS | 35.0% | |
| GTTTCTCTTTAATGTTCCGT+AGG | + | Chr4:31194596-31194615 | None:intergenic | 35.0% | |
| ATGGAGATGTGGAAGAAGAA+TGG | - | Chr4:31194943-31194962 | MsG0480019985.01.T01:CDS | 40.0% | |
| ATGTTCCGTAGGTGAATATC+AGG | + | Chr4:31194585-31194604 | None:intergenic | 40.0% | |
| TGTTCCGTAGGTGAATATCA+GGG | + | Chr4:31194584-31194603 | None:intergenic | 40.0% | |
| AGGATTAGGTCTTGGAGAAG+AGG | + | Chr4:31194631-31194650 | None:intergenic | 45.0% | |
| ATACGAGAGGGTAATTGAGG+AGG | + | Chr4:31194690-31194709 | None:intergenic | 45.0% | |
| CACATACGAGAGGGTAATTG+AGG | + | Chr4:31194693-31194712 | None:intergenic | 45.0% | |
| CTCAATTACCCTCTCGTATG+TGG | - | Chr4:31194691-31194710 | MsG0480019985.01.T01:CDS | 45.0% | |
| GGATTAGGTCTTGGAGAAGA+GGG | + | Chr4:31194630-31194649 | None:intergenic | 45.0% | |
| TACGAGAGGGTAATTGAGGA+GGG | + | Chr4:31194689-31194708 | None:intergenic | 45.0% | |
| CCTTTCCTCCTCAACATTCC+CGG | - | Chr4:31194727-31194746 | MsG0480019985.01.T01:CDS | 50.0% | |
| CTACGAGCGTCTTCCTATCA+AGG | - | Chr4:31194840-31194859 | MsG0480019985.01.T01:CDS | 50.0% | |
| CTCCAAGACCTAATCCTCAC+CGG | - | Chr4:31194634-31194653 | MsG0480019985.01.T01:CDS | 50.0% | |
| GAGTAGAGTTCGCCGTAAAC+GGG | + | Chr4:31194763-31194782 | None:intergenic | 50.0% | |
| GCACGTTATCTTCTCCCCTT+CGG | + | Chr4:31194890-31194909 | None:intergenic | 50.0% | |
| GGAGTAGAGTTCGCCGTAAA+CGG | + | Chr4:31194764-31194783 | None:intergenic | 50.0% | |
| GTAGCTATGCGATGGAGATG+TGG | - | Chr4:31194932-31194951 | MsG0480019985.01.T01:CDS | 50.0% | |
| TCCGTAGGTGAATATCAGGG+CGG | + | Chr4:31194581-31194600 | None:intergenic | 50.0% | |
| TGTTGAGGAGGAAAGGAACG+CGG | + | Chr4:31194723-31194742 | None:intergenic | 50.0% | |
| TTAGGTCTTGGAGAAGAGGG+TGG | + | Chr4:31194627-31194646 | None:intergenic | 50.0% | |
| TTCGGCACCATCTTCTTCAG+CGG | + | Chr4:31194872-31194891 | None:intergenic | 50.0% | |
| ! | CTGAAGAAGATGGTGCCGAA+GGG | - | Chr4:31194872-31194891 | MsG0480019985.01.T01:CDS | 50.0% |
| ! | TGAAGAAGATGGTGCCGAAG+GGG | - | Chr4:31194873-31194892 | MsG0480019985.01.T01:CDS | 50.0% |
| ! | TTCCTTCGAGTTCGTCCATC+CGG | + | Chr4:31194807-31194826 | None:intergenic | 50.0% |
| ACCGGCGACGCTTCTTTCAT+CGG | - | Chr4:31194652-31194671 | MsG0480019985.01.T01:CDS | 55.0% | |
| AGGAGGAAAGGAACGCGGTA+GGG | + | Chr4:31194718-31194737 | None:intergenic | 55.0% | |
| CATCCGGGAAAGACCGTGTT+CGG | + | Chr4:31194791-31194810 | None:intergenic | 55.0% | |
| CCGGGAATGTTGAGGAGGAA+AGG | + | Chr4:31194730-31194749 | None:intergenic | 55.0% | |
| CCTCCTCAACATTCCCGGTT+CGG | - | Chr4:31194732-31194751 | MsG0480019985.01.T01:CDS | 55.0% | |
| CTCCTCAACATTCCCGGTTC+GGG | - | Chr4:31194733-31194752 | MsG0480019985.01.T01:CDS | 55.0% | |
| CTCTACTCCGTCTCCGAACA+CGG | - | Chr4:31194775-31194794 | MsG0480019985.01.T01:CDS | 55.0% | |
| GAGGGTAATTGAGGAGGGTG+CGG | + | Chr4:31194684-31194703 | None:intergenic | 55.0% | |
| GCCGCCCTGATATTCACCTA+CGG | - | Chr4:31194577-31194596 | MsG0480019985.01.T01:CDS | 55.0% | |
| GGAAAGACCGTGTTCGGAGA+CGG | + | Chr4:31194785-31194804 | None:intergenic | 55.0% | |
| GGTTCGGGTCAACCCGTTTA+CGG | - | Chr4:31194748-31194767 | MsG0480019985.01.T01:CDS | 55.0% | |
| GTAGGTGAATATCAGGGCGG+CGG | + | Chr4:31194578-31194597 | None:intergenic | 55.0% | |
| GTCGTTCCCGCTGAAGAAGA+TGG | - | Chr4:31194862-31194881 | MsG0480019985.01.T01:CDS | 55.0% | |
| GTCTTGGAGAAGAGGGTGGT+TGG | + | Chr4:31194623-31194642 | None:intergenic | 55.0% | |
| TCAGCGGGAACGACCTTGAT+AGG | + | Chr4:31194856-31194875 | None:intergenic | 55.0% | |
| TCGGCACCATCTTCTTCAGC+GGG | + | Chr4:31194871-31194890 | None:intergenic | 55.0% | |
| TCTCCGAACACGGTCTTTCC+CGG | - | Chr4:31194785-31194804 | MsG0480019985.01.T01:CDS | 55.0% | |
| ! | GCAGAAGCGTATTTCGCGCA+TGG | - | Chr4:31194910-31194929 | MsG0480019985.01.T01:CDS | 55.0% |
| ! | GCTGAAGAAGATGGTGCCGA+AGG | - | Chr4:31194871-31194890 | MsG0480019985.01.T01:CDS | 55.0% |
| ! | GTAATTGAGGAGGGTGCGGT+AGG | + | Chr4:31194680-31194699 | None:intergenic | 55.0% |
| ! | TCCTTCGAGTTCGTCCATCC+GGG | + | Chr4:31194806-31194825 | None:intergenic | 55.0% |
| !! | GTAAACGGGTTGACCCGAAC+CGG | + | Chr4:31194749-31194768 | None:intergenic | 55.0% |
| !! | TAAACGGGTTGACCCGAACC+GGG | + | Chr4:31194748-31194767 | None:intergenic | 55.0% |
| !! | TCCGATGAAAGAAGCGTCGC+CGG | + | Chr4:31194656-31194675 | None:intergenic | 55.0% |
| CCGAACCGGGAATGTTGAGG+AGG | + | Chr4:31194735-31194754 | None:intergenic | 60.0% | |
| CGAACACGGTCTTTCCCGGA+TGG | - | Chr4:31194789-31194808 | MsG0480019985.01.T01:CDS | 60.0% | |
| CGCCGGTGAGGATTAGGTCT+TGG | + | Chr4:31194639-31194658 | None:intergenic | 60.0% | |
| CGCGCATGGTAGCTATGCGA+TGG | - | Chr4:31194924-31194943 | MsG0480019985.01.T01:CDS | 60.0% | |
| GACCCGAACCGGGAATGTTG+AGG | + | Chr4:31194738-31194757 | None:intergenic | 60.0% | |
| GAGGAGGAAAGGAACGCGGT+AGG | + | Chr4:31194719-31194738 | None:intergenic | 60.0% | |
| GGAGGAAAGGAACGCGGTAG+GGG | + | Chr4:31194717-31194736 | None:intergenic | 60.0% | |
| GGTAGGGGCCACATACGAGA+GGG | + | Chr4:31194702-31194721 | None:intergenic | 60.0% | |
| TCCCGGATGGACGAACTCGA+AGG | - | Chr4:31194802-31194821 | MsG0480019985.01.T01:CDS | 60.0% | |
| ! | AAGCGTCGCCGGTGAGGATT+AGG | + | Chr4:31194645-31194664 | None:intergenic | 60.0% |
| !! | TGAAAGAAGCGTCGCCGGTG+AGG | + | Chr4:31194651-31194670 | None:intergenic | 60.0% |
| ACGCTCGTAGTGAGCGCGTG+TGG | + | Chr4:31194830-31194849 | None:intergenic | 65.0% | |
| CGGTAGGGGCCACATACGAG+AGG | + | Chr4:31194703-31194722 | None:intergenic | 65.0% | |
| GGTGAATATCAGGGCGGCGG+CGG | + | Chr4:31194575-31194594 | None:intergenic | 65.0% | |
| GAATATCAGGGCGGCGGCGG+CGG | + | Chr4:31194572-31194591 | None:intergenic | 70.0% | |
| ! | GGCGGCGGCGGTGGTGGTAA+CGG | + | Chr4:31194554-31194573 | None:intergenic | 75.0% |
| ! | TATCAGGGCGGCGGCGGCGG+CGG | + | Chr4:31194569-31194588 | None:intergenic | 80.0% |
| ! | CAGGGCGGCGGCGGCGGCGG+CGG | + | Chr4:31194566-31194585 | None:intergenic | 95.0% |
| ! | GGCGGCGGCGGCGGCGGTGG+TGG | + | Chr4:31194560-31194579 | None:intergenic | 95.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 31194529 | 31195080 | 31194529 | ID=MsG0480019985.01;Name=MsG0480019985.01 |
| Chr4 | mRNA | 31194529 | 31195080 | 31194529 | ID=MsG0480019985.01.T01;Parent=MsG0480019985.01;Name=MsG0480019985.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|183 |
| Chr4 | exon | 31194529 | 31195080 | 31194529 | ID=MsG0480019985.01.T01:exon:10234;Parent=MsG0480019985.01.T01 |
| Chr4 | CDS | 31194529 | 31195080 | 31194529 | ID=MsG0480019985.01.T01:cds;Parent=MsG0480019985.01.T01 |
| Gene Sequence |
| Protein sequence |
