Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021407.01.T01 | XP_024630855.2 | 56.8 | 125 | 46 | 2 | 10 | 132 | 121 | 239 | 3.68E-27 | 111 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021407.01.T01 | A0A072VP56 | 56.800 | 125 | 46 | 2 | 10 | 132 | 229 | 347 | 9.54e-27 | 112 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004426.01 | MsG0480021407.01 | 0.804311 | 2.295476e-49 | 1.179701e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021407.01.T01 | MTR_1g492690 | 56.800 | 125 | 46 | 2 | 10 | 132 | 229 | 347 | 2.42e-30 | 112 |
| MsG0480021407.01.T01 | MTR_1g052715 | 50.575 | 87 | 43 | 0 | 4 | 90 | 240 | 326 | 7.90e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGAAAGATTATCGAGTTT+TGG | 0.287971 | 4:-57583593 | MsG0480021407.01.T01:three_prime_UTR |
| GACTCCAACTAGCAAATCTT+TGG | 0.288947 | 4:-57583726 | MsG0480021407.01.T01:CDS |
| GTTTCCGTTAATGATTGTTT+TGG | 0.312248 | 4:-57583426 | MsG0480021407.01.T01:three_prime_UTR |
| CGAAGTTGCTGCTGCTGATT+CGG | 0.386996 | 4:-57583909 | MsG0480021407.01.T01:CDS |
| AATTCCAAAACAATCATTAA+CGG | 0.403566 | 4:+57583422 | None:intergenic |
| GCCTAAGCTTGTGCGTGTTA+AGG | 0.403824 | 4:-57583638 | MsG0480021407.01.T01:exon |
| GACTCCAACTAGCAAACCTT+TGG | 0.409498 | 4:-57583882 | MsG0480021407.01.T01:CDS |
| TGCGGCAACATCATCTTTGT+TGG | 0.415154 | 4:+57583763 | None:intergenic |
| TTTCCCAAAGATTTGCTAGT+TGG | 0.458073 | 4:+57583722 | None:intergenic |
| ACTCCAACTAGCAAATCTTT+GGG | 0.491743 | 4:-57583725 | MsG0480021407.01.T01:CDS |
| GTTGTTGTGCATAGTTCTGA+TGG | 0.499773 | 4:-57583821 | MsG0480021407.01.T01:CDS |
| TTTCCCAAAGGTTTGCTAGT+TGG | 0.503027 | 4:+57583878 | None:intergenic |
| AAATGAAAGGACAACAGACT+TGG | 0.519997 | 4:+57583555 | None:intergenic |
| ACTCCAACTAGCAAACCTTT+GGG | 0.520423 | 4:-57583881 | MsG0480021407.01.T01:CDS |
| TGCTACAAAAGAGCTAAAGC+TGG | 0.538283 | 4:-57583795 | MsG0480021407.01.T01:CDS |
| ACCTTAACACGCACAAGCTT+AGG | 0.546106 | 4:+57583637 | None:intergenic |
| TTTCAAGAGACTTCACTCAT+CGG | 0.565680 | 4:+57583609 | None:intergenic |
| CTAGCAAACCTTTGGGAAAG+CGG | 0.578566 | 4:-57583874 | MsG0480021407.01.T01:CDS |
| TCAGTAGACCGCTTTCCCAA+AGG | 0.587701 | 4:+57583866 | None:intergenic |
| AAAGCGGTCTACTGATAGTG+TGG | 0.622760 | 4:-57583858 | MsG0480021407.01.T01:CDS |
| CATGCAGGAAAATATTGAAG+TGG | 0.661188 | 4:-57583951 | MsG0480021407.01.T01:intron |
| GTTAGCTTATCAAAATCCTG+TGG | 0.715142 | 4:-57584057 | MsG0480021407.01.T01:intron |
| AGTCACAACTGAATCAACTG+CGG | 0.772555 | 4:+57583745 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAACAAAACAAAATGAA+AGG | + | Chr4:57584018-57584037 | None:intergenic | 15.0% |
| ! | ATCATTTCTGCAGTTTATAA+AGG | - | Chr4:57583535-57583554 | MsG0480021407.01.T01:three_prime_UTR | 25.0% |
| ! | GCTCAAAAAAAGAAATCAAA+AGG | + | Chr4:57583434-57583453 | None:intergenic | 25.0% |
| ! | TGATCAAAAAAATATACCAC+AGG | + | Chr4:57583519-57583538 | None:intergenic | 25.0% |
| !!! | TTGATTTCTTTTTTTGAGCT+TGG | - | Chr4:57583435-57583454 | MsG0480021407.01.T01:three_prime_UTR | 25.0% |
| ! | CTTGAAAGATTATCGAGTTT+TGG | - | Chr4:57583964-57583983 | MsG0480021407.01.T01:intron | 30.0% |
| ! | GTTTCCGTTAATGATTGTTT+TGG | - | Chr4:57584131-57584150 | MsG0480021407.01.T01:CDS | 30.0% |
| AAATGAAAGGACAACAGACT+TGG | + | Chr4:57584005-57584024 | None:intergenic | 35.0% | |
| ACTCCAACTAGCAAATCTTT+GGG | - | Chr4:57583832-57583851 | MsG0480021407.01.T01:CDS | 35.0% | |
| CATGCAGGAAAATATTGAAG+TGG | - | Chr4:57583606-57583625 | MsG0480021407.01.T01:three_prime_UTR | 35.0% | |
| GCATAAAAACACCATAAGTC+TGG | + | Chr4:57584085-57584104 | None:intergenic | 35.0% | |
| TTTCAAGAGACTTCACTCAT+CGG | + | Chr4:57583951-57583970 | None:intergenic | 35.0% | |
| ! | GTTAGCTTATCAAAATCCTG+TGG | - | Chr4:57583500-57583519 | MsG0480021407.01.T01:three_prime_UTR | 35.0% |
| !! | TTTCCCAAAGATTTGCTAGT+TGG | + | Chr4:57583838-57583857 | None:intergenic | 35.0% |
| ACTCCAACTAGCAAACCTTT+GGG | - | Chr4:57583676-57583695 | MsG0480021407.01.T01:CDS | 40.0% | |
| AGTCACAACTGAATCAACTG+CGG | + | Chr4:57583815-57583834 | None:intergenic | 40.0% | |
| GACTCCAACTAGCAAATCTT+TGG | - | Chr4:57583831-57583850 | MsG0480021407.01.T01:CDS | 40.0% | |
| TGCTACAAAAGAGCTAAAGC+TGG | - | Chr4:57583762-57583781 | MsG0480021407.01.T01:CDS | 40.0% | |
| !! | ATCACTTTTCTCATGCATGC+AGG | - | Chr4:57583591-57583610 | MsG0480021407.01.T01:three_prime_UTR | 40.0% |
| !! | GTTGTTGTGCATAGTTCTGA+TGG | - | Chr4:57583736-57583755 | MsG0480021407.01.T01:CDS | 40.0% |
| !! | TTTCCCAAAGGTTTGCTAGT+TGG | + | Chr4:57583682-57583701 | None:intergenic | 40.0% |
| ACCTTAACACGCACAAGCTT+AGG | + | Chr4:57583923-57583942 | None:intergenic | 45.0% | |
| GACTCCAACTAGCAAACCTT+TGG | - | Chr4:57583675-57583694 | MsG0480021407.01.T01:CDS | 45.0% | |
| TGCGGCAACATCATCTTTGT+TGG | + | Chr4:57583797-57583816 | None:intergenic | 45.0% | |
| ! | AAAGCGGTCTACTGATAGTG+TGG | - | Chr4:57583699-57583718 | MsG0480021407.01.T01:CDS | 45.0% |
| ! | CTAGCAAACCTTTGGGAAAG+CGG | - | Chr4:57583683-57583702 | MsG0480021407.01.T01:CDS | 45.0% |
| ! | TTTTGCTGCTGCCAGACTTA+TGG | - | Chr4:57584071-57584090 | MsG0480021407.01.T01:CDS | 45.0% |
| !!! | TTTTGAGCTTGGTAACGACG+AGG | - | Chr4:57583446-57583465 | MsG0480021407.01.T01:three_prime_UTR | 45.0% |
| GCCTAAGCTTGTGCGTGTTA+AGG | - | Chr4:57583919-57583938 | MsG0480021407.01.T01:CDS | 50.0% | |
| TCAGTAGACCGCTTTCCCAA+AGG | + | Chr4:57583694-57583713 | None:intergenic | 50.0% | |
| ! | CGAAGTTGCTGCTGCTGATT+CGG | - | Chr4:57583648-57583667 | MsG0480021407.01.T01:exon | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 57583426 | 57584153 | 57583426 | ID=MsG0480021407.01;Name=MsG0480021407.01 |
| Chr4 | mRNA | 57583426 | 57584153 | 57583426 | ID=MsG0480021407.01.T01;Parent=MsG0480021407.01;Name=MsG0480021407.01.T01;_AED=0.34;_eAED=0.34;_QI=0|1|1|1|0.11|0.2|10|229|135 |
| Chr4 | exon | 57583426 | 57583966 | 57583426 | ID=MsG0480021407.01.T01:exon:19320;Parent=MsG0480021407.01.T01 |
| Chr4 | exon | 57584058 | 57584153 | 57584058 | ID=MsG0480021407.01.T01:exon:19319;Parent=MsG0480021407.01.T01 |
| Chr4 | CDS | 57584058 | 57584153 | 57584058 | ID=MsG0480021407.01.T01:cds;Parent=MsG0480021407.01.T01 |
| Chr4 | CDS | 57583655 | 57583966 | 57583655 | ID=MsG0480021407.01.T01:cds;Parent=MsG0480021407.01.T01 |
| Chr4 | three_prime_UTR | 57583426 | 57583654 | 57583426 | ID=MsG0480021407.01.T01:three_prime_utr;Parent=MsG0480021407.01.T01 |
| Gene Sequence |
| Protein sequence |
