AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021792.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021792.01.T01 MTR_4g076490 91.623 191 16 0 68 258 231 421 1.71e-127 368
MsG0480021792.01.T01 MTR_4g076470 70.388 206 60 1 34 239 123 327 4.40e-100 295
MsG0480021792.01.T01 MTR_4g076440 81.395 172 31 1 68 239 157 327 4.69e-100 295
MsG0480021792.01.T01 MTR_4g076430 80.814 172 32 1 68 239 157 327 1.86e-97 288
MsG0480021792.01.T01 MTR_4g076255 72.093 172 48 0 68 239 158 329 5.54e-90 270
MsG0480021792.01.T01 MTR_4g076500 72.674 172 44 2 68 236 214 385 9.51e-82 250
MsG0480021792.01.T01 MTR_4g076570 54.211 190 83 1 62 247 152 341 4.14e-66 209
MsG0480021792.01.T01 MTR_1g052430 55.914 186 72 2 62 240 162 344 3.32e-65 208
MsG0480021792.01.T01 MTR_2g034480 56.069 173 70 3 68 236 165 335 5.17e-57 186
MsG0480021792.01.T01 MTR_2g034470 48.259 201 97 4 62 258 127 324 3.04e-55 181
MsG0480021792.01.T01 MTR_2g034470 48.259 201 97 4 62 258 162 359 6.58e-55 181
MsG0480021792.01.T01 MTR_2g034440 51.111 180 81 4 62 236 154 331 1.97e-53 176
MsG0480021792.01.T01 MTR_7g081350 46.857 175 86 4 68 236 160 333 1.37e-49 166
MsG0480021792.01.T01 MTR_5g085580 45.604 182 88 4 68 238 161 342 1.55e-45 159
MsG0480021792.01.T01 MTR_8g102340 38.164 207 111 5 59 252 125 327 2.25e-45 159
MsG0480021792.01.T01 MTR_3g080410 44.615 195 97 5 68 251 162 356 2.39e-45 156
MsG0480021792.01.T01 MTR_2g086530 41.667 180 95 3 70 239 144 323 3.47e-44 153
MsG0480021792.01.T01 MTR_2g086530 41.899 179 94 3 70 238 165 343 4.21e-44 153
MsG0480021792.01.T01 MTR_6g088885 38.660 194 105 4 59 242 158 347 1.35e-42 152
MsG0480021792.01.T01 MTR_1g056640 42.347 196 100 5 70 254 176 369 1.30e-41 149
MsG0480021792.01.T01 MTR_4g010200 38.503 187 105 3 68 244 170 356 1.88e-41 146
MsG0480021792.01.T01 MTR_4g084480 43.407 182 93 5 71 242 164 345 4.11e-41 145
MsG0480021792.01.T01 MTR_4g084480 43.407 182 93 5 71 242 171 352 4.34e-41 145
MsG0480021792.01.T01 MTR_4g084480 43.407 182 93 5 71 242 171 352 4.38e-41 145
MsG0480021792.01.T01 MTR_7g081370 43.023 172 84 4 68 236 161 321 1.17e-40 142
MsG0480021792.01.T01 MTR_4g088155 40.314 191 101 4 70 247 164 354 1.62e-40 145
MsG0480021792.01.T01 MTR_4g052970 42.077 183 96 4 70 242 167 349 9.87e-40 142
MsG0480021792.01.T01 MTR_1g077990 43.646 181 93 5 70 241 169 349 2.66e-39 141
MsG0480021792.01.T01 MTR_5g078200 39.604 202 105 5 70 254 128 329 3.11e-39 144
MsG0480021792.01.T01 MTR_5g078200 39.604 202 105 5 70 254 161 362 4.03e-39 144
MsG0480021792.01.T01 MTR_7g053500 40.107 187 96 5 71 242 88 273 5.61e-39 140
MsG0480021792.01.T01 MTR_8g012400 38.710 186 100 4 71 242 88 273 1.42e-38 139
MsG0480021792.01.T01 MTR_7g053500 40.107 187 96 5 71 242 165 350 1.56e-38 140
MsG0480021792.01.T01 MTR_1g031900 39.247 186 100 3 70 242 162 347 2.77e-38 140
MsG0480021792.01.T01 MTR_8g012400 38.710 186 100 4 71 242 168 353 4.11e-38 139
MsG0480021792.01.T01 MTR_1g031900 39.247 186 100 3 70 242 162 347 7.74e-38 139
MsG0480021792.01.T01 MTR_1g007810 40.884 181 90 6 68 236 153 328 2.10e-37 135
MsG0480021792.01.T01 MTR_1g022160 38.798 183 101 4 70 241 163 345 5.82e-37 135
MsG0480021792.01.T01 MTR_1g022160 38.798 183 101 4 70 241 163 345 1.45e-36 135
MsG0480021792.01.T01 MTR_1g022160 38.798 183 101 4 70 241 163 345 1.82e-36 135
MsG0480021792.01.T01 MTR_1g022160 39.891 183 99 5 70 241 163 345 1.87e-36 135
MsG0480021792.01.T01 MTR_4g069940 37.989 179 101 3 71 239 169 347 4.93e-36 133
MsG0480021792.01.T01 MTR_3g065460 36.559 186 105 3 70 242 162 347 5.75e-36 134
MsG0480021792.01.T01 MTR_2g019345 41.573 178 94 4 71 238 162 339 6.92e-36 132
MsG0480021792.01.T01 MTR_7g026340 39.691 194 100 6 71 254 162 348 1.00e-35 132
MsG0480021792.01.T01 MTR_4g114850 36.559 186 108 4 68 243 163 348 1.43e-35 130
MsG0480021792.01.T01 MTR_4g114850 36.559 186 108 4 68 243 163 348 1.55e-35 130
MsG0480021792.01.T01 MTR_4g114850 36.559 186 108 4 68 243 163 348 1.68e-35 130
MsG0480021792.01.T01 MTR_3g116510 36.464 181 105 3 68 238 31 211 5.49e-35 128
MsG0480021792.01.T01 MTR_4g036775 57.143 98 42 0 143 240 80 177 6.09e-35 125
MsG0480021792.01.T01 MTR_4g036680 57.143 98 42 0 143 240 80 177 6.29e-35 125
MsG0480021792.01.T01 MTR_4g083500 42.857 161 80 5 89 239 202 360 1.36e-34 130
MsG0480021792.01.T01 MTR_3g116510 36.464 181 105 3 68 238 165 345 4.97e-34 128
MsG0480021792.01.T01 MTR_4g132280 36.216 185 105 3 72 243 185 369 9.14e-33 125
MsG0480021792.01.T01 MTR_3g083580 35.882 170 96 3 72 228 166 335 3.64e-31 120
MsG0480021792.01.T01 MTR_7g085390 35.593 177 104 5 74 242 174 348 1.79e-28 113
MsG0480021792.01.T01 MTR_8g085720 32.609 184 111 3 72 242 166 349 1.93e-27 110
MsG0480021792.01.T01 MTR_4g112025 36.612 183 102 6 74 246 180 358 1.95e-27 110
MsG0480021792.01.T01 MTR_3g095050 37.853 177 100 5 74 242 179 353 2.19e-27 110
MsG0480021792.01.T01 MTR_8g092070 34.807 181 108 5 70 242 177 355 5.03e-27 109
MsG0480021792.01.T01 MTR_6g032820 37.222 180 101 6 70 239 172 349 1.26e-26 107
MsG0480021792.01.T01 MTR_3g031770 36.905 168 95 6 79 238 178 342 5.47e-26 106
MsG0480021792.01.T01 MTR_2g027560 34.555 191 109 7 74 251 175 362 1.07e-25 105
MsG0480021792.01.T01 MTR_4g134280 34.031 191 110 7 74 251 58 245 1.96e-25 103
MsG0480021792.01.T01 MTR_3g108600 36.994 173 99 6 74 238 159 329 9.11e-24 99.8
MsG0480021792.01.T01 MTR_1g053320 32.768 177 110 4 74 242 179 354 6.05e-23 97.4
MsG0480021792.01.T01 MTR_1g010120 34.104 173 104 5 74 238 173 343 1.70e-22 96.3
MsG0480021792.01.T01 MTR_4g124440 32.768 177 109 5 74 242 175 349 1.80e-22 96.3
MsG0480021792.01.T01 MTR_5g081720 32.955 176 103 6 72 236 177 348 2.03e-20 89.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021792.01.T01 AT4G16260 51.934 181 80 3 68 242 128 307 3.36e-59 190
MsG0480021792.01.T01 AT3G57260 55.882 170 70 3 73 238 184 352 3.92e-59 191
MsG0480021792.01.T01 AT3G57260 55.882 170 70 3 73 238 168 336 4.07e-59 191
MsG0480021792.01.T01 AT4G16260 51.934 181 80 3 68 242 157 336 6.35e-59 191
MsG0480021792.01.T01 AT3G57240 54.913 173 70 2 72 238 168 338 1.02e-58 190
MsG0480021792.01.T01 AT3G57270 52.601 173 77 2 71 238 165 337 9.80e-55 179
MsG0480021792.01.T01 AT5G56590 40.437 183 98 3 71 242 166 348 4.89e-44 155
MsG0480021792.01.T01 AT2G05790 43.889 180 90 4 70 238 163 342 1.08e-43 154
MsG0480021792.01.T01 AT5G55180 43.011 186 95 5 68 242 163 348 3.16e-43 152
MsG0480021792.01.T01 AT5G55180 40.594 202 109 5 68 258 163 364 3.62e-43 152
MsG0480021792.01.T01 AT5G20340 44.318 176 89 5 71 238 179 353 5.80e-43 149
MsG0480021792.01.T01 AT4G26830 42.857 182 93 4 68 238 164 345 7.09e-43 151
MsG0480021792.01.T01 AT2G01630 41.398 186 96 3 70 242 161 346 7.59e-43 150
MsG0480021792.01.T01 AT4G26830 42.857 182 93 4 68 238 160 341 8.42e-43 151
MsG0480021792.01.T01 AT2G01630 41.398 186 96 3 70 242 161 346 2.65e-42 149
MsG0480021792.01.T01 AT5G20390 40.761 184 100 5 63 238 161 343 7.09e-42 146
MsG0480021792.01.T01 AT2G01630 41.398 186 96 3 70 242 161 346 1.02e-41 149
MsG0480021792.01.T01 AT5G20330 42.391 184 97 5 63 238 162 344 1.55e-41 145
MsG0480021792.01.T01 AT2G16230 40.659 182 98 3 68 239 162 343 2.78e-41 148
MsG0480021792.01.T01 AT2G27500 37.745 204 113 6 68 257 88 291 4.31e-41 144
MsG0480021792.01.T01 AT3G15800 39.037 187 104 3 68 244 181 367 4.83e-41 145
MsG0480021792.01.T01 AT2G26600 34.673 199 118 5 65 251 72 270 1.64e-40 141
MsG0480021792.01.T01 AT2G27500 37.745 204 113 6 68 257 166 369 2.29e-40 144
MsG0480021792.01.T01 AT2G27500 37.129 202 114 5 68 256 166 367 3.25e-40 143
MsG0480021792.01.T01 AT2G26600 34.673 199 118 5 65 251 166 364 1.81e-39 141
MsG0480021792.01.T01 AT5G42100 43.646 181 89 5 71 238 166 346 8.46e-39 140
MsG0480021792.01.T01 AT5G42100 43.646 181 89 5 71 238 166 346 9.12e-39 140
MsG0480021792.01.T01 AT1G66250 42.135 178 89 4 72 236 172 348 9.21e-39 141
MsG0480021792.01.T01 AT1G77780 38.798 183 104 4 63 238 151 332 3.15e-38 137
MsG0480021792.01.T01 AT5G42720 38.674 181 101 3 68 238 164 344 2.10e-37 137
MsG0480021792.01.T01 AT1G33220 41.379 174 94 4 63 228 162 335 6.89e-37 133
MsG0480021792.01.T01 AT1G11820 36.702 188 104 4 70 242 182 369 8.50e-37 134
MsG0480021792.01.T01 AT1G11820 36.634 202 111 5 70 254 182 383 1.75e-36 135
MsG0480021792.01.T01 AT4G14080 38.710 186 101 4 70 242 180 365 2.22e-36 134
MsG0480021792.01.T01 AT4G18340 39.286 196 109 5 68 253 170 365 2.83e-36 132
MsG0480021792.01.T01 AT4G18340 39.286 196 109 5 68 253 170 365 2.96e-36 133
MsG0480021792.01.T01 AT1G11820 36.634 202 111 5 70 254 182 383 3.42e-36 134
MsG0480021792.01.T01 AT4G29360 41.290 155 81 4 84 228 189 343 4.35e-36 134
MsG0480021792.01.T01 AT4G29360 41.290 155 81 4 84 228 179 333 4.84e-36 134
MsG0480021792.01.T01 AT3G23770 40.323 186 98 4 70 242 178 363 7.48e-36 133
MsG0480021792.01.T01 AT1G77790 38.764 178 99 4 70 238 165 341 1.68e-35 130
MsG0480021792.01.T01 AT4G34480 40.741 162 86 3 87 238 182 343 4.85e-35 129
MsG0480021792.01.T01 AT5G24318 36.500 200 110 6 52 238 149 344 6.91e-35 129
MsG0480021792.01.T01 AT5G24318 37.569 181 98 5 70 239 30 206 7.51e-35 127
MsG0480021792.01.T01 AT4G34480 40.741 162 86 3 87 238 71 232 9.39e-35 129
MsG0480021792.01.T01 AT1G32860 38.674 181 99 5 70 238 167 347 1.03e-34 129
MsG0480021792.01.T01 AT3G07320 35.829 187 106 4 70 242 163 349 1.37e-34 129
MsG0480021792.01.T01 AT3G61810 37.989 179 92 5 72 236 194 367 3.36e-34 127
MsG0480021792.01.T01 AT4G34480 40.741 162 86 3 87 238 182 343 3.84e-34 129
MsG0480021792.01.T01 AT5G24318 37.569 181 98 5 70 239 169 345 3.97e-34 128
MsG0480021792.01.T01 AT5G24318 36.318 201 111 6 52 239 149 345 4.24e-34 128
MsG0480021792.01.T01 AT1G30080 37.430 179 102 5 71 239 175 353 6.89e-33 124
MsG0480021792.01.T01 AT1G30080 37.430 179 102 5 71 239 175 353 7.71e-33 123
MsG0480021792.01.T01 AT3G46570 41.104 163 76 6 89 238 187 342 3.02e-31 119
MsG0480021792.01.T01 AT5G20560 36.970 165 96 4 73 230 173 336 5.15e-31 117
MsG0480021792.01.T01 AT3G13560 33.708 178 105 3 72 236 166 343 1.14e-30 119
MsG0480021792.01.T01 AT3G13560 33.708 178 105 3 72 236 166 343 1.14e-30 119
MsG0480021792.01.T01 AT3G13560 33.708 178 105 3 72 236 166 343 1.14e-30 119
MsG0480021792.01.T01 AT3G13560 33.708 178 105 3 72 236 166 343 1.14e-30 119
MsG0480021792.01.T01 AT3G13560 33.708 178 105 3 72 236 166 343 1.14e-30 119
MsG0480021792.01.T01 AT5G18220 37.853 177 99 6 74 242 176 349 5.40e-29 114
MsG0480021792.01.T01 AT3G04010 38.150 173 96 6 74 238 181 350 2.38e-27 110
MsG0480021792.01.T01 AT2G19440 35.227 176 105 5 74 242 169 342 1.88e-26 107
MsG0480021792.01.T01 AT2G19440 35.227 176 105 5 74 242 169 342 1.88e-26 107
MsG0480021792.01.T01 AT1G64760 35.227 176 105 5 74 242 173 346 2.82e-26 107
MsG0480021792.01.T01 AT1G64760 35.227 176 105 5 74 242 173 346 2.82e-26 107
MsG0480021792.01.T01 AT5G58090 36.842 171 98 5 74 236 169 337 5.12e-26 106
MsG0480021792.01.T01 AT3G24330 33.898 177 108 4 74 242 187 362 6.24e-26 106
MsG0480021792.01.T01 AT4G31140 36.207 174 101 5 74 239 174 345 1.06e-25 105
MsG0480021792.01.T01 AT5G58480 33.728 169 105 3 74 236 174 341 3.41e-25 103
MsG0480021792.01.T01 AT4G17180 34.659 176 101 6 74 238 165 337 7.30e-25 103
MsG0480021792.01.T01 AT2G39640 33.140 172 104 4 74 238 171 338 2.38e-24 102
MsG0480021792.01.T01 AT3G55780 31.492 181 106 6 70 236 173 349 2.45e-24 100
MsG0480021792.01.T01 AT5G64790 33.333 177 108 5 74 242 175 349 3.36e-24 101
MsG0480021792.01.T01 AT3G55430 36.420 162 89 5 87 239 186 342 7.32e-24 100
MsG0480021792.01.T01 AT5G20870 30.387 181 109 6 74 239 177 355 3.03e-18 84.0

Find 57 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCATCTTAAATATGAAAT+AGG 0.232338 4:+64325811 MsG0480021792.01.T01:CDS
TTCACTAGGAAGTTAATTAT+TGG 0.271826 4:-64325320 None:intergenic
AAATATAAACAGAGGCAATT+TGG 0.302939 4:+64325239 MsG0480021792.01.T01:intron
GACTGTGCCACATGGTATTT+AGG 0.330651 4:-64325755 None:intergenic
GCTTCTATTGCATTGAGATA+TGG 0.347217 4:-64323659 None:intergenic
AGAAATGCCTTGTTTATTGC+TGG 0.349560 4:-64325390 None:intergenic
GCATTGAGATATGGTACTTC+AGG 0.358055 4:-64323650 None:intergenic
ATAGCAGCACCGGGCCTCAT+AGG 0.417015 4:-64325641 None:intergenic
GTCGTATATATGAATTTGCT+TGG 0.436691 4:-64325298 None:intergenic
CTTTGACGCCATGTTAGATT+CGG 0.437617 4:+64325469 MsG0480021792.01.T01:CDS
GTCAGTGAAAGCTCCATCAT+TGG 0.440169 4:-64325276 None:intergenic
ATTAACTTCCTAGTGAAGAA+TGG 0.481075 4:+64325326 MsG0480021792.01.T01:CDS
TGGTATTTAGGTGATTTGTC+AGG 0.481833 4:-64325743 None:intergenic
GTAAGTTTCAATAGCAGCAC+CGG 0.508709 4:-64325651 None:intergenic
CTAGCAACTGAACAACACTA+TGG 0.509757 4:+64325710 MsG0480021792.01.T01:CDS
TTGATCAATCACATCAAGAA+TGG 0.511422 4:+64325614 MsG0480021792.01.T01:CDS
TTTGATGAAAATCAGAAGAC+TGG 0.512681 4:+64325686 MsG0480021792.01.T01:CDS
TCAGTGAAAGCTCCATCATT+GGG 0.513093 4:-64325275 None:intergenic
AATTCATATCCACCCAATGA+TGG 0.515028 4:+64325263 MsG0480021792.01.T01:CDS
TACGCTGCTCTCGAGAAAGT+TGG 0.518967 4:+64325494 MsG0480021792.01.T01:CDS
CAACAAGGAAACAATGCAGT+TGG 0.521019 4:+64325434 MsG0480021792.01.T01:CDS
GAGAGTGGATGGCCGTCTAA+AGG 0.521537 4:+64325545 MsG0480021792.01.T01:CDS
TAAATACCATGTGGCACAGT+CGG 0.536931 4:+64325757 MsG0480021792.01.T01:CDS
AACAAGGAAACAATGCAGTT+GGG 0.544475 4:+64325435 MsG0480021792.01.T01:CDS
GTTCTTCTCCCGTCTTCTTG+AGG 0.546080 4:-64323619 None:intergenic
TCAAGAATGGCACTCCTATG+AGG 0.547579 4:+64325627 MsG0480021792.01.T01:CDS
CCGTCTTCTTGAGGTCTGAA+AGG 0.554529 4:-64323610 None:intergenic
AAATACCATGTGGCACAGTC+GGG 0.556287 4:+64325758 MsG0480021792.01.T01:CDS
CTTTCAGACCTCAAGAAGAC+GGG 0.561230 4:+64323611 MsG0480021792.01.T01:CDS
GCTGCTCTCGAGAAAGTTGG+AGG 0.568267 4:+64325497 MsG0480021792.01.T01:CDS
TTTAGACGGCCATCCACTCT+CGG 0.574351 4:-64325543 None:intergenic
GGCACAGTCGGGTATCGAGT+TGG 0.574353 4:+64325769 MsG0480021792.01.T01:CDS
TAGTTGTGTCCGAGAGTGGA+TGG 0.576028 4:+64325534 MsG0480021792.01.T01:CDS
AATGGCACTCCTATGAGGCC+CGG 0.577050 4:+64325632 MsG0480021792.01.T01:CDS
CTTCACCTACACGAAGACAT+TGG 0.584686 4:+64323753 MsG0480021792.01.T01:CDS
CCTTTCAGACCTCAAGAAGA+CGG 0.585243 4:+64323610 MsG0480021792.01.T01:CDS
AAGATAGTTGTGTCCGAGAG+TGG 0.586296 4:+64325530 MsG0480021792.01.T01:CDS
GATCAAATTAGAATAATATG+TGG 0.597633 4:-64325597 None:intergenic
GTGGTGCTCCATTCTTCACT+AGG 0.601052 4:-64325334 None:intergenic
CCATTCCAATGTCTTCGTGT+AGG 0.607553 4:-64323758 None:intergenic
GGATACACATTGGCAAGAAG+TGG 0.610222 4:-64325353 None:intergenic
AAGACGGGAGAAGAACTACT+TGG 0.611724 4:+64323626 MsG0480021792.01.T01:CDS
GCACTATCTCCTCCTTTAGA+CGG 0.613085 4:-64325557 None:intergenic
AGCGTATACCGAATCTAACA+TGG 0.613507 4:-64325477 None:intergenic
TGTGGCAGCATTGTCAAATG+AGG 0.614053 4:-64325579 None:intergenic
CGATACCCGACTGTGCCACA+TGG 0.618586 4:-64325763 None:intergenic
TTGCTGGTATAAGAGAAGTA+CGG 0.630124 4:-64325374 None:intergenic
GGACACAACTATCTTCACAT+TGG 0.633314 4:-64325522 None:intergenic
AGTGGATGGCCGTCTAAAGG+AGG 0.638373 4:+64325548 MsG0480021792.01.T01:CDS
TAAGTTTCAATAGCAGCACC+GGG 0.642794 4:-64325650 None:intergenic
AATCTAACATGGCGTCAAAG+AGG 0.650822 4:-64325466 None:intergenic
CCTACACGAAGACATTGGAA+TGG 0.651898 4:+64323758 MsG0480021792.01.T01:CDS
AGAGAAGTACGGATACACAT+TGG 0.664741 4:-64325363 None:intergenic
GTGAAAGCTCCATCATTGGG+TGG 0.665539 4:-64325272 None:intergenic
TCTTATACCAGCAATAAACA+AGG 0.677661 4:+64325383 MsG0480021792.01.T01:CDS
CAAATCACCTAAATACCATG+TGG 0.698839 4:+64325748 MsG0480021792.01.T01:CDS
GTATCGAGTTGGATACAACA+AGG 0.731881 4:+64325780 MsG0480021792.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATATTCGTATTTATT+AGG - Chr4:64325117-64325136 None:intergenic 10.0%
!! TTTGTCTAAAAAAATAAAAA+TGG - Chr4:64324716-64324735 None:intergenic 10.0%
!!! ATAAATACGAATATTTTTTT+TGG + Chr4:64325118-64325137 MsG0480021792.01.T01:intron 10.0%
!!! GTTTTGGAATTTTATATTTT+GGG - Chr4:64324852-64324871 None:intergenic 15.0%
!! ACAAATGAGTTCTATTTATA+GGG - Chr4:64324591-64324610 None:intergenic 20.0%
!! CAACATATACAAAATAATTG+TGG + Chr4:64323995-64324014 MsG0480021792.01.T01:intron 20.0%
!! CTTAAATAAACAACAAGATA+TGG - Chr4:64324822-64324841 None:intergenic 20.0%
!! GAGTGTTATAAATATTATAG+TGG + Chr4:64324492-64324511 MsG0480021792.01.T01:intron 20.0%
!! GATCAAATTAGAATAATATG+TGG - Chr4:64325600-64325619 None:intergenic 20.0%
!! TACAAATGAGTTCTATTTAT+AGG - Chr4:64324592-64324611 None:intergenic 20.0%
!! TTCCTAATAAATATAAACAG+AGG + Chr4:64325231-64325250 MsG0480021792.01.T01:intron 20.0%
!! TTTGTAGAAGAATAAAAAAG+TGG - Chr4:64324773-64324792 None:intergenic 20.0%
!!! ATTTTAGTAAGGTTTTAATG+AGG + Chr4:64324443-64324462 MsG0480021792.01.T01:intron 20.0%
!!! CGTTTTGGAATTTTATATTT+TGG - Chr4:64324853-64324872 None:intergenic 20.0%
! AAATATAAACAGAGGCAATT+TGG + Chr4:64325239-64325258 MsG0480021792.01.T01:intron 25.0%
! AATAATTGTGGTTTATCTTC+TGG + Chr4:64324007-64324026 MsG0480021792.01.T01:intron 25.0%
! AGTTTCTCAAAAACTTTACT+TGG - Chr4:64324230-64324249 None:intergenic 25.0%
! ATATACATATCTCCATGAAA+TGG - Chr4:64323885-64323904 None:intergenic 25.0%
! GAGAAACTTGTATTAAGATT+TGG + Chr4:64324242-64324261 MsG0480021792.01.T01:intron 25.0%
! GCCAAAAAAAATATTCGTTT+GGG - Chr4:64325003-64325022 None:intergenic 25.0%
! GTTTCTCAAAAACTTTACTT+GGG - Chr4:64324229-64324248 None:intergenic 25.0%
! TAGAAACAATAATAGAGAGA+AGG - Chr4:64324670-64324689 None:intergenic 25.0%
! TGCCAAAAAAAATATTCGTT+TGG - Chr4:64325004-64325023 None:intergenic 25.0%
! TGCCTCTGTTTATATTTATT+AGG - Chr4:64325236-64325255 None:intergenic 25.0%
! TGTAACATTCTCAATAACAT+TGG + Chr4:64324608-64324627 MsG0480021792.01.T01:intron 25.0%
! TTACTAAAATAAAACCCAGT+GGG - Chr4:64324433-64324452 None:intergenic 25.0%
! TTCACTAGGAAGTTAATTAT+TGG - Chr4:64325323-64325342 None:intergenic 25.0%
! TTGAAAGAAGGATACATTAA+AGG + Chr4:64324082-64324101 MsG0480021792.01.T01:intron 25.0%
!! TATTCGACAAAATAACGTTT+TGG - Chr4:64324868-64324887 None:intergenic 25.0%
!!! ACCCAAACGAATATTTTTTT+TGG + Chr4:64324999-64325018 MsG0480021792.01.T01:intron 25.0%
!!! CAAATAGTAAATGGGTTAAT+GGG + Chr4:64324520-64324539 MsG0480021792.01.T01:intron 25.0%
!!! CTTGTTGTTTATTTAAGCTT+GGG + Chr4:64324825-64324844 MsG0480021792.01.T01:intron 25.0%
!!! TCTTGTTGTTTATTTAAGCT+TGG + Chr4:64324824-64324843 MsG0480021792.01.T01:intron 25.0%
ATTAACTTCCTAGTGAAGAA+TGG + Chr4:64325326-64325345 MsG0480021792.01.T01:CDS 30.0%
CCATTAACCCATTTACTATT+TGG - Chr4:64324522-64324541 None:intergenic 30.0%
CTTACTAAAATAAAACCCAG+TGG - Chr4:64324434-64324453 None:intergenic 30.0%
GTAGAGAATGTGTTTGATTA+AGG + Chr4:64323963-64323982 MsG0480021792.01.T01:intron 30.0%
GTGGGAAAAAATTATAGTGA+AGG - Chr4:64324415-64324434 None:intergenic 30.0%
TAGGGAAATAGAATAGCTTA+TGG - Chr4:64324573-64324592 None:intergenic 30.0%
TATTTGTCAAATCCTCATCA+TGG + Chr4:64323818-64323837 MsG0480021792.01.T01:intron 30.0%
TCTTATACCAGCAATAAACA+AGG + Chr4:64325383-64325402 MsG0480021792.01.T01:CDS 30.0%
TGGATGTCCAAATAGTAAAT+GGG + Chr4:64324512-64324531 MsG0480021792.01.T01:intron 30.0%
TTGATCAATCACATCAAGAA+TGG + Chr4:64325614-64325633 MsG0480021792.01.T01:CDS 30.0%
TTTGATGAAAATCAGAAGAC+TGG + Chr4:64325686-64325705 MsG0480021792.01.T01:CDS 30.0%
! CTTCTGATTTTCATCAAACA+TGG - Chr4:64325684-64325703 None:intergenic 30.0%
! GTCGTATATATGAATTTGCT+TGG - Chr4:64325301-64325320 None:intergenic 30.0%
! TATGCTCTTTTTAGACAACA+AGG + Chr4:64325419-64325438 MsG0480021792.01.T01:CDS 30.0%
!! ATTTGATGATGTTGCTGTTA+TGG - Chr4:64323912-64323931 None:intergenic 30.0%
!! CCAAATAGTAAATGGGTTAA+TGG + Chr4:64324519-64324538 MsG0480021792.01.T01:intron 30.0%
!! TGGGAGAAATATGTTTTGTT+TGG - Chr4:64324111-64324130 None:intergenic 30.0%
!!! AAAATTTTTAGCACTTCACG+AGG + Chr4:64323937-64323956 MsG0480021792.01.T01:intron 30.0%
!!! CACTATAATTTTTTCCCACT+GGG + Chr4:64324416-64324435 MsG0480021792.01.T01:intron 30.0%
!!! CACTGGGTTTTATTTTAGTA+AGG + Chr4:64324432-64324451 MsG0480021792.01.T01:intron 30.0%
!!! TCACTATAATTTTTTCCCAC+TGG + Chr4:64324415-64324434 MsG0480021792.01.T01:intron 30.0%
AACAAGGAAACAATGCAGTT+GGG + Chr4:64325435-64325454 MsG0480021792.01.T01:CDS 35.0%
AATTCATATCCACCCAATGA+TGG + Chr4:64325263-64325282 MsG0480021792.01.T01:CDS 35.0%
ATATTTATAACACTCCCCCT+TGG - Chr4:64324487-64324506 None:intergenic 35.0%
ATCACGGGTGAAAAAGAATT+TGG - Chr4:64324131-64324150 None:intergenic 35.0%
CAAATCACCTAAATACCATG+TGG + Chr4:64325748-64325767 MsG0480021792.01.T01:CDS 35.0%
CCCTCATGAAGACAAATATA+CGG - Chr4:64324274-64324293 None:intergenic 35.0%
CCGTATATTTGTCTTCATGA+GGG + Chr4:64324271-64324290 MsG0480021792.01.T01:intron 35.0%
CGTATATTTGTCTTCATGAG+GGG + Chr4:64324272-64324291 MsG0480021792.01.T01:intron 35.0%
GCTTCTATTGCATTGAGATA+TGG - Chr4:64323662-64323681 None:intergenic 35.0%
GGCTATACTACTAGATCATT+GGG + Chr4:64324541-64324560 MsG0480021792.01.T01:intron 35.0%
GTATATTTGTCTTCATGAGG+GGG + Chr4:64324273-64324292 MsG0480021792.01.T01:intron 35.0%
GTGGATGTCCAAATAGTAAA+TGG + Chr4:64324511-64324530 MsG0480021792.01.T01:intron 35.0%
TCACGGGTGAAAAAGAATTT+GGG - Chr4:64324130-64324149 None:intergenic 35.0%
TCATCATGGTAGATCACAAA+CGG + Chr4:64323832-64323851 MsG0480021792.01.T01:intron 35.0%
TCCGTATATTTGTCTTCATG+AGG + Chr4:64324270-64324289 MsG0480021792.01.T01:intron 35.0%
TGTATTGCTCAGTTGAAAGA+AGG + Chr4:64324070-64324089 MsG0480021792.01.T01:intron 35.0%
TTGCTGGTATAAGAGAAGTA+CGG - Chr4:64325377-64325396 None:intergenic 35.0%
! TGGTATTTAGGTGATTTGTC+AGG - Chr4:64325746-64325765 None:intergenic 35.0%
! TGGTTTATCTTCTGGAAGAA+TGG + Chr4:64324015-64324034 MsG0480021792.01.T01:intron 35.0%
!! ACCATTCTTTTGAAGATCAC+GGG - Chr4:64324146-64324165 None:intergenic 35.0%
!! AGAAATGCCTTGTTTATTGC+TGG - Chr4:64325393-64325412 None:intergenic 35.0%
!! CACCATTCTTTTGAAGATCA+CGG - Chr4:64324147-64324166 None:intergenic 35.0%
AATCTAACATGGCGTCAAAG+AGG - Chr4:64325469-64325488 None:intergenic 40.0%
ACCCGTGATCTTCAAAAGAA+TGG + Chr4:64324142-64324161 MsG0480021792.01.T01:intron 40.0%
AGCGTATACCGAATCTAACA+TGG - Chr4:64325480-64325499 None:intergenic 40.0%
CAACAAGGAAACAATGCAGT+TGG + Chr4:64325434-64325453 MsG0480021792.01.T01:CDS 40.0%
CATCATGGTAGATCACAAAC+GGG + Chr4:64323833-64323852 MsG0480021792.01.T01:intron 40.0%
CTAGCAACTGAACAACACTA+TGG + Chr4:64325710-64325729 MsG0480021792.01.T01:CDS 40.0%
CTTTGACGCCATGTTAGATT+CGG + Chr4:64325469-64325488 MsG0480021792.01.T01:CDS 40.0%
GCATTGAGATATGGTACTTC+AGG - Chr4:64323653-64323672 None:intergenic 40.0%
GGACACAACTATCTTCACAT+TGG - Chr4:64325525-64325544 None:intergenic 40.0%
GGGCTATACTACTAGATCAT+TGG + Chr4:64324540-64324559 MsG0480021792.01.T01:intron 40.0%
GTAAGTTTCAATAGCAGCAC+CGG - Chr4:64325654-64325673 None:intergenic 40.0%
GTGGAGATACAACCATTTCA+TGG + Chr4:64323870-64323889 MsG0480021792.01.T01:intron 40.0%
GTTTGTGATCTACCATGATG+AGG - Chr4:64323833-64323852 None:intergenic 40.0%
TAAATACCATGTGGCACAGT+CGG + Chr4:64325757-64325776 MsG0480021792.01.T01:CDS 40.0%
TAAGTTTCAATAGCAGCACC+GGG - Chr4:64325653-64325672 None:intergenic 40.0%
TCAGTGAAAGCTCCATCATT+GGG - Chr4:64325278-64325297 None:intergenic 40.0%
TTAATGAGGCATATCCTCGA+TGG + Chr4:64324457-64324476 MsG0480021792.01.T01:intron 40.0%
! ACGGGTTACTTGTTTACAAG+TGG + Chr4:64323851-64323870 MsG0480021792.01.T01:intron 40.0%
! AGAGAAGTACGGATACACAT+TGG - Chr4:64325366-64325385 None:intergenic 40.0%
! TCCTGGTAAGGTTTTTAACG+AGG + Chr4:64324355-64324374 MsG0480021792.01.T01:intron 40.0%
!! GTATCGAGTTGGATACAACA+AGG + Chr4:64325780-64325799 MsG0480021792.01.T01:CDS 40.0%
!!! CACTCTTTTTTCCTTCACCA+AGG + Chr4:64324314-64324333 MsG0480021792.01.T01:intron 40.0%
AAATACCATGTGGCACAGTC+GGG + Chr4:64325758-64325777 MsG0480021792.01.T01:CDS 45.0%
AACCCAATGGGACAAAACCT+TGG - Chr4:64324334-64324353 None:intergenic 45.0%
AAGACGGGAGAAGAACTACT+TGG + Chr4:64323626-64323645 MsG0480021792.01.T01:CDS 45.0%
AAGATAGTTGTGTCCGAGAG+TGG + Chr4:64325530-64325549 MsG0480021792.01.T01:CDS 45.0%
ACCTTACCAGGAAAACCCAA+TGG - Chr4:64324347-64324366 None:intergenic 45.0%
ATATCCTCGATGGACATCCA+AGG + Chr4:64324467-64324486 MsG0480021792.01.T01:intron 45.0%
ATGGGACAAAACCTTGGTGA+AGG - Chr4:64324328-64324347 None:intergenic 45.0%
CCATTCCAATGTCTTCGTGT+AGG - Chr4:64323761-64323780 None:intergenic 45.0%
CCTACACGAAGACATTGGAA+TGG + Chr4:64323758-64323777 MsG0480021792.01.T01:CDS 45.0%
CCTTACCAGGAAAACCCAAT+GGG - Chr4:64324346-64324365 None:intergenic 45.0%
CCTTTCAGACCTCAAGAAGA+CGG + Chr4:64323610-64323629 MsG0480021792.01.T01:CDS 45.0%
CTTCACCTACACGAAGACAT+TGG + Chr4:64323753-64323772 MsG0480021792.01.T01:CDS 45.0%
CTTTCAGACCTCAAGAAGAC+GGG + Chr4:64323611-64323630 MsG0480021792.01.T01:CDS 45.0%
GACTGTGCCACATGGTATTT+AGG - Chr4:64325758-64325777 None:intergenic 45.0%
GCACTATCTCCTCCTTTAGA+CGG - Chr4:64325560-64325579 None:intergenic 45.0%
GCCTCGTTAAAAACCTTACC+AGG - Chr4:64324359-64324378 None:intergenic 45.0%
GGATACACATTGGCAAGAAG+TGG - Chr4:64325356-64325375 None:intergenic 45.0%
GTCAGTGAAAGCTCCATCAT+TGG - Chr4:64325279-64325298 None:intergenic 45.0%
TATCCTCGATGGACATCCAA+GGG + Chr4:64324468-64324487 MsG0480021792.01.T01:intron 45.0%
TCAAGAATGGCACTCCTATG+AGG + Chr4:64325627-64325646 MsG0480021792.01.T01:CDS 45.0%
TGTGGCAGCATTGTCAAATG+AGG - Chr4:64325582-64325601 None:intergenic 45.0%
! GGTGAAGGAAAAAAGAGTGC+AGG - Chr4:64324313-64324332 None:intergenic 45.0%
! TTTGTCCCATTGGGTTTTCC+TGG + Chr4:64324338-64324357 MsG0480021792.01.T01:intron 45.0%
!! CACCAAGGTTTTGTCCCATT+GGG + Chr4:64324329-64324348 MsG0480021792.01.T01:intron 45.0%
!! TCACCAAGGTTTTGTCCCAT+TGG + Chr4:64324328-64324347 MsG0480021792.01.T01:intron 45.0%
ATCCTCGATGGACATCCAAG+GGG + Chr4:64324469-64324488 MsG0480021792.01.T01:intron 50.0%
CCGTCTTCTTGAGGTCTGAA+AGG - Chr4:64323613-64323632 None:intergenic 50.0%
GTGAAAGCTCCATCATTGGG+TGG - Chr4:64325275-64325294 None:intergenic 50.0%
GTGGTGCTCCATTCTTCACT+AGG - Chr4:64325337-64325356 None:intergenic 50.0%
GTTCTTCTCCCGTCTTCTTG+AGG - Chr4:64323622-64323641 None:intergenic 50.0%
TACGCTGCTCTCGAGAAAGT+TGG + Chr4:64325494-64325513 MsG0480021792.01.T01:CDS 50.0%
TAGTTGTGTCCGAGAGTGGA+TGG + Chr4:64325534-64325553 MsG0480021792.01.T01:CDS 50.0%
TTTAGACGGCCATCCACTCT+CGG - Chr4:64325546-64325565 None:intergenic 50.0%
! CCCATTGGGTTTTCCTGGTA+AGG + Chr4:64324343-64324362 MsG0480021792.01.T01:intron 50.0%
AATGGCACTCCTATGAGGCC+CGG + Chr4:64325632-64325651 MsG0480021792.01.T01:CDS 55.0%
AGTGGATGGCCGTCTAAAGG+AGG + Chr4:64325548-64325567 MsG0480021792.01.T01:CDS 55.0%
CGAGGCAGTTCGCACTCAAA+AGG + Chr4:64324373-64324392 MsG0480021792.01.T01:intron 55.0%
GAGAGTGGATGGCCGTCTAA+AGG + Chr4:64325545-64325564 MsG0480021792.01.T01:CDS 55.0%
GCTGCTCTCGAGAAAGTTGG+AGG + Chr4:64325497-64325516 MsG0480021792.01.T01:CDS 55.0%
TCCTCGATGGACATCCAAGG+GGG + Chr4:64324470-64324489 MsG0480021792.01.T01:intron 55.0%
CGATACCCGACTGTGCCACA+TGG - Chr4:64325766-64325785 None:intergenic 60.0%
TCCCCCTTGGATGTCCATCG+AGG - Chr4:64324474-64324493 None:intergenic 60.0%
! ATAGCAGCACCGGGCCTCAT+AGG - Chr4:64325644-64325663 None:intergenic 60.0%
!! GGCACAGTCGGGTATCGAGT+TGG + Chr4:64325769-64325788 MsG0480021792.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 64323596 64325832 64323596 ID=MsG0480021792.01;Name=MsG0480021792.01
Chr4 mRNA 64323596 64325832 64323596 ID=MsG0480021792.01.T01;Parent=MsG0480021792.01;Name=MsG0480021792.01.T01;_AED=0.38;_eAED=0.38;_QI=0|0|0|0.5|1|1|2|0|258
Chr4 exon 64323596 64323790 64323596 ID=MsG0480021792.01.T01:exon:7762;Parent=MsG0480021792.01.T01
Chr4 exon 64325251 64325832 64325251 ID=MsG0480021792.01.T01:exon:7763;Parent=MsG0480021792.01.T01
Chr4 CDS 64323596 64323790 64323596 ID=MsG0480021792.01.T01:cds;Parent=MsG0480021792.01.T01
Chr4 CDS 64325251 64325832 64325251 ID=MsG0480021792.01.T01:cds;Parent=MsG0480021792.01.T01
Gene Sequence

>MsG0480021792.01.T01

ATGTGGAAAAAGATCCTTTCAGACCTCAAGAAGACGGGAGAAGAACTACTTGGTCCTGAAGTACCATATCTCAATGCAATAGAAGCATTGATGTATCTTGCTAATTATACACGTACTGATATATCATTTTCTGTCAATTTATTATCAAGATATAGTTCTTCACCTACACGAAGACATTGGAATGGAGTTAAACATAGGCAATTTGGTAATTCATATCCACCCAATGATGGAGCTTTCACTGACCAAGCAAATTCATATATACGACCAATAATTAACTTCCTAGTGAAGAATGGAGCACCACTTCTTGCCAATGTGTATCCGTACTTCTCTTATACCAGCAATAAACAAGGCATTTCTCTTGATTATGCTCTTTTTAGACAACAAGGAAACAATGCAGTTGGGTACAACAACCTCTTTGACGCCATGTTAGATTCGGTATACGCTGCTCTCGAGAAAGTTGGAGGTTCCAATGTGAAGATAGTTGTGTCCGAGAGTGGATGGCCGTCTAAAGGAGGAGATAGTGCCTCATTTGACAATGCTGCCACATATTATTCTAATTTGATCAATCACATCAAGAATGGCACTCCTATGAGGCCCGGTGCTGCTATTGAAACTTACTTGTTTGCCATGTTTGATGAAAATCAGAAGACTGGTCTAGCAACTGAACAACACTATGGTCTCTTTAATCCTGACAAATCACCTAAATACCATGTGGCACAGTCGGGTATCGAGTTGGATACAACAAGGAACATTACTGTCATCTTAAATATGAAATAG
Protein sequence

>MsG0480021792.01.T01

MWKKILSDLKKTGEELLGPEVPYLNAIEALMYLANYTRTDISFSVNLLSRYSSSPTRRHWNGVKHRQFGNSYPPNDGAFTDQANSYIRPIINFLVKNGAPLLANVYPYFSYTSNKQGISLDYALFRQQGNNAVGYNNLFDAMLDSVYAALEKVGGSNVKIVVSESGWPSKGGDSASFDNAATYYSNLINHIKNGTPMRPGAAIETYLFAMFDENQKTGLATEQHYGLFNPDKSPKYHVAQSGIELDTTRNITVILNMK*