AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021694.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021694.01.T01 MTR_4g075280 92.000 200 6 1 1 200 1 190 2.15e-131 367
MsG0480021694.01.T01 MTR_3g109700 43.357 143 67 4 60 200 63 193 4.66e-31 112
MsG0480021694.01.T01 MTR_3g109700 44.444 108 57 2 57 163 60 165 9.00e-26 98.2
MsG0480021694.01.T01 MTR_3g109700 44.860 107 56 2 57 162 60 164 2.26e-25 97.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021694.01.T01 AT5G16870 65.500 200 38 5 1 200 1 169 7.06e-82 241
MsG0480021694.01.T01 AT3G03010 65.054 186 42 3 17 200 15 179 1.31e-75 225
MsG0480021694.01.T01 AT3G03010 65.054 186 42 3 17 200 15 179 1.31e-75 225
MsG0480021694.01.T01 AT3G03010 65.054 186 42 3 17 200 15 179 1.31e-75 225
MsG0480021694.01.T01 AT3G03010 61.719 128 36 2 17 142 15 131 3.36e-45 147
MsG0480021694.01.T01 AT3G03010 61.719 128 36 2 17 142 15 131 3.36e-45 147
MsG0480021694.01.T01 AT4G32900 44.882 127 59 2 75 200 77 193 9.91e-31 111
MsG0480021694.01.T01 AT4G32900 45.556 90 49 0 75 164 77 166 2.58e-24 94.4
MsG0480021694.01.T01 AT4G32900 45.556 90 49 0 75 164 77 166 2.58e-24 94.4
MsG0480021694.01.T01 AT4G32900 46.875 64 34 0 75 138 77 140 5.28e-16 72.0

Find 66 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAAGTTGGTCTTTGATTT+TGG 0.042547 4:-63020003 None:intergenic
TGTTAGAAATGATCTTAAAA+TGG 0.178309 4:+63020156 MsG0480021694.01.T01:CDS
GATTGCTCCAAGTGAGTATT+TGG 0.196840 4:+63022333 MsG0480021694.01.T01:CDS
CTTGCTTTGGGTTACTTCAT+TGG 0.226259 4:+63019873 MsG0480021694.01.T01:CDS
GTCATGGTTGAGTGCAATTT+TGG 0.265900 4:+63019836 MsG0480021694.01.T01:CDS
AACATTGGGTCTCTATAAAA+AGG 0.277970 4:+63020732 MsG0480021694.01.T01:CDS
AAAACAATCATGGTACTTCT+AGG 0.320711 4:-63023442 None:intergenic
TGGTGCTGGTTATCTTGCTT+TGG 0.338108 4:+63019860 MsG0480021694.01.T01:CDS
AAAATTTGAATAGGAACTTA+AGG 0.340846 4:-63023493 None:intergenic
GGTGCTGGTTATCTTGCTTT+GGG 0.344764 4:+63019861 MsG0480021694.01.T01:CDS
GTATCCTCACCCACAATAAC+TGG 0.345976 4:+63023348 MsG0480021694.01.T01:three_prime_UTR
CATTACCTATTTAAAGCCTT+TGG 0.351201 4:-63020769 None:intergenic
ACTTCTAGGAACATAAGTTT+TGG 0.353454 4:-63023428 None:intergenic
GAGAATTGCCATCACCGTTC+TGG 0.354128 4:-63022891 None:intergenic
CAACATTTAAAACAATGAAT+TGG 0.359514 4:-63023400 None:intergenic
GAGCTCCAAAGGCTTTAAAT+AGG 0.364463 4:+63020764 MsG0480021694.01.T01:CDS
TGATCCTCACCTGATTGAAA+TGG 0.371048 4:+63019809 MsG0480021694.01.T01:exon
GTTAGAAATGATCTTAAAAT+GGG 0.382687 4:+63020157 MsG0480021694.01.T01:CDS
ATGACATTTCCATTTCAATC+AGG 0.396124 4:-63019818 None:intergenic
AATCAGGTGAGGATCAGAAT+TGG 0.401464 4:-63019802 None:intergenic
TAATAGTTGAAAATTTGAAT+AGG 0.410815 4:-63023502 None:intergenic
TCTCATGATTCCAGTTATTG+TGG 0.420375 4:-63023358 None:intergenic
AATCAATTAACAAGAAGATA+AGG 0.432485 4:-63022362 None:intergenic
ATCAGGTGAGGATCAGAATT+GGG 0.446594 4:-63019801 None:intergenic
AAATAATGAAACTCCTTCAC+TGG 0.455126 4:-63023154 None:intergenic
ATCAATTAACAAGAAGATAA+GGG 0.457994 4:-63022361 None:intergenic
ACCTCATCTACCACTTCAAC+AGG 0.458269 4:-63023100 None:intergenic
CATTACTTCGTTGGTATCCC+GGG 0.461368 4:+63023236 MsG0480021694.01.T01:three_prime_UTR
GATGATGAAAACTTCTTGTT+GGG 0.470354 4:+63023326 MsG0480021694.01.T01:three_prime_UTR
ACATTACTTCGTTGGTATCC+CGG 0.470605 4:+63023235 MsG0480021694.01.T01:three_prime_UTR
TATTTAAAGCCTTTGGAGCT+CGG 0.471485 4:-63020762 None:intergenic
TCATTATTTACCATGAGGTC+GGG 0.485530 4:+63023168 MsG0480021694.01.T01:three_prime_UTR
TGATGATGAAAACTTCTTGT+TGG 0.486354 4:+63023325 MsG0480021694.01.T01:three_prime_UTR
CTCATGATTCCAGTTATTGT+GGG 0.486926 4:-63023357 None:intergenic
TTCATTATTTACCATGAGGT+CGG 0.496836 4:+63023167 MsG0480021694.01.T01:three_prime_UTR
AATGATCTTAAAATGGGTAA+AGG 0.501457 4:+63020163 MsG0480021694.01.T01:CDS
AGAACGGTGATGGCAATTCT+CGG 0.504068 4:+63022893 MsG0480021694.01.T01:CDS
TATGCAGAACATTACTTCGT+TGG 0.506718 4:+63023227 MsG0480021694.01.T01:three_prime_UTR
GATTCCAGTTATTGTGGGTG+AGG 0.511891 4:-63023352 None:intergenic
GAAAGATTGCAGCTCAATGC+AGG 0.516623 4:+63020185 MsG0480021694.01.T01:CDS
GGAGATGTCTGCACAGCCTA+AGG 0.521064 4:+63020875 MsG0480021694.01.T01:CDS
TGAAAGTGAAGAAGATATGC+TGG 0.522958 4:+63020911 MsG0480021694.01.T01:CDS
TCTAGATTCCAGAACGGTGA+TGG 0.534748 4:+63022883 MsG0480021694.01.T01:intron
ACTCATATCACAATTGATGC+TGG 0.549551 4:+63022301 MsG0480021694.01.T01:CDS
GGTTCTTTACCGAGCTCCAA+AGG 0.550602 4:+63020753 MsG0480021694.01.T01:CDS
GTACAACCAGAAAACAATCA+TGG 0.563501 4:-63023452 None:intergenic
GCATCAATTGTGATATGAGT+AGG 0.570579 4:-63022298 None:intergenic
CACTCATCAACCCGACCTCA+TGG 0.574056 4:-63023178 None:intergenic
GAAGTGGTAGATGAGGTAAC+AGG 0.582781 4:+63023106 MsG0480021694.01.T01:CDS
CAAGCTGTTCAACCTCAAGT+TGG 0.585211 4:-63020016 None:intergenic
AAAACATGCAAAATCTTCCC+GGG 0.589723 4:-63023253 None:intergenic
ACCTGTTGAAGTGGTAGATG+AGG 0.590585 4:+63023099 MsG0480021694.01.T01:CDS
GGTAGTTCCAAATACTCACT+TGG 0.591169 4:-63022340 None:intergenic
ATTTCCATTTCAATCAGGTG+AGG 0.600422 4:-63019813 None:intergenic
TCTATAATCATGTCCAGTGA+AGG 0.602310 4:+63023141 MsG0480021694.01.T01:exon
TGAGACTAAGTAAATACAGA+AGG 0.610301 4:+63023376 MsG0480021694.01.T01:three_prime_UTR
GACTAACTACAATTCTTGTG+TGG 0.615450 4:-63023280 None:intergenic
TAACTACAATTCTTGTGTGG+AGG 0.621352 4:-63023277 None:intergenic
ATGTGCAGGACCTGTTGAAG+TGG 0.631821 4:+63023090 MsG0480021694.01.T01:intron
TCTGCATAACGTTTGATGTG+TGG 0.639787 4:-63023212 None:intergenic
GAGTTTCATTATTTACCATG+AGG 0.647378 4:+63023163 MsG0480021694.01.T01:three_prime_UTR
GTTTCTTCTAGATTCCAGAA+CGG 0.652178 4:+63022877 MsG0480021694.01.T01:intron
GTGGTAGATGAGGTAACAGG+TGG 0.666083 4:+63023109 MsG0480021694.01.T01:CDS
TGATTGAAATGGAAATGTCA+TGG 0.672695 4:+63019820 MsG0480021694.01.T01:exon
GGGTTGATGAGTGTTCATGA+AGG 0.678743 4:+63023188 MsG0480021694.01.T01:three_prime_UTR
CAAAATCAAAGACCAACTTG+AGG 0.704651 4:+63020004 MsG0480021694.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATCCTTTTAATTTATT+AGG + Chr4:63021551-63021570 MsG0480021694.01.T01:intron 10.0%
!!! TAAATATTGTTTGATTTTTA+GGG + Chr4:63020943-63020962 MsG0480021694.01.T01:intron 10.0%
!! ATCCAGAAAAAAAATATAAA+CGG + Chr4:63020667-63020686 MsG0480021694.01.T01:intron 15.0%
!! ATTAATCAAATGATATCATT+AGG + Chr4:63021582-63021601 MsG0480021694.01.T01:intron 15.0%
!! TAATAGTTGAAAATTTGAAT+AGG - Chr4:63023505-63023524 None:intergenic 15.0%
!! TTAATCAATGAAAGTAAAAA+GGG + Chr4:63021241-63021260 MsG0480021694.01.T01:intron 15.0%
!! TTTAATCAATGAAAGTAAAA+AGG + Chr4:63021240-63021259 MsG0480021694.01.T01:intron 15.0%
!!! AATTACTAGCTTTATTTTAA+TGG + Chr4:63020096-63020115 MsG0480021694.01.T01:intron 15.0%
!!! AGGATATTTTTCTTTTAATT+TGG - Chr4:63021540-63021559 None:intergenic 15.0%
!!! ATTACTAGCTTTATTTTAAT+GGG + Chr4:63020097-63020116 MsG0480021694.01.T01:intron 15.0%
!!! GTAAATATTGTTTGATTTTT+AGG + Chr4:63020942-63020961 MsG0480021694.01.T01:intron 15.0%
!!! TTTACTTTCATTGATTAAAT+AGG - Chr4:63021239-63021258 None:intergenic 15.0%
!!! TTTTTATTCTAATAACACTA+TGG + Chr4:63021808-63021827 MsG0480021694.01.T01:intron 15.0%
!!! TTTTTTTTAATGCTACTATA+AGG - Chr4:63022442-63022461 None:intergenic 15.0%
!! AAAATTTGAATAGGAACTTA+AGG - Chr4:63023496-63023515 None:intergenic 20.0%
!! AAAGAGAACATAGTTAATAT+TGG - Chr4:63020978-63020997 None:intergenic 20.0%
!! AACTTATTTGATATATTGAC+TGG + Chr4:63022066-63022085 MsG0480021694.01.T01:intron 20.0%
!! AATCAATTAACAAGAAGATA+AGG - Chr4:63022365-63022384 None:intergenic 20.0%
!! ATCAATTAACAAGAAGATAA+GGG - Chr4:63022364-63022383 None:intergenic 20.0%
!! CTAAGCAATAATAATCTATT+TGG - Chr4:63020523-63020542 None:intergenic 20.0%
!! GTTAGAAATGATCTTAAAAT+GGG + Chr4:63020157-63020176 MsG0480021694.01.T01:CDS 20.0%
!! TGTTAGAAATGATCTTAAAA+TGG + Chr4:63020156-63020175 MsG0480021694.01.T01:CDS 20.0%
!! TTATATTCAAAGTTGAAACT+TGG + Chr4:63021315-63021334 MsG0480021694.01.T01:intron 20.0%
!! TTTCAGCCTAATAAATTAAA+AGG - Chr4:63021560-63021579 None:intergenic 20.0%
!!! AAAAAAGTATAGCATAAACA+AGG + Chr4:63021965-63021984 MsG0480021694.01.T01:intron 20.0%
!!! AAAAGCTAAGTTGATATAAA+TGG - Chr4:63022943-63022962 None:intergenic 20.0%
!!! AACGTCTTTTATTATTGTAA+AGG + Chr4:63021862-63021881 MsG0480021694.01.T01:intron 20.0%
!!! CAACATTTAAAACAATGAAT+TGG - Chr4:63023403-63023422 None:intergenic 20.0%
!!! TTTATTTTCTATTTTTCTCC+TGG - Chr4:63021140-63021159 None:intergenic 20.0%
! AAACTTCAATGTATAAGATG+AGG - Chr4:63022614-63022633 None:intergenic 25.0%
! AATGATCTTAAAATGGGTAA+AGG + Chr4:63020163-63020182 MsG0480021694.01.T01:CDS 25.0%
! AATGTATAAGATGAGGATAA+TGG - Chr4:63022607-63022626 None:intergenic 25.0%
! ACAACAAGTATCTACAAAAA+AGG - Chr4:63020139-63020158 None:intergenic 25.0%
! ACACCAAATAAACACTATTA+TGG + Chr4:63021067-63021086 MsG0480021694.01.T01:intron 25.0%
! AGGTAAAATTTCCAATTATG+TGG - Chr4:63022403-63022422 None:intergenic 25.0%
! ATAAATGGTCATTCTATTAG+AGG - Chr4:63022928-63022947 None:intergenic 25.0%
! ATATCATTAGGATTATGTCT+AGG + Chr4:63021594-63021613 MsG0480021694.01.T01:intron 25.0%
! ATGCAAATTGTCATGTATAT+TGG + Chr4:63022482-63022501 MsG0480021694.01.T01:intron 25.0%
! ATTTCAGATCTAATTGTACA+TGG - Chr4:63022539-63022558 None:intergenic 25.0%
! CAACCATAATAGTGTTTATT+TGG - Chr4:63021073-63021092 None:intergenic 25.0%
! CAACGAAAAAACAAAAGAAA+AGG - Chr4:63022775-63022794 None:intergenic 25.0%
! CTTTAAATAGGTAATGTCAT+TGG + Chr4:63020776-63020795 MsG0480021694.01.T01:intron 25.0%
! GCAATAATAATCTATTTGGA+AGG - Chr4:63020519-63020538 None:intergenic 25.0%
! GCTTAAATATCTCCAAAATT+AGG + Chr4:63022145-63022164 MsG0480021694.01.T01:intron 25.0%
! TCAAGTATGTCAATCTAATT+AGG + Chr4:63020468-63020487 MsG0480021694.01.T01:intron 25.0%
! TTTGTATCTGTATGATATGA+AGG + Chr4:63022806-63022825 MsG0480021694.01.T01:intron 25.0%
!! AACATGAAAGAACCTAATTT+TGG - Chr4:63022160-63022179 None:intergenic 25.0%
!! AATGAGTTGAAAACAGTTTA+TGG + Chr4:63020376-63020395 MsG0480021694.01.T01:intron 25.0%
!! AGGCAAAAAATAGTAGATTT+TGG + Chr4:63021181-63021200 MsG0480021694.01.T01:intron 25.0%
!! AGGGATGTATATTTCATTTT+AGG - Chr4:63022584-63022603 None:intergenic 25.0%
!! ATAAGCTCAGTTAGATATTT+TGG + Chr4:63021904-63021923 MsG0480021694.01.T01:intron 25.0%
!! GGATGTATATTTCATTTTAG+GGG - Chr4:63022582-63022601 None:intergenic 25.0%
!! GGGATGTATATTTCATTTTA+GGG - Chr4:63022583-63022602 None:intergenic 25.0%
!! TACAAAAGCTATCTTTAGAA+AGG - Chr4:63022226-63022245 None:intergenic 25.0%
!!! GACCGTTTATATTTTTTTTC+TGG - Chr4:63020672-63020691 None:intergenic 25.0%
!!! TATTCTCGAAGATTTTAAGA+TGG + Chr4:63020043-63020062 MsG0480021694.01.T01:CDS 25.0%
AAAAACATGCAAAATCTTCC+CGG - Chr4:63023257-63023276 None:intergenic 30.0%
AAAACAATCATGGTACTTCT+AGG - Chr4:63023445-63023464 None:intergenic 30.0%
AAAATAGTCCAACCTATTAG+AGG - Chr4:63022023-63022042 None:intergenic 30.0%
AAATAATGAAACTCCTTCAC+TGG - Chr4:63023157-63023176 None:intergenic 30.0%
AAATTGTCATGTATATTGGC+TGG + Chr4:63022486-63022505 MsG0480021694.01.T01:intron 30.0%
AACATTGGGTCTCTATAAAA+AGG + Chr4:63020732-63020751 MsG0480021694.01.T01:CDS 30.0%
ACTAACACTGCAATCATATT+AGG + Chr4:63021357-63021376 MsG0480021694.01.T01:intron 30.0%
ATACGTAGCAGTGTATAATT+TGG + Chr4:63021442-63021461 MsG0480021694.01.T01:intron 30.0%
ATACTTGTAGAAACTGTCTA+AGG + Chr4:63021213-63021232 MsG0480021694.01.T01:intron 30.0%
ATGACATTTCCATTTCAATC+AGG - Chr4:63019821-63019840 None:intergenic 30.0%
CTATATGCAATGAAAAGCAA+GGG + Chr4:63021690-63021709 MsG0480021694.01.T01:intron 30.0%
CTGTTTCAAAAGAAATGTTG+TGG + Chr4:63021388-63021407 MsG0480021694.01.T01:intron 30.0%
GAGTTTCATTATTTACCATG+AGG + Chr4:63023163-63023182 MsG0480021694.01.T01:three_prime_UTR 30.0%
GATGATGAAAACTTCTTGTT+GGG + Chr4:63023326-63023345 MsG0480021694.01.T01:three_prime_UTR 30.0%
TACGTAGCAGTGTATAATTT+GGG + Chr4:63021443-63021462 MsG0480021694.01.T01:intron 30.0%
TGAGACTAAGTAAATACAGA+AGG + Chr4:63023376-63023395 MsG0480021694.01.T01:three_prime_UTR 30.0%
TGATTGAAATGGAAATGTCA+TGG + Chr4:63019820-63019839 MsG0480021694.01.T01:exon 30.0%
TGTAATGTTCTCCACATAAT+TGG + Chr4:63022389-63022408 MsG0480021694.01.T01:intron 30.0%
TTATTGTAAAGGAACTCTTG+TGG + Chr4:63021873-63021892 MsG0480021694.01.T01:intron 30.0%
TTCATTATTTACCATGAGGT+CGG + Chr4:63023167-63023186 MsG0480021694.01.T01:three_prime_UTR 30.0%
TTCTGGATTAAAAACAGAAC+AGG - Chr4:63020655-63020674 None:intergenic 30.0%
TTGTTTGGATAAGCACTTAA+TGG + Chr4:63020238-63020257 MsG0480021694.01.T01:intron 30.0%
! AATTGTCATGTATATTGGCT+GGG + Chr4:63022487-63022506 MsG0480021694.01.T01:intron 30.0%
! AGTTTTTGAACATGATGTCA+TGG + Chr4:63022983-63023002 MsG0480021694.01.T01:intron 30.0%
! ATCCTTTTGACATTTGAACT+AGG + Chr4:63021161-63021180 MsG0480021694.01.T01:intron 30.0%
! ATTTTGTAGTCATGCAACAT+TGG + Chr4:63020717-63020736 MsG0480021694.01.T01:intron 30.0%
! CATTACCTATTTAAAGCCTT+TGG - Chr4:63020772-63020791 None:intergenic 30.0%
! GGTGTTAATTTGATCTTGTT+TGG + Chr4:63020223-63020242 MsG0480021694.01.T01:intron 30.0%
! GTTTTTGAACATGATGTCAT+GGG + Chr4:63022984-63023003 MsG0480021694.01.T01:intron 30.0%
! TGATGATGAAAACTTCTTGT+TGG + Chr4:63023325-63023344 MsG0480021694.01.T01:three_prime_UTR 30.0%
! TTTTGTAGTCATGCAACATT+GGG + Chr4:63020718-63020737 MsG0480021694.01.T01:intron 30.0%
!! AACACTATTATGGTTGCTTT+TGG + Chr4:63021077-63021096 MsG0480021694.01.T01:intron 30.0%
!! ACTTCTAGGAACATAAGTTT+TGG - Chr4:63023431-63023450 None:intergenic 30.0%
!! TATAAGATGAGGATAATGGA+GGG - Chr4:63022603-63022622 None:intergenic 30.0%
!! TCAATTTTGACAACAACCTT+AGG - Chr4:63020894-63020913 None:intergenic 30.0%
!! TTGGGCTTTAAACAATGATT+GGG + Chr4:63021461-63021480 MsG0480021694.01.T01:intron 30.0%
!! TTTGGGCTTTAAACAATGAT+TGG + Chr4:63021460-63021479 MsG0480021694.01.T01:intron 30.0%
!!! CACAGTTTTAGACAATACAA+AGG - Chr4:63022700-63022719 None:intergenic 30.0%
!!! TAGATATTTTGGTAATCTGC+CGG + Chr4:63021915-63021934 MsG0480021694.01.T01:intron 30.0%
!!! TCAAGTTGGTCTTTGATTTT+GGG - Chr4:63020005-63020024 None:intergenic 30.0%
AAAACATGCAAAATCTTCCC+GGG - Chr4:63023256-63023275 None:intergenic 35.0%
AAGGCAGTAAAATAACACAC+AGG - Chr4:63022423-63022442 None:intergenic 35.0%
ACTCATATCACAATTGATGC+TGG + Chr4:63022301-63022320 MsG0480021694.01.T01:CDS 35.0%
ATACTGTCAGAACCTCTAAT+AGG + Chr4:63022008-63022027 MsG0480021694.01.T01:intron 35.0%
ATTTAAGCGAACTATGGAAG+AGG - Chr4:63022133-63022152 None:intergenic 35.0%
ATTTCCATTTCAATCAGGTG+AGG - Chr4:63019816-63019835 None:intergenic 35.0%
CAAAATCAAAGACCAACTTG+AGG + Chr4:63020004-63020023 MsG0480021694.01.T01:CDS 35.0%
CAATGCAAGACATGAAACTT+AGG + Chr4:63021278-63021297 MsG0480021694.01.T01:intron 35.0%
CTCATGATTCCAGTTATTGT+GGG - Chr4:63023360-63023379 None:intergenic 35.0%
CTGTTGTTCTATAAGCTATC+TGG + Chr4:63020342-63020361 MsG0480021694.01.T01:intron 35.0%
CTTTAAACAATGATTGGGCA+GGG + Chr4:63021466-63021485 MsG0480021694.01.T01:intron 35.0%
GACTAACTACAATTCTTGTG+TGG - Chr4:63023283-63023302 None:intergenic 35.0%
GCATCAATTGTGATATGAGT+AGG - Chr4:63022301-63022320 None:intergenic 35.0%
GCTATATGCAATGAAAAGCA+AGG + Chr4:63021689-63021708 MsG0480021694.01.T01:intron 35.0%
GGAGATATTTAAGCGAACTA+TGG - Chr4:63022139-63022158 None:intergenic 35.0%
GTACAACCAGAAAACAATCA+TGG - Chr4:63023455-63023474 None:intergenic 35.0%
GTTTCTTCTAGATTCCAGAA+CGG + Chr4:63022877-63022896 MsG0480021694.01.T01:intron 35.0%
TAAAAAGAAGTTCAGTCCAC+CGG - Chr4:63023037-63023056 None:intergenic 35.0%
TAACTACAATTCTTGTGTGG+AGG - Chr4:63023280-63023299 None:intergenic 35.0%
TATGCAGAACATTACTTCGT+TGG + Chr4:63023227-63023246 MsG0480021694.01.T01:three_prime_UTR 35.0%
TATTTGTTTGCGAGAGCTTA+TGG - Chr4:63020420-63020439 None:intergenic 35.0%
TCATTATTTACCATGAGGTC+GGG + Chr4:63023168-63023187 MsG0480021694.01.T01:three_prime_UTR 35.0%
TCTATAATCATGTCCAGTGA+AGG + Chr4:63023141-63023160 MsG0480021694.01.T01:exon 35.0%
TCTCATGATTCCAGTTATTG+TGG - Chr4:63023361-63023380 None:intergenic 35.0%
TGAAAGTGAAGAAGATATGC+TGG + Chr4:63020911-63020930 MsG0480021694.01.T01:CDS 35.0%
TGCAGGTATGTAATCTTTGT+TGG + Chr4:63020202-63020221 MsG0480021694.01.T01:intron 35.0%
TGCCTAGTTCAAATGTCAAA+AGG - Chr4:63021166-63021185 None:intergenic 35.0%
TTAACATGATGTTCATGTGC+AGG + Chr4:63023076-63023095 MsG0480021694.01.T01:intron 35.0%
! AACAGAACAGTTCGTCTTTT+AGG - Chr4:63021749-63021768 None:intergenic 35.0%
! ACAGAACAGTTCGTCTTTTA+GGG - Chr4:63021748-63021767 None:intergenic 35.0%
! AGGATCAGAATTGGGATTTT+AGG - Chr4:63019796-63019815 None:intergenic 35.0%
! GGATCAGAATTGGGATTTTA+GGG - Chr4:63019795-63019814 None:intergenic 35.0%
! GTATAAGATGAGGATAATGG+AGG - Chr4:63022604-63022623 None:intergenic 35.0%
! TTTTCTTCAGAAAGCAAGCA+TGG - Chr4:63022660-63022679 None:intergenic 35.0%
!! ATACTGTCTTTTGCTGTGTT+AGG + Chr4:63022252-63022271 MsG0480021694.01.T01:intron 35.0%
!! GAAGTACCATGATTGTTTTC+TGG + Chr4:63023446-63023465 MsG0480021694.01.T01:three_prime_UTR 35.0%
!! GTTTTTCAATGTCAATGTGC+AGG + Chr4:63020850-63020869 MsG0480021694.01.T01:intron 35.0%
!! TATTTAAAGCCTTTGGAGCT+CGG - Chr4:63020765-63020784 None:intergenic 35.0%
!! TCTTTTGCTGTGTTAGGAAA+GGG + Chr4:63022258-63022277 MsG0480021694.01.T01:intron 35.0%
!!! ACTATTATGGTTGCTTTTGG+TGG + Chr4:63021080-63021099 MsG0480021694.01.T01:intron 35.0%
!!! AGATATTTTGGTAATCTGCC+GGG + Chr4:63021916-63021935 MsG0480021694.01.T01:intron 35.0%
!!! CTATTATGGTTGCTTTTGGT+GGG + Chr4:63021081-63021100 MsG0480021694.01.T01:intron 35.0%
!!! CTCAAGTTGGTCTTTGATTT+TGG - Chr4:63020006-63020025 None:intergenic 35.0%
AATCAGGTGAGGATCAGAAT+TGG - Chr4:63019805-63019824 None:intergenic 40.0%
AATGAAAAGCAAGGGAGAGT+AGG + Chr4:63021698-63021717 MsG0480021694.01.T01:intron 40.0%
ACATACTTGAGCTTACCTAC+AGG - Chr4:63020458-63020477 None:intergenic 40.0%
ACATTACTTCGTTGGTATCC+CGG + Chr4:63023235-63023254 MsG0480021694.01.T01:three_prime_UTR 40.0%
ATCAGGTGAGGATCAGAATT+GGG - Chr4:63019804-63019823 None:intergenic 40.0%
CATTAGGATTATGTCTAGGC+TGG + Chr4:63021598-63021617 MsG0480021694.01.T01:intron 40.0%
GATTGCTCCAAGTGAGTATT+TGG + Chr4:63022333-63022352 MsG0480021694.01.T01:CDS 40.0%
GGTAGTTCCAAATACTCACT+TGG - Chr4:63022343-63022362 None:intergenic 40.0%
GTGGCTGATTAATCATTGTG+AGG + Chr4:63021407-63021426 MsG0480021694.01.T01:intron 40.0%
TAGCCTAGTGATTTACATCC+CGG - Chr4:63021937-63021956 None:intergenic 40.0%
TCTGCATAACGTTTGATGTG+TGG - Chr4:63023215-63023234 None:intergenic 40.0%
TGATCCTCACCTGATTGAAA+TGG + Chr4:63019809-63019828 MsG0480021694.01.T01:exon 40.0%
TGCTTAGATTGCGACATTCT+AGG + Chr4:63020536-63020555 MsG0480021694.01.T01:intron 40.0%
TGTAAAGGAACTCTTGTGGA+AGG + Chr4:63021877-63021896 MsG0480021694.01.T01:intron 40.0%
TGTCAGAACCTCTAATAGGT+TGG + Chr4:63022012-63022031 MsG0480021694.01.T01:intron 40.0%
TTCAATGTCAATGTGCAGGT+GGG + Chr4:63020854-63020873 MsG0480021694.01.T01:intron 40.0%
TTCAGAAAGCAAGCATGGAT+AGG - Chr4:63022655-63022674 None:intergenic 40.0%
TTTCAATGTCAATGTGCAGG+TGG + Chr4:63020853-63020872 MsG0480021694.01.T01:intron 40.0%
! ATGTCATTGGTTGAGCTTGT+TGG + Chr4:63020789-63020808 MsG0480021694.01.T01:intron 40.0%
! CTTGCTTTGGGTTACTTCAT+TGG + Chr4:63019873-63019892 MsG0480021694.01.T01:CDS 40.0%
! GCTTTAAACAATGATTGGGC+AGG + Chr4:63021465-63021484 MsG0480021694.01.T01:intron 40.0%
! GTCATGGTTGAGTGCAATTT+TGG + Chr4:63019836-63019855 MsG0480021694.01.T01:CDS 40.0%
! TGTTTTTCTATCCTCTTCCC+CGG + Chr4:63019767-63019786 MsG0480021694.01.T01:five_prime_UTR 40.0%
!! CATACTTGCTTGCTGTACAT+TGG + Chr4:63021022-63021041 MsG0480021694.01.T01:intron 40.0%
!! GAGCTCCAAAGGCTTTAAAT+AGG + Chr4:63020764-63020783 MsG0480021694.01.T01:CDS 40.0%
!! GTCTTTTGCTGTGTTAGGAA+AGG + Chr4:63022257-63022276 MsG0480021694.01.T01:intron 40.0%
!! TGGATAGGCATTTTCAAGCA+TGG - Chr4:63022640-63022659 None:intergenic 40.0%
!! TGGTTGAGTGCAATTTTGGT+TGG + Chr4:63019840-63019859 MsG0480021694.01.T01:CDS 40.0%
!!! AATTGGGATTTTAGGGTTCC+GGG - Chr4:63019788-63019807 None:intergenic 40.0%
!!! GAATTGGGATTTTAGGGTTC+CGG - Chr4:63019789-63019808 None:intergenic 40.0%
AAACAATGATTGGGCAGGGA+GGG + Chr4:63021470-63021489 MsG0480021694.01.T01:intron 45.0%
ACCTCATCTACCACTTCAAC+AGG - Chr4:63023103-63023122 None:intergenic 45.0%
ACCTGTTGAAGTGGTAGATG+AGG + Chr4:63023099-63023118 MsG0480021694.01.T01:CDS 45.0%
ACTAGAGAAGTTCAGCAACC+AGG + Chr4:63021119-63021138 MsG0480021694.01.T01:intron 45.0%
CAAGCTGTTCAACCTCAAGT+TGG - Chr4:63020019-63020038 None:intergenic 45.0%
CATTACTTCGTTGGTATCCC+GGG + Chr4:63023236-63023255 MsG0480021694.01.T01:three_prime_UTR 45.0%
GAAAGATTGCAGCTCAATGC+AGG + Chr4:63020185-63020204 MsG0480021694.01.T01:CDS 45.0%
GAAGTGGTAGATGAGGTAAC+AGG + Chr4:63023106-63023125 MsG0480021694.01.T01:CDS 45.0%
GATTCCAGTTATTGTGGGTG+AGG - Chr4:63023355-63023374 None:intergenic 45.0%
GTATCCTCACCCACAATAAC+TGG + Chr4:63023348-63023367 MsG0480021694.01.T01:three_prime_UTR 45.0%
TAAACAATGATTGGGCAGGG+AGG + Chr4:63021469-63021488 MsG0480021694.01.T01:intron 45.0%
TCGTAAGTGCTTATGCCTGT+AGG + Chr4:63020440-63020459 MsG0480021694.01.T01:intron 45.0%
TCTAGATTCCAGAACGGTGA+TGG + Chr4:63022883-63022902 MsG0480021694.01.T01:intron 45.0%
TGCAGTGTTAGTGAGCATAG+AGG - Chr4:63021349-63021368 None:intergenic 45.0%
TTGAACATGATGTCATGGGC+AGG + Chr4:63022988-63023007 MsG0480021694.01.T01:intron 45.0%
! AGAACGGTGATGGCAATTCT+CGG + Chr4:63022893-63022912 MsG0480021694.01.T01:CDS 45.0%
!! GAACAGTTCGTCTTTTAGGG+TGG - Chr4:63021745-63021764 None:intergenic 45.0%
!! GGGTTGATGAGTGTTCATGA+AGG + Chr4:63023188-63023207 MsG0480021694.01.T01:three_prime_UTR 45.0%
!! GGTGCTGGTTATCTTGCTTT+GGG + Chr4:63019861-63019880 MsG0480021694.01.T01:CDS 45.0%
!! TGGTGCTGGTTATCTTGCTT+TGG + Chr4:63019860-63019879 MsG0480021694.01.T01:CDS 45.0%
!!! AGTGCAATTTTGGTTGGTGC+TGG + Chr4:63019846-63019865 MsG0480021694.01.T01:CDS 45.0%
!!! ATTGGGATTTTAGGGTTCCG+GGG - Chr4:63019787-63019806 None:intergenic 45.0%
!!! AAATAAAATTTAAGATAAAA+TGG - Chr4:63022189-63022208 None:intergenic 5.0%
AACAATGATTGGGCAGGGAG+GGG + Chr4:63021471-63021490 MsG0480021694.01.T01:intron 50.0%
AGATCCCTCATTCGCGTGAA+GGG + Chr4:63020606-63020625 MsG0480021694.01.T01:intron 50.0%
ATGTGCAGGACCTGTTGAAG+TGG + Chr4:63023090-63023109 MsG0480021694.01.T01:intron 50.0%
CTGCCGGGATGTAAATCACT+AGG + Chr4:63021931-63021950 MsG0480021694.01.T01:intron 50.0%
GAGAATTGCCATCACCGTTC+TGG - Chr4:63022894-63022913 None:intergenic 50.0%
GATCCCATCTACCACGTCAA+CGG - Chr4:63023015-63023034 None:intergenic 50.0%
GGTTCTTTACCGAGCTCCAA+AGG + Chr4:63020753-63020772 MsG0480021694.01.T01:CDS 50.0%
GTGGTAGATGAGGTAACAGG+TGG + Chr4:63023109-63023128 MsG0480021694.01.T01:CDS 50.0%
TTCACCCTTCACGCGAATGA+GGG - Chr4:63020613-63020632 None:intergenic 50.0%
TTGACGTGGTAGATGGGATC+CGG + Chr4:63023015-63023034 MsG0480021694.01.T01:intron 50.0%
!!! ATTTTAGGGTTCCGGGGAAG+AGG - Chr4:63019781-63019800 None:intergenic 50.0%
AGGACCGTTGACGTGGTAGA+TGG + Chr4:63023008-63023027 MsG0480021694.01.T01:intron 55.0%
CACTCATCAACCCGACCTCA+TGG - Chr4:63023181-63023200 None:intergenic 55.0%
GAGATCCCTCATTCGCGTGA+AGG + Chr4:63020605-63020624 MsG0480021694.01.T01:intron 55.0%
GGACCGTTGACGTGGTAGAT+GGG + Chr4:63023009-63023028 MsG0480021694.01.T01:intron 55.0%
GGAGATGTCTGCACAGCCTA+AGG + Chr4:63020875-63020894 MsG0480021694.01.T01:CDS 55.0%
GTTCACCCTTCACGCGAATG+AGG - Chr4:63020614-63020633 None:intergenic 55.0%
ACGTGGTAGATGGGATCCGG+TGG + Chr4:63023018-63023037 MsG0480021694.01.T01:intron 60.0%
CATGGGCAGGACCGTTGACG+TGG + Chr4:63023001-63023020 MsG0480021694.01.T01:intron 65.0%
GATTGGGCAGGGAGGGGAAG+TGG + Chr4:63021477-63021496 MsG0480021694.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 63019756 63023533 63019756 ID=MsG0480021694.01;Name=MsG0480021694.01
Chr4 mRNA 63019756 63023533 63019756 ID=MsG0480021694.01.T01;Parent=MsG0480021694.01;Name=MsG0480021694.01.T01;_AED=0.50;_eAED=0.51;_QI=72|0.83|0.85|1|0.5|0.42|7|386|200
Chr4 exon 63023098 63023533 63023098 ID=MsG0480021694.01.T01:exon:9381;Parent=MsG0480021694.01.T01
Chr4 exon 63022888 63022914 63022888 ID=MsG0480021694.01.T01:exon:9380;Parent=MsG0480021694.01.T01
Chr4 exon 63022274 63022373 63022274 ID=MsG0480021694.01.T01:exon:9379;Parent=MsG0480021694.01.T01
Chr4 exon 63020872 63020941 63020872 ID=MsG0480021694.01.T01:exon:9378;Parent=MsG0480021694.01.T01
Chr4 exon 63020726 63020785 63020726 ID=MsG0480021694.01.T01:exon:9377;Parent=MsG0480021694.01.T01
Chr4 exon 63020148 63020206 63020148 ID=MsG0480021694.01.T01:exon:9376;Parent=MsG0480021694.01.T01
Chr4 exon 63019756 63020064 63019756 ID=MsG0480021694.01.T01:exon:9375;Parent=MsG0480021694.01.T01
Chr4 five_prime_UTR 63019756 63019827 63019756 ID=MsG0480021694.01.T01:five_prime_utr;Parent=MsG0480021694.01.T01
Chr4 CDS 63019828 63020064 63019828 ID=MsG0480021694.01.T01:cds;Parent=MsG0480021694.01.T01
Chr4 CDS 63020148 63020206 63020148 ID=MsG0480021694.01.T01:cds;Parent=MsG0480021694.01.T01
Chr4 CDS 63020726 63020785 63020726 ID=MsG0480021694.01.T01:cds;Parent=MsG0480021694.01.T01
Chr4 CDS 63020872 63020941 63020872 ID=MsG0480021694.01.T01:cds;Parent=MsG0480021694.01.T01
Chr4 CDS 63022274 63022373 63022274 ID=MsG0480021694.01.T01:cds;Parent=MsG0480021694.01.T01
Chr4 CDS 63022888 63022914 63022888 ID=MsG0480021694.01.T01:cds;Parent=MsG0480021694.01.T01
Chr4 CDS 63023098 63023147 63023098 ID=MsG0480021694.01.T01:cds;Parent=MsG0480021694.01.T01
Chr4 three_prime_UTR 63023148 63023533 63023148 ID=MsG0480021694.01.T01:three_prime_utr;Parent=MsG0480021694.01.T01
Gene Sequence

>MsG0480021694.01.T01

ATGGAAATGTCATGGTTGAGTGCAATTTTGGTTGGTGCTGGTTATCTTGCTTTGGGTTACTTCATTGGTTCTCGTAAAAATTTCATCTTCTCACAGAAATTTTCATTCTCTAAAGACAAAGATGCTTCACTTCTCAATGATGACAAGAAAAATAAGCAGAAGAAGAATACTAAGCCCAAAATCAAAGACCAACTTGAGGTTGAACAGCTTGCTGATATTCTCGAAGATTTTAAGATGATACTTGTTGTTAGAAATGATCTTAAAATGGGTAAAGGAAAGATTGCAGCTCAATGCAGTCATGCAACATTGGGTCTCTATAAAAAGGTTCTTTACCGAGCTCCAAAGGCTTTAAATAGGTGGGAGATGTCTGCACAGCCTAAGGTTGTTGTCAAAATTGAAAGTGAAGAAGATATGCTGGCTTTGCAAGAAAGGGCTAAATCTCTGAAGTTGCCTACTCATATCACAATTGATGCTGGTAGAACACAGATTGCTCCAAGTGAGTATTTGGAACTACCCTTATCTTCTTATTCCAGAACGGTGATGGCAATTCTCGGACCTGTTGAAGTGGTAGATGAGGTAACAGGTGGACTGAAACTTCTATAA
Protein sequence

>MsG0480021694.01.T01

MEMSWLSAILVGAGYLALGYFIGSRKNFIFSQKFSFSKDKDASLLNDDKKNKQKKNTKPKIKDQLEVEQLADILEDFKMILVVRNDLKMGKGKIAAQCSHATLGLYKKVLYRAPKALNRWEMSAQPKVVVKIESEEDMLALQERAKSLKLPTHITIDAGRTQIAPSEYLELPLSSYSRTVMAILGPVEVVDEVTGGLKLL*