Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010495.01.T01 | XP_013464930.1 | 93.805 | 113 | 7 | 0 | 51 | 163 | 1 | 113 | 2.38E-41 | 144 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010495.01.T01 | O24415 | 62.832 | 113 | 42 | 0 | 51 | 163 | 1 | 113 | 2.52E-27 | 101 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010495.01.T01 | A0A072VLF0 | 93.805 | 113 | 7 | 0 | 51 | 163 | 1 | 113 | 1.13e-41 | 144 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048216.01 | MsG0280010495.01 | 0.844630 | 6.711585e-59 | 1.090115e-55 |
| MsG0080048687.01 | MsG0280010495.01 | 0.839433 | 1.589193e-57 | 2.194385e-54 |
| MsG0080048944.01 | MsG0280010495.01 | 0.805358 | 1.387102e-49 | 7.323622e-47 |
| MsG0080048971.01 | MsG0280010495.01 | 0.889120 | 3.218692e-73 | 2.562963e-69 |
| MsG0180000227.01 | MsG0280010495.01 | 0.838356 | 3.018723e-57 | 4.033084e-54 |
| MsG0180000339.01 | MsG0280010495.01 | 0.826351 | 2.845191e-54 | 2.661711e-51 |
| MsG0180000614.01 | MsG0280010495.01 | 0.800673 | 1.290505e-48 | 6.044975e-46 |
| MsG0180000615.01 | MsG0280010495.01 | 0.802784 | 4.759627e-49 | 2.352240e-46 |
| MsG0180003115.01 | MsG0280010495.01 | 0.889114 | 3.236833e-73 | 2.576834e-69 |
| MsG0180003117.01 | MsG0280010495.01 | 0.811348 | 7.321436e-51 | 4.521020e-48 |
| MsG0180003313.01 | MsG0280010495.01 | 0.908409 | 1.765539e-81 | 3.272699e-77 |
| MsG0180003612.01 | MsG0280010495.01 | 0.897332 | 1.558743e-76 | 1.752748e-72 |
| MsG0180003793.01 | MsG0280010495.01 | 0.876275 | 1.589699e-68 | 7.659961e-65 |
| MsG0180004009.01 | MsG0280010495.01 | 0.846661 | 1.887337e-59 | 3.272337e-56 |
| MsG0180004129.01 | MsG0280010495.01 | 0.907021 | 7.946632e-81 | 1.382521e-76 |
| MsG0180004130.01 | MsG0280010495.01 | 0.878461 | 2.756191e-69 | 1.440547e-65 |
| MsG0180004607.01 | MsG0280010495.01 | 0.837137 | 6.206367e-57 | 7.987421e-54 |
| MsG0180004609.01 | MsG0280010495.01 | 0.801198 | 1.008016e-48 | 4.784905e-46 |
| MsG0180004622.01 | MsG0280010495.01 | 0.845115 | 4.967751e-59 | 8.192666e-56 |
| MsG0180004625.01 | MsG0280010495.01 | 0.831753 | 1.396127e-55 | 1.529437e-52 |
| MsG0180004854.01 | MsG0280010495.01 | 0.807891 | 4.049659e-50 | 2.282270e-47 |
| MsG0180005361.01 | MsG0280010495.01 | 0.872239 | 3.703827e-67 | 1.538561e-63 |
| MsG0180005366.01 | MsG0280010495.01 | 0.893126 | 8.398339e-75 | 7.901002e-71 |
| MsG0180005426.01 | MsG0280010495.01 | 0.905960 | 2.468717e-80 | 4.092919e-76 |
| MsG0180005573.01 | MsG0280010495.01 | 0.807609 | 4.649100e-50 | 2.600903e-47 |
| MsG0280006424.01 | MsG0280010495.01 | 0.857665 | 1.398205e-62 | 3.477468e-59 |
| MsG0280006664.01 | MsG0280010495.01 | 0.826987 | 2.005995e-54 | 1.911248e-51 |
| MsG0280006741.01 | MsG0280010495.01 | 0.885864 | 5.626853e-72 | 3.926354e-68 |
| MsG0280006819.01 | MsG0280010495.01 | 0.888269 | 6.860310e-73 | 5.278330e-69 |
| MsG0280006836.01 | MsG0280010495.01 | 0.891444 | 3.951918e-74 | 3.466029e-70 |
| MsG0280006993.01 | MsG0280010495.01 | 0.829271 | 5.650394e-55 | 5.755660e-52 |
| MsG0280007634.01 | MsG0280010495.01 | 0.804617 | 1.982559e-49 | 1.026910e-46 |
| MsG0280007643.01 | MsG0280010495.01 | 0.872848 | 2.320189e-67 | 9.854606e-64 |
| MsG0280008118.01 | MsG0280010495.01 | 0.891973 | 2.434703e-74 | 2.181052e-70 |
| MsG0280008283.01 | MsG0280010495.01 | 0.845490 | 3.931317e-59 | 6.562338e-56 |
| MsG0280009111.01 | MsG0280010495.01 | 0.815945 | 7.126186e-52 | 4.981734e-49 |
| MsG0280009372.01 | MsG0280010495.01 | 0.901906 | 1.667318e-78 | 2.302294e-74 |
| MsG0280009375.01 | MsG0280010495.01 | 0.898586 | 4.589052e-77 | 5.458331e-73 |
| MsG0280010130.01 | MsG0280010495.01 | 0.800246 | 1.576690e-48 | 7.305808e-46 |
| MsG0280010383.01 | MsG0280010495.01 | 0.842501 | 2.488031e-58 | 3.779973e-55 |
| MsG0280010495.01 | MsG0280010525.01 | 0.858618 | 7.278030e-63 | 1.868725e-59 |
| MsG0280010495.01 | MsG0280010801.01 | 0.914110 | 2.814312e-84 | 6.881192e-80 |
| MsG0280010495.01 | MsG0280010995.01 | 0.858962 | 5.744383e-63 | 1.492507e-59 |
| MsG0280010495.01 | MsG0280011060.01 | 0.847171 | 1.368793e-59 | 2.412334e-56 |
| MsG0280010495.01 | MsG0280011234.01 | 0.874836 | 4.944251e-68 | 2.259382e-64 |
| MsG0280010495.01 | MsG0280011308.01 | 0.865806 | 4.519902e-65 | 1.489849e-61 |
| MsG0280010495.01 | MsG0380011643.01 | 0.869687 | 2.569813e-66 | 9.729667e-63 |
| MsG0280010495.01 | MsG0380012073.01 | 0.879745 | 9.699408e-70 | 5.320126e-66 |
| MsG0280010495.01 | MsG0380015494.01 | 0.877739 | 4.932944e-69 | 2.508360e-65 |
| MsG0280010495.01 | MsG0380016577.01 | 0.894615 | 2.087700e-75 | 2.091200e-71 |
| MsG0280010495.01 | MsG0380016622.01 | 0.858608 | 7.328605e-63 | 1.881121e-59 |
| MsG0280010495.01 | MsG0380016678.01 | 0.804700 | 1.904924e-49 | 9.888351e-47 |
| MsG0280010495.01 | MsG0380016733.01 | 0.878597 | 2.468775e-69 | 1.296837e-65 |
| MsG0280010495.01 | MsG0380017382.01 | 0.826862 | 2.148493e-54 | 2.039452e-51 |
| MsG0280010495.01 | MsG0380017556.01 | 0.829713 | 4.413962e-55 | 4.554335e-52 |
| MsG0280010495.01 | MsG0480018084.01 | 0.886775 | 2.550173e-72 | 1.846342e-68 |
| MsG0280010495.01 | MsG0480018252.01 | 0.823334 | 1.464554e-53 | 1.257284e-50 |
| MsG0280010495.01 | MsG0480018477.01 | 0.846213 | 2.501747e-59 | 4.274224e-56 |
| MsG0280010495.01 | MsG0480019632.01 | 0.860423 | 2.086931e-63 | 5.701858e-60 |
| MsG0280010495.01 | MsG0480020895.01 | 0.836956 | 6.905270e-57 | 8.838799e-54 |
| MsG0280010495.01 | MsG0480020959.01 | 0.846705 | 1.836343e-59 | 3.188459e-56 |
| MsG0280010495.01 | MsG0480021006.01 | 0.853781 | 1.904785e-61 | 4.162623e-58 |
| MsG0280010495.01 | MsG0480021159.01 | 0.859978 | 2.845439e-63 | 7.656139e-60 |
| MsG0280010495.01 | MsG0480022363.01 | 0.820146 | 8.002910e-53 | 6.281728e-50 |
| MsG0280010495.01 | MsG0480022693.01 | 0.825264 | 5.153077e-54 | 4.673885e-51 |
| MsG0280010495.01 | MsG0480022764.01 | 0.809883 | 1.518406e-50 | 9.020576e-48 |
| MsG0280010495.01 | MsG0480022789.01 | 0.803453 | 3.461237e-49 | 1.740007e-46 |
| MsG0280010495.01 | MsG0480023149.01 | 0.912304 | 2.270446e-83 | 5.075670e-79 |
| MsG0280010495.01 | MsG0480023399.01 | 0.814354 | 1.608313e-51 | 1.076688e-48 |
| MsG0280010495.01 | MsG0480023467.01 | 0.952899 | 8.928149e-111 | 2.248833e-105 |
| MsG0280010495.01 | MsG0480023559.01 | 0.814081 | 1.846927e-51 | 1.227325e-48 |
| MsG0280010495.01 | MsG0480023970.01 | 0.862408 | 5.176140e-64 | 1.514351e-60 |
| MsG0280010495.01 | MsG0580024369.01 | 0.916713 | 1.278201e-85 | 3.557506e-81 |
| MsG0280010495.01 | MsG0580024753.01 | 0.801816 | 7.530275e-49 | 3.630814e-46 |
| MsG0280010495.01 | MsG0580025073.01 | 0.827789 | 1.288213e-54 | 1.256327e-51 |
| MsG0280010495.01 | MsG0580025348.01 | 0.843820 | 1.107672e-58 | 1.753911e-55 |
| MsG0280010495.01 | MsG0580025750.01 | 0.817873 | 2.631250e-52 | 1.939197e-49 |
| MsG0280010495.01 | MsG0580027043.01 | 0.933770 | 1.129529e-95 | 8.194266e-91 |
| MsG0280010495.01 | MsG0580029132.01 | 0.933270 | 2.422746e-95 | 1.704667e-90 |
| MsG0280010495.01 | MsG0580029702.01 | 0.866833 | 2.135776e-65 | 7.300425e-62 |
| MsG0280010495.01 | MsG0580029703.01 | 0.805832 | 1.103408e-49 | 5.897156e-47 |
| MsG0280010495.01 | MsG0580030073.01 | 0.861492 | 9.878899e-64 | 2.800505e-60 |
| MsG0280010495.01 | MsG0680030968.01 | 0.802629 | 5.124130e-49 | 2.522261e-46 |
| MsG0280010495.01 | MsG0680031763.01 | 0.916643 | 1.391727e-85 | 3.860986e-81 |
| MsG0280010495.01 | MsG0680032770.01 | 0.901795 | 1.865981e-78 | 2.561993e-74 |
| MsG0280010495.01 | MsG0680035848.01 | 0.885294 | 9.207106e-72 | 6.284444e-68 |
| MsG0280010495.01 | MsG0780036240.01 | 0.847528 | 1.092651e-59 | 1.947885e-56 |
| MsG0280010495.01 | MsG0780036547.01 | 0.858576 | 7.491905e-63 | 1.920833e-59 |
| MsG0280010495.01 | MsG0780036822.01 | 0.902529 | 8.829337e-79 | 1.255843e-74 |
| MsG0280010495.01 | MsG0780037097.01 | 0.835958 | 1.238955e-56 | 1.538830e-53 |
| MsG0280010495.01 | MsG0780038383.01 | 0.806952 | 6.407531e-50 | 3.523999e-47 |
| MsG0280010495.01 | MsG0780038400.01 | 0.845623 | 3.617623e-59 | 6.064481e-56 |
| MsG0280010495.01 | MsG0780038587.01 | 0.886651 | 2.840068e-72 | 2.046464e-68 |
| MsG0280010495.01 | MsG0780039271.01 | 0.834880 | 2.320253e-56 | 2.790095e-53 |
| MsG0280010495.01 | MsG0780040338.01 | 0.879334 | 1.356476e-69 | 7.325551e-66 |
| MsG0280010495.01 | MsG0780040782.01 | 0.878202 | 3.399206e-69 | 1.759111e-65 |
| MsG0280010495.01 | MsG0780040900.01 | 0.906267 | 1.779987e-80 | 2.990530e-76 |
| MsG0280010495.01 | MsG0780041058.01 | 0.885627 | 6.904893e-72 | 4.774491e-68 |
| MsG0280010495.01 | MsG0780041147.01 | 0.901191 | 3.439943e-78 | 4.595791e-74 |
| MsG0280010495.01 | MsG0780041223.01 | 0.852431 | 4.638513e-61 | 9.690093e-58 |
| MsG0280010495.01 | MsG0780041332.01 | 0.882415 | 1.062378e-70 | 6.464737e-67 |
| MsG0280010495.01 | MsG0780041461.01 | 0.859594 | 3.711539e-63 | 9.853773e-60 |
| MsG0280010495.01 | MsG0780041519.01 | 0.882166 | 1.307701e-70 | 7.881655e-67 |
| MsG0280010495.01 | MsG0880041967.01 | 0.873301 | 1.634718e-67 | 7.059265e-64 |
| MsG0280010495.01 | MsG0880042125.01 | 0.890335 | 1.080991e-73 | 9.051118e-70 |
| MsG0280010495.01 | MsG0880042209.01 | 0.803054 | 4.186812e-49 | 2.083235e-46 |
| MsG0280010495.01 | MsG0880043517.01 | 0.893609 | 5.361605e-75 | 5.144480e-71 |
| MsG0280010495.01 | MsG0880043770.01 | 0.883678 | 3.661926e-71 | 2.344980e-67 |
| MsG0280010495.01 | MsG0880044003.01 | 0.810544 | 1.093508e-50 | 6.609932e-48 |
| MsG0280010495.01 | MsG0880044711.01 | 0.869666 | 2.608982e-66 | 9.870379e-63 |
| MsG0280010495.01 | MsG0880045072.01 | 0.806256 | 8.983343e-50 | 4.853530e-47 |
| MsG0280010495.01 | MsG0880046177.01 | 0.839145 | 1.888034e-57 | 2.583786e-54 |
| MsG0280010495.01 | MsG0880046444.01 | 0.939156 | 2.040659e-99 | 2.061361e-94 |
| MsG0280010495.01 | MsG0880046575.01 | 0.831186 | 1.925015e-55 | 2.073407e-52 |
| MsG0280010495.01 | MsG0880046879.01 | 0.919605 | 3.652487e-87 | 1.182748e-82 |
| MsG0280010495.01 | MsG0880047082.01 | 0.862270 | 5.709194e-64 | 1.662070e-60 |
| MsG0280010495.01 | MsG0880047388.01 | 0.825883 | 3.677256e-54 | 3.394679e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0780041483.01 | MsG0280010495.01 | PPI |
| MsG0780036073.01 | MsG0280010495.01 | PPI |
| MsG0380016607.01 | MsG0280010495.01 | PPI |
| MsG0280010495.01 | MsG0580028409.01 | PPI |
| MsG0480021694.01 | MsG0280010495.01 | PPI |
| MsG0280010495.01 | MsG0380016653.01 | PPI |
| MsG0580024324.01 | MsG0280010495.01 | PPI |
| MsG0380016607.01 | MsG0280010495.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010495.01.T01 | MTR_2g085085 | 93.805 | 113 | 7 | 0 | 51 | 163 | 1 | 113 | 2.88e-45 | 144 |
| MsG0280010495.01.T01 | MTR_0227s0020 | 84.956 | 113 | 17 | 0 | 51 | 163 | 1 | 113 | 3.16e-37 | 124 |
| MsG0280010495.01.T01 | MTR_1g060600 | 69.027 | 113 | 34 | 1 | 51 | 163 | 1 | 112 | 2.54e-29 | 104 |
| MsG0280010495.01.T01 | MTR_2g085105 | 96.552 | 29 | 1 | 0 | 51 | 79 | 1 | 29 | 2.75e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010495.01.T01 | AT2G27710 | 76.119 | 67 | 16 | 0 | 51 | 117 | 1 | 67 | 4.54e-29 | 103 |
| MsG0280010495.01.T01 | AT2G27720 | 60.870 | 115 | 43 | 1 | 51 | 163 | 1 | 115 | 3.71e-27 | 99.0 |
| MsG0280010495.01.T01 | AT3G44590 | 64.602 | 113 | 38 | 2 | 51 | 163 | 1 | 111 | 1.14e-26 | 97.4 |
| MsG0280010495.01.T01 | AT3G44590 | 64.602 | 113 | 38 | 2 | 51 | 163 | 1 | 111 | 1.14e-26 | 97.4 |
| MsG0280010495.01.T01 | AT2G27710 | 74.194 | 62 | 16 | 0 | 51 | 112 | 1 | 62 | 2.41e-25 | 94.4 |
| MsG0280010495.01.T01 | AT2G27710 | 74.194 | 62 | 16 | 0 | 51 | 112 | 1 | 62 | 2.41e-25 | 94.4 |
| MsG0280010495.01.T01 | AT2G27710 | 74.194 | 62 | 16 | 0 | 51 | 112 | 1 | 62 | 2.41e-25 | 94.4 |
| MsG0280010495.01.T01 | AT2G27720 | 55.118 | 127 | 43 | 2 | 51 | 163 | 1 | 127 | 1.42e-23 | 90.1 |
| MsG0280010495.01.T01 | AT2G27720 | 53.077 | 130 | 44 | 2 | 51 | 163 | 1 | 130 | 3.84e-22 | 86.7 |
| MsG0280010495.01.T01 | AT3G28500 | 53.913 | 115 | 51 | 2 | 51 | 163 | 1 | 115 | 3.84e-22 | 85.9 |
| MsG0280010495.01.T01 | AT5G40040 | 55.263 | 114 | 50 | 1 | 51 | 163 | 1 | 114 | 1.20e-15 | 69.3 |
| MsG0280010495.01.T01 | AT2G27720 | 59.770 | 87 | 33 | 1 | 79 | 163 | 5 | 91 | 1.41e-12 | 60.5 |
Find 45 sgRNAs with CRISPR-Local
Find 130 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTACTTGTTGGCCGTTT+TGG | 0.128749 | 2:-70550116 | MsG0280010495.01.T01:CDS |
| ATTCAGCTTATTTGATTAAA+AGG | 0.186887 | 2:-70548672 | MsG0280010495.01.T01:exon |
| TAACAATGTGACAAGTAATT+TGG | 0.231724 | 2:+70550966 | None:intergenic |
| GCTGATCTTAAACAAATTCT+TGG | 0.267351 | 2:-70550070 | MsG0280010495.01.T01:CDS |
| GTTGCTGTGTCTGCTGCAAC+TGG | 0.362343 | 2:-70548869 | MsG0280010495.01.T01:CDS |
| TCTTCCTTTGCTGCTTCAGC+AGG | 0.364467 | 2:+70548824 | None:intergenic |
| CTTAAACAAATTCTTGGATC+AGG | 0.372982 | 2:-70550064 | MsG0280010495.01.T01:intron |
| TTTCAATTTAATCTGTGTTA+TGG | 0.373884 | 2:-70548534 | MsG0280010495.01.T01:three_prime_UTR |
| GTTATCTTTGTGCAGGATAT+GGG | 0.399106 | 2:-70548695 | MsG0280010495.01.T01:intron |
| AGTGAATATTAACAGATGTT+AGG | 0.430898 | 2:+70550999 | None:intergenic |
| AGAGAAGAAGGTGGAAGAGA+AGG | 0.434796 | 2:-70548804 | MsG0280010495.01.T01:CDS |
| TTACTTGTCACATTGTTAAG+TGG | 0.441335 | 2:-70550961 | MsG0280010495.01.T01:CDS |
| GATTATCGCAGCTTACTTGT+TGG | 0.445399 | 2:-70550125 | MsG0280010495.01.T01:CDS |
| GTGTCTGCTGCAACTGGAGG+AGG | 0.445905 | 2:-70548863 | MsG0280010495.01.T01:CDS |
| TGCGCCTGCTGAAGCAGCAA+AGG | 0.454235 | 2:-70548828 | MsG0280010495.01.T01:CDS |
| GATCAGCAGCTGAAGGGGAT+TGG | 0.469713 | 2:+70550086 | None:intergenic |
| CCTTGCCCTTGACTTCACTT+AGG | 0.488662 | 2:+70548957 | None:intergenic |
| TGCCAGTGGAAGAGAAAAGT+TGG | 0.491656 | 2:-70548918 | MsG0280010495.01.T01:CDS |
| GCAACAGCACACCACACGGA+TGG | 0.492797 | 2:+70548887 | None:intergenic |
| AGCAGCAAAGGAAGAGAAGA+AGG | 0.518126 | 2:-70548816 | MsG0280010495.01.T01:CDS |
| TAACACATCGATCTTGATGA+AGG | 0.521468 | 2:+70550936 | None:intergenic |
| CACAGCAACAGCACACCACA+CGG | 0.524318 | 2:+70548883 | None:intergenic |
| GGATTGGTTGCCTCCCAAAA+CGG | 0.534645 | 2:+70550102 | None:intergenic |
| TGTAGTGACATTGGTGTTGG+CGG | 0.535179 | 2:-70550884 | MsG0280010495.01.T01:intron |
| CAACTCAATGTTGCCATCCT+CGG | 0.536919 | 2:+70548982 | None:intergenic |
| TGTTTAAGATCAGCAGCTGA+AGG | 0.541739 | 2:+70550079 | None:intergenic |
| TGTTGTGATTGTAGTGACAT+TGG | 0.544553 | 2:-70550893 | MsG0280010495.01.T01:CDS |
| TCTTCCACTGGCAATAAGCT+CGG | 0.547245 | 2:+70548928 | None:intergenic |
| GATTGTAGTGACATTGGTGT+TGG | 0.553130 | 2:-70550887 | MsG0280010495.01.T01:CDS |
| GTTTAAGATCAGCAGCTGAA+GGG | 0.564265 | 2:+70550080 | None:intergenic |
| TTTAAGATCAGCAGCTGAAG+GGG | 0.564880 | 2:+70550081 | None:intergenic |
| GTTGGAGTTGAGGCCGAGGA+TGG | 0.573673 | 2:-70548995 | MsG0280010495.01.T01:CDS |
| GTTGCTCCTAAGTGAAGTCA+AGG | 0.590863 | 2:-70548963 | MsG0280010495.01.T01:CDS |
| GCTGTGTCTGCTGCAACTGG+AGG | 0.592795 | 2:-70548866 | MsG0280010495.01.T01:CDS |
| TATGATTGTAGTTGGAGTTG+AGG | 0.599920 | 2:-70549005 | MsG0280010495.01.T01:intron |
| TTTGCCGAGCTTATTGCCAG+TGG | 0.606365 | 2:-70548932 | MsG0280010495.01.T01:CDS |
| TCATGAATCACCACTAGAGG+AGG | 0.607661 | 2:+70548453 | None:intergenic |
| AATTCATGAATCACCACTAG+AGG | 0.612550 | 2:+70548450 | None:intergenic |
| CCTAAGTGAAGTCAAGGGCA+AGG | 0.616488 | 2:-70548957 | MsG0280010495.01.T01:CDS |
| TGTAGTTGGAGTTGAGGCCG+AGG | 0.617745 | 2:-70548999 | MsG0280010495.01.T01:intron |
| AACACATCGATCTTGATGAA+GGG | 0.617849 | 2:+70550937 | None:intergenic |
| TGGCTTCAGTTCCATCCGTG+TGG | 0.667907 | 2:-70548898 | MsG0280010495.01.T01:CDS |
| TTGCTCCTAAGTGAAGTCAA+GGG | 0.680870 | 2:-70548962 | MsG0280010495.01.T01:CDS |
| AGCAAAGGAAGAGAAGAAGG+TGG | 0.683753 | 2:-70548813 | MsG0280010495.01.T01:CDS |
| ACAAGTAATTTGGTGCACAG+AGG | 0.723389 | 2:+70550976 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAGATTAAAGATTAATT+GGG | + | Chr2:70549383-70549402 | None:intergenic | 10.0% |
| !!! | AAATAATTTTAATTCAAGTT+TGG | - | Chr2:70549784-70549803 | MsG0280010495.01.T01:intron | 10.0% |
| !!! | AATAATTTTAATTCAAGTTT+GGG | - | Chr2:70549785-70549804 | MsG0280010495.01.T01:intron | 10.0% |
| !! | AACAAAATAAAAATAGAACA+AGG | - | Chr2:70549014-70549033 | MsG0280010495.01.T01:intron | 15.0% |
| !! | AATGAAAATTGATCAAATAT+TGG | - | Chr2:70549865-70549884 | MsG0280010495.01.T01:intron | 15.0% |
| !! | ACAAAATAAAAATAGAACAA+GGG | - | Chr2:70549015-70549034 | MsG0280010495.01.T01:intron | 15.0% |
| !! | ACAATAACTATATTCTTTAT+TGG | - | Chr2:70548726-70548745 | MsG0280010495.01.T01:intron | 15.0% |
| !! | ATTAAAATCAAACCATATAT+GGG | + | Chr2:70550136-70550155 | None:intergenic | 15.0% |
| !! | GAATAAGATTAAAGATTAAT+TGG | + | Chr2:70549384-70549403 | None:intergenic | 15.0% |
| !!! | AAAAAAAGTGACTTTATTTT+TGG | - | Chr2:70550380-70550399 | MsG0280010495.01.T01:intron | 15.0% |
| !!! | AAAAAAGTGACTTTATTTTT+GGG | - | Chr2:70550381-70550400 | MsG0280010495.01.T01:intron | 15.0% |
| !!! | TTGTTCTATTTTTATTTTGT+TGG | + | Chr2:70549015-70549034 | None:intergenic | 15.0% |
| !!! | TTTAACTTTAATTCAAGTTT+GGG | - | Chr2:70549818-70549837 | MsG0280010495.01.T01:intron | 15.0% |
| !! | AATAGTTTATGCAAATTCAT+AGG | - | Chr2:70550050-70550069 | MsG0280010495.01.T01:intron | 20.0% |
| !! | ATGGATCTACTAAAATAATT+CGG | + | Chr2:70549477-70549496 | None:intergenic | 20.0% |
| !! | ATTCAGCTTATTTGATTAAA+AGG | - | Chr2:70550739-70550758 | MsG0280010495.01.T01:intron | 20.0% |
| !! | CATTAAAATCAAACCATATA+TGG | + | Chr2:70550137-70550156 | None:intergenic | 20.0% |
| !! | TTAAAATCAAACCATATATG+GGG | + | Chr2:70550135-70550154 | None:intergenic | 20.0% |
| !! | TTTCAATTTAATCTGTGTTA+TGG | - | Chr2:70550877-70550896 | MsG0280010495.01.T01:intron | 20.0% |
| !!! | AAATATTGGTTTATTTGGAT+TGG | - | Chr2:70549879-70549898 | MsG0280010495.01.T01:intron | 20.0% |
| !!! | AATTTTGAAACTTACATCAT+CGG | + | Chr2:70550639-70550658 | None:intergenic | 20.0% |
| !!! | AGGATTTGTTAATTGATAAT+CGG | - | Chr2:70549643-70549662 | MsG0280010495.01.T01:intron | 20.0% |
| !!! | ATATTCTACACTTTTACTTA+TGG | + | Chr2:70549496-70549515 | None:intergenic | 20.0% |
| !!! | CACGAAATTGATTTTTTTTT+TGG | + | Chr2:70548944-70548963 | None:intergenic | 20.0% |
| !!! | GTTTAACTTTAATTCAAGTT+TGG | - | Chr2:70549817-70549836 | MsG0280010495.01.T01:intron | 20.0% |
| !!! | TGATCAAATATTGGTTTATT+TGG | - | Chr2:70549874-70549893 | MsG0280010495.01.T01:intron | 20.0% |
| !!! | TGCTAATTTAATTCAAGTTT+AGG | - | Chr2:70549623-70549642 | MsG0280010495.01.T01:intron | 20.0% |
| ! | AGTGAATATTAACAGATGTT+AGG | + | Chr2:70548415-70548434 | None:intergenic | 25.0% |
| ! | TAAAATCGATGAATGAACAT+GGG | - | Chr2:70549672-70549691 | MsG0280010495.01.T01:intron | 25.0% |
| ! | TAACAATGTGACAAGTAATT+TGG | + | Chr2:70548448-70548467 | None:intergenic | 25.0% |
| ! | TGTTTATCATGATTTGATGT+TGG | - | Chr2:70548636-70548655 | MsG0280010495.01.T01:three_prime_UTR | 25.0% |
| ! | TTAAAATCGATGAATGAACA+TGG | - | Chr2:70549671-70549690 | MsG0280010495.01.T01:intron | 25.0% |
| ! | TTGGTGAAATTTATGTTTGA+AGG | - | Chr2:70548745-70548764 | MsG0280010495.01.T01:intron | 25.0% |
| !! | TAGTAGTATTGTTTGTTACT+TGG | - | Chr2:70550661-70550680 | MsG0280010495.01.T01:intron | 25.0% |
| !! | TAGTTATTGTTGTTGAAAGA+TGG | + | Chr2:70548716-70548735 | None:intergenic | 25.0% |
| !! | TTTTACTGTTGAAGGAAAAA+TGG | - | Chr2:70550277-70550296 | MsG0280010495.01.T01:intron | 25.0% |
| !!! | ATTTTAATTCAAGTTTGGGT+TGG | - | Chr2:70549789-70549808 | MsG0280010495.01.T01:intron | 25.0% |
| !!! | GCATTTTTTGTTTGTTTTTC+CGG | - | Chr2:70549435-70549454 | MsG0280010495.01.T01:intron | 25.0% |
| !!! | GCTTAGTTTTATACATTGTT+TGG | - | Chr2:70550156-70550175 | MsG0280010495.01.T01:intron | 25.0% |
| !!! | GTATCTTTTTTTACTGTTGA+AGG | - | Chr2:70550269-70550288 | MsG0280010495.01.T01:intron | 25.0% |
| !!! | TTTTAATTCAAGTTTGGGTT+GGG | - | Chr2:70549790-70549809 | MsG0280010495.01.T01:intron | 25.0% |
| !!! | TTTTTTTGGAGAAAAGACAA+AGG | + | Chr2:70548930-70548949 | None:intergenic | 25.0% |
| AAATAATCAATCCTAGACTG+TGG | - | Chr2:70549578-70549597 | MsG0280010495.01.T01:intron | 30.0% | |
| AAGAAGAAGAGTAAGAACTA+GGG | + | Chr2:70549069-70549088 | None:intergenic | 30.0% | |
| AATAATCAATCCTAGACTGT+GGG | - | Chr2:70549579-70549598 | MsG0280010495.01.T01:intron | 30.0% | |
| CATCAACAACTATATTCAAC+AGG | - | Chr2:70548660-70548679 | MsG0280010495.01.T01:exon | 30.0% | |
| CTTAAACAAATTCTTGGATC+AGG | - | Chr2:70549347-70549366 | MsG0280010495.01.T01:intron | 30.0% | |
| GAAATCGATATCTTACTTGA+TGG | + | Chr2:70549178-70549197 | None:intergenic | 30.0% | |
| GATATCTTACTTGATGGAAA+TGG | + | Chr2:70549172-70549191 | None:intergenic | 30.0% | |
| GCTGATCTTAAACAAATTCT+TGG | - | Chr2:70549341-70549360 | MsG0280010495.01.T01:intron | 30.0% | |
| TAAGAAGAAGAGTAAGAACT+AGG | + | Chr2:70549070-70549089 | None:intergenic | 30.0% | |
| TATCTACTGTTAGACTGTTA+AGG | - | Chr2:70549915-70549934 | MsG0280010495.01.T01:intron | 30.0% | |
| TTACTTGTCACATTGTTAAG+TGG | - | Chr2:70548450-70548469 | MsG0280010495.01.T01:three_prime_UTR | 30.0% | |
| ! | AAACTTCACTCTTTTACGTT+TGG | - | Chr2:70549206-70549225 | MsG0280010495.01.T01:intron | 30.0% |
| ! | AACTTCACTCTTTTACGTTT+GGG | - | Chr2:70549207-70549226 | MsG0280010495.01.T01:intron | 30.0% |
| ! | TTATTGTTGTTGAAAGATGG+AGG | + | Chr2:70548713-70548732 | None:intergenic | 30.0% |
| !! | CGTGTTTAATTTGAAAACTG+AGG | - | Chr2:70548960-70548979 | MsG0280010495.01.T01:CDS | 30.0% |
| AACACATCGATCTTGATGAA+GGG | + | Chr2:70548477-70548496 | None:intergenic | 35.0% | |
| AATTCATGAATCACCACTAG+AGG | + | Chr2:70550964-70550983 | None:intergenic | 35.0% | |
| ATCTTACTTGATGGAAATGG+CGG | + | Chr2:70549169-70549188 | None:intergenic | 35.0% | |
| GTTATCTTTGTGCAGGATAT+GGG | - | Chr2:70550716-70550735 | MsG0280010495.01.T01:intron | 35.0% | |
| TAACACATCGATCTTGATGA+AGG | + | Chr2:70548478-70548497 | None:intergenic | 35.0% | |
| TAATCAAACAGCCCCATATA+TGG | - | Chr2:70550121-70550140 | MsG0280010495.01.T01:CDS | 35.0% | |
| TATGATTGTAGTTGGAGTTG+AGG | - | Chr2:70550406-70550425 | MsG0280010495.01.T01:intron | 35.0% | |
| TATTCAACAGGTTATTTGCG+AGG | - | Chr2:70548672-70548691 | MsG0280010495.01.T01:exon | 35.0% | |
| TCCGAACTTGGTTAAGAAAT+CGG | + | Chr2:70548824-70548843 | None:intergenic | 35.0% | |
| TGTTATCTTTGTGCAGGATA+TGG | - | Chr2:70550715-70550734 | MsG0280010495.01.T01:intron | 35.0% | |
| TGTTGTGATTGTAGTGACAT+TGG | - | Chr2:70548518-70548537 | MsG0280010495.01.T01:three_prime_UTR | 35.0% | |
| TTATCTACTGTTGGACAGTT+GGG | - | Chr2:70549721-70549740 | MsG0280010495.01.T01:intron | 35.0% | |
| TTTGGGTCTATGATTGTAGT+TGG | - | Chr2:70550398-70550417 | MsG0280010495.01.T01:intron | 35.0% | |
| ! | AAGAGTAAGAACTAGGGTTT+TGG | + | Chr2:70549063-70549082 | None:intergenic | 35.0% |
| CGGATACTAATCATCGAAAC+CGG | + | Chr2:70549457-70549476 | None:intergenic | 40.0% | |
| CGTTTGAGCTTATCTACTGT+TGG | - | Chr2:70549712-70549731 | MsG0280010495.01.T01:intron | 40.0% | |
| CTAGCTTATCTTCTTACGAG+AGG | - | Chr2:70548582-70548601 | MsG0280010495.01.T01:three_prime_UTR | 40.0% | |
| CTTATCTACTGTTGGACAGT+TGG | - | Chr2:70549720-70549739 | MsG0280010495.01.T01:intron | 40.0% | |
| CTTCATTATACCCACAGTCT+AGG | + | Chr2:70549592-70549611 | None:intergenic | 40.0% | |
| GATTATCGCAGCTTACTTGT+TGG | - | Chr2:70549286-70549305 | MsG0280010495.01.T01:intron | 40.0% | |
| GATTGTAGTGACATTGGTGT+TGG | - | Chr2:70548524-70548543 | MsG0280010495.01.T01:three_prime_UTR | 40.0% | |
| GCGTAATGTTATCTTTGTGC+AGG | - | Chr2:70550709-70550728 | MsG0280010495.01.T01:intron | 40.0% | |
| TGAATGAACATGGGTCTGTT+TGG | - | Chr2:70549681-70549700 | MsG0280010495.01.T01:intron | 40.0% | |
| TTGCTCCTAAGTGAAGTCAA+GGG | - | Chr2:70550449-70550468 | MsG0280010495.01.T01:intron | 40.0% | |
| ! | GCCGATTTCTTAACCAAGTT+CGG | - | Chr2:70548820-70548839 | MsG0280010495.01.T01:CDS | 40.0% |
| ! | GTTTAAGATCAGCAGCTGAA+GGG | + | Chr2:70549334-70549353 | None:intergenic | 40.0% |
| ! | TGTTTAAGATCAGCAGCTGA+AGG | + | Chr2:70549335-70549354 | None:intergenic | 40.0% |
| ! | TTTAAGATCAGCAGCTGAAG+GGG | + | Chr2:70549333-70549352 | None:intergenic | 40.0% |
| !! | ACAAGTAATTTGGTGCACAG+AGG | + | Chr2:70548438-70548457 | None:intergenic | 40.0% |
| !! | CATTTTGAGAGACTCACTGT+CGG | - | Chr2:70548866-70548885 | MsG0280010495.01.T01:CDS | 40.0% |
| !! | CCAAGTTCGGACACTTTTTA+TGG | - | Chr2:70548833-70548852 | MsG0280010495.01.T01:CDS | 40.0% |
| !! | CCATAAAAAGTGTCCGAACT+TGG | + | Chr2:70548836-70548855 | None:intergenic | 40.0% |
| AGAGAAGAAGGTGGAAGAGA+AGG | - | Chr2:70550607-70550626 | MsG0280010495.01.T01:intron | 45.0% | |
| AGCAAAGGAAGAGAAGAAGG+TGG | - | Chr2:70550598-70550617 | MsG0280010495.01.T01:intron | 45.0% | |
| AGCAGCAAAGGAAGAGAAGA+AGG | - | Chr2:70550595-70550614 | MsG0280010495.01.T01:intron | 45.0% | |
| CAACTCAATGTTGCCATCCT+CGG | + | Chr2:70550432-70550451 | None:intergenic | 45.0% | |
| CTTATCTTCTTACGAGAGGC+GGG | - | Chr2:70548586-70548605 | MsG0280010495.01.T01:three_prime_UTR | 45.0% | |
| GAACATGGGTCTGTTTGGAT+TGG | - | Chr2:70549686-70549705 | MsG0280010495.01.T01:intron | 45.0% | |
| GCTTATCTTCTTACGAGAGG+CGG | - | Chr2:70548585-70548604 | MsG0280010495.01.T01:three_prime_UTR | 45.0% | |
| GTTGCTCCTAAGTGAAGTCA+AGG | - | Chr2:70550448-70550467 | MsG0280010495.01.T01:intron | 45.0% | |
| TCATGAATCACCACTAGAGG+AGG | + | Chr2:70550961-70550980 | None:intergenic | 45.0% | |
| TCTTCCACTGGCAATAAGCT+CGG | + | Chr2:70550486-70550505 | None:intergenic | 45.0% | |
| TGCCAGTGGAAGAGAAAAGT+TGG | - | Chr2:70550493-70550512 | MsG0280010495.01.T01:intron | 45.0% | |
| TTATCTTCTTACGAGAGGCG+GGG | - | Chr2:70548587-70548606 | MsG0280010495.01.T01:three_prime_UTR | 45.0% | |
| ! | AGCCAACTTTTCTCTTCCAC+TGG | + | Chr2:70550498-70550517 | None:intergenic | 45.0% |
| ! | TCTCTCTTTTCCTCCTCTAG+TGG | - | Chr2:70550948-70550967 | MsG0280010495.01.T01:CDS | 45.0% |
| ! | TGCTCGTGATGCATTTTCTG+CGG | - | Chr2:70549095-70549114 | MsG0280010495.01.T01:intron | 45.0% |
| !! | AGCTTACTTGTTGGCCGTTT+TGG | - | Chr2:70549295-70549314 | MsG0280010495.01.T01:intron | 45.0% |
| !! | GCTTACTTGTTGGCCGTTTT+GGG | - | Chr2:70549296-70549315 | MsG0280010495.01.T01:intron | 45.0% |
| !! | TGTAGTGACATTGGTGTTGG+CGG | - | Chr2:70548527-70548546 | MsG0280010495.01.T01:three_prime_UTR | 45.0% |
| CCTAAGTGAAGTCAAGGGCA+AGG | - | Chr2:70550454-70550473 | MsG0280010495.01.T01:intron | 50.0% | |
| CCTTGCCCTTGACTTCACTT+AGG | + | Chr2:70550457-70550476 | None:intergenic | 50.0% | |
| TATCTTCTTACGAGAGGCGG+GGG | - | Chr2:70548588-70548607 | MsG0280010495.01.T01:three_prime_UTR | 50.0% | |
| TTTGCCGAGCTTATTGCCAG+TGG | - | Chr2:70550479-70550498 | MsG0280010495.01.T01:intron | 50.0% | |
| ! | CGGATGAGATCTCGACTTGA+CGG | + | Chr2:70549149-70549168 | None:intergenic | 50.0% |
| ! | GAGATCTCGACTTGACGGTA+GGG | + | Chr2:70549144-70549163 | None:intergenic | 50.0% |
| ! | TCTTCCTTTGCTGCTTCAGC+AGG | + | Chr2:70550590-70550609 | None:intergenic | 50.0% |
| ! | TGAGATCTCGACTTGACGGT+AGG | + | Chr2:70549145-70549164 | None:intergenic | 50.0% |
| !! | GGATTGGTTGCCTCCCAAAA+CGG | + | Chr2:70549312-70549331 | None:intergenic | 50.0% |
| !! | TACTTGTTGGCCGTTTTGGG+AGG | - | Chr2:70549299-70549318 | MsG0280010495.01.T01:intron | 50.0% |
| CACAGCAACAGCACACCACA+CGG | + | Chr2:70550531-70550550 | None:intergenic | 55.0% | |
| GACGGTAGGGTTTCACCAGA+GGG | + | Chr2:70549131-70549150 | None:intergenic | 55.0% | |
| GATCAGCAGCTGAAGGGGAT+TGG | + | Chr2:70549328-70549347 | None:intergenic | 55.0% | |
| GTTGCTGTGTCTGCTGCAAC+TGG | - | Chr2:70550542-70550561 | MsG0280010495.01.T01:intron | 55.0% | |
| TGACGGTAGGGTTTCACCAG+AGG | + | Chr2:70549132-70549151 | None:intergenic | 55.0% | |
| TGGCTTCAGTTCCATCCGTG+TGG | - | Chr2:70550513-70550532 | MsG0280010495.01.T01:intron | 55.0% | |
| TGTAGTTGGAGTTGAGGCCG+AGG | - | Chr2:70550412-70550431 | MsG0280010495.01.T01:intron | 55.0% | |
| GCAACAGCACACCACACGGA+TGG | + | Chr2:70550527-70550546 | None:intergenic | 60.0% | |
| GCTGTGTCTGCTGCAACTGG+AGG | - | Chr2:70550545-70550564 | MsG0280010495.01.T01:intron | 60.0% | |
| GTGTCTGCTGCAACTGGAGG+AGG | - | Chr2:70550548-70550567 | MsG0280010495.01.T01:intron | 60.0% | |
| GTTGGAGTTGAGGCCGAGGA+TGG | - | Chr2:70550416-70550435 | MsG0280010495.01.T01:intron | 60.0% | |
| TGCGCCTGCTGAAGCAGCAA+AGG | - | Chr2:70550583-70550602 | MsG0280010495.01.T01:intron | 60.0% | |
| TGCGGCTTGCAACATCCCTC+TGG | - | Chr2:70549113-70549132 | MsG0280010495.01.T01:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 70548401 | 70551032 | 70548401 | ID=MsG0280010495.01;Name=MsG0280010495.01 |
| Chr2 | mRNA | 70548401 | 70551032 | 70548401 | ID=MsG0280010495.01.T01;Parent=MsG0280010495.01;Name=MsG0280010495.01.T01;_AED=0.41;_eAED=0.41;_QI=0|0.66|0.75|1|1|1|4|275|163 |
| Chr2 | exon | 70548401 | 70548702 | 70548401 | ID=MsG0280010495.01.T01:exon:10051;Parent=MsG0280010495.01.T01 |
| Chr2 | exon | 70548790 | 70549016 | 70548790 | ID=MsG0280010495.01.T01:exon:10050;Parent=MsG0280010495.01.T01 |
| Chr2 | exon | 70550065 | 70550154 | 70550065 | ID=MsG0280010495.01.T01:exon:10049;Parent=MsG0280010495.01.T01 |
| Chr2 | exon | 70550885 | 70551032 | 70550885 | ID=MsG0280010495.01.T01:exon:10048;Parent=MsG0280010495.01.T01 |
| Chr2 | CDS | 70550885 | 70551032 | 70550885 | ID=MsG0280010495.01.T01:cds;Parent=MsG0280010495.01.T01 |
| Chr2 | CDS | 70550065 | 70550154 | 70550065 | ID=MsG0280010495.01.T01:cds;Parent=MsG0280010495.01.T01 |
| Chr2 | CDS | 70548790 | 70549016 | 70548790 | ID=MsG0280010495.01.T01:cds;Parent=MsG0280010495.01.T01 |
| Chr2 | CDS | 70548676 | 70548702 | 70548676 | ID=MsG0280010495.01.T01:cds;Parent=MsG0280010495.01.T01 |
| Chr2 | three_prime_UTR | 70548401 | 70548675 | 70548401 | ID=MsG0280010495.01.T01:three_prime_utr;Parent=MsG0280010495.01.T01 |
| Gene Sequence |
| Protein sequence |
