Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020703.01.T01 | AES88520.1 | 97.101 | 69 | 2 | 0 | 1 | 69 | 1 | 69 | 1.98E-41 | 144 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020703.01.T01 | G7JM87 | 97.101 | 69 | 2 | 0 | 1 | 69 | 1 | 69 | 9.46e-42 | 144 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480020703.01 | MsG0480021735.01 | 0.803836 | 2.881760e-49 | 1.463097e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020703.01.T01 | MTR_4g057190 | 97.101 | 69 | 2 | 0 | 1 | 69 | 1 | 69 | 2.40e-45 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020703.01.T01 | AT5G22875 | 78.261 | 69 | 15 | 0 | 1 | 69 | 1 | 69 | 3.75e-36 | 116 |
| MsG0480020703.01.T01 | AT5G22875 | 78.261 | 69 | 15 | 0 | 1 | 69 | 1 | 69 | 3.75e-36 | 116 |
| MsG0480020703.01.T01 | AT5G22875 | 78.261 | 69 | 15 | 0 | 1 | 69 | 1 | 69 | 3.75e-36 | 116 |
Find 14 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAGGACCTTATATTAATAC+TGG | 0.230807 | 4:+46638606 | MsG0480020703.01.T01:CDS |
| TTCTTATAAGTTTCATAATC+AGG | 0.280504 | 4:-46638645 | None:intergenic |
| GGCTTTGTTGGATCAATTAC+CGG | 0.322258 | 4:-46638561 | None:intergenic |
| AATACTGGGTCACGTGATAT+TGG | 0.406093 | 4:+46638621 | MsG0480020703.01.T01:CDS |
| CGTGACCCAGTATTAATATA+AGG | 0.410120 | 4:-46638612 | None:intergenic |
| TGTGGTGAAAATGGCTTTGT+TGG | 0.418803 | 4:-46638573 | None:intergenic |
| CGAAATTTAGTGGTTGCTGC+TGG | 0.423556 | 4:+46638379 | MsG0480020703.01.T01:CDS |
| AATGTAGCTTGTGGTGAAAA+TGG | 0.444463 | 4:-46638582 | None:intergenic |
| GTCACGAAGACGAAATTTAG+TGG | 0.450564 | 4:+46638369 | MsG0480020703.01.T01:CDS |
| TTTGTTTCAAAGTTACGTTC+TGG | 0.451732 | 4:-46638274 | None:intergenic |
| TCACCACAAGCTACATTTCG+AGG | 0.465229 | 4:+46638588 | MsG0480020703.01.T01:CDS |
| TTAACTTACGCCATGTAGAA+AGG | 0.474118 | 4:-46638436 | None:intergenic |
| GAGGACCTTATATTAATACT+GGG | 0.603505 | 4:+46638607 | MsG0480020703.01.T01:CDS |
| GGTCCTCGAAATGTAGCTTG+TGG | 0.650550 | 4:-46638591 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTGATAATTTTCATTTT+TGG | + | Chr4:46638516-46638535 | MsG0480020703.01.T01:intron | 10.0% |
| !!! | TTTTGATAATTTTCATTTTT+GGG | + | Chr4:46638517-46638536 | MsG0480020703.01.T01:intron | 10.0% |
| !! | AACACTCAAAAAATCAATTT+AGG | + | Chr4:46638324-46638343 | MsG0480020703.01.T01:five_prime_UTR | 20.0% |
| !! | ACACTCAAAAAATCAATTTA+GGG | + | Chr4:46638325-46638344 | MsG0480020703.01.T01:five_prime_UTR | 20.0% |
| !! | TTCTTATAAGTTTCATAATC+AGG | - | Chr4:46638648-46638667 | None:intergenic | 20.0% |
| GAGGACCTTATATTAATACT+GGG | + | Chr4:46638607-46638626 | MsG0480020703.01.T01:CDS | 30.0% | |
| TTTGTTTCAAAGTTACGTTC+TGG | - | Chr4:46638277-46638296 | None:intergenic | 30.0% | |
| CGAGGACCTTATATTAATAC+TGG | + | Chr4:46638606-46638625 | MsG0480020703.01.T01:CDS | 35.0% | |
| CGTGACCCAGTATTAATATA+AGG | - | Chr4:46638615-46638634 | None:intergenic | 35.0% | |
| TTAACTTACGCCATGTAGAA+AGG | - | Chr4:46638439-46638458 | None:intergenic | 35.0% | |
| ! | AATGGCTTTTCCTTTCTACA+TGG | + | Chr4:46638426-46638445 | MsG0480020703.01.T01:CDS | 35.0% |
| ! | AATGTAGCTTGTGGTGAAAA+TGG | - | Chr4:46638585-46638604 | None:intergenic | 35.0% |
| !!! | TTTTAGTTCGTCGAAGAAAC+CGG | + | Chr4:46638542-46638561 | MsG0480020703.01.T01:intron | 35.0% |
| AATACTGGGTCACGTGATAT+TGG | + | Chr4:46638621-46638640 | MsG0480020703.01.T01:CDS | 40.0% | |
| GTCACGAAGACGAAATTTAG+TGG | + | Chr4:46638369-46638388 | MsG0480020703.01.T01:CDS | 40.0% | |
| !! | GGCTTTGTTGGATCAATTAC+CGG | - | Chr4:46638564-46638583 | None:intergenic | 40.0% |
| !! | TGTGGTGAAAATGGCTTTGT+TGG | - | Chr4:46638576-46638595 | None:intergenic | 40.0% |
| !! | TTACTTGCTTTTGCTTCAGC+AGG | + | Chr4:46638403-46638422 | MsG0480020703.01.T01:CDS | 40.0% |
| CGAAATTTAGTGGTTGCTGC+TGG | + | Chr4:46638379-46638398 | MsG0480020703.01.T01:CDS | 45.0% | |
| TCACCACAAGCTACATTTCG+AGG | + | Chr4:46638588-46638607 | MsG0480020703.01.T01:CDS | 45.0% | |
| ! | TGCTTTTGCTTCAGCAGGAA+TGG | + | Chr4:46638408-46638427 | MsG0480020703.01.T01:CDS | 45.0% |
| GGTCCTCGAAATGTAGCTTG+TGG | - | Chr4:46638594-46638613 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 46638271 | 46638674 | 46638271 | ID=MsG0480020703.01;Name=MsG0480020703.01 |
| Chr4 | mRNA | 46638271 | 46638674 | 46638271 | ID=MsG0480020703.01.T01;Parent=MsG0480020703.01;Name=MsG0480020703.01.T01;_AED=0.48;_eAED=0.48;_QI=96|1|1|1|1|1|2|0|69 |
| Chr4 | exon | 46638271 | 46638449 | 46638271 | ID=MsG0480020703.01.T01:exon:7237;Parent=MsG0480020703.01.T01 |
| Chr4 | exon | 46638548 | 46638674 | 46638548 | ID=MsG0480020703.01.T01:exon:7238;Parent=MsG0480020703.01.T01 |
| Chr4 | five_prime_UTR | 46638271 | 46638366 | 46638271 | ID=MsG0480020703.01.T01:five_prime_utr;Parent=MsG0480020703.01.T01 |
| Chr4 | CDS | 46638367 | 46638449 | 46638367 | ID=MsG0480020703.01.T01:cds;Parent=MsG0480020703.01.T01 |
| Chr4 | CDS | 46638548 | 46638674 | 46638548 | ID=MsG0480020703.01.T01:cds;Parent=MsG0480020703.01.T01 |
| Gene Sequence |
| Protein sequence |
