AlfalfaGEDB | MsG0480020645.01

M. sativa cultivar ZhongmuNo.1 / MsG0480020645.01

KEGG: K03094
GO: GO:0006511
PFAM: PF01466 PF03931

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020645.01.T01	XP_003612227.1	96.774	155	5	0	1	155	1	155	4.36E-103	301

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020645.01.T01	Q9FHW7	74.269	171	28	1	1	155	1	171	1.71E-79	235

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020645.01.T01	Q155U2	96.774	155	5	0	1	155	1	155	2.08e-103	301

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0480020645.01	MsG0680030747.01	PPI
MsG0280007117.01	MsG0480020645.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020645.01.T01	MTR_5g022730	96.774	155	5	0	1	155	1	155	5.28e-107	301
MsG0480020645.01.T01	MTR_5g022710	93.548	155	10	0	1	155	1	155	5.02e-96	274
MsG0480020645.01.T01	MTR_7g021400	83.766	154	25	0	2	155	3	156	4.24e-86	249
MsG0480020645.01.T01	MTR_7g094150	83.974	156	24	1	1	155	1	156	1.33e-84	245
MsG0480020645.01.T01	MTR_7g094170	82.468	154	27	0	2	155	3	156	1.63e-84	245
MsG0480020645.01.T01	MTR_4g032400	76.129	155	36	1	2	155	9	163	2.18e-80	235
MsG0480020645.01.T01	MTR_7g094120	73.718	156	39	1	2	155	4	159	3.26e-79	232
MsG0480020645.01.T01	MTR_7g021380	79.870	154	31	0	2	155	3	156	5.04e-79	231
MsG0480020645.01.T01	MTR_3g110048	73.077	156	40	1	2	155	3	158	2.05e-78	229
MsG0480020645.01.T01	MTR_4g032450	72.258	155	42	1	2	155	21	175	2.90e-78	230
MsG0480020645.01.T01	MTR_5g085030	70.968	155	44	1	1	154	1	155	6.45e-74	218
MsG0480020645.01.T01	MTR_7g094280	71.519	158	42	2	1	155	1	158	1.30e-73	218
MsG0480020645.01.T01	MTR_8g056060	72.258	155	42	1	2	155	3	157	1.58e-73	217
MsG0480020645.01.T01	MTR_8g468480	68.590	156	48	1	1	155	1	156	9.14e-73	215
MsG0480020645.01.T01	MTR_7g094310	68.354	158	37	3	1	155	1	148	6.92e-66	197
MsG0480020645.01.T01	MTR_4g032460	69.872	156	40	2	1	155	1	150	2.46e-65	196
MsG0480020645.01.T01	MTR_4g032510	66.452	155	44	3	3	155	5	153	1.23e-64	194
MsG0480020645.01.T01	MTR_4g032500	64.516	155	48	2	2	155	4	152	9.85e-64	192
MsG0480020645.01.T01	MTR_4g033455	59.091	154	63	0	2	155	7	160	1.26e-60	184
MsG0480020645.01.T01	MTR_5g082340	64.744	156	43	2	1	155	1	145	5.22e-60	182
MsG0480020645.01.T01	MTR_4g017860	53.333	195	48	3	4	155	26	220	1.36e-58	181
MsG0480020645.01.T01	MTR_1g099870	82.353	102	16	1	56	155	2	103	1.37e-55	170
MsG0480020645.01.T01	MTR_4g033460	59.420	138	56	0	2	139	7	144	2.81e-54	171
MsG0480020645.01.T01	MTR_4g033460	58.065	93	31	1	40	132	161	245	2.60e-28	105
MsG0480020645.01.T01	MTR_4g021390	56.494	154	64	1	2	155	5	155	1.37e-52	164
MsG0480020645.01.T01	MTR_7g094320	56.688	157	26	2	1	155	1	117	2.31e-47	149
MsG0480020645.01.T01	MTR_8g089730	67.480	123	23	1	33	155	1	106	7.89e-46	145
MsG0480020645.01.T01	MTR_4g032420	60.630	127	35	3	30	155	38	150	3.22e-45	145
MsG0480020645.01.T01	MTR_4g032470	49.315	146	30	2	11	155	32	134	8.73e-41	134
MsG0480020645.01.T01	MTR_7g006370	53.571	140	56	2	2	141	5	135	1.57e-40	133
MsG0480020645.01.T01	MTR_4g131980	62.500	120	39	3	1	118	1	116	8.64e-38	126
MsG0480020645.01.T01	MTR_4g127240	65.049	103	30	4	39	139	26	124	1.81e-37	125
MsG0480020645.01.T01	MTR_7g094410	49.682	157	31	3	2	113	3	156	5.05e-37	125
MsG0480020645.01.T01	MTR_7g094360	44.767	172	41	3	1	118	1	172	3.62e-35	121
MsG0480020645.01.T01	MTR_8g056090	48.052	154	47	6	2	153	3	125	4.06e-33	114
MsG0480020645.01.T01	MTR_4g119422	47.260	146	70	2	2	145	228	368	3.69e-31	115
MsG0480020645.01.T01	MTR_7g071200	71.014	69	19	1	88	155	4	72	2.72e-28	100
MsG0480020645.01.T01	MTR_1g112590	37.419	155	81	2	7	145	1	155	3.07e-28	102
MsG0480020645.01.T01	MTR_4g032520	44.056	143	38	4	29	153	2	120	4.04e-25	94.0
MsG0480020645.01.T01	MTR_2g066010	39.073	151	80	4	7	154	20	161	1.08e-23	92.0
MsG0480020645.01.T01	MTR_7g094350	54.444	90	28	1	2	91	3	79	1.79e-23	91.3
MsG0480020645.01.T01	MTR_7g094350	51.685	89	39	3	47	134	102	187	5.39e-19	79.7
MsG0480020645.01.T01	MTR_2g065720	38.411	151	81	4	7	154	37	178	1.10e-22	89.7
MsG0480020645.01.T01	MTR_4g032540	63.492	63	22	1	89	150	70	132	2.11e-20	81.6
MsG0480020645.01.T01	MTR_2g018980	33.987	153	82	3	7	155	3	140	6.92e-19	78.2
MsG0480020645.01.T01	MTR_5g096950	34.615	156	83	3	3	154	2	142	7.49e-19	78.2
MsG0480020645.01.T01	MTR_1g112570	35.252	139	65	4	7	127	1	132	2.84e-18	79.3
MsG0480020645.01.T01	MTR_1g042510	33.333	156	66	4	7	145	1	135	5.79e-18	78.6
MsG0480020645.01.T01	MTR_6g053620	34.722	144	84	4	7	145	17	155	4.04e-17	77.0
MsG0480020645.01.T01	MTR_6g053620	36.111	144	82	5	7	145	17	155	4.29e-17	77.0
MsG0480020645.01.T01	MTR_8g007195	35.000	140	79	4	9	142	13	146	5.01e-17	76.6
MsG0480020645.01.T01	MTR_5g078680	32.609	138	70	3	7	127	1	132	6.30e-17	75.5
MsG0480020645.01.T01	MTR_7g077810	34.722	144	84	4	7	145	18	156	3.14e-16	74.3
MsG0480020645.01.T01	MTR_8g007185	39.815	108	57	3	41	146	50	151	5.17e-16	73.6
MsG0480020645.01.T01	MTR_8g007205	39.815	108	57	3	41	146	50	151	5.27e-16	73.6
MsG0480020645.01.T01	MTR_2g100830	30.986	142	88	3	9	145	13	149	7.21e-14	67.8
MsG0480020645.01.T01	MTR_2g100825	32.168	143	88	4	7	145	11	148	7.73e-14	67.8
MsG0480020645.01.T01	MTR_8g030670	35.398	113	64	3	37	148	55	159	1.03e-13	67.4
MsG0480020645.01.T01	MTR_4g131910	38.393	112	59	2	4	111	133	238	3.33e-13	65.1
MsG0480020645.01.T01	MTR_7g036780	29.710	138	87	4	2	134	4	136	2.09e-12	62.8
MsG0480020645.01.T01	MTR_3g061700	35.870	92	52	2	52	142	1	86	4.25e-12	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020645.01.T01	AT5G42190	74.269	171	28	1	1	155	1	171	1.74e-80	235
MsG0480020645.01.T01	AT1G75950	76.101	159	32	1	3	155	2	160	9.78e-79	231
MsG0480020645.01.T01	AT1G20140	69.325	163	42	2	1	155	1	163	4.02e-77	226
MsG0480020645.01.T01	AT2G25700	67.073	164	44	3	1	155	1	163	2.21e-73	217
MsG0480020645.01.T01	AT4G34210	68.182	154	45	2	3	155	2	152	5.33e-71	211
MsG0480020645.01.T01	AT4G34470	67.532	154	46	2	3	155	2	152	2.64e-70	209
MsG0480020645.01.T01	AT3G21860	65.161	155	48	3	3	155	2	152	1.11e-63	192
MsG0480020645.01.T01	AT3G21850	61.935	155	54	3	3	155	2	153	5.83e-61	185
MsG0480020645.01.T01	AT3G60010	61.688	154	56	2	4	155	2	154	9.85e-61	185
MsG0480020645.01.T01	AT2G03170	61.688	154	52	3	3	155	2	149	2.74e-58	178
MsG0480020645.01.T01	AT2G03190	54.819	166	62	3	3	155	2	167	4.84e-55	171
MsG0480020645.01.T01	AT3G60020	54.430	158	60	3	4	155	2	153	7.90e-53	165
MsG0480020645.01.T01	AT2G03160	48.148	189	62	3	3	155	2	190	5.32e-52	164
MsG0480020645.01.T01	AT2G20160	54.902	153	63	2	3	154	2	149	4.19e-51	160
MsG0480020645.01.T01	AT3G25650	56.024	166	60	5	3	155	2	167	5.85e-50	158
MsG0480020645.01.T01	AT3G21830	54.194	155	65	3	3	155	2	152	1.57e-49	156
MsG0480020645.01.T01	AT1G10230	55.484	155	66	2	3	154	2	156	4.59e-48	153
MsG0480020645.01.T01	AT3G21840	56.667	120	46	3	3	120	2	117	2.75e-34	117
MsG0480020645.01.T01	AT3G53060	62.791	86	25	3	28	112	1	80	6.50e-29	102
MsG0480020645.01.T01	AT3G61415	33.333	144	86	4	7	145	18	156	9.47e-15	70.5
MsG0480020645.01.T01	AT3G61415	33.333	144	86	4	7	145	18	156	9.77e-15	70.5
MsG0480020645.01.T01	AT2G45950	31.250	144	89	4	7	145	18	156	2.30e-14	69.3
MsG0480020645.01.T01	AT2G45950	31.250	144	89	4	7	145	18	156	2.30e-14	69.3
MsG0480020645.01.T01	AT2G45950	31.250	144	89	4	7	145	18	156	2.30e-14	69.3
MsG0480020645.01.T01	AT2G45950	31.250	144	89	4	7	145	18	156	2.36e-14	69.3
MsG0480020645.01.T01	AT2G45950	31.250	144	89	4	7	145	18	156	2.36e-14	69.3

Find 36 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAAGAATGATTTCTCTTTAG+AGG	0.242463	4:+45288380	MsG0480020645.01.T01:CDS
AAGTTCTTGCGAATCTCTTC+AGG	0.302348	4:-45288351	None:intergenic
TAACGTTACAAGCAAGATCT+TGG	0.400481	4:+45288116	MsG0480020645.01.T01:CDS
TGATTTCTCTTTAGAGGAAG+AGG	0.431147	4:+45288386	MsG0480020645.01.T01:CDS
TGATCATGTCTGCCACAGTC+TGG	0.431834	4:-45288317	None:intergenic
GGAAGAGGAGGAAGTTCGTA+GGG	0.441516	4:+45288401	MsG0480020645.01.T01:CDS
CAAGTTCGTGAAGGTTGATC+AGG	0.447990	4:+45288227	MsG0480020645.01.T01:CDS
CTGATCAACCTTCACGAACT+TGG	0.453869	4:-45288226	None:intergenic
AAGATCTTGCTTGTAACGTT+AGG	0.455592	4:-45288114	None:intergenic
AGGAAGAGGAGGAAGTTCGT+AGG	0.467294	4:+45288400	MsG0480020645.01.T01:CDS
TTTGATTATCAAGAACCTGT+TGG	0.482150	4:+45288284	MsG0480020645.01.T01:CDS
GGTTGATGAAGCGGTTGCCT+TGG	0.491356	4:+45288032	MsG0480020645.01.T01:CDS
GACTTAAGCTCATCCTCGTT+AGG	0.508059	4:-45288195	None:intergenic
TTGCTTGTAACGTTAGGAAG+AGG	0.510236	4:-45288108	None:intergenic
TTCCGACTGTGAGACCTTCG+AGG	0.512798	4:+45288011	MsG0480020645.01.T01:CDS
CGAGGATGAGCTTAAGTCCT+GGG	0.519699	4:+45288200	MsG0480020645.01.T01:CDS
TGCTTGTAACGTTAGGAAGA+GGG	0.532652	4:-45288107	None:intergenic
TCTGGCATGTAAGATCCAAC+AGG	0.534781	4:-45288299	None:intergenic
CTTCACGAACTTGGAATCCC+AGG	0.536138	4:-45288217	None:intergenic
ATAATCATTCAAATGCCCAT+TGG	0.538622	4:-45288428	None:intergenic
GACTGTGGCAGACATGATCA+AGG	0.557841	4:+45288320	MsG0480020645.01.T01:CDS
AGTTCGTAGGGAAAACCAAT+GGG	0.562143	4:+45288413	MsG0480020645.01.T01:CDS
AAGTTCGTAGGGAAAACCAA+TGG	0.564773	4:+45288412	MsG0480020645.01.T01:CDS
GACCTTCGAGGTTGATGAAG+CGG	0.566617	4:+45288023	MsG0480020645.01.T01:CDS
ACGAGGATGAGCTTAAGTCC+TGG	0.579392	4:+45288199	MsG0480020645.01.T01:CDS
TTTCTCTTTAGAGGAAGAGG+AGG	0.582316	4:+45288389	MsG0480020645.01.T01:CDS
CTTGATCGTCTGAGATTCCA+AGG	0.584428	4:-45288049	None:intergenic
GAAGACGATTGCGCTGACAG+TGG	0.597220	4:+45288081	MsG0480020645.01.T01:CDS
AACCGCTTCATCAACCTCGA+AGG	0.615888	4:-45288025	None:intergenic
CTGGGATTCCAAGTTCGTGA+AGG	0.623378	4:+45288218	MsG0480020645.01.T01:CDS
ACTGTGGCAGACATGATCAA+GGG	0.624146	4:+45288321	MsG0480020645.01.T01:CDS
TACAAGCAAGATCTTGGCGA+AGG	0.627734	4:+45288122	MsG0480020645.01.T01:CDS
AACCTCGAAGGTCTCACAGT+CGG	0.635769	4:-45288013	None:intergenic
CTGTGGCAGACATGATCAAG+GGG	0.638852	4:+45288322	MsG0480020645.01.T01:CDS
TGCTGAAGAGAAGCCTAACG+AGG	0.662168	4:+45288182	MsG0480020645.01.T01:CDS
GGATCTTACATGCCAGACTG+TGG	0.714219	4:+45288305	MsG0480020645.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TAAGAATGATTTCTCTTTAG+AGG	+	Chr4:45288380-45288399	MsG0480020645.01.T01:CDS	25.0%
	TTTGATTATCAAGAACCTGT+TGG	+	Chr4:45288284-45288303	MsG0480020645.01.T01:CDS	30.0%
!!	TACACTTTTTGATCTCATCT+TGG	+	Chr4:45288251-45288270	MsG0480020645.01.T01:CDS	30.0%
	AAGATCTTGCTTGTAACGTT+AGG	-	Chr4:45288117-45288136	None:intergenic	35.0%
	TAACGTTACAAGCAAGATCT+TGG	+	Chr4:45288116-45288135	MsG0480020645.01.T01:CDS	35.0%
!	TGATTTCTCTTTAGAGGAAG+AGG	+	Chr4:45288386-45288405	MsG0480020645.01.T01:CDS	35.0%
	AAGTTCGTAGGGAAAACCAA+TGG	+	Chr4:45288412-45288431	MsG0480020645.01.T01:CDS	40.0%
	AAGTTCTTGCGAATCTCTTC+AGG	-	Chr4:45288354-45288373	None:intergenic	40.0%
	AGTTCGTAGGGAAAACCAAT+GGG	+	Chr4:45288413-45288432	MsG0480020645.01.T01:CDS	40.0%
	TGCTTGTAACGTTAGGAAGA+GGG	-	Chr4:45288110-45288129	None:intergenic	40.0%
	TTGCTTGTAACGTTAGGAAG+AGG	-	Chr4:45288111-45288130	None:intergenic	40.0%
!	TTTCTCTTTAGAGGAAGAGG+AGG	+	Chr4:45288389-45288408	MsG0480020645.01.T01:CDS	40.0%
	ACTGTGGCAGACATGATCAA+GGG	+	Chr4:45288321-45288340	MsG0480020645.01.T01:CDS	45.0%
	CTGATCAACCTTCACGAACT+TGG	-	Chr4:45288229-45288248	None:intergenic	45.0%
	GACTTAAGCTCATCCTCGTT+AGG	-	Chr4:45288198-45288217	None:intergenic	45.0%
	TACAAGCAAGATCTTGGCGA+AGG	+	Chr4:45288122-45288141	MsG0480020645.01.T01:CDS	45.0%
	TCTGGCATGTAAGATCCAAC+AGG	-	Chr4:45288302-45288321	None:intergenic	45.0%
!	CAAGTTCGTGAAGGTTGATC+AGG	+	Chr4:45288227-45288246	MsG0480020645.01.T01:CDS	45.0%
!	CTTGATCGTCTGAGATTCCA+AGG	-	Chr4:45288052-45288071	None:intergenic	45.0%
	AACCGCTTCATCAACCTCGA+AGG	-	Chr4:45288028-45288047	None:intergenic	50.0%
	AACCTCGAAGGTCTCACAGT+CGG	-	Chr4:45288016-45288035	None:intergenic	50.0%
	ACGAGGATGAGCTTAAGTCC+TGG	+	Chr4:45288199-45288218	MsG0480020645.01.T01:CDS	50.0%
	AGGAAGAGGAGGAAGTTCGT+AGG	+	Chr4:45288400-45288419	MsG0480020645.01.T01:CDS	50.0%
	CGAGGATGAGCTTAAGTCCT+GGG	+	Chr4:45288200-45288219	MsG0480020645.01.T01:CDS	50.0%
	CTGGGATTCCAAGTTCGTGA+AGG	+	Chr4:45288218-45288237	MsG0480020645.01.T01:CDS	50.0%
	CTGTGGCAGACATGATCAAG+GGG	+	Chr4:45288322-45288341	MsG0480020645.01.T01:CDS	50.0%
	CTTCACGAACTTGGAATCCC+AGG	-	Chr4:45288220-45288239	None:intergenic	50.0%
	GACTGTGGCAGACATGATCA+AGG	+	Chr4:45288320-45288339	MsG0480020645.01.T01:CDS	50.0%
	GGAAGAGGAGGAAGTTCGTA+GGG	+	Chr4:45288401-45288420	MsG0480020645.01.T01:CDS	50.0%
	GGATCTTACATGCCAGACTG+TGG	+	Chr4:45288305-45288324	MsG0480020645.01.T01:CDS	50.0%
	TGATCATGTCTGCCACAGTC+TGG	-	Chr4:45288320-45288339	None:intergenic	50.0%
	TGCTGAAGAGAAGCCTAACG+AGG	+	Chr4:45288182-45288201	MsG0480020645.01.T01:CDS	50.0%
!!	GACCTTCGAGGTTGATGAAG+CGG	+	Chr4:45288023-45288042	MsG0480020645.01.T01:CDS	50.0%
	TTCCGACTGTGAGACCTTCG+AGG	+	Chr4:45288011-45288030	MsG0480020645.01.T01:CDS	55.0%
!	GAAGACGATTGCGCTGACAG+TGG	+	Chr4:45288081-45288100	MsG0480020645.01.T01:CDS	55.0%
!!	GGTTGATGAAGCGGTTGCCT+TGG	+	Chr4:45288032-45288051	MsG0480020645.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	45287979	45288446	45287979	ID=MsG0480020645.01;Name=MsG0480020645.01
Chr4	mRNA	45287979	45288446	45287979	ID=MsG0480020645.01.T01;Parent=MsG0480020645.01;Name=MsG0480020645.01.T01;_AED=0.35;_eAED=0.35;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|155
Chr4	exon	45287979	45288446	45287979	ID=MsG0480020645.01.T01:exon:1840;Parent=MsG0480020645.01.T01
Chr4	CDS	45287979	45288446	45287979	ID=MsG0480020645.01.T01:cds;Parent=MsG0480020645.01.T01

Gene Sequence

>MsG0480020645.01.T01

ATGTCTTCAACAAGAAAGATCACTCTCAAGAGTTCCGACTGTGAGACCTTCGAGGTTGATGAAGCGGTTGCCTTGGAATCTCAGACGATCAAGCATATGATCGAAGACGATTGCGCTGACAGTGGAATCCCTCTTCCTAACGTTACAAGCAAGATCTTGGCGAAGGTGATTGAGTATTGCAAGAAGCATGTTGATGCTGCTGCTGCTGAAGAGAAGCCTAACGAGGATGAGCTTAAGTCCTGGGATTCCAAGTTCGTGAAGGTTGATCAGGCTACACTTTTTGATCTCATCTTGGCTGCTAATTATTTGATTATCAAGAACCTGTTGGATCTTACATGCCAGACTGTGGCAGACATGATCAAGGGGAAGACACCTGAAGAGATTCGCAAGAACTTCAACATTAAGAATGATTTCTCTTTAGAGGAAGAGGAGGAAGTTCGTAGGGAAAACCAATGGGCATTTGAATGA

Protein sequence

>MsG0480020645.01.T01

MSSTRKITLKSSDCETFEVDEAVALESQTIKHMIEDDCADSGIPLPNVTSKILAKVIEYCKKHVDAAAAEEKPNEDELKSWDSKFVKVDQATLFDLILAANYLIIKNLLDLTCQTVADMIKGKTPEEIRKNFNIKNDFSLEEEEEVRRENQWAFE*