AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021517.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021517.01.T01 MTR_4g029740 79.592 98 20 0 43 140 109 206 3.29e-52 169
MsG0480021517.01.T01 MTR_2g026970 70.312 64 19 0 139 202 3 66 1.00e-23 93.2
MsG0480021517.01.T01 MTR_3g463980 79.310 58 12 0 145 202 73 130 5.00e-21 85.9
MsG0480021517.01.T01 MTR_3g005890 81.250 48 9 0 157 204 18 65 2.04e-15 70.1
MsG0480021517.01.T01 MTR_1g077860 52.381 63 29 1 131 193 51 112 2.52e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTACCCGATACATTGTATTT+TGG 0.212367 4:-58984572 None:intergenic
GTCAGACAACTTCATCTTCT+TGG 0.215009 4:+58985537 MsG0480021517.01.T01:CDS
GACAAGAAAGGGCGCATTTA+TGG 0.248271 4:+58985577 MsG0480021517.01.T01:CDS
AGATTATGCACGTCTGTATC+AGG 0.275262 4:+58982529 MsG0480021517.01.T01:CDS
AAAATTGGGAAGATAGTAAT+TGG 0.276288 4:+58984652 MsG0480021517.01.T01:CDS
TCAGGGACTTGGGCAACTTA+TGG 0.306287 4:+58982547 MsG0480021517.01.T01:CDS
ACTTGTGTATAACGAGTTTC+AGG 0.307971 4:+58982599 MsG0480021517.01.T01:CDS
GAAAGGGCGCATTTATGGTT+TGG 0.368062 4:+58985582 MsG0480021517.01.T01:CDS
CAGGGACTTGGGCAACTTAT+GGG 0.372142 4:+58982548 MsG0480021517.01.T01:CDS
TTCATCTTCTTGGACATAGT+TGG 0.409670 4:+58985547 MsG0480021517.01.T01:CDS
CTCATATGCTTATATTCATA+TGG 0.418784 4:-58985661 None:intergenic
AATGAAAACTTATCATCATA+AGG 0.456645 4:+58984536 MsG0480021517.01.T01:CDS
GCACGTCTGTATCAGGGACT+TGG 0.456884 4:+58982536 MsG0480021517.01.T01:CDS
TGAGATGCCGTCAGATGATC+TGG 0.458884 4:-58985638 None:intergenic
CATATGAATATAAGCATATG+AGG 0.468136 4:+58985662 MsG0480021517.01.T01:CDS
ATCTTCTTGGACATAGTTGG+AGG 0.477610 4:+58985550 MsG0480021517.01.T01:CDS
ATTATGTCTTGCTCATGCTC+AGG 0.490388 4:-58984516 None:intergenic
TGGGCAACTTATGGGAGATA+AGG 0.492443 4:+58982556 MsG0480021517.01.T01:CDS
GGTACCAAAATACAATGTAT+CGG 0.506345 4:+58984568 MsG0480021517.01.T01:CDS
AAGCAAAATAGAAGATCCTC+TGG 0.513091 4:+58984696 MsG0480021517.01.T01:CDS
GTTGGAGGAGTCGACAAGAA+AGG 0.518202 4:+58985565 MsG0480021517.01.T01:CDS
GTACCAAAATACAATGTATC+GGG 0.520464 4:+58984569 MsG0480021517.01.T01:CDS
TTGGAGGAGTCGACAAGAAA+GGG 0.526623 4:+58985566 MsG0480021517.01.T01:CDS
CTAAAATGTCAGCTGAAACA+TGG 0.537484 4:+58985695 MsG0480021517.01.T01:CDS
TATTGCAGAGGATATATAAG+TGG 0.540656 4:+58984490 MsG0480021517.01.T01:intron
TATGGTTTGGAGACAAAGGT+AGG 0.552465 4:+58985595 MsG0480021517.01.T01:CDS
CACGTCTGTATCAGGGACTT+GGG 0.564292 4:+58982537 MsG0480021517.01.T01:CDS
CAGCTGAAACATGGAACTCA+AGG 0.566534 4:+58985704 MsG0480021517.01.T01:CDS
TTGGGAAGATAGTAATTGGA+AGG 0.576953 4:+58984656 MsG0480021517.01.T01:CDS
TCAGATGATCTGGATGAAGA+AGG 0.580244 4:-58985628 None:intergenic
TATCATCATAAGGCAACTAG+TGG 0.591044 4:+58984546 MsG0480021517.01.T01:CDS
GAGGAATAGACAAAGGCGAA+TGG 0.591694 4:+58984598 MsG0480021517.01.T01:CDS
GATTATGCACGTCTGTATCA+GGG 0.592853 4:+58982530 MsG0480021517.01.T01:CDS
CATTTATGGTTTGGAGACAA+AGG 0.600263 4:+58985591 MsG0480021517.01.T01:CDS
TAATCTTTGTAATGTCACGT+GGG 0.618544 4:-58982512 None:intergenic
AATCTTTGTAATGTCACGTG+GGG 0.628420 4:-58982511 None:intergenic
ACAATGTATCGGGTACAAAG+AGG 0.640532 4:+58984579 MsG0480021517.01.T01:CDS
AGATAGTAATTGGAAGGACA+AGG 0.647482 4:+58984662 MsG0480021517.01.T01:CDS
TCTTCATCCAGATCATCTGA+CGG 0.649115 4:+58985631 MsG0480021517.01.T01:CDS
AGATGAAGTTGTCTGACTGA+TGG 0.649405 4:-58985531 None:intergenic
AGTGTGCAACGGACCACCAG+AGG 0.655012 4:-58984712 None:intergenic
ATTGACTCACAAGTGTGCAA+CGG 0.668289 4:-58984723 None:intergenic
ATCATCATAAGGCAACTAGT+GGG 0.668292 4:+58984547 MsG0480021517.01.T01:CDS
GTACAAAGAGGAATAGACAA+AGG 0.671001 4:+58984591 MsG0480021517.01.T01:CDS
CAAAATAGAAGATCCTCTGG+TGG 0.672878 4:+58984699 MsG0480021517.01.T01:CDS
ATAATCTTTGTAATGTCACG+TGG 0.679434 4:-58982513 None:intergenic
ATCTTTGTAATGTCACGTGG+GGG 0.695485 4:-58982510 None:intergenic
GAAACATGGAACTCAAGGAG+CGG 0.696756 4:+58985709 MsG0480021517.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGATTTATTCTATTTTAT+TGG - Chr4:58984360-58984379 None:intergenic 10.0%
!! AAATATGCAAGTTATAATTT+AGG + Chr4:58984031-58984050 MsG0480021517.01.T01:intron 15.0%
!! AACAAATTATATTTGTAGTA+AGG + Chr4:58984781-58984800 MsG0480021517.01.T01:intron 15.0%
!! TAAAACATAATAGTTAATAG+TGG - Chr4:58983423-58983442 None:intergenic 15.0%
!!! ATAAAACAATGAAAATTTTC+TGG + Chr4:58985011-58985030 MsG0480021517.01.T01:intron 15.0%
!!! ATTATGTTTTATACAATCTT+TGG + Chr4:58983432-58983451 MsG0480021517.01.T01:intron 15.0%
!! AAAAATTATAGCATATCACA+TGG - Chr4:58983307-58983326 None:intergenic 20.0%
!! AATGAAAACTTATCATCATA+AGG + Chr4:58984536-58984555 MsG0480021517.01.T01:CDS 20.0%
!! ACAAGCAATGTAAATAATAT+AGG - Chr4:58984471-58984490 None:intergenic 20.0%
!! ATAATCTTATGTTTCTTCTT+TGG + Chr4:58985289-58985308 MsG0480021517.01.T01:intron 20.0%
!! ATGAAAAATTGGAACAAAAT+TGG + Chr4:58984637-58984656 MsG0480021517.01.T01:CDS 20.0%
!! ATGGATAACATTAAAAACTA+TGG - Chr4:58985231-58985250 None:intergenic 20.0%
!! GTAAAAAGAGATTAAGAAAA+GGG - Chr4:58982907-58982926 None:intergenic 20.0%
!! GTAAATAATATAGGATAAAC+CGG - Chr4:58984462-58984481 None:intergenic 20.0%
!! TATTGAATTGCATGAAAAAT+TGG + Chr4:58984626-58984645 MsG0480021517.01.T01:CDS 20.0%
!! TGAAAAATTGGAACAAAATT+GGG + Chr4:58984638-58984657 MsG0480021517.01.T01:CDS 20.0%
!! TGTAAAAAGAGATTAAGAAA+AGG - Chr4:58982908-58982927 None:intergenic 20.0%
!! TTAAAATTGTATTTGCTTCT+TGG + Chr4:58983709-58983728 MsG0480021517.01.T01:intron 20.0%
!!! AATTTTTCATGCAATTCAAT+AGG - Chr4:58984627-58984646 None:intergenic 20.0%
!!! AGATTTTACCAATTCTAAAA+TGG - Chr4:58983524-58983543 None:intergenic 20.0%
!!! ATTTTTCATGCAATTCAATA+GGG - Chr4:58984626-58984645 None:intergenic 20.0%
!!! GAATTAGTTATATCCTTTTT+TGG + Chr4:58985033-58985052 MsG0480021517.01.T01:intron 20.0%
!!! TAATTTGTTTAAGATGCTAT+TGG + Chr4:58985354-58985373 MsG0480021517.01.T01:intron 20.0%
!!! TATAACTTGCATATTTACTA+TGG - Chr4:58984027-58984046 None:intergenic 20.0%
!!! TGTTTTATGTATTTGACAAA+AGG - Chr4:58984999-58985018 None:intergenic 20.0%
! AAAATTGGGAAGATAGTAAT+TGG + Chr4:58984652-58984671 MsG0480021517.01.T01:CDS 25.0%
! AGAGCTGTATATATGTTAAT+TGG - Chr4:58982828-58982847 None:intergenic 25.0%
! AGCATTAGAATTCTATGATA+AGG - Chr4:58983024-58983043 None:intergenic 25.0%
! CATATGAATATAAGCATATG+AGG + Chr4:58985662-58985681 MsG0480021517.01.T01:CDS 25.0%
! CCAGCAATTTCAAATAAAAA+TGG - Chr4:58982687-58982706 None:intergenic 25.0%
! CTCATATGCTTATATTCATA+TGG - Chr4:58985664-58985683 None:intergenic 25.0%
! GCATTAGAATTCTATGATAA+GGG - Chr4:58983023-58983042 None:intergenic 25.0%
! TCCTACATTGATGAAAAAAT+GGG - Chr4:58983617-58983636 None:intergenic 25.0%
! TCTTGATTTCACAATTTCTT+TGG + Chr4:58983589-58983608 MsG0480021517.01.T01:intron 25.0%
! TCTTGGAATTATATTTCCAT+TGG - Chr4:58984060-58984079 None:intergenic 25.0%
! TTAGTAGTTACGATTCTATT+GGG - Chr4:58983062-58983081 None:intergenic 25.0%
!! CTTTATTTGTTTGTTTGTGT+TGG + Chr4:58983732-58983751 MsG0480021517.01.T01:intron 25.0%
!! TACTTTTCACTTATTAAGGA+TGG - Chr4:58982962-58982981 None:intergenic 25.0%
!! TAGAAGAGCTTAACATATTT+TGG - Chr4:58983657-58983676 None:intergenic 25.0%
!! TCTCTTTTTACATGAAATCA+AGG + Chr4:58982916-58982935 MsG0480021517.01.T01:intron 25.0%
!!! CCATTTTTATTTGAAATTGC+TGG + Chr4:58982684-58982703 MsG0480021517.01.T01:intron 25.0%
!!! GAAGTACTTTTCACTTATTA+AGG - Chr4:58982966-58982985 None:intergenic 25.0%
AAAAGAGATTAAGAAAAGGG+TGG - Chr4:58982904-58982923 None:intergenic 30.0%
AAGTTGTGTTTCAATCTTCT+TGG - Chr4:58984077-58984096 None:intergenic 30.0%
AGAGTTCGTCATAATTCTTA+CGG - Chr4:58985433-58985452 None:intergenic 30.0%
ATAATCTTTGTAATGTCACG+TGG - Chr4:58982516-58982535 None:intergenic 30.0%
ATATTTACTATGGCTACTGT+AGG - Chr4:58984017-58984036 None:intergenic 30.0%
ATTCTATGATAAGGGAATCA+TGG - Chr4:58983015-58983034 None:intergenic 30.0%
CAACAGGATTCAATAAGATA+TGG - Chr4:58983123-58983142 None:intergenic 30.0%
CAATAGAAACAAGAGAATAG+TGG + Chr4:58984376-58984395 MsG0480021517.01.T01:intron 30.0%
CATGCTTATTATTGTTCATC+AGG + Chr4:58985248-58985267 MsG0480021517.01.T01:intron 30.0%
CTCCTACATTGATGAAAAAA+TGG - Chr4:58983618-58983637 None:intergenic 30.0%
CTGATGAACAATAATAAGCA+TGG - Chr4:58985250-58985269 None:intergenic 30.0%
GAGAAATTGTCATGTTCTTT+AGG - Chr4:58984160-58984179 None:intergenic 30.0%
GGTACCAAAATACAATGTAT+CGG + Chr4:58984568-58984587 MsG0480021517.01.T01:CDS 30.0%
GTACCAAAATACAATGTATC+GGG + Chr4:58984569-58984588 MsG0480021517.01.T01:CDS 30.0%
GTTAGTAGTTACGATTCTAT+TGG - Chr4:58983063-58983082 None:intergenic 30.0%
GTTATAATTTAGGCTACCAA+TGG + Chr4:58984041-58984060 MsG0480021517.01.T01:intron 30.0%
TAATCTTTGTAATGTCACGT+GGG - Chr4:58982515-58982534 None:intergenic 30.0%
TATACGTTGAAGAACTCTTT+CGG + Chr4:58983870-58983889 MsG0480021517.01.T01:intron 30.0%
TATTGCAGAGGATATATAAG+TGG + Chr4:58984490-58984509 MsG0480021517.01.T01:intron 30.0%
TCACTGCCAAAAAAAATCAT+AGG - Chr4:58983966-58983985 None:intergenic 30.0%
TCATAATCAGATTACCATCA+AGG - Chr4:58983799-58983818 None:intergenic 30.0%
TGCCAAAAAAAATCATAGGA+TGG - Chr4:58983962-58983981 None:intergenic 30.0%
TTACTTCAAAGATCAGTACA+AGG - Chr4:58983495-58983514 None:intergenic 30.0%
! ATGAAAGACAGGTTTTTGAA+AGG + Chr4:58985470-58985489 MsG0480021517.01.T01:intron 30.0%
! ATGACGAACTCTAATTTTGT+AGG + Chr4:58985441-58985460 MsG0480021517.01.T01:intron 30.0%
! ATGTTGTATACATGCTTTTC+AGG + Chr4:58983373-58983392 MsG0480021517.01.T01:intron 30.0%
! GAAAAGCATGTATACAACAT+GGG - Chr4:58983373-58983392 None:intergenic 30.0%
! TCATGTGCTACAATTGTTTT+TGG + Chr4:58985163-58985182 MsG0480021517.01.T01:intron 30.0%
! TCTAAGACACACAAAATGAA+AGG + Chr4:58984896-58984915 MsG0480021517.01.T01:intron 30.0%
! TGAAAAGCATGTATACAACA+TGG - Chr4:58983374-58983393 None:intergenic 30.0%
! TGTTGTATACATGCTTTTCA+GGG + Chr4:58983374-58983393 MsG0480021517.01.T01:intron 30.0%
! TTGTTTAAGATGCTATTGGT+TGG + Chr4:58985358-58985377 MsG0480021517.01.T01:intron 30.0%
! TTTCAATGTTTTGTCCTTGA+TGG + Chr4:58983782-58983801 MsG0480021517.01.T01:intron 30.0%
!!! AGTGATGTTGTTTTTCTGAT+TGG + Chr4:58982849-58982868 MsG0480021517.01.T01:intron 30.0%
!!! ATCTTTTTTTCAACTGTCGT+CGG + Chr4:58984198-58984217 MsG0480021517.01.T01:intron 30.0%
!!! CACCATCCTATGATTTTTTT+TGG + Chr4:58983957-58983976 MsG0480021517.01.T01:intron 30.0%
!!! GTTATATCCTTTTTTGGACT+TGG + Chr4:58985039-58985058 MsG0480021517.01.T01:intron 30.0%
!!! TCCCATTTTTTCATCAATGT+AGG + Chr4:58983613-58983632 MsG0480021517.01.T01:intron 30.0%
AAGCAAAATAGAAGATCCTC+TGG + Chr4:58984696-58984715 MsG0480021517.01.T01:CDS 35.0%
AATCTTTGTAATGTCACGTG+GGG - Chr4:58982514-58982533 None:intergenic 35.0%
ACTTGTGTATAACGAGTTTC+AGG + Chr4:58982599-58982618 MsG0480021517.01.T01:CDS 35.0%
AGATAGTAATTGGAAGGACA+AGG + Chr4:58984662-58984681 MsG0480021517.01.T01:CDS 35.0%
AGATTACTAGATGATCGTGA+TGG + Chr4:58983551-58983570 MsG0480021517.01.T01:intron 35.0%
ATCATCATAAGGCAACTAGT+GGG + Chr4:58984547-58984566 MsG0480021517.01.T01:CDS 35.0%
ATGTATACAACATGGGACAT+GGG - Chr4:58983366-58983385 None:intergenic 35.0%
CAAGGACAAAACATTGAAAG+TGG - Chr4:58983781-58983800 None:intergenic 35.0%
CTAAAATGTCAGCTGAAACA+TGG + Chr4:58985695-58985714 MsG0480021517.01.T01:CDS 35.0%
CTAACTATAACTTCTCTCAG+TGG + Chr4:58982874-58982893 MsG0480021517.01.T01:intron 35.0%
GTACAAAGAGGAATAGACAA+AGG + Chr4:58984591-58984610 MsG0480021517.01.T01:CDS 35.0%
GTAGGATGAATATGAAAGAC+AGG + Chr4:58985459-58985478 MsG0480021517.01.T01:intron 35.0%
TACTGCAACCATATGAGATT+TGG - Chr4:58984945-58984964 None:intergenic 35.0%
TATCATCATAAGGCAACTAG+TGG + Chr4:58984546-58984565 MsG0480021517.01.T01:CDS 35.0%
TGCCTTCCAAAAATTCGAAA+AGG - Chr4:58984120-58984139 None:intergenic 35.0%
TTACATTGCTTGTATTGCAG+AGG + Chr4:58984478-58984497 MsG0480021517.01.T01:intron 35.0%
TTCATCTTCTTGGACATAGT+TGG + Chr4:58985547-58985566 MsG0480021517.01.T01:CDS 35.0%
TTGAATCCTGTTGTGTTTGA+AGG + Chr4:58983130-58983149 MsG0480021517.01.T01:intron 35.0%
TTGGGAAGATAGTAATTGGA+AGG + Chr4:58984656-58984675 MsG0480021517.01.T01:CDS 35.0%
! CATTTATGGTTTGGAGACAA+AGG + Chr4:58985591-58985610 MsG0480021517.01.T01:CDS 35.0%
! GTACCCGATACATTGTATTT+TGG - Chr4:58984575-58984594 None:intergenic 35.0%
! TAGCAGCCTTTTCGAATTTT+TGG + Chr4:58984111-58984130 MsG0480021517.01.T01:intron 35.0%
! TTGGTGTTTGCTTTCTGATT+GGG + Chr4:58983451-58983470 MsG0480021517.01.T01:intron 35.0%
!! AGCCTTTTCGAATTTTTGGA+AGG + Chr4:58984115-58984134 MsG0480021517.01.T01:intron 35.0%
!! TTTGGTGTTTGCTTTCTGAT+TGG + Chr4:58983450-58983469 MsG0480021517.01.T01:intron 35.0%
!!! AACGCGTACCATTTTAGAAT+TGG + Chr4:58983513-58983532 MsG0480021517.01.T01:intron 35.0%
!!! CTTTTAGAATGCTCAGTTGT+TGG - Chr4:58984750-58984769 None:intergenic 35.0%
AAAAATGATGGTAGGCAGTG+CGG - Chr4:58982726-58982745 None:intergenic 40.0%
AAAGCTCCTAGTTTGATGTC+CGG + Chr4:58984440-58984459 MsG0480021517.01.T01:intron 40.0%
AAGGAACTCACAGCAAACAT+AGG + Chr4:58985489-58985508 MsG0480021517.01.T01:intron 40.0%
ACAATGTATCGGGTACAAAG+AGG + Chr4:58984579-58984598 MsG0480021517.01.T01:CDS 40.0%
AGATGAAGTTGTCTGACTGA+TGG - Chr4:58985534-58985553 None:intergenic 40.0%
AGATTATGCACGTCTGTATC+AGG + Chr4:58982529-58982548 MsG0480021517.01.T01:CDS 40.0%
AGGAACTCACAGCAAACATA+GGG + Chr4:58985490-58985509 MsG0480021517.01.T01:intron 40.0%
AGGGAGCAAAAAATGATGGT+AGG - Chr4:58982734-58982753 None:intergenic 40.0%
ATCTTCTTGGACATAGTTGG+AGG + Chr4:58985550-58985569 MsG0480021517.01.T01:CDS 40.0%
ATCTTTGTAATGTCACGTGG+GGG - Chr4:58982513-58982532 None:intergenic 40.0%
ATTATGTCTTGCTCATGCTC+AGG - Chr4:58984519-58984538 None:intergenic 40.0%
ATTCCATACCCTCCAAAGTT+GGG - Chr4:58982757-58982776 None:intergenic 40.0%
CAAAATAGAAGATCCTCTGG+TGG + Chr4:58984699-58984718 MsG0480021517.01.T01:CDS 40.0%
CACCTGCCTTTAATAATGTC+CGG + Chr4:58984237-58984256 MsG0480021517.01.T01:intron 40.0%
CAGCATACCAAGTCCAAAAA+AGG - Chr4:58985049-58985068 None:intergenic 40.0%
CATGTATACAACATGGGACA+TGG - Chr4:58983367-58983386 None:intergenic 40.0%
CGAGGACGTTAAACATAAGT+TGG - Chr4:58982643-58982662 None:intergenic 40.0%
GATAAACCGGACATCAAACT+AGG - Chr4:58984449-58984468 None:intergenic 40.0%
GATTATGCACGTCTGTATCA+GGG + Chr4:58982530-58982549 MsG0480021517.01.T01:CDS 40.0%
GGTATCCTCCAAATCTCATA+TGG + Chr4:58984934-58984953 MsG0480021517.01.T01:intron 40.0%
GTCAGACAACTTCATCTTCT+TGG + Chr4:58985537-58985556 MsG0480021517.01.T01:CDS 40.0%
TACTTGTGTCTCTGTATGCT+AGG + Chr4:58983684-58983703 MsG0480021517.01.T01:intron 40.0%
TCAGATGATCTGGATGAAGA+AGG - Chr4:58985631-58985650 None:intergenic 40.0%
TCTTCATCCAGATCATCTGA+CGG + Chr4:58985631-58985650 MsG0480021517.01.T01:CDS 40.0%
TGCAACCATATGAGATTTGG+AGG - Chr4:58984942-58984961 None:intergenic 40.0%
TGTGAACCTTCAAACACAAC+AGG - Chr4:58983139-58983158 None:intergenic 40.0%
! ACATAAGTTGGAGGAAGAGA+AGG - Chr4:58982631-58982650 None:intergenic 40.0%
! ATTGACTCACAAGTGTGCAA+CGG - Chr4:58984726-58984745 None:intergenic 40.0%
! GGACGTTAAACATAAGTTGG+AGG - Chr4:58982640-58982659 None:intergenic 40.0%
! GTTTGTTTGTGTTGGTCTAC+TGG + Chr4:58983740-58983759 MsG0480021517.01.T01:intron 40.0%
! TATGGTTTGGAGACAAAGGT+AGG + Chr4:58985595-58985614 MsG0480021517.01.T01:CDS 40.0%
! TCGTCATGTGTATTGATGCA+TGG + Chr4:58985119-58985138 MsG0480021517.01.T01:intron 40.0%
!!! CTTTTTTGGACTTGGTATGC+TGG + Chr4:58985047-58985066 MsG0480021517.01.T01:intron 40.0%
AAGGCATGTGAACAATGTGG+GGG + Chr4:58984134-58984153 MsG0480021517.01.T01:intron 45.0%
AAGTTGGAGGAAGAGAAGGA+CGG - Chr4:58982627-58982646 None:intergenic 45.0%
CACCGGACATTATTAAAGGC+AGG - Chr4:58984242-58984261 None:intergenic 45.0%
CAGCTGAAACATGGAACTCA+AGG + Chr4:58985704-58985723 MsG0480021517.01.T01:CDS 45.0%
CATTCCATACCCTCCAAAGT+TGG - Chr4:58982758-58982777 None:intergenic 45.0%
CCACCACCGGACATTATTAA+AGG - Chr4:58984246-58984265 None:intergenic 45.0%
CCTTTAATAATGTCCGGTGG+TGG + Chr4:58984243-58984262 MsG0480021517.01.T01:intron 45.0%
CTGCCTTTAATAATGTCCGG+TGG + Chr4:58984240-58984259 MsG0480021517.01.T01:intron 45.0%
GAAACATGGAACTCAAGGAG+CGG + Chr4:58985709-58985728 MsG0480021517.01.T01:CDS 45.0%
GAAAGGCGATCCATCAAAGT+GGG + Chr4:58984913-58984932 MsG0480021517.01.T01:intron 45.0%
GAACTCACAGCAAACATAGG+GGG + Chr4:58985492-58985511 MsG0480021517.01.T01:intron 45.0%
GAAGGTTCACAGACACTAGT+CGG + Chr4:58983148-58983167 MsG0480021517.01.T01:intron 45.0%
GAGCGACAAATCTAACAACG+AGG - Chr4:58982661-58982680 None:intergenic 45.0%
GAGGAATAGACAAAGGCGAA+TGG + Chr4:58984598-58984617 MsG0480021517.01.T01:CDS 45.0%
GGAACTCACAGCAAACATAG+GGG + Chr4:58985491-58985510 MsG0480021517.01.T01:intron 45.0%
TGAAAGGCGATCCATCAAAG+TGG + Chr4:58984912-58984931 MsG0480021517.01.T01:intron 45.0%
TGGGAGGGAGCAAAAAATGA+TGG - Chr4:58982738-58982757 None:intergenic 45.0%
TGGGCAACTTATGGGAGATA+AGG + Chr4:58982556-58982575 MsG0480021517.01.T01:CDS 45.0%
TTGGAGGAGTCGACAAGAAA+GGG + Chr4:58985566-58985585 MsG0480021517.01.T01:CDS 45.0%
TTGGAGGATACCCACTTTGA+TGG - Chr4:58984926-58984945 None:intergenic 45.0%
! CAACTTTGGAGGGTATGGAA+TGG + Chr4:58982756-58982775 MsG0480021517.01.T01:intron 45.0%
! GAAAGGGCGCATTTATGGTT+TGG + Chr4:58985582-58985601 MsG0480021517.01.T01:CDS 45.0%
! GAAGGCATGTGAACAATGTG+GGG + Chr4:58984133-58984152 MsG0480021517.01.T01:intron 45.0%
! GACAAGAAAGGGCGCATTTA+TGG + Chr4:58985577-58985596 MsG0480021517.01.T01:CDS 45.0%
! GGAAGGCATGTGAACAATGT+GGG + Chr4:58984132-58984151 MsG0480021517.01.T01:intron 45.0%
! TGGAAGGCATGTGAACAATG+TGG + Chr4:58984131-58984150 MsG0480021517.01.T01:intron 45.0%
! TTTTTGCTCCCTCCCAACTT+TGG + Chr4:58982742-58982761 MsG0480021517.01.T01:intron 45.0%
!! TTTTGGACTTGGTATGCTGG+TGG + Chr4:58985050-58985069 MsG0480021517.01.T01:intron 45.0%
AAACCTGACTCCAACCTGCT+GGG + Chr4:58984866-58984885 MsG0480021517.01.T01:intron 50.0%
CAACAACAGTTGACCACCAC+CGG - Chr4:58984259-58984278 None:intergenic 50.0%
CACGTCTGTATCAGGGACTT+GGG + Chr4:58982537-58982556 MsG0480021517.01.T01:CDS 50.0%
CAGGGACTTGGGCAACTTAT+GGG + Chr4:58982548-58982567 MsG0480021517.01.T01:CDS 50.0%
CATACCCTCCAAAGTTGGGA+GGG - Chr4:58982753-58982772 None:intergenic 50.0%
GGACATGGGTGCAAAAGACA+CGG - Chr4:58983352-58983371 None:intergenic 50.0%
GGTATGCTGGTGGAAAGTCA+AGG + Chr4:58985060-58985079 MsG0480021517.01.T01:intron 50.0%
GTTGGAGGAGTCGACAAGAA+AGG + Chr4:58985565-58985584 MsG0480021517.01.T01:CDS 50.0%
GTTGTTGGGTCAACGACAGT+TGG + Chr4:58984272-58984291 MsG0480021517.01.T01:intron 50.0%
TCAGGGACTTGGGCAACTTA+TGG + Chr4:58982547-58982566 MsG0480021517.01.T01:CDS 50.0%
TCTTAGATACACCTCCCAGC+AGG - Chr4:58984883-58984902 None:intergenic 50.0%
TGAGATGCCGTCAGATGATC+TGG - Chr4:58985641-58985660 None:intergenic 50.0%
! ATAGTGGAGTGCAGTGTCAG+TGG - Chr4:58983407-58983426 None:intergenic 50.0%
! GGTGGTGGTCAACTGTTGTT+GGG + Chr4:58984258-58984277 MsG0480021517.01.T01:intron 50.0%
AATGATGGTAGGCAGTGCGG+TGG - Chr4:58982723-58982742 None:intergenic 55.0%
AGATACACCTCCCAGCAGGT+TGG - Chr4:58984879-58984898 None:intergenic 55.0%
CAAACCTGACTCCAACCTGC+TGG + Chr4:58984865-58984884 MsG0480021517.01.T01:intron 55.0%
CCATACCCTCCAAAGTTGGG+AGG - Chr4:58982754-58982773 None:intergenic 55.0%
GCACGTCTGTATCAGGGACT+TGG + Chr4:58982536-58982555 MsG0480021517.01.T01:CDS 55.0%
TGCTCCCTCCCAACTTTGGA+GGG + Chr4:58982746-58982765 MsG0480021517.01.T01:intron 55.0%
TTGCTCCCTCCCAACTTTGG+AGG + Chr4:58982745-58982764 MsG0480021517.01.T01:intron 55.0%
! CCTCCCAACTTTGGAGGGTA+TGG + Chr4:58982751-58982770 MsG0480021517.01.T01:intron 55.0%
! CGGTGGTGGTCAACTGTTGT+TGG + Chr4:58984257-58984276 MsG0480021517.01.T01:intron 55.0%
AGTGTGCAACGGACCACCAG+AGG - Chr4:58984715-58984734 None:intergenic 60.0%
CCTGACTCCAACCTGCTGGG+AGG + Chr4:58984869-58984888 MsG0480021517.01.T01:intron 65.0%
! CCTCCCAGCAGGTTGGAGTC+AGG - Chr4:58984872-58984891 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 58982492 58985735 58982492 ID=MsG0480021517.01;Name=MsG0480021517.01
Chr4 mRNA 58982492 58985735 58982492 ID=MsG0480021517.01.T01;Parent=MsG0480021517.01;Name=MsG0480021517.01.T01;_AED=0.23;_eAED=0.23;_QI=0|0|0|1|0.5|0.33|3|0|209
Chr4 exon 58982492 58982620 58982492 ID=MsG0480021517.01.T01:exon:17464;Parent=MsG0480021517.01.T01
Chr4 exon 58984498 58984773 58984498 ID=MsG0480021517.01.T01:exon:17465;Parent=MsG0480021517.01.T01
Chr4 exon 58985511 58985735 58985511 ID=MsG0480021517.01.T01:exon:17466;Parent=MsG0480021517.01.T01
Chr4 CDS 58982492 58982620 58982492 ID=MsG0480021517.01.T01:cds;Parent=MsG0480021517.01.T01
Chr4 CDS 58984498 58984773 58984498 ID=MsG0480021517.01.T01:cds;Parent=MsG0480021517.01.T01
Chr4 CDS 58985511 58985735 58985511 ID=MsG0480021517.01.T01:cds;Parent=MsG0480021517.01.T01
Gene Sequence

>MsG0480021517.01.T01

TTTGAGCTTGTTGTTCAGCCCCCACGTGACATTACAAAGATTATGCACGTCTGTATCAGGGACTTGGGCAACTTATGGGAGATAAGGAAAAATGACAAGATCTTGCAACTTGTGTATAACGAGTTTCAGAGGATATATAAGTGGCTTCCTGAGCATGAGCAAGACATAATGAAAACTTATCATCATAAGGCAACTAGTGGGTACCAAAATACAATGTATCGGGTACAAAGAGGAATAGACAAAGGCGAATGGATCCCTATTGAATTGCATGAAAAATTGGAACAAAATTGGGAAGATAGTAATTGGAAGGACAAGGCAGAAGTTAATAAGCAAAATAGAAGATCCTCTGGTGGTCCGTTGCACACTTGTGAGTCAATTCCAACAACTGAGCATTCTAAAAGATTAGGGGATGATGTGTCTAATGACCATCAGTCAGACAACTTCATCTTCTTGGACATAGTTGGAGGAGTCGACAAGAAAGGGCGCATTTATGGTTTGGAGACAAAGGTAGGAAAATACAAGCCTTCTTCATCCAGATCATCTGACGGCATCTCACCATATGAATATAAGCATATGAGGATTGCAATATCTAAAATGTCAGCTGAAACATGGAACTCAAGGAGCGGTTGA
Protein sequence

>MsG0480021517.01.T01

FELVVQPPRDITKIMHVCIRDLGNLWEIRKNDKILQLVYNEFQRIYKWLPEHEQDIMKTYHHKATSGYQNTMYRVQRGIDKGEWIPIELHEKLEQNWEDSNWKDKAEVNKQNRRSSGGPLHTCESIPTTEHSKRLGDDVSNDHQSDNFIFLDIVGGVDKKGRIYGLETKVGKYKPSSSRSSDGISPYEYKHMRIAISKMSAETWNSRSG*