Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020294.01.T01 | XP_013455622.1 | 85.135 | 74 | 11 | 0 | 1 | 74 | 1 | 74 | 2.32E-38 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020294.01.T01 | O22059 | 63.83 | 47 | 17 | 0 | 26 | 72 | 36 | 82 | 4.29E-15 | 66.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0480020294.01 | MsG0280010615.01 | PPI |
| MsG0480020294.01 | MsG0280010615.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020294.01.T01 | MTR_4g046737 | 85.135 | 74 | 11 | 0 | 1 | 74 | 1 | 74 | 2.81e-42 | 132 |
| MsG0480020294.01.T01 | MTR_2g088730 | 72.000 | 50 | 14 | 0 | 24 | 73 | 30 | 79 | 1.39e-20 | 77.4 |
| MsG0480020294.01.T01 | MTR_2g023100 | 49.333 | 75 | 35 | 2 | 1 | 73 | 1 | 74 | 6.30e-18 | 70.9 |
| MsG0480020294.01.T01 | MTR_5g009460 | 63.043 | 46 | 17 | 0 | 26 | 71 | 37 | 82 | 2.58e-17 | 69.7 |
| MsG0480020294.01.T01 | MTR_0247s0040 | 60.976 | 41 | 16 | 0 | 28 | 68 | 65 | 105 | 1.98e-13 | 61.6 |
| MsG0480020294.01.T01 | MTR_4g100720 | 63.415 | 41 | 15 | 0 | 28 | 68 | 71 | 111 | 5.66e-12 | 57.8 |
| MsG0480020294.01.T01 | MTR_5g079670 | 56.098 | 41 | 18 | 0 | 28 | 68 | 69 | 109 | 7.17e-12 | 58.2 |
| MsG0480020294.01.T01 | MTR_3g083540 | 57.143 | 42 | 18 | 0 | 27 | 68 | 98 | 139 | 7.34e-12 | 58.9 |
| MsG0480020294.01.T01 | MTR_4g485530 | 56.098 | 41 | 18 | 0 | 28 | 68 | 70 | 110 | 7.55e-12 | 57.8 |
| MsG0480020294.01.T01 | MTR_4g073420 | 56.098 | 41 | 18 | 0 | 28 | 68 | 70 | 110 | 1.51e-11 | 57.8 |
| MsG0480020294.01.T01 | MTR_8g060940 | 56.098 | 41 | 18 | 0 | 28 | 68 | 65 | 105 | 1.55e-11 | 57.8 |
| MsG0480020294.01.T01 | MTR_4g125520 | 58.537 | 41 | 17 | 0 | 28 | 68 | 70 | 110 | 2.09e-11 | 57.8 |
| MsG0480020294.01.T01 | MTR_0197s0010 | 52.273 | 44 | 21 | 0 | 28 | 71 | 59 | 102 | 2.51e-11 | 57.0 |
| MsG0480020294.01.T01 | MTR_8g027345 | 56.098 | 41 | 18 | 0 | 28 | 68 | 70 | 110 | 3.31e-11 | 57.0 |
| MsG0480020294.01.T01 | MTR_7g117730 | 54.545 | 44 | 20 | 0 | 28 | 71 | 70 | 113 | 6.43e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020294.01.T01 | AT2G46410 | 63.830 | 47 | 17 | 0 | 26 | 72 | 36 | 82 | 4.38e-16 | 66.6 |
| MsG0480020294.01.T01 | AT1G01380 | 68.293 | 41 | 13 | 0 | 26 | 66 | 32 | 72 | 1.65e-15 | 64.7 |
| MsG0480020294.01.T01 | AT4G01060 | 63.415 | 41 | 15 | 0 | 26 | 66 | 33 | 73 | 9.77e-14 | 60.1 |
| MsG0480020294.01.T01 | AT4G01060 | 63.415 | 41 | 15 | 0 | 26 | 66 | 30 | 70 | 9.80e-14 | 60.1 |
| MsG0480020294.01.T01 | AT4G01060 | 63.415 | 41 | 15 | 0 | 26 | 66 | 31 | 71 | 9.83e-14 | 60.1 |
| MsG0480020294.01.T01 | AT5G53200 | 56.522 | 46 | 20 | 0 | 26 | 71 | 31 | 76 | 1.58e-13 | 60.5 |
| MsG0480020294.01.T01 | AT5G35550 | 62.500 | 40 | 14 | 1 | 29 | 68 | 74 | 112 | 6.40e-12 | 58.9 |
| MsG0480020294.01.T01 | AT2G30420 | 54.348 | 46 | 21 | 0 | 26 | 71 | 38 | 83 | 8.24e-12 | 56.2 |
| MsG0480020294.01.T01 | AT1G22640 | 56.098 | 41 | 18 | 0 | 28 | 68 | 13 | 53 | 1.26e-11 | 57.4 |
| MsG0480020294.01.T01 | AT4G38620 | 56.098 | 41 | 18 | 0 | 28 | 68 | 70 | 110 | 2.03e-11 | 57.8 |
| MsG0480020294.01.T01 | AT1G22640 | 56.098 | 41 | 18 | 0 | 28 | 68 | 70 | 110 | 2.58e-11 | 57.0 |
| MsG0480020294.01.T01 | AT3G13540 | 58.537 | 41 | 17 | 0 | 28 | 68 | 81 | 121 | 3.36e-11 | 56.6 |
| MsG0480020294.01.T01 | AT5G35550 | 62.500 | 40 | 14 | 1 | 29 | 68 | 77 | 115 | 4.35e-11 | 56.6 |
| MsG0480020294.01.T01 | AT4G09460 | 51.220 | 41 | 20 | 0 | 28 | 68 | 70 | 110 | 5.26e-11 | 56.2 |
| MsG0480020294.01.T01 | AT5G14750 | 50.000 | 44 | 22 | 0 | 28 | 71 | 13 | 56 | 5.28e-11 | 54.7 |
| MsG0480020294.01.T01 | AT5G14750 | 50.000 | 44 | 22 | 0 | 28 | 71 | 13 | 56 | 5.28e-11 | 54.7 |
| MsG0480020294.01.T01 | AT3G49690 | 52.381 | 42 | 20 | 0 | 28 | 69 | 71 | 112 | 7.65e-11 | 56.2 |
Find 13 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGGAAATGCTTATTAAAA+TGG | 0.190572 | 4:+38295227 | MsG0480020294.01.T01:CDS |
| ATCTCTTCTGCTGTTCTTCC+AGG | 0.213845 | 4:-38295583 | None:intergenic |
| AAATGGTTTATAATCTTGTT+GGG | 0.339232 | 4:+38295244 | MsG0480020294.01.T01:CDS |
| TTGATTGCTGGAAGAATTCC+TGG | 0.342209 | 4:+38295565 | MsG0480020294.01.T01:CDS |
| AAAATGGTTTATAATCTTGT+TGG | 0.380870 | 4:+38295243 | MsG0480020294.01.T01:CDS |
| CGTAGGTGGTCATTGATTGC+TGG | 0.396538 | 4:+38295553 | MsG0480020294.01.T01:intron |
| TCAACCTTTGAACCTTGGTC+TGG | 0.418134 | 4:-38295192 | None:intergenic |
| AAAATTCAACCTTTGAACCT+TGG | 0.460780 | 4:-38295197 | None:intergenic |
| TTTATAATCTTGTTGGGGAG+AGG | 0.507013 | 4:+38295250 | MsG0480020294.01.T01:CDS |
| AATGGTTTATAATCTTGTTG+GGG | 0.563787 | 4:+38295245 | MsG0480020294.01.T01:CDS |
| GCAACCAGACCAAGGTTCAA+AGG | 0.564494 | 4:+38295188 | MsG0480020294.01.T01:CDS |
| AATGTTTCAACAGATTCCAA+AGG | 0.606722 | 4:+38295039 | MsG0480020294.01.T01:CDS |
| ACAGATCAGCAACCAGACCA+AGG | 0.650716 | 4:+38295180 | MsG0480020294.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAAAAGTTACAATTTA+AGG | - | Chr4:38295284-38295303 | None:intergenic | 10.0% |
| !! | AAAATGGTTTATAATCTTGT+TGG | + | Chr4:38295243-38295262 | MsG0480020294.01.T01:CDS | 20.0% |
| !! | AAATGGTTTATAATCTTGTT+GGG | + | Chr4:38295244-38295263 | MsG0480020294.01.T01:CDS | 20.0% |
| !! | ATAACATCAATAACAAAGTT+TGG | - | Chr4:38295325-38295344 | None:intergenic | 20.0% |
| !! | ATTTCTCTTAGTCAATAAAA+TGG | - | Chr4:38295148-38295167 | None:intergenic | 20.0% |
| !!! | ATTTTATTGACTAAGAGAAA+TGG | + | Chr4:38295147-38295166 | MsG0480020294.01.T01:intron | 20.0% |
| !!! | TGTCTTTTATAGCTAATTAA+TGG | + | Chr4:38295081-38295100 | MsG0480020294.01.T01:intron | 20.0% |
| ! | AATGGTTTATAATCTTGTTG+GGG | + | Chr4:38295245-38295264 | MsG0480020294.01.T01:CDS | 25.0% |
| ! | AGAGGAAATGCTTATTAAAA+TGG | + | Chr4:38295227-38295246 | MsG0480020294.01.T01:CDS | 25.0% |
| ! | CAGAAGAGATAGAAAAATAT+TGG | + | Chr4:38295596-38295615 | MsG0480020294.01.T01:CDS | 25.0% |
| ! | TAAAAGACATGCATTCTAAA+AGG | - | Chr4:38295070-38295089 | None:intergenic | 25.0% |
| !! | AAAAGACATGCATTCTAAAA+GGG | - | Chr4:38295069-38295088 | None:intergenic | 25.0% |
| !!! | ATCTGTTGAAACATTTTTAG+AGG | - | Chr4:38295034-38295053 | None:intergenic | 25.0% |
| AAAATTCAACCTTTGAACCT+TGG | - | Chr4:38295200-38295219 | None:intergenic | 30.0% | |
| AATGTTTCAACAGATTCCAA+AGG | + | Chr4:38295039-38295058 | MsG0480020294.01.T01:CDS | 30.0% | |
| GTCTAGTGTTTGTGTTTATT+TGG | - | Chr4:38295361-38295380 | None:intergenic | 30.0% | |
| ! | ATGAATGTGTATTTTCTCGT+AGG | + | Chr4:38295536-38295555 | MsG0480020294.01.T01:intron | 30.0% |
| ! | ATTCTAAAAGGGTATACCTT+TGG | - | Chr4:38295058-38295077 | None:intergenic | 30.0% |
| ! | TTTGGAATGAAAGTGAAAAG+AGG | - | Chr4:38295307-38295326 | None:intergenic | 30.0% |
| !! | GGTTGAATTTTCTAAAGAAG+AGG | + | Chr4:38295209-38295228 | MsG0480020294.01.T01:CDS | 30.0% |
| TTTATAATCTTGTTGGGGAG+AGG | + | Chr4:38295250-38295269 | MsG0480020294.01.T01:CDS | 35.0% | |
| ! | AATGTGTATTTTCTCGTAGG+TGG | + | Chr4:38295539-38295558 | MsG0480020294.01.T01:intron | 35.0% |
| AACACTGCATGTATTCGTGT+TGG | + | Chr4:38295416-38295435 | MsG0480020294.01.T01:intron | 40.0% | |
| TTGATTGCTGGAAGAATTCC+TGG | + | Chr4:38295565-38295584 | MsG0480020294.01.T01:CDS | 40.0% | |
| ! | AAACCTTCGATCTAGAGTGT+CGG | + | Chr4:38295465-38295484 | MsG0480020294.01.T01:intron | 40.0% |
| ATCTCTTCTGCTGTTCTTCC+AGG | - | Chr4:38295586-38295605 | None:intergenic | 45.0% | |
| CTCTAGATCGAAGGTTTGTC+TGG | - | Chr4:38295462-38295481 | None:intergenic | 45.0% | |
| TCAACCTTTGAACCTTGGTC+TGG | - | Chr4:38295195-38295214 | None:intergenic | 45.0% | |
| ACACCGACACTCTAGATCGA+AGG | - | Chr4:38295471-38295490 | None:intergenic | 50.0% | |
| ACAGATCAGCAACCAGACCA+AGG | + | Chr4:38295180-38295199 | MsG0480020294.01.T01:intron | 50.0% | |
| GCAACCAGACCAAGGTTCAA+AGG | + | Chr4:38295188-38295207 | MsG0480020294.01.T01:CDS | 50.0% | |
| ! | CGTAGGTGGTCATTGATTGC+TGG | + | Chr4:38295553-38295572 | MsG0480020294.01.T01:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 38295012 | 38295645 | 38295012 | ID=MsG0480020294.01;Name=MsG0480020294.01 |
| Chr4 | mRNA | 38295012 | 38295645 | 38295012 | ID=MsG0480020294.01.T01;Parent=MsG0480020294.01;Name=MsG0480020294.01.T01;_AED=0.38;_eAED=0.39;_QI=0|0|0|1|1|1|3|0|74 |
| Chr4 | exon | 38295012 | 38295060 | 38295012 | ID=MsG0480020294.01.T01:exon:3657;Parent=MsG0480020294.01.T01 |
| Chr4 | exon | 38295184 | 38295271 | 38295184 | ID=MsG0480020294.01.T01:exon:3658;Parent=MsG0480020294.01.T01 |
| Chr4 | exon | 38295558 | 38295645 | 38295558 | ID=MsG0480020294.01.T01:exon:3659;Parent=MsG0480020294.01.T01 |
| Chr4 | CDS | 38295012 | 38295060 | 38295012 | ID=MsG0480020294.01.T01:cds;Parent=MsG0480020294.01.T01 |
| Chr4 | CDS | 38295184 | 38295271 | 38295184 | ID=MsG0480020294.01.T01:cds;Parent=MsG0480020294.01.T01 |
| Chr4 | CDS | 38295558 | 38295645 | 38295558 | ID=MsG0480020294.01.T01:cds;Parent=MsG0480020294.01.T01 |
| Gene Sequence |
| Protein sequence |
