AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020858.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020858.01.T01 MTR_4g061140 99.187 246 2 0 1 246 1 246 0.0 498
MsG0480020858.01.T01 MTR_4g061140 99.187 246 2 0 1 246 1 246 0.0 498
MsG0480020858.01.T01 MTR_4g061140 96.226 212 8 0 35 246 1 212 1.12e-150 419
MsG0480020858.01.T01 MTR_5g022510 64.017 239 85 1 8 246 6 243 1.25e-112 325
MsG0480020858.01.T01 MTR_4g073410 62.917 240 88 1 7 246 6 244 3.25e-108 314
MsG0480020858.01.T01 MTR_5g022510 64.078 206 73 1 41 246 14 218 1.55e-95 280
MsG0480020858.01.T01 MTR_5g008440 81.437 167 17 1 80 246 29 181 4.36e-91 267
MsG0480020858.01.T01 MTR_3g088160 46.094 256 112 6 16 246 101 355 6.97e-68 214
MsG0480020858.01.T01 MTR_3g088160 46.094 256 112 6 16 246 101 355 1.45e-67 215
MsG0480020858.01.T01 MTR_3g107060 39.407 236 120 7 20 246 93 314 2.90e-40 141
MsG0480020858.01.T01 MTR_4g122640 30.047 213 105 8 73 246 112 319 9.50e-15 72.8
MsG0480020858.01.T01 MTR_8g087680 68.293 41 13 0 66 106 32 72 1.01e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020858.01.T01 AT1G07890 80.894 246 47 0 1 246 1 246 1.91e-153 427
MsG0480020858.01.T01 AT1G07890 80.894 246 47 0 1 246 1 246 2.36e-153 427
MsG0480020858.01.T01 AT1G07890 80.894 246 47 0 1 246 1 246 2.36e-153 427
MsG0480020858.01.T01 AT1G07890 80.894 246 47 0 1 246 1 246 2.36e-153 427
MsG0480020858.01.T01 AT1G07890 80.894 246 47 0 1 246 1 246 2.36e-153 427
MsG0480020858.01.T01 AT1G07890 80.894 246 47 0 1 246 1 246 2.36e-153 427
MsG0480020858.01.T01 AT1G07890 80.894 246 47 0 1 246 1 246 2.36e-153 427
MsG0480020858.01.T01 AT1G07890 80.894 246 47 0 1 246 1 246 2.36e-153 427
MsG0480020858.01.T01 AT3G09640 81.557 244 44 1 3 246 4 246 1.51e-147 412
MsG0480020858.01.T01 AT3G09640 81.557 244 44 1 3 246 4 246 1.51e-147 412
MsG0480020858.01.T01 AT4G35000 66.805 241 79 1 6 246 4 243 1.59e-115 332
MsG0480020858.01.T01 AT4G35970 60.000 240 94 2 7 246 4 241 2.10e-99 291
MsG0480020858.01.T01 AT4G35970 59.361 219 87 2 7 225 4 220 2.39e-89 263
MsG0480020858.01.T01 AT1G77490 45.865 266 118 5 6 246 76 340 8.29e-68 215
MsG0480020858.01.T01 AT1G77490 45.865 266 118 5 6 246 76 340 1.04e-67 215
MsG0480020858.01.T01 AT4G08390 46.484 256 111 6 16 246 107 361 1.00e-65 208
MsG0480020858.01.T01 AT4G08390 46.484 256 111 6 16 246 107 361 1.00e-65 208
MsG0480020858.01.T01 AT4G08390 46.484 256 111 6 16 246 107 361 1.00e-65 208
MsG0480020858.01.T01 AT4G08390 46.484 256 111 6 16 246 106 360 1.03e-65 208
MsG0480020858.01.T01 AT4G08390 42.969 256 94 8 16 246 107 335 1.09e-53 177
MsG0480020858.01.T01 AT4G32320 36.653 251 125 7 5 246 98 323 1.44e-38 137
MsG0480020858.01.T01 AT4G31760 32.663 199 84 7 91 246 118 309 2.38e-17 80.1
MsG0480020858.01.T01 AT3G28200 26.882 279 155 10 12 246 26 299 1.97e-14 72.0
MsG0480020858.01.T01 AT4G33420 32.474 194 96 8 82 245 119 307 9.36e-14 70.1
MsG0480020858.01.T01 AT4G09010 27.519 258 110 8 28 246 43 262 1.21e-13 69.3
MsG0480020858.01.T01 AT4G33420 32.474 194 96 8 82 245 166 354 1.37e-13 69.7
MsG0480020858.01.T01 AT4G09010 27.519 258 110 8 28 246 108 327 1.94e-13 69.3
MsG0480020858.01.T01 AT4G09010 27.519 258 110 8 28 246 155 374 4.03e-13 68.6

Find 89 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGTTGCTGTTGAGATTAC+TGG 0.232804 4:-49730807 MsG0480020858.01.T01:CDS
TGATATCGCTGTTAGGCTTT+TGG 0.241502 4:-49730979 MsG0480020858.01.T01:CDS
GCTCTCCTCGTGTCACTGTT+AGG 0.263578 4:-49731882 MsG0480020858.01.T01:five_prime_UTR
TCGCCTTCTCAACAGCCTTT+TGG 0.306455 4:+49731577 None:intergenic
TCGAAATCTAAGAAGGAAAA+TGG 0.317014 4:+49731844 None:intergenic
AAGCTCAGCTTGGTGCTTGA+TGG 0.336855 4:+49731025 None:intergenic
TCAACAAGTGGGCGGAATAC+AGG 0.362317 4:+49729372 None:intergenic
CGCTCCTCTTATGCTCCGTT+TGG 0.364102 4:-49731517 MsG0480020858.01.T01:CDS
ACAAAGACTGGTGGTCCTTT+CGG 0.381599 4:-49731050 MsG0480020858.01.T01:CDS
CGACCCTCTGGTGGTGGCTC+AGG 0.382379 4:+49730134 None:intergenic
GCTGCACACAAGGAGCGTTC+TGG 0.387014 4:-49729613 MsG0480020858.01.T01:CDS
CAGGACATCGTTGCTCTGTC+TGG 0.408589 4:-49729955 MsG0480020858.01.T01:CDS
TCTTCAGTTGCCAAGTGATA+AGG 0.411660 4:-49729409 MsG0480020858.01.T01:CDS
AAATCAGCATAGCTAATGAT+TGG 0.419318 4:+49730936 None:intergenic
ACGGTCTTGATATCGCTGTT+AGG 0.422947 4:-49730986 MsG0480020858.01.T01:CDS
ACACGCTCTTTCTTCACTCT+TGG 0.424674 4:-49731910 None:intergenic
TGCAGGTTCTGACCATTTGA+GGG 0.426677 4:-49730010 MsG0480020858.01.T01:intron
TTGTGTAGATGGCACTCTGC+TGG 0.426968 4:-49731089 MsG0480020858.01.T01:intron
TGAAATGATTAGGGAGTTGT+TGG 0.432563 4:-49729451 MsG0480020858.01.T01:intron
CACTAACCCTTGGTGGCATC+AGG 0.433823 4:+49730107 None:intergenic
CCGGGGTGGAAAGGAACTTC+AGG 0.436434 4:+49730780 None:intergenic
CTCTCCTCGTGTCACTGTTA+GGG 0.437435 4:-49731881 MsG0480020858.01.T01:five_prime_UTR
GGATGTGTTTGGCAAAGCTA+TGG 0.443040 4:-49729989 MsG0480020858.01.T01:CDS
ATTGGGAATTGCTCCTTAAG+AGG 0.447187 4:+49730954 None:intergenic
TTTGATTCAAAGACAAAGAC+TGG 0.447588 4:-49731062 MsG0480020858.01.T01:CDS
CAAGGAGCGTTCTGGATTTG+AGG 0.450558 4:-49729605 MsG0480020858.01.T01:CDS
GGAGTTGTTGGGTGGTGAGA+AGG 0.456331 4:-49729439 MsG0480020858.01.T01:CDS
TTGCAGGTTCTGACCATTTG+AGG 0.456831 4:-49730011 MsG0480020858.01.T01:intron
GGCAAGCGACCCTCTGGTGG+TGG 0.457136 4:+49730128 None:intergenic
GGTAATCAGCACTCACGGTT+GGG 0.463922 4:+49731598 None:intergenic
GCATCAGGCAAGCGACCCTC+TGG 0.466554 4:+49730122 None:intergenic
GACCATTTGAGGGATGTGTT+TGG 0.471367 4:-49730000 MsG0480020858.01.T01:CDS
ATCAAAAGCTATCTGAGCTT+GGG 0.472564 4:-49729185 MsG0480020858.01.T01:CDS
CATCAAAAGCTATCTGAGCT+TGG 0.474904 4:-49729186 MsG0480020858.01.T01:CDS
CTCAAAAGTGCCTTATCACT+TGG 0.477819 4:+49729399 None:intergenic
TACGCCAAACGGAGCATAAG+AGG 0.478986 4:+49731513 None:intergenic
TTTGTGGTTATGCAGTTGGC+TGG 0.479033 4:-49730831 MsG0480020858.01.T01:intron
AAAATAAAACTTACGCCAAA+CGG 0.488936 4:+49731502 None:intergenic
TATCACTTGGCAACTGAAGA+AGG 0.489890 4:+49729412 None:intergenic
CACCATGAGCAAGCTCAGCT+TGG 0.497717 4:+49731015 None:intergenic
GATGTGTTTGGCAAAGCTAT+GGG 0.498498 4:-49729988 MsG0480020858.01.T01:CDS
AATCAGCATAGCTAATGATT+GGG 0.504612 4:+49730937 None:intergenic
TGCCAAACACATCCCTCAAA+TGG 0.508400 4:+49729998 None:intergenic
AAGGAGCGTTCTGGATTTGA+GGG 0.511703 4:-49729604 MsG0480020858.01.T01:CDS
CACCAAGCTGAGCTTGCTCA+TGG 0.514212 4:-49731017 MsG0480020858.01.T01:CDS
AGCTATGGGGCTTAGTGACC+AGG 0.514461 4:-49729974 MsG0480020858.01.T01:CDS
TAGTAAACGTACCTCCCTAC+CGG 0.522438 4:+49730761 None:intergenic
AAGTTCCTTTCCACCCCGGT+AGG 0.526103 4:-49730776 MsG0480020858.01.T01:CDS
ATGAGAGGATTAGAAGTCCA+TGG 0.528007 4:+49729580 None:intergenic
CAACAAGTGGGCGGAATACA+GGG 0.530888 4:+49729373 None:intergenic
ACCTCCCTACCGGGGTGGAA+AGG 0.535670 4:+49730771 None:intergenic
AGTAAACGTACCTCCCTACC+GGG 0.538079 4:+49730762 None:intergenic
CGTGAGTGCTGATTACCAAA+AGG 0.551875 4:-49731592 MsG0480020858.01.T01:CDS
CAGACAGAGCAACGATGTCC+TGG 0.552668 4:+49729956 None:intergenic
TCCAGAACACTAACCCTTGG+TGG 0.552948 4:+49730100 None:intergenic
TCGCTTGCCTGATGCCACCA+AGG 0.554221 4:-49730114 MsG0480020858.01.T01:CDS
ACTCACCTCGAAATCTAAGA+AGG 0.556896 4:+49731837 None:intergenic
TGGTAATCAGCACTCACGGT+TGG 0.557293 4:+49731597 None:intergenic
CGCTTGCCTGATGCCACCAA+GGG 0.557551 4:-49730113 MsG0480020858.01.T01:intron
AGTTCCTTTCCACCCCGGTA+GGG 0.567360 4:-49730775 MsG0480020858.01.T01:CDS
TCAGGCAAGCGACCCTCTGG+TGG 0.568572 4:+49730125 None:intergenic
GACATCGTTGCTCTGTCTGG+TGG 0.579861 4:-49729952 MsG0480020858.01.T01:CDS
GCAGCATATTTCTCAACAAG+TGG 0.582870 4:+49729360 None:intergenic
TTACCAAAAGGCTGTTGAGA+AGG 0.586567 4:-49731580 MsG0480020858.01.T01:CDS
TCCTTTCCACCCCGGTAGGG+AGG 0.587045 4:-49730772 MsG0480020858.01.T01:intron
TTGGTAGGGAGCTGCACACA+AGG 0.589477 4:-49729623 MsG0480020858.01.T01:intron
GTTGCTGTTGAGATTACTGG+TGG 0.592645 4:-49730804 MsG0480020858.01.T01:CDS
TAGCTATGCTGATTTCTACC+AGG 0.595859 4:-49730928 MsG0480020858.01.T01:intron
CAAGCCTGAGCCACCACCAG+AGG 0.599599 4:-49730138 MsG0480020858.01.T01:CDS
GTTCTGGATTTGAGGGCCCA+TGG 0.602005 4:-49729597 MsG0480020858.01.T01:CDS
GAAATGATTAGGGAGTTGTT+GGG 0.614317 4:-49729450 MsG0480020858.01.T01:intron
TGAGAGGATTAGAAGTCCAT+GGG 0.619251 4:+49729581 None:intergenic
AAGCCTGAGCCACCACCAGA+GGG 0.625588 4:-49730137 MsG0480020858.01.T01:CDS
CCTGAAGTTCCTTTCCACCC+CGG 0.635685 4:-49730780 MsG0480020858.01.T01:CDS
GATTCAAAGACAAAGACTGG+TGG 0.639755 4:-49731059 MsG0480020858.01.T01:CDS
TTTCTTTGCTGATTATGCCG+AGG 0.640041 4:-49729211 MsG0480020858.01.T01:CDS
CAGCATATTTCTCAACAAGT+GGG 0.640937 4:+49729361 None:intergenic
AGGCTGTTGAGAAGGCGAAG+AGG 0.646869 4:-49731572 MsG0480020858.01.T01:CDS
ATGTGTTTGGCAAAGCTATG+GGG 0.650358 4:-49729987 MsG0480020858.01.T01:CDS
AGTCAATACATACAATGGTG+TGG 0.652135 4:+49729929 None:intergenic
AAGGCGAAGAGGAAGCTCAG+AGG 0.652638 4:-49731561 MsG0480020858.01.T01:CDS
CTTGCTCATGGTGCTAACAA+CGG 0.654051 4:-49731005 MsG0480020858.01.T01:CDS
TGGTGCTTGATGGTTCCGAA+AGG 0.654886 4:+49731035 None:intergenic
TTGTTGGGTGGTGAGAAGGA+AGG 0.658316 4:-49729435 MsG0480020858.01.T01:CDS
AACGTACCTCCCTACCGGGG+TGG 0.660383 4:+49730766 None:intergenic
ATGATTAGGGAGTTGTTGGG+TGG 0.662546 4:-49729447 MsG0480020858.01.T01:intron
CATATTTCTCAACAAGTGGG+CGG 0.689114 4:+49729364 None:intergenic
GTAAACGTACCTCCCTACCG+GGG 0.769408 4:+49730763 None:intergenic
TGAACCCTAACAGTGACACG+AGG 0.770546 4:+49731877 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAAAACTCA+AGG + Chr4:49730526-49730545 None:intergenic 10.0%
!! CCAAATATAAAAAATAAATA+GGG + Chr4:49730891-49730910 None:intergenic 10.0%
!! TCCAAATATAAAAAATAAAT+AGG + Chr4:49730892-49730911 None:intergenic 10.0%
!!! ATTATCTAAGTTTTAAATTA+TGG - Chr4:49731305-49731324 MsG0480020858.01.T01:intron 10.0%
!!! ATTTAAAACACAAAAAAAAT+AGG + Chr4:49731592-49731611 None:intergenic 10.0%
!!! TATTTATTTTTTATATTTGG+AGG - Chr4:49730891-49730910 MsG0480020858.01.T01:intron 10.0%
!!! TTTAAAACACAAAAAAAATA+GGG + Chr4:49731591-49731610 None:intergenic 10.0%
!! AGGAATATTAAATATACATA+CGG + Chr4:49731753-49731772 None:intergenic 15.0%
!!! ATCTGTTTGAATATTTTTTT+GGG - Chr4:49729797-49729816 MsG0480020858.01.T01:intron 15.0%
!!! ATTATCAAAGTTTTAAACTA+TGG - Chr4:49731241-49731260 MsG0480020858.01.T01:intron 15.0%
!!! CCCTATTTATTTTTTATATT+TGG - Chr4:49730888-49730907 MsG0480020858.01.T01:intron 15.0%
!! AAGAAAAATAAGCTCAAAAA+TGG + Chr4:49729374-49729393 None:intergenic 20.0%
!! ATTTCAGTCATTTATTTATG+TGG - Chr4:49730228-49730247 MsG0480020858.01.T01:intron 20.0%
!! GATAATTAAACCAAAACAAA+AGG + Chr4:49730926-49730945 None:intergenic 20.0%
!! TATACTTAATGTAATTGGTA+GGG - Chr4:49731451-49731470 MsG0480020858.01.T01:intron 20.0%
!! TTATACTTAATGTAATTGGT+AGG - Chr4:49731450-49731469 MsG0480020858.01.T01:intron 20.0%
!! TTGGTTATACTTAATGTAAT+TGG - Chr4:49731446-49731465 MsG0480020858.01.T01:intron 20.0%
!!! AATATTTTTTTGGGATGAAT+CGG - Chr4:49729806-49729825 MsG0480020858.01.T01:intron 20.0%
!!! AATCCTTTTCATCATATTAT+TGG - Chr4:49731778-49731797 MsG0480020858.01.T01:intron 20.0%
!!! ATGATGTTTCTTTAACTTTT+TGG - Chr4:49730407-49730426 MsG0480020858.01.T01:intron 20.0%
!!! ATGATTTGACAATCAATTTT+TGG - Chr4:49730460-49730479 MsG0480020858.01.T01:intron 20.0%
!!! ATGATTTGTTTGAGATTTTT+TGG - Chr4:49729769-49729788 MsG0480020858.01.T01:intron 20.0%
!!! ATTTTCTGCTTATTTTTTGT+AGG - Chr4:49729604-49729623 MsG0480020858.01.T01:CDS 20.0%
!!! GATCTGTTTGAATATTTTTT+TGG - Chr4:49729796-49729815 MsG0480020858.01.T01:intron 20.0%
!!! TTTAAAGTCAATACATACAA+TGG + Chr4:49731167-49731186 None:intergenic 20.0%
!!! TTTGTTTTGGTTTAATTATC+AGG - Chr4:49730926-49730945 MsG0480020858.01.T01:intron 20.0%
! AAAATAAAACTTACGCCAAA+CGG + Chr4:49729589-49729608 None:intergenic 25.0%
! AAACGCATTTGCAATTATTA+CGG - Chr4:49730612-49730631 MsG0480020858.01.T01:intron 25.0%
! AATCCAAAAACTCATTGATA+AGG + Chr4:49730707-49730726 None:intergenic 25.0%
! ATGATGAAAAGGATTATCAT+AGG + Chr4:49731773-49731792 None:intergenic 25.0%
! ATGCCAATAATATGATGAAA+AGG + Chr4:49731784-49731803 None:intergenic 25.0%
! TAAATAGGGTGAAAAACAAA+AGG + Chr4:49730877-49730896 None:intergenic 25.0%
! TGATGTTGTTGAAATGATTA+GGG - Chr4:49731628-49731647 MsG0480020858.01.T01:intron 25.0%
! TTCATGTGGTATATAATGAT+AGG - Chr4:49730492-49730511 MsG0480020858.01.T01:intron 25.0%
! TTGATGTTGTTGAAATGATT+AGG - Chr4:49731627-49731646 MsG0480020858.01.T01:exon 25.0%
! TTTCTCATTGTTGATGTAAA+TGG - Chr4:49730357-49730376 MsG0480020858.01.T01:intron 25.0%
!! ATTTGATTCTTGATTTTTGC+AGG - Chr4:49731061-49731080 MsG0480020858.01.T01:CDS 25.0%
!! TTGCAGAAATTTTAAGCTAT+GGG - Chr4:49729458-49729477 MsG0480020858.01.T01:intron 25.0%
!! TTTGGTTATAACTTTTCATG+TGG - Chr4:49730478-49730497 MsG0480020858.01.T01:intron 25.0%
!! TTTTATATTTGGAGGCTAAT+TGG - Chr4:49730899-49730918 MsG0480020858.01.T01:intron 25.0%
!!! ATATTTTTTTGGGATGAATC+GGG - Chr4:49729807-49729826 MsG0480020858.01.T01:intron 25.0%
!!! ATTTATGTGGTTTGTTTTTG+TGG - Chr4:49730241-49730260 MsG0480020858.01.T01:intron 25.0%
!!! TGCATTTGATATTTTGATAC+TGG - Chr4:49731214-49731233 MsG0480020858.01.T01:intron 25.0%
!!! TTATGATTTGTTTGTGTAGA+TGG - Chr4:49729988-49730007 MsG0480020858.01.T01:CDS 25.0%
AAACAACTACAACCAATTGT+AGG + Chr4:49731033-49731052 None:intergenic 30.0%
AAATACAACAAATCACTACC+TGG + Chr4:49730181-49730200 None:intergenic 30.0%
AAATCAGCATAGCTAATGAT+TGG + Chr4:49730155-49730174 None:intergenic 30.0%
AATCAGCATAGCTAATGATT+GGG + Chr4:49730154-49730173 None:intergenic 30.0%
AATTAACCACATGTGATAAC+TGG - Chr4:49730824-49730843 MsG0480020858.01.T01:intron 30.0%
ACACAAAAAAAATAGGGCAA+TGG + Chr4:49731585-49731604 None:intergenic 30.0%
CACAAAAAAAATAGGGCAAT+GGG + Chr4:49731584-49731603 None:intergenic 30.0%
TACAATTGGTTGTAGTTGTT+TGG - Chr4:49731032-49731051 MsG0480020858.01.T01:CDS 30.0%
TATGAGTTGTCAAAAATGAG+AGG + Chr4:49731526-49731545 None:intergenic 30.0%
TCGAAATCTAAGAAGGAAAA+TGG + Chr4:49729247-49729266 None:intergenic 30.0%
TGGATTTCATACATTTCTGT+TGG - Chr4:49730721-49730740 MsG0480020858.01.T01:intron 30.0%
TGTGAATTATTGCCTACAAT+TGG - Chr4:49731018-49731037 MsG0480020858.01.T01:CDS 30.0%
TTTAAATTATGGCTGCATTG+TGG - Chr4:49731316-49731335 MsG0480020858.01.T01:intron 30.0%
TTTGATTCAAAGACAAAGAC+TGG - Chr4:49730026-49730045 MsG0480020858.01.T01:intron 30.0%
! AACTTCGATTCTGAGAAAAT+AGG + Chr4:49729316-49729335 None:intergenic 30.0%
! AGTCCTTATCAATGAGTTTT+TGG - Chr4:49730701-49730720 MsG0480020858.01.T01:intron 30.0%
! ATTTTCCTTCTTAGATTTCG+AGG - Chr4:49729246-49729265 MsG0480020858.01.T01:intron 30.0%
! GTTGCAGAAATTTTAAGCTA+TGG - Chr4:49729457-49729476 MsG0480020858.01.T01:intron 30.0%
!! GAATCAGAGAAATTTTTGAG+CGG + Chr4:49729292-49729311 None:intergenic 30.0%
!!! ATCGGGTGCAATTTTTTTTT+TGG - Chr4:49729824-49729843 MsG0480020858.01.T01:intron 30.0%
AGTCAATACATACAATGGTG+TGG + Chr4:49731162-49731181 None:intergenic 35.0%
ATCAAAAGCTATCTGAGCTT+GGG - Chr4:49731903-49731922 MsG0480020858.01.T01:five_prime_UTR 35.0%
ATTGCAAATGCGTTTCAAAC+AGG + Chr4:49730607-49730626 None:intergenic 35.0%
CAGCATATTTCTCAACAAGT+GGG + Chr4:49731730-49731749 None:intergenic 35.0%
CTACAGACAACTTTCAAAAC+AGG + Chr4:49730683-49730702 None:intergenic 35.0%
GAAATGATTAGGGAGTTGTT+GGG - Chr4:49731638-49731657 MsG0480020858.01.T01:intron 35.0%
TCTCATTTGTCAATGATTGC+AGG - Chr4:49731844-49731863 MsG0480020858.01.T01:five_prime_UTR 35.0%
TGAAATGATTAGGGAGTTGT+TGG - Chr4:49731637-49731656 MsG0480020858.01.T01:intron 35.0%
TTGCAATGCTATCTACAAGT+TGG + Chr4:49730750-49730769 None:intergenic 35.0%
TTTAAACTATGGCTGCATTG+TGG - Chr4:49731252-49731271 MsG0480020858.01.T01:intron 35.0%
!! GCATTTCTAGTTATCACATG+AGG + Chr4:49730785-49730804 None:intergenic 35.0%
!! GCTAATTGGTCCTTTTGTTT+TGG - Chr4:49730913-49730932 MsG0480020858.01.T01:intron 35.0%
!! TGTTTTTGTGGTTATGCAGT+TGG - Chr4:49730253-49730272 MsG0480020858.01.T01:intron 35.0%
AAATCCAGAACACTAACCCT+TGG + Chr4:49730994-49731013 None:intergenic 40.0%
ACTCACCTCGAAATCTAAGA+AGG + Chr4:49729254-49729273 None:intergenic 40.0%
ATGAGAGGATTAGAAGTCCA+TGG + Chr4:49731511-49731530 None:intergenic 40.0%
ATGTGTTTGGCAAAGCTATG+GGG - Chr4:49731101-49731120 MsG0480020858.01.T01:intron 40.0%
ATTGGGAATTGCTCCTTAAG+AGG + Chr4:49730137-49730156 None:intergenic 40.0%
CATATTTCTCAACAAGTGGG+CGG + Chr4:49731727-49731746 None:intergenic 40.0%
CATCAAAAGCTATCTGAGCT+TGG - Chr4:49731902-49731921 MsG0480020858.01.T01:five_prime_UTR 40.0%
GATGTGTTTGGCAAAGCTAT+GGG - Chr4:49731100-49731119 MsG0480020858.01.T01:intron 40.0%
GATTCAAAGACAAAGACTGG+TGG - Chr4:49730029-49730048 MsG0480020858.01.T01:intron 40.0%
GCAGCATATTTCTCAACAAG+TGG + Chr4:49731731-49731750 None:intergenic 40.0%
GCATTTCCAGTTATCACATG+TGG + Chr4:49730833-49730852 None:intergenic 40.0%
TATCACTTGGCAACTGAAGA+AGG + Chr4:49731679-49731698 None:intergenic 40.0%
TCTAGTTATCACATGAGGAC+AGG + Chr4:49730780-49730799 None:intergenic 40.0%
TCTTCAGTTGCCAAGTGATA+AGG - Chr4:49731679-49731698 MsG0480020858.01.T01:intron 40.0%
TGAGAGGATTAGAAGTCCAT+GGG + Chr4:49731510-49731529 None:intergenic 40.0%
TTACCAAAAGGCTGTTGAGA+AGG - Chr4:49729508-49729527 MsG0480020858.01.T01:intron 40.0%
! AGATAGCTTTTGATGAGCCT+CGG + Chr4:49731897-49731916 None:intergenic 40.0%
! CGTTGAGATTGTGACTGTTT+TGG - Chr4:49731427-49731446 MsG0480020858.01.T01:intron 40.0%
! GTTGTTGCTGTTGAGATTAC+TGG - Chr4:49730281-49730300 MsG0480020858.01.T01:intron 40.0%
! TGATATCGCTGTTAGGCTTT+TGG - Chr4:49730109-49730128 MsG0480020858.01.T01:intron 40.0%
! TTTCTTTGCTGATTATGCCG+AGG - Chr4:49731877-49731896 MsG0480020858.01.T01:five_prime_UTR 40.0%
!! CTCAAAAGTGCCTTATCACT+TGG + Chr4:49731692-49731711 None:intergenic 40.0%
!! CTTTTGGTAATCAGCACTCA+CGG + Chr4:49729498-49729517 None:intergenic 40.0%
!! GGAGGTACGTTTACTACTTT+TGG - Chr4:49730334-49730353 MsG0480020858.01.T01:intron 40.0%
!! TAGCTATGCTGATTTCTACC+AGG - Chr4:49730160-49730179 MsG0480020858.01.T01:intron 40.0%
ACAAAGACTGGTGGTCCTTT+CGG - Chr4:49730038-49730057 MsG0480020858.01.T01:intron 45.0%
ACGGTCTTGATATCGCTGTT+AGG - Chr4:49730102-49730121 MsG0480020858.01.T01:intron 45.0%
ATGATTAGGGAGTTGTTGGG+TGG - Chr4:49731641-49731660 MsG0480020858.01.T01:intron 45.0%
GACCATTTGAGGGATGTGTT+TGG - Chr4:49731088-49731107 MsG0480020858.01.T01:intron 45.0%
GGATGTGTTTGGCAAAGCTA+TGG - Chr4:49731099-49731118 MsG0480020858.01.T01:intron 45.0%
TAGTAAACGTACCTCCCTAC+CGG + Chr4:49730330-49730349 None:intergenic 45.0%
TGCCAAACACATCCCTCAAA+TGG + Chr4:49731093-49731112 None:intergenic 45.0%
TGTCTTGCAGTTCACGTTCT+TGG - Chr4:49730643-49730662 MsG0480020858.01.T01:intron 45.0%
TTTGTGGTTATGCAGTTGGC+TGG - Chr4:49730257-49730276 MsG0480020858.01.T01:intron 45.0%
! CTTGCTCATGGTGCTAACAA+CGG - Chr4:49730083-49730102 MsG0480020858.01.T01:intron 45.0%
! GTTGCTGTTGAGATTACTGG+TGG - Chr4:49730284-49730303 MsG0480020858.01.T01:intron 45.0%
! TGCAGGTTCTGACCATTTGA+GGG - Chr4:49731078-49731097 MsG0480020858.01.T01:CDS 45.0%
! TTGCAGGTTCTGACCATTTG+AGG - Chr4:49731077-49731096 MsG0480020858.01.T01:CDS 45.0%
!! AAGGAGCGTTCTGGATTTGA+GGG - Chr4:49731484-49731503 MsG0480020858.01.T01:intron 45.0%
!! CGTGAGTGCTGATTACCAAA+AGG - Chr4:49729496-49729515 MsG0480020858.01.T01:intron 45.0%
!!! TAGGCTTTTGGAGCCTCTTA+AGG - Chr4:49730121-49730140 MsG0480020858.01.T01:CDS 45.0%
AGTAAACGTACCTCCCTACC+GGG + Chr4:49730329-49730348 None:intergenic 50.0%
CAACAAGTGGGCGGAATACA+GGG + Chr4:49731718-49731737 None:intergenic 50.0%
GGTAATCAGCACTCACGGTT+GGG + Chr4:49729493-49729512 None:intergenic 50.0%
TACGCCAAACGGAGCATAAG+AGG + Chr4:49729578-49729597 None:intergenic 50.0%
TCAACAAGTGGGCGGAATAC+AGG + Chr4:49731719-49731738 None:intergenic 50.0%
TCCAGAACACTAACCCTTGG+TGG + Chr4:49730991-49731010 None:intergenic 50.0%
TGAACCCTAACAGTGACACG+AGG + Chr4:49729214-49729233 None:intergenic 50.0%
TGGTAATCAGCACTCACGGT+TGG + Chr4:49729494-49729513 None:intergenic 50.0%
TTGTGTAGATGGCACTCTGC+TGG - Chr4:49729999-49730018 MsG0480020858.01.T01:CDS 50.0%
TTGTTGGGTGGTGAGAAGGA+AGG - Chr4:49731653-49731672 MsG0480020858.01.T01:intron 50.0%
! CTCTCCTCGTGTCACTGTTA+GGG - Chr4:49729207-49729226 MsG0480020858.01.T01:CDS 50.0%
! TCGCCTTCTCAACAGCCTTT+TGG + Chr4:49729514-49729533 None:intergenic 50.0%
! TGGTGCTTGATGGTTCCGAA+AGG + Chr4:49730056-49730075 None:intergenic 50.0%
!! AAGCTCAGCTTGGTGCTTGA+TGG + Chr4:49730066-49730085 None:intergenic 50.0%
!! CAAGGAGCGTTCTGGATTTG+AGG - Chr4:49731483-49731502 MsG0480020858.01.T01:intron 50.0%
AAGGCGAAGAGGAAGCTCAG+AGG - Chr4:49729527-49729546 MsG0480020858.01.T01:intron 55.0%
AAGTTCCTTTCCACCCCGGT+AGG - Chr4:49730312-49730331 MsG0480020858.01.T01:intron 55.0%
AGCTATGGGGCTTAGTGACC+AGG - Chr4:49731114-49731133 MsG0480020858.01.T01:intron 55.0%
AGGCTGTTGAGAAGGCGAAG+AGG - Chr4:49729516-49729535 MsG0480020858.01.T01:intron 55.0%
AGTTCCTTTCCACCCCGGTA+GGG - Chr4:49730313-49730332 MsG0480020858.01.T01:intron 55.0%
CACCATGAGCAAGCTCAGCT+TGG + Chr4:49730076-49730095 None:intergenic 55.0%
CAGACAGAGCAACGATGTCC+TGG + Chr4:49731135-49731154 None:intergenic 55.0%
CAGGACATCGTTGCTCTGTC+TGG - Chr4:49731133-49731152 MsG0480020858.01.T01:intron 55.0%
CCTGAAGTTCCTTTCCACCC+CGG - Chr4:49730308-49730327 MsG0480020858.01.T01:intron 55.0%
CGCTCCTCTTATGCTCCGTT+TGG - Chr4:49729571-49729590 MsG0480020858.01.T01:CDS 55.0%
GACATCGTTGCTCTGTCTGG+TGG - Chr4:49731136-49731155 MsG0480020858.01.T01:intron 55.0%
GGAGTTGTTGGGTGGTGAGA+AGG - Chr4:49731649-49731668 MsG0480020858.01.T01:intron 55.0%
GTAAACGTACCTCCCTACCG+GGG + Chr4:49730328-49730347 None:intergenic 55.0%
TTGGTAGGGAGCTGCACACA+AGG - Chr4:49731465-49731484 MsG0480020858.01.T01:intron 55.0%
! CACCAAGCTGAGCTTGCTCA+TGG - Chr4:49730071-49730090 MsG0480020858.01.T01:intron 55.0%
! CACTAACCCTTGGTGGCATC+AGG + Chr4:49730984-49731003 None:intergenic 55.0%
! GCCACCAAGGGTTAGTGTTC+TGG - Chr4:49730987-49731006 MsG0480020858.01.T01:CDS 55.0%
! GCTCTCCTCGTGTCACTGTT+AGG - Chr4:49729206-49729225 MsG0480020858.01.T01:CDS 55.0%
! GTTCTGGATTTGAGGGCCCA+TGG - Chr4:49731491-49731510 MsG0480020858.01.T01:intron 55.0%
AAGCCTGAGCCACCACCAGA+GGG - Chr4:49730951-49730970 MsG0480020858.01.T01:CDS 60.0%
CCGGGGTGGAAAGGAACTTC+AGG + Chr4:49730311-49730330 None:intergenic 60.0%
CGCTTGCCTGATGCCACCAA+GGG - Chr4:49730975-49730994 MsG0480020858.01.T01:CDS 60.0%
TCGCTTGCCTGATGCCACCA+AGG - Chr4:49730974-49730993 MsG0480020858.01.T01:CDS 60.0%
!! GCTGCACACAAGGAGCGTTC+TGG - Chr4:49731475-49731494 MsG0480020858.01.T01:intron 60.0%
AACGTACCTCCCTACCGGGG+TGG + Chr4:49730325-49730344 None:intergenic 65.0%
ACCTCCCTACCGGGGTGGAA+AGG + Chr4:49730320-49730339 None:intergenic 65.0%
CAAGCCTGAGCCACCACCAG+AGG - Chr4:49730950-49730969 MsG0480020858.01.T01:CDS 65.0%
GCATCAGGCAAGCGACCCTC+TGG + Chr4:49730969-49730988 None:intergenic 65.0%
TCAGGCAAGCGACCCTCTGG+TGG + Chr4:49730966-49730985 None:intergenic 65.0%
TCCTTTCCACCCCGGTAGGG+AGG - Chr4:49730316-49730335 MsG0480020858.01.T01:intron 65.0%
GGCAAGCGACCCTCTGGTGG+TGG + Chr4:49730963-49730982 None:intergenic 70.0%
!! CGACCCTCTGGTGGTGGCTC+AGG + Chr4:49730957-49730976 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 49729182 49731928 49729182 ID=MsG0480020858.01;Name=MsG0480020858.01
Chr4 mRNA 49729182 49731928 49729182 ID=MsG0480020858.01.T01;Parent=MsG0480020858.01;Name=MsG0480020858.01.T01;_AED=0.38;_eAED=0.38;_QI=98|1|0.88|1|0.87|0.77|9|0|246
Chr4 exon 49729182 49729244 49729182 ID=MsG0480020858.01.T01:exon:16564;Parent=MsG0480020858.01.T01
Chr4 exon 49729359 49729461 49729359 ID=MsG0480020858.01.T01:exon:16563;Parent=MsG0480020858.01.T01
Chr4 exon 49729559 49729638 49729559 ID=MsG0480020858.01.T01:exon:16562;Parent=MsG0480020858.01.T01
Chr4 exon 49729942 49730027 49729942 ID=MsG0480020858.01.T01:exon:16561;Parent=MsG0480020858.01.T01
Chr4 exon 49730114 49730162 49730114 ID=MsG0480020858.01.T01:exon:16560;Parent=MsG0480020858.01.T01
Chr4 exon 49730773 49730838 49730773 ID=MsG0480020858.01.T01:exon:16559;Parent=MsG0480020858.01.T01
Chr4 exon 49730929 49731103 49730929 ID=MsG0480020858.01.T01:exon:16558;Parent=MsG0480020858.01.T01
Chr4 exon 49731516 49731646 49731516 ID=MsG0480020858.01.T01:exon:16557;Parent=MsG0480020858.01.T01
Chr4 exon 49731843 49731928 49731843 ID=MsG0480020858.01.T01:exon:16556;Parent=MsG0480020858.01.T01
Chr4 five_prime_UTR 49731843 49731928 49731843 ID=MsG0480020858.01.T01:five_prime_utr;Parent=MsG0480020858.01.T01
Chr4 five_prime_UTR 49731635 49731646 49731635 ID=MsG0480020858.01.T01:five_prime_utr;Parent=MsG0480020858.01.T01
Chr4 CDS 49731516 49731634 49731516 ID=MsG0480020858.01.T01:cds;Parent=MsG0480020858.01.T01
Chr4 CDS 49730929 49731103 49730929 ID=MsG0480020858.01.T01:cds;Parent=MsG0480020858.01.T01
Chr4 CDS 49730773 49730838 49730773 ID=MsG0480020858.01.T01:cds;Parent=MsG0480020858.01.T01
Chr4 CDS 49730114 49730162 49730114 ID=MsG0480020858.01.T01:cds;Parent=MsG0480020858.01.T01
Chr4 CDS 49729942 49730027 49729942 ID=MsG0480020858.01.T01:cds;Parent=MsG0480020858.01.T01
Chr4 CDS 49729559 49729638 49729559 ID=MsG0480020858.01.T01:cds;Parent=MsG0480020858.01.T01
Chr4 CDS 49729359 49729461 49729359 ID=MsG0480020858.01.T01:cds;Parent=MsG0480020858.01.T01
Chr4 CDS 49729182 49729244 49729182 ID=MsG0480020858.01.T01:cds;Parent=MsG0480020858.01.T01
Gene Sequence

>MsG0480020858.01.T01

ATGGGAAAATCTTACCCAACCGTGAGTGCTGATTACCAAAAGGCTGTTGAGAAGGCGAAGAGGAAGCTCAGAGGTTTCATCGCTGAGAAGAAATGCGCTCCTCTTATGCTCCGTTTGGCATGGCACTCTGCTGGAACTTTTGATTCAAAGACAAAGACTGGTGGTCCTTTCGGAACCATCAAGCACCAAGCTGAGCTTGCTCATGGTGCTAACAACGGTCTTGATATCGCTGTTAGGCTTTTGGAGCCTCTTAAGGAGCAATTCCCAATCATTAGCTATGCTGATTTCTACCAGTTGGCTGGTGTTGTTGCTGTTGAGATTACTGGTGGACCTGAAGTTCCTTTCCACCCCGGTAGGGAGGACAAGCCTGAGCCACCACCAGAGGGTCGCTTGCCTGATGCCACCAAGGGTTCTGACCATTTGAGGGATGTGTTTGGCAAAGCTATGGGGCTTAGTGACCAGGACATCGTTGCTCTGTCTGGTGGCCACACCATTGGAGCTGCACACAAGGAGCGTTCTGGATTTGAGGGCCCATGGACTTCTAATCCTCTCATTTTTGACAACTCATACTTCAAGGAGTTGTTGGGTGGTGAGAAGGAAGGCCTTCTTCAGTTGCCAAGTGATAAGGCACTTTTGAGCGACCCTGTATTCCGCCCACTTGTTGAGAAATATGCTGCCGATGAAGATGCTTTCTTTGCTGATTATGCCGAGGCTCATCAAAAGCTATCTGAGCTTGGGTAA
Protein sequence

>MsG0480020858.01.T01

MGKSYPTVSADYQKAVEKAKRKLRGFIAEKKCAPLMLRLAWHSAGTFDSKTKTGGPFGTIKHQAELAHGANNGLDIAVRLLEPLKEQFPIISYADFYQLAGVVAVEITGGPEVPFHPGREDKPEPPPEGRLPDATKGSDHLRDVFGKAMGLSDQDIVALSGGHTIGAAHKERSGFEGPWTSNPLIFDNSYFKELLGGEKEGLLQLPSDKALLSDPVFRPLVEKYAADEDAFFADYAEAHQKLSELG*