AlfalfaGEDB | MsG0480021916.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021916.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021916.01.T01	XP_004505629.1	72.308	65	18	0	2	66	14	78	9.22E-16	75.1

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021916.01.T01	Q1G309	55.385	65	29	0	2	66	8	72	1.17E-12	59.7

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021916.01.T01	A0A1U7ZDW0	59.091	66	27	0	1	66	7	72	2.54e-20	85.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480021916.01	MsG0480021927.01	0.802522	5.391753e-49	2.646646e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021916.01.T01	MTR_2g015540	52.308	65	31	2	66	15	79	4.66e-19	73.6
MsG0480021916.01.T01	MTR_2g090695	70.769	65	19	2	66	13	77	3.21e-18	71.2
MsG0480021916.01.T01	MTR_4g078840	87.692	65	8	2	66	14	78	1.11e-17	70.1
MsG0480021916.01.T01	MTR_4g079680	46.970	66	35	1	66	11	76	5.08e-16	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021916.01.T01	AT2G20562	55.385	65	29	0	2	66	8	72	1.20e-13	59.7
MsG0480021916.01.T01	AT2G31090	55.385	65	29	0	2	66	10	74	4.67e-13	58.2

Find 15 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTTATAGGTGCCATTATT+TGG	0.130092	4:+65958057	MsG0480021916.01.T01:CDS
GTTAACAAAATTCCTGTTAG+AGG	0.310920	4:+65958325	MsG0480021916.01.T01:CDS
AGACCTTTGGGTTTCTTCAT+TGG	0.312975	4:+65958003	MsG0480021916.01.T02:CDS
GCTTGATGTTGCTGTATGTT+TGG	0.367654	4:+65958270	MsG0480021916.01.T01:CDS
AATAGTACTTATCCTCTAAC+AGG	0.383681	4:-65958337	None:intergenic
TTCCAGTTAGAATTCTTAGA+TGG	0.387506	4:+65958299	MsG0480021916.01.T01:CDS
AACCATCTAAGAATTCTAAC+TGG	0.391220	4:-65958301	None:intergenic
GGTGCCATTATTTGGATTCT+CGG	0.429725	4:+65958065	MsG0480021916.01.T01:CDS
GTGCCATTATTTGGATTCTC+GGG	0.441384	4:+65958066	MsG0480021916.01.T01:CDS
ATACCCGAGAATCCAAATAA+TGG	0.476544	4:-65958069	None:intergenic
CCTTGTTGCATATGCTGCAC+AGG	0.484811	4:+65958244	MsG0480021916.01.T01:CDS
GTCTACATTCACAAAAGTCT+TGG	0.513894	4:-65957984	None:intergenic
AATTAATGATAATAGAGCAA+AGG	0.624061	4:-65958030	None:intergenic
CCTGTGCAGCATATGCAACA+AGG	0.629974	4:-65958244	None:intergenic
AATCCAATGAAGAAACCCAA+AGG	0.671262	4:-65958006	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCATTAATTTTATCTCTTAT+AGG	+	Chr4:65958044-65958063	MsG0480021916.01.T01:CDS	15.0%
!!	AATTAATGATAATAGAGCAA+AGG	-	Chr4:65958033-65958052	None:intergenic	20.0%
!!	ACTACAAAAACATAGAAAAT+GGG	-	Chr4:65958120-65958139	None:intergenic	20.0%
!!	TACTACAAAAACATAGAAAA+TGG	-	Chr4:65958121-65958140	None:intergenic	20.0%
!!	TTGTTCATCTTCTTTATTAT+AGG	+	Chr4:65958200-65958219	MsG0480021916.01.T01:intron	20.0%
	AACCATCTAAGAATTCTAAC+TGG	-	Chr4:65958304-65958323	None:intergenic	30.0%
	GTTAACAAAATTCCTGTTAG+AGG	+	Chr4:65958325-65958344	MsG0480021916.01.T01:CDS	30.0%
	TTCCAGTTAGAATTCTTAGA+TGG	+	Chr4:65958299-65958318	MsG0480021916.01.T01:CDS	30.0%
!	TTTTGTGAATGTAGACCTTT+GGG	+	Chr4:65957991-65958010	MsG0480021916.01.T02:CDS	30.0%
	AATCCAATGAAGAAACCCAA+AGG	-	Chr4:65958009-65958028	None:intergenic	35.0%
	ATACCCGAGAATCCAAATAA+TGG	-	Chr4:65958072-65958091	None:intergenic	35.0%
	GTCTACATTCACAAAAGTCT+TGG	-	Chr4:65957987-65958006	None:intergenic	35.0%
!	CTTTTGTGAATGTAGACCTT+TGG	+	Chr4:65957990-65958009	MsG0480021916.01.T02:CDS	35.0%
!!	CTCTTATAGGTGCCATTATT+TGG	+	Chr4:65958057-65958076	MsG0480021916.01.T01:CDS	35.0%
	GCTTGATGTTGCTGTATGTT+TGG	+	Chr4:65958270-65958289	MsG0480021916.01.T01:CDS	40.0%
!	AGACCTTTGGGTTTCTTCAT+TGG	+	Chr4:65958003-65958022	MsG0480021916.01.T02:CDS	40.0%
!	GTGCCATTATTTGGATTCTC+GGG	+	Chr4:65958066-65958085	MsG0480021916.01.T01:CDS	40.0%
!!	GGTGCCATTATTTGGATTCT+CGG	+	Chr4:65958065-65958084	MsG0480021916.01.T01:CDS	40.0%
	CCTGTGCAGCATATGCAACA+AGG	-	Chr4:65958247-65958266	None:intergenic	50.0%
	CCTTGTTGCATATGCTGCAC+AGG	+	Chr4:65958244-65958263	MsG0480021916.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	65957973	65958351	65957973	ID=MsG0480021916.01;Name=MsG0480021916.01
Chr4	mRNA	65958008	65958351	65958008	ID=MsG0480021916.01.T01;Parent=MsG0480021916.01;Name=MsG0480021916.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|0\|0\|1\|0\|0\|2\|0\|69
Chr4	exon	65958008	65958087	65958008	ID=MsG0480021916.01.T01:exon:18222;Parent=MsG0480021916.01.T01
Chr4	exon	65958222	65958351	65958222	ID=MsG0480021916.01.T01:exon:18223;Parent=MsG0480021916.01.T01
Chr4	CDS	65958008	65958087	65958008	ID=MsG0480021916.01.T01:cds;Parent=MsG0480021916.01.T01
Chr4	CDS	65958222	65958351	65958222	ID=MsG0480021916.01.T01:cds;Parent=MsG0480021916.01.T01
Chr4	mRNA	65957973	65958050	65957973	ID=MsG0480021916.01.T02;Parent=MsG0480021916.01;Name=MsG0480021916.01.T02;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|25
Chr4	exon	65957973	65958050	65957973	ID=MsG0480021916.01.T02:exon:18224;Parent=MsG0480021916.01.T02
Chr4	CDS	65957973	65958050	65957973	ID=MsG0480021916.01.T02:cds;Parent=MsG0480021916.01.T02

Gene Sequence	>MsG0480021916.01.T02 ATGGCAAGTGACCAAGACTTTTGTGAATGTAGACCTTTGGGTTTCTTCATTGGATTACCTTTGCTCTATTATCATTAA >MsG0480021916.01.T01 TTTGGGTTTCTTCATTGGATTACCTTTGCTCTATTATCATTAATTTTATCTCTTATAGGTGCCATTATTTGGATTCTCGGGTCAATATTGAGTTGCTGCTGTCCTTGTTGCATATGCTGCACAGGATTGCTTGATGTTGCTGTATGTTTGGTAAAGCTTCCAGTTAGAATTCTTAGATGGTTTGTTAACAAAATTCCTGTTAGAGGATAA
Protein sequence	>MsG0480021916.01.T02 MASDQDFCECRPLGFFIGLPLLYYH* >MsG0480021916.01.T01 FGFLHWITFALLSLILSLIGAIIWILGSILSCCCPCCICCTGLLDVAVCLVKLPVRILRWFVNKIPVRG*