AlfalfaGEDB | MsG0480021735.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021735.01

KEGG: K13153
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021735.01.T01	AES89402.1	98.54	137	2	0	1	137	1	137	5.81E-93	275

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021735.01.T01	Q84WS8	64.925	134	44	1	3	133	2	135	1.87E-49	158

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021735.01.T01	A0A072URE6	98.540	137	2	0	1	137	254	390	1.18e-90	278
MsG0480021735.01.T01	G7JNG0	98.540	137	2	0	1	137	1	137	2.77e-93	275

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048152.01	MsG0480021735.01	0.813281	2.771473e-51	1.802135e-48
MsG0080048383.01	MsG0480021735.01	0.841004	6.180353e-58	8.958580e-55
MsG0180000205.01	MsG0480021735.01	0.810736	9.939459e-51	6.039310e-48
MsG0180000568.01	MsG0480021735.01	0.812556	3.994913e-51	2.547990e-48
MsG0180000806.01	MsG0480021735.01	0.843397	1.437125e-58	2.245862e-55
MsG0180000836.01	MsG0480021735.01	0.806285	8.859537e-50	4.790058e-47
MsG0180000982.01	MsG0480021735.01	0.835695	1.444362e-56	1.780064e-53
MsG0180001235.01	MsG0480021735.01	0.802364	5.810952e-49	2.841274e-46
MsG0180001599.01	MsG0480021735.01	0.815910	7.258279e-52	5.068985e-49
MsG0180002547.01	MsG0480021735.01	0.801343	9.417033e-49	4.486472e-46
MsG0180003181.01	MsG0480021735.01	0.805467	1.316203e-49	6.968738e-47
MsG0180003572.01	MsG0480021735.01	0.810734	9.946467e-51	6.043357e-48
MsG0180003922.01	MsG0480021735.01	0.813427	2.573707e-51	1.680253e-48
MsG0180004016.01	MsG0480021735.01	0.832300	1.022807e-55	1.138394e-52
MsG0180004128.01	MsG0480021735.01	0.801013	1.100179e-48	5.198335e-46
MsG0180004861.01	MsG0480021735.01	0.808882	2.488930e-50	1.439719e-47
MsG0180005049.01	MsG0480021735.01	0.800262	1.564811e-48	7.253834e-46
MsG0180005787.01	MsG0480021735.01	0.830866	2.306499e-55	2.460946e-52
MsG0180006046.01	MsG0480021735.01	0.813740	2.196670e-51	1.446315e-48
MsG0180006050.01	MsG0480021735.01	0.802785	4.757431e-49	2.351210e-46
MsG0380011581.01	MsG0480021735.01	0.805072	1.592676e-49	8.347386e-47
MsG0380011614.01	MsG0480021735.01	0.816569	5.168088e-52	3.675198e-49
MsG0380013085.01	MsG0480021735.01	0.800841	1.192919e-48	5.611702e-46
MsG0380013579.01	MsG0480021735.01	0.826330	2.878693e-54	2.691342e-51
MsG0380014175.01	MsG0480021735.01	0.847576	1.059412e-59	1.891417e-56
MsG0380014330.01	MsG0480021735.01	0.800055	1.724505e-48	7.952089e-46
MsG0480018479.01	MsG0480021735.01	0.821459	3.993596e-53	3.250802e-50
MsG0480020703.01	MsG0480021735.01	0.803836	2.881760e-49	1.463097e-46
MsG0480020829.01	MsG0480021735.01	0.808480	3.033703e-50	1.736560e-47
MsG0480021041.01	MsG0480021735.01	0.805323	1.411081e-49	7.443266e-47
MsG0480021706.01	MsG0480021735.01	0.803771	2.973851e-49	1.507255e-46
MsG0480021724.01	MsG0480021735.01	0.834278	3.287403e-56	3.882763e-53
MsG0480021735.01	MsG0480022223.01	0.823987	1.030003e-53	9.007197e-51
MsG0480021735.01	MsG0480022672.01	0.826513	2.603203e-54	2.446512e-51
MsG0480021735.01	MsG0480022780.01	0.801399	9.170318e-49	4.374975e-46
MsG0480021735.01	MsG0480023356.01	0.811668	6.241160e-51	3.886508e-48
MsG0480021735.01	MsG0580024084.01	0.805562	1.257249e-49	6.672537e-47
MsG0480021735.01	MsG0580024339.01	0.828753	7.544772e-55	7.567520e-52
MsG0480021735.01	MsG0580024619.01	0.803889	2.810280e-49	1.428782e-46
MsG0480021735.01	MsG0580024727.01	0.824831	6.523729e-54	5.844553e-51
MsG0480021735.01	MsG0580025756.01	0.834480	2.925990e-56	3.476796e-53
MsG0480021735.01	MsG0580026301.01	0.802856	4.599835e-49	2.277374e-46
MsG0480021735.01	MsG0580026485.01	0.835208	1.918482e-56	2.330018e-53
MsG0480021735.01	MsG0580026718.01	0.819160	1.343605e-52	1.025952e-49
MsG0480021735.01	MsG0580028094.01	0.804397	2.203334e-49	1.134806e-46
MsG0480021735.01	MsG0580028582.01	0.819075	1.405143e-52	1.070445e-49
MsG0480021735.01	MsG0580028597.01	0.838435	2.880981e-57	3.858620e-54
MsG0480021735.01	MsG0580030249.01	0.800351	1.501285e-48	6.975121e-46
MsG0480021735.01	MsG0680031000.01	0.826055	3.346155e-54	3.104087e-51
MsG0480021735.01	MsG0680031654.01	0.819474	1.139659e-52	8.779139e-50
MsG0480021735.01	MsG0680031699.01	0.808366	3.208223e-50	1.831043e-47
MsG0480021735.01	MsG0680031964.01	0.816263	6.053146e-52	4.268851e-49
MsG0480021735.01	MsG0680034445.01	0.816145	6.432232e-52	4.521446e-49
MsG0480021735.01	MsG0680035306.01	0.812336	4.463722e-51	2.830470e-48
MsG0480021735.01	MsG0680035577.01	0.815257	1.014049e-51	6.956492e-49
MsG0480021735.01	MsG0680035765.01	0.808923	2.439319e-50	1.412572e-47
MsG0480021735.01	MsG0680035876.01	0.802190	6.310414e-49	3.071576e-46
MsG0480021735.01	MsG0680035888.01	0.818827	1.599778e-52	1.210421e-49
MsG0480021735.01	MsG0780036186.01	0.813897	2.028536e-51	1.341327e-48
MsG0480021735.01	MsG0780036544.01	-0.802101	6.580743e-49	3.195997e-46
MsG0480021735.01	MsG0780039587.01	0.814921	1.204401e-51	8.186984e-49
MsG0480021735.01	MsG0780040569.01	0.821651	3.605758e-53	2.951048e-50
MsG0480021735.01	MsG0780040845.01	0.813449	2.544751e-51	1.662461e-48
MsG0480021735.01	MsG0780041268.01	0.842058	3.260751e-58	4.884879e-55
MsG0480021735.01	MsG0780041482.01	0.806385	8.440104e-50	4.574742e-47
MsG0480021735.01	MsG0880042033.01	0.843322	1.504581e-58	2.345741e-55
MsG0480021735.01	MsG0880042206.01	0.814500	1.492719e-51	1.003344e-48
MsG0480021735.01	MsG0880042399.01	-0.818156	2.270886e-52	1.686643e-49
MsG0480021735.01	MsG0880042760.01	0.806510	7.942090e-50	4.318713e-47
MsG0480021735.01	MsG0880043162.01	0.802889	4.527067e-49	2.243268e-46
MsG0480021735.01	MsG0880044975.01	0.845152	4.852984e-59	8.012824e-56
MsG0480021735.01	MsG0880045656.01	0.848055	7.819079e-60	1.417133e-56
MsG0480021735.01	MsG0880045684.01	0.829654	4.561843e-55	4.698632e-52
MsG0480021735.01	MsG0880045951.01	0.826025	3.402095e-54	3.153239e-51
MsG0480021735.01	MsG0880046517.01	0.809860	1.535824e-50	9.118427e-48
MsG0480021735.01	MsG0880047212.01	0.807868	4.096017e-50	2.307018e-47
MsG0380015607.01	MsG0480021735.01	0.806538	7.835426e-50	4.263765e-47
MsG0380016005.01	MsG0480021735.01	0.811126	8.182596e-51	5.023524e-48
MsG0380016513.01	MsG0480021735.01	0.813827	2.101537e-51	1.386938e-48
MsG0380016604.01	MsG0480021735.01	0.817097	3.935093e-52	2.839022e-49
MsG0380017476.01	MsG0480021735.01	0.810428	1.158552e-50	6.981479e-48
MsG0380017797.01	MsG0480021735.01	0.819750	9.859507e-53	7.653575e-50
MsG0380018033.01	MsG0480021735.01	0.843790	1.128223e-58	1.784756e-55
MsG0480018235.01	MsG0480021735.01	0.850009	2.239824e-60	4.326340e-57
MsG0280006545.01	MsG0480021735.01	0.830872	2.299578e-55	2.453939e-52
MsG0280006679.01	MsG0480021735.01	0.801905	7.221033e-49	3.489587e-46
MsG0280006892.01	MsG0480021735.01	0.801683	8.019756e-49	3.853592e-46
MsG0280007161.01	MsG0480021735.01	0.814019	1.906643e-51	1.264908e-48
MsG0280007847.01	MsG0480021735.01	0.852087	5.809895e-61	1.200554e-57
MsG0280007992.01	MsG0480021735.01	0.806926	6.488320e-50	3.566092e-47
MsG0280009054.01	MsG0480021735.01	0.804091	2.550821e-49	1.303573e-46
MsG0280009575.01	MsG0480021735.01	0.821192	4.601099e-53	3.717956e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021735.01.T01	MTR_4g074450	98.540	137	2	0	1	137	1	137	7.03e-97	275
MsG0480021735.01.T01	MTR_4g108000	98.540	137	2	0	1	137	254	390	2.99e-94	278
MsG0480021735.01.T01	MTR_4g108000	98.540	137	2	0	1	137	269	405	4.93e-94	277
MsG0480021735.01.T01	MTR_4g074450	97.727	132	3	0	1	132	1	132	2.35e-93	267
MsG0480021735.01.T01	MTR_4g108000	93.571	140	7	1	1	138	269	408	2.73e-90	268
MsG0480021735.01.T01	MTR_1g012600	44.048	84	45	1	49	132	47	128	7.57e-18	77.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021735.01.T01	AT3G07860	64.925	134	44	1	3	133	2	135	1.90e-50	158
MsG0480021735.01.T01	AT3G07860	64.925	134	44	1	3	133	2	135	1.90e-50	158
MsG0480021735.01.T01	AT3G07860	65.414	133	43	1	3	132	2	134	2.58e-50	157
MsG0480021735.01.T01	AT5G25340	40.964	83	47	1	50	132	19	99	1.64e-17	75.5
MsG0480021735.01.T01	AT4G32270	33.333	84	54	1	49	132	37	118	2.59e-15	70.5

Find 28 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGATGACGTGCTTCAAAATT+TGG	0.116379	4:+63692990	MsG0480021735.01.T01:CDS
ATCTTCAAGCGTTGGATTCT+TGG	0.226667	4:-63692614	None:intergenic
AACTCTCATTGGACTTGAAT+TGG	0.259182	4:+63692642	MsG0480021735.01.T01:CDS
TTACATGGAACAATCTAGTA+TGG	0.294231	4:+63692777	MsG0480021735.01.T01:CDS
GAAGGTGTTGAAAGCATTAT+TGG	0.319812	4:+63692567	MsG0480021735.01.T01:CDS
TGGGGCATCGTCACATTTCT+TGG	0.338798	4:+63692797	MsG0480021735.01.T01:CDS
TTAATTACAGGAGGAGTGTT+TGG	0.352815	4:+63692923	MsG0480021735.01.T01:intron
TGATGTGATTCTTATGAATA+CGG	0.365027	4:+63692711	MsG0480021735.01.T01:CDS
TCTTCAAGCGTTGGATTCTT+GGG	0.407150	4:-63692613	None:intergenic
TAATTACAGGAGGAGTGTTT+GGG	0.410370	4:+63692924	MsG0480021735.01.T01:intron
GAAGCACGTCATCATCGTTA+AGG	0.413624	4:-63692981	None:intergenic
GATGACGTGCTTCAAAATTT+GGG	0.417532	4:+63692991	MsG0480021735.01.T01:CDS
ACTCTCATTGGACTTGAATT+GGG	0.418691	4:+63692643	MsG0480021735.01.T01:CDS
TTGGGTACATCTGCAAGTAT+TGG	0.422385	4:-63692595	None:intergenic
GGGCGTTCGAAATAATTCTC+AGG	0.483534	4:+63693011	MsG0480021735.01.T01:CDS
ATACAAGAGCGGTTTGAAGA+AGG	0.490103	4:+63692543	MsG0480021735.01.T01:CDS
GTTTCCACATCTTCAAGCGT+TGG	0.516833	4:-63692622	None:intergenic
GAAGATGTGGAAACTCTCAT+TGG	0.517568	4:+63692631	MsG0480021735.01.T01:CDS
GAATCCAACGCTTGAAGATG+TGG	0.524645	4:+63692618	MsG0480021735.01.T01:CDS
CAAGAAGAAAGTTAATTACA+TGG	0.532345	4:+63692762	MsG0480021735.01.T01:CDS
TATGAATACGGCAACTCTCA+AGG	0.534810	4:+63692723	MsG0480021735.01.T01:CDS
TACATGGAACAATCTAGTAT+GGG	0.546371	4:+63692778	MsG0480021735.01.T01:CDS
AGATGAGATGGGAGAAGGTG+CGG	0.572900	4:+63692519	None:intergenic
GGTGTTGAAAGCATTATTGG+AGG	0.583098	4:+63692570	MsG0480021735.01.T01:CDS
CGATGAGATGAGATGGGAGA+AGG	0.633936	4:+63692514	None:intergenic
ACATGGAACAATCTAGTATG+GGG	0.649258	4:+63692779	MsG0480021735.01.T01:CDS
GAGCGGTTTGAAGAAGGCGA+AGG	0.652966	4:+63692549	MsG0480021735.01.T01:CDS
GAAGGTGCGGAATACAAGAG+CGG	0.727904	4:+63692532	MsG0480021735.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATCTGATTGATTTTAATTAC+AGG	+	Chr4:63692911-63692930	MsG0480021735.01.T01:intron	20.0%
!	CAAGAAGAAAGTTAATTACA+TGG	+	Chr4:63692762-63692781	MsG0480021735.01.T01:CDS	25.0%
!	TGATGTGATTCTTATGAATA+CGG	+	Chr4:63692711-63692730	MsG0480021735.01.T01:CDS	25.0%
!!!	TGATTGATTTTAATTACAGG+AGG	+	Chr4:63692914-63692933	MsG0480021735.01.T01:intron	25.0%
	TACATGGAACAATCTAGTAT+GGG	+	Chr4:63692778-63692797	MsG0480021735.01.T01:CDS	30.0%
	TTACATGGAACAATCTAGTA+TGG	+	Chr4:63692777-63692796	MsG0480021735.01.T01:CDS	30.0%
!	TTCAATGTGTTGCTAATCAA+TGG	-	Chr4:63692858-63692877	None:intergenic	30.0%
!!!	GCGAATTTCTGTTTTGAAAT+TGG	+	Chr4:63692675-63692694	MsG0480021735.01.T01:CDS	30.0%
	AACTCTCATTGGACTTGAAT+TGG	+	Chr4:63692642-63692661	MsG0480021735.01.T01:CDS	35.0%
	ACATGGAACAATCTAGTATG+GGG	+	Chr4:63692779-63692798	MsG0480021735.01.T01:CDS	35.0%
	ACTCTCATTGGACTTGAATT+GGG	+	Chr4:63692643-63692662	MsG0480021735.01.T01:CDS	35.0%
	GATGACGTGCTTCAAAATTT+GGG	+	Chr4:63692991-63693010	MsG0480021735.01.T01:CDS	35.0%
!	TGATGACGTGCTTCAAAATT+TGG	+	Chr4:63692990-63693009	MsG0480021735.01.T01:CDS	35.0%
!!	GAAGGTGTTGAAAGCATTAT+TGG	+	Chr4:63692567-63692586	MsG0480021735.01.T01:CDS	35.0%
!!	TAATTACAGGAGGAGTGTTT+GGG	+	Chr4:63692924-63692943	MsG0480021735.01.T01:intron	35.0%
!!	TTAATTACAGGAGGAGTGTT+TGG	+	Chr4:63692923-63692942	MsG0480021735.01.T01:intron	35.0%
	ATACAAGAGCGGTTTGAAGA+AGG	+	Chr4:63692543-63692562	MsG0480021735.01.T01:CDS	40.0%
	GAAGATGTGGAAACTCTCAT+TGG	+	Chr4:63692631-63692650	MsG0480021735.01.T01:CDS	40.0%
	TATGAATACGGCAACTCTCA+AGG	+	Chr4:63692723-63692742	MsG0480021735.01.T01:CDS	40.0%
	TTGGGTACATCTGCAAGTAT+TGG	-	Chr4:63692598-63692617	None:intergenic	40.0%
!!	ATCTTCAAGCGTTGGATTCT+TGG	-	Chr4:63692617-63692636	None:intergenic	40.0%
!!	GGTGTTGAAAGCATTATTGG+AGG	+	Chr4:63692570-63692589	MsG0480021735.01.T01:CDS	40.0%
!!	TCTTCAAGCGTTGGATTCTT+GGG	-	Chr4:63692616-63692635	None:intergenic	40.0%
	GAAGCACGTCATCATCGTTA+AGG	-	Chr4:63692984-63693003	None:intergenic	45.0%
	GAATCCAACGCTTGAAGATG+TGG	+	Chr4:63692618-63692637	MsG0480021735.01.T01:CDS	45.0%
	GGGCGTTCGAAATAATTCTC+AGG	+	Chr4:63693011-63693030	MsG0480021735.01.T01:CDS	45.0%
	GTTTCCACATCTTCAAGCGT+TGG	-	Chr4:63692625-63692644	None:intergenic	45.0%
!	GAAGGTGCGGAATACAAGAG+CGG	+	Chr4:63692532-63692551	MsG0480021735.01.T01:CDS	50.0%
!!	TGGGGCATCGTCACATTTCT+TGG	+	Chr4:63692797-63692816	MsG0480021735.01.T01:CDS	50.0%
	GAGCGGTTTGAAGAAGGCGA+AGG	+	Chr4:63692549-63692568	MsG0480021735.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	63692526	63693056	63692526	ID=MsG0480021735.01;Name=MsG0480021735.01
Chr4	mRNA	63692526	63693056	63692526	ID=MsG0480021735.01.T01;Parent=MsG0480021735.01;Name=MsG0480021735.01.T01;_AED=0.50;_eAED=0.52;_QI=0\|0\|0\|1\|1\|1\|2\|0\|138
Chr4	exon	63692526	63692818	63692526	ID=MsG0480021735.01.T01:exon:8452;Parent=MsG0480021735.01.T01
Chr4	exon	63692933	63693056	63692933	ID=MsG0480021735.01.T01:exon:8451;Parent=MsG0480021735.01.T01
Chr4	CDS	63692526	63692818	63692526	ID=MsG0480021735.01.T01:cds;Parent=MsG0480021735.01.T01
Chr4	CDS	63692933	63693056	63692933	ID=MsG0480021735.01.T01:cds;Parent=MsG0480021735.01.T01

Gene Sequence

>MsG0480021735.01.T01

ATGGGAGAAGGTGCGGAATACAAGAGCGGTTTGAAGAAGGCGAAGGTGTTGAAAGCATTATTGGAGGATCCAATACTTGCAGATGTACCCAAGAATCCAACGCTTGAAGATGTGGAAACTCTCATTGGACTTGAATTGGGTAGTGCAATGCGAATTTCTGTTTTGAAATTGGATGCAACTTCTTTTGATGTGATTCTTATGAATACGGCAACTCTCAAGGATTTGAAACTTGCTATCAAGAAGAAAGTTAATTACATGGAACAATCTAGTATGGGGCATCGTCACATTTCTTGGAGGAGTGTTTGGGCGAATTATTGTTTGTCATTCGATAACAACAAGCTCCTTAACGATGATGACGTGCTTCAAAATTTGGGCGTTCGAAATAATTCTCAGGTTTCTTCTACTTATCTATTCTAA

Protein sequence

>MsG0480021735.01.T01

MGEGAEYKSGLKKAKVLKALLEDPILADVPKNPTLEDVETLIGLELGSAMRISVLKLDATSFDVILMNTATLKDLKLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDDVLQNLGVRNNSQVSSTYLF*