AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022491.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022491.01.T01 MTR_4g090890 99.524 210 1 0 1 210 1 210 6.39e-159 437
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022491.01.T01 AT4G05000 83.254 209 35 0 1 209 1 209 1.04e-134 376
MsG0480022491.01.T01 AT4G05000 83.254 209 35 0 1 209 1 209 1.04e-134 376
MsG0480022491.01.T01 AT4G05000 83.254 209 35 0 1 209 1 209 1.04e-134 376
MsG0480022491.01.T01 AT4G21560 83.173 208 35 0 3 210 2 209 2.87e-126 355
MsG0480022491.01.T01 AT4G21560 83.173 208 35 0 3 210 2 209 2.87e-126 355
MsG0480022491.01.T01 AT4G21560 83.173 208 35 0 3 210 2 209 2.87e-126 355

Find 52 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTGCTCTCAGATCTTTA+TGG 0.115170 4:+73195200 MsG0480022491.01.T01:CDS
GTCTTCTTACAATTCCTTTA+TGG 0.166564 4:+73195364 MsG0480022491.01.T01:CDS
CACTCTATCTCATATTCCTT+TGG 0.322658 4:-73194906 None:intergenic
TGCTAGGTACAGTGTCCTTA+AGG 0.356333 4:-73194968 None:intergenic
TGAAATGAGCAATTAGCTTC+TGG 0.384000 4:-73194929 None:intergenic
ATTAACCGTCTTGTGGTATC+TGG 0.384677 4:+73195035 MsG0480022491.01.T01:CDS
AACCCTAGCTTCCACCCTTA+AGG 0.408396 4:+73194953 MsG0480022491.01.T01:CDS
AGCCACCATGTTAAGTTTCA+AGG 0.416540 4:-73195162 None:intergenic
TGCCTGCTACAGTGGAGCAC+CGG 0.419657 4:+73195060 MsG0480022491.01.T01:CDS
ATTGGGCAGAGCTGCCATAA+AGG 0.420324 4:-73195378 None:intergenic
TACCTATAGGATGGAGTGCC+CGG 0.420784 4:+73195007 MsG0480022491.01.T01:CDS
AGAGATTTGCTGATACCTAT+AGG 0.426552 4:+73194994 MsG0480022491.01.T01:CDS
TGATGAATTGACAGAACAAC+AGG 0.433481 4:+73195316 MsG0480022491.01.T01:CDS
GATTGGAAGGTTATCAAAGA+TGG 0.440404 4:+73195286 MsG0480022491.01.T01:CDS
TAACATGGTGGCTGTGGATC+AGG 0.449737 4:+73195172 MsG0480022491.01.T01:CDS
TGTCCTTAAGGGTGGAAGCT+AGG 0.450461 4:-73194956 None:intergenic
CACCGGCTGCTGCAGTAGCC+CGG 0.452909 4:-73195079 None:intergenic
ATGGCAGCTCTGCCCAATGC+TGG 0.461234 4:+73195383 MsG0480022491.01.T01:CDS
CACCGGGCTACTGCAGCAGC+CGG 0.465917 4:+73195077 MsG0480022491.01.T01:CDS
GAGATCAAAGTGAAGCTGTC+TGG 0.466167 4:-73195339 None:intergenic
ATCTGGTGTGCCTGCTACAG+TGG 0.466901 4:+73195052 MsG0480022491.01.T01:CDS
GCCCGGTGCTCCACTGTAGC+AGG 0.469416 4:-73195062 None:intergenic
GTCCTTAAGGGTGGAAGCTA+GGG 0.470135 4:-73194955 None:intergenic
GCTAGGTACAGTGTCCTTAA+GGG 0.471651 4:-73194967 None:intergenic
ATCAGTCAAGTGCCAGCATT+GGG 0.475748 4:-73195395 None:intergenic
TGAGCTTTATGCAATTATCA+AGG 0.477470 4:+73194839 MsG0480022491.01.T01:CDS
GCCTGCTACAGTGGAGCACC+GGG 0.503675 4:+73195061 MsG0480022491.01.T01:CDS
GAAACTTAACATGGTGGCTG+TGG 0.505970 4:+73195166 MsG0480022491.01.T01:CDS
AATCAGTCAAGTGCCAGCAT+TGG 0.532447 4:-73195396 None:intergenic
AGCCGGGCACTCCATCCTAT+AGG 0.544884 4:-73195009 None:intergenic
ACAAGACGGTTAATCGCAGC+CGG 0.551667 4:-73195026 None:intergenic
AAATGAAGGATTGGATTGGA+AGG 0.553711 4:+73195273 MsG0480022491.01.T01:CDS
ACATGACGGAGGTTAAGCTC+TGG 0.557097 4:+73194778 None:intergenic
TCAGCAAATCTCTCAATGCT+AGG 0.562437 4:-73194984 None:intergenic
ATTGGAAGGTTATCAAAGAT+GGG 0.567981 4:+73195287 MsG0480022491.01.T01:CDS
ATTTGCTGATACCTATAGGA+TGG 0.575681 4:+73194998 MsG0480022491.01.T01:CDS
CTCAGCCACAGTGGCAGCCG+AGG 0.584044 4:-73195108 None:intergenic
GGCAGCCGAGGTGGACACAC+CGG 0.587917 4:-73195096 None:intergenic
GTCCACCTCGGCTGCCACTG+TGG 0.597134 4:+73195103 MsG0480022491.01.T01:CDS
AGCAGCCGGTGTGTCCACCT+CGG 0.599861 4:+73195091 MsG0480022491.01.T01:CDS
CCATAAAGATCTGAGAGCAA+AGG 0.601434 4:-73195200 None:intergenic
TAGAGATATAATATCACCAA+AGG 0.609606 4:+73194890 MsG0480022491.01.T01:CDS
AGCCACAGTGGCAGCCGAGG+TGG 0.627242 4:-73195105 None:intergenic
AGGTACAGTGTCCTTAAGGG+TGG 0.630756 4:-73194964 None:intergenic
GGATTCCTTGAAACTTAACA+TGG 0.637083 4:+73195157 MsG0480022491.01.T01:CDS
CAAGACGGTTAATCGCAGCC+GGG 0.658456 4:-73195025 None:intergenic
CAATTATCAAGGCTACTGAA+AGG 0.659301 4:+73194850 MsG0480022491.01.T01:CDS
GGCTGCGATTAACCGTCTTG+TGG 0.665895 4:+73195028 MsG0480022491.01.T01:CDS
TAGAATAAATAACATGACGG+AGG 0.669493 4:+73194767 None:intergenic
CTGAACACACTCAGCCACAG+TGG 0.699627 4:-73195117 None:intergenic
TTCCTTGAAACTTAACATGG+TGG 0.700241 4:+73195160 MsG0480022491.01.T01:CDS
GCACACCAGATACCACAAGA+CGG 0.739666 4:-73195040 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATAAAAATGAAGGATTGGAT+TGG + Chr4:73195269-73195288 MsG0480022491.01.T01:CDS 25.0%
! GGAAAATAAAAATGAAGGAT+TGG + Chr4:73195264-73195283 MsG0480022491.01.T01:CDS 25.0%
! TAGAGATATAATATCACCAA+AGG + Chr4:73194890-73194909 MsG0480022491.01.T01:CDS 25.0%
!! TCAGAATTTTATTACATCGA+TGG + Chr4:73195136-73195155 MsG0480022491.01.T01:CDS 25.0%
ATTGGAAGGTTATCAAAGAT+GGG + Chr4:73195287-73195306 MsG0480022491.01.T01:CDS 30.0%
GTCTTCTTACAATTCCTTTA+TGG + Chr4:73195364-73195383 MsG0480022491.01.T01:CDS 30.0%
! TGAGCTTTATGCAATTATCA+AGG + Chr4:73194839-73194858 MsG0480022491.01.T01:CDS 30.0%
CAATTATCAAGGCTACTGAA+AGG + Chr4:73194850-73194869 MsG0480022491.01.T01:CDS 35.0%
CACTCTATCTCATATTCCTT+TGG - Chr4:73194909-73194928 None:intergenic 35.0%
CGAGGGGAAAATAAAAATGA+AGG + Chr4:73195259-73195278 MsG0480022491.01.T01:CDS 35.0%
GATTGGAAGGTTATCAAAGA+TGG + Chr4:73195286-73195305 MsG0480022491.01.T01:CDS 35.0%
GGATTCCTTGAAACTTAACA+TGG + Chr4:73195157-73195176 MsG0480022491.01.T01:CDS 35.0%
TGATGAATTGACAGAACAAC+AGG + Chr4:73195316-73195335 MsG0480022491.01.T01:CDS 35.0%
TTCCTTGAAACTTAACATGG+TGG + Chr4:73195160-73195179 MsG0480022491.01.T01:CDS 35.0%
! AAATGAAGGATTGGATTGGA+AGG + Chr4:73195273-73195292 MsG0480022491.01.T01:CDS 35.0%
! ATTTGCTGATACCTATAGGA+TGG + Chr4:73194998-73195017 MsG0480022491.01.T01:CDS 35.0%
! TGAAATGAGCAATTAGCTTC+TGG - Chr4:73194932-73194951 None:intergenic 35.0%
!! AGAGATTTGCTGATACCTAT+AGG + Chr4:73194994-73195013 MsG0480022491.01.T01:CDS 35.0%
!! TTTATTTTCCCCTCGAAATC+AGG - Chr4:73195254-73195273 None:intergenic 35.0%
AGCCACCATGTTAAGTTTCA+AGG - Chr4:73195165-73195184 None:intergenic 40.0%
ATTAACCGTCTTGTGGTATC+TGG + Chr4:73195035-73195054 MsG0480022491.01.T01:CDS 40.0%
CCATAAAGATCTGAGAGCAA+AGG - Chr4:73195203-73195222 None:intergenic 40.0%
CCTTTGCTCTCAGATCTTTA+TGG + Chr4:73195200-73195219 MsG0480022491.01.T01:CDS 40.0%
TCAGCAAATCTCTCAATGCT+AGG - Chr4:73194987-73195006 None:intergenic 40.0%
GAAACTTAACATGGTGGCTG+TGG + Chr4:73195166-73195185 MsG0480022491.01.T01:CDS 45.0%
GAGATCAAAGTGAAGCTGTC+TGG - Chr4:73195342-73195361 None:intergenic 45.0%
GCTAGGTACAGTGTCCTTAA+GGG - Chr4:73194970-73194989 None:intergenic 45.0%
TGCTAGGTACAGTGTCCTTA+AGG - Chr4:73194971-73194990 None:intergenic 45.0%
! ATTTTCCCCTCGAAATCAGG+CGG - Chr4:73195251-73195270 None:intergenic 45.0%
! ATTTTGCCGCCTGATTTCGA+GGG + Chr4:73195242-73195261 MsG0480022491.01.T01:CDS 45.0%
AACCCTAGCTTCCACCCTTA+AGG + Chr4:73194953-73194972 MsG0480022491.01.T01:CDS 50.0%
ACAAGACGGTTAATCGCAGC+CGG - Chr4:73195029-73195048 None:intergenic 50.0%
AGGTACAGTGTCCTTAAGGG+TGG - Chr4:73194967-73194986 None:intergenic 50.0%
ATTGGGCAGAGCTGCCATAA+AGG - Chr4:73195381-73195400 None:intergenic 50.0%
GCACACCAGATACCACAAGA+CGG - Chr4:73195043-73195062 None:intergenic 50.0%
GTCCTTAAGGGTGGAAGCTA+GGG - Chr4:73194958-73194977 None:intergenic 50.0%
TAACATGGTGGCTGTGGATC+AGG + Chr4:73195172-73195191 MsG0480022491.01.T01:CDS 50.0%
TGTCCTTAAGGGTGGAAGCT+AGG - Chr4:73194959-73194978 None:intergenic 50.0%
! GATTTTGCCGCCTGATTTCG+AGG + Chr4:73195241-73195260 MsG0480022491.01.T01:CDS 50.0%
! TACCTATAGGATGGAGTGCC+CGG + Chr4:73195007-73195026 MsG0480022491.01.T01:CDS 50.0%
! TTTTGCCGCCTGATTTCGAG+GGG + Chr4:73195243-73195262 MsG0480022491.01.T01:CDS 50.0%
ATCTGGTGTGCCTGCTACAG+TGG + Chr4:73195052-73195071 MsG0480022491.01.T01:CDS 55.0%
CAAGACGGTTAATCGCAGCC+GGG - Chr4:73195028-73195047 None:intergenic 55.0%
CTGAACACACTCAGCCACAG+TGG - Chr4:73195120-73195139 None:intergenic 55.0%
GGCTGCGATTAACCGTCTTG+TGG + Chr4:73195028-73195047 MsG0480022491.01.T01:CDS 55.0%
AGCCGGGCACTCCATCCTAT+AGG - Chr4:73195012-73195031 None:intergenic 60.0%
ATGGCAGCTCTGCCCAATGC+TGG + Chr4:73195383-73195402 MsG0480022491.01.T01:CDS 60.0%
TGCCTGCTACAGTGGAGCAC+CGG + Chr4:73195060-73195079 MsG0480022491.01.T01:CDS 60.0%
AGCAGCCGGTGTGTCCACCT+CGG + Chr4:73195091-73195110 MsG0480022491.01.T01:CDS 65.0%
! GCCTGCTACAGTGGAGCACC+GGG + Chr4:73195061-73195080 MsG0480022491.01.T01:CDS 65.0%
AGCCACAGTGGCAGCCGAGG+TGG - Chr4:73195108-73195127 None:intergenic 70.0%
CACCGGGCTACTGCAGCAGC+CGG + Chr4:73195077-73195096 MsG0480022491.01.T01:CDS 70.0%
CTCAGCCACAGTGGCAGCCG+AGG - Chr4:73195111-73195130 None:intergenic 70.0%
GCCCGGTGCTCCACTGTAGC+AGG - Chr4:73195065-73195084 None:intergenic 70.0%
GGCAGCCGAGGTGGACACAC+CGG - Chr4:73195099-73195118 None:intergenic 70.0%
GTCCACCTCGGCTGCCACTG+TGG + Chr4:73195103-73195122 MsG0480022491.01.T01:CDS 70.0%
! CACCGGCTGCTGCAGTAGCC+CGG - Chr4:73195082-73195101 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 73194780 73195412 73194780 ID=MsG0480022491.01;Name=MsG0480022491.01
Chr4 mRNA 73194780 73195412 73194780 ID=MsG0480022491.01.T01;Parent=MsG0480022491.01;Name=MsG0480022491.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|210
Chr4 exon 73194780 73195412 73194780 ID=MsG0480022491.01.T01:exon:10257;Parent=MsG0480022491.01.T01
Chr4 CDS 73194780 73195412 73194780 ID=MsG0480022491.01.T01:cds;Parent=MsG0480022491.01.T01
Gene Sequence

>MsG0480022491.01.T01

ATGACGGAGGTTAAGCTCTGGAATGACAAGCGCGAGAGAGAAATGTATGACAACTTTGCTGAGCTTTATGCAATTATCAAGGCTACTGAAAGGCTTGAGAAAGCTTATGTTAGAGATATAATATCACCAAAGGAATATGAGATAGAGTGCCAGAAGCTAATTGCTCATTTCAAAACCCTAGCTTCCACCCTTAAGGACACTGTACCTAGCATTGAGAGATTTGCTGATACCTATAGGATGGAGTGCCCGGCTGCGATTAACCGTCTTGTGGTATCTGGTGTGCCTGCTACAGTGGAGCACCGGGCTACTGCAGCAGCCGGTGTGTCCACCTCGGCTGCCACTGTGGCTGAGTGTGTTCAGAATTTTATTACATCGATGGATTCCTTGAAACTTAACATGGTGGCTGTGGATCAGGTGCATCCTTTGCTCTCAGATCTTTATGGTTCACTCAATAAATTGACGATTTTGCCGCCTGATTTCGAGGGGAAAATAAAAATGAAGGATTGGATTGGAAGGTTATCAAAGATGGGTGCAGCTGATGAATTGACAGAACAACAGGCCAGACAGCTTCACTTTGATCTCGAGTCTTCTTACAATTCCTTTATGGCAGCTCTGCCCAATGCTGGCACTTGA

Protein sequence

>MsG0480022491.01.T01

MTEVKLWNDKREREMYDNFAELYAIIKATERLEKAYVRDIISPKEYEIECQKLIAHFKTLASTLKDTVPSIERFADTYRMECPAAINRLVVSGVPATVEHRATAAAGVSTSAATVAECVQNFITSMDSLKLNMVAVDQVHPLLSDLYGSLNKLTILPPDFEGKIKMKDWIGRLSKMGAADELTEQQARQLHFDLESSYNSFMAALPNAGT*