AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021793.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021793.01.T01 MTR_4g076490 89.969 319 32 0 1 319 103 421 0.0 590
MsG0480021793.01.T01 MTR_4g076470 82.333 300 52 1 1 300 29 327 0.0 507
MsG0480021793.01.T01 MTR_4g076440 80.000 300 59 1 1 300 29 327 0.0 502
MsG0480021793.01.T01 MTR_4g076430 81.000 300 56 1 1 300 29 327 1.51e-178 496
MsG0480021793.01.T01 MTR_4g076255 72.148 298 82 1 3 300 33 329 3.38e-158 445
MsG0480021793.01.T01 MTR_4g076500 62.791 301 107 4 2 297 85 385 2.96e-126 366
MsG0480021793.01.T01 MTR_1g052430 56.958 309 121 4 2 301 39 344 2.16e-119 350
MsG0480021793.01.T01 MTR_4g076570 56.051 314 130 4 2 308 29 341 2.85e-118 345
MsG0480021793.01.T01 MTR_2g034480 52.733 311 137 5 1 303 33 341 1.73e-102 305
MsG0480021793.01.T01 MTR_2g034470 50.153 327 152 6 1 319 1 324 9.68e-102 302
MsG0480021793.01.T01 MTR_2g034470 50.153 327 152 6 1 319 36 359 1.81e-101 302
MsG0480021793.01.T01 MTR_2g034440 51.307 306 138 6 1 297 28 331 1.73e-98 294
MsG0480021793.01.T01 MTR_7g081350 44.040 302 159 7 3 297 35 333 6.83e-81 249
MsG0480021793.01.T01 MTR_3g080410 42.284 324 174 7 2 312 33 356 8.09e-76 237
MsG0480021793.01.T01 MTR_5g085580 42.628 312 164 7 2 299 32 342 2.17e-75 239
MsG0480021793.01.T01 MTR_7g081370 42.140 299 156 7 3 297 36 321 8.99e-73 228
MsG0480021793.01.T01 MTR_4g052970 39.683 315 176 6 2 303 36 349 6.66e-72 227
MsG0480021793.01.T01 MTR_4g069940 38.782 312 178 5 2 300 36 347 1.68e-70 226
MsG0480021793.01.T01 MTR_4g084480 39.683 315 178 7 2 304 32 346 2.87e-70 223
MsG0480021793.01.T01 MTR_4g084480 39.683 315 178 7 2 304 39 353 3.18e-70 223
MsG0480021793.01.T01 MTR_4g084480 39.683 315 178 7 2 304 39 353 3.22e-70 223
MsG0480021793.01.T01 MTR_2g086530 37.942 311 179 5 2 300 15 323 4.11e-70 222
MsG0480021793.01.T01 MTR_2g086530 37.942 311 179 5 2 300 36 344 6.20e-70 223
MsG0480021793.01.T01 MTR_1g077990 39.423 312 178 6 2 302 38 349 8.62e-70 223
MsG0480021793.01.T01 MTR_4g010200 37.224 317 186 5 2 305 40 356 2.53e-67 216
MsG0480021793.01.T01 MTR_1g056640 38.720 328 184 8 2 315 45 369 2.44e-66 216
MsG0480021793.01.T01 MTR_4g088155 35.168 327 192 7 1 308 29 354 7.74e-66 214
MsG0480021793.01.T01 MTR_3g116510 36.570 309 183 5 4 299 37 345 1.21e-65 213
MsG0480021793.01.T01 MTR_6g088885 34.185 313 194 5 3 303 35 347 2.02e-64 211
MsG0480021793.01.T01 MTR_3g065460 37.143 315 183 5 4 303 33 347 2.02e-64 211
MsG0480021793.01.T01 MTR_1g031900 37.261 314 182 5 5 303 34 347 2.83e-64 211
MsG0480021793.01.T01 MTR_1g007810 37.299 311 175 8 2 297 23 328 4.35e-64 206
MsG0480021793.01.T01 MTR_1g031900 37.261 314 182 5 5 303 34 347 6.63e-64 210
MsG0480021793.01.T01 MTR_1g022160 36.825 315 185 6 2 302 31 345 8.90e-64 207
MsG0480021793.01.T01 MTR_5g078200 37.651 332 186 8 4 315 32 362 1.68e-63 212
MsG0480021793.01.T01 MTR_4g114850 36.076 316 189 6 2 304 33 348 1.75e-63 205
MsG0480021793.01.T01 MTR_4g114850 36.076 316 189 6 2 304 33 348 1.91e-63 205
MsG0480021793.01.T01 MTR_5g078200 37.879 330 184 8 6 315 1 329 2.26e-63 211
MsG0480021793.01.T01 MTR_4g114850 36.076 316 189 6 2 304 33 348 2.42e-63 205
MsG0480021793.01.T01 MTR_2g019345 38.585 311 178 6 2 300 31 340 4.85e-63 205
MsG0480021793.01.T01 MTR_1g022160 36.825 315 185 6 2 302 31 345 5.53e-63 207
MsG0480021793.01.T01 MTR_1g022160 36.825 315 185 6 2 302 31 345 5.64e-63 207
MsG0480021793.01.T01 MTR_1g022160 36.825 315 185 6 2 302 31 345 6.64e-63 207
MsG0480021793.01.T01 MTR_8g102340 36.567 268 156 5 60 313 60 327 9.51e-61 201
MsG0480021793.01.T01 MTR_8g012400 33.856 319 193 7 2 303 36 353 3.66e-60 199
MsG0480021793.01.T01 MTR_7g053500 33.750 320 192 8 2 303 33 350 9.62e-60 198
MsG0480021793.01.T01 MTR_3g083580 34.667 300 181 5 5 289 36 335 6.35e-57 191
MsG0480021793.01.T01 MTR_7g026340 33.945 327 195 10 2 315 30 348 1.63e-56 189
MsG0480021793.01.T01 MTR_4g132280 33.968 315 193 5 5 304 55 369 6.98e-56 189
MsG0480021793.01.T01 MTR_8g012400 35.361 263 153 6 57 303 12 273 6.81e-52 176
MsG0480021793.01.T01 MTR_7g085390 35.256 312 180 11 10 303 41 348 8.60e-52 177
MsG0480021793.01.T01 MTR_7g053500 35.606 264 151 7 57 303 12 273 1.86e-51 174
MsG0480021793.01.T01 MTR_6g032820 35.922 309 176 10 12 300 43 349 3.27e-51 175
MsG0480021793.01.T01 MTR_8g085720 31.746 315 200 5 4 303 35 349 4.80e-50 173
MsG0480021793.01.T01 MTR_4g083500 34.505 313 188 9 2 300 51 360 1.52e-49 173
MsG0480021793.01.T01 MTR_8g092070 33.550 307 187 10 12 303 51 355 3.78e-49 172
MsG0480021793.01.T01 MTR_2g027560 34.268 321 188 11 12 312 45 362 5.26e-48 168
MsG0480021793.01.T01 MTR_4g112025 35.135 333 178 13 1 307 38 358 1.40e-47 167
MsG0480021793.01.T01 MTR_3g116510 37.736 212 122 3 99 300 1 212 1.49e-47 162
MsG0480021793.01.T01 MTR_3g095050 33.981 309 181 12 12 303 51 353 1.24e-45 161
MsG0480021793.01.T01 MTR_1g053320 33.125 320 195 11 1 303 37 354 1.79e-45 161
MsG0480021793.01.T01 MTR_4g124440 33.227 313 191 11 6 303 40 349 2.48e-44 158
MsG0480021793.01.T01 MTR_1g010120 33.115 305 183 10 12 299 43 343 1.41e-41 150
MsG0480021793.01.T01 MTR_3g031770 33.882 304 182 11 11 299 43 342 3.85e-41 149
MsG0480021793.01.T01 MTR_3g108600 33.333 312 168 12 5 299 41 329 3.42e-40 146
MsG0480021793.01.T01 MTR_4g036775 57.143 98 42 0 204 301 80 177 1.87e-34 125
MsG0480021793.01.T01 MTR_4g036775 53.333 60 28 0 2 61 29 88 5.36e-13 67.8
MsG0480021793.01.T01 MTR_4g036680 57.143 98 42 0 204 301 80 177 2.00e-34 125
MsG0480021793.01.T01 MTR_4g036680 51.667 60 29 0 2 61 29 88 5.01e-12 64.7
MsG0480021793.01.T01 MTR_4g134280 33.468 248 145 10 81 312 2 245 1.63e-33 127
MsG0480021793.01.T01 MTR_5g081720 29.897 291 185 10 21 297 63 348 3.88e-33 126
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021793.01.T01 AT4G16260 49.675 308 147 4 1 302 1 306 8.70e-105 309
MsG0480021793.01.T01 AT4G16260 49.675 308 147 4 1 302 30 335 1.54e-104 310
MsG0480021793.01.T01 AT3G57270 50.165 303 145 3 3 299 35 337 4.37e-100 298
MsG0480021793.01.T01 AT3G57260 51.316 304 138 6 1 299 38 336 9.87e-97 290
MsG0480021793.01.T01 AT3G57260 51.316 304 138 6 1 299 54 352 1.25e-96 290
MsG0480021793.01.T01 AT3G57240 50.658 304 135 6 3 299 43 338 6.54e-96 287
MsG0480021793.01.T01 AT5G20340 41.748 309 166 9 1 299 49 353 1.85e-74 233
MsG0480021793.01.T01 AT2G05790 40.836 311 171 6 2 299 32 342 1.30e-72 232
MsG0480021793.01.T01 AT4G26830 40.645 310 172 5 2 299 32 341 2.94e-72 230
MsG0480021793.01.T01 AT4G26830 40.645 310 172 5 2 299 36 345 3.62e-72 230
MsG0480021793.01.T01 AT5G20330 41.100 309 168 9 1 299 40 344 8.96e-72 226
MsG0480021793.01.T01 AT2G01630 39.809 314 174 5 5 303 33 346 9.84e-72 227
MsG0480021793.01.T01 AT2G01630 39.809 314 174 5 5 303 33 346 4.69e-71 227
MsG0480021793.01.T01 AT5G56590 37.143 315 184 5 3 303 34 348 1.07e-70 228
MsG0480021793.01.T01 AT5G55180 39.557 316 176 8 2 303 34 348 1.08e-70 226
MsG0480021793.01.T01 AT5G55180 39.557 316 176 8 2 303 34 348 1.15e-70 226
MsG0480021793.01.T01 AT5G20390 39.482 309 173 9 1 299 39 343 2.75e-70 222
MsG0480021793.01.T01 AT2G01630 39.809 314 174 5 5 303 33 346 3.17e-70 226
MsG0480021793.01.T01 AT1G32860 38.019 313 180 6 2 300 36 348 4.89e-70 224
MsG0480021793.01.T01 AT2G16230 37.898 314 180 6 1 300 31 343 3.32e-69 224
MsG0480021793.01.T01 AT2G27500 35.542 332 198 7 2 317 36 367 8.39e-69 219
MsG0480021793.01.T01 AT2G27500 35.928 334 197 8 2 318 36 369 1.52e-68 219
MsG0480021793.01.T01 AT1G33220 40.803 299 164 8 1 289 40 335 1.24e-67 215
MsG0480021793.01.T01 AT5G42100 39.550 311 173 6 2 297 34 344 1.27e-67 218
MsG0480021793.01.T01 AT4G18340 38.154 325 189 6 2 314 41 365 1.50e-67 216
MsG0480021793.01.T01 AT5G42100 39.172 314 176 6 2 300 34 347 1.90e-67 217
MsG0480021793.01.T01 AT4G18340 38.154 325 189 6 2 314 41 365 2.11e-67 216
MsG0480021793.01.T01 AT1G77790 38.436 307 176 7 3 299 38 341 1.84e-66 212
MsG0480021793.01.T01 AT5G42720 38.658 313 178 7 1 299 32 344 6.14e-66 214
MsG0480021793.01.T01 AT3G15800 36.792 318 187 6 2 305 50 367 1.89e-65 211
MsG0480021793.01.T01 AT4G34480 38.264 311 179 5 2 299 33 343 1.14e-63 205
MsG0480021793.01.T01 AT1G66250 38.312 308 174 6 5 297 42 348 2.21e-62 206
MsG0480021793.01.T01 AT2G26600 33.333 324 203 7 2 312 41 364 3.11e-62 202
MsG0480021793.01.T01 AT4G34480 38.264 311 179 5 2 299 33 343 3.84e-62 206
MsG0480021793.01.T01 AT1G30080 36.656 311 185 6 2 300 43 353 1.53e-61 201
MsG0480021793.01.T01 AT3G07320 35.110 319 189 8 2 303 32 349 1.61e-61 202
MsG0480021793.01.T01 AT1G30080 36.656 311 185 6 2 300 43 353 2.98e-61 201
MsG0480021793.01.T01 AT5G24318 34.395 314 186 8 2 300 37 345 4.20e-61 199
MsG0480021793.01.T01 AT5G20560 35.667 300 181 7 1 291 40 336 7.20e-61 197
MsG0480021793.01.T01 AT1G77780 36.184 304 180 9 3 297 32 330 2.38e-60 197
MsG0480021793.01.T01 AT1G11820 34.069 317 192 6 4 303 53 369 4.10e-60 197
MsG0480021793.01.T01 AT5G24318 34.395 314 186 8 2 300 37 345 4.33e-60 199
MsG0480021793.01.T01 AT5G24318 34.395 314 186 8 2 300 37 345 6.98e-60 198
MsG0480021793.01.T01 AT2G27500 37.050 278 159 7 57 318 14 291 7.15e-60 194
MsG0480021793.01.T01 AT1G11820 34.139 331 199 7 4 315 53 383 1.02e-59 198
MsG0480021793.01.T01 AT1G11820 34.139 331 199 7 4 315 53 383 2.77e-59 198
MsG0480021793.01.T01 AT4G14080 35.331 317 188 7 3 303 50 365 5.01e-59 197
MsG0480021793.01.T01 AT3G61810 34.516 310 183 5 3 297 63 367 5.84e-59 194
MsG0480021793.01.T01 AT4G29360 36.789 299 177 6 3 289 35 333 6.09e-58 195
MsG0480021793.01.T01 AT4G29360 36.789 299 177 6 3 289 45 343 7.69e-58 195
MsG0480021793.01.T01 AT3G23770 35.202 321 188 8 2 303 44 363 5.65e-57 191
MsG0480021793.01.T01 AT3G13560 32.792 308 192 5 5 297 36 343 2.74e-53 182
MsG0480021793.01.T01 AT3G13560 32.792 308 192 5 5 297 36 343 2.74e-53 182
MsG0480021793.01.T01 AT3G13560 32.792 308 192 5 5 297 36 343 2.74e-53 182
MsG0480021793.01.T01 AT3G13560 32.792 308 192 5 5 297 36 343 2.74e-53 182
MsG0480021793.01.T01 AT3G13560 32.792 308 192 5 5 297 36 343 2.74e-53 182
MsG0480021793.01.T01 AT2G26600 33.209 268 167 6 57 312 3 270 4.30e-52 174
MsG0480021793.01.T01 AT5G18220 33.754 317 192 9 1 303 37 349 1.86e-49 172
MsG0480021793.01.T01 AT3G46570 33.962 318 183 11 2 300 34 343 1.70e-48 166
MsG0480021793.01.T01 AT1G64760 34.169 319 190 12 1 303 32 346 3.91e-48 168
MsG0480021793.01.T01 AT1G64760 34.169 319 190 12 1 303 32 346 3.91e-48 168
MsG0480021793.01.T01 AT2G19440 33.542 319 192 12 1 303 28 342 1.50e-47 166
MsG0480021793.01.T01 AT2G19440 33.542 319 192 12 1 303 28 342 1.50e-47 166
MsG0480021793.01.T01 AT4G34480 41.126 231 124 4 82 300 3 233 2.22e-47 164
MsG0480021793.01.T01 AT3G55430 33.762 311 185 11 5 300 38 342 3.97e-47 165
MsG0480021793.01.T01 AT3G24330 32.710 321 194 11 1 303 46 362 1.29e-45 162
MsG0480021793.01.T01 AT4G31140 33.553 304 185 10 12 300 44 345 1.39e-45 161
MsG0480021793.01.T01 AT5G58090 34.323 303 178 10 12 297 39 337 1.50e-45 161
MsG0480021793.01.T01 AT2G39640 31.699 306 194 7 5 300 39 339 3.35e-45 161
MsG0480021793.01.T01 AT3G04010 33.333 321 190 11 1 303 40 354 5.46e-45 160
MsG0480021793.01.T01 AT5G58480 34.516 310 182 11 7 300 40 344 1.60e-44 158
MsG0480021793.01.T01 AT5G24318 35.217 230 129 7 102 319 1 222 6.48e-40 142
MsG0480021793.01.T01 AT4G17180 30.464 302 190 10 12 299 42 337 2.15e-39 144
MsG0480021793.01.T01 AT5G64790 30.744 309 193 10 12 303 45 349 7.80e-39 143
MsG0480021793.01.T01 AT5G20870 30.476 315 187 13 12 300 47 355 2.55e-35 134
MsG0480021793.01.T01 AT3G55780 28.803 309 194 12 8 297 48 349 1.99e-33 127

Find 76 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGATTTGGCCTTGTAAAT+TGG 0.228491 4:-64341400 None:intergenic
TGTCATCTTAAATATGAAAT+AGG 0.232338 4:+64342004 MsG0480021793.01.T01:CDS
ATGCCAGTGAAGCCACAAAT+TGG 0.263953 4:+64341238 MsG0480021793.01.T01:CDS
ACTCAATGCTGGAACCTCTA+AGG 0.278148 4:-64341173 None:intergenic
TCTACAACTTCTTGCTTTGA+TGG 0.282682 4:-64341084 None:intergenic
TTCACTAGGAAGTTAATTAT+TGG 0.314710 4:-64341513 None:intergenic
GACTGTGCCACATGGTATTT+AGG 0.330651 4:-64341948 None:intergenic
GCAATTGACATGACTTTGAT+TGG 0.370164 4:+64341432 MsG0480021793.01.T01:CDS
GGCGTTTGTTATGGAATGAT+TGG 0.376346 4:+64341051 None:intergenic
TTGCTGTTGAGACCTTGATT+TGG 0.384562 4:-64341413 None:intergenic
GACCCAATTTGTGGCTTCAC+TGG 0.390549 4:-64341241 None:intergenic
GCTTGTAGGACTTCTTCATC+TGG 0.416204 4:-64341150 None:intergenic
ATAGCAGCACCGGGCCTCAT+AGG 0.417015 4:-64341834 None:intergenic
TGGAACCTCTAAGGGCTTGT+AGG 0.422796 4:-64341164 None:intergenic
GAAGTCCTACAAGCCCTTAG+AGG 0.427617 4:+64341159 MsG0480021793.01.T01:CDS
GTCGGTGAAAGCTCCATCAT+TGG 0.432640 4:-64341469 None:intergenic
GGCATTTGTAAGAGAAGAAA+GGG 0.443087 4:-64341220 None:intergenic
GTCGTATATATGAATTTGCT+TGG 0.444902 4:-64341491 None:intergenic
TATATATGAATTTGCTTGGT+CGG 0.459241 4:-64341487 None:intergenic
TGCCAGTGAAGCCACAAATT+GGG 0.459796 4:+64341239 MsG0480021793.01.T01:CDS
GTAGATTTATATAAATCAAA+AGG 0.468589 4:+64341102 MsG0480021793.01.T01:CDS
ATATTCACATCTTGAGCATA+GGG 0.477409 4:-64341279 None:intergenic
TGGTATTTAGGTGATTTGTC+AGG 0.481833 4:-64341936 None:intergenic
TGGCATTTGTAAGAGAAGAA+AGG 0.491310 4:-64341221 None:intergenic
CTCAATGCTGGAACCTCTAA+GGG 0.492908 4:-64341172 None:intergenic
GATATTCACATCTTGAGCAT+AGG 0.494146 4:-64341280 None:intergenic
ATTTCATCAGCCAATTTACA+AGG 0.495424 4:+64341390 MsG0480021793.01.T01:CDS
TCGGTGAAAGCTCCATCATT+GGG 0.497932 4:-64341468 None:intergenic
GTAAGTTTCAATAGCAGCAC+CGG 0.508709 4:-64341844 None:intergenic
CTAGCAACTGAACAACACTA+TGG 0.509757 4:+64341903 MsG0480021793.01.T01:CDS
TTGATCAATCACATCAAGAA+TGG 0.511422 4:+64341807 MsG0480021793.01.T01:CDS
TTTGATGAAAATCAGAAGAC+TGG 0.512681 4:+64341879 MsG0480021793.01.T01:CDS
AATATCAAGTACATCACTGT+TGG 0.518524 4:+64341297 MsG0480021793.01.T01:CDS
AAGTCATATCCACCCAATGA+TGG 0.518679 4:+64341456 MsG0480021793.01.T01:CDS
TACGCTGCTCTCGAGAAAGT+TGG 0.518967 4:+64341687 MsG0480021793.01.T01:CDS
CAACAAGGAAACAATGCAGT+TGG 0.521019 4:+64341627 MsG0480021793.01.T01:CDS
CTTGTAGGACTTCTTCATCT+GGG 0.522874 4:-64341149 None:intergenic
TTATATAAATCAAAAGGCAT+TGG 0.524198 4:+64341108 MsG0480021793.01.T01:CDS
GAGAGTGGATGGCCGTCTAA+AGG 0.525547 4:+64341738 MsG0480021793.01.T01:CDS
GTGGTGCTCCGTTCTTCACT+AGG 0.526030 4:-64341527 None:intergenic
GTTGATCCTTGCTGTGGCCA+TGG 0.530355 4:+64341194 MsG0480021793.01.T01:CDS
AGCAAGGATCAACTCAATGC+TGG 0.530815 4:-64341184 None:intergenic
TAAATACCATGTGGCACAGT+CGG 0.536931 4:+64341950 MsG0480021793.01.T01:CDS
AACAAGGAAACAATGCAGTT+GGG 0.544475 4:+64341628 MsG0480021793.01.T01:CDS
TCAAGAATGGCACTCCTATG+AGG 0.547579 4:+64341820 MsG0480021793.01.T01:CDS
AAGAGAAGAAAGGGTTTCCA+TGG 0.551742 4:-64341211 None:intergenic
TGTGGCAGCATTGTTAATTG+AGG 0.555373 4:-64341772 None:intergenic
AAATACCATGTGGCACAGTC+GGG 0.556287 4:+64341951 MsG0480021793.01.T01:CDS
GCTGCTCTCGAGAAAGTTGG+AGG 0.568267 4:+64341690 MsG0480021793.01.T01:CDS
GGCACAGTCGGGTATCGAGT+TGG 0.574353 4:+64341962 MsG0480021793.01.T01:CDS
TAGTTGTGTCCGAGAGTGGA+TGG 0.576028 4:+64341727 MsG0480021793.01.T01:CDS
TTTAGACGGCCATCCACTCT+CGG 0.576371 4:-64341736 None:intergenic
AATGGCACTCCTATGAGGCC+CGG 0.577050 4:+64341825 MsG0480021793.01.T01:CDS
CAATTTACAAGGCCAAATCA+AGG 0.582492 4:+64341401 MsG0480021793.01.T01:CDS
AAGATAGTTGTGTCCGAGAG+TGG 0.586296 4:+64341723 MsG0480021793.01.T01:CDS
GATCAAATTAGAATAATATG+TGG 0.597633 4:-64341790 None:intergenic
GCACCATCTCCACCTTTAGA+CGG 0.600735 4:-64341750 None:intergenic
GACTGAGCCTCATTGTCAAG+AGG 0.606841 4:-64341333 None:intergenic
GGATACACATTGGCAAGAAG+TGG 0.610222 4:-64341546 None:intergenic
AATTCGCCCTCTTGACAATG+AGG 0.612865 4:+64341326 MsG0480021793.01.T01:CDS
AGTGGATGGCCGTCTAAAGG+TGG 0.613040 4:+64341741 MsG0480021793.01.T01:CDS
ATTAACTTCCTAGTGAAGAA+CGG 0.614709 4:+64341519 MsG0480021793.01.T01:CDS
CGATACCCGACTGTGCCACA+TGG 0.618586 4:-64341956 None:intergenic
TGGCCGTCTAAAGGTGGAGA+TGG 0.619833 4:+64341747 MsG0480021793.01.T01:CDS
CATTGAGTTGATCCTTGCTG+TGG 0.621915 4:+64341188 MsG0480021793.01.T01:CDS
TTATCGATATAAGCGAAGTA+CGG 0.622916 4:-64341567 None:intergenic
GGGTTTCCATGGCCACAGCA+AGG 0.633168 4:-64341200 None:intergenic
GGACACAACTATCTTCACAT+TGG 0.633314 4:-64341715 None:intergenic
TAAGTTTCAATAGCAGCACC+GGG 0.642794 4:-64341843 None:intergenic
AATCTAACATGGCATCAAAG+AGG 0.643071 4:-64341659 None:intergenic
ACTGAGCCTCATTGTCAAGA+GGG 0.643311 4:-64341332 None:intergenic
AGCGAAGTACGGATACACAT+TGG 0.661063 4:-64341556 None:intergenic
GTGAAAGCTCCATCATTGGG+TGG 0.661888 4:-64341465 None:intergenic
AGCGTATACTGAATCTAACA+TGG 0.678826 4:-64341670 None:intergenic
CAAATCACCTAAATACCATG+TGG 0.698839 4:+64341941 MsG0480021793.01.T01:CDS
GTATCGAGTTGGATACAACA+AGG 0.731881 4:+64341973 MsG0480021793.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAGATTTATATAAATCAAA+AGG + Chr4:64341102-64341121 MsG0480021793.01.T01:CDS 15.0%
!! GATCAAATTAGAATAATATG+TGG - Chr4:64341793-64341812 None:intergenic 20.0%
!!! TTATATAAATCAAAAGGCAT+TGG + Chr4:64341108-64341127 MsG0480021793.01.T01:CDS 20.0%
! TTCACTAGGAAGTTAATTAT+TGG - Chr4:64341516-64341535 None:intergenic 25.0%
!! TATATATGAATTTGCTTGGT+CGG - Chr4:64341490-64341509 None:intergenic 25.0%
!! TGCATTTTGAATGTTTTTCA+AGG - Chr4:64341370-64341389 None:intergenic 25.0%
AATATCAAGTACATCACTGT+TGG + Chr4:64341297-64341316 MsG0480021793.01.T01:CDS 30.0%
ATATTCACATCTTGAGCATA+GGG - Chr4:64341282-64341301 None:intergenic 30.0%
ATTAACTTCCTAGTGAAGAA+CGG + Chr4:64341519-64341538 MsG0480021793.01.T01:CDS 30.0%
ATTTCATCAGCCAATTTACA+AGG + Chr4:64341390-64341409 MsG0480021793.01.T01:CDS 30.0%
TTATCGATATAAGCGAAGTA+CGG - Chr4:64341570-64341589 None:intergenic 30.0%
TTGATCAATCACATCAAGAA+TGG + Chr4:64341807-64341826 MsG0480021793.01.T01:CDS 30.0%
TTTGATGAAAATCAGAAGAC+TGG + Chr4:64341879-64341898 MsG0480021793.01.T01:CDS 30.0%
! CTTCTGATTTTCATCAAACA+TGG - Chr4:64341877-64341896 None:intergenic 30.0%
! GTCGTATATATGAATTTGCT+TGG - Chr4:64341494-64341513 None:intergenic 30.0%
! TATGCTCTTTTTAGACAACA+AGG + Chr4:64341612-64341631 MsG0480021793.01.T01:CDS 30.0%
!!! ATATTTTTGGACCCAATTTG+TGG - Chr4:64341253-64341272 None:intergenic 30.0%
!!! TTTGAATGTTTTTCAAGGCA+GGG - Chr4:64341365-64341384 None:intergenic 30.0%
!!! TTTTGAATGTTTTTCAAGGC+AGG - Chr4:64341366-64341385 None:intergenic 30.0%
AACAAGGAAACAATGCAGTT+GGG + Chr4:64341628-64341647 MsG0480021793.01.T01:CDS 35.0%
AATCTAACATGGCATCAAAG+AGG - Chr4:64341662-64341681 None:intergenic 35.0%
AGCGTATACTGAATCTAACA+TGG - Chr4:64341673-64341692 None:intergenic 35.0%
CAAATCACCTAAATACCATG+TGG + Chr4:64341941-64341960 MsG0480021793.01.T01:CDS 35.0%
CAATTTACAAGGCCAAATCA+AGG + Chr4:64341401-64341420 MsG0480021793.01.T01:CDS 35.0%
GATATTCACATCTTGAGCAT+AGG - Chr4:64341283-64341302 None:intergenic 35.0%
GCAATTGACATGACTTTGAT+TGG + Chr4:64341432-64341451 MsG0480021793.01.T01:CDS 35.0%
GGCATTTGTAAGAGAAGAAA+GGG - Chr4:64341223-64341242 None:intergenic 35.0%
TCTACAACTTCTTGCTTTGA+TGG - Chr4:64341087-64341106 None:intergenic 35.0%
TGGCATTTGTAAGAGAAGAA+AGG - Chr4:64341224-64341243 None:intergenic 35.0%
! TGGTATTTAGGTGATTTGTC+AGG - Chr4:64341939-64341958 None:intergenic 35.0%
!! CATAGGGTGTCACATATTTT+TGG - Chr4:64341266-64341285 None:intergenic 35.0%
!! CTTGATTTGGCCTTGTAAAT+TGG - Chr4:64341403-64341422 None:intergenic 35.0%
AAGAGAAGAAAGGGTTTCCA+TGG - Chr4:64341214-64341233 None:intergenic 40.0%
AAGTCATATCCACCCAATGA+TGG + Chr4:64341456-64341475 MsG0480021793.01.T01:CDS 40.0%
CAACAAGGAAACAATGCAGT+TGG + Chr4:64341627-64341646 MsG0480021793.01.T01:CDS 40.0%
CTAGCAACTGAACAACACTA+TGG + Chr4:64341903-64341922 MsG0480021793.01.T01:CDS 40.0%
CTTGTAGGACTTCTTCATCT+GGG - Chr4:64341152-64341171 None:intergenic 40.0%
GGACACAACTATCTTCACAT+TGG - Chr4:64341718-64341737 None:intergenic 40.0%
GTAAGTTTCAATAGCAGCAC+CGG - Chr4:64341847-64341866 None:intergenic 40.0%
TAAATACCATGTGGCACAGT+CGG + Chr4:64341950-64341969 MsG0480021793.01.T01:CDS 40.0%
TAAGTTTCAATAGCAGCACC+GGG - Chr4:64341846-64341865 None:intergenic 40.0%
TGTGGCAGCATTGTTAATTG+AGG - Chr4:64341775-64341794 None:intergenic 40.0%
! TTGCTGTTGAGACCTTGATT+TGG - Chr4:64341416-64341435 None:intergenic 40.0%
!! GTATCGAGTTGGATACAACA+AGG + Chr4:64341973-64341992 MsG0480021793.01.T01:CDS 40.0%
AAATACCATGTGGCACAGTC+GGG + Chr4:64341951-64341970 MsG0480021793.01.T01:CDS 45.0%
AAGATAGTTGTGTCCGAGAG+TGG + Chr4:64341723-64341742 MsG0480021793.01.T01:CDS 45.0%
AATTCGCCCTCTTGACAATG+AGG + Chr4:64341326-64341345 MsG0480021793.01.T01:CDS 45.0%
ACTCAATGCTGGAACCTCTA+AGG - Chr4:64341176-64341195 None:intergenic 45.0%
ACTGAGCCTCATTGTCAAGA+GGG - Chr4:64341335-64341354 None:intergenic 45.0%
AGCAAGGATCAACTCAATGC+TGG - Chr4:64341187-64341206 None:intergenic 45.0%
AGCGAAGTACGGATACACAT+TGG - Chr4:64341559-64341578 None:intergenic 45.0%
ATGCCAGTGAAGCCACAAAT+TGG + Chr4:64341238-64341257 MsG0480021793.01.T01:CDS 45.0%
CTCAATGCTGGAACCTCTAA+GGG - Chr4:64341175-64341194 None:intergenic 45.0%
GACTGTGCCACATGGTATTT+AGG - Chr4:64341951-64341970 None:intergenic 45.0%
GCTTGTAGGACTTCTTCATC+TGG - Chr4:64341153-64341172 None:intergenic 45.0%
GGATACACATTGGCAAGAAG+TGG - Chr4:64341549-64341568 None:intergenic 45.0%
TCAAGAATGGCACTCCTATG+AGG + Chr4:64341820-64341839 MsG0480021793.01.T01:CDS 45.0%
TCGGTGAAAGCTCCATCATT+GGG - Chr4:64341471-64341490 None:intergenic 45.0%
TGCCAGTGAAGCCACAAATT+GGG + Chr4:64341239-64341258 MsG0480021793.01.T01:CDS 45.0%
!! CATTGAGTTGATCCTTGCTG+TGG + Chr4:64341188-64341207 MsG0480021793.01.T01:CDS 45.0%
!!! GTTTTTCAAGGCAGGGAGAA+TGG - Chr4:64341358-64341377 None:intergenic 45.0%
GAAGTCCTACAAGCCCTTAG+AGG + Chr4:64341159-64341178 MsG0480021793.01.T01:CDS 50.0%
GACCCAATTTGTGGCTTCAC+TGG - Chr4:64341244-64341263 None:intergenic 50.0%
GACTGAGCCTCATTGTCAAG+AGG - Chr4:64341336-64341355 None:intergenic 50.0%
GCACCATCTCCACCTTTAGA+CGG - Chr4:64341753-64341772 None:intergenic 50.0%
GTCGGTGAAAGCTCCATCAT+TGG - Chr4:64341472-64341491 None:intergenic 50.0%
GTGAAAGCTCCATCATTGGG+TGG - Chr4:64341468-64341487 None:intergenic 50.0%
TACGCTGCTCTCGAGAAAGT+TGG + Chr4:64341687-64341706 MsG0480021793.01.T01:CDS 50.0%
TAGTTGTGTCCGAGAGTGGA+TGG + Chr4:64341727-64341746 MsG0480021793.01.T01:CDS 50.0%
TTTAGACGGCCATCCACTCT+CGG - Chr4:64341739-64341758 None:intergenic 50.0%
! TGGAACCTCTAAGGGCTTGT+AGG - Chr4:64341167-64341186 None:intergenic 50.0%
AATGGCACTCCTATGAGGCC+CGG + Chr4:64341825-64341844 MsG0480021793.01.T01:CDS 55.0%
AGTGGATGGCCGTCTAAAGG+TGG + Chr4:64341741-64341760 MsG0480021793.01.T01:CDS 55.0%
GAGAGTGGATGGCCGTCTAA+AGG + Chr4:64341738-64341757 MsG0480021793.01.T01:CDS 55.0%
GCTGCTCTCGAGAAAGTTGG+AGG + Chr4:64341690-64341709 MsG0480021793.01.T01:CDS 55.0%
GTGGTGCTCCGTTCTTCACT+AGG - Chr4:64341530-64341549 None:intergenic 55.0%
TGGCCGTCTAAAGGTGGAGA+TGG + Chr4:64341747-64341766 MsG0480021793.01.T01:CDS 55.0%
! GTTGATCCTTGCTGTGGCCA+TGG + Chr4:64341194-64341213 MsG0480021793.01.T01:CDS 55.0%
CGATACCCGACTGTGCCACA+TGG - Chr4:64341959-64341978 None:intergenic 60.0%
GGGTTTCCATGGCCACAGCA+AGG - Chr4:64341203-64341222 None:intergenic 60.0%
! ATAGCAGCACCGGGCCTCAT+AGG - Chr4:64341837-64341856 None:intergenic 60.0%
!! GGCACAGTCGGGTATCGAGT+TGG + Chr4:64341962-64341981 MsG0480021793.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 64341066 64342025 64341066 ID=MsG0480021793.01;Name=MsG0480021793.01
Chr4 mRNA 64341066 64342025 64341066 ID=MsG0480021793.01.T01;Parent=MsG0480021793.01;Name=MsG0480021793.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|319
Chr4 exon 64341066 64342025 64341066 ID=MsG0480021793.01.T01:exon:7764;Parent=MsG0480021793.01.T01
Chr4 CDS 64341066 64342025 64341066 ID=MsG0480021793.01.T01:cds;Parent=MsG0480021793.01.T01
Gene Sequence

>MsG0480021793.01.T01

ATGATTGGCAATAATCTACCATCAAAGCAAGAAGTTGTAGATTTATATAAATCAAAAGGCATTGGCAAAATGCGTATATACTACCCAGATGAAGAAGTCCTACAAGCCCTTAGAGGTTCCAGCATTGAGTTGATCCTTGCTGTGGCCATGGAAACCCTTTCTTCTCTTACAAATGCCAGTGAAGCCACAAATTGGGTCCAAAAATATGTGACACCCTATGCTCAAGATGTGAATATCAAGTACATCACTGTTGGAAATGAAATTCGCCCTCTTGACAATGAGGCTCAGTCCATTCTCCCTGCCTTGAAAAACATTCAAAATGCAATTTCATCAGCCAATTTACAAGGCCAAATCAAGGTCTCAACAGCAATTGACATGACTTTGATTGGTAAGTCATATCCACCCAATGATGGAGCTTTCACCGACCAAGCAAATTCATATATACGACCAATAATTAACTTCCTAGTGAAGAACGGAGCACCACTTCTTGCCAATGTGTATCCGTACTTCGCTTATATCGATAATAAGCAAAGCATTTCTCTTGACTATGCTCTTTTTAGACAACAAGGAAACAATGCAGTTGGGTACAGCAACCTCTTTGATGCCATGTTAGATTCAGTATACGCTGCTCTCGAGAAAGTTGGAGGTTCCAATGTGAAGATAGTTGTGTCCGAGAGTGGATGGCCGTCTAAAGGTGGAGATGGTGCCTCAATTAACAATGCTGCCACATATTATTCTAATTTGATCAATCACATCAAGAATGGCACTCCTATGAGGCCCGGTGCTGCTATTGAAACTTACTTGTTTGCCATGTTTGATGAAAATCAGAAGACTGGTCTAGCAACTGAACAACACTATGGTCTCTTTAATCCTGACAAATCACCTAAATACCATGTGGCACAGTCGGGTATCGAGTTGGATACAACAAGGAACATTACTGTCATCTTAAATATGAAATAG
Protein sequence

>MsG0480021793.01.T01

MIGNNLPSKQEVVDLYKSKGIGKMRIYYPDEEVLQALRGSSIELILAVAMETLSSLTNASEATNWVQKYVTPYAQDVNIKYITVGNEIRPLDNEAQSILPALKNIQNAISSANLQGQIKVSTAIDMTLIGKSYPPNDGAFTDQANSYIRPIINFLVKNGAPLLANVYPYFAYIDNKQSISLDYALFRQQGNNAVGYSNLFDAMLDSVYAALEKVGGSNVKIVVSESGWPSKGGDGASINNAATYYSNLINHIKNGTPMRPGAAIETYLFAMFDENQKTGLATEQHYGLFNPDKSPKYHVAQSGIELDTTRNITVILNMK*