Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021511.01.T01 | AES89438.2 | 100 | 138 | 0 | 0 | 14 | 151 | 197 | 334 | 3.70E-91 | 278 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021511.01.T01 | P80030 | 83.333 | 132 | 22 | 0 | 14 | 145 | 253 | 384 | 1.10E-71 | 223 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021511.01.T01 | G7JNJ7 | 100.000 | 138 | 0 | 0 | 14 | 151 | 197 | 334 | 1.77e-91 | 278 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480021510.01 | MsG0480021511.01 | 0.820657 | 6.108835e-53 | 4.862625e-50 |
| MsG0480021511.01 | MsG0480021719.01 | 0.859006 | 5.572474e-63 | 1.449997e-59 |
| MsG0380016352.01 | MsG0480021511.01 | 0.804502 | 2.094751e-49 | 1.081786e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021511.01.T01 | MTR_4g074950 | 100.000 | 138 | 0 | 0 | 14 | 151 | 197 | 334 | 4.48e-95 | 278 |
| MsG0480021511.01.T01 | MTR_7g053290 | 89.855 | 138 | 14 | 0 | 14 | 151 | 255 | 392 | 1.08e-85 | 256 |
| MsG0480021511.01.T01 | MTR_2g066850 | 79.104 | 67 | 14 | 0 | 14 | 80 | 964 | 1030 | 8.72e-29 | 110 |
| MsG0480021511.01.T01 | MTR_7g037650 | 79.245 | 53 | 11 | 0 | 36 | 88 | 464 | 516 | 1.72e-20 | 87.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021511.01.T01 | AT2G05990 | 76.923 | 143 | 33 | 0 | 3 | 145 | 245 | 387 | 4.56e-72 | 221 |
| MsG0480021511.01.T01 | AT2G05990 | 76.923 | 143 | 33 | 0 | 3 | 145 | 245 | 387 | 4.56e-72 | 221 |
Find 43 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAAGCAATGTACAGAATT+TGG | 0.164025 | 4:-58878463 | None:intergenic |
| GTCTAAGTTGGGGTGAGATT+TGG | 0.271766 | 4:-58878252 | None:intergenic |
| TCTAAGTTGGGGTGAGATTT+GGG | 0.319052 | 4:-58878251 | None:intergenic |
| GGGACTTAATGCTGCTCCTT+GGG | 0.385473 | 4:-58878231 | None:intergenic |
| GAAAGAAATTAATGCCTAGT+AGG | 0.404777 | 4:+58878490 | MsG0480021511.01.T01:three_prime_UTR |
| CAGTCCTATATTTAGTGATC+TGG | 0.407629 | 4:+58878203 | MsG0480021511.01.T01:CDS |
| TTTGCAGCACGACTTCTTAG+TGG | 0.421625 | 4:-58877902 | None:intergenic |
| AGTCGTGCTGCAAAAGCAAT+TGG | 0.429867 | 4:+58877911 | MsG0480021511.01.T01:CDS |
| TGGGACTTAATGCTGCTCCT+TGG | 0.447749 | 4:-58878232 | None:intergenic |
| ATGTCCAGATCACTAAATAT+AGG | 0.451168 | 4:-58878207 | None:intergenic |
| TGCTATATGCTCAGAAGAGC+AGG | 0.451770 | 4:+58878369 | MsG0480021511.01.T01:three_prime_UTR |
| GAGACTACACTGTCTAAGTT+GGG | 0.456735 | 4:-58878263 | None:intergenic |
| CTCTTTCTGTAGAGGTGCAT+TGG | 0.459409 | 4:-58877966 | None:intergenic |
| TACCTGTCTGACGTATTTGT+AGG | 0.461717 | 4:+58877316 | MsG0480021511.01.T01:CDS |
| TTCATATGTAACAGAGGAGG+TGG | 0.465333 | 4:+58878080 | MsG0480021511.01.T01:intron |
| AGCGAAGCAAATACTCTAAG+TGG | 0.478194 | 4:-58878285 | None:intergenic |
| GGAGACTACACTGTCTAAGT+TGG | 0.491103 | 4:-58878264 | None:intergenic |
| AAAGAAATTAATGCCTAGTA+GGG | 0.491481 | 4:+58878491 | MsG0480021511.01.T01:three_prime_UTR |
| GCATCAGAGAGGATCATTCC+TGG | 0.496552 | 4:+58877367 | MsG0480021511.01.T01:CDS |
| CTTGTCGTCACCTTTAGCAT+CGG | 0.500580 | 4:+58878119 | MsG0480021511.01.T01:CDS |
| TGACAATGGTTTGAATGCTA+TGG | 0.518772 | 4:+58878167 | MsG0480021511.01.T01:CDS |
| AAAGTCTCTACCACCCTACT+AGG | 0.520612 | 4:-58878504 | None:intergenic |
| CATATAGCAGATACATAGAA+AGG | 0.540587 | 4:-58878355 | None:intergenic |
| GACAATGGTTTGAATGCTAT+GGG | 0.544764 | 4:+58878168 | MsG0480021511.01.T01:CDS |
| CTACAGAAAGAGCTATCAGC+AGG | 0.561168 | 4:+58877977 | MsG0480021511.01.T01:CDS |
| AGAGTCAACACTATATCTGC+TGG | 0.562777 | 4:+58877664 | MsG0480021511.01.T01:CDS |
| GGTTTGAATGCTATGGGCGT+TGG | 0.565599 | 4:+58878174 | MsG0480021511.01.T01:CDS |
| CACCTACAAATACGTCAGAC+AGG | 0.570930 | 4:-58877318 | None:intergenic |
| TGGTCTAGATTGCATTACTG+TGG | 0.573217 | 4:+58878437 | MsG0480021511.01.T01:three_prime_UTR |
| GCTGATAGCTCTTTCTGTAG+AGG | 0.574157 | 4:-58877974 | None:intergenic |
| CTGTCTGACGTATTTGTAGG+TGG | 0.577586 | 4:+58877319 | MsG0480021511.01.T01:CDS |
| CATCAGAGAGGATCATTCCT+GGG | 0.580610 | 4:+58877368 | MsG0480021511.01.T01:CDS |
| CCTTTAGCATCGGCTATCAC+AGG | 0.582392 | 4:+58878129 | MsG0480021511.01.T01:CDS |
| TAGTGATCTGGACATTCCCA+AGG | 0.589605 | 4:+58878215 | MsG0480021511.01.T01:CDS |
| ACCGTTCTATATGTTGACAA+TGG | 0.601081 | 4:+58878153 | MsG0480021511.01.T01:CDS |
| ACCATTGTCAACATATAGAA+CGG | 0.609256 | 4:-58878154 | None:intergenic |
| CCTGTGATAGCCGATGCTAA+AGG | 0.610330 | 4:-58878129 | None:intergenic |
| GAAATTAATGCCTAGTAGGG+TGG | 0.614070 | 4:+58878494 | MsG0480021511.01.T01:three_prime_UTR |
| TCATATGTAACAGAGGAGGT+GGG | 0.636128 | 4:+58878081 | MsG0480021511.01.T01:intron |
| TGATGATACAGATACGGCGG+TGG | 0.642111 | 4:+58877467 | MsG0480021511.01.T01:intron |
| AGACTACACTGTCTAAGTTG+GGG | 0.666950 | 4:-58878262 | None:intergenic |
| TCACATACATCGCATCAGAG+AGG | 0.710453 | 4:+58877356 | MsG0480021511.01.T01:CDS |
| AAATGATGATACAGATACGG+CGG | 0.727109 | 4:+58877464 | MsG0480021511.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATAAAAATGTTTGATCTA+TGG | + | Chr4:58878409-58878428 | MsG0480021511.01.T01:three_prime_UTR | 15.0% |
| ! | AAAGAAATTAATGCCTAGTA+GGG | + | Chr4:58878491-58878510 | MsG0480021511.01.T01:three_prime_UTR | 25.0% |
| ! | AACTAAAAATAACACTGAGA+CGG | - | Chr4:58877549-58877568 | None:intergenic | 25.0% |
| ! | ACTAAATGATGATACAGATA+CGG | + | Chr4:58877461-58877480 | MsG0480021511.01.T01:intron | 25.0% |
| ! | TGCAAATAATCAAAAGATTG+AGG | - | Chr4:58878046-58878065 | None:intergenic | 25.0% |
| !! | ACTTCATTTGATTTAAACGT+AGG | - | Chr4:58877729-58877748 | None:intergenic | 25.0% |
| !! | TCAATTTTCATATGTAACAG+AGG | + | Chr4:58878074-58878093 | MsG0480021511.01.T01:intron | 25.0% |
| !!! | ATGTTTGATCTATGGTTTTA+TGG | + | Chr4:58878417-58878436 | MsG0480021511.01.T01:three_prime_UTR | 25.0% |
| !!! | TCAGTGTTATTTTTAGTTGT+TGG | + | Chr4:58877551-58877570 | MsG0480021511.01.T01:intron | 25.0% |
| AACTTCAACCAAGATGAAAT+TGG | - | Chr4:58877815-58877834 | None:intergenic | 30.0% | |
| AATTTAAAAATCCCAGTGAC+AGG | - | Chr4:58877584-58877603 | None:intergenic | 30.0% | |
| ACCATTGTCAACATATAGAA+CGG | - | Chr4:58878157-58878176 | None:intergenic | 30.0% | |
| ATGTCCAGATCACTAAATAT+AGG | - | Chr4:58878210-58878229 | None:intergenic | 30.0% | |
| ATTTAAAAATCCCAGTGACA+GGG | - | Chr4:58877583-58877602 | None:intergenic | 30.0% | |
| CATATAGCAGATACATAGAA+AGG | - | Chr4:58878358-58878377 | None:intergenic | 30.0% | |
| GAAAGAAATTAATGCCTAGT+AGG | + | Chr4:58878490-58878509 | MsG0480021511.01.T01:three_prime_UTR | 30.0% | |
| TGATAAAAAGAGAAGTCACA+AGG | - | Chr4:58877692-58877711 | None:intergenic | 30.0% | |
| TTCAAGCAATGTACAGAATT+TGG | - | Chr4:58878466-58878485 | None:intergenic | 30.0% | |
| ! | ATTTTCATATGTAACAGAGG+AGG | + | Chr4:58878077-58878096 | MsG0480021511.01.T01:intron | 30.0% |
| ! | CATTTGATTTAAACGTAGGA+AGG | - | Chr4:58877725-58877744 | None:intergenic | 30.0% |
| !!! | AGCTTAATTCATGCTTTTTC+AGG | + | Chr4:58877606-58877625 | MsG0480021511.01.T01:intron | 30.0% |
| !!! | TATTTGCTTCGCTTTTTTGT+AGG | + | Chr4:58878295-58878314 | MsG0480021511.01.T01:three_prime_UTR | 30.0% |
| AAATGATGATACAGATACGG+CGG | + | Chr4:58877464-58877483 | MsG0480021511.01.T01:intron | 35.0% | |
| ACCAAGATGAAATTGGTAGA+AGG | - | Chr4:58877808-58877827 | None:intergenic | 35.0% | |
| ACCGTTCTATATGTTGACAA+TGG | + | Chr4:58878153-58878172 | MsG0480021511.01.T01:CDS | 35.0% | |
| ATCAAAAGATTGAGGTGAAC+TGG | - | Chr4:58878038-58878057 | None:intergenic | 35.0% | |
| CAAGATGAAATTGGTAGAAG+GGG | - | Chr4:58877806-58877825 | None:intergenic | 35.0% | |
| CAGTCCTATATTTAGTGATC+TGG | + | Chr4:58878203-58878222 | MsG0480021511.01.T01:CDS | 35.0% | |
| CCAAGATGAAATTGGTAGAA+GGG | - | Chr4:58877807-58877826 | None:intergenic | 35.0% | |
| GACAATGGTTTGAATGCTAT+GGG | + | Chr4:58878168-58878187 | MsG0480021511.01.T01:CDS | 35.0% | |
| TAAGCATAATCAACATACCC+AGG | - | Chr4:58877388-58877407 | None:intergenic | 35.0% | |
| TGACAATGGTTTGAATGCTA+TGG | + | Chr4:58878167-58878186 | MsG0480021511.01.T01:CDS | 35.0% | |
| !! | GTTCGTGAATTTAGTTCTTG+TGG | + | Chr4:58877853-58877872 | MsG0480021511.01.T01:intron | 35.0% |
| AGACTACACTGTCTAAGTTG+GGG | - | Chr4:58878265-58878284 | None:intergenic | 40.0% | |
| AGAGTCAACACTATATCTGC+TGG | + | Chr4:58877664-58877683 | MsG0480021511.01.T01:CDS | 40.0% | |
| AGCAAATACGAAACAGTACG+AGG | - | Chr4:58877780-58877799 | None:intergenic | 40.0% | |
| AGCGAAGCAAATACTCTAAG+TGG | - | Chr4:58878288-58878307 | None:intergenic | 40.0% | |
| CCCTTCTACCAATTTCATCT+TGG | + | Chr4:58877804-58877823 | MsG0480021511.01.T01:intron | 40.0% | |
| GAAATTAATGCCTAGTAGGG+TGG | + | Chr4:58878494-58878513 | MsG0480021511.01.T01:three_prime_UTR | 40.0% | |
| GAGACTACACTGTCTAAGTT+GGG | - | Chr4:58878266-58878285 | None:intergenic | 40.0% | |
| GTTACAAGGAACAATCCTGA+TGG | - | Chr4:58878011-58878030 | None:intergenic | 40.0% | |
| TACCTGTCTGACGTATTTGT+AGG | + | Chr4:58877316-58877335 | MsG0480021511.01.T01:CDS | 40.0% | |
| TCATATGTAACAGAGGAGGT+GGG | + | Chr4:58878081-58878100 | MsG0480021511.01.T01:intron | 40.0% | |
| TGGTCTAGATTGCATTACTG+TGG | + | Chr4:58878437-58878456 | MsG0480021511.01.T01:three_prime_UTR | 40.0% | |
| TTCATATGTAACAGAGGAGG+TGG | + | Chr4:58878080-58878099 | MsG0480021511.01.T01:intron | 40.0% | |
| ! | TCTAAGTTGGGGTGAGATTT+GGG | - | Chr4:58878254-58878273 | None:intergenic | 40.0% |
| AAAGTCTCTACCACCCTACT+AGG | - | Chr4:58878507-58878526 | None:intergenic | 45.0% | |
| AGTCGTGCTGCAAAAGCAAT+TGG | + | Chr4:58877911-58877930 | MsG0480021511.01.T01:CDS | 45.0% | |
| CACCTACAAATACGTCAGAC+AGG | - | Chr4:58877321-58877340 | None:intergenic | 45.0% | |
| CAGCAGGTAACATTTCCATC+AGG | + | Chr4:58877993-58878012 | MsG0480021511.01.T01:intron | 45.0% | |
| CATCAGAGAGGATCATTCCT+GGG | + | Chr4:58877368-58877387 | MsG0480021511.01.T01:CDS | 45.0% | |
| CTACAGAAAGAGCTATCAGC+AGG | + | Chr4:58877977-58877996 | MsG0480021511.01.T01:CDS | 45.0% | |
| CTGTCTGACGTATTTGTAGG+TGG | + | Chr4:58877319-58877338 | MsG0480021511.01.T01:CDS | 45.0% | |
| CTTGTCGTCACCTTTAGCAT+CGG | + | Chr4:58878119-58878138 | MsG0480021511.01.T01:CDS | 45.0% | |
| GCTGATAGCTCTTTCTGTAG+AGG | - | Chr4:58877977-58877996 | None:intergenic | 45.0% | |
| GGAGACTACACTGTCTAAGT+TGG | - | Chr4:58878267-58878286 | None:intergenic | 45.0% | |
| GGTGAACTGGTTGTGTTACA+AGG | - | Chr4:58878025-58878044 | None:intergenic | 45.0% | |
| TAGTGATCTGGACATTCCCA+AGG | + | Chr4:58878215-58878234 | MsG0480021511.01.T01:CDS | 45.0% | |
| TCACATACATCGCATCAGAG+AGG | + | Chr4:58877356-58877375 | MsG0480021511.01.T01:CDS | 45.0% | |
| TGCTATATGCTCAGAAGAGC+AGG | + | Chr4:58878369-58878388 | MsG0480021511.01.T01:three_prime_UTR | 45.0% | |
| TTTGCAGCACGACTTCTTAG+TGG | - | Chr4:58877905-58877924 | None:intergenic | 45.0% | |
| ! | CTCTTTCTGTAGAGGTGCAT+TGG | - | Chr4:58877969-58877988 | None:intergenic | 45.0% |
| ! | GTCTAAGTTGGGGTGAGATT+TGG | - | Chr4:58878255-58878274 | None:intergenic | 45.0% |
| AACTCGTGTCTCTTGCTTCC+AGG | + | Chr4:58877878-58877897 | MsG0480021511.01.T01:intron | 50.0% | |
| CCTGTGATAGCCGATGCTAA+AGG | - | Chr4:58878132-58878151 | None:intergenic | 50.0% | |
| GCATCAGAGAGGATCATTCC+TGG | + | Chr4:58877367-58877386 | MsG0480021511.01.T01:CDS | 50.0% | |
| GGGACTTAATGCTGCTCCTT+GGG | - | Chr4:58878234-58878253 | None:intergenic | 50.0% | |
| GGTTTGAATGCTATGGGCGT+TGG | + | Chr4:58878174-58878193 | MsG0480021511.01.T01:CDS | 50.0% | |
| TGATGATACAGATACGGCGG+TGG | + | Chr4:58877467-58877486 | MsG0480021511.01.T01:intron | 50.0% | |
| TGGGACTTAATGCTGCTCCT+TGG | - | Chr4:58878235-58878254 | None:intergenic | 50.0% | |
| TTGGCTTCTTCCCTGTCACT+GGG | + | Chr4:58877570-58877589 | MsG0480021511.01.T01:intron | 50.0% | |
| !! | CCTTTAGCATCGGCTATCAC+AGG | + | Chr4:58878129-58878148 | MsG0480021511.01.T01:CDS | 50.0% |
| !!! | CAGGTGCTTGCTTTTGAAGC+TGG | + | Chr4:58877625-58877644 | MsG0480021511.01.T01:intron | 50.0% |
| ! | GCACGACTTCTTAGTGGACC+TGG | - | Chr4:58877899-58877918 | None:intergenic | 55.0% |
| ! | GTTGGCTTCTTCCCTGTCAC+TGG | + | Chr4:58877569-58877588 | MsG0480021511.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 58877298 | 58878527 | 58877298 | ID=MsG0480021511.01;Name=MsG0480021511.01 |
| Chr4 | mRNA | 58877298 | 58878527 | 58877298 | ID=MsG0480021511.01.T01;Parent=MsG0480021511.01;Name=MsG0480021511.01.T01;_AED=0.44;_eAED=0.44;_QI=0|1|0.8|1|1|1|5|279|151 |
| Chr4 | exon | 58877298 | 58877389 | 58877298 | ID=MsG0480021511.01.T01:exon:17379;Parent=MsG0480021511.01.T01 |
| Chr4 | exon | 58877478 | 58877529 | 58877478 | ID=MsG0480021511.01.T01:exon:17380;Parent=MsG0480021511.01.T01 |
| Chr4 | exon | 58877628 | 58877685 | 58877628 | ID=MsG0480021511.01.T01:exon:17381;Parent=MsG0480021511.01.T01 |
| Chr4 | exon | 58877900 | 58877998 | 58877900 | ID=MsG0480021511.01.T01:exon:17382;Parent=MsG0480021511.01.T01 |
| Chr4 | exon | 58878094 | 58878527 | 58878094 | ID=MsG0480021511.01.T01:exon:17383;Parent=MsG0480021511.01.T01 |
| Chr4 | CDS | 58877298 | 58877389 | 58877298 | ID=MsG0480021511.01.T01:cds;Parent=MsG0480021511.01.T01 |
| Chr4 | CDS | 58877478 | 58877529 | 58877478 | ID=MsG0480021511.01.T01:cds;Parent=MsG0480021511.01.T01 |
| Chr4 | CDS | 58877628 | 58877685 | 58877628 | ID=MsG0480021511.01.T01:cds;Parent=MsG0480021511.01.T01 |
| Chr4 | CDS | 58877900 | 58877998 | 58877900 | ID=MsG0480021511.01.T01:cds;Parent=MsG0480021511.01.T01 |
| Chr4 | CDS | 58878094 | 58878248 | 58878094 | ID=MsG0480021511.01.T01:cds;Parent=MsG0480021511.01.T01 |
| Chr4 | three_prime_UTR | 58878249 | 58878527 | 58878249 | ID=MsG0480021511.01.T01:three_prime_utr;Parent=MsG0480021511.01.T01 |
| Gene Sequence |
| Protein sequence |
