Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022491.01.T01 | XP_003608217.1 | 99.524 | 210 | 1 | 0 | 1 | 210 | 1 | 210 | 5.28E-155 | 437 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048727.01 | MsG0480022491.01 | -0.828551 | 8.443081e-55 | 8.419902e-52 |
| MsG0080048773.01 | MsG0480022491.01 | -0.808833 | 2.549862e-50 | 1.473059e-47 |
| MsG0080048849.01 | MsG0480022491.01 | -0.817444 | 3.287687e-52 | 2.394726e-49 |
| MsG0080048878.01 | MsG0480022491.01 | -0.813339 | 2.690466e-51 | 1.752289e-48 |
| MsG0080049002.01 | MsG0480022491.01 | -0.838020 | 3.684853e-57 | 4.873448e-54 |
| MsG0080049068.01 | MsG0480022491.01 | -0.819209 | 1.309775e-52 | 1.001508e-49 |
| MsG0180000218.01 | MsG0480022491.01 | 0.856707 | 2.682577e-62 | 6.458532e-59 |
| MsG0180000292.01 | MsG0480022491.01 | -0.819930 | 8.964423e-53 | 6.994150e-50 |
| MsG0180001610.01 | MsG0480022491.01 | -0.886002 | 4.996172e-72 | 3.505051e-68 |
| MsG0180001704.01 | MsG0480022491.01 | -0.834636 | 2.673104e-56 | 3.191001e-53 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022491.01.T01 | MTR_4g090890 | 99.524 | 210 | 1 | 0 | 1 | 210 | 1 | 210 | 6.39e-159 | 437 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022491.01.T01 | AT4G05000 | 83.254 | 209 | 35 | 0 | 1 | 209 | 1 | 209 | 1.04e-134 | 376 |
| MsG0480022491.01.T01 | AT4G05000 | 83.254 | 209 | 35 | 0 | 1 | 209 | 1 | 209 | 1.04e-134 | 376 |
| MsG0480022491.01.T01 | AT4G05000 | 83.254 | 209 | 35 | 0 | 1 | 209 | 1 | 209 | 1.04e-134 | 376 |
| MsG0480022491.01.T01 | AT4G21560 | 83.173 | 208 | 35 | 0 | 3 | 210 | 2 | 209 | 2.87e-126 | 355 |
| MsG0480022491.01.T01 | AT4G21560 | 83.173 | 208 | 35 | 0 | 3 | 210 | 2 | 209 | 2.87e-126 | 355 |
| MsG0480022491.01.T01 | AT4G21560 | 83.173 | 208 | 35 | 0 | 3 | 210 | 2 | 209 | 2.87e-126 | 355 |
Find 52 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATGAAGGATTGGATTGGA+AGG | 0.553711 | 4:+73195273 | MsG0480022491.01.T01:CDS |
| AACCCTAGCTTCCACCCTTA+AGG | 0.408396 | 4:+73194953 | MsG0480022491.01.T01:CDS |
| AATCAGTCAAGTGCCAGCAT+TGG | 0.532447 | 4:-73195396 | None:intergenic |
| ACAAGACGGTTAATCGCAGC+CGG | 0.551667 | 4:-73195026 | None:intergenic |
| ACATGACGGAGGTTAAGCTC+TGG | 0.557097 | 4:+73194778 | None:intergenic |
| AGAGATTTGCTGATACCTAT+AGG | 0.426552 | 4:+73194994 | MsG0480022491.01.T01:CDS |
| AGCAGCCGGTGTGTCCACCT+CGG | 0.599861 | 4:+73195091 | MsG0480022491.01.T01:CDS |
| AGCCACAGTGGCAGCCGAGG+TGG | 0.627242 | 4:-73195105 | None:intergenic |
| AGCCACCATGTTAAGTTTCA+AGG | 0.416540 | 4:-73195162 | None:intergenic |
| AGCCGGGCACTCCATCCTAT+AGG | 0.544884 | 4:-73195009 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTGGAAGGTTATCAAAGAT+GGG | + | Chr4:73195287-73195306 | MsG0480022491.01.T01:CDS | 30.0% | |
| GTCTTCTTACAATTCCTTTA+TGG | + | Chr4:73195364-73195383 | MsG0480022491.01.T01:CDS | 30.0% | |
| CAATTATCAAGGCTACTGAA+AGG | + | Chr4:73194850-73194869 | MsG0480022491.01.T01:CDS | 35.0% | |
| CACTCTATCTCATATTCCTT+TGG | - | Chr4:73194909-73194928 | None:intergenic | 35.0% | |
| CGAGGGGAAAATAAAAATGA+AGG | + | Chr4:73195259-73195278 | MsG0480022491.01.T01:CDS | 35.0% | |
| GATTGGAAGGTTATCAAAGA+TGG | + | Chr4:73195286-73195305 | MsG0480022491.01.T01:CDS | 35.0% | |
| GGATTCCTTGAAACTTAACA+TGG | + | Chr4:73195157-73195176 | MsG0480022491.01.T01:CDS | 35.0% | |
| TGATGAATTGACAGAACAAC+AGG | + | Chr4:73195316-73195335 | MsG0480022491.01.T01:CDS | 35.0% | |
| TTCCTTGAAACTTAACATGG+TGG | + | Chr4:73195160-73195179 | MsG0480022491.01.T01:CDS | 35.0% | |
| AGCCACCATGTTAAGTTTCA+AGG | - | Chr4:73195165-73195184 | None:intergenic | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 73194780 | 73195412 | 73194780 | ID=MsG0480022491.01;Name=MsG0480022491.01 |
| Chr4 | mRNA | 73194780 | 73195412 | 73194780 | ID=MsG0480022491.01.T01;Parent=MsG0480022491.01;Name=MsG0480022491.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|210 |
| Chr4 | exon | 73194780 | 73195412 | 73194780 | ID=MsG0480022491.01.T01:exon:10257;Parent=MsG0480022491.01.T01 |
| Chr4 | CDS | 73194780 | 73195412 | 73194780 | ID=MsG0480022491.01.T01:cds;Parent=MsG0480022491.01.T01 |
| Gene Sequence |
>MsG0480022491.01.T01 ATGACGGAGGTTAAGCTCTGGAATGACAAGCGCGAGAGAGAAATGTATGACAACTTTGCTGAGCTTTATGCAATTATCAAGGCTACTGAAAGGCTTGAGAAAGCTTATGTTAGAGATATAATATCACCAAAGGAATATGAGATAGAGTGCCAGAAGCTAATTGCTCATTTCAAAACCCTAGCTTCCACCCTTAAGGACACTGTACCTAGCATTGAGAGATTTGCTGATACCTATAGGATGGAGTGCCCGGCTGCGATTAACCGTCTTGTGGTATCTGGTGTGCCTGCTACAGTGGAGCACCGGGCTACTGCAGCAGCCGGTGTGTCCACCTCGGCTGCCACTGTGGCTGAGTGTGTTCAGAATTTTATTACATCGATGGATTCCTTGAAACTTAACATGGTGGCTGTGGATCAGGTGCATCCTTTGCTCTCAGATCTTTATGGTTCACTCAATAAATTGACGATTTTGCCGCCTGATTTCGAGGGGAAAATAAAAATGAAGGATTGGATTGGAAGGTTATCAAAGATGGGTGCAGCTGATGAATTGACAGAACAACAGGCCAGACAGCTTCACTTTGATCTCGAGTCTTCTTACAATTCCTTTATGGCAGCTCTGCCCAATGCTGGCACTTGA |
| Protein sequence |
>MsG0480022491.01.T01 MTEVKLWNDKREREMYDNFAELYAIIKATERLEKAYVRDIISPKEYEIECQKLIAHFKTLASTLKDTVPSIERFADTYRMECPAAINRLVVSGVPATVEHRATAAAGVSTSAATVAECVQNFITSMDSLKLNMVAVDQVHPLLSDLYGSLNKLTILPPDFEGKIKMKDWIGRLSKMGAADELTEQQARQLHFDLESSYNSFMAALPNAGT* |
