AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004426.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 8 sgRNAs with CRISPR-Local

Find 7 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCTCAGCAGTAATGAGTT+TGG 0.430118 1:+77761849 MsG0180004426.01.T01:CDS
TTGAAGAAGAGACAAGAAGA+AGG 0.558667 1:+77761759 MsG0180004426.01.T01:CDS
TGTACTCTTTGAGCACCACC+AGG 0.609997 1:-77761824 None:intergenic
GATGAAGATGATGAGATGCC+TGG 0.615030 1:+77761806 MsG0180004426.01.T01:CDS
AAGGCAGCAAGCTCAGCAAG+AGG 0.624868 1:+77761778 MsG0180004426.01.T01:CDS
ATGAGTTTGGAGATGTGCTG+TGG 0.657658 1:+77761862 MsG0180004426.01.T01:CDS
CATGGAGCTGGATGAGTGTG+AGG 0.667513 1:+77761712 None:intergenic
GAAGATGATGAGATGCCTGG+TGG 0.783796 1:+77761809 MsG0180004426.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTGAAGAAGAGACAAGAAGA+AGG + Chr1:77761759-77761778 MsG0180004426.01.T01:CDS 35.0%
ATGAGTTTGGAGATGTGCTG+TGG + Chr1:77761862-77761881 MsG0180004426.01.T01:CDS 45.0%
GATGAAGATGATGAGATGCC+TGG + Chr1:77761806-77761825 MsG0180004426.01.T01:CDS 45.0%
GTGCTCAGCAGTAATGAGTT+TGG + Chr1:77761849-77761868 MsG0180004426.01.T01:CDS 45.0%
GAAGATGATGAGATGCCTGG+TGG + Chr1:77761809-77761828 MsG0180004426.01.T01:CDS 50.0%
TGTACTCTTTGAGCACCACC+AGG - Chr1:77761827-77761846 None:intergenic 50.0%
AAGGCAGCAAGCTCAGCAAG+AGG + Chr1:77761778-77761797 MsG0180004426.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 77761723 77761887 77761723 ID=MsG0180004426.01;Name=MsG0180004426.01
Chr1 mRNA 77761723 77761887 77761723 ID=MsG0180004426.01.T01;Parent=MsG0180004426.01;Name=MsG0180004426.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|54
Chr1 exon 77761723 77761887 77761723 ID=MsG0180004426.01.T01:exon:7357;Parent=MsG0180004426.01.T01
Chr1 CDS 77761723 77761887 77761723 ID=MsG0180004426.01.T01:cds;Parent=MsG0180004426.01.T01
Gene Sequence

>MsG0180004426.01.T01

ATGAGTGTGAGGAGACAACACTTCATGACGTCAACATTGAAGAAGAGACAAGAAGAAGGCAGCAAGCTCAGCAAGAGGCATATGATGAAGATGATGAGATGCCTGGTGGTGCTCAAAGAGTACAGTGTGCTCAGCAGTAATGAGTTTGGAGATGTGCTGTGGTAG

Protein sequence

>MsG0180004426.01.T01

MSVRRQHFMTSTLKKRQEEGSKLSKRHMMKMMRCLVVLKEYSVLSSNEFGDVLW*