AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048290.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048290.01.T01 MTR_4g057470 92.218 257 14 4 1 255 1 253 2.04e-155 432
MsG0080048290.01.T01 MTR_2g084270 62.264 265 61 11 1 253 1 238 4.17e-102 297
MsG0080048290.01.T01 MTR_1g090663 43.915 189 77 4 10 196 8 169 7.34e-43 145
MsG0080048290.01.T01 MTR_4g063070 35.268 224 91 11 10 201 4 205 4.52e-21 91.7
MsG0080048290.01.T01 MTR_2g096360 33.796 216 106 8 8 201 4 204 4.41e-20 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048290.01.T01 AT5G22930 53.788 264 85 8 1 254 1 237 1.49e-83 250
MsG0080048290.01.T01 AT3G44940 52.239 268 91 9 5 255 3 250 1.00e-66 207
MsG0080048290.01.T01 AT2G28140 44.186 258 91 7 1 253 1 210 5.62e-55 176
MsG0080048290.01.T01 AT2G28690 35.233 193 119 3 10 200 4 192 2.10e-29 110
MsG0080048290.01.T01 AT5G59760 33.838 198 115 5 9 201 4 190 2.94e-21 89.4

Find 81 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCGTCTGATCTTAAATTAA+TGG 0.229367 contig265end:-106261 MsG0080048290.01.T01:three_prime_UTR
GAAGATTCAATAGTAGAAAA+AGG 0.231209 contig265end:-106425 MsG0080048290.01.T01:CDS
GATAAACCTTTGCCAGAAAA+AGG 0.289825 contig265end:-106651 MsG0080048290.01.T01:CDS
TTTGGTAATGTTTGGGGTGA+AGG 0.321802 contig265end:-106052 MsG0080048290.01.T01:three_prime_UTR
GTTCTGTTCTTGCTTGTTGT+GGG 0.352565 contig265end:+106706 None:intergenic
TTGGTAATGTTTGGGGTGAA+GGG 0.367899 contig265end:-106051 MsG0080048290.01.T01:three_prime_UTR
AATGTAGTAGAGATCTTTAT+AGG 0.369590 contig265end:-105808 MsG0080048290.01.T01:three_prime_UTR
AATGAGAGGAAAAGAAGAAA+AGG 0.369977 contig265end:+107456 None:intergenic
AACGTACCCCTCTTCTATCT+TGG 0.371913 contig265end:-107377 MsG0080048290.01.T01:CDS
TTGGGGTGAAGGGGTTTCCT+TGG 0.373430 contig265end:-106041 MsG0080048290.01.T01:three_prime_UTR
ATGAGAGGAAAAGAAGAAAA+GGG 0.398562 contig265end:+107457 None:intergenic
TGGGAGAGAGACAGAGTTGT+GGG 0.399773 contig265end:+107427 None:intergenic
TGATAGTGATATTCCATTGT+TGG 0.406170 contig265end:+106755 None:intergenic
GAAGTGTTAGGAGTGTTATG+AGG 0.412710 contig265end:+107496 None:intergenic
ATTGAATCAATCATCATCAT+TGG 0.412861 contig265end:+106681 None:intergenic
TGCTGATGCTGTGGTGGTTG+AGG 0.414937 contig265end:+106492 None:intergenic
AGATTCCAGACAAAGGATCT+TGG 0.415576 contig265end:+106832 None:intergenic
TGTTCTGTTCTTGCTTGTTG+TGG 0.424690 contig265end:+106705 None:intergenic
TGTTTCTTTGTGAAAGGAAA+AGG 0.430527 contig265end:-105991 MsG0080048290.01.T01:three_prime_UTR
CTCCATTGTTGTGTACATCA+AGG 0.444003 contig265end:+107028 None:intergenic
ACGTACCCCTCTTCTATCTT+GGG 0.445573 contig265end:-107376 MsG0080048290.01.T01:CDS
GCAAGTGATTTGAGTGTGTA+TGG 0.451748 contig265end:-105925 MsG0080048290.01.T01:three_prime_UTR
GGGTGTCTCCATTGAGGTAA+TGG 0.451885 contig265end:+106567 None:intergenic
TCCCACTCTTTCTAAGATCA+TGG 0.456841 contig265end:-107409 MsG0080048290.01.T01:exon
ATGAGGGATGGTTGAATTGA+AGG 0.457056 contig265end:+107513 None:intergenic
AAGTGTTAGGAGTGTTATGA+GGG 0.458011 contig265end:+107497 None:intergenic
AAAAGAGTGTTAGAAGTGTT+AGG 0.460549 contig265end:+107484 None:intergenic
GTAATGGTCCTGCAAGCAAA+AGG 0.461418 contig265end:+106583 None:intergenic
CAAAACCAAGATCCTTTGTC+TGG 0.462496 contig265end:-106837 MsG0080048290.01.T01:CDS
TGGTAATGTTTGGGGTGAAG+GGG 0.465304 contig265end:-106050 MsG0080048290.01.T01:three_prime_UTR
CTGATCTAGCTTATGTATAG+TGG 0.465653 contig265end:-106112 MsG0080048290.01.T01:three_prime_UTR
AAGAAGTAGGTACCTTTCCT+TGG 0.473902 contig265end:+107343 None:intergenic
TAGTAACAGTGCTAGGTACT+AGG 0.474829 contig265end:+106333 None:intergenic
AATGATTAGCATTAGCAAGA+TGG 0.475888 contig265end:-106142 MsG0080048290.01.T01:three_prime_UTR
AAAGACTCCAATGGTGGTGG+AGG 0.485852 contig265end:+106546 None:intergenic
AAACAAAACTAGAATGTGAA+TGG 0.487451 contig265end:+106009 None:intergenic
ACCGTTAGAATTAGTTCTAT+CGG 0.488516 contig265end:-105857 MsG0080048290.01.T01:three_prime_UTR
GTCAATGGAGTACAATCCTC+TGG 0.508694 contig265end:+106358 None:intergenic
GTGGGAGAGAGACAGAGTTG+TGG 0.509113 contig265end:+107426 None:intergenic
GCTAGGTACTAGGTAGTCAA+TGG 0.512664 contig265end:+106343 None:intergenic
GTCCATGATCTTAGAAAGAG+TGG 0.515664 contig265end:+107407 None:intergenic
TTCTGTTCTTGCTTGTTGTG+GGG 0.515975 contig265end:+106707 None:intergenic
TGGTTGAGGTGGTGAATGAA+TGG 0.519132 contig265end:+106506 None:intergenic
ACCATTAATTTAAGATCAGA+CGG 0.523566 contig265end:+106260 None:intergenic
GAGACACCCTCCACCACCAT+TGG 0.528054 contig265end:-106553 MsG0080048290.01.T01:CDS
TTGCAGGACCATTACCTCAA+TGG 0.529726 contig265end:-106575 MsG0080048290.01.T01:CDS
TGTTCTTGCTTGTTGTGGGG+AGG 0.535282 contig265end:+106710 None:intergenic
TTACAAGCAGTGATGAAAGC+TGG 0.538923 contig265end:-106621 MsG0080048290.01.T01:CDS
AGCAAAAGGGTCTGAAGCAA+AGG 0.539159 contig265end:+106597 None:intergenic
TAGCTTATGTATAGTGGTGG+AGG 0.541339 contig265end:-106106 MsG0080048290.01.T01:three_prime_UTR
TGAGAGGAAAAGAAGAAAAG+GGG 0.547075 contig265end:+107458 None:intergenic
TCAAAAGACTCCAATGGTGG+TGG 0.547170 contig265end:+106543 None:intergenic
GGAATTGAATCTTCCAACAA+TGG 0.550626 contig265end:-106768 MsG0080048290.01.T01:CDS
AGTAAGCCCAAGATAGAAGA+GGG 0.552955 contig265end:+107370 None:intergenic
TGTTGCTGCTGATGCTGTGG+TGG 0.555209 contig265end:+106486 None:intergenic
TGATGCTGTGGTGGTTGAGG+TGG 0.562813 contig265end:+106495 None:intergenic
CTTCATTCAACAAGTACTAG+AGG 0.563006 contig265end:-106306 MsG0080048290.01.T01:three_prime_UTR
AAGTCAATAGTAACAGTGCT+AGG 0.563845 contig265end:+106326 None:intergenic
GTTAGGAGTGTTATGAGGGA+TGG 0.569753 contig265end:+107501 None:intergenic
TAATGGTCCTGCAAGCAAAA+GGG 0.574855 contig265end:+106584 None:intergenic
AGTTGTTGCTGCTGATGCTG+TGG 0.582168 contig265end:+106483 None:intergenic
GGTGGAGGGTGTCTCCATTG+AGG 0.584272 contig265end:+106561 None:intergenic
TGGTACTTATTCTCAGCAGA+AGG 0.589538 contig265end:+106378 None:intergenic
GTTGTGGGAGAGAAAATGAG+AGG 0.600479 contig265end:+107442 None:intergenic
ACCGATAGAACTAATTCTAA+CGG 0.602859 contig265end:+105856 None:intergenic
ATCTAGCTTATGTATAGTGG+TGG 0.604531 contig265end:-106109 MsG0080048290.01.T01:three_prime_UTR
CAAGTGATTTGAGTGTGTAT+GGG 0.615188 contig265end:-105924 MsG0080048290.01.T01:three_prime_UTR
ATGCTTGAGATTCCAGACAA+AGG 0.616707 contig265end:+106825 None:intergenic
TCTTGGGCTTACTACTGCCA+AGG 0.616963 contig265end:-107360 MsG0080048290.01.T01:CDS
TAGTAAGCCCAAGATAGAAG+AGG 0.618226 contig265end:+107369 None:intergenic
GGCTTACTACTGCCAAGGAA+AGG 0.619012 contig265end:-107355 MsG0080048290.01.T01:intron
AACCTTGATGTACACAACAA+TGG 0.621287 contig265end:-107030 MsG0080048290.01.T01:CDS
AAGACTCCAATGGTGGTGGA+GGG 0.621423 contig265end:+106547 None:intergenic
GGAATCTCAAAAGACTCCAA+TGG 0.630030 contig265end:+106537 None:intergenic
CTGCTGAGAATAAGTACCAG+AGG 0.635351 contig265end:-106374 MsG0080048290.01.T01:CDS
CAAAACTAGAATGTGAATGG+AGG 0.644567 contig265end:+106012 None:intergenic
ATCTCAAAAGACTCCAATGG+TGG 0.646695 contig265end:+106540 None:intergenic
GGTGGTGAATGAATGGTCAC+AGG 0.654394 contig265end:+106513 None:intergenic
TCCATGATCTTAGAAAGAGT+GGG 0.664683 contig265end:+107408 None:intergenic
GGTGAATGAATGGTCACAGG+TGG 0.686774 contig265end:+106516 None:intergenic
GTAAGCCCAAGATAGAAGAG+GGG 0.693101 contig265end:+107371 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAAATTCTTGATTTT+GGG - contig265end:107239-107258 MsG0080048290.01.T01:intron 10.0%
!! ATATCAATTAAAAACTAAGT+TGG + contig265end:107119-107138 None:intergenic 15.0%
!! TATAAAATACTTGTAATTTG+AGG - contig265end:106043-106062 MsG0080048290.01.T01:three_prime_UTR 15.0%
!!! GAAAAAAAAATTCTTGATTT+TGG - contig265end:107238-107257 MsG0080048290.01.T01:intron 15.0%
!!! GTTAGTCTAATAAAATTAAA+AGG + contig265end:106120-106139 None:intergenic 15.0%
!!! GTTTTTGTAATATAATTGTA+AGG - contig265end:107347-107366 MsG0080048290.01.T01:intron 15.0%
!! AAGATTCAATAGTAGAAAAA+GGG - contig265end:106892-106911 MsG0080048290.01.T01:CDS 20.0%
!!! AAAAACTAAGTTGGTAAAAT+TGG + contig265end:107110-107129 None:intergenic 20.0%
!!! AGTTAGTCTTATAAACATTT+TGG + contig265end:106082-106101 None:intergenic 20.0%
!!! ATAGGATTTGAAGTTAAAAA+AGG + contig265end:106176-106195 None:intergenic 20.0%
!!! TAGGATTTGAAGTTAAAAAA+GGG + contig265end:106175-106194 None:intergenic 20.0%
!!! TCTTCATAGTTACATTTTAT+AGG - contig265end:106018-106037 MsG0080048290.01.T01:three_prime_UTR 20.0%
!!! TTATAAACATTTTGGATGAA+TGG + contig265end:106074-106093 None:intergenic 20.0%
! AAACAAAACTAGAATGTGAA+TGG + contig265end:107310-107329 None:intergenic 25.0%
! AATGTAGTAGAGATCTTTAT+AGG - contig265end:107508-107527 MsG0080048290.01.T01:five_prime_UTR 25.0%
! ATTAGACTAACTTCAAATGT+GGG - contig265end:106129-106148 MsG0080048290.01.T01:three_prime_UTR 25.0%
! ATTGAATCAATCATCATCAT+TGG + contig265end:106638-106657 None:intergenic 25.0%
! GAAGATTCAATAGTAGAAAA+AGG - contig265end:106891-106910 MsG0080048290.01.T01:CDS 25.0%
! TATTAGACTAACTTCAAATG+TGG - contig265end:106128-106147 MsG0080048290.01.T01:three_prime_UTR 25.0%
! TGTAATGAAAGAAAAGAAGT+AGG + contig265end:105989-106008 None:intergenic 25.0%
!! AATTTTTGTGATACAGTCAA+TGG - contig265end:106250-106269 MsG0080048290.01.T01:three_prime_UTR 25.0%
!! ACCATTAATTTAAGATCAGA+CGG + contig265end:107059-107078 None:intergenic 25.0%
!! AGTACTAGAGGATTTTATTA+CGG - contig265end:107022-107041 MsG0080048290.01.T01:CDS 25.0%
!! CACTAATGATCATTTTACAA+CGG + contig265end:106207-106226 None:intergenic 25.0%
!! CTTTGTTGAAAAGTTCTTTT+AGG + contig265end:106376-106395 None:intergenic 25.0%
!! TGTAAAAGTTTACCTTTTTC+TGG + contig265end:106680-106699 None:intergenic 25.0%
!!! AATTCTTGATTTTGGGTTTT+TGG - contig265end:107246-107265 MsG0080048290.01.T01:intron 25.0%
!!! ACTTTTCTAAGAAAAGTCTT+TGG + contig265end:106433-106452 None:intergenic 25.0%
!!! GATTTTTTATTATTGTTGCG+CGG - contig265end:107420-107439 MsG0080048290.01.T01:five_prime_UTR 25.0%
!!! TAGTTTTGTTTCTTTGTGAA+AGG - contig265end:107319-107338 MsG0080048290.01.T01:intron 25.0%
!!! TTTGTTGTTGTTTTTGTTGT+TGG + contig265end:106463-106482 None:intergenic 25.0%
AATGAGAGGAAAAGAAGAAA+AGG + contig265end:105863-105882 None:intergenic 30.0%
AATGATTAGCATTAGCAAGA+TGG - contig265end:107174-107193 MsG0080048290.01.T01:intron 30.0%
ACCGATAGAACTAATTCTAA+CGG + contig265end:107463-107482 None:intergenic 30.0%
ACCGTCTGATCTTAAATTAA+TGG - contig265end:107055-107074 MsG0080048290.01.T01:intron 30.0%
ATGAGAGGAAAAGAAGAAAA+GGG + contig265end:105862-105881 None:intergenic 30.0%
TGTTTCTTTGTGAAAGGAAA+AGG - contig265end:107325-107344 MsG0080048290.01.T01:intron 30.0%
! AAACATTTTGGATGAATGGT+TGG + contig265end:106070-106089 None:intergenic 30.0%
! TCTTTTGTTAACAGTAACTG+TGG - contig265end:106869-106888 MsG0080048290.01.T01:CDS 30.0%
! TGATAGTGATATTCCATTGT+TGG + contig265end:106564-106583 None:intergenic 30.0%
!! AAAAGAGTGTTAGAAGTGTT+AGG + contig265end:105835-105854 None:intergenic 30.0%
!! ACCGTTAGAATTAGTTCTAT+CGG - contig265end:107459-107478 MsG0080048290.01.T01:five_prime_UTR 30.0%
!!! AGAAAAAGGGTTTTTTGTGA+AGG - contig265end:106905-106924 MsG0080048290.01.T01:CDS 30.0%
!!! TTGGGTTTTTGGTAATGTTT+GGG - contig265end:107257-107276 MsG0080048290.01.T01:intron 30.0%
!!! TTTGGGTTTTTGGTAATGTT+TGG - contig265end:107256-107275 MsG0080048290.01.T01:intron 30.0%
AACCTTGATGTACACAACAA+TGG - contig265end:106286-106305 MsG0080048290.01.T01:three_prime_UTR 35.0%
AAGTCAATAGTAACAGTGCT+AGG + contig265end:106993-107012 None:intergenic 35.0%
CAAAACTAGAATGTGAATGG+AGG + contig265end:107307-107326 None:intergenic 35.0%
CAACAACAACAACAAAAGAG+AGG - contig265end:106527-106546 MsG0080048290.01.T01:CDS 35.0%
CAAGTGATTTGAGTGTGTAT+GGG - contig265end:107392-107411 MsG0080048290.01.T01:CDS 35.0%
CTTCATTCAACAAGTACTAG+AGG - contig265end:107010-107029 MsG0080048290.01.T01:CDS 35.0%
GATAAACCTTTGCCAGAAAA+AGG - contig265end:106665-106684 MsG0080048290.01.T01:CDS 35.0%
GGAATTGAATCTTCCAACAA+TGG - contig265end:106548-106567 MsG0080048290.01.T01:CDS 35.0%
TATGTCAGAGAGATTTCGAT+TGG - contig265end:107535-107554 MsG0080048290.01.T01:five_prime_UTR 35.0%
TCCATGATCTTAGAAAGAGT+GGG + contig265end:105911-105930 None:intergenic 35.0%
TGAGAGGAAAAGAAGAAAAG+GGG + contig265end:105861-105880 None:intergenic 35.0%
TTTACAACGGTTTCTCAGAT+AGG + contig265end:106194-106213 None:intergenic 35.0%
! ATCTAGCTTATGTATAGTGG+TGG - contig265end:107207-107226 MsG0080048290.01.T01:intron 35.0%
! CTGATCTAGCTTATGTATAG+TGG - contig265end:107204-107223 MsG0080048290.01.T01:intron 35.0%
!! AAGTGTTAGGAGTGTTATGA+GGG + contig265end:105822-105841 None:intergenic 35.0%
!! AGTTTACCTTTTTCTGGCAA+AGG + contig265end:106674-106693 None:intergenic 35.0%
!!! TGGGTTTTTGGTAATGTTTG+GGG - contig265end:107258-107277 MsG0080048290.01.T01:intron 35.0%
AAGAAGTAGGTACCTTTCCT+TGG + contig265end:105976-105995 None:intergenic 40.0%
AGATTCCAGACAAAGGATCT+TGG + contig265end:106487-106506 None:intergenic 40.0%
AGTAAGCCCAAGATAGAAGA+GGG + contig265end:105949-105968 None:intergenic 40.0%
ATCTCAAAAGACTCCAATGG+TGG + contig265end:106779-106798 None:intergenic 40.0%
ATGAGGGATGGTTGAATTGA+AGG + contig265end:105806-105825 None:intergenic 40.0%
ATGCTTGAGATTCCAGACAA+AGG + contig265end:106494-106513 None:intergenic 40.0%
CAAAACCAAGATCCTTTGTC+TGG - contig265end:106479-106498 MsG0080048290.01.T01:CDS 40.0%
CTCCATTGTTGTGTACATCA+AGG + contig265end:106291-106310 None:intergenic 40.0%
GAAGTTAAAAAAGGGCAACC+CGG + contig265end:106167-106186 None:intergenic 40.0%
GCAAGTGATTTGAGTGTGTA+TGG - contig265end:107391-107410 MsG0080048290.01.T01:CDS 40.0%
GGAATCTCAAAAGACTCCAA+TGG + contig265end:106782-106801 None:intergenic 40.0%
GTCCATGATCTTAGAAAGAG+TGG + contig265end:105912-105931 None:intergenic 40.0%
TAATGGTCCTGCAAGCAAAA+GGG + contig265end:106735-106754 None:intergenic 40.0%
TAGCTTATGTATAGTGGTGG+AGG - contig265end:107210-107229 MsG0080048290.01.T01:intron 40.0%
TAGTAACAGTGCTAGGTACT+AGG + contig265end:106986-107005 None:intergenic 40.0%
TAGTAAGCCCAAGATAGAAG+AGG + contig265end:105950-105969 None:intergenic 40.0%
TGGTACTTATTCTCAGCAGA+AGG + contig265end:106941-106960 None:intergenic 40.0%
TTACAAGCAGTGATGAAAGC+TGG - contig265end:106695-106714 MsG0080048290.01.T01:CDS 40.0%
! TGTTCTGTTCTTGCTTGTTG+TGG + contig265end:106614-106633 None:intergenic 40.0%
!! GAAGTGTTAGGAGTGTTATG+AGG + contig265end:105823-105842 None:intergenic 40.0%
!! GTTCTGTTCTTGCTTGTTGT+GGG + contig265end:106613-106632 None:intergenic 40.0%
!! TCCCACTCTTTCTAAGATCA+TGG - contig265end:105907-105926 MsG0080048290.01.T01:three_prime_UTR 40.0%
!! TTCTGTTCTTGCTTGTTGTG+GGG + contig265end:106612-106631 None:intergenic 40.0%
!! TTGGTAATGTTTGGGGTGAA+GGG - contig265end:107265-107284 MsG0080048290.01.T01:intron 40.0%
!! TTTGGTAATGTTTGGGGTGA+AGG - contig265end:107264-107283 MsG0080048290.01.T01:intron 40.0%
AACGTACCCCTCTTCTATCT+TGG - contig265end:105939-105958 MsG0080048290.01.T01:three_prime_UTR 45.0%
ACGTACCCCTCTTCTATCTT+GGG - contig265end:105940-105959 MsG0080048290.01.T01:three_prime_UTR 45.0%
CTGCTGAGAATAAGTACCAG+AGG - contig265end:106942-106961 MsG0080048290.01.T01:CDS 45.0%
GCTAGGTACTAGGTAGTCAA+TGG + contig265end:106976-106995 None:intergenic 45.0%
GTAAGCCCAAGATAGAAGAG+GGG + contig265end:105948-105967 None:intergenic 45.0%
GTAATGGTCCTGCAAGCAAA+AGG + contig265end:106736-106755 None:intergenic 45.0%
GTCAATGGAGTACAATCCTC+TGG + contig265end:106961-106980 None:intergenic 45.0%
GTGAATGGAGGAAAAAGCCA+AGG + contig265end:107295-107314 None:intergenic 45.0%
GTTGTGGGAGAGAAAATGAG+AGG + contig265end:105877-105896 None:intergenic 45.0%
TCAAAAGACTCCAATGGTGG+TGG + contig265end:106776-106795 None:intergenic 45.0%
TGGTTGAGGTGGTGAATGAA+TGG + contig265end:106813-106832 None:intergenic 45.0%
TTGCAGGACCATTACCTCAA+TGG - contig265end:106741-106760 MsG0080048290.01.T01:CDS 45.0%
! AGCAAAAGGGTCTGAAGCAA+AGG + contig265end:106722-106741 None:intergenic 45.0%
! TGGTAATGTTTGGGGTGAAG+GGG - contig265end:107266-107285 MsG0080048290.01.T01:intron 45.0%
!! GTTAGGAGTGTTATGAGGGA+TGG + contig265end:105818-105837 None:intergenic 45.0%
AGTTGTTGCTGCTGATGCTG+TGG + contig265end:106836-106855 None:intergenic 50.0%
GGCTTACTACTGCCAAGGAA+AGG - contig265end:105961-105980 MsG0080048290.01.T01:three_prime_UTR 50.0%
GGGTGTCTCCATTGAGGTAA+TGG + contig265end:106752-106771 None:intergenic 50.0%
GGTGAATGAATGGTCACAGG+TGG + contig265end:106803-106822 None:intergenic 50.0%
GGTGGTGAATGAATGGTCAC+AGG + contig265end:106806-106825 None:intergenic 50.0%
TCTTGGGCTTACTACTGCCA+AGG - contig265end:105956-105975 MsG0080048290.01.T01:three_prime_UTR 50.0%
TGGGAGAGAGACAGAGTTGT+GGG + contig265end:105892-105911 None:intergenic 50.0%
! ATGTGGGAGCTTTAGTGCAC+CGG - contig265end:106145-106164 MsG0080048290.01.T01:three_prime_UTR 50.0%
! CTTCAGACCCTTTTGCTTGC+AGG - contig265end:106725-106744 MsG0080048290.01.T01:CDS 50.0%
!! AAAGACTCCAATGGTGGTGG+AGG + contig265end:106773-106792 None:intergenic 50.0%
!! AAGACTCCAATGGTGGTGGA+GGG + contig265end:106772-106791 None:intergenic 50.0%
!! TGTTCTTGCTTGTTGTGGGG+AGG + contig265end:106609-106628 None:intergenic 50.0%
GTGGGAGAGAGACAGAGTTG+TGG + contig265end:105893-105912 None:intergenic 55.0%
TGATGCTGTGGTGGTTGAGG+TGG + contig265end:106824-106843 None:intergenic 55.0%
TGCTGATGCTGTGGTGGTTG+AGG + contig265end:106827-106846 None:intergenic 55.0%
TGTTGCTGCTGATGCTGTGG+TGG + contig265end:106833-106852 None:intergenic 55.0%
TTGGGGTGAAGGGGTTTCCT+TGG - contig265end:107275-107294 MsG0080048290.01.T01:intron 55.0%
! TGTGGGAGCTTTAGTGCACC+GGG - contig265end:106146-106165 MsG0080048290.01.T01:three_prime_UTR 55.0%
GAGACACCCTCCACCACCAT+TGG - contig265end:106763-106782 MsG0080048290.01.T01:CDS 60.0%
!! GGTGGAGGGTGTCTCCATTG+AGG + contig265end:106758-106777 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
contig265end gene 105783 107555 105783 ID=MsG0080048290.01;Name=MsG0080048290.01
contig265end mRNA 105783 107555 105783 ID=MsG0080048290.01.T01;Parent=MsG0080048290.01;Name=MsG0080048290.01.T01;_AED=0.18;_eAED=0.18;_QI=143|0|0.5|1|0|0.5|2|576|255
contig265end exon 105783 107069 105783 ID=MsG0080048290.01.T01:exon:27825;Parent=MsG0080048290.01.T01
contig265end exon 107356 107555 107356 ID=MsG0080048290.01.T01:exon:27824;Parent=MsG0080048290.01.T01
contig265end five_prime_UTR 107413 107555 107413 ID=MsG0080048290.01.T01:five_prime_utr;Parent=MsG0080048290.01.T01
contig265end CDS 107356 107412 107356 ID=MsG0080048290.01.T01:cds;Parent=MsG0080048290.01.T01
contig265end CDS 106359 107069 106359 ID=MsG0080048290.01.T01:cds;Parent=MsG0080048290.01.T01
contig265end three_prime_UTR 105783 106358 105783 ID=MsG0080048290.01.T01:three_prime_utr;Parent=MsG0080048290.01.T01
Gene Sequence

>MsG0080048290.01.T01

ATGGACAATCAACAACGTACCCCTCTTCTATCTTGGGCTTACTACTGCCAAGGAAAGATGGATGAGTTGAAGCAAACCTTGATGTACACAACAATGGAGCTAGAACAAACAAGAGTAACAGTACAAGAAGAACTAAGAAAAAGAGATGAACAACTACATAACCTAAAAGAACTTTTCAACAAAGTTGTACGAGAAAGAGATGAAGCACAAGAAAAATGCCAAAGACTTTTCTTAGAAAAGTTACTTTTCCAACAACAAAAACAACAACAAAACCAAGATCCTTTGTCTGGAATCTCAAGCATTGAAGATGAACAACAACAACAACAACAAAAGAGAGGAATTGAATCTTCCAACAATGGAATATCACTATCAGATTGTGAAGAAAGCATAGTATCCTCCCCACAACAAGCAAGAACAGAACAACCAATGATGATGATTGATTCAATAGCAAAAGATAAACCTTTGCCAGAAAAAGGTAAACTTTTACAAGCAGTGATGAAAGCTGGTCCTTTGCTTCAGACCCTTTTGCTTGCAGGACCATTACCTCAATGGAGACACCCTCCACCACCATTGGAGTCTTTTGAGATTCCACCTGTGACCATTCATTCACCACCTCAACCACCACAGCATCAGCAGCAACAACTACTTCATCAAGAGTCTTTTGTTAACAGTAACTGTGGAAGATTCAATAGTAGAAAAAGGGTTTTTTGTGAAGGTTCTGATTCTCCTTCTGCTGAGAATAAGTACCAGAGGATTGTACTCCATTGA
Protein sequence

>MsG0080048290.01.T01

MDNQQRTPLLSWAYYCQGKMDELKQTLMYTTMELEQTRVTVQEELRKRDEQLHNLKELFNKVVRERDEAQEKCQRLFLEKLLFQQQKQQQNQDPLSGISSIEDEQQQQQQKRGIESSNNGISLSDCEESIVSSPQQARTEQPMMMIDSIAKDKPLPEKGKLLQAVMKAGPLLQTLLLAGPLPQWRHPPPPLESFEIPPVTIHSPPQPPQHQQQQLLHQESFVNSNCGRFNSRKRVFCEGSDSPSAENKYQRIVLH*