AlfalfaGEDB | MsG0480021988.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021988.01

KEGG: -
GO: GO:0003676
PFAM: PF13456

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021988.01.T01	XP_024641784.2	74.336	113	29	0	3	115	91	203	7.44E-54	177
MsG0480021988.01.T02	XP_024632826.1	67.47	83	27	0	1	83	117	199	3.09E-32	120

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021988.01.T01	A0A2Z6NW35	67.544	114	37	0	3	116	282	395	3.52e-49	170
MsG0480021988.01.T02	A0A2Z6NW35	65.060	83	29	0	1	83	313	395	2.36e-30	119

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
MsG0280009322.01	MsG0480021988.01	PPI
MsG0480021988.01	MsG0780041116.01	PPI
MsG0480021988.01	MsG0380013578.01	PPI
MsG0480021988.01	MsG0780041116.01	PPI
MsG0480021988.01	MsG0280010449.01	PPI
MsG0780040274.01	MsG0480021988.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021988.01.T01	MTR_7g063280	67.949	78	25	0	3	80	97	174	8.29e-37	123
MsG0480021988.01.T01	MTR_4g036855	49.550	111	28	1	6	116	9	91	4.07e-29	101
MsG0480021988.01.T01	MTR_3g465840	62.857	70	26	0	4	73	1	70	2.31e-27	99.0
MsG0480021988.01.T01	MTR_6g016265	72.581	62	17	0	3	64	64	125	2.75e-27	97.8
MsG0480021988.01.T01	MTR_1g045560	55.682	88	23	2	3	90	20	91	3.94e-27	96.3
MsG0480021988.01.T01	MTR_5g049680	80.769	52	9	1	3	53	56	107	1.60e-24	90.1
MsG0480021988.01.T01	MTR_2g027020	42.857	112	60	1	4	115	54	161	3.56e-24	91.7
MsG0480021988.01.T01	MTR_7g072030	75.510	49	12	0	3	51	74	122	1.99e-23	87.8
MsG0480021988.01.T01	MTR_6g034915	45.946	111	42	2	3	113	80	172	1.12e-22	87.4
MsG0480021988.01.T01	MTR_0015s0120	73.469	49	13	0	3	51	318	366	3.39e-21	86.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 21 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGTAAAACTCTCTTT+AGG	0.317229	4:-66857977	MsG0480021988.01.T01:CDS
ACTCGACTGTTGAGTTTGTA+CGG	0.529590	4:-66857941	MsG0480021988.01.T01:CDS
AGAAGCTCTTGGGTTGATTT+CGG	0.350767	4:-66858120	MsG0480021988.01.T01:CDS
ATGGTATTTCAAATAGAACT+TGG	0.495246	4:+66857859	None:intergenic
CCAATATGAACTTCACATAT+GGG	0.513252	4:+66858143	None:intergenic
CCCATATGTGAAGTTCATAT+TGG	0.375657	4:-66858143	MsG0480021988.01.T01:CDS
CGACATGATGTTACTGAATT+TGG	0.121195	4:-66858011	MsG0480021988.01.T01:CDS
GATGGATGCGAAGAGAGTGG+TGG	0.665544	4:-66858051	MsG0480021988.01.T01:CDS
GCATCCATCTCAAAGTCAAT+AGG	0.467106	4:+66858065	None:intergenic
GGGACCTATTGACTTTGAGA+TGG	0.554163	4:-66858069	MsG0480021988.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CCAATATGAACTTCACATAT+GGG	+	Chr4:66857830-66857849	None:intergenic	30.0%
GGTTGCTCATAAATTAACTA+AGG	-	Chr4:66858066-66858085	MsG0480021988.01.T01:CDS	30.0%
TCCAATATGAACTTCACATA+TGG	+	Chr4:66857831-66857850	None:intergenic	30.0%
TGCTCATAAATTAACTAAGG+TGG	-	Chr4:66858069-66858088	MsG0480021988.01.T01:CDS	30.0%
CCCATATGTGAAGTTCATAT+TGG	-	Chr4:66857827-66857846	MsG0480021988.01.T01:CDS	35.0%
CGACATGATGTTACTGAATT+TGG	-	Chr4:66857959-66857978	MsG0480021988.01.T01:CDS	35.0%
TTCATATTGGAGAAGCTCTT+GGG	-	Chr4:66857840-66857859	MsG0480021988.01.T01:CDS	35.0%
ACTCGACTGTTGAGTTTGTA+CGG	-	Chr4:66858029-66858048	MsG0480021988.01.T01:CDS	40.0%
GCATCCATCTCAAAGTCAAT+AGG	+	Chr4:66857908-66857927	None:intergenic	40.0%
GTTCATATTGGAGAAGCTCT+TGG	-	Chr4:66857839-66857858	MsG0480021988.01.T01:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	66857821	66858171	66857821	ID=MsG0480021988.01;Name=MsG0480021988.01
Chr4	mRNA	66857821	66858171	66857821	ID=MsG0480021988.01.T01;Parent=MsG0480021988.01;Name=MsG0480021988.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|116
Chr4	exon	66857821	66858171	66857821	ID=MsG0480021988.01.T01:exon:20998;Parent=MsG0480021988.01.T01
Chr4	CDS	66857821	66858171	66857821	ID=MsG0480021988.01.T01:cds;Parent=MsG0480021988.01.T01
Chr4	mRNA	66857821	66858072	66857821	ID=MsG0480021988.01.T02;Parent=MsG0480021988.01;Name=MsG0480021988.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|83
Chr4	exon	66857821	66858072	66857821	ID=MsG0480021988.01.T02:exon:20999;Parent=MsG0480021988.01.T02
Chr4	CDS	66857821	66858072	66857821	ID=MsG0480021988.01.T02:cds;Parent=MsG0480021988.01.T02

Gene Sequence

>MsG0480021988.01.T02

ATGGATGCGAAGAGAGTGGTGGATAGTTTTTTATCTTCTCGACATGATGTTACTGAATTTGGTATCATTATCCAAAATTGTAAAACTCTCTTTAGGCATTATTATGAAAACTCGACTGTTGAGTTTGTACGGAGACAAGCAAATGAGGTTGCTCATAAATTAACTAAGGTGGTCTTATTGTCAACTAGTCTCCAAGTTCTATTTGAAATACCATATTGTATTGAAAACACTTTGATTAATAAAATGTTATAA

>MsG0480021988.01.T01

ATGCTACCCATATGTGAAGTTCATATTGGAGAAGCTCTTGGGTTGATTTCGGCACTAGAGTGGGTGCATGAGCTCAACTTGGGACCTATTGACTTTGAGATGGATGCGAAGAGAGTGGTGGATAGTTTTTTATCTTCTCGACATGATGTTACTGAATTTGGTATCATTATCCAAAATTGTAAAACTCTCTTTAGGCATTATTATGAAAACTCGACTGTTGAGTTTGTACGGAGACAAGCAAATGAGGTTGCTCATAAATTAACTAAGGTGGTCTTATTGTCAACTAGTCTCCAAGTTCTATTTGAAATACCATATTGTATTGAAAACACTTTGATTAATAAAATGTTATAA

Protein sequence

>MsG0480021988.01.T02

MDAKRVVDSFLSSRHDVTEFGIIIQNCKTLFRHYYENSTVEFVRRQANEVAHKLTKVVLLSTSLQVLFEIPYCIENTLINKML*

>MsG0480021988.01.T01

MLPICEVHIGEALGLISALEWVHELNLGPIDFEMDAKRVVDSFLSSRHDVTEFGIIIQNCKTLFRHYYENSTVEFVRRQANEVAHKLTKVVLLSTSLQVLFEIPYCIENTLINKML*