Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020692.01.T01 | XP_003606242.2 | 93.694 | 111 | 7 | 0 | 1 | 111 | 1 | 111 | 1.84E-67 | 210 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020692.01.T01 | G7JKX9 | 93.694 | 111 | 7 | 0 | 1 | 111 | 1 | 111 | 8.80e-68 | 210 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480020692.01 | MsG0480023832.01 | 0.823076 | 1.683159e-53 | 1.434612e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020692.01.T01 | MTR_4g055130 | 93.694 | 111 | 7 | 0 | 1 | 111 | 1 | 111 | 2.23e-71 | 210 |
| MsG0480020692.01.T01 | MTR_4g053310 | 69.925 | 133 | 37 | 2 | 3 | 135 | 1 | 130 | 2.24e-58 | 177 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020692.01.T01 | AT3G44716 | 41.509 | 53 | 31 | 0 | 45 | 97 | 63 | 115 | 1.91e-11 | 58.2 |
| MsG0480020692.01.T01 | AT3G44716 | 40.741 | 54 | 32 | 0 | 45 | 98 | 63 | 116 | 2.42e-11 | 57.8 |
Find 63 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTATATTGAAGTTGAATTT+TGG | 0.142755 | 4:+46346307 | MsG0480020692.01.T01:three_prime_UTR |
| CAAACTACCAGCAGAATTTA+TGG | 0.154751 | 4:-46345879 | None:intergenic |
| CTTCTATATCTTCTCAATTA+AGG | 0.178570 | 4:+46345622 | MsG0480020692.01.T01:CDS |
| AATCTCTCTTAACATTTCTA+TGG | 0.180695 | 4:-46346781 | None:intergenic |
| TGTGACTTGGCATCAGTTTA+TGG | 0.211187 | 4:-46346557 | None:intergenic |
| CACAGTACTTTGTGTGATTA+TGG | 0.216686 | 4:-46345560 | None:intergenic |
| CTCCTATTCAATCTCATATT+AGG | 0.227354 | 4:+46345522 | MsG0480020692.01.T01:CDS |
| TCACACCCTTTGCAAAATTC+TGG | 0.269512 | 4:-46345672 | None:intergenic |
| CAAAATTCTGGTGCATGAAT+TGG | 0.270407 | 4:-46345660 | None:intergenic |
| AGTAGTATTAGCAATGGTTT+TGG | 0.282516 | 4:-46346503 | None:intergenic |
| AGGCACTTACTGCATTCTTT+TGG | 0.293838 | 4:-46345741 | None:intergenic |
| AACACACTGCTCAAAATATA+TGG | 0.327340 | 4:+46346279 | MsG0480020692.01.T01:three_prime_UTR |
| TTCATCAATATTAATAATGA+TGG | 0.354998 | 4:+46346526 | MsG0480020692.01.T01:three_prime_UTR |
| ACACTTCAAGCCAGCATTAC+AGG | 0.368306 | 4:-46345709 | None:intergenic |
| CCAGCATTACAGGTTCCATT+AGG | 0.376854 | 4:-46345699 | None:intergenic |
| GGATTGGAGCCTGGAGGGCT+AGG | 0.377789 | 4:-46346639 | None:intergenic |
| CGCATCTGGAAACGCAGTTA+TGG | 0.392472 | 4:-46346197 | None:intergenic |
| TGTATTGATATTGGAGAGAA+AGG | 0.398388 | 4:+46345453 | MsG0480020692.01.T01:five_prime_UTR |
| ATTACTAATAATTCAGTCCT+TGG | 0.403021 | 4:-46345972 | None:intergenic |
| AGAAATGCAACCAACGCATC+TGG | 0.416080 | 4:-46346211 | None:intergenic |
| GAAATTAAATTGTGTCACTA+TGG | 0.433330 | 4:+46346691 | MsG0480020692.01.T01:three_prime_UTR |
| TTTGTTACTTGTGAAGGGAT+TGG | 0.435881 | 4:-46346655 | None:intergenic |
| ATGTAAGAGTTTAATATGTA+TGG | 0.438562 | 4:+46346239 | MsG0480020692.01.T01:three_prime_UTR |
| TGAATCTTTGTTACTTGTGA+AGG | 0.445303 | 4:-46346661 | None:intergenic |
| CTTGTGAAGGGATTGGAGCC+TGG | 0.446417 | 4:-46346648 | None:intergenic |
| GGAGTAAGAATTGAATAAGA+TGG | 0.451938 | 4:-46345503 | None:intergenic |
| TCATCAATATTAATAATGAT+GGG | 0.453892 | 4:+46346527 | MsG0480020692.01.T01:three_prime_UTR |
| AGTTGTTGAATCGCAACGTT+GGG | 0.458015 | 4:+46346118 | MsG0480020692.01.T01:three_prime_UTR |
| ATCTCTCTTAACATTTCTAT+GGG | 0.458184 | 4:-46346780 | None:intergenic |
| AAAGGGTGTGAAAATCCTAA+TGG | 0.489463 | 4:+46345684 | MsG0480020692.01.T01:CDS |
| CCTAATGGAACCTGTAATGC+TGG | 0.493266 | 4:+46345699 | MsG0480020692.01.T01:CDS |
| TTGAGAATTCCTAGCCCTCC+AGG | 0.498033 | 4:+46346630 | MsG0480020692.01.T01:three_prime_UTR |
| AACCTAATATGAGATTGAAT+AGG | 0.506598 | 4:-46345524 | None:intergenic |
| GAATCTTTGTTACTTGTGAA+GGG | 0.522937 | 4:-46346660 | None:intergenic |
| TTTAAATCGTGATAAAGAAA+CGG | 0.526688 | 4:+46345997 | MsG0480020692.01.T01:three_prime_UTR |
| TCAGAAAGCAATTTATGAAG+AGG | 0.531148 | 4:+46346586 | MsG0480020692.01.T01:three_prime_UTR |
| TATCCTCCTGTGAACATGTA+TGG | 0.543871 | 4:+46346348 | MsG0480020692.01.T01:three_prime_UTR |
| GATAAGGTCATCTCCAAGGT+TGG | 0.548075 | 4:+46345854 | MsG0480020692.01.T01:CDS |
| GAATATTCAAACAATAGAGC+AGG | 0.550299 | 4:+46345426 | MsG0480020692.01.T01:five_prime_UTR |
| AACTGCGTTTCCAGATGCGT+TGG | 0.552961 | 4:+46346201 | MsG0480020692.01.T01:three_prime_UTR |
| GTTGGATCCATAAATTCTGC+TGG | 0.554548 | 4:+46345872 | MsG0480020692.01.T01:CDS |
| GTATTGATATTGGAGAGAAA+GGG | 0.554588 | 4:+46345454 | MsG0480020692.01.T01:five_prime_UTR |
| GATGAAAGTAGTATTAGCAA+TGG | 0.555804 | 4:-46346509 | None:intergenic |
| TTAATATGTATGGCACAAAA+CGG | 0.559829 | 4:+46346249 | MsG0480020692.01.T01:three_prime_UTR |
| AGAATTTATGGATCCAACCT+TGG | 0.569066 | 4:-46345867 | None:intergenic |
| CATCAATATTAATAATGATG+GGG | 0.574094 | 4:+46346528 | MsG0480020692.01.T01:three_prime_UTR |
| TGAAGGGATTGGAGCCTGGA+GGG | 0.575815 | 4:-46346644 | None:intergenic |
| TTTCTGATAGAGTTGTGACT+TGG | 0.578399 | 4:-46346570 | None:intergenic |
| GACTTAGACATGCATGAGGA+AGG | 0.593311 | 4:-46345937 | None:intergenic |
| AAGAAACCATACATGTTCAC+AGG | 0.594129 | 4:-46346354 | None:intergenic |
| AAGTCTAGAAAGCTTATCCA+AGG | 0.596805 | 4:+46345955 | MsG0480020692.01.T01:CDS |
| ATAATCACACAAAGTACTGT+GGG | 0.609420 | 4:+46345562 | MsG0480020692.01.T01:CDS |
| CATAATCACACAAAGTACTG+TGG | 0.626347 | 4:+46345561 | MsG0480020692.01.T01:CDS |
| GTGAAGGGATTGGAGCCTGG+AGG | 0.626615 | 4:-46346645 | None:intergenic |
| AGCAGATAAGGTCATCTCCA+AGG | 0.631454 | 4:+46345850 | MsG0480020692.01.T01:intron |
| ATAAGATGGAGTTTCTTGAG+TGG | 0.641609 | 4:-46345489 | None:intergenic |
| TAGTTGTTGAATCGCAACGT+TGG | 0.646150 | 4:+46346117 | MsG0480020692.01.T01:three_prime_UTR |
| TACTTTGTGTGATTATGGTG+AGG | 0.661995 | 4:-46345555 | None:intergenic |
| TCTAGACTTAGACATGCATG+AGG | 0.680915 | 4:-46345941 | None:intergenic |
| AAACCATACATGTTCACAGG+AGG | 0.681643 | 4:-46346351 | None:intergenic |
| TAGTTCGTAGTTATAAATCG+CGG | 0.703858 | 4:+46346173 | MsG0480020692.01.T01:three_prime_UTR |
| GGTAGATCTAATGATTGATG+TGG | 0.711190 | 4:+46346427 | MsG0480020692.01.T01:three_prime_UTR |
| ATTCTCGAAACTTTACACAG+CGG | 0.774312 | 4:+46346406 | MsG0480020692.01.T01:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAACTTTATATGATTA+AGG | - | Chr4:46345764-46345783 | None:intergenic | 10.0% |
| !!! | AATAATAACAGTTTTAAAAA+GGG | - | Chr4:46346041-46346060 | None:intergenic | 10.0% |
| !!! | TAATAATAACAGTTTTAAAA+AGG | - | Chr4:46346042-46346061 | None:intergenic | 10.0% |
| !! | TCATCAATATTAATAATGAT+GGG | + | Chr4:46346527-46346546 | MsG0480020692.01.T01:three_prime_UTR | 15.0% |
| !! | TTCATCAATATTAATAATGA+TGG | + | Chr4:46346526-46346545 | MsG0480020692.01.T01:three_prime_UTR | 15.0% |
| !! | ATGTAAGAGTTTAATATGTA+TGG | + | Chr4:46346239-46346258 | MsG0480020692.01.T01:three_prime_UTR | 20.0% |
| !! | CATCAATATTAATAATGATG+GGG | + | Chr4:46346528-46346547 | MsG0480020692.01.T01:three_prime_UTR | 20.0% |
| !! | TTTAAATCGTGATAAAGAAA+CGG | + | Chr4:46345997-46346016 | MsG0480020692.01.T01:three_prime_UTR | 20.0% |
| !!! | TCTATATTGAAGTTGAATTT+TGG | + | Chr4:46346307-46346326 | MsG0480020692.01.T01:three_prime_UTR | 20.0% |
| ! | AACCTAATATGAGATTGAAT+AGG | - | Chr4:46345527-46345546 | None:intergenic | 25.0% |
| ! | AATCTCTCTTAACATTTCTA+TGG | - | Chr4:46346784-46346803 | None:intergenic | 25.0% |
| ! | ATCTCTCTTAACATTTCTAT+GGG | - | Chr4:46346783-46346802 | None:intergenic | 25.0% |
| ! | ATTACTAATAATTCAGTCCT+TGG | - | Chr4:46345975-46345994 | None:intergenic | 25.0% |
| ! | CTTCTATATCTTCTCAATTA+AGG | + | Chr4:46345622-46345641 | MsG0480020692.01.T01:CDS | 25.0% |
| ! | TTAATATGTATGGCACAAAA+CGG | + | Chr4:46346249-46346268 | MsG0480020692.01.T01:three_prime_UTR | 25.0% |
| !! | GAAATTAAATTGTGTCACTA+TGG | + | Chr4:46346691-46346710 | MsG0480020692.01.T01:three_prime_UTR | 25.0% |
| !! | GAAGAATTTGAAGTCAAAAA+AGG | - | Chr4:46345606-46345625 | None:intergenic | 25.0% |
| !! | GGAATCATTTTACATTTGTT+AGG | - | Chr4:46346485-46346504 | None:intergenic | 25.0% |
| AACACACTGCTCAAAATATA+TGG | + | Chr4:46346279-46346298 | MsG0480020692.01.T01:three_prime_UTR | 30.0% | |
| CTCCTATTCAATCTCATATT+AGG | + | Chr4:46345522-46345541 | MsG0480020692.01.T01:CDS | 30.0% | |
| GAATATTCAAACAATAGAGC+AGG | + | Chr4:46345426-46345445 | MsG0480020692.01.T01:five_prime_UTR | 30.0% | |
| GATGAAAGTAGTATTAGCAA+TGG | - | Chr4:46346512-46346531 | None:intergenic | 30.0% | |
| GGAGTAAGAATTGAATAAGA+TGG | - | Chr4:46345506-46345525 | None:intergenic | 30.0% | |
| TAGTTCGTAGTTATAAATCG+CGG | + | Chr4:46346173-46346192 | MsG0480020692.01.T01:three_prime_UTR | 30.0% | |
| TCAGAAAGCAATTTATGAAG+AGG | + | Chr4:46346586-46346605 | MsG0480020692.01.T01:three_prime_UTR | 30.0% | |
| ! | AGTAGTATTAGCAATGGTTT+TGG | - | Chr4:46346506-46346525 | None:intergenic | 30.0% |
| ! | GAATCTTTGTTACTTGTGAA+GGG | - | Chr4:46346663-46346682 | None:intergenic | 30.0% |
| ! | GAATTTGAAGTCAAAAAAGG+TGG | - | Chr4:46345603-46345622 | None:intergenic | 30.0% |
| ! | GTATTGATATTGGAGAGAAA+GGG | + | Chr4:46345454-46345473 | MsG0480020692.01.T01:five_prime_UTR | 30.0% |
| ! | TGAATCTTTGTTACTTGTGA+AGG | - | Chr4:46346664-46346683 | None:intergenic | 30.0% |
| ! | TGTATTGATATTGGAGAGAA+AGG | + | Chr4:46345453-46345472 | MsG0480020692.01.T01:five_prime_UTR | 30.0% |
| !! | ATAATCACACAAAGTACTGT+GGG | + | Chr4:46345562-46345581 | MsG0480020692.01.T01:CDS | 30.0% |
| !! | GCAGGATTTTGTATTGATAT+TGG | + | Chr4:46345444-46345463 | MsG0480020692.01.T01:five_prime_UTR | 30.0% |
| !! | TATATTTTGAGCAGTGTGTT+TGG | - | Chr4:46346279-46346298 | None:intergenic | 30.0% |
| !!! | AAGTTGAATTTTGGAATCAG+TGG | + | Chr4:46346316-46346335 | MsG0480020692.01.T01:three_prime_UTR | 30.0% |
| !!! | TGTAATAGTTTTGTGACACT+AGG | + | Chr4:46345814-46345833 | MsG0480020692.01.T01:intron | 30.0% |
| AAGAAACCATACATGTTCAC+AGG | - | Chr4:46346357-46346376 | None:intergenic | 35.0% | |
| AAGTCTAGAAAGCTTATCCA+AGG | + | Chr4:46345955-46345974 | MsG0480020692.01.T01:CDS | 35.0% | |
| AGAATTTATGGATCCAACCT+TGG | - | Chr4:46345870-46345889 | None:intergenic | 35.0% | |
| ATAAGATGGAGTTTCTTGAG+TGG | - | Chr4:46345492-46345511 | None:intergenic | 35.0% | |
| ATTCTCGAAACTTTACACAG+CGG | + | Chr4:46346406-46346425 | MsG0480020692.01.T01:three_prime_UTR | 35.0% | |
| CAAAATTCTGGTGCATGAAT+TGG | - | Chr4:46345663-46345682 | None:intergenic | 35.0% | |
| CAAACTACCAGCAGAATTTA+TGG | - | Chr4:46345882-46345901 | None:intergenic | 35.0% | |
| CACAGTACTTTGTGTGATTA+TGG | - | Chr4:46345563-46345582 | None:intergenic | 35.0% | |
| GGTAGATCTAATGATTGATG+TGG | + | Chr4:46346427-46346446 | MsG0480020692.01.T01:three_prime_UTR | 35.0% | |
| TACTTTGTGTGATTATGGTG+AGG | - | Chr4:46345558-46345577 | None:intergenic | 35.0% | |
| TTTCTGATAGAGTTGTGACT+TGG | - | Chr4:46346573-46346592 | None:intergenic | 35.0% | |
| ! | AAAGGGTGTGAAAATCCTAA+TGG | + | Chr4:46345684-46345703 | MsG0480020692.01.T01:CDS | 35.0% |
| ! | ATGCACCAGAATTTTGCAAA+GGG | + | Chr4:46345667-46345686 | MsG0480020692.01.T01:CDS | 35.0% |
| ! | TGATGATTTTGCAGCAGATA+AGG | + | Chr4:46345838-46345857 | MsG0480020692.01.T01:intron | 35.0% |
| ! | TTTGTTACTTGTGAAGGGAT+TGG | - | Chr4:46346658-46346677 | None:intergenic | 35.0% |
| !! | CATAATCACACAAAGTACTG+TGG | + | Chr4:46345561-46345580 | MsG0480020692.01.T01:CDS | 35.0% |
| AAACCATACATGTTCACAGG+AGG | - | Chr4:46346354-46346373 | None:intergenic | 40.0% | |
| AGTTGTTGAATCGCAACGTT+GGG | + | Chr4:46346118-46346137 | MsG0480020692.01.T01:three_prime_UTR | 40.0% | |
| GTTGGATCCATAAATTCTGC+TGG | + | Chr4:46345872-46345891 | MsG0480020692.01.T01:CDS | 40.0% | |
| TAGTTGTTGAATCGCAACGT+TGG | + | Chr4:46346117-46346136 | MsG0480020692.01.T01:three_prime_UTR | 40.0% | |
| TATCCTCCTGTGAACATGTA+TGG | + | Chr4:46346348-46346367 | MsG0480020692.01.T01:three_prime_UTR | 40.0% | |
| TCACACCCTTTGCAAAATTC+TGG | - | Chr4:46345675-46345694 | None:intergenic | 40.0% | |
| TCTAGACTTAGACATGCATG+AGG | - | Chr4:46345944-46345963 | None:intergenic | 40.0% | |
| ! | AGGCACTTACTGCATTCTTT+TGG | - | Chr4:46345744-46345763 | None:intergenic | 40.0% |
| ! | CATGCACCAGAATTTTGCAA+AGG | + | Chr4:46345666-46345685 | MsG0480020692.01.T01:CDS | 40.0% |
| ! | TGTGACTTGGCATCAGTTTA+TGG | - | Chr4:46346560-46346579 | None:intergenic | 40.0% |
| ACACTTCAAGCCAGCATTAC+AGG | - | Chr4:46345712-46345731 | None:intergenic | 45.0% | |
| AGAAATGCAACCAACGCATC+TGG | - | Chr4:46346214-46346233 | None:intergenic | 45.0% | |
| AGCAGATAAGGTCATCTCCA+AGG | + | Chr4:46345850-46345869 | MsG0480020692.01.T01:intron | 45.0% | |
| CCAGCATTACAGGTTCCATT+AGG | - | Chr4:46345702-46345721 | None:intergenic | 45.0% | |
| CCTAATGGAACCTGTAATGC+TGG | + | Chr4:46345699-46345718 | MsG0480020692.01.T01:CDS | 45.0% | |
| GACTTAGACATGCATGAGGA+AGG | - | Chr4:46345940-46345959 | None:intergenic | 45.0% | |
| GATAAGGTCATCTCCAAGGT+TGG | + | Chr4:46345854-46345873 | MsG0480020692.01.T01:CDS | 45.0% | |
| AACTGCGTTTCCAGATGCGT+TGG | + | Chr4:46346201-46346220 | MsG0480020692.01.T01:three_prime_UTR | 50.0% | |
| CGCATCTGGAAACGCAGTTA+TGG | - | Chr4:46346200-46346219 | None:intergenic | 50.0% | |
| TTGAGAATTCCTAGCCCTCC+AGG | + | Chr4:46346630-46346649 | MsG0480020692.01.T01:three_prime_UTR | 50.0% | |
| CTTGTGAAGGGATTGGAGCC+TGG | - | Chr4:46346651-46346670 | None:intergenic | 55.0% | |
| TGAAGGGATTGGAGCCTGGA+GGG | - | Chr4:46346647-46346666 | None:intergenic | 55.0% | |
| GTGAAGGGATTGGAGCCTGG+AGG | - | Chr4:46346648-46346667 | None:intergenic | 60.0% | |
| GGATTGGAGCCTGGAGGGCT+AGG | - | Chr4:46346642-46346661 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 46345425 | 46346830 | 46345425 | ID=MsG0480020692.01;Name=MsG0480020692.01 |
| Chr4 | mRNA | 46345425 | 46346830 | 46345425 | ID=MsG0480020692.01.T01;Parent=MsG0480020692.01;Name=MsG0480020692.01.T01;_AED=0.48;_eAED=0.48;_QI=52|1|1|1|0|0|2|848|135 |
| Chr4 | exon | 46345425 | 46345753 | 46345425 | ID=MsG0480020692.01.T01:exon:15746;Parent=MsG0480020692.01.T01 |
| Chr4 | exon | 46345852 | 46346830 | 46345852 | ID=MsG0480020692.01.T01:exon:15747;Parent=MsG0480020692.01.T01 |
| Chr4 | five_prime_UTR | 46345425 | 46345476 | 46345425 | ID=MsG0480020692.01.T01:five_prime_utr;Parent=MsG0480020692.01.T01 |
| Chr4 | CDS | 46345477 | 46345753 | 46345477 | ID=MsG0480020692.01.T01:cds;Parent=MsG0480020692.01.T01 |
| Chr4 | CDS | 46345852 | 46345982 | 46345852 | ID=MsG0480020692.01.T01:cds;Parent=MsG0480020692.01.T01 |
| Chr4 | three_prime_UTR | 46345983 | 46346830 | 46345983 | ID=MsG0480020692.01.T01:three_prime_utr;Parent=MsG0480020692.01.T01 |
| Gene Sequence |
| Protein sequence |
