AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021373.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021373.01.T01 MTR_4g072500 94.565 92 5 0 1 92 1 92 1.00e-61 182
MsG0480021373.01.T01 MTR_4g072530 94.444 90 5 0 1 90 1 90 1.99e-60 179
MsG0480021373.01.T01 MTR_4g072640 89.011 91 10 0 1 91 1 91 2.52e-57 171
MsG0480021373.01.T01 MTR_4g072570 80.435 92 18 0 1 92 1 92 8.96e-51 155
MsG0480021373.01.T01 MTR_4g072310 75.269 93 22 1 1 92 1 93 5.97e-50 152
MsG0480021373.01.T01 MTR_4g072230 77.419 93 18 2 1 92 1 91 2.19e-49 151
MsG0480021373.01.T01 MTR_4g072480 78.261 92 20 0 1 92 1 92 7.15e-49 150
MsG0480021373.01.T01 MTR_4g072680 78.261 92 20 0 1 92 1 92 1.12e-48 149
MsG0480021373.01.T01 MTR_3g084150 73.913 92 24 0 1 92 1 92 1.90e-48 149
MsG0480021373.01.T01 MTR_4g072880 70.968 93 26 1 1 92 1 93 3.74e-48 148
MsG0480021373.01.T01 MTR_4g072730 76.667 90 21 0 1 90 1 90 5.21e-48 147
MsG0480021373.01.T01 MTR_4g072620 76.087 92 22 0 1 92 1 92 8.63e-48 147
MsG0480021373.01.T01 MTR_3g084180 72.826 92 25 0 1 92 1 92 2.10e-47 146
MsG0480021373.01.T01 MTR_8g461410 71.739 92 26 0 1 92 1 92 4.38e-47 145
MsG0480021373.01.T01 MTR_4g072460 76.087 92 22 0 1 92 1 92 6.22e-47 145
MsG0480021373.01.T01 MTR_4g072250 73.118 93 23 2 1 92 1 92 8.01e-47 145
MsG0480021373.01.T01 MTR_4g072860 74.194 93 23 1 1 92 1 93 8.34e-47 145
MsG0480021373.01.T01 MTR_4g072240 70.330 91 26 1 1 90 1 91 9.16e-47 144
MsG0480021373.01.T01 MTR_4g072220 70.968 93 26 1 1 92 1 93 1.78e-46 144
MsG0480021373.01.T01 MTR_4g072270 70.968 93 26 1 1 92 1 93 2.85e-46 143
MsG0480021373.01.T01 MTR_3g117630 74.713 87 22 0 6 92 6 92 6.65e-46 142
MsG0480021373.01.T01 MTR_3g084250 71.739 92 26 0 1 92 1 92 3.16e-45 140
MsG0480021373.01.T01 MTR_4g072870 69.231 91 27 1 1 90 1 91 5.25e-45 140
MsG0480021373.01.T01 MTR_4g050700 70.968 93 26 1 1 92 1 93 7.42e-45 140
MsG0480021373.01.T01 MTR_2g012410 70.968 93 26 1 1 92 1 93 7.42e-45 140
MsG0480021373.01.T01 MTR_4g072300 68.132 91 28 1 1 90 1 91 7.88e-45 139
MsG0480021373.01.T01 MTR_3g084240 73.118 93 23 2 1 92 1 92 2.13e-44 139
MsG0480021373.01.T01 MTR_3g084170 73.118 93 23 2 1 92 1 92 8.68e-44 137
MsG0480021373.01.T01 MTR_3g110042 71.910 89 25 0 1 89 1 89 1.43e-43 137
MsG0480021373.01.T01 MTR_3g110038 72.043 93 24 2 1 92 1 92 1.85e-43 136
MsG0480021373.01.T01 MTR_4g072260 66.667 93 30 1 1 92 1 93 2.27e-43 136
MsG0480021373.01.T01 MTR_4g072330 72.527 91 21 2 1 90 1 88 5.52e-43 135
MsG0480021373.01.T01 MTR_4g072850 72.340 94 24 1 1 92 1 94 8.74e-43 134
MsG0480021373.01.T01 MTR_4g072890 68.132 91 28 1 1 90 1 91 9.21e-43 134
MsG0480021373.01.T01 MTR_3g110032 68.817 93 28 1 1 92 1 93 9.36e-43 134
MsG0480021373.01.T01 MTR_4g072370 72.527 91 21 2 1 90 1 88 1.39e-42 134
MsG0480021373.01.T01 MTR_3g084220 72.043 93 24 2 1 92 1 92 7.89e-42 132
MsG0480021373.01.T01 MTR_4g072660 70.652 92 22 1 1 92 1 87 1.02e-41 132
MsG0480021373.01.T01 MTR_3g084190 69.565 92 28 0 1 92 1 92 2.75e-41 130
MsG0480021373.01.T01 MTR_8g461420 69.565 92 28 0 1 92 1 92 7.15e-41 130
MsG0480021373.01.T01 MTR_3g084260 68.132 91 28 1 1 90 1 91 1.09e-40 129
MsG0480021373.01.T01 MTR_3g084160 67.391 92 30 0 1 92 1 92 2.70e-40 128
MsG0480021373.01.T01 MTR_3g084210 70.652 92 27 0 1 92 1 92 3.14e-40 128
MsG0480021373.01.T01 MTR_4g072830 72.222 90 21 2 1 89 1 87 4.26e-40 127
MsG0480021373.01.T01 MTR_4g072940 77.778 72 16 0 21 92 53 124 9.33e-40 128
MsG0480021373.01.T01 MTR_3g084230 68.478 92 29 0 1 92 1 92 1.34e-39 126
MsG0480021373.01.T01 MTR_5g008360 75.949 79 19 0 14 92 5 83 1.65e-39 126
MsG0480021373.01.T01 MTR_4g072820 68.889 90 23 2 1 90 1 85 2.85e-39 125
MsG0480021373.01.T01 MTR_4g072740 68.132 91 22 2 1 90 1 85 3.97e-39 125
MsG0480021373.01.T01 MTR_4g072470 69.231 91 21 2 1 90 1 85 4.58e-39 125
MsG0480021373.01.T01 MTR_4g072580 67.033 91 23 2 1 90 1 85 7.05e-39 124
MsG0480021373.01.T01 MTR_5g008370 80.822 73 14 0 18 90 18 90 9.65e-39 126
MsG0480021373.01.T01 MTR_3g110045 71.111 90 26 0 3 92 62 151 1.35e-38 126
MsG0480021373.01.T01 MTR_4g072490 64.835 91 25 2 1 90 1 85 3.93e-38 122
MsG0480021373.01.T01 MTR_4g072550 79.167 72 15 0 21 92 32 103 8.97e-38 122
MsG0480021373.01.T01 MTR_4g072610 65.556 90 25 1 1 90 1 84 3.51e-37 120
MsG0480021373.01.T01 MTR_4g072630 64.444 90 28 1 1 90 1 86 3.79e-37 120
MsG0480021373.01.T01 MTR_4g072540 64.444 90 28 1 1 90 1 86 3.79e-37 120
MsG0480021373.01.T01 MTR_4g072600 65.934 91 24 2 1 90 1 85 5.80e-37 120
MsG0480021373.01.T01 MTR_4g072650 65.556 90 25 1 1 90 1 84 1.40e-36 119
MsG0480021373.01.T01 MTR_4g072670 64.444 90 28 1 1 90 1 86 4.01e-36 117
MsG0480021373.01.T01 MTR_4g072720 63.333 90 29 1 1 90 1 86 1.28e-35 116
MsG0480021373.01.T01 MTR_4g072465 60.000 90 32 1 1 90 1 86 1.24e-34 114
MsG0480021373.01.T01 MTR_4g072750 74.324 74 17 1 1 72 1 74 1.45e-34 114
MsG0480021373.01.T01 MTR_4g072590 64.444 90 27 1 1 90 1 85 3.85e-34 112
MsG0480021373.01.T01 MTR_4g072510 61.111 90 31 1 1 90 1 86 4.19e-34 112
MsG0480021373.01.T01 MTR_4g072290 61.290 93 21 2 1 92 1 79 4.09e-33 110
MsG0480021373.01.T01 MTR_4g072690 63.333 90 28 1 1 90 1 85 5.53e-33 109
MsG0480021373.01.T01 MTR_4g072450 63.333 90 28 1 1 90 1 85 6.04e-33 109
MsG0480021373.01.T01 MTR_4g072910 64.865 74 26 0 17 90 27 100 8.86e-33 109
MsG0480021373.01.T01 MTR_4g072380 62.637 91 28 3 1 90 1 86 1.62e-32 108
MsG0480021373.01.T01 MTR_4g072560 54.639 97 36 2 1 89 1 97 2.76e-32 108
MsG0480021373.01.T01 MTR_4g072770 58.242 91 28 1 1 91 1 81 1.49e-31 106
MsG0480021373.01.T01 MTR_4g072980 56.522 92 37 2 1 89 1 92 1.19e-30 104
MsG0480021373.01.T01 MTR_4g072280 64.000 75 26 1 1 74 1 75 4.34e-30 102
MsG0480021373.01.T01 MTR_5g008350 61.250 80 24 2 1 79 1 74 5.80e-30 102
MsG0480021373.01.T01 MTR_3g117640 71.642 67 18 1 25 90 34 100 1.63e-29 101
MsG0480021373.01.T01 MTR_3g084200 67.123 73 22 2 1 72 1 72 1.65e-29 101
MsG0480021373.01.T01 MTR_3g113310 65.152 66 22 1 24 89 41 105 6.98e-28 97.4
MsG0480021373.01.T01 MTR_6g077850 56.522 69 30 0 22 90 31 99 2.44e-27 95.9
MsG0480021373.01.T01 MTR_3g110035 65.152 66 23 0 27 92 73 138 5.10e-27 96.3
MsG0480021373.01.T01 MTR_3g117610 67.188 64 20 1 24 87 44 106 2.89e-26 93.6
MsG0480021373.01.T01 MTR_4g072190 65.152 66 22 1 24 89 35 99 1.08e-25 92.0
MsG0480021373.01.T01 MTR_4g072930 59.420 69 24 1 26 90 42 110 1.95e-23 86.7
MsG0480021373.01.T01 MTR_5g021820 48.837 86 40 2 7 89 19 103 8.92e-23 84.7
MsG0480021373.01.T01 MTR_8g076040 60.938 64 24 1 24 87 40 102 9.89e-23 84.3
MsG0480021373.01.T01 MTR_8g461400 51.613 93 17 3 1 92 1 66 2.16e-22 82.4
MsG0480021373.01.T01 MTR_4g124750 44.318 88 42 3 4 87 46 130 1.36e-18 75.5
MsG0480021373.01.T01 MTR_2g043960 47.887 71 36 1 15 85 66 135 1.78e-18 75.1
MsG0480021373.01.T01 MTR_1g024085 52.941 68 31 1 16 83 28 94 3.85e-18 73.9
MsG0480021373.01.T01 MTR_7g118010 51.471 68 30 2 25 90 23 89 4.98e-18 72.4
MsG0480021373.01.T01 MTR_4g072840 44.444 90 20 2 1 90 1 60 5.23e-18 71.2
MsG0480021373.01.T01 MTR_4g005310 51.515 66 31 1 21 85 58 123 2.88e-17 72.0
MsG0480021373.01.T01 MTR_4g025830 46.479 71 34 2 22 89 76 145 3.98e-17 71.6
MsG0480021373.01.T01 MTR_4g072160 52.174 69 32 1 17 85 29 96 5.10e-17 70.9
MsG0480021373.01.T01 MTR_0388s0020 40.909 88 47 3 5 89 56 141 2.09e-16 69.7
MsG0480021373.01.T01 MTR_3g092220 49.231 65 30 2 21 83 29 92 2.23e-16 68.6
MsG0480021373.01.T01 MTR_8g026730 48.529 68 32 2 25 90 27 93 2.82e-16 68.2
MsG0480021373.01.T01 MTR_1g094750 41.860 86 45 2 10 91 31 115 3.74e-16 68.2
MsG0480021373.01.T01 MTR_1g061570 49.206 63 31 1 23 85 17 78 4.11e-16 68.2
MsG0480021373.01.T01 MTR_8g461210 42.683 82 46 1 4 85 14 94 4.70e-16 67.8
MsG0480021373.01.T01 MTR_1g009150 47.887 71 36 1 15 85 61 130 6.84e-16 68.9
MsG0480021373.01.T01 MTR_1g063950 50.000 60 29 1 23 82 46 104 1.57e-15 66.6
MsG0480021373.01.T01 MTR_7g101740 50.000 64 31 1 24 87 39 101 2.33e-15 65.9
MsG0480021373.01.T01 MTR_1g059770 40.000 70 41 1 20 89 44 112 2.25e-14 63.9
MsG0480021373.01.T01 MTR_4g005320 49.180 61 30 1 25 85 70 129 3.42e-14 64.3
MsG0480021373.01.T01 MTR_3g109160 44.156 77 42 1 9 85 53 128 5.90e-14 63.5
MsG0480021373.01.T01 MTR_3g097120 41.772 79 37 2 21 91 5 82 7.04e-14 62.0
MsG0480021373.01.T01 MTR_2g066830 39.130 92 43 3 2 90 41 122 5.54e-13 60.5
MsG0480021373.01.T01 MTR_8g101520 39.773 88 48 3 8 91 29 115 5.99e-13 60.8
MsG0480021373.01.T01 MTR_7g104940 41.935 62 35 1 28 89 53 113 1.24e-12 59.3
MsG0480021373.01.T01 MTR_7g405740 45.902 61 32 1 25 85 67 126 1.29e-12 60.1
MsG0480021373.01.T01 MTR_8g096470 45.000 60 32 1 26 85 47 105 2.14e-12 58.2
MsG0480021373.01.T01 MTR_3g098970 34.091 88 56 2 5 92 34 119 3.86e-12 58.5
MsG0480021373.01.T01 MTR_7g104950 38.710 62 37 1 28 89 53 113 1.33e-11 56.6
MsG0480021373.01.T01 MTR_8g094980 38.272 81 36 2 24 91 2 81 1.58e-11 55.8
MsG0480021373.01.T01 MTR_3g105900 64.865 37 13 0 23 59 1170 1206 2.70e-11 58.5
MsG0480021373.01.T01 MTR_8g096500 44.828 58 31 1 25 82 49 105 7.03e-11 55.5
MsG0480021373.01.T01 MTR_8g096440 42.623 61 34 1 22 82 42 101 9.75e-11 54.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021373.01.T01 AT4G38840 60.465 86 33 1 6 90 14 99 1.41e-34 114
MsG0480021373.01.T01 AT4G34770 65.476 84 24 2 9 90 22 102 2.51e-32 108
MsG0480021373.01.T01 AT5G18020 62.667 75 27 1 16 89 15 89 3.48e-32 108
MsG0480021373.01.T01 AT5G18060 63.158 76 27 1 16 90 15 90 4.08e-32 107
MsG0480021373.01.T01 AT5G18080 69.118 68 20 1 23 89 22 89 4.55e-32 107
MsG0480021373.01.T01 AT4G38850 64.384 73 23 1 21 90 17 89 1.12e-31 106
MsG0480021373.01.T01 AT5G18050 62.821 78 26 2 13 89 14 89 1.15e-31 106
MsG0480021373.01.T01 AT3G03840 65.278 72 23 1 21 90 23 94 1.41e-31 106
MsG0480021373.01.T01 AT5G18030 65.714 70 23 1 22 90 19 88 1.55e-31 106
MsG0480021373.01.T01 AT2G21200 67.143 70 22 1 21 89 17 86 4.14e-31 105
MsG0480021373.01.T01 AT2G21210 64.706 68 22 1 25 90 28 95 2.73e-30 103
MsG0480021373.01.T01 AT5G18010 54.945 91 39 2 1 90 1 90 3.41e-30 102
MsG0480021373.01.T01 AT4G38825 61.333 75 28 1 17 90 15 89 3.86e-30 102
MsG0480021373.01.T01 AT3G03850 67.606 71 21 1 22 90 22 92 7.16e-30 102
MsG0480021373.01.T01 AT3G03820 63.014 73 24 1 21 90 23 95 9.81e-30 102
MsG0480021373.01.T01 AT2G21210 64.706 68 22 1 25 90 95 162 1.33e-29 103
MsG0480021373.01.T01 AT4G34800 56.471 85 30 2 1 82 1 81 3.59e-28 98.2
MsG0480021373.01.T01 AT3G03830 53.409 88 34 2 10 90 5 92 6.69e-28 97.4
MsG0480021373.01.T01 AT4G34810 68.571 70 17 2 25 89 48 117 8.50e-28 97.8
MsG0480021373.01.T01 AT4G13790 53.488 86 32 3 6 89 12 91 1.23e-26 94.0
MsG0480021373.01.T01 AT4G34780 65.714 70 21 1 25 91 28 97 3.62e-26 93.2
MsG0480021373.01.T01 AT4G34790 66.154 65 20 1 25 87 39 103 1.97e-25 91.7
MsG0480021373.01.T01 AT1G75580 67.692 65 20 1 25 89 44 107 3.64e-25 90.9
MsG0480021373.01.T01 AT4G34760 63.636 66 23 1 24 89 42 106 4.50e-25 90.5
MsG0480021373.01.T01 AT4G38860 59.701 67 27 0 24 90 39 105 9.90e-24 87.0
MsG0480021373.01.T01 AT2G21220 59.091 66 26 1 24 89 39 103 3.57e-23 85.5
MsG0480021373.01.T01 AT2G16580 60.938 64 24 1 24 87 43 105 1.12e-21 82.0
MsG0480021373.01.T01 AT1G19830 62.687 67 23 2 24 89 47 112 2.57e-21 81.3
MsG0480021373.01.T01 AT2G18010 57.812 64 26 1 24 87 47 109 2.27e-20 79.0
MsG0480021373.01.T01 AT4G36110 57.812 64 26 1 24 87 39 101 2.52e-20 78.6
MsG0480021373.01.T01 AT3G43120 47.222 72 37 1 14 85 68 138 5.38e-19 76.6
MsG0480021373.01.T01 AT5G66260 53.030 66 30 1 25 90 34 98 9.01e-19 74.3
MsG0480021373.01.T01 AT3G20220 46.667 75 38 2 15 88 37 110 5.58e-18 72.8
MsG0480021373.01.T01 AT5G20810 53.226 62 28 1 24 85 78 138 1.35e-17 73.2
MsG0480021373.01.T01 AT4G34750 49.231 65 32 1 23 87 35 98 2.51e-17 72.0
MsG0480021373.01.T01 AT4G34750 49.231 65 32 1 23 87 35 98 2.51e-17 72.0
MsG0480021373.01.T01 AT5G20810 53.226 62 28 1 24 85 78 138 3.22e-17 72.8
MsG0480021373.01.T01 AT1G75590 47.945 73 37 1 11 83 28 99 3.70e-17 71.6
MsG0480021373.01.T01 AT3G60690 48.611 72 33 2 21 89 84 154 6.16e-17 71.6
MsG0480021373.01.T01 AT5G10990 47.297 74 37 2 10 83 27 98 7.44e-17 70.9
MsG0480021373.01.T01 AT2G45210 45.833 72 35 2 21 89 75 145 1.26e-16 70.5
MsG0480021373.01.T01 AT3G61900 47.143 70 33 2 24 90 29 97 1.50e-16 69.7
MsG0480021373.01.T01 AT1G19840 55.738 61 26 1 23 83 39 98 1.92e-16 69.7
MsG0480021373.01.T01 AT2G46690 41.558 77 41 2 17 90 16 91 2.86e-16 68.6
MsG0480021373.01.T01 AT3G51200 51.667 60 27 2 24 82 38 96 2.72e-15 65.9
MsG0480021373.01.T01 AT5G53590 46.575 73 33 2 24 91 45 116 3.44e-15 66.2
MsG0480021373.01.T01 AT4G00880 47.826 69 33 2 24 90 27 94 5.69e-15 65.1
MsG0480021373.01.T01 AT4G31320 46.753 77 34 2 9 85 70 139 1.01e-14 66.2
MsG0480021373.01.T01 AT2G36210 42.466 73 42 0 15 87 44 116 1.71e-14 64.3
MsG0480021373.01.T01 AT4G12410 47.059 68 32 2 25 89 76 142 1.76e-14 64.7
MsG0480021373.01.T01 AT2G37030 50.847 59 28 1 24 82 48 105 2.28e-14 63.9
MsG0480021373.01.T01 AT4G22620 43.056 72 37 2 21 89 75 145 2.39e-14 64.7
MsG0480021373.01.T01 AT2G24400 44.578 83 39 2 9 91 54 129 8.36e-14 63.5
MsG0480021373.01.T01 AT3G12830 40.741 81 46 2 10 90 36 114 2.50e-13 61.2
MsG0480021373.01.T01 AT1G56150 42.667 75 42 1 16 90 29 102 8.12e-13 59.3
MsG0480021373.01.T01 AT3G09870 47.143 70 34 2 18 85 38 106 2.20e-12 58.5
MsG0480021373.01.T01 AT1G79130 41.096 73 42 1 17 89 40 111 2.41e-12 58.9
MsG0480021373.01.T01 AT1G76190 49.153 59 28 2 27 85 23 79 2.86e-12 58.5
MsG0480021373.01.T01 AT3G53250 42.373 59 30 1 24 82 51 105 5.00e-12 57.8
MsG0480021373.01.T01 AT3G03847 65.000 40 14 0 43 82 88 127 7.01e-12 57.4
MsG0480021373.01.T01 AT3G53250 42.373 59 30 1 24 82 82 136 7.77e-12 57.8
MsG0480021373.01.T01 AT4G09530 39.437 71 40 2 16 83 19 89 7.90e-12 56.6
MsG0480021373.01.T01 AT1G20470 46.154 65 33 2 21 85 19 81 1.08e-11 57.4
MsG0480021373.01.T01 AT1G16510 42.254 71 40 1 19 89 48 117 1.45e-11 57.0

Find 26 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTGATTTAAGTATGATAT+TGG 0.224179 4:-57198585 None:intergenic
CATCAAGCGGAAGAAGAATT+TGG 0.281625 4:+57198627 MsG0480021373.01.T01:CDS
GTTGATTTAAGTATGATATT+GGG 0.370306 4:-57198584 None:intergenic
GTTTCCGCTTAAATGTTATT+CGG 0.401109 4:+57198460 MsG0480021373.01.T01:CDS
AGAGAATCAGAAGCGGTTTG+TGG 0.491251 4:+57198560 MsG0480021373.01.T01:CDS
TATTCGGAGGGCATCATTCA+CGG 0.507569 4:+57198476 MsG0480021373.01.T01:CDS
TCAAGAATTGCTACATCAAG+CGG 0.512093 4:+57198614 MsG0480021373.01.T01:CDS
TACACGGCAACATAGCCCTT+TGG 0.515785 4:-57198531 None:intergenic
GGCTATGTTGCCGTGTATGT+TGG 0.537170 4:+57198537 MsG0480021373.01.T01:CDS
TCAAGCGAGAAGTTATATGT+TGG 0.546391 4:-57198701 None:intergenic
AGGAATGGTGAGGCCACCCA+TGG 0.554354 4:-57198661 None:intergenic
TCCGCTTAAATGTTATTCGG+AGG 0.563331 4:+57198463 MsG0480021373.01.T01:CDS
CCGCTTAAATGTTATTCGGA+GGG 0.576931 4:+57198464 MsG0480021373.01.T01:CDS
AAAATCAGCAGAAGTTCCAA+AGG 0.579122 4:+57198515 MsG0480021373.01.T01:CDS
CTGATTCTCTCCAACATACA+CGG 0.585067 4:-57198547 None:intergenic
ATTTGGATATGATCATCCCA+TGG 0.585205 4:+57198644 MsG0480021373.01.T01:CDS
TGTAGCAATTCTTGAAACAA+TGG 0.591172 4:-57198606 None:intergenic
GACATCTTCAGTGCAAGGAA+TGG 0.591180 4:-57198676 None:intergenic
AAATCAGCAGAAGTTCCAAA+GGG 0.597255 4:+57198516 MsG0480021373.01.T01:CDS
TGGAAGACATCTTCAGTGCA+AGG 0.619549 4:-57198681 None:intergenic
TTTGGATATGATCATCCCAT+GGG 0.624132 4:+57198645 MsG0480021373.01.T01:CDS
CCCTCCGAATAACATTTAAG+CGG 0.634114 4:-57198464 None:intergenic
GGAATGGTGAGGCCACCCAT+GGG 0.636587 4:-57198660 None:intergenic
GGATATGATCATCCCATGGG+TGG 0.642060 4:+57198648 MsG0480021373.01.T01:CDS
CTTCAGTGCAAGGAATGGTG+AGG 0.671810 4:-57198671 None:intergenic
ATGTTGGAGAGAATCAGAAG+CGG 0.743921 4:+57198553 MsG0480021373.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTGATTTAAGTATGATATT+GGG - Chr4:57198587-57198606 None:intergenic 20.0%
!! GGTTGATTTAAGTATGATAT+TGG - Chr4:57198588-57198607 None:intergenic 25.0%
GTTTCCGCTTAAATGTTATT+CGG + Chr4:57198460-57198479 MsG0480021373.01.T01:CDS 30.0%
TGTAGCAATTCTTGAAACAA+TGG - Chr4:57198609-57198628 None:intergenic 30.0%
AAAATCAGCAGAAGTTCCAA+AGG + Chr4:57198515-57198534 MsG0480021373.01.T01:CDS 35.0%
AAATCAGCAGAAGTTCCAAA+GGG + Chr4:57198516-57198535 MsG0480021373.01.T01:CDS 35.0%
ATTTGGATATGATCATCCCA+TGG + Chr4:57198644-57198663 MsG0480021373.01.T01:CDS 35.0%
TCAAGAATTGCTACATCAAG+CGG + Chr4:57198614-57198633 MsG0480021373.01.T01:CDS 35.0%
TCAAGCGAGAAGTTATATGT+TGG - Chr4:57198704-57198723 None:intergenic 35.0%
TTTGGATATGATCATCCCAT+GGG + Chr4:57198645-57198664 MsG0480021373.01.T01:CDS 35.0%
ATGTTGGAGAGAATCAGAAG+CGG + Chr4:57198553-57198572 MsG0480021373.01.T01:CDS 40.0%
CATCAAGCGGAAGAAGAATT+TGG + Chr4:57198627-57198646 MsG0480021373.01.T01:CDS 40.0%
CCCTCCGAATAACATTTAAG+CGG - Chr4:57198467-57198486 None:intergenic 40.0%
CCGCTTAAATGTTATTCGGA+GGG + Chr4:57198464-57198483 MsG0480021373.01.T01:CDS 40.0%
CTGATTCTCTCCAACATACA+CGG - Chr4:57198550-57198569 None:intergenic 40.0%
TCCGCTTAAATGTTATTCGG+AGG + Chr4:57198463-57198482 MsG0480021373.01.T01:CDS 40.0%
!!! TGCTGATTTTAGAGCTGCTT+GGG - Chr4:57198505-57198524 None:intergenic 40.0%
AGAGAATCAGAAGCGGTTTG+TGG + Chr4:57198560-57198579 MsG0480021373.01.T01:CDS 45.0%
GACATCTTCAGTGCAAGGAA+TGG - Chr4:57198679-57198698 None:intergenic 45.0%
TGGAAGACATCTTCAGTGCA+AGG - Chr4:57198684-57198703 None:intergenic 45.0%
!! TATTCGGAGGGCATCATTCA+CGG + Chr4:57198476-57198495 MsG0480021373.01.T01:CDS 45.0%
!!! CTGCTGATTTTAGAGCTGCT+TGG - Chr4:57198506-57198525 None:intergenic 45.0%
CTTCAGTGCAAGGAATGGTG+AGG - Chr4:57198674-57198693 None:intergenic 50.0%
GGATATGATCATCCCATGGG+TGG + Chr4:57198648-57198667 MsG0480021373.01.T01:CDS 50.0%
GGCTATGTTGCCGTGTATGT+TGG + Chr4:57198537-57198556 MsG0480021373.01.T01:CDS 50.0%
TACACGGCAACATAGCCCTT+TGG - Chr4:57198534-57198553 None:intergenic 50.0%
AGGAATGGTGAGGCCACCCA+TGG - Chr4:57198664-57198683 None:intergenic 60.0%
GGAATGGTGAGGCCACCCAT+GGG - Chr4:57198663-57198682 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 57198456 57198734 57198456 ID=MsG0480021373.01;Name=MsG0480021373.01
Chr4 mRNA 57198456 57198734 57198456 ID=MsG0480021373.01.T01;Parent=MsG0480021373.01;Name=MsG0480021373.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|92
Chr4 exon 57198456 57198734 57198456 ID=MsG0480021373.01.T01:exon:7184;Parent=MsG0480021373.01.T01
Chr4 CDS 57198456 57198734 57198456 ID=MsG0480021373.01.T01:cds;Parent=MsG0480021373.01.T01
Gene Sequence

>MsG0480021373.01.T01

ATGGGTTTCCGCTTAAATGTTATTCGGAGGGCATCATTCACGGCTACCCAAGCAGCTCTAAAATCAGCAGAAGTTCCAAAGGGCTATGTTGCCGTGTATGTTGGAGAGAATCAGAAGCGGTTTGTGGTCCCAATATCATACTTAAATCAACCATTGTTTCAAGAATTGCTACATCAAGCGGAAGAAGAATTTGGATATGATCATCCCATGGGTGGCCTCACCATTCCTTGCACTGAAGATGTCTTCCAACATATAACTTCTCGCTTGAATGAGCTATGA
Protein sequence

>MsG0480021373.01.T01

MGFRLNVIRRASFTATQAALKSAEVPKGYVAVYVGENQKRFVVPISYLNQPLFQELLHQAEEEFGYDHPMGGLTIPCTEDVFQHITSRLNEL*