AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021935.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021935.01.T01 MTR_4g079190 81.250 288 20 4 1 277 19 283 5.65e-145 408
MsG0480021935.01.T01 MTR_2g103560 64.968 314 72 2 1 276 19 332 3.85e-127 365
MsG0480021935.01.T01 MTR_1g048860 40.816 98 28 1 109 176 2 99 2.58e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021935.01.T01 AT1G23280 72.571 175 46 1 1 175 19 191 4.57e-90 270

Find 49 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAGATGATATAGAAATTT+TGG 0.202339 4:-66161635 MsG0480021935.01.T01:CDS
GACTTTAGCTTCAAAGAAAC+TGG 0.316024 4:-66160424 MsG0480021935.01.T01:CDS
TATCCGCATCTGAGTCATTT+TGG 0.319490 4:+66162357 None:intergenic
CAAATTCCGGTGACATTGTA+AGG 0.322485 4:+66163597 None:intergenic
TGAACTTCGGTTACAAATTC+CGG 0.346804 4:+66163584 None:intergenic
GTCTATTGAGAAAGAGCTAT+TGG 0.348403 4:-66161945 MsG0480021935.01.T01:CDS
TTAATCAAAGGAAAGCAAAA+AGG 0.387263 4:-66160452 MsG0480021935.01.T01:CDS
ATCTTGTGTATAAGAAGCTT+GGG 0.406860 4:+66162394 None:intergenic
TACTGCACTGACAGATGTAT+TGG 0.411812 4:-66162417 MsG0480021935.01.T01:intron
ACTCTTTCCCACAAATCCTT+TGG 0.416287 4:+66162582 None:intergenic
TATCTTGTGTATAAGAAGCT+TGG 0.432339 4:+66162393 None:intergenic
TGCATATATCACCTCCTGTC+TGG 0.447878 4:+66161900 None:intergenic
TGTTGAAGAAGAGGATGAAA+TGG 0.458882 4:-66161822 MsG0480021935.01.T01:intron
CTCATACGCCAAAGGATTTG+TGG 0.458884 4:-66162590 MsG0480021935.01.T01:CDS
ATTGAAGAGGAAACAGAAAC+AGG 0.469123 4:-66161682 MsG0480021935.01.T01:CDS
AGCTTCAAAGAAACTGGAAA+AGG 0.487427 4:-66160418 MsG0480021935.01.T01:CDS
ATCTCAAGCTGTTGAAGAAG+AGG 0.492311 4:-66161831 MsG0480021935.01.T01:CDS
AACGACACTGCCAAGAAAAG+AGG 0.508652 4:-66162107 MsG0480021935.01.T01:CDS
TGAAGTCATTGACAAACATC+TGG 0.510638 4:-66162529 MsG0480021935.01.T01:intron
CATAGCGACTATTAGCTAAA+GGG 0.510834 4:+66163559 None:intergenic
AAAAGCAGCTGTGCTAGAAA+AGG 0.513340 4:-66162050 MsG0480021935.01.T01:intron
GCATAGCGACTATTAGCTAA+AGG 0.516741 4:+66163558 None:intergenic
CTCAGATGCGGATACGTATG+AGG 0.518871 4:-66162348 MsG0480021935.01.T01:CDS
AGAATATGTTGAATTTGAAG+AGG 0.522267 4:-66161656 MsG0480021935.01.T01:CDS
CAATGACTTCAAGTGCCTGC+TGG 0.525481 4:+66162541 None:intergenic
TCATACGCCAAAGGATTTGT+GGG 0.528939 4:-66162589 MsG0480021935.01.T01:CDS
AGTGCCTGCTGGTAATTCCT+AGG 0.529178 4:+66162552 None:intergenic
TTTCTTTGATTTAGAATCAG+AGG 0.543640 4:+66160392 None:intergenic
GGGAAAGAGTTAAGCTGCCT+AGG 0.548712 4:-66162569 MsG0480021935.01.T01:CDS
GGAAACTTTCTTTGAAAACA+AGG 0.556860 4:-66162327 MsG0480021935.01.T01:intron
TAGCTAAAGGGCATGAACTT+CGG 0.561734 4:+66163571 None:intergenic
ACCATTGTCTTCACGAATGG+TGG 0.572329 4:+66163536 None:intergenic
CAAAACGGACTTTACCAGAC+AGG 0.572560 4:-66161914 MsG0480021935.01.T01:CDS
AAACTGAAGCACTTAATCAA+AGG 0.580098 4:-66160464 MsG0480021935.01.T01:CDS
AGTACCATTGTCTTCACGAA+TGG 0.580983 4:+66163533 None:intergenic
AAATTCCGGTGACATTGTAA+GGG 0.585183 4:+66163598 None:intergenic
CTATTGGTACGCCTTCAAAA+CGG 0.597593 4:-66161929 MsG0480021935.01.T01:CDS
AGAAACCCTTACAATGTCAC+CGG 0.609253 4:-66163603 MsG0480021935.01.T01:CDS
GAAAGCGAAGGTCCTCATTG+AGG 0.612298 4:-66160373 MsG0480021935.01.T01:intron
TTCTAAATCAAAGAAAGCGA+AGG 0.616150 4:-66160385 MsG0480021935.01.T01:CDS
AACACGACGATGCTGTCGAA+AGG 0.621867 4:-66159167 MsG0480021935.01.T01:CDS
GCTGCCTAGGAATTACCAGC+AGG 0.624895 4:-66162556 MsG0480021935.01.T01:CDS
TGAAAGAGCTCATACGCCAA+AGG 0.633610 4:-66162598 MsG0480021935.01.T01:CDS
AGTATAAAATTACCTCAATG+AGG 0.639733 4:+66160361 None:intergenic
TACATGGCCAAAATTACCAC+CGG 0.640975 4:-66163636 MsG0480021935.01.T01:CDS
GCCACCATTCGTGAAGACAA+TGG 0.656349 4:-66163537 MsG0480021935.01.T01:intron
TGCTGTCGAAAGGCAAAGAG+CGG 0.666319 4:-66159157 MsG0480021935.01.T01:CDS
AACGGACTTTACCAGACAGG+AGG 0.681084 4:-66161911 MsG0480021935.01.T01:CDS
TGACCAAAATGACTCAGATG+CGG 0.682645 4:-66162360 MsG0480021935.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAATTATAAATTTTTTT+TGG - Chr4:66163332-66163351 MsG0480021935.01.T01:intron 0.0%
!!! AAAAAATTCTAATTTTTTTC+AGG + Chr4:66163239-66163258 None:intergenic 10.0%
!!! ACATAAATTAATTTGAAAAT+AGG + Chr4:66163166-66163185 None:intergenic 10.0%
!!! CATAAATTAATTTGAAAATA+GGG + Chr4:66163165-66163184 None:intergenic 10.0%
!!! TATAATATTTTGAATTTTTC+TGG - Chr4:66163364-66163383 MsG0480021935.01.T01:intron 10.0%
!! AAAAAGTTGACATATTTATA+GGG - Chr4:66163508-66163527 MsG0480021935.01.T01:intron 15.0%
!! AAAAAGTTGACATATTTATA+GGG + Chr4:66163527-66163508 None:intergenic 15.0%
!! ATACAAAAAAGTTATTGATA+TGG - Chr4:66160921-66160940 MsG0480021935.01.T01:intron 15.0%
!! ATTTGATTAAAATGACATTT+AGG - Chr4:66159408-66159427 MsG0480021935.01.T01:intron 15.0%
!!! AAATGTCATTTTAATCAAAT+GGG + Chr4:66159408-66159427 None:intergenic 15.0%
!!! AATAATAAGTTGATAGTTTA+TGG - Chr4:66160091-66160110 MsG0480021935.01.T01:intron 15.0%
!!! AATATATTTGAAATAGGTTA+TGG - Chr4:66161285-66161304 MsG0480021935.01.T01:intron 15.0%
!!! ATAATGGAAGATAATAAAAT+GGG + Chr4:66162137-66162156 None:intergenic 15.0%
!!! ATATGAGTTTACTTTAAAAA+AGG - Chr4:66163416-66163435 MsG0480021935.01.T01:intron 15.0%
!!! TAAATGTCATTTTAATCAAA+TGG + Chr4:66159409-66159428 None:intergenic 15.0%
!!! TATGAGTTTACTTTAAAAAA+GGG - Chr4:66163417-66163436 MsG0480021935.01.T01:intron 15.0%
!!! TGAAGATTGAAAATTTTATA+GGG - Chr4:66163448-66163467 MsG0480021935.01.T01:intron 15.0%
!!! TGCTAAAATATATTTGAAAT+AGG - Chr4:66161279-66161298 MsG0480021935.01.T01:intron 15.0%
!! AAAGATAAAAGAAATATACC+AGG + Chr4:66161076-66161095 None:intergenic 20.0%
!! AAATTCTTAGTAACTTAACT+TGG - Chr4:66161038-66161057 MsG0480021935.01.T01:intron 20.0%
!! AACATTCTGTTATAATTTGA+AGG - Chr4:66162589-66162608 MsG0480021935.01.T01:CDS 20.0%
!! AACTAAAATACAAAAGATTC+TGG - Chr4:66161364-66161383 MsG0480021935.01.T01:intron 20.0%
!! ATATAGTTATGAAATTCTTG+TGG - Chr4:66159323-66159342 MsG0480021935.01.T01:intron 20.0%
!! ATTAAAAAGCAATAACATGA+TGG - Chr4:66162259-66162278 MsG0480021935.01.T01:intron 20.0%
!! CAAAAAGTTGACATATTTAT+AGG - Chr4:66163507-66163526 MsG0480021935.01.T01:intron 20.0%
!! CAAAAAGTTGACATATTTAT+AGG + Chr4:66163526-66163507 None:intergenic 20.0%
!! CTTGATAAATAAGAATCAAA+AGG + Chr4:66161948-66161967 None:intergenic 20.0%
!! GTAAATAGATGAGTAAAAAA+TGG + Chr4:66161904-66161923 None:intergenic 20.0%
!! TAAAATAATCATCCATAGTA+TGG + Chr4:66161683-66161702 None:intergenic 20.0%
!! TATAGTTATGAAATTCTTGT+GGG - Chr4:66159324-66159343 MsG0480021935.01.T01:intron 20.0%
!! TTATTGTTAAAAAGAAGCAA+AGG + Chr4:66160497-66160516 None:intergenic 20.0%
!!! AAAACATATTTAACCCTTTT+AGG + Chr4:66163047-66163066 None:intergenic 20.0%
!!! AAAAGGGTTAAATATGTTTT+TGG - Chr4:66163047-66163066 MsG0480021935.01.T01:intron 20.0%
!!! AAATTTTCAATCTTCACTTT+TGG + Chr4:66163444-66163463 None:intergenic 20.0%
!!! AGTGAATTTTGTCTATTTTT+TGG + Chr4:66162074-66162093 None:intergenic 20.0%
!!! CTTTTGATTCTTATTTATCA+AGG - Chr4:66161946-66161965 MsG0480021935.01.T01:CDS 20.0%
!!! GATAATGGAAGATAATAAAA+TGG + Chr4:66162138-66162157 None:intergenic 20.0%
!!! GTGAAGATTGAAAATTTTAT+AGG - Chr4:66163447-66163466 MsG0480021935.01.T01:intron 20.0%
!!! GTGAATTTTGTCTATTTTTT+GGG + Chr4:66162073-66162092 None:intergenic 20.0%
!!! TAAAATTTTCCTTCAACTTT+TGG - Chr4:66163107-66163126 MsG0480021935.01.T01:intron 20.0%
!!! TATTACATTTTCCATTCAAA+AGG + Chr4:66160075-66160094 None:intergenic 20.0%
!!! TATTTTATCTTTTCCTTGTT+TGG - Chr4:66161696-66161715 MsG0480021935.01.T01:intron 20.0%
!!! TCCTTTATCTACTATATTTT+AGG - Chr4:66160562-66160581 MsG0480021935.01.T01:intron 20.0%
!!! TTTAGAACTTACGAAATTTT+AGG + Chr4:66163556-66163575 None:intergenic 20.0%
!!! TTTTGATTTTCATTGTTTTC+AGG - Chr4:66163589-66163608 MsG0480021935.01.T01:CDS 20.0%
! AAAATCAAAATTGTGATGTC+AGG + Chr4:66163579-66163598 None:intergenic 25.0%
! AAATATTTCTTCACTCATCA+AGG - Chr4:66161320-66161339 MsG0480021935.01.T01:intron 25.0%
! AATATTCAAAAGAAACAGTG+AGG + Chr4:66160812-66160831 None:intergenic 25.0%
! ACAATGCAAATGATAGAAAA+TGG - Chr4:66161642-66161661 MsG0480021935.01.T01:CDS 25.0%
! ACTTTGATATCTTAGACATT+TGG - Chr4:66159446-66159465 MsG0480021935.01.T01:intron 25.0%
! AGAATATGTTGAATTTGAAG+AGG - Chr4:66161126-66161145 MsG0480021935.01.T01:intron 25.0%
! AGTATAAAATTACCTCAATG+AGG + Chr4:66162424-66162443 None:intergenic 25.0%
! ATAACATAATTTAGTACGCA+CGG + Chr4:66160780-66160799 None:intergenic 25.0%
! CAAAAAAGTTATTGATATGG+TGG - Chr4:66160924-66160943 MsG0480021935.01.T01:intron 25.0%
! CAATGCAAATGATAGAAAAT+GGG - Chr4:66161643-66161662 MsG0480021935.01.T01:CDS 25.0%
! CACAATAGCATTTAAAAGAA+CGG + Chr4:66162467-66162486 None:intergenic 25.0%
! CATGTCAATGATTATGATTA+AGG - Chr4:66159512-66159531 MsG0480021935.01.T01:intron 25.0%
! GAAAATAGTTATCCATACTA+TGG - Chr4:66161668-66161687 MsG0480021935.01.T01:CDS 25.0%
! GCCTAAAATATAGTAGATAA+AGG + Chr4:66160566-66160585 None:intergenic 25.0%
! GTGTGTTTGACATAATTATT+TGG + Chr4:66162818-66162837 None:intergenic 25.0%
! TTAATCAAAGGAAAGCAAAA+AGG - Chr4:66162330-66162349 MsG0480021935.01.T01:CDS 25.0%
! TTGGGAGAACTTATAAAAAA+GGG + Chr4:66159974-66159993 None:intergenic 25.0%
!! AAGTTGAAGGAAAATTTTAG+AGG - Chr4:66163476-66163495 MsG0480021935.01.T01:intron 25.0%
!! AAGTTGAAGGAAAATTTTAG+AGG + Chr4:66163495-66163476 None:intergenic 25.0%
!! AGTTGAAGGAAAATTTTAGA+GGG - Chr4:66163477-66163496 MsG0480021935.01.T01:intron 25.0%
!! AGTTGAAGGAAAATTTTAGA+GGG + Chr4:66163496-66163477 None:intergenic 25.0%
!! ATTTTCCAAAATCACTAACA+AGG + Chr4:66162222-66162241 None:intergenic 25.0%
!! GATGTTTTCATAAGTTATCT+TGG - Chr4:66159721-66159740 MsG0480021935.01.T01:intron 25.0%
!! GGAAGATGATATAGAAATTT+TGG - Chr4:66161147-66161166 MsG0480021935.01.T01:intron 25.0%
!! TTTATCTTTTCAGATTGAAG+AGG - Chr4:66161087-66161106 MsG0480021935.01.T01:intron 25.0%
!! TTTCAATAGTTTTCATGTAG+AGG + Chr4:66160169-66160188 None:intergenic 25.0%
!! TTTCTTTGATTTAGAATCAG+AGG + Chr4:66162393-66162412 None:intergenic 25.0%
!! TTTTCATAAGTTATCTTGGA+GGG - Chr4:66159725-66159744 MsG0480021935.01.T01:intron 25.0%
!!! AACTTACGAAATTTTAGGTA+GGG + Chr4:66163551-66163570 None:intergenic 25.0%
!!! AATCTTCTTTTTTGCTTGTT+CGG + Chr4:66162030-66162049 None:intergenic 25.0%
!!! GCTGTTTTAATAAGTTATCT+TGG - Chr4:66159680-66159699 MsG0480021935.01.T01:intron 25.0%
AAGTTATCTTGGAGAGTTTA+TGG - Chr4:66159691-66159710 MsG0480021935.01.T01:intron 30.0%
AGATGTATGAGATACCTAAA+AGG - Chr4:66163030-66163049 MsG0480021935.01.T01:intron 30.0%
AGGGTTTCTACAAAAAATAC+CGG + Chr4:66159168-66159187 None:intergenic 30.0%
AGTGTTCTTCTCAAATGATA+AGG + Chr4:66162197-66162216 None:intergenic 30.0%
ATAAAATGGGCAGAAAGTAA+AGG + Chr4:66162124-66162143 None:intergenic 30.0%
ATCTTGTGTATAAGAAGCTT+GGG + Chr4:66160391-66160410 None:intergenic 30.0%
ATTATGATTAAGGCTCTGTT+TGG - Chr4:66159522-66159541 MsG0480021935.01.T01:intron 30.0%
ATTGCAGCTTATATATCATG+AGG - Chr4:66159579-66159598 MsG0480021935.01.T01:intron 30.0%
CATTTGGATGCAAAAGATAA+TGG + Chr4:66162153-66162172 None:intergenic 30.0%
CTTTGAGCTGTAGTAAATAT+TGG - Chr4:66161465-66161484 MsG0480021935.01.T01:intron 30.0%
GATGTATGAGATACCTAAAA+GGG - Chr4:66163031-66163050 MsG0480021935.01.T01:intron 30.0%
GTTGGGAGAACTTATAAAAA+AGG + Chr4:66159975-66159994 None:intergenic 30.0%
TAACATAATTTAGTACGCAC+GGG + Chr4:66160779-66160798 None:intergenic 30.0%
TAACCGCAATATTACAACAA+AGG + Chr4:66162840-66162859 None:intergenic 30.0%
TATCTTGTGTATAAGAAGCT+TGG + Chr4:66160392-66160411 None:intergenic 30.0%
TGGGAGAACTTATAAAAAAG+GGG + Chr4:66159973-66159992 None:intergenic 30.0%
TTATAGGGACTAAAAGTTGA+AGG - Chr4:66163463-66163482 MsG0480021935.01.T01:intron 30.0%
TTCTAAATCAAAGAAAGCGA+AGG - Chr4:66162397-66162416 MsG0480021935.01.T01:CDS 30.0%
! AAAAATACCGGTGGTAATTT+TGG + Chr4:66159156-66159175 None:intergenic 30.0%
! AAACTGAAGCACTTAATCAA+AGG - Chr4:66162318-66162337 MsG0480021935.01.T01:intron 30.0%
! ACACCTTTGTTGTAATATTG+CGG - Chr4:66162834-66162853 MsG0480021935.01.T01:intron 30.0%
! GGAAACTTTCTTTGAAAACA+AGG - Chr4:66160455-66160474 MsG0480021935.01.T01:CDS 30.0%
! GGTAGGGTTAAATATGTTTT+TGG + Chr4:66163535-66163554 None:intergenic 30.0%
! GTTTTCATAAGTTATCTTGG+AGG - Chr4:66159724-66159743 MsG0480021935.01.T01:intron 30.0%
! TTGTATCGTATCCTTTTGAA+TGG - Chr4:66160061-66160080 MsG0480021935.01.T01:intron 30.0%
!! GAACTTACGAAATTTTAGGT+AGG + Chr4:66163552-66163571 None:intergenic 30.0%
!! TGATTTTCATTGTTTTCAGG+TGG - Chr4:66163592-66163611 MsG0480021935.01.T01:CDS 30.0%
!! TTATCTTTTCCTTGTTTGGT+TGG - Chr4:66161700-66161719 MsG0480021935.01.T01:intron 30.0%
!!! TTGGTTGTTGTTGTTTTACA+GGG - Chr4:66160645-66160664 MsG0480021935.01.T01:intron 30.0%
AAATTCCGGTGACATTGTAA+GGG + Chr4:66159187-66159206 None:intergenic 35.0%
AACAACCAACATGATCAATC+AGG + Chr4:66160634-66160653 None:intergenic 35.0%
ACAATTGCATTGTGTGATGT+AGG - Chr4:66160134-66160153 MsG0480021935.01.T01:intron 35.0%
ACCAAAATTCCCAAAAGAAG+TGG + Chr4:66162540-66162559 None:intergenic 35.0%
AGCTTCAAAGAAACTGGAAA+AGG - Chr4:66162364-66162383 MsG0480021935.01.T01:CDS 35.0%
AGGATACGATACAACCATTT+AGG + Chr4:66160055-66160074 None:intergenic 35.0%
ATAAGAATCAAAAGGATCCC+AGG + Chr4:66161940-66161959 None:intergenic 35.0%
ATGGCATTTACTAATGCAGA+TGG - Chr4:66162278-66162297 MsG0480021935.01.T01:intron 35.0%
ATGTGGGGTAGGTAATAATT+TGG - Chr4:66159475-66159494 MsG0480021935.01.T01:intron 35.0%
ATTGAAGAGGAAACAGAAAC+AGG - Chr4:66161100-66161119 MsG0480021935.01.T01:intron 35.0%
CATAGCGACTATTAGCTAAA+GGG + Chr4:66159226-66159245 None:intergenic 35.0%
CTATCTAAACAGACCCTAAA+TGG - Chr4:66160038-66160057 MsG0480021935.01.T01:intron 35.0%
CTCGTGTTCGAAACAATTAA+GGG - Chr4:66162865-66162884 MsG0480021935.01.T01:intron 35.0%
GGATACGATACAACCATTTA+GGG + Chr4:66160054-66160073 None:intergenic 35.0%
GTCTATTGAGAAAGAGCTAT+TGG - Chr4:66160837-66160856 MsG0480021935.01.T01:intron 35.0%
GTGGAGAAAACTGCATTATT+TGG - Chr4:66159348-66159367 MsG0480021935.01.T01:intron 35.0%
GTTTCTACAAAAAATACCGG+TGG + Chr4:66159165-66159184 None:intergenic 35.0%
TAAATGATGCTGCTACAAAC+TGG - Chr4:66162632-66162651 MsG0480021935.01.T01:intron 35.0%
TACAATACACCCAAAATGGT+GGG - Chr4:66159884-66159903 MsG0480021935.01.T01:intron 35.0%
TAGAAGCTTTGCAATATGCA+TGG - Chr4:66161233-66161252 MsG0480021935.01.T01:intron 35.0%
TAGCAGTTTCTCCATAAAAG+TGG - Chr4:66160608-66160627 MsG0480021935.01.T01:intron 35.0%
TCTCGTGTTCGAAACAATTA+AGG - Chr4:66162864-66162883 MsG0480021935.01.T01:intron 35.0%
TGAACTTCGGTTACAAATTC+CGG + Chr4:66159201-66159220 None:intergenic 35.0%
TGAAGTCATTGACAAACATC+TGG - Chr4:66160253-66160272 MsG0480021935.01.T01:intron 35.0%
TGTTGAAGAAGAGGATGAAA+TGG - Chr4:66160960-66160979 MsG0480021935.01.T01:intron 35.0%
TTATAGGGACCAAAAGTTGA+AGG + Chr4:66163119-66163138 None:intergenic 35.0%
TTATGAAATTCTTGTGGGTG+TGG - Chr4:66159329-66159348 MsG0480021935.01.T01:intron 35.0%
! AACAACTCAACCACTTCTTT+TGG - Chr4:66162527-66162546 MsG0480021935.01.T01:intron 35.0%
! ACAACTCAACCACTTCTTTT+GGG - Chr4:66162528-66162547 MsG0480021935.01.T01:intron 35.0%
! ACTCTCCTTGTTAGTGATTT+TGG - Chr4:66162214-66162233 MsG0480021935.01.T01:intron 35.0%
! AGGGAAATTTTGCTAATCAG+AGG - Chr4:66161742-66161761 MsG0480021935.01.T01:intron 35.0%
! CATTTAGGGTCTGTTTAGAT+AGG + Chr4:66160040-66160059 None:intergenic 35.0%
! CTTTTATGGAGAAACTGCTA+TGG + Chr4:66160608-66160627 None:intergenic 35.0%
! GACTTTAGCTTCAAAGAAAC+TGG - Chr4:66162358-66162377 MsG0480021935.01.T01:CDS 35.0%
! GATTATTTGAGTTTGTCTGC+TGG + Chr4:66160018-66160037 None:intergenic 35.0%
! GATTCTGGATTAGATATGCA+TGG - Chr4:66161379-66161398 MsG0480021935.01.T01:intron 35.0%
! TCTCTCTTAACCTCTTTTCT+TGG + Chr4:66160688-66160707 None:intergenic 35.0%
! TTTTTCTTCTCATGTCGTGA+TGG - Chr4:66161774-66161793 MsG0480021935.01.T01:intron 35.0%
!! ACCACTTCTTTTGGGAATTT+TGG - Chr4:66162536-66162555 MsG0480021935.01.T01:CDS 35.0%
!! AGACATTTGGTTCTTCATGT+GGG - Chr4:66159459-66159478 MsG0480021935.01.T01:intron 35.0%
!! TAGACATTTGGTTCTTCATG+TGG - Chr4:66159458-66159477 MsG0480021935.01.T01:intron 35.0%
!! TTTTCCTTGTTTGGTTGGTT+GGG - Chr4:66161705-66161724 MsG0480021935.01.T01:intron 35.0%
!!! CTTACCCTGTTTTTTTACAC+AGG + Chr4:66159858-66159877 None:intergenic 35.0%
!!! GTTGGTTGTTGTTGTTTTAC+AGG - Chr4:66160644-66160663 MsG0480021935.01.T01:intron 35.0%
!!! TTCTTTTTTGCTTGTTCGGT+TGG + Chr4:66162026-66162045 None:intergenic 35.0%
AAAAGCAGCTGTGCTAGAAA+AGG - Chr4:66160732-66160751 MsG0480021935.01.T01:intron 40.0%
AAGTTATCTTGGAGGGCTTA+TGG - Chr4:66159732-66159751 MsG0480021935.01.T01:intron 40.0%
AATGGCAGCGATGATGAAAA+TGG - Chr4:66161198-66161217 MsG0480021935.01.T01:intron 40.0%
ACTCTTTCCCACAAATCCTT+TGG + Chr4:66160203-66160222 None:intergenic 40.0%
AGAAACCCTTACAATGTCAC+CGG - Chr4:66159179-66159198 MsG0480021935.01.T01:intron 40.0%
AGCCTCCTGTGTAAAAAAAC+AGG - Chr4:66159850-66159869 MsG0480021935.01.T01:intron 40.0%
AGTACCATTGTCTTCACGAA+TGG + Chr4:66159252-66159271 None:intergenic 40.0%
AGTTGTCTCTTCACTTGAGA+GGG + Chr4:66161852-66161871 None:intergenic 40.0%
ATCTCAAGCTGTTGAAGAAG+AGG - Chr4:66160951-66160970 MsG0480021935.01.T01:intron 40.0%
ATGGAGGGACGAAATATCAA+GGG - Chr4:66161793-66161812 MsG0480021935.01.T01:intron 40.0%
CAAATTCCGGTGACATTGTA+AGG + Chr4:66159188-66159207 None:intergenic 40.0%
CATCCAAATGAGACATATGC+TGG - Chr4:66162163-66162182 MsG0480021935.01.T01:intron 40.0%
CTATTGGTACGCCTTCAAAA+CGG - Chr4:66160853-66160872 MsG0480021935.01.T01:intron 40.0%
GATATGACTGCAGTATCTAG+AGG + Chr4:66161549-66161568 None:intergenic 40.0%
GCATAGCGACTATTAGCTAA+AGG + Chr4:66159227-66159246 None:intergenic 40.0%
GCCTCCTGTGTAAAAAAACA+GGG - Chr4:66159851-66159870 MsG0480021935.01.T01:intron 40.0%
GTACAATACACCCAAAATGG+TGG - Chr4:66159883-66159902 MsG0480021935.01.T01:intron 40.0%
GTGACTGCTTGAGTTCTATA+TGG - Chr4:66162942-66162961 MsG0480021935.01.T01:intron 40.0%
GTTGTTGTCACATCACTGAA+AGG - Chr4:66159804-66159823 MsG0480021935.01.T01:intron 40.0%
TACATCTGTCAGTGCAGTAA+AGG + Chr4:66160364-66160383 None:intergenic 40.0%
TAGTTGTCTCTTCACTTGAG+AGG + Chr4:66161853-66161872 None:intergenic 40.0%
TCATACGCCAAAGGATTTGT+GGG - Chr4:66160193-66160212 MsG0480021935.01.T01:intron 40.0%
TGACCAAAATGACTCAGATG+CGG - Chr4:66160422-66160441 MsG0480021935.01.T01:CDS 40.0%
TGCGTACAATACACCCAAAA+TGG - Chr4:66159880-66159899 MsG0480021935.01.T01:intron 40.0%
TTTGCAATTCACGAGTCACA+GGG - Chr4:66161174-66161193 MsG0480021935.01.T01:intron 40.0%
! ACTTGGTAATATGTTGAGCC+TGG - Chr4:66161055-66161074 MsG0480021935.01.T01:intron 40.0%
! TACTGCACTGACAGATGTAT+TGG - Chr4:66160365-66160384 MsG0480021935.01.T01:intron 40.0%
! TAGCTAAAGGGCATGAACTT+CGG + Chr4:66159214-66159233 None:intergenic 40.0%
! TATCCGCATCTGAGTCATTT+TGG + Chr4:66160428-66160447 None:intergenic 40.0%
! TCTGGTAAAGTCCGTTTTGA+AGG + Chr4:66160867-66160886 None:intergenic 40.0%
! TTGGTTGGTTGGGTAGAAAA+CGG - Chr4:66161715-66161734 MsG0480021935.01.T01:intron 40.0%
! TTTTGCAATTCACGAGTCAC+AGG - Chr4:66161173-66161192 MsG0480021935.01.T01:intron 40.0%
!! CTTTTCCTTGTTTGGTTGGT+TGG - Chr4:66161704-66161723 MsG0480021935.01.T01:intron 40.0%
!! GACATTTGGTTCTTCATGTG+GGG - Chr4:66159460-66159479 MsG0480021935.01.T01:intron 40.0%
!!! ACCCTGTTTTTTTACACAGG+AGG + Chr4:66159855-66159874 None:intergenic 40.0%
!!! GATCAATCAGGCCACTTTTA+TGG + Chr4:66160622-66160641 None:intergenic 40.0%
AACGACACTGCCAAGAAAAG+AGG - Chr4:66160675-66160694 MsG0480021935.01.T01:intron 45.0%
AAGGTCACACGTTCAAGTCT+TGG - Chr4:66159823-66159842 MsG0480021935.01.T01:intron 45.0%
AATGAGACATATGCTGGAGG+CGG - Chr4:66162169-66162188 MsG0480021935.01.T01:intron 45.0%
ACCATTGTCTTCACGAATGG+TGG + Chr4:66159249-66159268 None:intergenic 45.0%
AGAACGGATCGCTTTATACG+AGG + Chr4:66162451-66162470 None:intergenic 45.0%
AGGTCAGCATGCAAAAATGG+AGG + Chr4:66162744-66162763 None:intergenic 45.0%
AGTGGCCTGATTGATCATGT+TGG - Chr4:66160626-66160645 MsG0480021935.01.T01:intron 45.0%
ATTCACGAGTCACAGGGAAA+TGG - Chr4:66161180-66161199 MsG0480021935.01.T01:intron 45.0%
CAAGGGGGAGAAATAATGTG+AGG - Chr4:66161810-66161829 MsG0480021935.01.T01:intron 45.0%
CCAAATGAGACATATGCTGG+AGG - Chr4:66162166-66162185 MsG0480021935.01.T01:intron 45.0%
CCTCCAGCATATGTCTCATT+TGG + Chr4:66162169-66162188 None:intergenic 45.0%
CGACTTCTCTTTGTCTTGCT+TGG - Chr4:66162778-66162797 MsG0480021935.01.T01:intron 45.0%
CTCATACGCCAAAGGATTTG+TGG - Chr4:66160192-66160211 MsG0480021935.01.T01:intron 45.0%
CTTATAAAAAAGGGGCAGCC+CGG + Chr4:66159965-66159984 None:intergenic 45.0%
GATGGAGGGACGAAATATCA+AGG - Chr4:66161792-66161811 MsG0480021935.01.T01:intron 45.0%
GCAAGACAAAGAGAAGTCGT+AGG + Chr4:66162777-66162796 None:intergenic 45.0%
GTGAGGTCAGCATGCAAAAA+TGG + Chr4:66162747-66162766 None:intergenic 45.0%
TAGTACGCACGGGTAACATT+CGG + Chr4:66160769-66160788 None:intergenic 45.0%
TCTACCCAACCAACCAAACA+AGG + Chr4:66161712-66161731 None:intergenic 45.0%
TGAAAGAGCTCATACGCCAA+AGG - Chr4:66160184-66160203 MsG0480021935.01.T01:intron 45.0%
TGCATATATCACCTCCTGTC+TGG + Chr4:66160885-66160904 None:intergenic 45.0%
TGGAGGGACGAAATATCAAG+GGG - Chr4:66161794-66161813 MsG0480021935.01.T01:intron 45.0%
TGTAAATTCTCCCAACGCCT+TGG - Chr4:66159769-66159788 MsG0480021935.01.T01:intron 45.0%
TTCTTCTCATGTCGTGATGG+AGG - Chr4:66161777-66161796 MsG0480021935.01.T01:intron 45.0%
TTGGGTAGAAAACGGGAGAA+GGG - Chr4:66161723-66161742 MsG0480021935.01.T01:intron 45.0%
! CAAAACGGACTTTACCAGAC+AGG - Chr4:66160868-66160887 MsG0480021935.01.T01:intron 45.0%
! TCTTCTCATGTCGTGATGGA+GGG - Chr4:66161778-66161797 MsG0480021935.01.T01:intron 45.0%
! TGGCATAAGCACTTCTGAGA+CGG + Chr4:66159998-66160017 None:intergenic 45.0%
! TGGTTGGTTGGGTAGAAAAC+GGG - Chr4:66161716-66161735 MsG0480021935.01.T01:intron 45.0%
!! AAGTCGTAGGTAGCTTTGTG+AGG + Chr4:66162764-66162783 None:intergenic 45.0%
!! TTTGGTTCTTCATGTGGGGT+AGG - Chr4:66159464-66159483 MsG0480021935.01.T01:intron 45.0%
!!! ATGAATTTTTATATTTTTTA+CGG + Chr4:66163293-66163312 None:intergenic 5.0%
AACACGACGATGCTGTCGAA+AGG - Chr4:66163615-66163634 MsG0480021935.01.T01:CDS 50.0%
ACAACTTTCCCGTTACGCCA+AGG + Chr4:66159789-66159808 None:intergenic 50.0%
AGTGCCTGCTGGTAATTCCT+AGG + Chr4:66160233-66160252 None:intergenic 50.0%
ATGAGACATATGCTGGAGGC+GGG - Chr4:66162170-66162189 MsG0480021935.01.T01:intron 50.0%
CAATGACTTCAAGTGCCTGC+TGG + Chr4:66160244-66160263 None:intergenic 50.0%
CTCAGATGCGGATACGTATG+AGG - Chr4:66160434-66160453 MsG0480021935.01.T01:CDS 50.0%
CTGTCAGTGCAGTAAAGGTC+AGG + Chr4:66160359-66160378 None:intergenic 50.0%
GAAAGCGAAGGTCCTCATTG+AGG - Chr4:66162409-66162428 MsG0480021935.01.T01:intron 50.0%
GCCACCATTCGTGAAGACAA+TGG - Chr4:66159245-66159264 MsG0480021935.01.T01:intron 50.0%
GGAGGGACGAAATATCAAGG+GGG - Chr4:66161795-66161814 MsG0480021935.01.T01:intron 50.0%
GGGAAAGAGTTAAGCTGCCT+AGG - Chr4:66160213-66160232 MsG0480021935.01.T01:intron 50.0%
GTTGGGTAGAAAACGGGAGA+AGG - Chr4:66161722-66161741 MsG0480021935.01.T01:intron 50.0%
TGCTGTCGAAAGGCAAAGAG+CGG - Chr4:66163625-66163644 MsG0480021935.01.T01:CDS 50.0%
TTACCTCGCTCATCTAGCCT+GGG - Chr4:66161920-66161939 MsG0480021935.01.T01:CDS 50.0%
TTTACCTCGCTCATCTAGCC+TGG - Chr4:66161919-66161938 MsG0480021935.01.T01:CDS 50.0%
! AAAATGGTGGGACCCCTTCT+CGG - Chr4:66159896-66159915 MsG0480021935.01.T01:intron 50.0%
! AACGGACTTTACCAGACAGG+AGG - Chr4:66160871-66160890 MsG0480021935.01.T01:intron 50.0%
! AGAAGGGGTCCCACCATTTT+GGG + Chr4:66159896-66159915 None:intergenic 50.0%
!! AAGCACTTCTGAGACGGGTT+GGG + Chr4:66159992-66160011 None:intergenic 50.0%
!! GGCATAAGCACTTCTGAGAC+GGG + Chr4:66159997-66160016 None:intergenic 50.0%
!! TAAGCACTTCTGAGACGGGT+TGG + Chr4:66159993-66160012 None:intergenic 50.0%
GCTGCCTAGGAATTACCAGC+AGG - Chr4:66160226-66160245 MsG0480021935.01.T01:intron 55.0%
! AAATGGTGGGACCCCTTCTC+GGG - Chr4:66159897-66159916 MsG0480021935.01.T01:intron 55.0%
! ACGCAGGAGCTTTAGTGCAC+CGG - Chr4:66159928-66159947 MsG0480021935.01.T01:intron 55.0%
! GAGAAGGGGTCCCACCATTT+TGG + Chr4:66159897-66159916 None:intergenic 55.0%
CTCCCAACGCCTTGGCGTAA+CGG - Chr4:66159777-66159796 MsG0480021935.01.T01:intron 60.0%
GATCCCAGGCTAGATGAGCG+AGG + Chr4:66161926-66161945 None:intergenic 60.0%
TCCCAACGCCTTGGCGTAAC+GGG - Chr4:66159778-66159797 MsG0480021935.01.T01:intron 60.0%
TTCCCGTTACGCCAAGGCGT+TGG + Chr4:66159783-66159802 None:intergenic 60.0%
! CGCAGGAGCTTTAGTGCACC+GGG - Chr4:66159929-66159948 MsG0480021935.01.T01:intron 60.0%
! TCCCGTTACGCCAAGGCGTT+GGG + Chr4:66159782-66159801 None:intergenic 60.0%
! TGCACCGGGCTTTAGTGCAC+CGG - Chr4:66159943-66159962 MsG0480021935.01.T01:intron 60.0%
CAGCCCGGTGCACTAAAGCC+CGG + Chr4:66159950-66159969 None:intergenic 65.0%
CGTGTGCAGAGCCCGAGAAG+GGG + Chr4:66159911-66159930 None:intergenic 65.0%
GCGTGTGCAGAGCCCGAGAA+GGG + Chr4:66159912-66159931 None:intergenic 65.0%
TGCGTGTGCAGAGCCCGAGA+AGG + Chr4:66159913-66159932 None:intergenic 65.0%
TTCTCGGGCTCTGCACACGC+AGG - Chr4:66159912-66159931 MsG0480021935.01.T01:intron 65.0%
! GCACCGGGCTTTAGTGCACC+GGG - Chr4:66159944-66159963 MsG0480021935.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 66159149 66163655 66159149 ID=MsG0480021935.01;Name=MsG0480021935.01
Chr4 mRNA 66159149 66163655 66159149 ID=MsG0480021935.01.T01;Parent=MsG0480021935.01;Name=MsG0480021935.01.T01;_AED=0.42;_eAED=0.44;_QI=0|0|0|1|1|1|8|0|277
Chr4 exon 66163538 66163655 66163538 ID=MsG0480021935.01.T01:exon:7335;Parent=MsG0480021935.01.T01
Chr4 exon 66162530 66162648 66162530 ID=MsG0480021935.01.T01:exon:7334;Parent=MsG0480021935.01.T01
Chr4 exon 66162328 66162425 66162328 ID=MsG0480021935.01.T01:exon:7333;Parent=MsG0480021935.01.T01
Chr4 exon 66162051 66162138 66162051 ID=MsG0480021935.01.T01:exon:7332;Parent=MsG0480021935.01.T01
Chr4 exon 66161823 66161966 66161823 ID=MsG0480021935.01.T01:exon:7331;Parent=MsG0480021935.01.T01
Chr4 exon 66161617 66161704 66161617 ID=MsG0480021935.01.T01:exon:7330;Parent=MsG0480021935.01.T01
Chr4 exon 66160374 66160507 66160374 ID=MsG0480021935.01.T01:exon:7329;Parent=MsG0480021935.01.T01
Chr4 exon 66159149 66159193 66159149 ID=MsG0480021935.01.T01:exon:7328;Parent=MsG0480021935.01.T01
Chr4 CDS 66163538 66163655 66163538 ID=MsG0480021935.01.T01:cds;Parent=MsG0480021935.01.T01
Chr4 CDS 66162530 66162648 66162530 ID=MsG0480021935.01.T01:cds;Parent=MsG0480021935.01.T01
Chr4 CDS 66162328 66162425 66162328 ID=MsG0480021935.01.T01:cds;Parent=MsG0480021935.01.T01
Chr4 CDS 66162051 66162138 66162051 ID=MsG0480021935.01.T01:cds;Parent=MsG0480021935.01.T01
Chr4 CDS 66161823 66161966 66161823 ID=MsG0480021935.01.T01:cds;Parent=MsG0480021935.01.T01
Chr4 CDS 66161617 66161704 66161617 ID=MsG0480021935.01.T01:cds;Parent=MsG0480021935.01.T01
Chr4 CDS 66160374 66160507 66160374 ID=MsG0480021935.01.T01:cds;Parent=MsG0480021935.01.T01
Chr4 CDS 66159149 66159193 66159149 ID=MsG0480021935.01.T01:cds;Parent=MsG0480021935.01.T01
Gene Sequence

>MsG0480021935.01.T01

ATGGCCAAAATTACCACCGGTATTTTTTGTAGAAACCCTTACAATGTCACCGGAATTTGTAACCGAAGTTCATGCCCTTTAGCTAATAGTCGCTATGCCACCATTCGTGAAGACAATGGTGTGTTTTACCTCTACATGAAAACTATTGAAAGAGCTCATACGCCAAAGGATTTGTGGGAAAGAGTTAAGCTGCCTAGGAATTACCAGCAGGCACTTGAAGTCATTGACAAACATCTGATGTATTGGCCCAAGCTTCTTATACACAAGATAAAACAAAGATTGACCAAAATGACTCAGATGCGGATACGTATGAGGAAACTTTCTTTGAAAACAAGGGAGAAAATAACGACACTGCCAAGAAAAGAGGTTAAGAGAGAAGCTAGAAGAGAAGAGAAAGCTGAAAAAGCAGCTGTGCTAGAAAAGTCTATTGAGAAAGAGCTATTGGTACGCCTTCAAAACGGACTTTACCAGACAGGAGGTGATATATGCAACGCTAATTTTGAAGCATACAAAAAAGTTATTGATATGGTGGAGAAATCTCAAGCTGTTGAAGAAGAGGATGAAATGATTGAAGAGGAAACAGAAACAGGAACAGAATATGTTGAATTTGAAGAGGAAGATGATATAGAAATTTTGGCGCTTTTGCAATTCACGAATGGAAGCGCTGAAGATGAAGAAACTGAAGCACTTAATCAAAGGAAAGCAAAAAGGAAAATGACTTTAGCTTCAAAGAAACTGGAAAAGGATGCCTCTGATTCTAAATCAAAGAAAGCGAAGGTCCTCATTGAGGTGGAACACGACGATGCTGTCGAAAGGCAAAGAGCGGTGCTGTAG
Protein sequence

>MsG0480021935.01.T01

MAKITTGIFCRNPYNVTGICNRSSCPLANSRYATIREDNGVFYLYMKTIERAHTPKDLWERVKLPRNYQQALEVIDKHLMYWPKLLIHKIKQRLTKMTQMRIRMRKLSLKTREKITTLPRKEVKREARREEKAEKAAVLEKSIEKELLVRLQNGLYQTGGDICNANFEAYKKVIDMVEKSQAVEEEDEMIEEETETGTEYVEFEEEDDIEILALLQFTNGSAEDEETEALNQRKAKRKMTLASKKLEKDASDSKSKKAKVLIEVEHDDAVERQRAVL*