AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021218.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021218.01.T01 MTR_4g069140 95.789 380 8 1 1 380 1 372 0.0 746
MsG0480021218.01.T01 MTR_1g021925 60.533 375 139 2 1 374 1 367 4.99e-167 472
MsG0480021218.01.T01 MTR_1g052685 36.820 239 140 4 98 334 84 313 1.77e-45 160
MsG0480021218.01.T01 MTR_7g092280 32.031 256 163 3 96 348 82 329 1.71e-43 155
MsG0480021218.01.T01 MTR_2g097980 36.019 211 115 3 96 306 82 272 1.34e-39 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021218.01.T01 AT3G18850 61.813 364 129 2 18 379 12 367 5.09e-173 487
MsG0480021218.01.T01 AT3G18850 61.813 364 129 2 18 379 12 367 5.09e-173 487
MsG0480021218.01.T01 AT3G18850 61.813 364 129 2 18 379 12 367 5.09e-173 487
MsG0480021218.01.T01 AT3G18850 61.813 364 129 2 18 379 12 367 5.09e-173 487
MsG0480021218.01.T01 AT3G18850 61.791 335 118 2 47 379 18 344 1.31e-158 449
MsG0480021218.01.T01 AT1G75020 59.128 367 139 3 14 379 13 369 1.65e-154 440
MsG0480021218.01.T01 AT1G75020 59.128 367 139 3 14 379 13 369 1.65e-154 440
MsG0480021218.01.T01 AT3G18850 54.825 228 93 2 154 379 17 236 9.29e-91 273
MsG0480021218.01.T01 AT3G57650 35.628 247 147 3 97 339 82 320 3.92e-46 162
MsG0480021218.01.T01 AT1G51260 29.938 324 207 8 28 345 12 321 3.91e-38 141

Find 94 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTCGACTGGATGTATTTA+TGG 0.192495 4:+55282409 MsG0480021218.01.T01:CDS
TCTTTCTTGGACAATGTATT+TGG 0.227806 4:+55283461 MsG0480021218.01.T01:CDS
AGAAAGTGAGGTGTAGATTT+TGG 0.233514 4:-55280437 None:intergenic
GATGGATAGATTCCGATTTA+AGG 0.239062 4:+55283571 MsG0480021218.01.T01:CDS
GTCTGTAGTCGTCGCTTATT+TGG 0.253949 4:+55283757 MsG0480021218.01.T01:CDS
ATACCGCTGCCCGTCTTTCT+TGG 0.257600 4:+55283448 MsG0480021218.01.T01:CDS
TACGTAAAATCAGTGCCTTC+AGG 0.315013 4:-55282636 None:intergenic
AAGGGGTTTGGTGTGTCTAT+TGG 0.322223 4:+55282155 MsG0480021218.01.T01:CDS
ATTCGGCCACAACCCATTCT+TGG 0.333164 4:+55283800 MsG0480021218.01.T01:CDS
CTTGTTTCTGATAGCATATA+TGG 0.341553 4:+55282065 MsG0480021218.01.T01:exon
TGAAATTTCTACGTGGTTGA+TGG 0.356721 4:+55283553 MsG0480021218.01.T01:CDS
AAACCCCTTAGAAATCTAAA+AGG 0.358797 4:-55282141 None:intergenic
GCTTCCCACTTCCTTTCCAC+TGG 0.362237 4:-55282540 None:intergenic
TTTGGTGTGTCTATTGGTGC+TGG 0.382243 4:+55282161 MsG0480021218.01.T01:CDS
GGATGGGAAAGCGGCAGATA+TGG 0.384803 4:-55283508 None:intergenic
CGGCTACATATTTCAACATT+CGG 0.392577 4:+55283783 MsG0480021218.01.T01:CDS
AGTCGTCGCTTATTTGGTTC+CGG 0.394861 4:+55283763 MsG0480021218.01.T01:CDS
AGCAAGCCAGAGAGGATCTT+GGG 0.400056 4:-55282607 None:intergenic
TTTAAAATGCCCTTCACAGC+AGG 0.401277 4:-55283659 None:intergenic
TTGGTACTTGGCTAACTTTG+TGG 0.403348 4:+55282286 MsG0480021218.01.T01:CDS
TTTGTAACATTGGTGTTCTT+CGG 0.407274 4:+55282195 MsG0480021218.01.T01:CDS
AAAGCAACTTGTCCTTAAAT+CGG 0.413361 4:-55283583 None:intergenic
GAAATTTCATCTTCAGATGT+TGG 0.414236 4:-55283539 None:intergenic
AAATTTCATCTTCAGATGTT+GGG 0.418091 4:-55283538 None:intergenic
CTTCCCACTTCCTTTCCACT+GGG 0.434035 4:-55282539 None:intergenic
AAGACTAAAGTTGTGTTCTC+TGG 0.436607 4:+55282333 MsG0480021218.01.T01:CDS
ACAAGAGCTTGATGAGAGAT+TGG 0.437491 4:-55280533 None:intergenic
TACAAGAAGAAAATGATAGA+TGG 0.444101 4:-55283005 None:intergenic
GCCTCGCAACTCTTGCAAAC+AGG 0.445731 4:-55283124 None:intergenic
ACGCGTGTAACTTCAAAACT+TGG 0.456940 4:+55282096 MsG0480021218.01.T01:CDS
AGTCGACTGGATGTATTTAT+GGG 0.457387 4:+55282410 MsG0480021218.01.T01:CDS
TTCATCAGCATTTGTAACAT+TGG 0.466713 4:+55282185 MsG0480021218.01.T01:CDS
TAGCAAGCCAGAGAGGATCT+TGG 0.476050 4:-55282608 None:intergenic
TTGATCTCAATGTTTGTTTG+CGG 0.479384 4:+55280558 MsG0480021218.01.T01:five_prime_UTR
ATGATCCCCAAGATCCTCTC+TGG 0.492077 4:+55282601 MsG0480021218.01.T01:CDS
AAGATAGAATTAAGAAAGTG+AGG 0.497353 4:-55280449 None:intergenic
ACGTAAAATCAGTGCCTTCA+GGG 0.505273 4:-55282635 None:intergenic
TCAGTTTACGATGTTACCAT+TGG 0.507137 4:+55283419 MsG0480021218.01.T01:intron
GAGGGACCTCCCTGCTGTGA+AGG 0.507648 4:+55283649 MsG0480021218.01.T01:CDS
CAAACAGGTACAAAAGCCCT+TGG 0.513559 4:-55283109 None:intergenic
TCATCCCAGTGGAAAGGAAG+TGG 0.514537 4:+55282535 MsG0480021218.01.T01:CDS
ATATTCCGTTCTGTCAATCC+AGG 0.515335 4:+55282013 MsG0480021218.01.T01:five_prime_UTR
GCACCTAGGGGAAAGTAGAC+TGG 0.517923 4:-55281903 None:intergenic
ATACAATATATTAACAAGCC+TGG 0.520410 4:-55282031 None:intergenic
CTTGGACAATGTATTTGGAG+TGG 0.522752 4:+55283466 MsG0480021218.01.T01:CDS
ATGGGACCTTGCGTTGCGGA+AGG 0.532796 4:+55282428 MsG0480021218.01.T01:CDS
AGATGTTGGGATACAGTGGA+TGG 0.536671 4:-55283525 None:intergenic
CATCCAGTCTACTTTCCCCT+AGG 0.538508 4:+55281900 MsG0480021218.01.T01:five_prime_UTR
GATACGATAGATCGCACCTA+GGG 0.544055 4:-55281916 None:intergenic
ACATCCAGTCGACTTCAGTT+CGG 0.547137 4:-55282401 None:intergenic
AGGGCTAAGGAAACAATCTT+CGG 0.547167 4:-55283932 None:intergenic
AGGGACCTCCCTGCTGTGAA+GGG 0.552077 4:+55283650 MsG0480021218.01.T01:CDS
GTGACAGTGATTATATTGAC+TGG 0.553389 4:+55283686 MsG0480021218.01.T01:CDS
ATATTGTTCCTTCGAGAGAA+CGG 0.553559 4:+55282358 MsG0480021218.01.T01:CDS
TGTCCAAGAAAGACGGGCAG+CGG 0.556324 4:-55283451 None:intergenic
ATCACCGAACTGAAGTCGAC+TGG 0.565240 4:+55282397 MsG0480021218.01.T01:CDS
TGGGACCTTGCGTTGCGGAA+GGG 0.565717 4:+55282429 MsG0480021218.01.T01:CDS
ATACATTGTCCAAGAAAGAC+GGG 0.565950 4:-55283457 None:intergenic
TTGCGAGGCTCTCTAAATGC+AGG 0.568276 4:+55283138 MsG0480021218.01.T01:CDS
TGCATCCCTTCCGCAACGCA+AGG 0.570372 4:-55282434 None:intergenic
AGCACAATCCGTTCTCTCGA+AGG 0.580059 4:-55282366 None:intergenic
AATACATTGTCCAAGAAAGA+CGG 0.589227 4:-55283458 None:intergenic
CGAATGTTGAAATATGTAGC+CGG 0.590553 4:-55283782 None:intergenic
ATGTACAAGGACAAAGCCAC+TGG 0.592436 4:+55281939 MsG0480021218.01.T01:five_prime_UTR
TCTGCATATCACTCTGCCAG+TGG 0.604292 4:-55281955 None:intergenic
CTTCAGATGTTGGGATACAG+TGG 0.606141 4:-55283529 None:intergenic
ACCTGTTTGCAAGAGTTGCG+AGG 0.606957 4:+55283123 MsG0480021218.01.T01:CDS
TTAAAATGCCCTTCACAGCA+GGG 0.607039 4:-55283658 None:intergenic
ACAAGCCTGGATTGACAGAA+CGG 0.609134 4:-55282018 None:intergenic
TAGAGTTCATCCCAGTGGAA+AGG 0.609644 4:+55282529 MsG0480021218.01.T01:CDS
GCAGATATGGATATGAACTT+CGG 0.613202 4:-55283495 None:intergenic
CATCCCAGTGGAAAGGAAGT+GGG 0.613686 4:+55282536 MsG0480021218.01.T01:CDS
AATGCTACAAGCTTGATCAA+AGG 0.616079 4:+55280588 MsG0480021218.01.T01:five_prime_UTR
GGATACAGTGGATGGGAAAG+CGG 0.620018 4:-55283517 None:intergenic
ATATGGATATGAACTTCGGA+AGG 0.623808 4:-55283491 None:intergenic
GATCTATCGTATCATGTACA+AGG 0.624221 4:+55281926 MsG0480021218.01.T01:five_prime_UTR
GCGTTGCGGAAGGGATGCAT+TGG 0.626115 4:+55282438 MsG0480021218.01.T01:CDS
TCCTGATCAAGCAAAAGAGA+GGG 0.626456 4:+55283631 MsG0480021218.01.T01:CDS
GGGAAAATAGCAAGCCAGAG+AGG 0.628084 4:-55282615 None:intergenic
TTCCTGATCAAGCAAAAGAG+AGG 0.628899 4:+55283630 MsG0480021218.01.T01:CDS
GCAGCGGTATTTGTAGCCAA+TGG 0.634421 4:-55283435 None:intergenic
GTTTCTGATAGCATATATGG+CGG 0.637213 4:+55282068 MsG0480021218.01.T01:exon
AAAACTCCAAGAATGGGTTG+TGG 0.641853 4:-55283806 None:intergenic
GATGTTGGGATACAGTGGAT+GGG 0.645947 4:-55283524 None:intergenic
TGATACGATAGATCGCACCT+AGG 0.651079 4:-55281917 None:intergenic
GCAAGCCAGAGAGGATCTTG+GGG 0.667193 4:-55282606 None:intergenic
ATTTATGGGACCTTGCGTTG+CGG 0.679165 4:+55282424 MsG0480021218.01.T01:CDS
GATATGCAGAACAAACTCCA+AGG 0.686790 4:+55281968 MsG0480021218.01.T01:five_prime_UTR
GTAAAGTTTAAACCAGACAG+AGG 0.706270 4:-55283732 None:intergenic
CATACTAGAGTTCATCCCAG+TGG 0.720097 4:+55282524 MsG0480021218.01.T01:CDS
AAATGCCCTTCACAGCAGGG+AGG 0.725667 4:-55283655 None:intergenic
ATACGATAGATCGCACCTAG+GGG 0.736068 4:-55281915 None:intergenic
ATTCACTATTCATCAAAACG+AGG 0.738583 4:+55280481 MsG0480021218.01.T01:five_prime_UTR
CTGAAGATGAAATTTCTACG+TGG 0.745759 4:+55283546 MsG0480021218.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGAAAATCTATTAAAAAG+TGG - Chr4:55282664-55282683 None:intergenic 15.0%
!!! ATCATTTTTATTTTTTCTTC+TGG - Chr4:55281557-55281576 None:intergenic 15.0%
!!! TCATTTTTATTTTTTCTTCT+GGG - Chr4:55281556-55281575 None:intergenic 15.0%
!!! TTGATTTTTTCAAATAGAAA+GGG - Chr4:55282312-55282331 None:intergenic 15.0%
!! ATCTATTCAAAATTATGACT+AGG - Chr4:55281033-55281052 None:intergenic 20.0%
!! CTATCATTGCTTATAATAAA+GGG - Chr4:55281712-55281731 None:intergenic 20.0%
!! CTTAAAAAATTGTCTGAATT+TGG - Chr4:55282714-55282733 None:intergenic 20.0%
!! GAATATCTTCTATGAAAAAT+TGG - Chr4:55281999-55282018 None:intergenic 20.0%
!!! AGTAAAACATTTTTCAGAAT+CGG - Chr4:55283081-55283100 None:intergenic 20.0%
!!! ATCTTTTTACTTTACTTTCT+CGG + Chr4:55281151-55281170 MsG0480021218.01.T01:intron 20.0%
!!! CTTTACAATTTTCAATTTCA+AGG + Chr4:55283602-55283621 MsG0480021218.01.T01:CDS 20.0%
!!! GTTGATTTTTTCAAATAGAA+AGG - Chr4:55282313-55282332 None:intergenic 20.0%
! AAATTTCATCTTCAGATGTT+GGG - Chr4:55283541-55283560 None:intergenic 25.0%
! AACCAATAATTGCATATACA+TGG - Chr4:55283187-55283206 None:intergenic 25.0%
! AAGATAATTCTACCATTTAC+TGG - Chr4:55281385-55281404 None:intergenic 25.0%
! AAGATAGAATTAAGAAAGTG+AGG - Chr4:55280452-55280471 None:intergenic 25.0%
! AATGATATCAGATTGATAGA+AGG + Chr4:55283330-55283349 MsG0480021218.01.T01:intron 25.0%
! ACATAATATCGCAAAAATGT+TGG + Chr4:55281620-55281639 MsG0480021218.01.T01:intron 25.0%
! AGATTGATAGAAGGTTAAAA+AGG + Chr4:55283339-55283358 MsG0480021218.01.T01:intron 25.0%
! ATACAATATATTAACAAGCC+TGG - Chr4:55282034-55282053 None:intergenic 25.0%
! ATCTTAAAAACTCCAAGAAT+GGG - Chr4:55283815-55283834 None:intergenic 25.0%
! CACAATTCAAATAGTTAATG+AGG + Chr4:55282741-55282760 MsG0480021218.01.T01:intron 25.0%
! GCTATCATTGCTTATAATAA+AGG - Chr4:55281713-55281732 None:intergenic 25.0%
! TACAAGAAGAAAATGATAGA+TGG - Chr4:55283008-55283027 None:intergenic 25.0%
! TACCATATAAGGAAGAAAAA+TGG - Chr4:55280788-55280807 None:intergenic 25.0%
! TACTTTGTATGTAATTCAGT+TGG + Chr4:55281217-55281236 MsG0480021218.01.T01:intron 25.0%
! TGTTACCTCAAAATTCAAAA+GGG - Chr4:55281450-55281469 None:intergenic 25.0%
! TTACTTTCTCGGATTATTAT+TGG + Chr4:55281162-55281181 MsG0480021218.01.T01:intron 25.0%
! TTCCATGTATATGCAATTAT+TGG + Chr4:55283182-55283201 MsG0480021218.01.T01:intron 25.0%
! TTGTTACCTCAAAATTCAAA+AGG - Chr4:55281451-55281470 None:intergenic 25.0%
!! TAGAGTTATATGATGTCTTT+TGG + Chr4:55280971-55280990 MsG0480021218.01.T01:intron 25.0%
!! TTATATGATGTCTTTTGGTA+TGG + Chr4:55280976-55280995 MsG0480021218.01.T01:intron 25.0%
!! TTTTGCGATATTATGTATCA+TGG - Chr4:55281616-55281635 None:intergenic 25.0%
!!! TTCCATTTTTCTTCCTTATA+TGG + Chr4:55280783-55280802 MsG0480021218.01.T01:intron 25.0%
AAACCCCTTAGAAATCTAAA+AGG - Chr4:55282144-55282163 None:intergenic 30.0%
AAAGCAACTTGTCCTTAAAT+CGG - Chr4:55283586-55283605 None:intergenic 30.0%
AAAGCCTAAGCATACATAAT+AGG - Chr4:55281793-55281812 None:intergenic 30.0%
AAATGTAATGAAGGACTGAA+TGG - Chr4:55281868-55281887 None:intergenic 30.0%
AATACATTGTCCAAGAAAGA+CGG - Chr4:55283461-55283480 None:intergenic 30.0%
ACCATTCAATTGTTTGACAT+TGG - Chr4:55281528-55281547 None:intergenic 30.0%
ATAATCCTTGGTTGAGTATA+AGG - Chr4:55283243-55283262 None:intergenic 30.0%
ATTCACTATTCATCAAAACG+AGG + Chr4:55280481-55280500 MsG0480021218.01.T01:five_prime_UTR 30.0%
CATACATAATAGGAGAAGAA+GGG - Chr4:55281783-55281802 None:intergenic 30.0%
CATCTTAAAAACTCCAAGAA+TGG - Chr4:55283816-55283835 None:intergenic 30.0%
CCAGATTTCTATGTATTGTT+AGG + Chr4:55280915-55280934 MsG0480021218.01.T01:intron 30.0%
CCTAACAATACATAGAAATC+TGG - Chr4:55280918-55280937 None:intergenic 30.0%
CTACCATTTACTGGATTATT+AGG - Chr4:55281376-55281395 None:intergenic 30.0%
GAAATTTCATCTTCAGATGT+TGG - Chr4:55283542-55283561 None:intergenic 30.0%
GGATCACTTGAAAAATGATA+GGG + Chr4:55280997-55281016 MsG0480021218.01.T01:intron 30.0%
GTCTTTACCACAGAAAAAAA+AGG - Chr4:55280709-55280728 None:intergenic 30.0%
GTTCCTAATAATCCAGTAAA+TGG + Chr4:55281370-55281389 MsG0480021218.01.T01:intron 30.0%
TAAAAGTTCACGTCACTTAA+TGG + Chr4:55281280-55281299 MsG0480021218.01.T01:intron 30.0%
TCTTTCTTGGACAATGTATT+TGG + Chr4:55283461-55283480 MsG0480021218.01.T01:CDS 30.0%
TGGATCACTTGAAAAATGAT+AGG + Chr4:55280996-55281015 MsG0480021218.01.T01:intron 30.0%
TGTCAAAGTTGTAGTGAAAT+TGG - Chr4:55281476-55281495 None:intergenic 30.0%
TTCATCAGCATTTGTAACAT+TGG + Chr4:55282185-55282204 MsG0480021218.01.T01:CDS 30.0%
TTCTCCTATTATGTATGCTT+AGG + Chr4:55281786-55281805 MsG0480021218.01.T01:intron 30.0%
TTGAATGTAACTATGTGTGT+CGG - Chr4:55281132-55281151 None:intergenic 30.0%
TTGATCTCAATGTTTGTTTG+CGG + Chr4:55280558-55280577 MsG0480021218.01.T01:five_prime_UTR 30.0%
TTGCTTGATGAATGCTTTAT+TGG + Chr4:55280650-55280669 MsG0480021218.01.T01:intron 30.0%
! AGCTCCTTTTAGATTTCTAA+GGG + Chr4:55282137-55282156 MsG0480021218.01.T01:CDS 30.0%
! AGTCCTTCATTACATTTTTC+AGG + Chr4:55281871-55281890 MsG0480021218.01.T01:intron 30.0%
! GAAATCCCTTTTGAATTTTG+AGG + Chr4:55281442-55281461 MsG0480021218.01.T01:intron 30.0%
! GATTGGAAAATGAGCTTTTA+AGG - Chr4:55280519-55280538 None:intergenic 30.0%
! GGAAGTAACTATGATGTTTT+TGG + Chr4:55280745-55280764 MsG0480021218.01.T01:intron 30.0%
! GGAATCATATGATTTTGTCA+CGG + Chr4:55282838-55282857 MsG0480021218.01.T01:intron 30.0%
! GTCCTTCATTACATTTTTCA+GGG + Chr4:55281872-55281891 MsG0480021218.01.T01:intron 30.0%
! TAGCTCCTTTTAGATTTCTA+AGG + Chr4:55282136-55282155 MsG0480021218.01.T01:CDS 30.0%
! TATTGTTGTTGCATGTCTTT+AGG + Chr4:55281323-55281342 MsG0480021218.01.T01:intron 30.0%
! TGCTTGATGAATGCTTTATT+GGG + Chr4:55280651-55280670 MsG0480021218.01.T01:intron 30.0%
! TTGATGAGACTTCCAATTTT+TGG + Chr4:55282489-55282508 MsG0480021218.01.T01:CDS 30.0%
! TTTGTAACATTGGTGTTCTT+CGG + Chr4:55282195-55282214 MsG0480021218.01.T01:CDS 30.0%
! TTTTCCCTTTTTCTCTTTCT+GGG - Chr4:55280814-55280833 None:intergenic 30.0%
! TTTTTCCCTTTTTCTCTTTC+TGG - Chr4:55280815-55280834 None:intergenic 30.0%
!! CACTTAATGGAAAAGTACTT+TGG + Chr4:55281293-55281312 MsG0480021218.01.T01:intron 30.0%
!! CTTGTTTCTGATAGCATATA+TGG + Chr4:55282065-55282084 MsG0480021218.01.T01:exon 30.0%
!! GGATTATTATTGGTGATGTT+CGG + Chr4:55281172-55281191 MsG0480021218.01.T01:intron 30.0%
!! TTAATGGAAAAGTACTTTGG+TGG + Chr4:55281296-55281315 MsG0480021218.01.T01:intron 30.0%
!! TTTTAGATTTCTAAGGGGTT+TGG + Chr4:55282143-55282162 MsG0480021218.01.T01:CDS 30.0%
!!! AAAACAACGTCGTTTTTCTT+TGG + Chr4:55282267-55282286 MsG0480021218.01.T01:CDS 30.0%
!!! AACTATGATGTTTTTGGAAC+TGG + Chr4:55280751-55280770 MsG0480021218.01.T01:intron 30.0%
!!! ACTATGATGTTTTTGGAACT+GGG + Chr4:55280752-55280771 MsG0480021218.01.T01:intron 30.0%
!!! GTTCAAACCTTTTTTTTCTG+TGG + Chr4:55280699-55280718 MsG0480021218.01.T01:intron 30.0%
AAACCCCAGAAAGAGAAAAA+GGG + Chr4:55280807-55280826 MsG0480021218.01.T01:intron 35.0%
AAAGTCACCATTCGAAGTAT+GGG - Chr4:55282898-55282917 None:intergenic 35.0%
AAGACTAAAGTTGTGTTCTC+TGG + Chr4:55282333-55282352 MsG0480021218.01.T01:CDS 35.0%
AAGTCGACTGGATGTATTTA+TGG + Chr4:55282409-55282428 MsG0480021218.01.T01:CDS 35.0%
AATGCTACAAGCTTGATCAA+AGG + Chr4:55280588-55280607 MsG0480021218.01.T01:five_prime_UTR 35.0%
ACCACATTATGCAAATGACA+AGG - Chr4:55280845-55280864 None:intergenic 35.0%
AGTCGACTGGATGTATTTAT+GGG + Chr4:55282410-55282429 MsG0480021218.01.T01:CDS 35.0%
ATACATTGTCCAAGAAAGAC+GGG - Chr4:55283460-55283479 None:intergenic 35.0%
ATATGGATATGAACTTCGGA+AGG - Chr4:55283494-55283513 None:intergenic 35.0%
ATATTGTTCCTTCGAGAGAA+CGG + Chr4:55282358-55282377 MsG0480021218.01.T01:CDS 35.0%
ATGCAATGATGCATAATCCT+TGG - Chr4:55283255-55283274 None:intergenic 35.0%
CAACATCATTGAACTCCAAA+AGG - Chr4:55281687-55281706 None:intergenic 35.0%
CACCCTGAAAAATGTAATGA+AGG - Chr4:55281877-55281896 None:intergenic 35.0%
CCTTCGAAGTCAAAAATATG+CGG + Chr4:55283041-55283060 MsG0480021218.01.T01:CDS 35.0%
CGAATGTTGAAATATGTAGC+CGG - Chr4:55283785-55283804 None:intergenic 35.0%
CGGCTACATATTTCAACATT+CGG + Chr4:55283783-55283802 MsG0480021218.01.T01:CDS 35.0%
CTGAAGATGAAATTTCTACG+TGG + Chr4:55283546-55283565 MsG0480021218.01.T01:CDS 35.0%
GATCTATCGTATCATGTACA+AGG + Chr4:55281926-55281945 MsG0480021218.01.T01:five_prime_UTR 35.0%
GATGGATAGATTCCGATTTA+AGG + Chr4:55283571-55283590 MsG0480021218.01.T01:CDS 35.0%
GCAGATATGGATATGAACTT+CGG - Chr4:55283498-55283517 None:intergenic 35.0%
GCATACATAATAGGAGAAGA+AGG - Chr4:55281784-55281803 None:intergenic 35.0%
GCCAATGTCAAACAATTGAA+TGG + Chr4:55281524-55281543 MsG0480021218.01.T01:intron 35.0%
GCGATATTATGTATCATGGA+AGG - Chr4:55281612-55281631 None:intergenic 35.0%
GTAAAGTTTAAACCAGACAG+AGG - Chr4:55283735-55283754 None:intergenic 35.0%
GTAGTCAGGTTTCTTGTATT+TGG + Chr4:55282817-55282836 MsG0480021218.01.T01:intron 35.0%
GTGACAGTGATTATATTGAC+TGG + Chr4:55283686-55283705 MsG0480021218.01.T01:CDS 35.0%
GTTTCTGATAGCATATATGG+CGG + Chr4:55282068-55282087 MsG0480021218.01.T01:exon 35.0%
TAAACCCCAGAAAGAGAAAA+AGG + Chr4:55280806-55280825 MsG0480021218.01.T01:intron 35.0%
TCAGTTTACGATGTTACCAT+TGG + Chr4:55283419-55283438 MsG0480021218.01.T01:intron 35.0%
TCATAGTTACTTCCAAAGCT+CGG - Chr4:55280739-55280758 None:intergenic 35.0%
TGAAATGCCCAACCAAAAAT+TGG - Chr4:55282504-55282523 None:intergenic 35.0%
TGAAATTTCTACGTGGTTGA+TGG + Chr4:55283553-55283572 MsG0480021218.01.T01:CDS 35.0%
TTAGACCTTATACTCAACCA+AGG + Chr4:55283235-55283254 MsG0480021218.01.T01:intron 35.0%
TTCTAGTGCATCATGTCTTT+AGG + Chr4:55281254-55281273 MsG0480021218.01.T01:intron 35.0%
TTGAGATCGAAGACATATCT+AGG + Chr4:55281087-55281106 MsG0480021218.01.T01:intron 35.0%
! ACCTTGTCATTTGCATAATG+TGG + Chr4:55280841-55280860 MsG0480021218.01.T01:intron 35.0%
! AGAAAGTGAGGTGTAGATTT+TGG - Chr4:55280440-55280459 None:intergenic 35.0%
! ATGAAAAATTGGTCTAGCCT+TGG - Chr4:55281988-55282007 None:intergenic 35.0%
! GCTCCTTTTAGATTTCTAAG+GGG + Chr4:55282138-55282157 MsG0480021218.01.T01:CDS 35.0%
! GCTTGATGAATGCTTTATTG+GGG + Chr4:55280652-55280671 MsG0480021218.01.T01:intron 35.0%
! TGCTGTTTGATTCTTACTAC+TGG + Chr4:55281420-55281439 MsG0480021218.01.T01:intron 35.0%
! TTTCCCTTTTTCTCTTTCTG+GGG - Chr4:55280813-55280832 None:intergenic 35.0%
! TTTTTCTGTGTGATCACACA+TGG + Chr4:55280890-55280909 MsG0480021218.01.T01:intron 35.0%
!! ATCTAGGTTTTGACATGTGT+CGG + Chr4:55281103-55281122 MsG0480021218.01.T01:intron 35.0%
!! TTTCTGGGGTTTACCATATA+AGG - Chr4:55280799-55280818 None:intergenic 35.0%
!! TTTTTAGCATCAGTTACAGC+AGG + Chr4:55282244-55282263 MsG0480021218.01.T01:CDS 35.0%
!!! CTATGATGTTTTTGGAACTG+GGG + Chr4:55280753-55280772 MsG0480021218.01.T01:intron 35.0%
!!! GAGACTTCCAATTTTTGGTT+GGG + Chr4:55282494-55282513 MsG0480021218.01.T01:CDS 35.0%
!!! TGAGACTTCCAATTTTTGGT+TGG + Chr4:55282493-55282512 MsG0480021218.01.T01:CDS 35.0%
AAAAATATGCGGCTGAACAC+GGG + Chr4:55283052-55283071 MsG0480021218.01.T01:CDS 40.0%
AAGACTAAAGATCCGAGCTT+TGG + Chr4:55280724-55280743 MsG0480021218.01.T01:intron 40.0%
ACAAGAGCTTGATGAGAGAT+TGG - Chr4:55280536-55280555 None:intergenic 40.0%
ACGCGTGTAACTTCAAAACT+TGG + Chr4:55282096-55282115 MsG0480021218.01.T01:CDS 40.0%
ACGTAAAATCAGTGCCTTCA+GGG - Chr4:55282638-55282657 None:intergenic 40.0%
AGACATGATGCACTAGAATG+TGG - Chr4:55281252-55281271 None:intergenic 40.0%
ATATTCCGTTCTGTCAATCC+AGG + Chr4:55282013-55282032 MsG0480021218.01.T01:five_prime_UTR 40.0%
ATGTCTTCGATCTCAAGTGT+CGG - Chr4:55281083-55281102 None:intergenic 40.0%
CAAAAATATGCGGCTGAACA+CGG + Chr4:55283051-55283070 MsG0480021218.01.T01:CDS 40.0%
CAAAGTCACCATTCGAAGTA+TGG - Chr4:55282899-55282918 None:intergenic 40.0%
CTTGGACAATGTATTTGGAG+TGG + Chr4:55283466-55283485 MsG0480021218.01.T01:CDS 40.0%
GATATGCAGAACAAACTCCA+AGG + Chr4:55281968-55281987 MsG0480021218.01.T01:five_prime_UTR 40.0%
GATGGCTAAGAATCAGCAAA+AGG - Chr4:55282990-55283009 None:intergenic 40.0%
TACGTAAAATCAGTGCCTTC+AGG - Chr4:55282639-55282658 None:intergenic 40.0%
TCCTGATCAAGCAAAAGAGA+GGG + Chr4:55283631-55283650 MsG0480021218.01.T01:CDS 40.0%
TTAAAATGCCCTTCACAGCA+GGG - Chr4:55283661-55283680 None:intergenic 40.0%
TTCCTGATCAAGCAAAAGAG+AGG + Chr4:55283630-55283649 MsG0480021218.01.T01:CDS 40.0%
TTGGTACTTGGCTAACTTTG+TGG + Chr4:55282286-55282305 MsG0480021218.01.T01:CDS 40.0%
! AAGTGAGGTGTAGATTTTGG+TGG - Chr4:55280437-55280456 None:intergenic 40.0%
! CTTTTGCTTGATCAGGAAAC+TGG - Chr4:55283628-55283647 None:intergenic 40.0%
! GTTTTACTGCCGAAAACCAA+GGG + Chr4:55283093-55283112 MsG0480021218.01.T01:CDS 40.0%
! TACTCACTCTTCGATCCTTT+TGG + Chr4:55281669-55281688 MsG0480021218.01.T01:intron 40.0%
! TGTTTTACTGCCGAAAACCA+AGG + Chr4:55283092-55283111 MsG0480021218.01.T01:CDS 40.0%
! TTTAAAATGCCCTTCACAGC+AGG - Chr4:55283662-55283681 None:intergenic 40.0%
!! AAAACTCCAAGAATGGGTTG+TGG - Chr4:55283809-55283828 None:intergenic 40.0%
!! AGGTGTAGATTTTGGTGGAT+AGG - Chr4:55280432-55280451 None:intergenic 40.0%
!! GTACAAAAGCCCTTGGTTTT+CGG - Chr4:55283105-55283124 None:intergenic 40.0%
!! TGTTGCATGTCTTTAGGATG+TGG + Chr4:55281329-55281348 MsG0480021218.01.T01:intron 40.0%
!!! ACTTCACTTTTTCCTCTGTC+TGG + Chr4:55283720-55283739 MsG0480021218.01.T01:CDS 40.0%
!!! CCGCATATTTTTGACTTCGA+AGG - Chr4:55283044-55283063 None:intergenic 40.0%
!!! CGTCGTTTTTCTTTGGTACT+TGG + Chr4:55282274-55282293 MsG0480021218.01.T01:CDS 40.0%
ACAAGCCTGGATTGACAGAA+CGG - Chr4:55282021-55282040 None:intergenic 45.0%
AGATGTTGGGATACAGTGGA+TGG - Chr4:55283528-55283547 None:intergenic 45.0%
ATACGATAGATCGCACCTAG+GGG - Chr4:55281918-55281937 None:intergenic 45.0%
ATGTACAAGGACAAAGCCAC+TGG + Chr4:55281939-55281958 MsG0480021218.01.T01:five_prime_UTR 45.0%
ATTTATGGGACCTTGCGTTG+CGG + Chr4:55282424-55282443 MsG0480021218.01.T01:CDS 45.0%
CAAACAGGTACAAAAGCCCT+TGG - Chr4:55283112-55283131 None:intergenic 45.0%
CATACTAGAGTTCATCCCAG+TGG + Chr4:55282524-55282543 MsG0480021218.01.T01:CDS 45.0%
CTTCAGATGTTGGGATACAG+TGG - Chr4:55283532-55283551 None:intergenic 45.0%
GATACGATAGATCGCACCTA+GGG - Chr4:55281919-55281938 None:intergenic 45.0%
GATGTTGGGATACAGTGGAT+GGG - Chr4:55283527-55283546 None:intergenic 45.0%
GCTATCACCCATACTTCGAA+TGG + Chr4:55282888-55282907 MsG0480021218.01.T01:intron 45.0%
GCTTATGATCATCGTGATGC+TGG + Chr4:55282790-55282809 MsG0480021218.01.T01:intron 45.0%
GGAAGGCTATTGCAATTCAG+AGG - Chr4:55281595-55281614 None:intergenic 45.0%
GTCTGTAGTCGTCGCTTATT+TGG + Chr4:55283757-55283776 MsG0480021218.01.T01:CDS 45.0%
TAGAGTTCATCCCAGTGGAA+AGG + Chr4:55282529-55282548 MsG0480021218.01.T01:CDS 45.0%
TGATACGATAGATCGCACCT+AGG - Chr4:55281920-55281939 None:intergenic 45.0%
! ACATCCAGTCGACTTCAGTT+CGG - Chr4:55282404-55282423 None:intergenic 45.0%
! AGTCGTCGCTTATTTGGTTC+CGG + Chr4:55283763-55283782 MsG0480021218.01.T01:CDS 45.0%
! TCCCTCTCTTTTGCTTGATC+AGG - Chr4:55283635-55283654 None:intergenic 45.0%
! TTTGGTGTGTCTATTGGTGC+TGG + Chr4:55282161-55282180 MsG0480021218.01.T01:CDS 45.0%
!! AAGGGGTTTGGTGTGTCTAT+TGG + Chr4:55282155-55282174 MsG0480021218.01.T01:CDS 45.0%
!!! TGGCTTGCTATTTTCCCTGA+AGG + Chr4:55282621-55282640 MsG0480021218.01.T01:CDS 45.0%
ACCTGTTTGCAAGAGTTGCG+AGG + Chr4:55283123-55283142 MsG0480021218.01.T01:CDS 50.0%
AGCAAGCCAGAGAGGATCTT+GGG - Chr4:55282610-55282629 None:intergenic 50.0%
AGCACAATCCGTTCTCTCGA+AGG - Chr4:55282369-55282388 None:intergenic 50.0%
ATCACCGAACTGAAGTCGAC+TGG + Chr4:55282397-55282416 MsG0480021218.01.T01:CDS 50.0%
ATGATCCCCAAGATCCTCTC+TGG + Chr4:55282601-55282620 MsG0480021218.01.T01:CDS 50.0%
ATTCGGCCACAACCCATTCT+TGG + Chr4:55283800-55283819 MsG0480021218.01.T01:CDS 50.0%
CATCCAGTCTACTTTCCCCT+AGG + Chr4:55281900-55281919 MsG0480021218.01.T01:five_prime_UTR 50.0%
CATCCCAGTGGAAAGGAAGT+GGG + Chr4:55282536-55282555 MsG0480021218.01.T01:CDS 50.0%
CTTCCCACTTCCTTTCCACT+GGG - Chr4:55282542-55282561 None:intergenic 50.0%
GAGGCTCTCTAAATGCAGGT+TGG + Chr4:55283142-55283161 MsG0480021218.01.T01:intron 50.0%
GCAGCGGTATTTGTAGCCAA+TGG - Chr4:55283438-55283457 None:intergenic 50.0%
GGATACAGTGGATGGGAAAG+CGG - Chr4:55283520-55283539 None:intergenic 50.0%
GGGAAAATAGCAAGCCAGAG+AGG - Chr4:55282618-55282637 None:intergenic 50.0%
GTGATGCTGGAGAAGTAGTC+AGG + Chr4:55282803-55282822 MsG0480021218.01.T01:intron 50.0%
TAGCAAGCCAGAGAGGATCT+TGG - Chr4:55282611-55282630 None:intergenic 50.0%
TCATCCCAGTGGAAAGGAAG+TGG + Chr4:55282535-55282554 MsG0480021218.01.T01:CDS 50.0%
TCGGACTGTCGGTACTACAT+AGG - Chr4:55281064-55281083 None:intergenic 50.0%
TCTGCATATCACTCTGCCAG+TGG - Chr4:55281958-55281977 None:intergenic 50.0%
TTGCGAGGCTCTCTAAATGC+AGG + Chr4:55283138-55283157 MsG0480021218.01.T01:CDS 50.0%
!! GATCTCAAGTGTCGGACTGT+CGG - Chr4:55281075-55281094 None:intergenic 50.0%
AAATGCCCTTCACAGCAGGG+AGG - Chr4:55283658-55283677 None:intergenic 55.0%
ATACCGCTGCCCGTCTTTCT+TGG + Chr4:55283448-55283467 MsG0480021218.01.T01:CDS 55.0%
GCAAGCCAGAGAGGATCTTG+GGG - Chr4:55282609-55282628 None:intergenic 55.0%
GCACCTAGGGGAAAGTAGAC+TGG - Chr4:55281906-55281925 None:intergenic 55.0%
GCCTCGCAACTCTTGCAAAC+AGG - Chr4:55283127-55283146 None:intergenic 55.0%
GCTTCCCACTTCCTTTCCAC+TGG - Chr4:55282543-55282562 None:intergenic 55.0%
GGATGGGAAAGCGGCAGATA+TGG - Chr4:55283511-55283530 None:intergenic 55.0%
TGTCCAAGAAAGACGGGCAG+CGG - Chr4:55283454-55283473 None:intergenic 55.0%
AGGGACCTCCCTGCTGTGAA+GGG + Chr4:55283650-55283669 MsG0480021218.01.T01:CDS 60.0%
ATGGGACCTTGCGTTGCGGA+AGG + Chr4:55282428-55282447 MsG0480021218.01.T01:CDS 60.0%
GCGTTGCGGAAGGGATGCAT+TGG + Chr4:55282438-55282457 MsG0480021218.01.T01:CDS 60.0%
TGCATCCCTTCCGCAACGCA+AGG - Chr4:55282437-55282456 None:intergenic 60.0%
TGGGACCTTGCGTTGCGGAA+GGG + Chr4:55282429-55282448 MsG0480021218.01.T01:CDS 60.0%
GAGGGACCTCCCTGCTGTGA+AGG + Chr4:55283649-55283668 MsG0480021218.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 55280432 55283947 55280432 ID=MsG0480021218.01;Name=MsG0480021218.01
Chr4 mRNA 55280432 55283947 55280432 ID=MsG0480021218.01.T01;Parent=MsG0480021218.01;Name=MsG0480021218.01.T01;_AED=0.29;_eAED=0.29;_QI=369|0.66|0.75|1|0.66|0.5|4|109|380
Chr4 exon 55280432 55280609 55280432 ID=MsG0480021218.01.T01:exon:6695;Parent=MsG0480021218.01.T01
Chr4 exon 55281893 55282655 55281893 ID=MsG0480021218.01.T01:exon:6696;Parent=MsG0480021218.01.T01
Chr4 exon 55283005 55283159 55283005 ID=MsG0480021218.01.T01:exon:6697;Parent=MsG0480021218.01.T01
Chr4 exon 55283423 55283947 55283423 ID=MsG0480021218.01.T01:exon:6698;Parent=MsG0480021218.01.T01
Chr4 five_prime_UTR 55280432 55280609 55280432 ID=MsG0480021218.01.T01:five_prime_utr;Parent=MsG0480021218.01.T01
Chr4 five_prime_UTR 55281893 55282083 55281893 ID=MsG0480021218.01.T01:five_prime_utr;Parent=MsG0480021218.01.T01
Chr4 CDS 55282084 55282655 55282084 ID=MsG0480021218.01.T01:cds;Parent=MsG0480021218.01.T01
Chr4 CDS 55283005 55283159 55283005 ID=MsG0480021218.01.T01:cds;Parent=MsG0480021218.01.T01
Chr4 CDS 55283423 55283838 55283423 ID=MsG0480021218.01.T01:cds;Parent=MsG0480021218.01.T01
Chr4 three_prime_UTR 55283839 55283947 55283839 ID=MsG0480021218.01.T01:three_prime_utr;Parent=MsG0480021218.01.T01
Gene Sequence

>MsG0480021218.01.T01

ATGGCGGTTCTTACGCGTGTAACTTCAAAACTTGGAGCAAAGTATCGTGCTCTAGCTCCTTTTAGATTTCTAAGGGGTTTGGTGTGTCTATTGGTGCTGGTTTCATCAGCATTTGTAACATTGGTGTTCTTCGGTTTTATTAGTTCTGTTATAATGCGATTTTTTAGCATCAGTTACAGCAGGAAAACAACGTCGTTTTTCTTTGGTACTTGGCTAACTTTGTGGCCCTTTCTATTTGAAAAAATCAACAAGACTAAAGTTGTGTTCTCTGGAGATATTGTTCCTTCGAGAGAACGGATTGTGCTGATCGCAAATCACCGAACTGAAGTCGACTGGATGTATTTATGGGACCTTGCGTTGCGGAAGGGATGCATTGGATACATTAAATATGTACTTAAGAGCAGCTTGATGAGACTTCCAATTTTTGGTTGGGCATTTCACATACTAGAGTTCATCCCAGTGGAAAGGAAGTGGGAAGCTGATGAGTCAAATATGCGTCGAATGCTTTCTACATTCAATGATCCCCAAGATCCTCTCTGGCTTGCTATTTTCCCTGAAGGCACTGATTTTACCCATCTATCATTTTCTTCTTGTAGTGAGCAGAAGTGCCTTCGAAGTCAAAAATATGCGGCTGAACACGGGTTACCGATTCTGAAAAATGTTTTACTGCCGAAAACCAAGGGCTTTTGTACCTGTTTGCAAGAGTTGCGAGGCTCTCTAAATGCAGTTTACGATGTTACCATTGGCTACAAATACCGCTGCCCGTCTTTCTTGGACAATGTATTTGGAGTGGATCCTTCCGAAGTTCATATCCATATCTGCCGCTTTCCCATCCACTGTATCCCAACATCTGAAGATGAAATTTCTACGTGGTTGATGGATAGATTCCGATTTAAGGACAAGTTGCTTTACAATTTTCAATTTCAAGGCCAGTTTCCTGATCAAGCAAAAGAGAGGGACCTCCCTGCTGTGAAGGGCATTTTAAATTGTGTGACAGTGATTATATTGACTGGCTTATGCATGTACTTCACTTTTTCCTCTGTCTGGTTTAAACTTTACGTGTCTGTAGTCGTCGCTTATTTGGTTCCGGCTACATATTTCAACATTCGGCCACAACCCATTCTTGGAGTTTTTAAGATGTGA
Protein sequence

>MsG0480021218.01.T01

MAVLTRVTSKLGAKYRALAPFRFLRGLVCLLVLVSSAFVTLVFFGFISSVIMRFFSISYSRKTTSFFFGTWLTLWPFLFEKINKTKVVFSGDIVPSRERIVLIANHRTEVDWMYLWDLALRKGCIGYIKYVLKSSLMRLPIFGWAFHILEFIPVERKWEADESNMRRMLSTFNDPQDPLWLAIFPEGTDFTHLSFSSCSEQKCLRSQKYAAEHGLPILKNVLLPKTKGFCTCLQELRGSLNAVYDVTIGYKYRCPSFLDNVFGVDPSEVHIHICRFPIHCIPTSEDEISTWLMDRFRFKDKLLYNFQFQGQFPDQAKERDLPAVKGILNCVTVIILTGLCMYFTFSSVWFKLYVSVVVAYLVPATYFNIRPQPILGVFKM*