AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021183.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021183.01.T01 MTR_5g022580 89.529 191 20 0 1 191 158 348 1.28e-121 351
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021183.01.T01 AT5G66120 79.581 191 39 0 1 191 53 243 3.96e-108 313
MsG0480021183.01.T01 AT5G66120 79.581 191 39 0 1 191 157 347 1.59e-107 315

Find 110 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACTGTTTATTATATTATT+TGG 0.156689 4:+54822433 MsG0480021183.01.T01:CDS
TGGAATTGGAATTGGAATTT+TGG 0.217615 4:-54819743 None:intergenic
CAACCCTGATCCGATATAAA+TGG 0.217921 4:-54820034 None:intergenic
TTTGCAGAAGTTATAAAGTA+TGG 0.237109 4:+54821678 MsG0480021183.01.T01:CDS
AGTCAGGGCTACATTGAATT+TGG 0.243315 4:+54822383 MsG0480021183.01.T01:CDS
CACCATTGTTAACGTTGATT+TGG 0.244416 4:+54819996 MsG0480021183.01.T01:five_prime_UTR
TTATAAAGTATGGGCTCATT+AGG 0.291558 4:+54821688 MsG0480021183.01.T01:CDS
TGAAGGGAGGAGAGATGAAT+TGG 0.309849 4:-54819817 None:intergenic
TTGAATTTGGGTCATACATT+TGG 0.323104 4:+54822396 MsG0480021183.01.T01:CDS
CCCTTGGAGGTGGGTGTGAT+TGG 0.327402 4:+54821297 MsG0480021183.01.T01:CDS
ATAGCATATGCCAGTGCATT+AGG 0.327626 4:-54822303 None:intergenic
TCTTCAGTTGGTGGCAAAAC+TGG 0.330298 4:+54821543 MsG0480021183.01.T01:CDS
TCTCTCTTTCTACACCTCTT+AGG 0.330717 4:+54819905 MsG0480021183.01.T01:five_prime_UTR
ACACTCTCAACAGAAACATT+TGG 0.341474 4:-54821087 None:intergenic
CACTCTCAACAGAAACATTT+GGG 0.343857 4:-54821086 None:intergenic
TGGGGTGAGGGGAGAAAGAG+TGG 0.345133 4:-54819861 None:intergenic
ACCATTGTTAACGTTGATTT+GGG 0.350396 4:+54819997 MsG0480021183.01.T01:five_prime_UTR
TCAGATTCCAACCACTGTTA+TGG 0.372653 4:+54821370 MsG0480021183.01.T01:CDS
ATGAGAGAGAAGGTGGAGTC+AGG 0.374462 4:+54822367 MsG0480021183.01.T01:CDS
TTAACGTTGATTTGGGTAAT+CGG 0.380324 4:+54820004 MsG0480021183.01.T01:five_prime_UTR
CAAGGCCGAGGTTGTGTCCT+TGG 0.381716 4:+54822344 MsG0480021183.01.T01:CDS
GAAGCCATTGGAATTGGAAT+TGG 0.383764 4:-54819751 None:intergenic
TATCCCATTTATATCGGATC+AGG 0.386598 4:+54820030 MsG0480021183.01.T01:five_prime_UTR
GGGTGAGGGGAGAAAGAGTG+GGG 0.392595 4:-54819859 None:intergenic
CCAGAGAGGGAACTGGCATC+AGG 0.397934 4:+54821654 MsG0480021183.01.T01:CDS
GTCAGGGCTACATTGAATTT+GGG 0.399767 4:+54822384 MsG0480021183.01.T01:CDS
CGGAGCTATCCCATTTATAT+CGG 0.402539 4:+54820024 MsG0480021183.01.T01:five_prime_UTR
GGTGTGGAAGCCATTGGAAT+TGG 0.406933 4:-54819757 None:intergenic
GAAGGAGAATTGGGTGGGTG+TGG 0.418520 4:-54819773 None:intergenic
CCGTAGTGTTAGTGACAATA+AGG 0.430212 4:-54821015 None:intergenic
CCCCTTGGGAAGAACTTGAT+TGG 0.431232 4:+54821576 MsG0480021183.01.T01:CDS
AATTACCTTTGAAGAAGTTC+AGG 0.444038 4:-54820066 None:intergenic
CCTGATGCCAGTTCCCTCTC+TGG 0.447867 4:-54821654 None:intergenic
TATAAAGTATGGGCTCATTA+GGG 0.460868 4:+54821689 MsG0480021183.01.T01:CDS
TGAGAGGAACTAGCGCATAT+TGG 0.463868 4:-54819931 None:intergenic
AACCCTGATCCGATATAAAT+GGG 0.471338 4:-54820033 None:intergenic
CGCTAGTTCCTCTCAACTCA+TGG 0.474787 4:+54819939 MsG0480021183.01.T01:five_prime_UTR
TTCATACATCAAGAAAGAAA+AGG 0.479772 4:-54822482 None:intergenic
TCCTTGTACTGCTCACCATC+CGG 0.486431 4:-54821117 None:intergenic
TGAGAGAGAAGGTGGAGTCA+GGG 0.489001 4:+54822368 MsG0480021183.01.T01:CDS
AAAATGGTTGGTGGGGTGAG+GGG 0.495621 4:-54819872 None:intergenic
TAGGTTGATTCTTCAGTTGG+TGG 0.498341 4:+54821534 MsG0480021183.01.T01:intron
CAAGGCCATTGAGTCTCGAT+TGG 0.499244 4:+54821253 MsG0480021183.01.T01:CDS
GTTGTTGATGCTTTAACAAG+TGG 0.499540 4:+54821060 MsG0480021183.01.T01:five_prime_UTR
AAATCAACGTTAACAATGGT+GGG 0.500362 4:-54819994 None:intergenic
GGGGTGAGGGGAGAAAGAGT+GGG 0.502422 4:-54819860 None:intergenic
CAATAAGGACCTTCTTTCCA+TGG 0.505079 4:-54821000 None:intergenic
TGGTTGTTGTTTGACGGAGG+GGG 0.506790 4:-54819797 None:intergenic
ATATGCAGGCATTAATGGCC+AGG 0.511276 4:+54821742 MsG0480021183.01.T01:CDS
TTGTAGGTTGATTCTTCAGT+TGG 0.512666 4:+54821531 MsG0480021183.01.T01:intron
CCGATCCAATCGAGACTCAA+TGG 0.515263 4:-54821258 None:intergenic
TTGCAGAAGTTATAAAGTAT+GGG 0.520923 4:+54821679 MsG0480021183.01.T01:CDS
AATTCCAATTCCAATTCCAA+TGG 0.522192 4:+54819747 MsG0480021183.01.T01:five_prime_UTR
ATGAATTGGTTGTTGTTTGA+CGG 0.522694 4:-54819803 None:intergenic
CTGGTATAAACCATCCCCTT+GGG 0.525333 4:+54821562 MsG0480021183.01.T01:CDS
AATTGGTTGTTGTTTGACGG+AGG 0.527859 4:-54819800 None:intergenic
TACATTACCAGAGAGGGAAC+TGG 0.528745 4:+54821647 MsG0480021183.01.T01:CDS
TTAACAATGGTGGGGACACC+AGG 0.531488 4:-54819985 None:intergenic
ACCTGTGCCATAACAGTGGT+TGG 0.531757 4:-54821377 None:intergenic
ACCGGATGGTGAGCAGTACA+AGG 0.533496 4:+54821116 MsG0480021183.01.T01:five_prime_UTR
CAAATCAACGTTAACAATGG+TGG 0.540010 4:-54819995 None:intergenic
TCAAGTTCTTCCCAAGGGGA+TGG 0.545666 4:-54821572 None:intergenic
TAGGCATGTCCATGGAAAGA+AGG 0.549371 4:+54820991 MsG0480021183.01.T01:intron
TTGGTTGTTGTTTGACGGAG+GGG 0.555042 4:-54819798 None:intergenic
TATAATAATAGGCATGTCCA+TGG 0.555537 4:+54820983 MsG0480021183.01.T01:intron
TTGCAGAGATCCTAATGCAC+TGG 0.562804 4:+54822293 MsG0480021183.01.T01:intron
ACTGGTATAAACCATCCCCT+TGG 0.563182 4:+54821561 MsG0480021183.01.T01:CDS
ATAAACCTGAACTTCTTCAA+AGG 0.564077 4:+54820061 MsG0480021183.01.T01:five_prime_UTR
GCCAATCACACCCACCTCCA+AGG 0.564253 4:-54821298 None:intergenic
AACAACCTTGTCTAGATACA+AGG 0.565489 4:-54821043 None:intergenic
TCCAACCACTGTTATGGCAC+AGG 0.567423 4:+54821376 MsG0480021183.01.T01:CDS
CAATGGTGGGGACACCAGGT+TGG 0.572157 4:-54819981 None:intergenic
ACCAATCAAGTTCTTCCCAA+GGG 0.573622 4:-54821577 None:intergenic
CACCAATCAAGTTCTTCCCA+AGG 0.573892 4:-54821578 None:intergenic
ATTGGTTGTTGTTTGACGGA+GGG 0.580028 4:-54819799 None:intergenic
CAGAGAGGGAACTGGCATCA+GGG 0.581095 4:+54821655 MsG0480021183.01.T01:CDS
CCAATCACACCCACCTCCAA+GGG 0.581252 4:-54821297 None:intergenic
TGGGTGGGTGTGGAAGCCAT+TGG 0.582947 4:-54819763 None:intergenic
ATCCCATTTATATCGGATCA+GGG 0.585337 4:+54820031 MsG0480021183.01.T01:five_prime_UTR
CTATAAGCACACATTGAGGT+TGG 0.590057 4:-54821608 None:intergenic
GCGCATATTGGTGACCTAAG+AGG 0.590433 4:-54819919 None:intergenic
GGTGAGGGGAGAAAGAGTGG+GGG 0.590686 4:-54819858 None:intergenic
CCTTATTGTCACTAACACTA+CGG 0.592911 4:+54821015 MsG0480021183.01.T01:five_prime_UTR
TCCACCTTCTCTCTCATCCA+AGG 0.607242 4:-54822361 None:intergenic
GGGGAGAAGGAGAATTGGGT+GGG 0.611458 4:-54819778 None:intergenic
ATTAGATACATTACCAGAGA+GGG 0.613639 4:+54821641 MsG0480021183.01.T01:CDS
ATCATGTGAAAACAAGGCCG+AGG 0.615090 4:+54822332 MsG0480021183.01.T01:CDS
TTGAGTCTCGATTGGATCGG+CGG 0.622236 4:+54821261 MsG0480021183.01.T01:CDS
ACCCAAATCAACGTTAACAA+TGG 0.628458 4:-54819998 None:intergenic
CTCATCCAAGGACACAACCT+CGG 0.631144 4:-54822349 None:intergenic
AATCAACGTTAACAATGGTG+GGG 0.636830 4:-54819993 None:intergenic
ATGTAACAAGAATATCAACA+TGG 0.639429 4:-54819703 None:intergenic
AAAGCGATCATGTGAAAACA+AGG 0.643424 4:+54822326 MsG0480021183.01.T01:CDS
AAAGGGGTCCATGAGTTGAG+AGG 0.643620 4:-54819947 None:intergenic
TCCTTGGATGAGAGAGAAGG+TGG 0.653001 4:+54822360 MsG0480021183.01.T01:CDS
AACACCACGTAGGAATGAAG+CGG 0.653409 4:-54821339 None:intergenic
TTGCACCTTGTATCTAGACA+AGG 0.658340 4:+54821038 MsG0480021183.01.T01:five_prime_UTR
TGGTGAGCAGTACAAGGACA+TGG 0.659373 4:+54821122 MsG0480021183.01.T01:exon
AAACAGTAAAACTACTCACA+TGG 0.660422 4:-54822419 None:intergenic
TGTAACAAGAATATCAACAT+GGG 0.661041 4:-54819702 None:intergenic
CCATTGAGTCTCGATTGGAT+CGG 0.669888 4:+54821258 MsG0480021183.01.T01:CDS
GTGTCCTTGGATGAGAGAGA+AGG 0.674887 4:+54822357 MsG0480021183.01.T01:CDS
CCAATCAAGTTCTTCCCAAG+GGG 0.683553 4:-54821576 None:intergenic
TAAGACCTGTGCCATAACAG+TGG 0.688059 4:-54821381 None:intergenic
GTGTCTATAAGCACACATTG+AGG 0.695429 4:-54821612 None:intergenic
TCTTATGAAAGTGTTTGACA+AGG 0.696371 4:+54821235 MsG0480021183.01.T01:CDS
GGTGTGATTGGCGACATGTG+TGG 0.702803 4:+54821309 MsG0480021183.01.T01:CDS
CATTAGATACATTACCAGAG+AGG 0.702812 4:+54821640 MsG0480021183.01.T01:CDS
GAATAAAATTAACACCACGT+AGG 0.705762 4:-54821349 None:intergenic
GCTGCCGCTTCATTCCTACG+TGG 0.758048 4:+54821335 MsG0480021183.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTCATGTTTATAATAAT+AGG + Chr4:54820972-54820991 MsG0480021183.01.T01:intron 10.0%
!! TTAAAAGAAAATTAAAATAG+GGG + Chr4:54821987-54822006 MsG0480021183.01.T01:intron 10.0%
!! TTACTGTTTATTATATTATT+TGG + Chr4:54822433-54822452 MsG0480021183.01.T01:CDS 10.0%
!!! AATATTAAATTTTAGACAAA+TGG + Chr4:54820466-54820485 MsG0480021183.01.T01:intron 10.0%
!! AATACAAAATTCAATGAATA+TGG - Chr4:54821507-54821526 None:intergenic 15.0%
!!! GATATTATTGAAGATATTTT+AGG + Chr4:54820262-54820281 MsG0480021183.01.T01:intron 15.0%
!!! TTTTTTTTTTTTTGAAGTTG+TGG + Chr4:54820710-54820729 MsG0480021183.01.T01:intron 15.0%
!! AAACTATGATGATTATGAAA+AGG - Chr4:54820690-54820709 None:intergenic 20.0%
!! AAATATGTAACATGATGATT+AGG + Chr4:54820735-54820754 MsG0480021183.01.T01:intron 20.0%
!! AAATTCAATGAATATGGAAA+AGG - Chr4:54821501-54821520 None:intergenic 20.0%
!! AATATGTAACATGATGATTA+GGG + Chr4:54820736-54820755 MsG0480021183.01.T01:intron 20.0%
!! AATCACATAATTAAGAAAGT+TGG + Chr4:54820439-54820458 MsG0480021183.01.T01:intron 20.0%
!! TATCTTCAATAATATCTACA+TGG - Chr4:54820258-54820277 None:intergenic 20.0%
!!! GAATTTTGTATTATGTTTGT+AGG + Chr4:54821515-54821534 MsG0480021183.01.T01:intron 20.0%
!!! GAGAGATTTTTTTAGAAAAA+TGG - Chr4:54819891-54819910 None:intergenic 20.0%
!!! GATTTAAGTTGTATACTTTT+AGG + Chr4:54820211-54820230 MsG0480021183.01.T01:intron 20.0%
!!! GATTTTTTTAGAAAAATGGT+TGG - Chr4:54819887-54819906 None:intergenic 20.0%
!!! TTCCAACTTTTACAAATTAT+CGG + Chr4:54822264-54822283 MsG0480021183.01.T01:intron 20.0%
! AGATAGACAAACAAGAAATA+CGG - Chr4:54822197-54822216 None:intergenic 25.0%
! ATATGTAACATGATGATTAG+GGG + Chr4:54820737-54820756 MsG0480021183.01.T01:intron 25.0%
! ATGTAACAAGAATATCAACA+TGG - Chr4:54819706-54819725 None:intergenic 25.0%
! CAAACAAGGTTCTATATTTA+GGG - Chr4:54820415-54820434 None:intergenic 25.0%
! CTATACTAATCATCAAACAT+AGG - Chr4:54820144-54820163 None:intergenic 25.0%
! GTATTATACAATTTACGTAG+TGG + Chr4:54820864-54820883 MsG0480021183.01.T01:intron 25.0%
! TCAAACAAGGTTCTATATTT+AGG - Chr4:54820416-54820435 None:intergenic 25.0%
! TGTAACAAGAATATCAACAT+GGG - Chr4:54819705-54819724 None:intergenic 25.0%
! TTAACAAGTTTCAAGAGAAT+TGG + Chr4:54820495-54820514 MsG0480021183.01.T01:intron 25.0%
! TTACACTGTACATATTACTT+GGG + Chr4:54821836-54821855 MsG0480021183.01.T01:intron 25.0%
! TTATGTGATTTAGTCAAACA+AGG - Chr4:54820429-54820448 None:intergenic 25.0%
! TTGCAGAAGTTATAAAGTAT+GGG + Chr4:54821679-54821698 MsG0480021183.01.T01:CDS 25.0%
! TTTGCAGAAGTTATAAAGTA+TGG + Chr4:54821678-54821697 MsG0480021183.01.T01:CDS 25.0%
!! AGAGTAATAGAGCATTATAA+AGG - Chr4:54822245-54822264 None:intergenic 25.0%
!! TACGAAAATGGTATAGTTTT+AGG - Chr4:54822144-54822163 None:intergenic 25.0%
!! TAGAAAGTTAGGATTTTGAA+GGG - Chr4:54819836-54819855 None:intergenic 25.0%
!!! CACGTTCTCAATTTTAATTA+AGG + Chr4:54820189-54820208 MsG0480021183.01.T01:intron 25.0%
!!! CTTTTAAAAAACTGTAGTAG+TGG - Chr4:54821974-54821993 None:intergenic 25.0%
!!! TTTTGTGTTATTTACACTCT+TGG + Chr4:54821171-54821190 MsG0480021183.01.T01:intron 25.0%
!!! TTTTTAGAAAAATGGTTGGT+GGG - Chr4:54819883-54819902 None:intergenic 25.0%
!!! TTTTTTAGAAAAATGGTTGG+TGG - Chr4:54819884-54819903 None:intergenic 25.0%
AAACAGTAAAACTACTCACA+TGG - Chr4:54822422-54822441 None:intergenic 30.0%
AAATCAACGTTAACAATGGT+GGG - Chr4:54819997-54820016 None:intergenic 30.0%
AATTCCAATTCCAATTCCAA+TGG + Chr4:54819747-54819766 MsG0480021183.01.T01:five_prime_UTR 30.0%
AGTAGGACACTATACAATTA+GGG - Chr4:54820903-54820922 None:intergenic 30.0%
ATAAACCTGAACTTCTTCAA+AGG + Chr4:54820061-54820080 MsG0480021183.01.T01:five_prime_UTR 30.0%
ATGTACAGATACTACGAAAA+TGG - Chr4:54822156-54822175 None:intergenic 30.0%
ATTAGATACATTACCAGAGA+GGG + Chr4:54821641-54821660 MsG0480021183.01.T01:CDS 30.0%
CATGTAATGTGAAGTCAAAA+AGG - Chr4:54821900-54821919 None:intergenic 30.0%
GAAAAATATGCAGGCATTAA+TGG + Chr4:54821737-54821756 MsG0480021183.01.T01:CDS 30.0%
GAATAAAATTAACACCACGT+AGG - Chr4:54821352-54821371 None:intergenic 30.0%
GTTACACTGTACATATTACT+TGG + Chr4:54821835-54821854 MsG0480021183.01.T01:intron 30.0%
TATAAAGTATGGGCTCATTA+GGG + Chr4:54821689-54821708 MsG0480021183.01.T01:CDS 30.0%
TATAATAATAGGCATGTCCA+TGG + Chr4:54820983-54821002 MsG0480021183.01.T01:intron 30.0%
TCTACATGGTGCAATTTAAT+TGG - Chr4:54820244-54820263 None:intergenic 30.0%
TTATAAAGTATGGGCTCATT+AGG + Chr4:54821688-54821707 MsG0480021183.01.T01:CDS 30.0%
TTGAATTTGGGTCATACATT+TGG + Chr4:54822396-54822415 MsG0480021183.01.T01:CDS 30.0%
! AAATTGCTACTTAAAGCGAA+AGG - Chr4:54820667-54820686 None:intergenic 30.0%
! AATTACCTTTGAAGAAGTTC+AGG - Chr4:54820069-54820088 None:intergenic 30.0%
! AATTGGAATTGGAATTTTGG+TGG - Chr4:54819743-54819762 None:intergenic 30.0%
! ACTCAAAGTAAGATTTTACC+TGG - Chr4:54821763-54821782 None:intergenic 30.0%
! GTAGAAAGTTAGGATTTTGA+AGG - Chr4:54819837-54819856 None:intergenic 30.0%
! TAGAGCATTATAAAGGCAAT+TGG - Chr4:54822238-54822257 None:intergenic 30.0%
! TGCCGATAATTTGTAAAAGT+TGG - Chr4:54822269-54822288 None:intergenic 30.0%
! TGGAATTGGAATTGGAATTT+TGG - Chr4:54819746-54819765 None:intergenic 30.0%
! TTACTTGGGAAATGTACTTT+TGG + Chr4:54821850-54821869 MsG0480021183.01.T01:intron 30.0%
!! ACCATTGTTAACGTTGATTT+GGG + Chr4:54819997-54820016 MsG0480021183.01.T01:five_prime_UTR 30.0%
!! ATGAATTGGTTGTTGTTTGA+CGG - Chr4:54819806-54819825 None:intergenic 30.0%
!! TCTTATGAAAGTGTTTGACA+AGG + Chr4:54821235-54821254 MsG0480021183.01.T01:CDS 30.0%
!! TCTTTTTGTTTAACTGAACG+TGG - Chr4:54819675-54819694 None:intergenic 30.0%
!! TGTTGAGAGTGTAATTTTAC+CGG + Chr4:54821098-54821117 MsG0480021183.01.T01:five_prime_UTR 30.0%
!! TTAACGTTGATTTGGGTAAT+CGG + Chr4:54820004-54820023 MsG0480021183.01.T01:five_prime_UTR 30.0%
!! TTTTAGAAAAATGGTTGGTG+GGG - Chr4:54819882-54819901 None:intergenic 30.0%
AAAGCGATCATGTGAAAACA+AGG + Chr4:54822326-54822345 MsG0480021183.01.T01:CDS 35.0%
AACAACCTTGTCTAGATACA+AGG - Chr4:54821046-54821065 None:intergenic 35.0%
AACCCTGATCCGATATAAAT+GGG - Chr4:54820036-54820055 None:intergenic 35.0%
AATCAACGTTAACAATGGTG+GGG - Chr4:54819996-54820015 None:intergenic 35.0%
ACACTCTCAACAGAAACATT+TGG - Chr4:54821090-54821109 None:intergenic 35.0%
ACATGGAAGAAGCTAGTAAT+AGG - Chr4:54821813-54821832 None:intergenic 35.0%
ACCCAAATCAACGTTAACAA+TGG - Chr4:54820001-54820020 None:intergenic 35.0%
AGACGAGAAAAAATCTCAAG+AGG + Chr4:54820818-54820837 MsG0480021183.01.T01:intron 35.0%
AGATCATGAGGTAGTTTCTT+GGG + Chr4:54820595-54820614 MsG0480021183.01.T01:intron 35.0%
ATACAATTAGGGATGGCAAA+AGG - Chr4:54820892-54820911 None:intergenic 35.0%
ATCCCATTTATATCGGATCA+GGG + Chr4:54820031-54820050 MsG0480021183.01.T01:five_prime_UTR 35.0%
CAAATCAACGTTAACAATGG+TGG - Chr4:54819998-54820017 None:intergenic 35.0%
CAAGGTTCTATATTTAGGGA+TGG - Chr4:54820411-54820430 None:intergenic 35.0%
CACTCTCAACAGAAACATTT+GGG - Chr4:54821089-54821108 None:intergenic 35.0%
CATTAGATACATTACCAGAG+AGG + Chr4:54821640-54821659 MsG0480021183.01.T01:CDS 35.0%
CATTGTAATGCCACTCTTAT+AGG - Chr4:54820294-54820313 None:intergenic 35.0%
CCTTATTGTCACTAACACTA+CGG + Chr4:54821015-54821034 MsG0480021183.01.T01:five_prime_UTR 35.0%
GAGTAGGACACTATACAATT+AGG - Chr4:54820904-54820923 None:intergenic 35.0%
GTAAAAAAGCTTTACACCGA+CGG + Chr4:54820333-54820352 MsG0480021183.01.T01:intron 35.0%
GTGCATGTTTGTAATTGAGT+AGG - Chr4:54820920-54820939 None:intergenic 35.0%
GTTGTTGATGCTTTAACAAG+TGG + Chr4:54821060-54821079 MsG0480021183.01.T01:five_prime_UTR 35.0%
TATCCCATTTATATCGGATC+AGG + Chr4:54820030-54820049 MsG0480021183.01.T01:five_prime_UTR 35.0%
TATGTACAGTGTAACAGACA+TGG - Chr4:54821830-54821849 None:intergenic 35.0%
TGTGTTTCGTGAACTATTCA+TGG - Chr4:54820536-54820555 None:intergenic 35.0%
! CAATTGACTTTGTTGCTGTT+CGG + Chr4:54820562-54820581 MsG0480021183.01.T01:intron 35.0%
! CACCATTGTTAACGTTGATT+TGG + Chr4:54819996-54820015 MsG0480021183.01.T01:five_prime_UTR 35.0%
!! AAAGTTAGGATTTTGAAGGG+AGG - Chr4:54819833-54819852 None:intergenic 35.0%
!! AATGTACTTTTGGCTGGAAA+AGG + Chr4:54821860-54821879 MsG0480021183.01.T01:intron 35.0%
!! GATTAATTTGCATATGCCGT+CGG - Chr4:54820352-54820371 None:intergenic 35.0%
!! TTGTAGGTTGATTCTTCAGT+TGG + Chr4:54821531-54821550 MsG0480021183.01.T01:intron 35.0%
!!! GGGATACAGAATTTTTTGAG+TGG + Chr4:54821709-54821728 MsG0480021183.01.T01:CDS 35.0%
ACCAATCAAGTTCTTCCCAA+GGG - Chr4:54821580-54821599 None:intergenic 40.0%
ACTTTGCATCCGATTGAGTA+AGG - Chr4:54822120-54822139 None:intergenic 40.0%
AGTCAGGGCTACATTGAATT+TGG + Chr4:54822383-54822402 MsG0480021183.01.T01:CDS 40.0%
ATAGCATATGCCAGTGCATT+AGG - Chr4:54822306-54822325 None:intergenic 40.0%
ATCATGAGGTAGTTTCTTGG+GGG + Chr4:54820597-54820616 MsG0480021183.01.T01:intron 40.0%
CAACCCTGATCCGATATAAA+TGG - Chr4:54820037-54820056 None:intergenic 40.0%
CAATAAGGACCTTCTTTCCA+TGG - Chr4:54821003-54821022 None:intergenic 40.0%
CATGGAAATTGAGCTATGAC+AGG - Chr4:54822090-54822109 None:intergenic 40.0%
CCGTAGTGTTAGTGACAATA+AGG - Chr4:54821018-54821037 None:intergenic 40.0%
CGAACAGCAACAAAGTCAAT+TGG - Chr4:54820564-54820583 None:intergenic 40.0%
CGGAGCTATCCCATTTATAT+CGG + Chr4:54820024-54820043 MsG0480021183.01.T01:five_prime_UTR 40.0%
CTATAAGCACACATTGAGGT+TGG - Chr4:54821611-54821630 None:intergenic 40.0%
CTCAAGAGGCTCAGATATTA+GGG + Chr4:54820832-54820851 MsG0480021183.01.T01:intron 40.0%
CTTTGCATCCGATTGAGTAA+GGG - Chr4:54822119-54822138 None:intergenic 40.0%
GAAGCCATTGGAATTGGAAT+TGG - Chr4:54819754-54819773 None:intergenic 40.0%
GAGATCATGAGGTAGTTTCT+TGG + Chr4:54820594-54820613 MsG0480021183.01.T01:intron 40.0%
GATCATGAGGTAGTTTCTTG+GGG + Chr4:54820596-54820615 MsG0480021183.01.T01:intron 40.0%
GGACACTATACAATTAGGGA+TGG - Chr4:54820899-54820918 None:intergenic 40.0%
GTCAGGGCTACATTGAATTT+GGG + Chr4:54822384-54822403 MsG0480021183.01.T01:CDS 40.0%
GTGGCAAGAGAAAAATATGC+AGG + Chr4:54821728-54821747 MsG0480021183.01.T01:CDS 40.0%
GTGTCTATAAGCACACATTG+AGG - Chr4:54821615-54821634 None:intergenic 40.0%
TAGGAAGTAGCCTATAAGAG+TGG + Chr4:54820281-54820300 MsG0480021183.01.T01:intron 40.0%
TCAGATTCCAACCACTGTTA+TGG + Chr4:54821370-54821389 MsG0480021183.01.T01:CDS 40.0%
TCTCAAGAGGCTCAGATATT+AGG + Chr4:54820831-54820850 MsG0480021183.01.T01:intron 40.0%
TCTCTCTTTCTACACCTCTT+AGG + Chr4:54819905-54819924 MsG0480021183.01.T01:five_prime_UTR 40.0%
TGATTGCGTGTTCAACTGAT+AGG + Chr4:54821208-54821227 MsG0480021183.01.T01:intron 40.0%
TGTCATAGACTTGAACCATC+AGG - Chr4:54821468-54821487 None:intergenic 40.0%
TTGCACCTTGTATCTAGACA+AGG + Chr4:54821038-54821057 MsG0480021183.01.T01:five_prime_UTR 40.0%
TTTCCAGCAAAAATCCAACC+TGG + Chr4:54819967-54819986 MsG0480021183.01.T01:five_prime_UTR 40.0%
! AGGTTGGATTTTTGCTGGAA+AGG - Chr4:54819968-54819987 None:intergenic 40.0%
! GGTTGGATTTTTGCTGGAAA+GGG - Chr4:54819967-54819986 None:intergenic 40.0%
! GTTGGATTTTTGCTGGAAAG+GGG - Chr4:54819966-54819985 None:intergenic 40.0%
! TTGGGAAATGTACTTTTGGC+TGG + Chr4:54821854-54821873 MsG0480021183.01.T01:intron 40.0%
!! AATTGGTTGTTGTTTGACGG+AGG - Chr4:54819803-54819822 None:intergenic 40.0%
!! ATTGGTTGTTGTTTGACGGA+GGG - Chr4:54819802-54819821 None:intergenic 40.0%
!! GAGAGTGTAATTTTACCGGA+TGG + Chr4:54821102-54821121 MsG0480021183.01.T01:five_prime_UTR 40.0%
!! GGAATTGGAATTTTGGTGGT+TGG - Chr4:54819739-54819758 None:intergenic 40.0%
!! TAGGTTGATTCTTCAGTTGG+TGG + Chr4:54821534-54821553 MsG0480021183.01.T01:intron 40.0%
AACACCACGTAGGAATGAAG+CGG - Chr4:54821342-54821361 None:intergenic 45.0%
ACTGGTATAAACCATCCCCT+TGG + Chr4:54821561-54821580 MsG0480021183.01.T01:CDS 45.0%
AGTTTCTTGGGGGTGATACA+TGG + Chr4:54820607-54820626 MsG0480021183.01.T01:intron 45.0%
ATATGCAGGCATTAATGGCC+AGG + Chr4:54821742-54821761 MsG0480021183.01.T01:CDS 45.0%
ATCATGTGAAAACAAGGCCG+AGG + Chr4:54822332-54822351 MsG0480021183.01.T01:CDS 45.0%
CACCAATCAAGTTCTTCCCA+AGG - Chr4:54821581-54821600 None:intergenic 45.0%
CCAATCAAGTTCTTCCCAAG+GGG - Chr4:54821579-54821598 None:intergenic 45.0%
CCATTGAGTCTCGATTGGAT+CGG + Chr4:54821258-54821277 MsG0480021183.01.T01:CDS 45.0%
CTGGTATAAACCATCCCCTT+GGG + Chr4:54821562-54821581 MsG0480021183.01.T01:CDS 45.0%
GATATCGTGATGTTTACGCG+AGG + Chr4:54820639-54820658 MsG0480021183.01.T01:intron 45.0%
GGAATAAACGCGAGATCATG+AGG + Chr4:54820583-54820602 MsG0480021183.01.T01:intron 45.0%
GTGCATTAGGATCTCTGCAA+TGG - Chr4:54822293-54822312 None:intergenic 45.0%
GTTTCTTGGGGGTGATACAT+GGG + Chr4:54820608-54820627 MsG0480021183.01.T01:intron 45.0%
TAAGACCTGTGCCATAACAG+TGG - Chr4:54821384-54821403 None:intergenic 45.0%
TACATTACCAGAGAGGGAAC+TGG + Chr4:54821647-54821666 MsG0480021183.01.T01:CDS 45.0%
TAGGCATGTCCATGGAAAGA+AGG + Chr4:54820991-54821010 MsG0480021183.01.T01:intron 45.0%
TATGCGCTGTGTTATCCTGA+TGG + Chr4:54821450-54821469 MsG0480021183.01.T01:intron 45.0%
TGAAGGGAGGAGAGATGAAT+TGG - Chr4:54819820-54819839 None:intergenic 45.0%
TGAGAGGAACTAGCGCATAT+TGG - Chr4:54819934-54819953 None:intergenic 45.0%
TGTGTGCTCCCTTACTCAAT+CGG + Chr4:54822108-54822127 MsG0480021183.01.T01:intron 45.0%
TTGCAGAGATCCTAATGCAC+TGG + Chr4:54822293-54822312 MsG0480021183.01.T01:intron 45.0%
TTTCTTGGGGGTGATACATG+GGG + Chr4:54820609-54820628 MsG0480021183.01.T01:intron 45.0%
! ATTGAGTAAGGGAGCACACA+TGG - Chr4:54822108-54822127 None:intergenic 45.0%
!! AAAAATGGTTGGTGGGGTGA+GGG - Chr4:54819876-54819895 None:intergenic 45.0%
!! ACACCAGGTTGGATTTTTGC+TGG - Chr4:54819973-54819992 None:intergenic 45.0%
!! TCTTCAGTTGGTGGCAAAAC+TGG + Chr4:54821543-54821562 MsG0480021183.01.T01:CDS 45.0%
!! TGGAAAAGGCATCTCTAGCT+TGG + Chr4:54821874-54821893 MsG0480021183.01.T01:intron 45.0%
!! TTGGTTGTTGTTTGACGGAG+GGG - Chr4:54819801-54819820 None:intergenic 45.0%
!! TTTAAAAGAAAATTAAAATA+GGG + Chr4:54821986-54822005 MsG0480021183.01.T01:intron 5.0%
!!! TTTTAAAAGAAAATTAAAAT+AGG + Chr4:54821985-54822004 MsG0480021183.01.T01:intron 5.0%
ACCTGTGCCATAACAGTGGT+TGG - Chr4:54821380-54821399 None:intergenic 50.0%
ATGAGAGAGAAGGTGGAGTC+AGG + Chr4:54822367-54822386 MsG0480021183.01.T01:CDS 50.0%
CAAGGCCATTGAGTCTCGAT+TGG + Chr4:54821253-54821272 MsG0480021183.01.T01:CDS 50.0%
CCCCTTGGGAAGAACTTGAT+TGG + Chr4:54821576-54821595 MsG0480021183.01.T01:CDS 50.0%
CCGATCCAATCGAGACTCAA+TGG - Chr4:54821261-54821280 None:intergenic 50.0%
CGCTAGTTCCTCTCAACTCA+TGG + Chr4:54819939-54819958 MsG0480021183.01.T01:five_prime_UTR 50.0%
CTCATCCAAGGACACAACCT+CGG - Chr4:54822352-54822371 None:intergenic 50.0%
GAGGCTCAGATATTAGGGAC+AGG + Chr4:54820837-54820856 MsG0480021183.01.T01:intron 50.0%
GGTGTGGAAGCCATTGGAAT+TGG - Chr4:54819760-54819779 None:intergenic 50.0%
GTGTCCTTGGATGAGAGAGA+AGG + Chr4:54822357-54822376 MsG0480021183.01.T01:CDS 50.0%
TCAAGTTCTTCCCAAGGGGA+TGG - Chr4:54821575-54821594 None:intergenic 50.0%
TCCAACCACTGTTATGGCAC+AGG + Chr4:54821376-54821395 MsG0480021183.01.T01:CDS 50.0%
TCCACCTTCTCTCTCATCCA+AGG - Chr4:54822364-54822383 None:intergenic 50.0%
TCCTTGGATGAGAGAGAAGG+TGG + Chr4:54822360-54822379 MsG0480021183.01.T01:CDS 50.0%
TCCTTGTACTGCTCACCATC+CGG - Chr4:54821120-54821139 None:intergenic 50.0%
TGAGAGAGAAGGTGGAGTCA+GGG + Chr4:54822368-54822387 MsG0480021183.01.T01:CDS 50.0%
TGGTGAGCAGTACAAGGACA+TGG + Chr4:54821122-54821141 MsG0480021183.01.T01:exon 50.0%
TTGAGTCTCGATTGGATCGG+CGG + Chr4:54821261-54821280 MsG0480021183.01.T01:CDS 50.0%
! AAAGGGGTCCATGAGTTGAG+AGG - Chr4:54819950-54819969 None:intergenic 50.0%
! ACTTTTGTTGCCCTTGGAGG+TGG + Chr4:54821287-54821306 MsG0480021183.01.T01:CDS 50.0%
! CTTTTGTTGCCCTTGGAGGT+GGG + Chr4:54821288-54821307 MsG0480021183.01.T01:CDS 50.0%
! GAGTGGGGGTGTAGAAAGTT+AGG - Chr4:54819847-54819866 None:intergenic 50.0%
! GCGCATATTGGTGACCTAAG+AGG - Chr4:54819922-54819941 None:intergenic 50.0%
! TGCACTTTTGTTGCCCTTGG+AGG + Chr4:54821284-54821303 MsG0480021183.01.T01:CDS 50.0%
! TTAACAATGGTGGGGACACC+AGG - Chr4:54819988-54820007 None:intergenic 50.0%
!! AAAATGGTTGGTGGGGTGAG+GGG - Chr4:54819875-54819894 None:intergenic 50.0%
!! GAAAAATGGTTGGTGGGGTG+AGG - Chr4:54819877-54819896 None:intergenic 50.0%
!! TGGTTGTTGTTTGACGGAGG+GGG - Chr4:54819800-54819819 None:intergenic 50.0%
ACCGGATGGTGAGCAGTACA+AGG + Chr4:54821116-54821135 MsG0480021183.01.T01:five_prime_UTR 55.0%
CAGAGAGGGAACTGGCATCA+GGG + Chr4:54821655-54821674 MsG0480021183.01.T01:CDS 55.0%
CCAATCACACCCACCTCCAA+GGG - Chr4:54821300-54821319 None:intergenic 55.0%
GAAGGAGAATTGGGTGGGTG+TGG - Chr4:54819776-54819795 None:intergenic 55.0%
GGAGGGGGAGAAGGAGAATT+GGG - Chr4:54819785-54819804 None:intergenic 55.0%
GGGGAGAAGGAGAATTGGGT+GGG - Chr4:54819781-54819800 None:intergenic 55.0%
GGTGTGATTGGCGACATGTG+TGG + Chr4:54821309-54821328 MsG0480021183.01.T01:CDS 55.0%
TTGTTTGACGGAGGGGGAGA+AGG - Chr4:54819794-54819813 None:intergenic 55.0%
! CGGTGCACTTTTGTTGCCCT+TGG + Chr4:54821281-54821300 MsG0480021183.01.T01:CDS 55.0%
CAAGGCCGAGGTTGTGTCCT+TGG + Chr4:54822344-54822363 MsG0480021183.01.T01:CDS 60.0%
CCAGAGAGGGAACTGGCATC+AGG + Chr4:54821654-54821673 MsG0480021183.01.T01:CDS 60.0%
CCCTTGGAGGTGGGTGTGAT+TGG + Chr4:54821297-54821316 MsG0480021183.01.T01:CDS 60.0%
CCTGATGCCAGTTCCCTCTC+TGG - Chr4:54821657-54821676 None:intergenic 60.0%
CGGAGGGGGAGAAGGAGAAT+TGG - Chr4:54819786-54819805 None:intergenic 60.0%
GCCAATCACACCCACCTCCA+AGG - Chr4:54821301-54821320 None:intergenic 60.0%
GGGGGAGAAGGAGAATTGGG+TGG - Chr4:54819782-54819801 None:intergenic 60.0%
GGGGTGAGGGGAGAAAGAGT+GGG - Chr4:54819863-54819882 None:intergenic 60.0%
GGGTGAGGGGAGAAAGAGTG+GGG - Chr4:54819862-54819881 None:intergenic 60.0%
GGTGAGGGGAGAAAGAGTGG+GGG - Chr4:54819861-54819880 None:intergenic 60.0%
TGGGGTGAGGGGAGAAAGAG+TGG - Chr4:54819864-54819883 None:intergenic 60.0%
TGGGTGGGTGTGGAAGCCAT+TGG - Chr4:54819766-54819785 None:intergenic 60.0%
! CAATGGTGGGGACACCAGGT+TGG - Chr4:54819984-54820003 None:intergenic 60.0%
! GCTGCCGCTTCATTCCTACG+TGG + Chr4:54821335-54821354 MsG0480021183.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 54819671 54822503 54819671 ID=MsG0480021183.01;Name=MsG0480021183.01
Chr4 mRNA 54819671 54822503 54819671 ID=MsG0480021183.01.T01;Parent=MsG0480021183.01;Name=MsG0480021183.01.T01;_AED=0.39;_eAED=0.40;_QI=559|1|0.8|1|0.75|0.6|5|0|200
Chr4 exon 54819671 54820082 54819671 ID=MsG0480021183.01.T01:exon:1061;Parent=MsG0480021183.01.T01
Chr4 exon 54820994 54821143 54820994 ID=MsG0480021183.01.T01:exon:1062;Parent=MsG0480021183.01.T01
Chr4 exon 54821230 54821397 54821230 ID=MsG0480021183.01.T01:exon:1063;Parent=MsG0480021183.01.T01
Chr4 exon 54821537 54821763 54821537 ID=MsG0480021183.01.T01:exon:1064;Parent=MsG0480021183.01.T01
Chr4 exon 54822299 54822503 54822299 ID=MsG0480021183.01.T01:exon:1065;Parent=MsG0480021183.01.T01
Chr4 five_prime_UTR 54819671 54820082 54819671 ID=MsG0480021183.01.T01:five_prime_utr;Parent=MsG0480021183.01.T01
Chr4 five_prime_UTR 54820994 54821140 54820994 ID=MsG0480021183.01.T01:five_prime_utr;Parent=MsG0480021183.01.T01
Chr4 CDS 54821141 54821143 54821141 ID=MsG0480021183.01.T01:cds;Parent=MsG0480021183.01.T01
Chr4 CDS 54821230 54821397 54821230 ID=MsG0480021183.01.T01:cds;Parent=MsG0480021183.01.T01
Chr4 CDS 54821537 54821763 54821537 ID=MsG0480021183.01.T01:cds;Parent=MsG0480021183.01.T01
Chr4 CDS 54822299 54822503 54822299 ID=MsG0480021183.01.T01:cds;Parent=MsG0480021183.01.T01
Gene Sequence

>MsG0480021183.01.T01

ATGGATACTCTTATGAAAGTGTTTGACAAGGCCATTGAGTCTCGATTGGATCGGCGGTGCACTTTTGTTGCCCTTGGAGGTGGGTGTGATTGGCGACATGTGTGGCTTGCTGCCGCTTCATTCCTACGTGGTGTTAATTTTATTCAGATTCCAACCACTGTTATGGCACAGGTTGATTCTTCAGTTGGTGGCAAAACTGGTATAAACCATCCCCTTGGGAAGAACTTGATTGGTGCTTTTTACCAACCTCAATGTGTGCTTATAGACACAGACACATTAGATACATTACCAGAGAGGGAACTGGCATCAGGGTTTGCAGAAGTTATAAAGTATGGGCTCATTAGGGATACAGAATTTTTTGAGTGGCAAGAGAAAAATATGCAGGCATTAATGGCCAGAGATCCTAATGCACTGGCATATGCTATAAAGCGATCATGTGAAAACAAGGCCGAGGTTGTGTCCTTGGATGAGAGAGAAGGTGGAGTCAGGGCTACATTGAATTTGGGTCATACATTTGGCCATGTGAGTAGTTTTACTGTTTATTATATTATTTGGATGCAAAACTTTGCTGTGAAGACAATCCTTTTCTTTCTTGATGTATGA
Protein sequence

>MsG0480021183.01.T01

MDTLMKVFDKAIESRLDRRCTFVALGGGCDWRHVWLAAASFLRGVNFIQIPTTVMAQVDSSVGGKTGINHPLGKNLIGAFYQPQCVLIDTDTLDTLPERELASGFAEVIKYGLIRDTEFFEWQEKNMQALMARDPNALAYAIKRSCENKAEVVSLDEREGGVRATLNLGHTFGHVSSFTVYYIIWMQNFAVKTILFFLDV*