AlfalfaGEDB | MsG0480021375.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021375.01

KEGG: K14488
GO: GO:0009733
PFAM: PF02519

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021375.01.T01	AES89338.1	95.652	92	4	0	1	92	1	92	1.08E-58	184

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021375.01.T01	P33083	76.667	90	21	0	1	90	1	90	4.42E-47	148

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021375.01.T01	G7JM95	95.652	92	4	0	1	92	1	92	5.18e-59	184

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180002279.01	MsG0480021375.01	0.805264	1.451544e-49	7.644927e-47
MsG0480020783.01	MsG0480021375.01	0.804828	1.790781e-49	9.327403e-47
MsG0480021375.01	MsG0680034064.01	0.801829	7.484353e-49	3.609881e-46
MsG0480021375.01	MsG0780038948.01	0.808415	3.132260e-50	1.789888e-47
MsG0380016192.01	MsG0480021375.01	0.809409	1.919584e-50	1.126217e-47

PPI

Gene1	Gene2	Type
MsG0480021386.01	MsG0480021375.01	PPI
MsG0480021386.01	MsG0480021375.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021375.01.T01	MTR_4g072680	95.652	92	4	0	1	92	1	92	1.31e-62	184
MsG0480021375.01.T01	MTR_4g072460	93.478	92	6	0	1	92	1	92	4.41e-61	181
MsG0480021375.01.T01	MTR_4g072480	92.391	92	7	0	1	92	1	92	1.06e-60	180
MsG0480021375.01.T01	MTR_4g072620	89.130	92	10	0	1	92	1	92	3.41e-59	176
MsG0480021375.01.T01	MTR_4g072730	85.714	91	13	0	1	91	1	91	3.02e-56	168
MsG0480021375.01.T01	MTR_4g072660	80.435	92	13	1	1	92	1	87	1.56e-50	154
MsG0480021375.01.T01	MTR_4g072570	79.348	92	19	0	1	92	1	92	2.09e-50	154
MsG0480021375.01.T01	MTR_4g072500	78.261	92	20	0	1	92	1	92	1.91e-49	151
MsG0480021375.01.T01	MTR_4g072530	76.667	90	21	0	1	90	1	90	4.31e-47	145
MsG0480021375.01.T01	MTR_4g072640	74.444	90	23	0	1	90	1	90	3.64e-46	143
MsG0480021375.01.T01	MTR_4g072310	70.968	93	26	1	1	92	1	93	4.77e-46	143
MsG0480021375.01.T01	MTR_3g084150	70.652	92	27	0	1	92	1	92	5.06e-46	142
MsG0480021375.01.T01	MTR_8g461410	69.565	92	28	0	1	92	1	92	2.38e-45	141
MsG0480021375.01.T01	MTR_3g084180	69.565	92	28	0	1	92	1	92	5.07e-45	140
MsG0480021375.01.T01	MTR_4g072230	72.043	93	23	2	1	92	1	91	6.98e-45	140
MsG0480021375.01.T01	MTR_4g072880	68.817	93	28	1	1	92	1	93	8.10e-45	140
MsG0480021375.01.T01	MTR_4g072270	69.892	93	27	1	1	92	1	93	3.48e-44	138
MsG0480021375.01.T01	MTR_4g072250	72.043	93	24	2	1	92	1	92	2.84e-43	135
MsG0480021375.01.T01	MTR_3g084240	67.391	92	30	0	1	92	1	92	2.97e-43	135
MsG0480021375.01.T01	MTR_3g084170	67.391	92	30	0	1	92	1	92	1.08e-42	134
MsG0480021375.01.T01	MTR_4g072860	69.892	93	27	1	1	92	1	93	1.29e-42	134
MsG0480021375.01.T01	MTR_5g008360	78.750	80	17	0	13	92	4	83	2.81e-42	133
MsG0480021375.01.T01	MTR_3g117630	71.264	87	25	0	6	92	6	92	6.33e-42	132
MsG0480021375.01.T01	MTR_3g110042	70.787	89	26	0	1	89	1	89	6.55e-42	132
MsG0480021375.01.T01	MTR_4g072240	64.835	91	31	1	1	90	1	91	9.02e-42	132
MsG0480021375.01.T01	MTR_3g084220	66.304	92	31	0	1	92	1	92	7.23e-41	130
MsG0480021375.01.T01	MTR_3g084250	66.304	92	31	0	1	92	1	92	8.81e-41	129
MsG0480021375.01.T01	MTR_4g072870	64.835	91	31	1	1	90	1	91	1.20e-40	129
MsG0480021375.01.T01	MTR_4g072550	82.192	73	13	0	20	92	31	103	2.75e-40	129
MsG0480021375.01.T01	MTR_4g072220	62.366	93	34	1	1	92	1	93	2.87e-40	128
MsG0480021375.01.T01	MTR_4g050700	65.591	93	31	1	1	92	1	93	5.08e-40	127
MsG0480021375.01.T01	MTR_2g012410	65.591	93	31	1	1	92	1	93	5.08e-40	127
MsG0480021375.01.T01	MTR_4g072300	61.538	91	34	1	1	90	1	91	7.10e-40	127
MsG0480021375.01.T01	MTR_3g084210	67.391	92	30	0	1	92	1	92	1.56e-39	126
MsG0480021375.01.T01	MTR_4g072370	65.556	90	29	1	1	90	1	88	2.25e-39	126
MsG0480021375.01.T01	MTR_3g110038	63.043	92	34	0	1	92	1	92	3.04e-39	125
MsG0480021375.01.T01	MTR_4g072740	67.778	90	24	1	1	90	1	85	3.64e-39	125
MsG0480021375.01.T01	MTR_4g072490	65.556	90	26	1	1	90	1	85	3.68e-39	125
MsG0480021375.01.T01	MTR_4g072630	65.556	90	27	1	1	90	1	86	4.72e-39	125
MsG0480021375.01.T01	MTR_3g110032	63.441	93	33	1	1	92	1	93	6.53e-39	125
MsG0480021375.01.T01	MTR_4g072330	63.333	90	31	1	1	90	1	88	1.01e-38	124
MsG0480021375.01.T01	MTR_4g072890	61.538	91	34	1	1	90	1	91	3.37e-38	123
MsG0480021375.01.T01	MTR_4g072850	67.021	94	29	1	1	92	1	94	3.41e-38	123
MsG0480021375.01.T01	MTR_4g072260	60.215	93	36	1	1	92	1	93	3.70e-38	123
MsG0480021375.01.T01	MTR_4g072580	63.333	90	28	1	1	90	1	85	4.81e-38	122
MsG0480021375.01.T01	MTR_3g084190	66.304	92	31	0	1	92	1	92	1.19e-37	121
MsG0480021375.01.T01	MTR_8g461420	66.667	90	30	0	1	90	1	90	1.24e-37	121
MsG0480021375.01.T01	MTR_5g008370	75.676	74	18	0	18	91	18	91	2.96e-37	122
MsG0480021375.01.T01	MTR_3g084230	66.667	90	30	0	1	90	1	90	3.04e-37	120
MsG0480021375.01.T01	MTR_3g084260	61.290	93	35	1	1	92	1	93	3.74e-37	120
MsG0480021375.01.T01	MTR_4g072470	64.444	90	27	1	1	90	1	85	4.44e-37	120
MsG0480021375.01.T01	MTR_4g072600	64.444	90	27	1	1	90	1	85	4.76e-37	120
MsG0480021375.01.T01	MTR_4g072830	64.045	89	30	1	1	89	1	87	9.08e-37	119
MsG0480021375.01.T01	MTR_3g084160	65.217	92	32	0	1	92	1	92	4.57e-36	117
MsG0480021375.01.T01	MTR_4g072465	60.000	90	32	1	1	90	1	86	9.33e-36	117
MsG0480021375.01.T01	MTR_4g072610	64.444	90	26	1	1	90	1	84	1.17e-35	116
MsG0480021375.01.T01	MTR_3g110045	66.667	90	30	0	3	92	62	151	1.27e-35	118
MsG0480021375.01.T01	MTR_4g072540	64.444	90	28	1	1	90	1	86	1.48e-35	116
MsG0480021375.01.T01	MTR_4g072720	64.444	90	28	1	1	90	1	86	1.08e-34	114
MsG0480021375.01.T01	MTR_4g072940	72.222	72	20	0	21	92	53	124	1.56e-34	115
MsG0480021375.01.T01	MTR_4g072820	62.637	91	27	2	1	90	1	85	4.62e-34	112
MsG0480021375.01.T01	MTR_4g072670	62.222	90	30	1	1	90	1	86	3.08e-33	110
MsG0480021375.01.T01	MTR_4g072650	60.000	90	30	1	1	90	1	84	4.57e-33	110
MsG0480021375.01.T01	MTR_4g072510	63.333	90	29	1	1	90	1	86	5.51e-33	109
MsG0480021375.01.T01	MTR_4g072590	63.333	90	28	1	1	90	1	85	5.65e-33	109
MsG0480021375.01.T01	MTR_4g072380	60.440	91	30	2	1	90	1	86	1.64e-32	108
MsG0480021375.01.T01	MTR_4g072290	56.989	93	25	2	1	92	1	79	1.82e-31	105
MsG0480021375.01.T01	MTR_4g072770	58.889	90	27	1	1	90	1	80	2.91e-31	105
MsG0480021375.01.T01	MTR_5g008350	60.759	79	26	1	1	79	1	74	1.92e-30	103
MsG0480021375.01.T01	MTR_4g072690	61.111	90	30	1	1	90	1	85	9.43e-30	101
MsG0480021375.01.T01	MTR_4g072750	66.216	74	23	1	1	72	1	74	1.53e-29	101
MsG0480021375.01.T01	MTR_4g072450	61.111	90	30	1	1	90	1	85	2.05e-29	100
MsG0480021375.01.T01	MTR_3g084200	62.500	72	27	0	1	72	1	72	2.79e-29	100
MsG0480021375.01.T01	MTR_4g072910	56.627	83	35	1	9	90	18	100	1.11e-28	99.4
MsG0480021375.01.T01	MTR_4g072980	51.613	93	39	2	1	92	8	95	1.18e-28	99.4
MsG0480021375.01.T01	MTR_4g072560	54.217	83	37	1	8	89	15	97	6.12e-28	97.4
MsG0480021375.01.T01	MTR_3g117640	64.706	68	23	1	25	91	34	101	8.90e-27	94.7
MsG0480021375.01.T01	MTR_3g110035	62.687	67	25	0	26	92	72	138	1.25e-26	95.5
MsG0480021375.01.T01	MTR_6g077850	55.224	67	30	0	24	90	33	99	9.33e-26	92.0
MsG0480021375.01.T01	MTR_4g072280	54.667	75	33	1	1	74	1	75	1.69e-24	88.2
MsG0480021375.01.T01	MTR_3g113310	55.224	67	29	1	23	89	40	105	1.62e-23	86.7
MsG0480021375.01.T01	MTR_3g117610	58.462	65	26	1	23	87	43	106	3.94e-22	83.2
MsG0480021375.01.T01	MTR_4g072190	53.521	71	32	1	19	89	30	99	4.21e-22	82.8
MsG0480021375.01.T01	MTR_8g461400	45.652	92	24	1	1	92	1	66	7.54e-20	76.3
MsG0480021375.01.T01	MTR_4g072930	51.471	68	29	1	27	90	43	110	2.11e-19	76.3
MsG0480021375.01.T01	MTR_8g076040	53.125	64	29	1	24	87	40	102	1.47e-18	73.9
MsG0480021375.01.T01	MTR_5g021820	48.485	66	33	1	24	89	39	103	2.39e-18	73.2
MsG0480021375.01.T01	MTR_4g124750	42.045	88	48	2	4	89	46	132	8.00e-18	73.6
MsG0480021375.01.T01	MTR_2g043960	45.205	73	39	1	17	89	68	139	8.90e-17	70.9
MsG0480021375.01.T01	MTR_4g072840	42.857	91	20	3	1	90	1	60	7.66e-16	65.9
MsG0480021375.01.T01	MTR_4g005310	46.269	67	35	1	21	86	58	124	2.20e-15	67.0
MsG0480021375.01.T01	MTR_1g024085	44.186	86	44	2	4	86	13	97	2.57e-15	66.6
MsG0480021375.01.T01	MTR_8g461210	39.535	86	51	1	4	89	14	98	3.73e-15	65.5
MsG0480021375.01.T01	MTR_1g009150	51.613	62	29	1	25	86	71	131	6.48e-15	66.2
MsG0480021375.01.T01	MTR_3g109160	43.750	80	44	1	12	91	56	134	9.54e-15	65.9
MsG0480021375.01.T01	MTR_4g072160	48.611	72	34	2	16	86	28	97	3.47e-14	63.5
MsG0480021375.01.T01	MTR_1g061570	47.059	68	35	1	24	91	18	84	4.39e-14	63.2
MsG0480021375.01.T01	MTR_7g118010	52.381	63	27	2	25	85	23	84	7.33e-14	62.0
MsG0480021375.01.T01	MTR_0388s0020	44.156	77	39	2	12	85	62	137	1.11e-13	62.4
MsG0480021375.01.T01	MTR_1g094750	46.154	65	34	1	24	88	49	112	1.23e-13	61.6
MsG0480021375.01.T01	MTR_4g005320	46.032	63	33	1	25	87	70	131	1.33e-13	62.8
MsG0480021375.01.T01	MTR_3g092220	40.541	74	41	2	12	83	20	92	2.32e-13	60.8
MsG0480021375.01.T01	MTR_4g025830	45.588	68	33	2	25	89	79	145	3.92e-13	61.2
MsG0480021375.01.T01	MTR_8g026730	49.206	63	29	2	25	85	27	88	7.41e-13	59.3
MsG0480021375.01.T01	MTR_2g066830	38.272	81	49	1	10	90	43	122	1.48e-12	59.3
MsG0480021375.01.T01	MTR_8g101520	38.636	88	49	2	7	90	28	114	3.90e-12	58.9
MsG0480021375.01.T01	MTR_1g059770	41.935	62	35	1	28	89	52	112	4.09e-12	58.2
MsG0480021375.01.T01	MTR_1g063310	37.079	89	51	3	5	90	6	92	7.13e-12	56.6
MsG0480021375.01.T01	MTR_7g104940	36.620	71	44	1	19	89	44	113	9.15e-12	57.0
MsG0480021375.01.T01	MTR_4g021810	46.875	64	32	2	25	87	26	88	1.09e-11	56.2
MsG0480021375.01.T01	MTR_3g098970	33.333	78	51	1	15	92	43	119	1.16e-11	57.4
MsG0480021375.01.T01	MTR_7g104950	36.000	75	46	2	16	89	40	113	1.52e-11	56.6
MsG0480021375.01.T01	MTR_3g097120	40.541	74	35	2	20	85	4	76	1.58e-11	55.8
MsG0480021375.01.T01	MTR_7g405740	40.625	64	37	1	27	90	69	131	4.28e-11	56.2
MsG0480021375.01.T01	MTR_7g101740	42.188	64	36	1	24	87	39	101	5.52e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021375.01.T01	AT2G21200	58.824	85	33	2	7	89	2	86	1.48e-30	103
MsG0480021375.01.T01	AT4G38840	54.545	99	36	3	1	90	1	99	2.11e-30	103
MsG0480021375.01.T01	AT4G34770	56.667	90	37	1	4	91	14	103	9.07e-30	102
MsG0480021375.01.T01	AT4G34800	53.846	91	35	2	1	88	1	87	1.47e-29	101
MsG0480021375.01.T01	AT2G21210	50.526	95	42	2	1	90	1	95	8.78e-29	99.8
MsG0480021375.01.T01	AT3G03840	54.945	91	36	2	3	91	8	95	3.74e-28	97.8
MsG0480021375.01.T01	AT4G38825	52.874	87	37	2	8	90	3	89	4.33e-28	97.8
MsG0480021375.01.T01	AT2G21210	50.526	95	42	2	1	90	68	162	5.42e-28	99.8
MsG0480021375.01.T01	AT5G18080	52.747	91	41	2	1	90	1	90	6.33e-28	97.4
MsG0480021375.01.T01	AT5G18060	51.685	89	38	2	7	90	2	90	6.91e-28	97.1
MsG0480021375.01.T01	AT5G18020	52.273	88	40	2	1	87	1	87	7.76e-28	97.1
MsG0480021375.01.T01	AT3G03850	58.242	91	31	3	3	91	8	93	2.83e-27	95.9
MsG0480021375.01.T01	AT5G18030	51.724	87	39	2	7	90	2	88	4.79e-27	95.1
MsG0480021375.01.T01	AT3G03820	55.056	89	34	2	6	91	11	96	5.08e-27	95.1
MsG0480021375.01.T01	AT4G34810	63.235	68	21	1	25	88	48	115	6.00e-27	95.5
MsG0480021375.01.T01	AT5G18050	50.562	89	39	2	7	90	2	90	6.52e-27	94.7
MsG0480021375.01.T01	AT4G38850	53.409	88	37	2	7	90	2	89	1.41e-26	94.0
MsG0480021375.01.T01	AT5G18010	49.451	91	44	2	1	90	1	90	7.90e-26	92.0
MsG0480021375.01.T01	AT4G13790	48.352	91	45	1	1	89	1	91	1.04e-25	91.7
MsG0480021375.01.T01	AT3G03830	50.549	91	36	2	3	90	8	92	1.21e-23	86.7
MsG0480021375.01.T01	AT4G34790	59.091	66	25	1	25	88	39	104	8.18e-23	84.7
MsG0480021375.01.T01	AT4G34780	59.155	71	26	1	23	90	26	96	1.48e-22	84.3
MsG0480021375.01.T01	AT1G75580	50.000	80	39	1	10	89	29	107	1.21e-21	82.0
MsG0480021375.01.T01	AT4G38860	54.412	68	31	0	23	90	38	105	2.04e-21	81.3
MsG0480021375.01.T01	AT2G21220	55.224	67	29	1	23	89	38	103	2.72e-21	80.9
MsG0480021375.01.T01	AT4G34760	55.224	67	29	1	23	89	41	106	2.76e-21	80.9
MsG0480021375.01.T01	AT1G19830	52.857	70	32	1	20	89	43	111	2.91e-20	78.6
MsG0480021375.01.T01	AT4G36110	54.688	64	28	1	24	87	39	101	1.07e-18	74.3
MsG0480021375.01.T01	AT2G16580	50.746	67	32	1	23	89	42	107	5.05e-18	72.8
MsG0480021375.01.T01	AT2G18010	53.125	64	29	1	24	87	47	109	9.95e-18	72.0
MsG0480021375.01.T01	AT5G66260	53.030	66	30	1	25	90	34	98	1.64e-17	71.2
MsG0480021375.01.T01	AT3G43120	42.308	78	44	1	14	91	68	144	2.19e-17	72.4
MsG0480021375.01.T01	AT3G20220	46.753	77	35	3	11	86	37	108	9.39e-17	69.7
MsG0480021375.01.T01	AT5G20810	45.205	73	39	1	17	89	71	142	3.56e-16	69.3
MsG0480021375.01.T01	AT5G20810	45.205	73	39	1	17	89	71	142	3.86e-16	69.7
MsG0480021375.01.T01	AT3G12830	43.678	87	47	2	4	90	30	114	1.22e-15	67.4
MsG0480021375.01.T01	AT3G51200	43.939	66	36	1	26	91	41	105	2.31e-15	65.9
MsG0480021375.01.T01	AT1G75590	47.727	88	40	3	4	86	16	102	2.82e-15	67.0
MsG0480021375.01.T01	AT5G10990	52.381	63	29	1	24	86	40	101	1.38e-14	65.1
MsG0480021375.01.T01	AT4G34750	48.438	64	32	1	24	87	36	98	1.45e-14	65.1
MsG0480021375.01.T01	AT4G34750	48.438	64	32	1	24	87	36	98	1.45e-14	65.1
MsG0480021375.01.T01	AT1G19840	45.977	87	42	2	4	86	16	101	2.66e-14	64.3
MsG0480021375.01.T01	AT3G09870	48.485	66	31	2	24	87	44	108	1.43e-13	61.6
MsG0480021375.01.T01	AT2G46690	40.506	79	43	2	16	91	15	92	1.60e-13	61.6
MsG0480021375.01.T01	AT5G53590	49.254	67	28	2	24	85	45	110	3.01e-13	61.2
MsG0480021375.01.T01	AT3G61900	45.714	70	34	2	24	90	29	97	4.32e-13	60.8
MsG0480021375.01.T01	AT1G56150	43.678	87	44	3	7	90	18	102	9.35e-13	59.3
MsG0480021375.01.T01	AT2G45210	39.726	73	40	2	20	89	74	145	1.46e-12	60.1
MsG0480021375.01.T01	AT3G03847	40.741	81	45	3	7	86	53	131	1.59e-12	59.3
MsG0480021375.01.T01	AT3G60690	45.588	68	33	2	21	85	84	150	1.72e-12	60.1
MsG0480021375.01.T01	AT4G00880	48.438	64	30	2	24	85	27	89	1.55e-11	56.6
MsG0480021375.01.T01	AT4G09530	41.176	68	37	2	27	91	30	97	2.21e-11	55.5
MsG0480021375.01.T01	AT4G31320	45.161	62	33	1	25	86	80	140	2.70e-11	57.0
MsG0480021375.01.T01	AT2G37030	41.270	63	36	1	24	86	48	109	5.04e-11	55.1
MsG0480021375.01.T01	AT2G24400	46.774	62	32	1	25	86	64	124	5.25e-11	56.2
MsG0480021375.01.T01	AT1G16510	43.077	65	36	1	25	89	54	117	5.61e-11	55.5

Find 26 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATATAATTTCTTCCTTGAA+TGG	0.329759	4:+57208705	MsG0480021375.01.T01:CDS
CTCAACAAGTCTTGAAATGA+AGG	0.342172	4:-57208606	None:intergenic
AGTCAAGCCGAGGAAGAGTT+TGG	0.367159	4:+57208627	MsG0480021375.01.T01:CDS
CTCTTAGAATAGAGTTTAAA+CGG	0.400936	4:-57208464	None:intergenic
GGTTTGTTCAAGTATGATAC+AGG	0.410295	4:-57208585	None:intergenic
CGTTTAAACTCTATTCTAAG+AGG	0.413979	4:+57208465	MsG0480021375.01.T01:CDS
CGTGTATGTTGGAGAGAAAC+TGG	0.417120	4:+57208548	MsG0480021375.01.T01:CDS
TAAGATTCATAGTCCATTCA+AGG	0.465375	4:-57208718	None:intergenic
AAAATCAGTAGAAGTGAGAA+AGG	0.471464	4:+57208515	MsG0480021375.01.T01:CDS
GGATATGATCATCCCATTGG+TGG	0.489018	4:+57208648	MsG0480021375.01.T01:CDS
TGGCCTCACCATTCCTTGTA+CGG	0.498841	4:+57208668	MsG0480021375.01.T01:CDS
TGGAAGACATCTTCCGTACA+AGG	0.532292	4:-57208681	None:intergenic
ATCATATCCAAACTCTTCCT+CGG	0.532636	4:-57208634	None:intergenic
GGCTATGTTGCCGTGTATGT+TGG	0.537170	4:+57208537	MsG0480021375.01.T01:CDS
GACATCTTCCGTACAAGGAA+TGG	0.540589	4:-57208676	None:intergenic
TCAAGGAAGAAATTATATGT+TGG	0.547755	4:-57208701	None:intergenic
TTTGGATATGATCATCCCAT+TGG	0.549842	4:+57208645	MsG0480021375.01.T01:CDS
AAACTCTATTCTAAGAGGAT+CGG	0.556160	4:+57208470	MsG0480021375.01.T01:CDS
GGAATGGTGAGGCCACCAAT+GGG	0.576067	4:-57208660	None:intergenic
AGGAATGGTGAGGCCACCAA+TGG	0.576442	4:-57208661	None:intergenic
AAATCAGTAGAAGTGAGAAA+GGG	0.578349	4:+57208516	MsG0480021375.01.T01:CDS
CAGTTTCTCTCCAACATACA+CGG	0.594831	4:-57208547	None:intergenic
AGACTTGTTGAGTCAAGCCG+AGG	0.633253	4:+57208617	MsG0480021375.01.T01:CDS
CTTCCGTACAAGGAATGGTG+AGG	0.686019	4:-57208671	None:intergenic
GTTTGTTCAAGTATGATACA+GGG	0.688837	4:-57208584	None:intergenic
ATGTTGGAGAGAAACTGGCG+CGG	0.774668	4:+57208553	MsG0480021375.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CATATAATTTCTTCCTTGAA+TGG	+	Chr4:57208705-57208724	MsG0480021375.01.T01:CDS	25.0%
!	CTCTTAGAATAGAGTTTAAA+CGG	-	Chr4:57208467-57208486	None:intergenic	25.0%
!	TCAAGGAAGAAATTATATGT+TGG	-	Chr4:57208704-57208723	None:intergenic	25.0%
	AAAATCAGTAGAAGTGAGAA+AGG	+	Chr4:57208515-57208534	MsG0480021375.01.T01:CDS	30.0%
	AAATCAGTAGAAGTGAGAAA+GGG	+	Chr4:57208516-57208535	MsG0480021375.01.T01:CDS	30.0%
	CGTTTAAACTCTATTCTAAG+AGG	+	Chr4:57208465-57208484	MsG0480021375.01.T01:CDS	30.0%
	GTTTGTTCAAGTATGATACA+GGG	-	Chr4:57208587-57208606	None:intergenic	30.0%
!	AAACTCTATTCTAAGAGGAT+CGG	+	Chr4:57208470-57208489	MsG0480021375.01.T01:CDS	30.0%
	ATCATATCCAAACTCTTCCT+CGG	-	Chr4:57208637-57208656	None:intergenic	35.0%
	CTCAACAAGTCTTGAAATGA+AGG	-	Chr4:57208609-57208628	None:intergenic	35.0%
	TTTGGATATGATCATCCCAT+TGG	+	Chr4:57208645-57208664	MsG0480021375.01.T01:CDS	35.0%
!	GGTTTGTTCAAGTATGATAC+AGG	-	Chr4:57208588-57208607	None:intergenic	35.0%
	CAGTTTCTCTCCAACATACA+CGG	-	Chr4:57208550-57208569	None:intergenic	40.0%
	CGTGTATGTTGGAGAGAAAC+TGG	+	Chr4:57208548-57208567	MsG0480021375.01.T01:CDS	45.0%
	GACATCTTCCGTACAAGGAA+TGG	-	Chr4:57208679-57208698	None:intergenic	45.0%
	GGATATGATCATCCCATTGG+TGG	+	Chr4:57208648-57208667	MsG0480021375.01.T01:CDS	45.0%
	TGGAAGACATCTTCCGTACA+AGG	-	Chr4:57208684-57208703	None:intergenic	45.0%
	AGTCAAGCCGAGGAAGAGTT+TGG	+	Chr4:57208627-57208646	MsG0480021375.01.T01:CDS	50.0%
	ATGTTGGAGAGAAACTGGCG+CGG	+	Chr4:57208553-57208572	MsG0480021375.01.T01:CDS	50.0%
	CTTCCGTACAAGGAATGGTG+AGG	-	Chr4:57208674-57208693	None:intergenic	50.0%
	GGCTATGTTGCCGTGTATGT+TGG	+	Chr4:57208537-57208556	MsG0480021375.01.T01:CDS	50.0%
	TGGCCTCACCATTCCTTGTA+CGG	+	Chr4:57208668-57208687	MsG0480021375.01.T01:CDS	50.0%
!!	AGACTTGTTGAGTCAAGCCG+AGG	+	Chr4:57208617-57208636	MsG0480021375.01.T01:CDS	50.0%
	AGGAATGGTGAGGCCACCAA+TGG	-	Chr4:57208664-57208683	None:intergenic	55.0%
	GGAATGGTGAGGCCACCAAT+GGG	-	Chr4:57208663-57208682	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	57208456	57208734	57208456	ID=MsG0480021375.01;Name=MsG0480021375.01
Chr4	mRNA	57208456	57208734	57208456	ID=MsG0480021375.01.T01;Parent=MsG0480021375.01;Name=MsG0480021375.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|92
Chr4	exon	57208456	57208734	57208456	ID=MsG0480021375.01.T01:exon:7185;Parent=MsG0480021375.01.T01
Chr4	CDS	57208456	57208734	57208456	ID=MsG0480021375.01.T01:cds;Parent=MsG0480021375.01.T01

Gene Sequence	>MsG0480021375.01.T01 ATGGGTTTCCGTTTAAACTCTATTCTAAGAGGATCGGTAACAGCTAGACAAGCAACATCAAAATCAGTAGAAGTGAGAAAGGGCTATGTTGCCGTGTATGTTGGAGAGAAACTGGCGCGGTTTGTAGTCCCTGTATCATACTTGAACAAACCTTCATTTCAAGACTTGTTGAGTCAAGCCGAGGAAGAGTTTGGATATGATCATCCCATTGGTGGCCTCACCATTCCTTGTACGGAAGATGTCTTCCAACATATAATTTCTTCCTTGAATGGACTATGA
Protein sequence	>MsG0480021375.01.T01 MGFRLNSILRGSVTARQATSKSVEVRKGYVAVYVGEKLARFVVPVSYLNKPSFQDLLSQAEEEFGYDHPIGGLTIPCTEDVFQHIISSLNGL*