Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021663.01.T01 | PNX69283.1 | 89.404 | 151 | 16 | 0 | 1 | 151 | 58 | 208 | 3.76E-98 | 292 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021663.01.T01 | Q94HW2 | 54 | 150 | 69 | 0 | 1 | 150 | 1273 | 1422 | 1.52E-48 | 170 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021663.01.T01 | A0A2K3KSN1 | 89.404 | 151 | 16 | 0 | 1 | 151 | 58 | 208 | 1.80e-98 | 292 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480021663.01 | MsG0580027332.01 | 0.812059 | 5.130061e-51 | 3.228349e-48 |
| MsG0480021663.01 | MsG0780039282.01 | 0.803562 | 3.285550e-49 | 1.656447e-46 |
| MsG0280009248.01 | MsG0480021663.01 | 0.817211 | 3.710120e-52 | 2.685121e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021663.01.T01 | MTR_3g093120 | 41.722 | 151 | 87 | 1 | 1 | 151 | 341 | 490 | 7.28e-39 | 138 |
| MsG0480021663.01.T01 | MTR_6g472230 | 37.162 | 148 | 93 | 0 | 1 | 148 | 21 | 168 | 3.46e-33 | 123 |
| MsG0480021663.01.T01 | MTR_7g026095 | 43.750 | 112 | 63 | 0 | 1 | 112 | 54 | 165 | 1.14e-27 | 105 |
| MsG0480021663.01.T01 | MTR_8g059225 | 45.000 | 100 | 54 | 1 | 51 | 150 | 74 | 172 | 4.56e-27 | 100 |
| MsG0480021663.01.T01 | MTR_2g075320 | 40.336 | 119 | 54 | 1 | 32 | 150 | 146 | 247 | 4.26e-26 | 100 |
| MsG0480021663.01.T01 | MTR_8g011410 | 37.086 | 151 | 73 | 2 | 2 | 151 | 815 | 944 | 1.67e-25 | 101 |
| MsG0480021663.01.T01 | MTR_7g056567 | 51.389 | 72 | 35 | 0 | 79 | 150 | 23 | 94 | 3.32e-19 | 79.3 |
| MsG0480021663.01.T01 | MTR_6g060522 | 46.154 | 78 | 42 | 0 | 73 | 150 | 2 | 79 | 4.13e-19 | 79.7 |
| MsG0480021663.01.T01 | MTR_3g452820 | 33.775 | 151 | 58 | 5 | 1 | 150 | 49 | 158 | 1.94e-18 | 77.8 |
| MsG0480021663.01.T01 | MTR_6g060910 | 40.964 | 83 | 49 | 0 | 14 | 96 | 42 | 124 | 1.15e-17 | 74.3 |
| MsG0480021663.01.T01 | MTR_1g051400 | 36.000 | 100 | 64 | 0 | 16 | 115 | 73 | 172 | 1.87e-17 | 75.1 |
| MsG0480021663.01.T01 | MTR_3g448730 | 38.095 | 84 | 51 | 1 | 67 | 150 | 102 | 184 | 1.97e-16 | 73.6 |
| MsG0480021663.01.T01 | MTR_6g033925 | 41.176 | 85 | 47 | 1 | 2 | 86 | 54 | 135 | 1.24e-14 | 68.6 |
| MsG0480021663.01.T01 | MTR_2g017850 | 37.391 | 115 | 51 | 4 | 11 | 122 | 205 | 301 | 2.01e-14 | 69.3 |
| MsG0480021663.01.T01 | MTR_8g061995 | 90.000 | 30 | 3 | 0 | 116 | 145 | 1 | 30 | 2.28e-14 | 64.3 |
| MsG0480021663.01.T01 | MTR_1g058200 | 40.260 | 77 | 46 | 0 | 14 | 90 | 62 | 138 | 3.50e-13 | 63.5 |
| MsG0480021663.01.T01 | MTR_0303s0010 | 32.673 | 101 | 58 | 1 | 8 | 98 | 64 | 164 | 4.42e-12 | 60.8 |
| MsG0480021663.01.T01 | MTR_0233s0060 | 38.889 | 72 | 44 | 0 | 37 | 108 | 7 | 78 | 7.69e-12 | 62.0 |
| MsG0480021663.01.T01 | MTR_3g022740 | 28.947 | 114 | 54 | 1 | 37 | 150 | 45 | 131 | 9.57e-12 | 62.0 |
| MsG0480021663.01.T01 | MTR_8g096430 | 34.615 | 78 | 51 | 0 | 64 | 141 | 80 | 157 | 4.33e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021663.01.T01 | AT4G23160 | 42.177 | 147 | 85 | 0 | 3 | 149 | 408 | 554 | 1.05e-38 | 139 |
| MsG0480021663.01.T01 | AT4G23160 | 42.177 | 147 | 85 | 0 | 3 | 149 | 408 | 554 | 1.05e-38 | 139 |
Find 32 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTAAGGAAACCCGTACTA+TGG | 0.399263 | 4:-62470754 | MsG0480021663.01.T01:CDS |
| TCCACTGACTTGGATATAAC+AGG | 0.416902 | 4:-62470989 | MsG0480021663.01.T01:CDS |
| TTGGTGAAACACTTGTCTCT+TGG | 0.426551 | 4:-62470898 | MsG0480021663.01.T01:CDS |
| AGGCTTCTCTGATGCAGATT+GGG | 0.433768 | 4:-62470969 | MsG0480021663.01.T01:CDS |
| CAGGCTTCTCTGATGCAGAT+TGG | 0.451079 | 4:-62470970 | MsG0480021663.01.T01:CDS |
| GATGTGCAAGCAATGATTGA+TGG | 0.453674 | 4:+62471018 | None:intergenic |
| TGTTGATGATAGAAAATCTA+TGG | 0.455472 | 4:-62470939 | MsG0480021663.01.T01:CDS |
| TTAGAACGAGCATGCAGAAC+TGG | 0.492582 | 4:+62470695 | None:intergenic |
| TAAGTTATCACACCATAGTA+CGG | 0.497812 | 4:+62470742 | None:intergenic |
| CATCAAACCATCCACTGACT+TGG | 0.498056 | 4:-62470999 | MsG0480021663.01.T01:CDS |
| TTGGTCCTCAAGAAAACAAA+AGG | 0.499625 | 4:-62470879 | MsG0480021663.01.T01:CDS |
| GTACGGGTTTCCTTAGCAAT+GGG | 0.500820 | 4:+62470759 | None:intergenic |
| AAGTTATCACACCATAGTAC+GGG | 0.529161 | 4:+62470743 | None:intergenic |
| TAACTTAAGTGCCAAGGCAC+TGG | 0.531950 | 4:-62470726 | MsG0480021663.01.T01:CDS |
| GCTGGTCAATGTGTGTTCCT+TGG | 0.534274 | 4:-62470917 | MsG0480021663.01.T01:CDS |
| AGCAATGATTGATGGTGCCT+TGG | 0.537336 | 4:+62471026 | None:intergenic |
| AGTACGGGTTTCCTTAGCAA+TGG | 0.542466 | 4:+62470758 | None:intergenic |
| AGCTTAAACTCCCATTGCTA+AGG | 0.549995 | 4:-62470769 | MsG0480021663.01.T01:CDS |
| GCCTTGCCAATGGTCAGTGG+TGG | 0.561667 | 4:+62471072 | None:intergenic |
| TGGATTAGAAGCCAGTGCCT+TGG | 0.563915 | 4:+62470715 | None:intergenic |
| ACCTAGCTGTTGAGATAACA+TGG | 0.576355 | 4:-62470811 | MsG0480021663.01.T01:CDS |
| GTTATATCCAAGTCAGTGGA+TGG | 0.578815 | 4:+62470992 | None:intergenic |
| GTTCACCCACCACTGACCAT+TGG | 0.585098 | 4:-62471078 | MsG0480021663.01.T01:CDS |
| GATGATAGAAAATCTATGGC+TGG | 0.587157 | 4:-62470935 | MsG0480021663.01.T01:CDS |
| GCCTGTTATATCCAAGTCAG+TGG | 0.591147 | 4:+62470988 | None:intergenic |
| TATGCCTTGCCAATGGTCAG+TGG | 0.610369 | 4:+62471069 | None:intergenic |
| CCTTGCCAATGGTCAGTGGT+GGG | 0.619616 | 4:+62471073 | None:intergenic |
| GTGTGATAACTTAAGTGCCA+AGG | 0.625402 | 4:-62470732 | MsG0480021663.01.T01:CDS |
| CCCACCACTGACCATTGGCA+AGG | 0.643075 | 4:-62471073 | MsG0480021663.01.T01:CDS |
| TCCATGTTATCTCAACAGCT+AGG | 0.648475 | 4:+62470810 | None:intergenic |
| AGAATCTTGAGATATCTCCA+AGG | 0.682025 | 4:-62471043 | MsG0480021663.01.T01:CDS |
| AAGAGACAAGTGTTTCACCA+AGG | 0.731912 | 4:+62470900 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTTGATGATAGAAAATCTA+TGG | - | Chr4:62470792-62470811 | MsG0480021663.01.T01:CDS | 25.0% |
| TAAGTTATCACACCATAGTA+CGG | + | Chr4:62470992-62471011 | None:intergenic | 30.0% | |
| ! | TTTTCTATCATCAACACTAG+TGG | + | Chr4:62470788-62470807 | None:intergenic | 30.0% |
| !!! | TACTACCTTTTGTTTTCTTG+AGG | + | Chr4:62470860-62470879 | None:intergenic | 30.0% |
| AAGTTATCACACCATAGTAC+GGG | + | Chr4:62470991-62471010 | None:intergenic | 35.0% | |
| AGAATCTTGAGATATCTCCA+AGG | - | Chr4:62470688-62470707 | MsG0480021663.01.T01:CDS | 35.0% | |
| GATGATAGAAAATCTATGGC+TGG | - | Chr4:62470796-62470815 | MsG0480021663.01.T01:CDS | 35.0% | |
| TTGGTCCTCAAGAAAACAAA+AGG | - | Chr4:62470852-62470871 | MsG0480021663.01.T01:CDS | 35.0% | |
| ! | TTCTTTTTATGCCTTGCCAA+TGG | + | Chr4:62470672-62470691 | None:intergenic | 35.0% |
| AAGAGACAAGTGTTTCACCA+AGG | + | Chr4:62470834-62470853 | None:intergenic | 40.0% | |
| ACCTAGCTGTTGAGATAACA+TGG | - | Chr4:62470920-62470939 | MsG0480021663.01.T01:CDS | 40.0% | |
| AGCTTAAACTCCCATTGCTA+AGG | - | Chr4:62470962-62470981 | MsG0480021663.01.T01:CDS | 40.0% | |
| GATGTGCAAGCAATGATTGA+TGG | + | Chr4:62470716-62470735 | None:intergenic | 40.0% | |
| GTGTGATAACTTAAGTGCCA+AGG | - | Chr4:62470999-62471018 | MsG0480021663.01.T01:CDS | 40.0% | |
| GTTATATCCAAGTCAGTGGA+TGG | + | Chr4:62470742-62470761 | None:intergenic | 40.0% | |
| TCCATGTTATCTCAACAGCT+AGG | + | Chr4:62470924-62470943 | None:intergenic | 40.0% | |
| TTGGTGAAACACTTGTCTCT+TGG | - | Chr4:62470833-62470852 | MsG0480021663.01.T01:CDS | 40.0% | |
| !! | TCCACTGACTTGGATATAAC+AGG | - | Chr4:62470742-62470761 | MsG0480021663.01.T01:CDS | 40.0% |
| AGGCTTCTCTGATGCAGATT+GGG | - | Chr4:62470762-62470781 | MsG0480021663.01.T01:CDS | 45.0% | |
| AGTACGGGTTTCCTTAGCAA+TGG | + | Chr4:62470976-62470995 | None:intergenic | 45.0% | |
| GCCTGTTATATCCAAGTCAG+TGG | + | Chr4:62470746-62470765 | None:intergenic | 45.0% | |
| GTACGGGTTTCCTTAGCAAT+GGG | + | Chr4:62470975-62470994 | None:intergenic | 45.0% | |
| TAACTTAAGTGCCAAGGCAC+TGG | - | Chr4:62471005-62471024 | MsG0480021663.01.T01:CDS | 45.0% | |
| TGCTAAGGAAACCCGTACTA+TGG | - | Chr4:62470977-62470996 | MsG0480021663.01.T01:CDS | 45.0% | |
| TTAGAACGAGCATGCAGAAC+TGG | + | Chr4:62471039-62471058 | None:intergenic | 45.0% | |
| ! | CATCAAACCATCCACTGACT+TGG | - | Chr4:62470732-62470751 | MsG0480021663.01.T01:CDS | 45.0% |
| !! | AGCAATGATTGATGGTGCCT+TGG | + | Chr4:62470708-62470727 | None:intergenic | 45.0% |
| CAGGCTTCTCTGATGCAGAT+TGG | - | Chr4:62470761-62470780 | MsG0480021663.01.T01:CDS | 50.0% | |
| GCTGGTCAATGTGTGTTCCT+TGG | - | Chr4:62470814-62470833 | MsG0480021663.01.T01:CDS | 50.0% | |
| TATGCCTTGCCAATGGTCAG+TGG | + | Chr4:62470665-62470684 | None:intergenic | 50.0% | |
| ! | TGGATTAGAAGCCAGTGCCT+TGG | + | Chr4:62471019-62471038 | None:intergenic | 50.0% |
| CCTTGCCAATGGTCAGTGGT+GGG | + | Chr4:62470661-62470680 | None:intergenic | 55.0% | |
| GTTCACCCACCACTGACCAT+TGG | - | Chr4:62470653-62470672 | MsG0480021663.01.T01:CDS | 55.0% | |
| CCCACCACTGACCATTGGCA+AGG | - | Chr4:62470658-62470677 | MsG0480021663.01.T01:CDS | 60.0% | |
| GCCTTGCCAATGGTCAGTGG+TGG | + | Chr4:62470662-62470681 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 62470649 | 62471104 | 62470649 | ID=MsG0480021663.01;Name=MsG0480021663.01 |
| Chr4 | mRNA | 62470649 | 62471104 | 62470649 | ID=MsG0480021663.01.T01;Parent=MsG0480021663.01;Name=MsG0480021663.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|151 |
| Chr4 | exon | 62470649 | 62471104 | 62470649 | ID=MsG0480021663.01.T01:exon:1695;Parent=MsG0480021663.01.T01 |
| Chr4 | CDS | 62470649 | 62471104 | 62470649 | ID=MsG0480021663.01.T01:cds;Parent=MsG0480021663.01.T01 |
| Gene Sequence |
| Protein sequence |
