AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020714.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020714.01.T01 MTR_4g057470 93.720 207 6 3 1 206 1 201 2.16e-129 365
MsG0480020714.01.T01 MTR_2g084270 63.596 228 54 7 1 226 1 201 7.40e-94 275
MsG0480020714.01.T01 MTR_1g090663 43.455 191 78 4 10 199 8 169 6.44e-43 144
MsG0480020714.01.T01 MTR_4g063070 36.652 221 70 10 23 204 16 205 1.77e-20 89.0
MsG0480020714.01.T01 MTR_2g096360 31.651 218 103 10 13 204 7 204 7.64e-20 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020714.01.T01 AT5G22930 56.481 216 76 4 1 207 1 207 3.84e-75 227
MsG0480020714.01.T01 AT3G44940 57.078 219 73 5 5 207 3 216 7.05e-62 194
MsG0480020714.01.T01 AT2G28140 47.573 206 76 3 1 204 1 176 6.77e-53 170
MsG0480020714.01.T01 AT2G28690 35.938 192 116 3 12 203 8 192 1.48e-30 113
MsG0480020714.01.T01 AT5G59760 32.663 199 120 5 9 204 3 190 1.89e-20 86.7

Find 83 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGTAATGTTTGGGGTTA+AGG 0.173088 4:-46834384 MsG0480020714.01.T01:three_prime_UTR
ACCGTCTGATCTTAAATTAA+TGG 0.229367 4:-46834588 MsG0480020714.01.T01:three_prime_UTR
AGAGTTGTGGGAGAGAAATA+AGG 0.246169 4:+46835739 None:intergenic
TTGGTAATGTTTGGGGTTAA+GGG 0.279921 4:-46834383 MsG0480020714.01.T01:three_prime_UTR
GATAAACCTTTGCCAGAAAA+AGG 0.289825 4:-46834974 MsG0480020714.01.T01:CDS
AATGAGAAGAAAAGAAGAAA+AGG 0.337367 4:+46835770 None:intergenic
AATGTAGTAGAGATCTTTAT+AGG 0.369590 4:-46834137 MsG0480020714.01.T01:three_prime_UTR
AACGTACCCCTCTTCTATCT+TGG 0.371913 4:-46835677 MsG0480020714.01.T01:CDS
ATGAGAAGAAAAGAAGAAAA+GGG 0.384334 4:+46835771 None:intergenic
GTTCTGTTCTTGTTTGTTGT+GGG 0.391019 4:+46835029 None:intergenic
TGGGAGAGAGACAGAGTTGT+GGG 0.399773 4:+46835727 None:intergenic
GAGTTGTGGGAGAGAAATAA+GGG 0.403235 4:+46835740 None:intergenic
GGAAGATTCAATAGTAGAAA+AGG 0.405440 4:-46834751 MsG0480020714.01.T01:three_prime_UTR
TGATAGTGATATTCCATTGT+TGG 0.406170 4:+46835078 None:intergenic
ATTGAATCAATCATCATCAT+TGG 0.412861 4:+46835004 None:intergenic
TGCTGATGCTGTGGTGGTTG+AGG 0.414937 4:+46834815 None:intergenic
AGATTCCAGACAAAGGATCT+TGG 0.415576 4:+46835161 None:intergenic
TGTTTCTTTGTGAAAGGAAA+AGG 0.430527 4:-46834323 MsG0480020714.01.T01:three_prime_UTR
GAAGATTCAATAGTAGAAAA+GGG 0.440336 4:-46834750 MsG0480020714.01.T01:three_prime_UTR
CTCCATTGTTGTGTACATCA+AGG 0.444003 4:+46835357 None:intergenic
ACGTACCCCTCTTCTATCTT+GGG 0.445573 4:-46835676 MsG0480020714.01.T01:CDS
AAGTGATTTGAGTGTGTGTA+TGG 0.450795 4:-46834254 MsG0480020714.01.T01:three_prime_UTR
GGGTGTCTCCATTGAGGTAA+TGG 0.451885 4:+46834890 None:intergenic
TCCCACTCTTTCTAAGATCA+TGG 0.456841 4:-46835709 MsG0480020714.01.T01:exon
GTAATGGTCCTGCAAGCAAA+AGG 0.461418 4:+46834906 None:intergenic
CAAAACCAAGATCCTTTGTC+TGG 0.462496 4:-46835166 MsG0480020714.01.T01:CDS
TGTTCTGTTCTTGTTTGTTG+TGG 0.463846 4:+46835028 None:intergenic
GAAGTATAGGTACCTTTCCT+TGG 0.469839 4:+46835643 None:intergenic
AATAGTAACAGTGCTAGGTT+AGG 0.477209 4:+46834659 None:intergenic
AAGGACTCCAATGGTGGTGG+AGG 0.485852 4:+46834869 None:intergenic
AAACAAAACTAGAATGTGAA+TGG 0.487451 4:+46834341 None:intergenic
CTGATCTAGCATATGTATAG+TGG 0.495805 4:-46834448 MsG0480020714.01.T01:three_prime_UTR
GTCAATGGAGTACAATCCTC+TGG 0.508694 4:+46834684 None:intergenic
GTGGGAGAGAGACAGAGTTG+TGG 0.509113 4:+46835726 None:intergenic
GTCCATGATCTTAGAAAGAG+TGG 0.515664 4:+46835707 None:intergenic
TGGTTGAGGTGGTGAATGAA+TGG 0.519132 4:+46834829 None:intergenic
TAATGATTAGCATTAGCAAA+TGG 0.521168 4:-46834478 MsG0480020714.01.T01:three_prime_UTR
GTGCTAGGTTAGGTAGTCAA+TGG 0.523482 4:+46834669 None:intergenic
ACCATTAATTTAAGATCAGA+CGG 0.523566 4:+46834587 None:intergenic
ATCAATAGAACTAGTTCTAT+CGG 0.527449 4:+46834185 None:intergenic
TGAGAAGAAAAGAAGAAAAG+GGG 0.527624 4:+46835772 None:intergenic
GAGACACCCTCCACCACCAT+TGG 0.528054 4:-46834876 MsG0480020714.01.T01:CDS
TTGCAGGACCATTACCTCAA+TGG 0.529726 4:-46834898 MsG0480020714.01.T01:CDS
TTACAAGCAGTGATGAAAGC+TGG 0.538923 4:-46834944 MsG0480020714.01.T01:CDS
AGCAAAAGGGTCTGAAGCAA+AGG 0.539159 4:+46834920 None:intergenic
TTCTGTTCTTGTTTGTTGTG+GGG 0.540016 4:+46835030 None:intergenic
TAGCATATGTATAGTGGTGG+AGG 0.543822 4:-46834442 MsG0480020714.01.T01:three_prime_UTR
ACTAGTTCTATTGATCGTAA+AGG 0.544788 4:-46834177 MsG0480020714.01.T01:three_prime_UTR
TCAAAGGACTCCAATGGTGG+TGG 0.550124 4:+46834866 None:intergenic
GGAATTGAATCTTCCAACAA+TGG 0.550626 4:-46835091 MsG0480020714.01.T01:CDS
AGTAAGCCCAAGATAGAAGA+GGG 0.552955 4:+46835670 None:intergenic
TGTTGCTGCTGATGCTGTGG+TGG 0.555209 4:+46834809 None:intergenic
AAGTCAATAGTAACAGTGCT+AGG 0.556813 4:+46834654 None:intergenic
TGTTCTTGTTTGTTGTGGGG+AGG 0.562798 4:+46835033 None:intergenic
TGATGCTGTGGTGGTTGAGG+TGG 0.562813 4:+46834818 None:intergenic
TTCATTCATCACAATACTAG+AGG 0.563788 4:-46834633 MsG0480020714.01.T01:three_prime_UTR
CTTCCACAGTTATGTTAACA+AGG 0.567713 4:+46834769 None:intergenic
AGATTCTTATTATTGTTGCA+CGG 0.569330 4:-46834226 MsG0480020714.01.T01:three_prime_UTR
TAATGGTCCTGCAAGCAAAA+GGG 0.574855 4:+46834907 None:intergenic
ATAGAACTAGTTCTATCGGT+TGG 0.577825 4:+46834189 None:intergenic
ATCTAGCATATGTATAGTGG+TGG 0.580763 4:-46834445 MsG0480020714.01.T01:three_prime_UTR
AGTTGTTGCTGCTGATGCTG+TGG 0.582168 4:+46834806 None:intergenic
GGTGGAGGGTGTCTCCATTG+AGG 0.584272 4:+46834884 None:intergenic
TGGTACTTATTCTCAGCAGA+AGG 0.589538 4:+46834704 None:intergenic
AGTGATTTGAGTGTGTGTAT+GGG 0.603082 4:-46834253 MsG0480020714.01.T01:three_prime_UTR
ATGCTTGAGATTCCAGACAA+AGG 0.616707 4:+46835154 None:intergenic
TCTTGGGCTTACTACTGCCA+AGG 0.616963 4:-46835660 MsG0480020714.01.T01:CDS
TAGTAAGCCCAAGATAGAAG+AGG 0.618226 4:+46835669 None:intergenic
GGCTTACTACTGCCAAGGAA+AGG 0.619012 4:-46835655 MsG0480020714.01.T01:intron
AACCTTGATGTACACAACAA+TGG 0.621287 4:-46835359 MsG0480020714.01.T01:CDS
AGGACTCCAATGGTGGTGGA+GGG 0.621423 4:+46834870 None:intergenic
GGAATCTCAAAGGACTCCAA+TGG 0.624448 4:+46834860 None:intergenic
ATCTCAAAGGACTCCAATGG+TGG 0.631703 4:+46834863 None:intergenic
CTGCTGAGAATAAGTACCAG+AGG 0.635351 4:-46834700 MsG0480020714.01.T01:three_prime_UTR
GTAAACTAATTAATGTAGTG+CGG 0.642545 4:-46834278 MsG0480020714.01.T01:three_prime_UTR
GGTCACAGGTGGAATCTCAA+AGG 0.642891 4:+46834850 None:intergenic
CAAAACTAGAATGTGAATGG+AGG 0.644567 4:+46834344 None:intergenic
GGTGGTGAATGAATGGTCAC+AGG 0.654394 4:+46834836 None:intergenic
TCCATGATCTTAGAAAGAGT+GGG 0.664683 4:+46835708 None:intergenic
AGTCCTTGTTAACATAACTG+TGG 0.672508 4:-46834772 MsG0480020714.01.T01:exon
GGTGAATGAATGGTCACAGG+TGG 0.686774 4:+46834839 None:intergenic
GTAAGCCCAAGATAGAAGAG+GGG 0.693101 4:+46835671 None:intergenic
AATTGGATCTAGTTGCACGT+CGG 0.707549 4:+46834553 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAATTTATTAAAAAAATG+GGG + Chr4:46834490-46834509 None:intergenic 10.0%
!!! TTTTTTGTAATATAATTGTA+AGG - Chr4:46835609-46835628 MsG0480020714.01.T01:intron 10.0%
!! TATCAATTAAAAAACTAAGT+TGG + Chr4:46835385-46835404 None:intergenic 15.0%
!!! AAGAAAAAATTCTTGATTTT+GGG - Chr4:46835500-46835519 MsG0480020714.01.T01:intron 15.0%
!!! AAAAACTAAGTTGGTAAAAT+TGG + Chr4:46835376-46835395 None:intergenic 20.0%
!!! AGTTAGTCTTATAAACATTT+TGG + Chr4:46834350-46834369 None:intergenic 20.0%
!!! GAAGAAAAAATTCTTGATTT+TGG - Chr4:46835499-46835518 MsG0480020714.01.T01:intron 20.0%
!!! GTTAGTCTCATAAAATTAAA+AGG + Chr4:46834388-46834407 None:intergenic 20.0%
! AAACAAAACTAGAATGTGAA+TGG + Chr4:46835571-46835590 None:intergenic 25.0%
! AATGAGAAGAAAAGAAGAAA+AGG + Chr4:46834142-46834161 None:intergenic 25.0%
! AATGTAGTAGAGATCTTTAT+AGG - Chr4:46835772-46835791 MsG0480020714.01.T01:five_prime_UTR 25.0%
! AGATTCTTATTATTGTTGCA+CGG - Chr4:46835683-46835702 MsG0480020714.01.T01:CDS 25.0%
! ATGAGAAGAAAAGAAGAAAA+GGG + Chr4:46834141-46834160 None:intergenic 25.0%
! ATTCTTCATAGTTACATTTG+AGG - Chr4:46834311-46834330 MsG0480020714.01.T01:three_prime_UTR 25.0%
! ATTGAATCAATCATCATCAT+TGG + Chr4:46834908-46834927 None:intergenic 25.0%
! GAAGATTCAATAGTAGAAAA+GGG - Chr4:46835159-46835178 MsG0480020714.01.T01:CDS 25.0%
! GTAAACTAATTAATGTAGTG+CGG - Chr4:46835631-46835650 MsG0480020714.01.T01:intron 25.0%
! TAATGATTAGCATTAGCAAA+TGG - Chr4:46835431-46835450 MsG0480020714.01.T01:intron 25.0%
!! AAACATTTTGGATGAATAGT+GGG + Chr4:46834338-46834357 None:intergenic 25.0%
!! ACCATTAATTTAAGATCAGA+CGG + Chr4:46835325-46835344 None:intergenic 25.0%
!! CACTAATGATCATTTTACAA+CGG + Chr4:46834431-46834450 None:intergenic 25.0%
!! CTTTGTTGAAAAGTTCTTTT+AGG + Chr4:46834640-46834659 None:intergenic 25.0%
!! TAAACATTTTGGATGAATAG+TGG + Chr4:46834339-46834358 None:intergenic 25.0%
!! TAACGAAAGAAAAGAAGTAT+AGG + Chr4:46834282-46834301 None:intergenic 25.0%
!! TGTAAAAGTTTACCTTTTTC+TGG + Chr4:46834950-46834969 None:intergenic 25.0%
!!! AATTCTTGATTTTGGGTTTT+TGG - Chr4:46835507-46835526 MsG0480020714.01.T01:intron 25.0%
!!! ACTTTTCTAAGAAAAGTCTT+TGG + Chr4:46834697-46834716 None:intergenic 25.0%
!!! ATCAATAGAACTAGTTCTAT+CGG + Chr4:46835727-46835746 None:intergenic 25.0%
!!! TAGTTTTGTTTCTTTGTGAA+AGG - Chr4:46835580-46835599 MsG0480020714.01.T01:intron 25.0%
!!! TTTGTTGTTGTTTTTGTTGT+TGG + Chr4:46834727-46834746 None:intergenic 25.0%
ACCGTCTGATCTTAAATTAA+TGG - Chr4:46835321-46835340 MsG0480020714.01.T01:CDS 30.0%
GGAAGATTCAATAGTAGAAA+AGG - Chr4:46835158-46835177 MsG0480020714.01.T01:CDS 30.0%
TGAGAAGAAAAGAAGAAAAG+GGG + Chr4:46834140-46834159 None:intergenic 30.0%
TGTTTCTTTGTGAAAGGAAA+AGG - Chr4:46835586-46835605 MsG0480020714.01.T01:intron 30.0%
TTCATTCATCACAATACTAG+AGG - Chr4:46835276-46835295 MsG0480020714.01.T01:CDS 30.0%
! GATTTTTGTGATACAGTCAA+TGG - Chr4:46834514-46834533 MsG0480020714.01.T01:three_prime_UTR 30.0%
! TGATAGTGATATTCCATTGT+TGG + Chr4:46834834-46834853 None:intergenic 30.0%
!! ACTAGTTCTATTGATCGTAA+AGG - Chr4:46835732-46835751 MsG0480020714.01.T01:five_prime_UTR 30.0%
!!! TAGAAAAGGGTTTTTTGTGA+AGG - Chr4:46835172-46835191 MsG0480020714.01.T01:CDS 30.0%
!!! TTGGGTTTTTGGTAATGTTT+GGG - Chr4:46835518-46835537 MsG0480020714.01.T01:intron 30.0%
!!! TTTGGGTTTTTGGTAATGTT+TGG - Chr4:46835517-46835536 MsG0480020714.01.T01:intron 30.0%
AACCTTGATGTACACAACAA+TGG - Chr4:46834550-46834569 MsG0480020714.01.T01:three_prime_UTR 35.0%
AAGTCAATAGTAACAGTGCT+AGG + Chr4:46835258-46835277 None:intergenic 35.0%
AAGTGATTTGAGTGTGTGTA+TGG - Chr4:46835655-46835674 MsG0480020714.01.T01:intron 35.0%
AATAGTAACAGTGCTAGGTT+AGG + Chr4:46835253-46835272 None:intergenic 35.0%
AGTCCTTGTTAACATAACTG+TGG - Chr4:46835137-46835156 MsG0480020714.01.T01:CDS 35.0%
AGTGATTTGAGTGTGTGTAT+GGG - Chr4:46835656-46835675 MsG0480020714.01.T01:CDS 35.0%
ATCTAGCATATGTATAGTGG+TGG - Chr4:46835464-46835483 MsG0480020714.01.T01:intron 35.0%
CAAAACTAGAATGTGAATGG+AGG + Chr4:46835568-46835587 None:intergenic 35.0%
CAACAACAACAACAAAAGAG+AGG - Chr4:46834797-46834816 MsG0480020714.01.T01:CDS 35.0%
CTGATCTAGCATATGTATAG+TGG - Chr4:46835461-46835480 MsG0480020714.01.T01:intron 35.0%
CTTCCACAGTTATGTTAACA+AGG + Chr4:46835143-46835162 None:intergenic 35.0%
GATAAACCTTTGCCAGAAAA+AGG - Chr4:46834935-46834954 MsG0480020714.01.T01:CDS 35.0%
GGAATTGAATCTTCCAACAA+TGG - Chr4:46834818-46834837 MsG0480020714.01.T01:CDS 35.0%
TCCATGATCTTAGAAAGAGT+GGG + Chr4:46834204-46834223 None:intergenic 35.0%
! TGTTCTGTTCTTGTTTGTTG+TGG + Chr4:46834884-46834903 None:intergenic 35.0%
!! AGTTTACCTTTTTCTGGCAA+AGG + Chr4:46834944-46834963 None:intergenic 35.0%
!! ATAGAACTAGTTCTATCGGT+TGG + Chr4:46835723-46835742 None:intergenic 35.0%
!! GTTCTGTTCTTGTTTGTTGT+GGG + Chr4:46834883-46834902 None:intergenic 35.0%
!! TTCTGTTCTTGTTTGTTGTG+GGG + Chr4:46834882-46834901 None:intergenic 35.0%
!! TTGGTAATGTTTGGGGTTAA+GGG - Chr4:46835526-46835545 MsG0480020714.01.T01:intron 35.0%
!! TTTGGTAATGTTTGGGGTTA+AGG - Chr4:46835525-46835544 MsG0480020714.01.T01:intron 35.0%
!!! TGGGTTTTTGGTAATGTTTG+GGG - Chr4:46835519-46835538 MsG0480020714.01.T01:intron 35.0%
AATTGGATCTAGTTGCACGT+CGG + Chr4:46835359-46835378 None:intergenic 40.0%
AGAGTTGTGGGAGAGAAATA+AGG + Chr4:46834173-46834192 None:intergenic 40.0%
AGATTCCAGACAAAGGATCT+TGG + Chr4:46834751-46834770 None:intergenic 40.0%
AGTAAGCCCAAGATAGAAGA+GGG + Chr4:46834242-46834261 None:intergenic 40.0%
ATGCTTGAGATTCCAGACAA+AGG + Chr4:46834758-46834777 None:intergenic 40.0%
CAAAACCAAGATCCTTTGTC+TGG - Chr4:46834743-46834762 MsG0480020714.01.T01:three_prime_UTR 40.0%
CTCCATTGTTGTGTACATCA+AGG + Chr4:46834555-46834574 None:intergenic 40.0%
GAAGTATAGGTACCTTTCCT+TGG + Chr4:46834269-46834288 None:intergenic 40.0%
GAGTTGTGGGAGAGAAATAA+GGG + Chr4:46834172-46834191 None:intergenic 40.0%
GTCCATGATCTTAGAAAGAG+TGG + Chr4:46834205-46834224 None:intergenic 40.0%
TAATGGTCCTGCAAGCAAAA+GGG + Chr4:46835005-46835024 None:intergenic 40.0%
TAGCATATGTATAGTGGTGG+AGG - Chr4:46835467-46835486 MsG0480020714.01.T01:intron 40.0%
TAGTAAGCCCAAGATAGAAG+AGG + Chr4:46834243-46834262 None:intergenic 40.0%
TGGTACTTATTCTCAGCAGA+AGG + Chr4:46835208-46835227 None:intergenic 40.0%
TTACAAGCAGTGATGAAAGC+TGG - Chr4:46834965-46834984 MsG0480020714.01.T01:CDS 40.0%
TTTACAACGGTTCTTCTGAC+AGG + Chr4:46834418-46834437 None:intergenic 40.0%
!! TCCCACTCTTTCTAAGATCA+TGG - Chr4:46834200-46834219 MsG0480020714.01.T01:three_prime_UTR 40.0%
AACGTACCCCTCTTCTATCT+TGG - Chr4:46834232-46834251 MsG0480020714.01.T01:three_prime_UTR 45.0%
ACGTACCCCTCTTCTATCTT+GGG - Chr4:46834233-46834252 MsG0480020714.01.T01:three_prime_UTR 45.0%
ATCTCAAAGGACTCCAATGG+TGG + Chr4:46835049-46835068 None:intergenic 45.0%
CTGCTGAGAATAAGTACCAG+AGG - Chr4:46835209-46835228 MsG0480020714.01.T01:CDS 45.0%
GGAATCTCAAAGGACTCCAA+TGG + Chr4:46835052-46835071 None:intergenic 45.0%
GTAAGCCCAAGATAGAAGAG+GGG + Chr4:46834241-46834260 None:intergenic 45.0%
GTAATGGTCCTGCAAGCAAA+AGG + Chr4:46835006-46835025 None:intergenic 45.0%
GTCAATGGAGTACAATCCTC+TGG + Chr4:46835228-46835247 None:intergenic 45.0%
GTGAATGGAGGAAAAAGCCA+AGG + Chr4:46835556-46835575 None:intergenic 45.0%
GTGCTAGGTTAGGTAGTCAA+TGG + Chr4:46835243-46835262 None:intergenic 45.0%
TGGTTGAGGTGGTGAATGAA+TGG + Chr4:46835083-46835102 None:intergenic 45.0%
TTGCAGGACCATTACCTCAA+TGG - Chr4:46835011-46835030 MsG0480020714.01.T01:CDS 45.0%
! AAGGGGAGAAAAAGAGTGTG+AGG + Chr4:46834123-46834142 None:intergenic 45.0%
! AGCAAAAGGGTCTGAAGCAA+AGG + Chr4:46834992-46835011 None:intergenic 45.0%
! TTGGGGTTAAGGGTTTTCCT+TGG - Chr4:46835536-46835555 MsG0480020714.01.T01:intron 45.0%
!! TGTTCTTGTTTGTTGTGGGG+AGG + Chr4:46834879-46834898 None:intergenic 45.0%
!! AATGAAATTTATTAAAAAAA+TGG + Chr4:46834492-46834511 None:intergenic 5.0%
!! ATGAAATTTATTAAAAAAAT+GGG + Chr4:46834491-46834510 None:intergenic 5.0%
AGTTGTTGCTGCTGATGCTG+TGG + Chr4:46835106-46835125 None:intergenic 50.0%
GGCTTACTACTGCCAAGGAA+AGG - Chr4:46834254-46834273 MsG0480020714.01.T01:three_prime_UTR 50.0%
GGGTGTCTCCATTGAGGTAA+TGG + Chr4:46835022-46835041 None:intergenic 50.0%
GGTCACAGGTGGAATCTCAA+AGG + Chr4:46835062-46835081 None:intergenic 50.0%
GGTGAATGAATGGTCACAGG+TGG + Chr4:46835073-46835092 None:intergenic 50.0%
GGTGGTGAATGAATGGTCAC+AGG + Chr4:46835076-46835095 None:intergenic 50.0%
TCAAAGGACTCCAATGGTGG+TGG + Chr4:46835046-46835065 None:intergenic 50.0%
TCTTGGGCTTACTACTGCCA+AGG - Chr4:46834249-46834268 MsG0480020714.01.T01:three_prime_UTR 50.0%
TGGGAGAGAGACAGAGTTGT+GGG + Chr4:46834185-46834204 None:intergenic 50.0%
! CTTCAGACCCTTTTGCTTGC+AGG - Chr4:46834995-46835014 MsG0480020714.01.T01:CDS 50.0%
GTGGGAGAGAGACAGAGTTG+TGG + Chr4:46834186-46834205 None:intergenic 55.0%
TGATGCTGTGGTGGTTGAGG+TGG + Chr4:46835094-46835113 None:intergenic 55.0%
TGCTGATGCTGTGGTGGTTG+AGG + Chr4:46835097-46835116 None:intergenic 55.0%
TGTTGCTGCTGATGCTGTGG+TGG + Chr4:46835103-46835122 None:intergenic 55.0%
!! AAGGACTCCAATGGTGGTGG+AGG + Chr4:46835043-46835062 None:intergenic 55.0%
!! AGGACTCCAATGGTGGTGGA+GGG + Chr4:46835042-46835061 None:intergenic 55.0%
GAGACACCCTCCACCACCAT+TGG - Chr4:46835033-46835052 MsG0480020714.01.T01:CDS 60.0%
!! GGTGGAGGGTGTCTCCATTG+AGG + Chr4:46835028-46835047 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 46834112 46835819 46834112 ID=MsG0480020714.01;Name=MsG0480020714.01
Chr4 mRNA 46834112 46835819 46834112 ID=MsG0480020714.01.T01;Parent=MsG0480020714.01;Name=MsG0480020714.01.T01;_AED=0.10;_eAED=0.10;_QI=107|1|1|1|0|0|2|666|226
Chr4 exon 46834112 46835401 46834112 ID=MsG0480020714.01.T01:exon:2265;Parent=MsG0480020714.01.T01
Chr4 exon 46835656 46835819 46835656 ID=MsG0480020714.01.T01:exon:2264;Parent=MsG0480020714.01.T01
Chr4 five_prime_UTR 46835713 46835819 46835713 ID=MsG0480020714.01.T01:five_prime_utr;Parent=MsG0480020714.01.T01
Chr4 CDS 46835656 46835712 46835656 ID=MsG0480020714.01.T01:cds;Parent=MsG0480020714.01.T01
Chr4 CDS 46834778 46835401 46834778 ID=MsG0480020714.01.T01:cds;Parent=MsG0480020714.01.T01
Chr4 three_prime_UTR 46834112 46834777 46834112 ID=MsG0480020714.01.T01:three_prime_utr;Parent=MsG0480020714.01.T01
Gene Sequence

>MsG0480020714.01.T01

ATGGACAATCAACAACGTACCCCTCTTCTATCTTGGGCTTACTACTGCCAAGGAAAGTCAATGGATGAGTTGAAGCAAACCTTGATGTACACAACAATGGAGCTAGAACAAACAAGAGTAACAGTACAAGAAGAACTAAGAAAAAGAGATGAACAACTACATAACCTAAAAGAACTTTTCAACAAAGTTGTAAGAGAAAGAGATGAAGCACAAGAAAAATGCCAAAGACTTTTCTTAGAAAAGTTACTTTTCCAACAACAAAAACAACAACAAAACCAAGATCCTTTGTCTGGAATCTCAAGCATTGAAGATGAACAACAACAACAACAACAACAACAAAAGAGAGGAATTGAATCTTCCAACAATGGAATATCACTATCAGATTGTGAAGAAAGCATAGTATCCTCCCCACAACAAACAAGAACAGAACAACCAATGATGATGATTGATTCAATAGCAAAAGATAAACCTTTGCCAGAAAAAGGTAAACTTTTACAAGCAGTGATGAAAGCTGGTCCTTTGCTTCAGACCCTTTTGCTTGCAGGACCATTACCTCAATGGAGACACCCTCCACCACCATTGGAGTCCTTTGAGATTCCACCTGTGACCATTCATTCACCACCTCAACCACCACAGCATCAGCAGCAACAACTACTTCATCAAGAGTCCTTGTTAACATAA
Protein sequence

>MsG0480020714.01.T01

MDNQQRTPLLSWAYYCQGKSMDELKQTLMYTTMELEQTRVTVQEELRKRDEQLHNLKELFNKVVRERDEAQEKCQRLFLEKLLFQQQKQQQNQDPLSGISSIEDEQQQQQQQQKRGIESSNNGISLSDCEESIVSSPQQTRTEQPMMMIDSIAKDKPLPEKGKLLQAVMKAGPLLQTLLLAGPLPQWRHPPPPLESFEIPPVTIHSPPQPPQHQQQQLLHQESLLT*