AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180006134.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180006134.01.T01 MTR_1g041885 65.476 84 28 1 1 84 414 496 1.43e-36 129
MsG0180006134.01.T01 MTR_1g041875 65.854 82 27 1 1 82 461 541 2.78e-35 125
MsG0180006134.01.T01 MTR_1g042310 63.095 84 30 1 1 84 421 503 7.50e-35 124
MsG0180006134.01.T01 MTR_1g042310 63.095 84 30 1 1 84 428 510 9.05e-35 124
MsG0180006134.01.T01 MTR_1g042310 63.095 84 30 1 1 84 465 547 1.01e-34 124
MsG0180006134.01.T01 MTR_1g041835 57.647 85 35 1 1 85 465 548 8.61e-33 119
MsG0180006134.01.T01 MTR_1g041815 56.471 85 36 1 1 85 465 548 3.66e-32 117
MsG0180006134.01.T01 MTR_1g075440 55.952 84 36 1 1 84 467 549 1.74e-29 109
MsG0180006134.01.T01 MTR_7g098200 52.941 85 39 1 1 85 354 437 3.59e-29 108
MsG0180006134.01.T01 MTR_7g098200 52.941 85 39 1 1 85 458 541 6.07e-29 108
MsG0180006134.01.T01 MTR_7g098200 52.941 85 39 1 1 85 468 551 6.82e-29 107
MsG0180006134.01.T01 MTR_7g098180 55.128 78 34 1 8 85 478 554 1.26e-27 104
MsG0180006134.01.T01 MTR_7g098210 49.412 85 42 1 1 85 343 426 2.87e-26 100
MsG0180006134.01.T01 MTR_7g098230 50.000 84 41 1 1 84 465 547 4.08e-26 100
MsG0180006134.01.T01 MTR_7g098160 53.846 78 35 1 8 85 479 555 8.35e-26 99.4
MsG0180006134.01.T01 MTR_7g098090 50.000 84 41 1 2 85 478 560 4.16e-25 97.4
MsG0180006134.01.T01 MTR_7g098220 50.000 78 38 1 8 85 432 508 2.28e-24 95.1
MsG0180006134.01.T01 MTR_7g098220 50.000 78 38 1 8 85 476 552 2.51e-24 95.1
MsG0180006134.01.T01 MTR_7g098040 48.718 78 39 1 8 85 480 556 1.68e-23 92.8
MsG0180006134.01.T01 MTR_3g072300 40.476 84 49 1 1 84 462 544 1.02e-18 79.0
MsG0180006134.01.T01 MTR_7g067010 39.286 84 50 1 1 84 462 544 1.47e-18 78.6
MsG0180006134.01.T01 MTR_4g033390 47.887 71 36 1 15 85 30 99 3.73e-18 73.2
MsG0180006134.01.T01 MTR_4g015080 38.824 85 51 1 1 85 472 555 6.27e-18 77.0
MsG0180006134.01.T01 MTR_4g033325 42.857 84 47 1 2 85 327 409 2.71e-17 75.1
MsG0180006134.01.T01 MTR_4g033325 42.857 84 47 1 2 85 353 435 2.77e-17 75.1
MsG0180006134.01.T01 MTR_4g033325 42.857 84 47 1 2 85 451 533 3.09e-17 74.7
MsG0180006134.01.T01 MTR_3g462710 37.647 85 52 1 1 85 355 438 5.14e-17 74.3
MsG0180006134.01.T01 MTR_3g462710 37.647 85 52 1 1 85 464 547 5.50e-17 74.3
MsG0180006134.01.T01 MTR_3g005010 43.373 83 46 1 2 84 445 526 8.41e-17 73.6
MsG0180006134.01.T01 MTR_0294s0020 41.667 84 48 1 2 85 451 533 9.95e-17 73.6
MsG0180006134.01.T01 MTR_4g033310 41.667 84 48 1 2 85 389 471 2.79e-16 72.0
MsG0180006134.01.T01 MTR_4g033315 41.667 84 48 1 2 85 453 535 2.91e-16 72.0
MsG0180006134.01.T01 MTR_4g033310 41.667 84 48 1 2 85 453 535 2.91e-16 72.0
MsG0180006134.01.T01 MTR_4g033385 40.476 84 49 1 2 85 354 436 4.88e-16 71.6
MsG0180006134.01.T01 MTR_4g033330 42.683 82 46 1 2 83 453 533 5.00e-16 71.6
MsG0180006134.01.T01 MTR_3g069440 42.254 71 39 1 14 84 483 551 6.18e-16 71.2
MsG0180006134.01.T01 MTR_4g024930 37.349 83 51 1 1 83 456 537 1.12e-14 67.8
MsG0180006134.01.T01 MTR_3g069500 40.845 71 40 1 14 84 475 543 4.00e-14 66.2
MsG0180006134.01.T01 MTR_3g014790 39.437 71 41 1 14 84 462 530 1.17e-13 64.7
MsG0180006134.01.T01 MTR_8g038880 40.845 71 40 2 14 84 465 533 5.09e-13 62.8
MsG0180006134.01.T01 MTR_4g114340 36.486 74 46 1 11 84 470 542 8.79e-13 62.4
MsG0180006134.01.T01 MTR_7g010820 27.273 77 56 0 1 77 471 547 1.33e-12 61.6
MsG0180006134.01.T01 MTR_1g026740 38.571 70 42 1 14 83 470 538 1.92e-12 61.2
MsG0180006134.01.T01 MTR_4g098800 30.120 83 57 1 1 83 541 622 2.01e-12 61.2
MsG0180006134.01.T01 MTR_4g098800 30.120 83 57 1 1 83 476 557 2.04e-12 61.2
MsG0180006134.01.T01 MTR_5g014150 30.120 83 57 1 1 83 489 570 2.73e-12 60.8
MsG0180006134.01.T01 MTR_1g085090 37.838 74 44 2 13 84 456 529 4.45e-12 60.1
MsG0180006134.01.T01 MTR_3g069420 35.211 71 44 1 14 84 484 552 5.73e-12 60.1
MsG0180006134.01.T01 MTR_4g019580 35.366 82 47 2 2 83 476 551 7.76e-12 59.7
MsG0180006134.01.T01 MTR_1g116930 27.711 83 59 1 1 83 458 539 9.33e-12 59.3
MsG0180006134.01.T01 MTR_7g111260 37.838 74 44 2 13 84 456 529 1.02e-11 59.3
MsG0180006134.01.T01 MTR_2g101560 38.889 72 43 1 14 85 483 553 1.47e-11 58.9
MsG0180006134.01.T01 MTR_7g013270 25.000 84 62 1 1 84 527 609 2.97e-11 57.8
MsG0180006134.01.T01 MTR_7g006540 31.395 86 58 1 1 85 511 596 4.26e-11 57.4
MsG0180006134.01.T01 MTR_4g123537 38.667 75 44 2 13 85 480 554 5.58e-11 57.0
MsG0180006134.01.T01 MTR_4g107530 29.762 84 58 1 2 85 483 565 7.77e-11 56.6
MsG0180006134.01.T01 MTR_7g065080 31.707 82 50 2 2 83 378 453 8.96e-11 56.6
MsG0180006134.01.T01 MTR_7g065080 31.707 82 50 2 2 83 465 540 9.70e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180006134.01.T01 AT5G46050 49.383 81 39 1 1 81 354 432 2.79e-24 94.7
MsG0180006134.01.T01 AT5G46050 49.383 81 39 1 1 81 466 544 4.41e-24 94.4
MsG0180006134.01.T01 AT5G46040 47.561 82 41 1 1 82 466 545 1.74e-22 89.7
MsG0180006134.01.T01 AT2G02040 38.824 85 51 1 1 85 482 565 2.79e-18 77.8
MsG0180006134.01.T01 AT2G40460 40.000 85 50 1 1 85 456 539 4.20e-18 77.4
MsG0180006134.01.T01 AT3G54140 39.286 84 50 1 1 84 464 546 1.20e-17 76.3
MsG0180006134.01.T01 AT3G54140 39.286 84 50 1 1 84 464 546 1.20e-17 76.3
MsG0180006134.01.T01 AT5G01180 40.476 84 49 1 1 84 464 546 7.57e-17 73.9
MsG0180006134.01.T01 AT5G01180 40.476 84 49 1 1 84 464 546 7.57e-17 73.9
MsG0180006134.01.T01 AT5G01180 40.476 84 49 1 1 84 464 546 7.57e-17 73.9
MsG0180006134.01.T01 AT1G62200 36.471 85 53 1 1 85 517 600 2.98e-16 72.0
MsG0180006134.01.T01 AT1G62200 36.471 85 53 1 1 85 488 571 3.00e-16 72.0
MsG0180006134.01.T01 AT1G62200 36.471 85 53 1 1 85 531 614 3.13e-16 72.0
MsG0180006134.01.T01 AT1G22540 40.964 83 48 1 2 84 454 535 3.63e-16 72.0
MsG0180006134.01.T01 AT3G54450 43.662 71 38 2 14 84 457 525 9.51e-16 70.9
MsG0180006134.01.T01 AT1G72140 37.349 83 51 1 2 84 454 535 2.13e-14 67.0
MsG0180006134.01.T01 AT1G72130 38.571 70 42 1 14 83 447 515 6.53e-14 65.5
MsG0180006134.01.T01 AT1G72130 38.571 70 42 1 14 83 329 397 6.75e-14 65.5
MsG0180006134.01.T01 AT1G72130 38.571 70 42 1 14 83 329 397 6.75e-14 65.5
MsG0180006134.01.T01 AT5G19640 28.916 83 59 0 1 83 484 566 1.03e-13 65.1
MsG0180006134.01.T01 AT1G22550 37.037 81 50 1 1 81 460 539 2.37e-13 63.9
MsG0180006134.01.T01 AT1G22570 43.750 64 32 1 14 77 440 499 2.53e-13 63.9
MsG0180006134.01.T01 AT1G22570 43.750 64 32 1 14 77 477 536 3.03e-13 63.5
MsG0180006134.01.T01 AT2G02020 34.118 85 51 2 1 84 446 526 3.49e-13 63.5
MsG0180006134.01.T01 AT2G02020 34.118 85 51 2 1 84 425 505 3.88e-13 63.2
MsG0180006134.01.T01 AT2G26690 37.838 74 45 1 11 84 436 508 1.82e-12 61.2
MsG0180006134.01.T01 AT2G26690 37.838 74 45 1 11 84 469 541 1.90e-12 61.2
MsG0180006134.01.T01 AT2G26690 37.838 74 45 1 11 84 424 496 2.10e-12 61.2
MsG0180006134.01.T01 AT1G32450 30.120 83 57 1 1 83 485 566 4.81e-12 60.1
MsG0180006134.01.T01 AT1G52190 33.735 83 54 1 2 84 471 552 7.24e-12 59.7

Find 12 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGATTGACTGTTGTTATAT+TGG 0.272345 1:+100566464 MsG0180006134.01.T01:CDS
TCCACAAAACATGAAAAGCT+TGG 0.495027 1:+100566521 MsG0180006134.01.T01:CDS
CCACAAAACATGAAAAGCTT+GGG 0.503310 1:+100566522 MsG0180006134.01.T01:CDS
GGACACATGGACAGCGAGGG+TGG 0.541886 1:+100566628 MsG0180006134.01.T01:CDS
TTAAATTCAGATGTTCAAAA+CGG 0.548418 1:-100566703 None:intergenic
TCTCTAAGCACTACAGCAGT+CGG 0.548448 1:+100566555 MsG0180006134.01.T01:CDS
ATTGACTGTTGTTATATTGG+AGG 0.556187 1:+100566467 MsG0180006134.01.T01:CDS
CAATTTCACATGTAACATCA+AGG 0.589037 1:+100566607 MsG0180006134.01.T01:CDS
CATGTAACATCAAGGACACA+TGG 0.590007 1:+100566615 MsG0180006134.01.T01:CDS
GACACATGGACAGCGAGGGT+GGG 0.614450 1:+100566629 MsG0180006134.01.T01:CDS
CAAGGACACATGGACAGCGA+GGG 0.647228 1:+100566625 MsG0180006134.01.T01:CDS
TCAAGGACACATGGACAGCG+AGG 0.669696 1:+100566624 MsG0180006134.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATTGACTGTTGTTATATTGG+AGG + Chr1:100566467-100566486 MsG0180006134.01.T01:CDS 30.0%
CAATTTCACATGTAACATCA+AGG + Chr1:100566607-100566626 MsG0180006134.01.T01:CDS 30.0%
! TTTTCATGTTTTGTGGAGTT+TGG - Chr1:100566518-100566537 None:intergenic 30.0%
CCACAAAACATGAAAAGCTT+GGG + Chr1:100566522-100566541 MsG0180006134.01.T01:CDS 35.0%
GGGATTGACTGTTGTTATAT+TGG + Chr1:100566464-100566483 MsG0180006134.01.T01:CDS 35.0%
TCCACAAAACATGAAAAGCT+TGG + Chr1:100566521-100566540 MsG0180006134.01.T01:CDS 35.0%
CATGTAACATCAAGGACACA+TGG + Chr1:100566615-100566634 MsG0180006134.01.T01:CDS 40.0%
! CCCAAGCTTTTCATGTTTTG+TGG - Chr1:100566525-100566544 None:intergenic 40.0%
TCTCTAAGCACTACAGCAGT+CGG + Chr1:100566555-100566574 MsG0180006134.01.T01:CDS 45.0%
CAAGGACACATGGACAGCGA+GGG + Chr1:100566625-100566644 MsG0180006134.01.T01:CDS 55.0%
TCAAGGACACATGGACAGCG+AGG + Chr1:100566624-100566643 MsG0180006134.01.T01:CDS 55.0%
GACACATGGACAGCGAGGGT+GGG + Chr1:100566629-100566648 MsG0180006134.01.T01:CDS 60.0%
GGACACATGGACAGCGAGGG+TGG + Chr1:100566628-100566647 MsG0180006134.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 100566462 100566719 100566462 ID=MsG0180006134.01;Name=MsG0180006134.01
Chr1 mRNA 100566462 100566719 100566462 ID=MsG0180006134.01.T01;Parent=MsG0180006134.01;Name=MsG0180006134.01.T01;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|0|1|0|85
Chr1 exon 100566462 100566719 100566462 ID=MsG0180006134.01.T01:exon:3028;Parent=MsG0180006134.01.T01
Chr1 CDS 100566462 100566719 100566462 ID=MsG0180006134.01.T01:cds;Parent=MsG0180006134.01.T01
Gene Sequence

>MsG0180006134.01.T01

ATGGGATTGACTGTTGTTATATTGGAGGCAGCAAAAATGAAATTTTTCTATGACCAAACTCCACAAAACATGAAAAGCTTGGGTTCAACTTATTCTCTAAGCACTACAGCAGTCGGAAGTTTCTTAAGTACATTTTTCATGTCAACAATTTCACATGTAACATCAAGGACACATGGACAGCGAGGGTGGGTTCAAGACAACATTAATGCGTCTCATATAGATTATTATTATGCTTTTTTAGCCGTTTTGAACATCTGA

Protein sequence

>MsG0180006134.01.T01

MGLTVVILEAAKMKFFYDQTPQNMKSLGSTYSLSTTAVGSFLSTFFMSTISHVTSRTHGQRGWVQDNINASHIDYYYAFLAVLNI*