AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020128.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020128.01.T01 MTR_6g016335 94.672 244 13 0 51 294 192 435 3.19e-171 483
MsG0480020128.01.T01 MTR_1g102550 76.894 264 61 0 31 294 150 413 9.77e-149 426
MsG0480020128.01.T01 MTR_6g016320 88.710 62 7 0 184 245 11 72 1.29e-34 121
MsG0480020128.01.T01 MTR_6g016325 92.157 51 4 0 244 294 30 80 4.03e-26 100
MsG0480020128.01.T01 MTR_1g083640 86.957 46 6 0 1 46 1 46 8.29e-17 79.3
MsG0480020128.01.T01 MTR_5g071590 73.913 46 9 1 1 46 1 43 9.68e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020128.01.T01 AT1G19920 87.295 244 31 0 51 294 182 425 1.46e-160 456
MsG0480020128.01.T01 AT3G22890 81.707 246 45 0 49 294 166 411 2.05e-149 427
MsG0480020128.01.T01 AT4G14680 75.735 272 59 1 23 294 149 413 1.97e-148 425
MsG0480020128.01.T01 AT4G14680 75.735 272 59 1 23 294 149 413 1.01e-147 424
MsG0480020128.01.T01 AT5G43780 81.557 244 45 0 51 294 172 415 1.35e-147 423

Find 74 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGCTGGAAATTGGCTTAT+TGG 0.229141 4:-34665370 MsG0480020128.01.T01:CDS
TGTTCATAGGTCCTAGAATA+TGG 0.265617 4:-34664544 MsG0480020128.01.T01:intron
AGAGGATGAAGCAATAAAAT+TGG 0.269794 4:+34665142 None:intergenic
TCATTGCTCAAGCTGGAAAT+TGG 0.277899 4:-34665378 MsG0480020128.01.T01:CDS
TTGGCTTATTGGAGGAGATT+TGG 0.324311 4:-34665359 MsG0480020128.01.T01:CDS
GATCAAGAACTCCATATTCT+AGG 0.334671 4:+34664533 None:intergenic
GAATTCTTCACAAAGTTGTT+TGG 0.340648 4:+34665282 None:intergenic
AATTCTTCACAAAGTTGTTT+GGG 0.377029 4:+34665283 None:intergenic
AGACCATCATTGTATTTGAT+AGG 0.381497 4:+34665325 None:intergenic
TGTTACTCCCCATTGAAATC+AGG 0.389401 4:-34665992 MsG0480020128.01.T01:CDS
TGCCTTTCAGTTAAGAAATC+CGG 0.399099 4:-34665233 MsG0480020128.01.T01:CDS
TCCTCAACGTAAGGCAATCC+TGG 0.418206 4:+34665403 None:intergenic
ACGATGTTCCGTTAGATGTC+AGG 0.428955 4:-34665099 MsG0480020128.01.T01:CDS
TGGTCGCGATCCCACTGGTA+TGG 0.437184 4:-34664398 MsG0480020128.01.T01:CDS
ATAGAAACAATGGTTGTCTC+TGG 0.438582 4:+34664511 None:intergenic
TTATTGCTTCATCCTCTTGG+AGG 0.441232 4:-34665136 MsG0480020128.01.T01:CDS
CCATTGCCTATGTATTATGC+AGG 0.449264 4:-34664484 MsG0480020128.01.T01:CDS
GCATGCCATTGTACTTCAGT+TGG 0.450436 4:+34664460 None:intergenic
GGGTCATCCAACTGAGAAAC+GGG 0.451624 4:-34664377 MsG0480020128.01.T01:CDS
TCCTGGAGCAGTTGTTCCCC+AGG 0.464917 4:+34665420 None:intergenic
AACTTTGTGAAGAATTCGAT+TGG 0.472770 4:-34665276 MsG0480020128.01.T01:CDS
TGTCCCACCCTGATTTCAAT+GGG 0.474532 4:+34665984 None:intergenic
GGTGCCAACTTCTATATTGT+TGG 0.475062 4:-34664418 MsG0480020128.01.T01:CDS
GTGTCCCACCCTGATTTCAA+TGG 0.480555 4:+34665983 None:intergenic
GACTTGTATGACCCTGATCA+TGG 0.490265 4:-34664355 MsG0480020128.01.T01:CDS
TACAAGTCCCGTTTCTCAGT+TGG 0.492506 4:+34664370 None:intergenic
TGGGTCATCCAACTGAGAAA+CGG 0.499633 4:-34664378 MsG0480020128.01.T01:CDS
AAGTCATGACCATTATGAAC+CGG 0.501877 4:+34665214 None:intergenic
ACCTGGGGAACAACTGCTCC+AGG 0.502578 4:-34665421 MsG0480020128.01.T01:CDS
GCAATACTACTTACCCTGAA+TGG 0.502969 4:+34664283 None:intergenic
GGTCGCGATCCCACTGGTAT+GGG 0.507074 4:-34664397 MsG0480020128.01.T01:CDS
AAACCTATCAAATACAATGA+TGG 0.508307 4:-34665328 MsG0480020128.01.T01:CDS
TTAAGAAATCCGGTTCATAA+TGG 0.508889 4:-34665223 MsG0480020128.01.T01:CDS
AAGAAAGAATAGCTAGAACC+TGG 0.509563 4:-34665438 MsG0480020128.01.T01:CDS
TGTCCACTCTCACCATGATT+CGG 0.518699 4:+34666061 None:intergenic
GCTGGAAATTGGCTTATTGG+AGG 0.523653 4:-34665367 MsG0480020128.01.T01:CDS
ATGATGGTCTTGATAACTAC+AGG 0.527746 4:-34665312 MsG0480020128.01.T01:CDS
AAGGTGCGTAACATGGCTCC+TGG 0.533033 4:-34664328 MsG0480020128.01.T01:CDS
AGAAAGAATAGCTAGAACCT+GGG 0.539463 4:-34665437 MsG0480020128.01.T01:CDS
GAAACTTCATCACCGAATCA+TGG 0.540312 4:-34666073 MsG0480020128.01.T01:CDS
ATGTTAAGCTTCTCGAGACC+AGG 0.540824 4:+34664310 None:intergenic
AACCGGATTTCTTAACTGAA+AGG 0.545427 4:+34665231 None:intergenic
TCCAGGATTGCCTTACGTTG+AGG 0.546081 4:-34665404 MsG0480020128.01.T01:CDS
GTTACTCCCCATTGAAATCA+GGG 0.556533 4:-34665991 MsG0480020128.01.T01:intron
GCGACCAACAATATAGAAGT+TGG 0.559340 4:+34664414 None:intergenic
GAGGAGGTCATTGCTCAAGC+TGG 0.561029 4:-34665385 MsG0480020128.01.T01:CDS
GCAAAGTCACGGATAAATGT+AGG 0.561922 4:-34664439 MsG0480020128.01.T01:CDS
ATTGTTGGTCGCGATCCCAC+TGG 0.563013 4:-34664403 MsG0480020128.01.T01:CDS
TGCTCCATCCTGACATCTAA+CGG 0.570183 4:+34665091 None:intergenic
CATTGCCTATGTATTATGCA+GGG 0.574572 4:-34664483 MsG0480020128.01.T01:CDS
AGAAAGCTTTAGCACGGCTA+GGG 0.581287 4:+34663808 None:intergenic
TCACCGAATCATGGTGAGAG+TGG 0.582611 4:-34666064 MsG0480020128.01.T01:CDS
TAGAAAGCTTTAGCACGGCT+AGG 0.584667 4:+34663807 None:intergenic
GTTGGCCCTGCATAATACAT+AGG 0.586454 4:+34664478 None:intergenic
TGACACTAAGGTAAACAAGA+TGG 0.598361 4:-34663841 MsG0480020128.01.T01:CDS
CAATGGAAATATAGAAACAA+TGG 0.598407 4:+34664501 None:intergenic
TCGTCAACGTCTATTAGACA+TGG 0.599665 4:-34665176 MsG0480020128.01.T01:CDS
TACAATGGCATGCAAAGTCA+CGG 0.599759 4:-34664450 MsG0480020128.01.T01:CDS
GGTGGCAGCTTATGACACTA+AGG 0.610562 4:-34663853 MsG0480020128.01.T01:CDS
ATATCAACTTCATCTGAGAG+TGG 0.614980 4:-34666109 MsG0480020128.01.T01:CDS
CCTGCATAATACATAGGCAA+TGG 0.618039 4:+34664484 None:intergenic
CAGGATGGAGCAACATAGCA+AGG 0.622612 4:-34665080 MsG0480020128.01.T01:intron
GCAATGACCTCCTCAACGTA+AGG 0.623338 4:+34665394 None:intergenic
AGGATTGCCTTACGTTGAGG+AGG 0.626411 4:-34665401 MsG0480020128.01.T01:CDS
GAAAGAATAGCTAGAACCTG+GGG 0.638025 4:-34665436 MsG0480020128.01.T01:CDS
ACGTGTAGAAAGCTTTAGCA+CGG 0.651162 4:+34663802 None:intergenic
GTTGGATGACCCATACCAGT+GGG 0.656888 4:+34664388 None:intergenic
TCCTCTTGGAGGTTTCACAA+AGG 0.657993 4:-34665125 MsG0480020128.01.T01:CDS
CAGGGCCAACTGAAGTACAA+TGG 0.658851 4:-34664465 MsG0480020128.01.T01:CDS
CGTCAACGTCTATTAGACAT+GGG 0.666925 4:-34665175 MsG0480020128.01.T01:CDS
TGTTCCGTTAGATGTCAGGA+TGG 0.672939 4:-34665095 MsG0480020128.01.T01:CDS
AGTTGGATGACCCATACCAG+TGG 0.682696 4:+34664387 None:intergenic
ACCTTTGTGAAACCTCCAAG+AGG 0.706301 4:+34665124 None:intergenic
GTCCCACCCTGATTTCAATG+GGG 0.706569 4:+34665985 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAAACATTGATTATTAT+AGG + Chr4:34665091-34665110 None:intergenic 10.0%
!! ATAAAAGAATTACAAATGAA+CGG + Chr4:34665158-34665177 None:intergenic 15.0%
!!! TGAAATTTTGTACAAAAAAA+TGG + Chr4:34665860-34665879 None:intergenic 15.0%
!! AATGTTTAAAAAATTGACCT+TGG - Chr4:34665100-34665119 MsG0480020128.01.T01:CDS 20.0%
!! ATGTTAAAAAATGAACAAGA+TGG + Chr4:34664308-34664327 None:intergenic 20.0%
!! GATTATAATAGCATGTAATT+TGG + Chr4:34665767-34665786 None:intergenic 20.0%
!! TTACATGCTATTATAATCTT+CGG - Chr4:34665769-34665788 MsG0480020128.01.T01:intron 20.0%
!!! AAATTCTTTTTCTTGTTCAT+AGG - Chr4:34665346-34665365 MsG0480020128.01.T01:CDS 20.0%
!!! CGATTAATTTTTTAAAGCAT+TGG - Chr4:34665306-34665325 MsG0480020128.01.T01:CDS 20.0%
! AAACCTATCAAATACAATGA+TGG - Chr4:34664575-34664594 MsG0480020128.01.T01:intron 25.0%
! AAAGAAATTGAACTATGACT+CGG + Chr4:34664197-34664216 None:intergenic 25.0%
! AACAAACAAGAAAGATCTAT+AGG + Chr4:34665283-34665302 None:intergenic 25.0%
! AATTCTTCACAAAGTTGTTT+GGG + Chr4:34664623-34664642 None:intergenic 25.0%
! ACACCATATATCTTATTGTT+GGG - Chr4:34665886-34665905 MsG0480020128.01.T01:intron 25.0%
! ACTTTGTATACTAAAGAACA+AGG + Chr4:34665932-34665951 None:intergenic 25.0%
! CAATGGAAATATAGAAACAA+TGG + Chr4:34665405-34665424 None:intergenic 25.0%
! CATATAAGATTGAAAATCGT+AGG + Chr4:34664082-34664101 None:intergenic 25.0%
! GCTATATGAGAAAAAAATGA+TGG + Chr4:34664855-34664874 None:intergenic 25.0%
! GTAATATAAAATGGCAAACA+AGG + Chr4:34666029-34666048 None:intergenic 25.0%
! TAATAGCATGTAATTTGGTT+CGG + Chr4:34665762-34665781 None:intergenic 25.0%
! TACACCATATATCTTATTGT+TGG - Chr4:34665885-34665904 MsG0480020128.01.T01:intron 25.0%
! TTCTTTAAACTACTCAAACT+AGG - Chr4:34664211-34664230 MsG0480020128.01.T01:intron 25.0%
!! CAACTTATCAGCAAAATTTT+AGG + Chr4:34665984-34666003 None:intergenic 25.0%
!! CTAAAATTTTGCTGATAAGT+TGG - Chr4:34665982-34666001 MsG0480020128.01.T01:intron 25.0%
!! GCTGAATTTTTGAATTACAA+TGG - Chr4:34664328-34664347 MsG0480020128.01.T01:CDS 25.0%
!! TTTTTGAATTACAATGGCTA+TGG - Chr4:34664334-34664353 MsG0480020128.01.T01:CDS 25.0%
!!! TGTTTGCCATTTTATATTAC+AGG - Chr4:34666029-34666048 MsG0480020128.01.T01:CDS 25.0%
!!! TTCAGCTTTTATTGATTTTG+AGG + Chr4:34665811-34665830 None:intergenic 25.0%
AACTTTGTGAAGAATTCGAT+TGG - Chr4:34664627-34664646 MsG0480020128.01.T01:intron 30.0%
AATAATCAGTAGACAGTTCT+TGG + Chr4:34665210-34665229 None:intergenic 30.0%
AGACCATCATTGTATTTGAT+AGG + Chr4:34664581-34664600 None:intergenic 30.0%
AGAGGATGAAGCAATAAAAT+TGG + Chr4:34664764-34664783 None:intergenic 30.0%
ATCCCCAACAATAAGATATA+TGG + Chr4:34665892-34665911 None:intergenic 30.0%
ATCTCAGTTCATGGTTTAAT+TGG - Chr4:34664277-34664296 MsG0480020128.01.T01:intron 30.0%
ATTTAAGTAAACTCCTGTCA+TGG - Chr4:34664359-34664378 MsG0480020128.01.T01:CDS 30.0%
CACCATATATCTTATTGTTG+GGG - Chr4:34665887-34665906 MsG0480020128.01.T01:intron 30.0%
CTGCAAAGAAGTGAAATTAA+TGG + Chr4:34664419-34664438 None:intergenic 30.0%
GAAGTGAAATTAATGGTCAT+TGG + Chr4:34664412-34664431 None:intergenic 30.0%
GAATTCTTCACAAAGTTGTT+TGG + Chr4:34664624-34664643 None:intergenic 30.0%
TAAAAAATTGACCTTGGAAG+TGG - Chr4:34665106-34665125 MsG0480020128.01.T01:CDS 30.0%
TATTCAACTCAGTGTATGTT+TGG + Chr4:34663973-34663992 None:intergenic 30.0%
TTAAGAAATCCGGTTCATAA+TGG - Chr4:34664680-34664699 MsG0480020128.01.T01:intron 30.0%
TTATTCGAGAAGTTAGATTG+CGG - Chr4:34665225-34665244 MsG0480020128.01.T01:CDS 30.0%
! ATTTTATTGCTTCATCCTCT+TGG - Chr4:34664764-34664783 MsG0480020128.01.T01:intron 30.0%
!! TTGACTTGGATCTGTTTATA+CGG + Chr4:34665701-34665720 None:intergenic 30.0%
!!! CAATGTTTTAGAAATCGAAC+CGG - Chr4:34664151-34664170 MsG0480020128.01.T01:intron 30.0%
!!! TTGCCATTTTATATTACAGG+TGG - Chr4:34666032-34666051 MsG0480020128.01.T01:CDS 30.0%
AAAAATGGACAGCATTGTGA+AGG + Chr4:34665845-34665864 None:intergenic 35.0%
AAAATGGACAGCATTGTGAA+GGG + Chr4:34665844-34665863 None:intergenic 35.0%
AACCGGATTTCTTAACTGAA+AGG + Chr4:34664675-34664694 None:intergenic 35.0%
AACCTCCCAAATAACTTAAC+CGG - Chr4:34664249-34664268 MsG0480020128.01.T01:intron 35.0%
AAGAAAGAATAGCTAGAACC+TGG - Chr4:34664465-34664484 MsG0480020128.01.T01:CDS 35.0%
AAGTCATGACCATTATGAAC+CGG + Chr4:34664692-34664711 None:intergenic 35.0%
AGAAAGAATAGCTAGAACCT+GGG - Chr4:34664466-34664485 MsG0480020128.01.T01:CDS 35.0%
ATAGAAACAATGGTTGTCTC+TGG + Chr4:34665395-34665414 None:intergenic 35.0%
ATATCAACTTCATCTGAGAG+TGG - Chr4:34663794-34663813 MsG0480020128.01.T01:CDS 35.0%
CAAGAAAGATCTATAGGTCA+TGG + Chr4:34665277-34665296 None:intergenic 35.0%
CATTGCCTATGTATTATGCA+GGG - Chr4:34665420-34665439 MsG0480020128.01.T01:CDS 35.0%
CGATAAGTTAGGCAAAATCA+TGG + Chr4:34663945-34663964 None:intergenic 35.0%
CTGCCACCTGTAATATAAAA+TGG + Chr4:34666038-34666057 None:intergenic 35.0%
GATCAAGAACTCCATATTCT+AGG + Chr4:34665373-34665392 None:intergenic 35.0%
GTTTGGATTGACGATAAGTT+AGG + Chr4:34663956-34663975 None:intergenic 35.0%
TGACACTAAGGTAAACAAGA+TGG - Chr4:34666062-34666081 MsG0480020128.01.T01:CDS 35.0%
TGCCTTTCAGTTAAGAAATC+CGG - Chr4:34664670-34664689 MsG0480020128.01.T01:intron 35.0%
TGGTTAAACTGCTTTATACC+AGG + Chr4:34665257-34665276 None:intergenic 35.0%
TGTCTCCGGTTAAGTTATTT+GGG + Chr4:34664257-34664276 None:intergenic 35.0%
TGTTCATAGGTCCTAGAATA+TGG - Chr4:34665359-34665378 MsG0480020128.01.T01:CDS 35.0%
TTATTGTTGGGGATGACAAA+TGG - Chr4:34665898-34665917 MsG0480020128.01.T01:intron 35.0%
TTATTTGGGAGGTTCAACTA+CGG + Chr4:34664243-34664262 None:intergenic 35.0%
TTCAACATGTCGAAACTTAC+TGG + Chr4:34665181-34665200 None:intergenic 35.0%
TTGTCTCCGGTTAAGTTATT+TGG + Chr4:34664258-34664277 None:intergenic 35.0%
! AGCTTAACATTTTGCCATTC+AGG - Chr4:34665606-34665625 MsG0480020128.01.T01:intron 35.0%
! ATGATGGTCTTGATAACTAC+AGG - Chr4:34664591-34664610 MsG0480020128.01.T01:intron 35.0%
! GCTTAACATTTTGCCATTCA+GGG - Chr4:34665607-34665626 MsG0480020128.01.T01:intron 35.0%
! TTGTCAAATAGCAGCTTTAG+CGG - Chr4:34664973-34664992 MsG0480020128.01.T01:intron 35.0%
AAACTCAAAGACACCATGAC+AGG + Chr4:34664375-34664394 None:intergenic 40.0%
ATACCTGTGCTATAGAGTAG+CGG - Chr4:34664900-34664919 MsG0480020128.01.T01:intron 40.0%
CCATTGCCTATGTATTATGC+AGG - Chr4:34665419-34665438 MsG0480020128.01.T01:CDS 40.0%
CCTGCATAATACATAGGCAA+TGG + Chr4:34665422-34665441 None:intergenic 40.0%
CGTCAACGTCTATTAGACAT+GGG - Chr4:34664728-34664747 MsG0480020128.01.T01:intron 40.0%
GAAACTTCATCACCGAATCA+TGG - Chr4:34663830-34663849 MsG0480020128.01.T01:CDS 40.0%
GAAAGAATAGCTAGAACCTG+GGG - Chr4:34664467-34664486 MsG0480020128.01.T01:CDS 40.0%
GCAAAGTCACGGATAAATGT+AGG - Chr4:34665464-34665483 MsG0480020128.01.T01:CDS 40.0%
GCAATACTACTTACCCTGAA+TGG + Chr4:34665623-34665642 None:intergenic 40.0%
GCGACCAACAATATAGAAGT+TGG + Chr4:34665492-34665511 None:intergenic 40.0%
GGTGCCAACTTCTATATTGT+TGG - Chr4:34665485-34665504 MsG0480020128.01.T01:intron 40.0%
GTTACTCCCCATTGAAATCA+GGG - Chr4:34663912-34663931 MsG0480020128.01.T01:intron 40.0%
TACAATGGCATGCAAAGTCA+CGG - Chr4:34665453-34665472 MsG0480020128.01.T01:CDS 40.0%
TCATTGCTCAAGCTGGAAAT+TGG - Chr4:34664525-34664544 MsG0480020128.01.T01:CDS 40.0%
TCGTCAACGTCTATTAGACA+TGG - Chr4:34664727-34664746 MsG0480020128.01.T01:intron 40.0%
TGACCCTGATCATGGAAAAA+AGG - Chr4:34665556-34665575 MsG0480020128.01.T01:intron 40.0%
TGTTACTCCCCATTGAAATC+AGG - Chr4:34663911-34663930 MsG0480020128.01.T01:intron 40.0%
TTAACCAGTGTTGTCAATCG+TGG + Chr4:34665053-34665072 None:intergenic 40.0%
TTATTGCTTCATCCTCTTGG+AGG - Chr4:34664767-34664786 MsG0480020128.01.T01:intron 40.0%
TTGGCTTATTGGAGGAGATT+TGG - Chr4:34664544-34664563 MsG0480020128.01.T01:intron 40.0%
! ACGTGTAGAAAGCTTTAGCA+CGG + Chr4:34666104-34666123 None:intergenic 40.0%
! CAAGCTGGAAATTGGCTTAT+TGG - Chr4:34664533-34664552 MsG0480020128.01.T01:CDS 40.0%
! GAAATTGAACTATGACTCGG+AGG + Chr4:34664194-34664213 None:intergenic 40.0%
! TGGAAAAAAGGTGCGTAACA+TGG - Chr4:34665568-34665587 MsG0480020128.01.T01:intron 40.0%
!! CGTTGAAGTGTTGTTTGACT+TGG + Chr4:34665715-34665734 None:intergenic 40.0%
!!! GCACCTTTTTTCCATGATCA+GGG + Chr4:34665562-34665581 None:intergenic 40.0%
AAACCGCTACTCTATAGCAC+AGG + Chr4:34664906-34664925 None:intergenic 45.0%
ACCTTTGTGAAACCTCCAAG+AGG + Chr4:34664782-34664801 None:intergenic 45.0%
ACGATGTTCCGTTAGATGTC+AGG - Chr4:34664804-34664823 MsG0480020128.01.T01:intron 45.0%
ATGTTAAGCTTCTCGAGACC+AGG + Chr4:34665596-34665615 None:intergenic 45.0%
CAATCCACGATTGACAACAC+TGG - Chr4:34665046-34665065 MsG0480020128.01.T01:intron 45.0%
CCATGAACTGAGATTGTCTC+CGG + Chr4:34664271-34664290 None:intergenic 45.0%
CTCCGGTTAAGTTATTTGGG+AGG + Chr4:34664254-34664273 None:intergenic 45.0%
GACTTGTATGACCCTGATCA+TGG - Chr4:34665548-34665567 MsG0480020128.01.T01:intron 45.0%
GCATGCCATTGTACTTCAGT+TGG + Chr4:34665446-34665465 None:intergenic 45.0%
GTTGGCCCTGCATAATACAT+AGG + Chr4:34665428-34665447 None:intergenic 45.0%
TACAAGTCCCGTTTCTCAGT+TGG + Chr4:34665536-34665555 None:intergenic 45.0%
TCCTCTTGGAGGTTTCACAA+AGG - Chr4:34664778-34664797 MsG0480020128.01.T01:intron 45.0%
TGCTCCATCCTGACATCTAA+CGG + Chr4:34664815-34664834 None:intergenic 45.0%
TGGGTCATCCAACTGAGAAA+CGG - Chr4:34665525-34665544 MsG0480020128.01.T01:intron 45.0%
TGTCCACTCTCACCATGATT+CGG + Chr4:34663845-34663864 None:intergenic 45.0%
TGTCCCACCCTGATTTCAAT+GGG + Chr4:34663922-34663941 None:intergenic 45.0%
TGTTCCGTTAGATGTCAGGA+TGG - Chr4:34664808-34664827 MsG0480020128.01.T01:intron 45.0%
TTGCAGTTCAACCACTTCCA+AGG + Chr4:34665120-34665139 None:intergenic 45.0%
! AGAAAGCTTTAGCACGGCTA+GGG + Chr4:34666098-34666117 None:intergenic 45.0%
! GCTGGAAATTGGCTTATTGG+AGG - Chr4:34664536-34664555 MsG0480020128.01.T01:CDS 45.0%
! GGTCTTGTCAGTTTGATGTC+CGG + Chr4:34664173-34664192 None:intergenic 45.0%
! GTGTTGTCAATCGTGGATTG+CGG + Chr4:34665046-34665065 None:intergenic 45.0%
! TAGAAAGCTTTAGCACGGCT+AGG + Chr4:34666099-34666118 None:intergenic 45.0%
!!! CGCACCTTTTTTCCATGATC+AGG + Chr4:34665563-34665582 None:intergenic 45.0%
ACTCCCCATTGAAATCAGGG+TGG - Chr4:34663915-34663934 MsG0480020128.01.T01:intron 50.0%
AGGATTGCCTTACGTTGAGG+AGG - Chr4:34664502-34664521 MsG0480020128.01.T01:CDS 50.0%
AGTTGGATGACCCATACCAG+TGG + Chr4:34665519-34665538 None:intergenic 50.0%
CAGGATGGAGCAACATAGCA+AGG - Chr4:34664823-34664842 MsG0480020128.01.T01:intron 50.0%
CAGGGCCAACTGAAGTACAA+TGG - Chr4:34665438-34665457 MsG0480020128.01.T01:CDS 50.0%
CCGGAGACAATCTCAGTTCA+TGG - Chr4:34664268-34664287 MsG0480020128.01.T01:intron 50.0%
CTCCCCATTGAAATCAGGGT+GGG - Chr4:34663916-34663935 MsG0480020128.01.T01:intron 50.0%
GCAATGACCTCCTCAACGTA+AGG + Chr4:34664512-34664531 None:intergenic 50.0%
GGGTCATCCAACTGAGAAAC+GGG - Chr4:34665526-34665545 MsG0480020128.01.T01:intron 50.0%
GGTGGCAGCTTATGACACTA+AGG - Chr4:34666050-34666069 MsG0480020128.01.T01:CDS 50.0%
GTCCCACCCTGATTTCAATG+GGG + Chr4:34663921-34663940 None:intergenic 50.0%
GTGTCCCACCCTGATTTCAA+TGG + Chr4:34663923-34663942 None:intergenic 50.0%
GTTAGATTGCGGTTTCGACC+TGG - Chr4:34665236-34665255 MsG0480020128.01.T01:CDS 50.0%
GTTGGATGACCCATACCAGT+GGG + Chr4:34665518-34665537 None:intergenic 50.0%
TCACCGAATCATGGTGAGAG+TGG - Chr4:34663839-34663858 MsG0480020128.01.T01:CDS 50.0%
TCCAGGATTGCCTTACGTTG+AGG - Chr4:34664499-34664518 MsG0480020128.01.T01:CDS 50.0%
TCCTCAACGTAAGGCAATCC+TGG + Chr4:34664503-34664522 None:intergenic 50.0%
AAGGTGCGTAACATGGCTCC+TGG - Chr4:34665575-34665594 MsG0480020128.01.T01:intron 55.0%
ATTGTTGGTCGCGATCCCAC+TGG - Chr4:34665500-34665519 MsG0480020128.01.T01:intron 55.0%
GAGGAGGTCATTGCTCAAGC+TGG - Chr4:34664518-34664537 MsG0480020128.01.T01:CDS 55.0%
ACCTGGGGAACAACTGCTCC+AGG - Chr4:34664482-34664501 MsG0480020128.01.T01:CDS 60.0%
GGTCGCGATCCCACTGGTAT+GGG - Chr4:34665506-34665525 MsG0480020128.01.T01:intron 60.0%
TCCTGGAGCAGTTGTTCCCC+AGG + Chr4:34664486-34664505 None:intergenic 60.0%
TGGTCGCGATCCCACTGGTA+TGG - Chr4:34665505-34665524 MsG0480020128.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 34663785 34666140 34663785 ID=MsG0480020128.01;Name=MsG0480020128.01
Chr4 mRNA 34663785 34666140 34663785 ID=MsG0480020128.01.T01;Parent=MsG0480020128.01;Name=MsG0480020128.01.T01;_AED=0.12;_eAED=0.12;_QI=0|0|0|0.75|0.33|0.25|4|0|301
Chr4 exon 34665992 34666140 34665992 ID=MsG0480020128.01.T01:exon:4388;Parent=MsG0480020128.01.T01
Chr4 exon 34665081 34665486 34665081 ID=MsG0480020128.01.T01:exon:4389;Parent=MsG0480020128.01.T01
Chr4 exon 34664297 34664557 34664297 ID=MsG0480020128.01.T01:exon:4390;Parent=MsG0480020128.01.T01
Chr4 exon 34663785 34663874 34663785 ID=MsG0480020128.01.T01:exon:4391;Parent=MsG0480020128.01.T01
Chr4 CDS 34665992 34666140 34665992 ID=MsG0480020128.01.T01:cds;Parent=MsG0480020128.01.T01
Chr4 CDS 34665081 34665486 34665081 ID=MsG0480020128.01.T01:cds;Parent=MsG0480020128.01.T01
Chr4 CDS 34664297 34664557 34664297 ID=MsG0480020128.01.T01:cds;Parent=MsG0480020128.01.T01
Chr4 CDS 34663785 34663874 34663785 ID=MsG0480020128.01.T01:cds;Parent=MsG0480020128.01.T01
Gene Sequence

>MsG0480020128.01.T01

ATGAATTTGATATCAACTTCATCTGAGAGTGGACAACTGAGTGTTGAAACTTCATCACCGAATCATGGTGAGAGTGGACAATTGAGTGTTGAAAGTTTTTCAATGATTCATGCTGACAGTTCTCAATGTTACTCCCCATTGAAATCAGGCATTGAAATCTACAAGCATAACAAAGAAGAAAGAATAGCTAGAACCTGGGGAACAACTGCTCCAGGATTGCCTTACGTTGAGGAGGTCATTGCTCAAGCTGGAAATTGGCTTATTGGAGGAGATTTGGAAGTGCTAAAACCTATCAAATACAATGATGGTCTTGATAACTACAGGCTCTCTCCCAAACAACTTTGTGAAGAATTCGATTGGCGTAAAGCTGATGCAGTTTTTGCCTTTCAGTTAAGAAATCCGGTTCATAATGGTCATGACTTGTTAATGAACGATACTCGTCAACGTCTATTAGACATGGGTTACAAAAATCCAATTTTATTGCTTCATCCTCTTGGAGGTTTCACAAAGGTTGACGATGTTCCGTTAGATGTCAGGATGGAGCAACATAGCAAGGTCCTAGAATATGGAGTTCTTGATCCAGAGACAACCATTGTTTCTATATTTCCATTGCCTATGTATTATGCAGGGCCAACTGAAGTACAATGGCATGCAAAGTCACGGATAAATGTAGGTGCCAACTTCTATATTGTTGGTCGCGATCCCACTGGTATGGGTCATCCAACTGAGAAACGGGACTTGTATGACCCTGATCATGGAAAAAAGGTGCGTAACATGGCTCCTGGTCTCGAGAAGCTTAACATTTTGCCATTCAGGGTGGCAGCTTATGACACTAAGGTAAACAAGATGGCATTTTTCGACCCTAGCCGTGCTAAAGCTTTCTACACGTTTTTGTGCAGATCCTGA
Protein sequence

>MsG0480020128.01.T01

MNLISTSSESGQLSVETSSPNHGESGQLSVESFSMIHADSSQCYSPLKSGIEIYKHNKEERIARTWGTTAPGLPYVEEVIAQAGNWLIGGDLEVLKPIKYNDGLDNYRLSPKQLCEEFDWRKADAVFAFQLRNPVHNGHDLLMNDTRQRLLDMGYKNPILLLHPLGGFTKVDDVPLDVRMEQHSKVLEYGVLDPETTIVSIFPLPMYYAGPTEVQWHAKSRINVGANFYIVGRDPTGMGHPTEKRDLYDPDHGKKVRNMAPGLEKLNILPFRVAAYDTKVNKMAFFDPSRAKAFYTFLCRS*