Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021988.01.T02 | XP_024632826.1 | 67.47 | 83 | 27 | 0 | 1 | 83 | 117 | 199 | 3.09E-32 | 120 |
| MsG0480021988.01.T01 | XP_024641784.2 | 74.336 | 113 | 29 | 0 | 3 | 115 | 91 | 203 | 7.44E-54 | 177 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021988.01.T01 | A0A2Z6NW35 | 67.544 | 114 | 37 | 0 | 3 | 116 | 282 | 395 | 3.52e-49 | 170 |
| MsG0480021988.01.T02 | A0A2Z6NW35 | 65.060 | 83 | 29 | 0 | 1 | 83 | 313 | 395 | 2.36e-30 | 119 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0780040274.01 | MsG0480021988.01 | PPI |
| MsG0280009322.01 | MsG0480021988.01 | PPI |
| MsG0480021988.01 | MsG0780041116.01 | PPI |
| MsG0480021988.01 | MsG0380013578.01 | PPI |
| MsG0480021988.01 | MsG0780041116.01 | PPI |
| MsG0480021988.01 | MsG0280010449.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021988.01.T02 | MTR_5g083510 | 47.368 | 76 | 40 | 0 | 8 | 83 | 17 | 92 | 9.13e-19 | 73.6 |
| MsG0480021988.01.T02 | MTR_5g031620 | 42.683 | 82 | 47 | 0 | 2 | 83 | 141 | 222 | 1.83e-17 | 73.6 |
| MsG0480021988.01.T02 | MTR_3g010950 | 45.455 | 77 | 42 | 0 | 7 | 83 | 58 | 134 | 2.73e-17 | 71.2 |
| MsG0480021988.01.T02 | MTR_7g063280 | 65.957 | 47 | 16 | 0 | 1 | 47 | 128 | 174 | 1.02e-16 | 70.5 |
| MsG0480021988.01.T02 | MTR_2g088140 | 48.485 | 66 | 34 | 0 | 6 | 71 | 49 | 114 | 3.35e-16 | 68.2 |
| MsG0480021988.01.T02 | MTR_4g036855 | 58.491 | 53 | 22 | 0 | 31 | 83 | 39 | 91 | 3.97e-15 | 64.3 |
| MsG0480021988.01.T02 | MTR_2g027020 | 43.902 | 82 | 46 | 0 | 1 | 82 | 80 | 161 | 7.73e-15 | 65.9 |
| MsG0480021988.01.T02 | MTR_1g057740 | 41.250 | 80 | 47 | 0 | 2 | 81 | 60 | 139 | 1.10e-14 | 64.7 |
| MsG0480021988.01.T02 | MTR_1g052310 | 44.872 | 78 | 43 | 0 | 4 | 81 | 45 | 122 | 2.70e-14 | 63.2 |
| MsG0480021988.01.T02 | MTR_4g098670 | 38.750 | 80 | 49 | 0 | 1 | 80 | 17 | 96 | 3.75e-14 | 62.0 |
| MsG0480021988.01.T02 | MTR_6g007540 | 54.545 | 55 | 25 | 0 | 17 | 71 | 23 | 77 | 9.05e-13 | 59.7 |
| MsG0480021988.01.T02 | MTR_7g006380 | 49.254 | 67 | 33 | 1 | 1 | 67 | 95 | 160 | 1.36e-12 | 60.1 |
| MsG0480021988.01.T02 | MTR_2g055490 | 60.000 | 45 | 18 | 0 | 39 | 83 | 68 | 112 | 2.65e-12 | 57.8 |
| MsG0480021988.01.T02 | MTR_5g022720 | 41.176 | 68 | 40 | 0 | 1 | 68 | 10 | 77 | 5.78e-12 | 59.3 |
| MsG0480021988.01.T02 | MTR_3g071490 | 50.000 | 50 | 25 | 0 | 34 | 83 | 11 | 60 | 5.91e-12 | 55.5 |
| MsG0480021988.01.T01 | MTR_7g063280 | 67.949 | 78 | 25 | 0 | 3 | 80 | 97 | 174 | 8.29e-37 | 123 |
| MsG0480021988.01.T01 | MTR_4g036855 | 49.550 | 111 | 28 | 1 | 6 | 116 | 9 | 91 | 4.07e-29 | 101 |
| MsG0480021988.01.T01 | MTR_3g465840 | 62.857 | 70 | 26 | 0 | 4 | 73 | 1 | 70 | 2.31e-27 | 99.0 |
| MsG0480021988.01.T01 | MTR_6g016265 | 72.581 | 62 | 17 | 0 | 3 | 64 | 64 | 125 | 2.75e-27 | 97.8 |
| MsG0480021988.01.T01 | MTR_1g045560 | 55.682 | 88 | 23 | 2 | 3 | 90 | 20 | 91 | 3.94e-27 | 96.3 |
| MsG0480021988.01.T01 | MTR_5g049680 | 80.769 | 52 | 9 | 1 | 3 | 53 | 56 | 107 | 1.60e-24 | 90.1 |
| MsG0480021988.01.T01 | MTR_2g027020 | 42.857 | 112 | 60 | 1 | 4 | 115 | 54 | 161 | 3.56e-24 | 91.7 |
| MsG0480021988.01.T01 | MTR_7g072030 | 75.510 | 49 | 12 | 0 | 3 | 51 | 74 | 122 | 1.99e-23 | 87.8 |
| MsG0480021988.01.T01 | MTR_6g034915 | 45.946 | 111 | 42 | 2 | 3 | 113 | 80 | 172 | 1.12e-22 | 87.4 |
| MsG0480021988.01.T01 | MTR_0015s0120 | 73.469 | 49 | 13 | 0 | 3 | 51 | 318 | 366 | 3.39e-21 | 86.7 |
| MsG0480021988.01.T01 | MTR_5g083200 | 51.389 | 72 | 35 | 0 | 3 | 74 | 123 | 194 | 4.14e-21 | 84.0 |
| MsG0480021988.01.T01 | MTR_0002s1220 | 51.389 | 72 | 35 | 0 | 3 | 74 | 123 | 194 | 4.14e-21 | 84.0 |
| MsG0480021988.01.T01 | MTR_3g111390 | 51.389 | 72 | 35 | 0 | 3 | 74 | 123 | 194 | 4.92e-21 | 83.6 |
| MsG0480021988.01.T01 | MTR_1g070820 | 51.389 | 72 | 35 | 0 | 3 | 74 | 123 | 194 | 4.92e-21 | 83.6 |
| MsG0480021988.01.T01 | MTR_1g045340 | 50.000 | 72 | 36 | 0 | 3 | 74 | 109 | 180 | 1.42e-20 | 82.0 |
| MsG0480021988.01.T01 | MTR_7g053400 | 47.191 | 89 | 13 | 1 | 3 | 91 | 200 | 254 | 1.12e-19 | 81.3 |
| MsG0480021988.01.T01 | MTR_8g028785 | 53.165 | 79 | 37 | 0 | 7 | 85 | 287 | 365 | 9.58e-19 | 80.1 |
| MsG0480021988.01.T01 | MTR_7g013520 | 38.182 | 110 | 43 | 1 | 7 | 116 | 99 | 183 | 1.28e-18 | 77.0 |
| MsG0480021988.01.T01 | MTR_5g075610 | 47.500 | 80 | 40 | 1 | 3 | 82 | 20 | 97 | 1.59e-18 | 74.7 |
| MsG0480021988.01.T01 | MTR_5g083510 | 47.368 | 76 | 40 | 0 | 41 | 116 | 17 | 92 | 3.82e-18 | 73.6 |
| MsG0480021988.01.T01 | MTR_6g013970 | 40.860 | 93 | 49 | 1 | 3 | 89 | 124 | 216 | 4.54e-18 | 76.3 |
| MsG0480021988.01.T01 | MTR_3g026270 | 86.111 | 36 | 5 | 0 | 3 | 38 | 20 | 55 | 5.85e-18 | 72.0 |
| MsG0480021988.01.T01 | MTR_5g031620 | 42.857 | 84 | 48 | 0 | 33 | 116 | 139 | 222 | 2.48e-17 | 74.3 |
| MsG0480021988.01.T01 | MTR_1g062840 | 46.479 | 71 | 38 | 0 | 3 | 73 | 5 | 75 | 5.43e-17 | 70.5 |
| MsG0480021988.01.T01 | MTR_2g055490 | 37.963 | 108 | 34 | 1 | 9 | 116 | 38 | 112 | 7.16e-17 | 70.9 |
| MsG0480021988.01.T01 | MTR_8g088220 | 44.444 | 63 | 35 | 0 | 1 | 63 | 17 | 79 | 8.56e-17 | 69.7 |
| MsG0480021988.01.T01 | MTR_3g010950 | 44.304 | 79 | 44 | 0 | 38 | 116 | 56 | 134 | 1.01e-16 | 70.9 |
| MsG0480021988.01.T01 | MTR_6g089560 | 33.628 | 113 | 53 | 3 | 4 | 116 | 142 | 232 | 1.97e-16 | 72.4 |
| MsG0480021988.01.T01 | MTR_1g069475 | 56.364 | 55 | 24 | 0 | 3 | 57 | 189 | 243 | 2.18e-16 | 72.4 |
| MsG0480021988.01.T01 | MTR_4g029360 | 46.575 | 73 | 39 | 0 | 6 | 78 | 155 | 227 | 6.58e-16 | 70.9 |
| MsG0480021988.01.T01 | MTR_6g453270 | 57.143 | 56 | 24 | 0 | 2 | 57 | 324 | 379 | 8.42e-16 | 72.0 |
| MsG0480021988.01.T01 | MTR_5g014420 | 65.909 | 44 | 15 | 0 | 15 | 58 | 72 | 115 | 9.25e-16 | 68.2 |
| MsG0480021988.01.T01 | MTR_2g088140 | 48.485 | 66 | 34 | 0 | 39 | 104 | 49 | 114 | 9.45e-16 | 68.2 |
| MsG0480021988.01.T01 | MTR_8g098925 | 41.096 | 73 | 43 | 0 | 3 | 75 | 90 | 162 | 1.18e-15 | 68.9 |
| MsG0480021988.01.T01 | MTR_1g088650 | 43.038 | 79 | 45 | 0 | 3 | 81 | 111 | 189 | 1.48e-15 | 69.3 |
| MsG0480021988.01.T01 | MTR_5g069700 | 45.714 | 70 | 38 | 0 | 3 | 72 | 6 | 75 | 1.99e-15 | 66.2 |
| MsG0480021988.01.T01 | MTR_1g074090 | 61.905 | 42 | 16 | 0 | 3 | 44 | 106 | 147 | 5.52e-15 | 67.0 |
| MsG0480021988.01.T01 | MTR_3g116210 | 40.299 | 67 | 40 | 0 | 3 | 69 | 122 | 188 | 1.04e-14 | 67.0 |
| MsG0480021988.01.T01 | MTR_6g045427 | 45.000 | 60 | 33 | 0 | 10 | 69 | 177 | 236 | 1.37e-14 | 67.4 |
| MsG0480021988.01.T01 | MTR_1g052310 | 34.821 | 112 | 73 | 0 | 3 | 114 | 11 | 122 | 2.19e-14 | 64.7 |
| MsG0480021988.01.T01 | MTR_1g081930 | 46.875 | 64 | 34 | 0 | 1 | 64 | 61 | 124 | 2.52e-14 | 64.7 |
| MsG0480021988.01.T01 | MTR_6g016135 | 69.231 | 39 | 12 | 0 | 3 | 41 | 404 | 442 | 3.29e-14 | 67.8 |
| MsG0480021988.01.T01 | MTR_1g057740 | 41.250 | 80 | 47 | 0 | 35 | 114 | 60 | 139 | 3.34e-14 | 64.7 |
| MsG0480021988.01.T01 | MTR_4g098670 | 38.272 | 81 | 50 | 0 | 33 | 113 | 16 | 96 | 5.71e-14 | 62.8 |
| MsG0480021988.01.T01 | MTR_5g091440 | 30.172 | 116 | 48 | 1 | 1 | 116 | 18 | 100 | 6.76e-14 | 63.5 |
| MsG0480021988.01.T01 | MTR_8g036690 | 37.500 | 80 | 34 | 1 | 9 | 88 | 2 | 65 | 1.06e-13 | 61.6 |
| MsG0480021988.01.T01 | MTR_4g064590 | 33.621 | 116 | 71 | 2 | 3 | 116 | 51 | 162 | 2.01e-13 | 63.2 |
| MsG0480021988.01.T01 | MTR_8g031080 | 34.234 | 111 | 47 | 2 | 3 | 113 | 314 | 398 | 8.21e-13 | 63.5 |
| MsG0480021988.01.T01 | MTR_1g034430 | 41.935 | 62 | 36 | 0 | 3 | 64 | 120 | 181 | 8.79e-13 | 61.6 |
| MsG0480021988.01.T01 | MTR_8g059135 | 50.000 | 64 | 28 | 1 | 3 | 62 | 53 | 116 | 2.16e-12 | 59.7 |
| MsG0480021988.01.T01 | MTR_2g059470 | 42.373 | 59 | 34 | 0 | 3 | 61 | 25 | 83 | 3.73e-12 | 57.8 |
| MsG0480021988.01.T01 | MTR_6g007540 | 54.545 | 55 | 25 | 0 | 50 | 104 | 23 | 77 | 3.78e-12 | 59.3 |
| MsG0480021988.01.T01 | MTR_5g037920 | 32.353 | 102 | 67 | 2 | 3 | 103 | 123 | 223 | 4.11e-12 | 60.8 |
| MsG0480021988.01.T01 | MTR_7g006380 | 49.254 | 67 | 33 | 1 | 34 | 100 | 95 | 160 | 4.50e-12 | 60.1 |
| MsG0480021988.01.T01 | MTR_8g022750 | 40.580 | 69 | 41 | 0 | 7 | 75 | 217 | 285 | 4.60e-12 | 61.2 |
| MsG0480021988.01.T01 | MTR_0093s0070 | 43.103 | 58 | 33 | 0 | 3 | 60 | 148 | 205 | 5.84e-12 | 60.1 |
| MsG0480021988.01.T01 | MTR_1g050488 | 60.976 | 41 | 16 | 0 | 3 | 43 | 123 | 163 | 8.95e-12 | 58.9 |
| MsG0480021988.01.T01 | MTR_5g022720 | 40.278 | 72 | 43 | 0 | 30 | 101 | 6 | 77 | 1.19e-11 | 60.1 |
| MsG0480021988.01.T01 | MTR_5g085210 | 34.831 | 89 | 42 | 1 | 3 | 91 | 144 | 216 | 2.62e-11 | 58.5 |
| MsG0480021988.01.T01 | MTR_3g071490 | 50.000 | 50 | 25 | 0 | 67 | 116 | 11 | 60 | 2.70e-11 | 55.1 |
| MsG0480021988.01.T01 | MTR_3g090920 | 32.381 | 105 | 55 | 2 | 3 | 107 | 12 | 100 | 2.75e-11 | 56.2 |
| MsG0480021988.01.T01 | MTR_2g047100 | 47.917 | 48 | 25 | 0 | 30 | 77 | 55 | 102 | 4.77e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGACATGATGTTACTGAATT+TGG | 0.121195 | 4:-66858011 | MsG0480021988.01.T01:CDS |
| AAAATTGTAAAACTCTCTTT+AGG | 0.317229 | 4:-66857977 | MsG0480021988.01.T01:CDS |
| TCCAATATGAACTTCACATA+TGG | 0.322100 | 4:+66858142 | None:intergenic |
| AGAAGCTCTTGGGTTGATTT+CGG | 0.350767 | 4:-66858120 | MsG0480021988.01.T01:CDS |
| CCCATATGTGAAGTTCATAT+TGG | 0.375657 | 4:-66858143 | MsG0480021988.01.T01:CDS |
| GTTCATATTGGAGAAGCTCT+TGG | 0.432754 | 4:-66858131 | MsG0480021988.01.T01:CDS |
| TTCATATTGGAGAAGCTCTT+GGG | 0.458358 | 4:-66858130 | MsG0480021988.01.T01:CDS |
| GCATCCATCTCAAAGTCAAT+AGG | 0.467106 | 4:+66858065 | None:intergenic |
| ATGGTATTTCAAATAGAACT+TGG | 0.495246 | 4:+66857859 | None:intergenic |
| GTGGGTGCATGAGCTCAACT+TGG | 0.498228 | 4:-66858090 | MsG0480021988.01.T01:CDS |
| GGTTGATTTCGGCACTAGAG+TGG | 0.512299 | 4:-66858109 | MsG0480021988.01.T01:CDS |
| CCAATATGAACTTCACATAT+GGG | 0.513252 | 4:+66858143 | None:intergenic |
| ACTCGACTGTTGAGTTTGTA+CGG | 0.529590 | 4:-66857941 | MsG0480021988.01.T01:CDS |
| GGGACCTATTGACTTTGAGA+TGG | 0.554163 | 4:-66858069 | MsG0480021988.01.T01:CDS |
| TGTACGGAGACAAGCAAATG+AGG | 0.559576 | 4:-66857925 | MsG0480021988.01.T01:CDS |
| GGTTGCTCATAAATTAACTA+AGG | 0.561677 | 4:-66857904 | MsG0480021988.01.T01:CDS |
| GTTGATTTCGGCACTAGAGT+GGG | 0.579479 | 4:-66858108 | MsG0480021988.01.T01:CDS |
| TGCTCATAAATTAACTAAGG+TGG | 0.588501 | 4:-66857901 | MsG0480021988.01.T01:CDS |
| TGGGTGCATGAGCTCAACTT+GGG | 0.618993 | 4:-66858089 | MsG0480021988.01.T01:CDS |
| GATGGATGCGAAGAGAGTGG+TGG | 0.665544 | 4:-66858051 | MsG0480021988.01.T01:CDS |
| TGAGATGGATGCGAAGAGAG+TGG | 0.706463 | 4:-66858054 | MsG0480021988.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTGTAAAACTCTCTTT+AGG | - | Chr4:66857993-66858012 | MsG0480021988.01.T01:CDS | 20.0% |
| !!! | AAAGTGTTTTCAATACAATA+TGG | + | Chr4:66858133-66858152 | None:intergenic | 20.0% |
| !!! | TAAAGAGAGTTTTACAATTT+TGG | + | Chr4:66857994-66858013 | None:intergenic | 20.0% |
| ! | ATGGTATTTCAAATAGAACT+TGG | + | Chr4:66858114-66858133 | None:intergenic | 25.0% |
| CCAATATGAACTTCACATAT+GGG | + | Chr4:66857830-66857849 | None:intergenic | 30.0% | |
| GGTTGCTCATAAATTAACTA+AGG | - | Chr4:66858066-66858085 | MsG0480021988.01.T01:CDS | 30.0% | |
| TCCAATATGAACTTCACATA+TGG | + | Chr4:66857831-66857850 | None:intergenic | 30.0% | |
| TGCTCATAAATTAACTAAGG+TGG | - | Chr4:66858069-66858088 | MsG0480021988.01.T01:CDS | 30.0% | |
| CCCATATGTGAAGTTCATAT+TGG | - | Chr4:66857827-66857846 | MsG0480021988.01.T01:CDS | 35.0% | |
| CGACATGATGTTACTGAATT+TGG | - | Chr4:66857959-66857978 | MsG0480021988.01.T01:CDS | 35.0% | |
| TTCATATTGGAGAAGCTCTT+GGG | - | Chr4:66857840-66857859 | MsG0480021988.01.T01:CDS | 35.0% | |
| ACTCGACTGTTGAGTTTGTA+CGG | - | Chr4:66858029-66858048 | MsG0480021988.01.T01:CDS | 40.0% | |
| GCATCCATCTCAAAGTCAAT+AGG | + | Chr4:66857908-66857927 | None:intergenic | 40.0% | |
| GTTCATATTGGAGAAGCTCT+TGG | - | Chr4:66857839-66857858 | MsG0480021988.01.T01:CDS | 40.0% | |
| !! | AGAAGCTCTTGGGTTGATTT+CGG | - | Chr4:66857850-66857869 | MsG0480021988.01.T01:CDS | 40.0% |
| TGTACGGAGACAAGCAAATG+AGG | - | Chr4:66858045-66858064 | MsG0480021988.01.T01:CDS | 45.0% | |
| ! | GTTGATTTCGGCACTAGAGT+GGG | - | Chr4:66857862-66857881 | MsG0480021988.01.T01:CDS | 45.0% |
| !! | GGGACCTATTGACTTTGAGA+TGG | - | Chr4:66857901-66857920 | MsG0480021988.01.T01:CDS | 45.0% |
| TGAGATGGATGCGAAGAGAG+TGG | - | Chr4:66857916-66857935 | MsG0480021988.01.T01:CDS | 50.0% | |
| TGGGTGCATGAGCTCAACTT+GGG | - | Chr4:66857881-66857900 | MsG0480021988.01.T01:CDS | 50.0% | |
| ! | GGTTGATTTCGGCACTAGAG+TGG | - | Chr4:66857861-66857880 | MsG0480021988.01.T01:CDS | 50.0% |
| GATGGATGCGAAGAGAGTGG+TGG | - | Chr4:66857919-66857938 | MsG0480021988.01.T01:CDS | 55.0% | |
| GTGGGTGCATGAGCTCAACT+TGG | - | Chr4:66857880-66857899 | MsG0480021988.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 66857821 | 66858171 | 66857821 | ID=MsG0480021988.01;Name=MsG0480021988.01 |
| Chr4 | mRNA | 66857821 | 66858171 | 66857821 | ID=MsG0480021988.01.T01;Parent=MsG0480021988.01;Name=MsG0480021988.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|116 |
| Chr4 | exon | 66857821 | 66858171 | 66857821 | ID=MsG0480021988.01.T01:exon:20998;Parent=MsG0480021988.01.T01 |
| Chr4 | CDS | 66857821 | 66858171 | 66857821 | ID=MsG0480021988.01.T01:cds;Parent=MsG0480021988.01.T01 |
| Chr4 | mRNA | 66857821 | 66858072 | 66857821 | ID=MsG0480021988.01.T02;Parent=MsG0480021988.01;Name=MsG0480021988.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|83 |
| Chr4 | exon | 66857821 | 66858072 | 66857821 | ID=MsG0480021988.01.T02:exon:20999;Parent=MsG0480021988.01.T02 |
| Chr4 | CDS | 66857821 | 66858072 | 66857821 | ID=MsG0480021988.01.T02:cds;Parent=MsG0480021988.01.T02 |
| Gene Sequence |
| Protein sequence |
